##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939101_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2133664 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.514236543335784 34.0 31.0 34.0 31.0 34.0 2 32.72780672120821 34.0 31.0 34.0 31.0 34.0 3 32.82580481275402 34.0 31.0 34.0 31.0 34.0 4 36.27177803065525 37.0 37.0 37.0 35.0 37.0 5 36.229723142912846 37.0 37.0 37.0 35.0 37.0 6 36.261782080027594 37.0 37.0 37.0 35.0 37.0 7 36.22059565142403 37.0 37.0 37.0 35.0 37.0 8 36.20341019017052 37.0 37.0 37.0 35.0 37.0 9 37.9587212419575 39.0 38.0 39.0 35.0 39.0 10-11 37.89861360551615 39.0 38.0 39.0 35.0 39.0 12-13 37.835319900415435 39.0 38.0 39.0 35.0 39.0 14-15 39.22426422342037 41.0 39.0 41.0 36.0 41.0 16-17 39.15933694339877 40.0 39.0 41.0 36.0 41.0 18-19 39.17376494143408 40.5 38.5 41.0 36.0 41.0 20-21 39.239493659732744 41.0 39.0 41.0 36.0 41.0 22-23 39.204303489209174 41.0 39.0 41.0 36.0 41.0 24-25 39.15556643407771 41.0 39.0 41.0 36.0 41.0 26-27 39.02569218958561 40.0 39.0 41.0 35.0 41.0 28-29 38.88231628785039 40.0 38.0 41.0 35.0 41.0 30-31 38.70794886167644 40.0 38.0 41.0 35.0 41.0 32-33 38.579718737345715 40.0 38.0 41.0 34.0 41.0 34-35 38.54687125058116 40.0 38.0 41.0 34.0 41.0 36-37 38.457002367757994 40.0 38.0 41.0 34.0 41.0 38-39 38.32868553811659 40.0 38.0 41.0 34.0 41.0 40-41 38.17091186803545 40.0 38.0 41.0 33.5 41.0 42-43 38.02953651559008 40.0 38.0 41.0 33.0 41.0 44-45 37.84185021634147 40.0 38.0 41.0 33.0 41.0 46-47 37.60331500179972 40.0 37.0 41.0 33.0 41.0 48-49 37.32269771622899 40.0 37.0 41.0 32.0 41.0 50-51 37.08421803995381 40.0 37.0 41.0 31.5 41.0 52-53 36.849768988931714 40.0 36.0 41.0 31.0 41.0 54-55 36.60676423279392 39.0 36.0 41.0 31.0 41.0 56-57 36.33457915585584 39.0 35.0 41.0 30.0 41.0 58-59 36.03129710207418 39.0 35.0 40.0 29.5 41.0 60-61 35.678001316046014 38.0 35.0 40.0 29.0 41.0 62-63 35.2793103787663 38.0 34.5 40.0 28.0 41.0 64-65 34.87127940481726 37.0 34.0 40.0 28.0 41.0 66-67 34.507414475756256 37.0 34.0 39.5 27.5 41.0 68-69 34.0359316180992 36.0 33.5 39.0 26.0 40.5 70-71 33.58543777277022 36.0 33.0 39.0 26.0 40.0 72-73 33.10912964740465 35.0 33.0 38.0 25.5 40.0 74-75 32.601680958201484 35.0 32.0 37.0 24.5 39.0 76-77 30.605777198284265 33.5 29.5 35.5 20.5 37.5 78-79 31.57774068456889 34.5 31.0 36.0 22.0 38.5 80-81 31.6061591703286 35.0 32.0 36.0 23.0 37.5 82-83 31.303704097739853 35.0 31.5 35.5 21.5 37.0 84-85 30.90985670658548 34.0 31.0 35.0 20.0 37.0 86-87 30.346921539661352 34.0 31.0 35.0 17.5 36.0 88-89 29.787817810114433 34.0 30.0 35.0 9.0 36.0 90-91 29.32464507063905 34.0 29.5 35.0 4.5 35.5 92-93 29.025553226749853 34.0 29.0 35.0 2.0 35.0 94-95 28.678503035154552 34.0 29.0 35.0 2.0 35.0 96-97 28.105387258724896 34.0 29.0 35.0 2.0 35.0 98-99 27.311706997915323 34.0 28.0 35.0 2.0 35.0 100-101 23.6023026587129 29.5 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10433788117736498 1101 2 0.12274837138649985 1101 3 0.16451509931075492 1101 4 0.12287257280760855 1101 5 0.09686291691106419 1101 6 0.1679716266815916 1101 7 0.11702149074252333 1101 8 0.16859179790447598 1101 9 0.2440990994025256 1101 10-11 0.1687270196268713 1101 12-13 0.19128332859896346 1101 14-15 0.18647449371485436 1101 16-17 0.16255201887937432 1101 18-19 0.2272726500464799 1101 20-21 0.23277167431670165 1101 22-23 0.28315439388251207 1101 24-25 0.24077784575172956 1101 26-27 0.2424889678944524 1101 28-29 0.20066960148795943 1101 30-31 0.23429170614515726 1101 32-33 0.19222251419608227 1101 34-35 0.2859148869598229 1101 36-37 0.40510675090033743 1101 38-39 0.38073575950240013 1101 40-41 0.4202440523925972 1101 42-43 0.4300104431985403 1101 44-45 0.38631358948698136 1101 46-47 0.35579940239937713 1101 48-49 0.29020914898471517 1101 50-51 0.3669889892330005 1101 52-53 0.33584904478110644 1101 54-55 0.3159534138253264 1101 56-57 0.22923952162009442 1101 58-59 0.26616569604221496 1101 60-61 0.3890562915757485 1101 62-63 0.3351380121145411 1101 64-65 0.2696569624609353 1101 66-67 0.16379683472721496 1101 68-69 0.2804691371789545 1101 70-71 0.29489192174288803 1101 72-73 0.1508987070341945 1101 74-75 0.2641823404719972 1101 76-77 0.21243576373655415 1101 78-79 0.3809034437286556 1101 80-81 0.4302447267380991 1101 82-83 0.4473836773113753 1101 84-85 0.30430677828728037 1101 86-87 0.4764719354807987 1101 88-89 0.6179635438448017 1101 90-91 0.5634825770024463 1101 92-93 0.4237306351349268 1101 94-95 0.2273118498362372 1101 96-97 0.18751918039612292 1101 98-99 0.12386308443714711 1101 100-101 -0.12548398138574157 1102 1 0.060781662994116914 1102 2 0.0813895673672036 1102 3 0.15023681156187507 1102 4 0.04063334584003542 1102 5 0.0662313950455129 1102 6 0.10715978009422855 1102 7 0.08524712294398995 1102 8 0.05800449896395321 1102 9 0.17203478839597608 1102 10-11 0.13970299267835173 1102 12-13 0.14460656371051783 1102 14-15 0.23652262608096208 1102 16-17 0.2300466276340032 1102 18-19 0.13065929096782014 1102 20-21 0.173764616304247 1102 22-23 0.08920113622512105 1102 24-25 0.15293046248497433 1102 26-27 0.2839111076618792 1102 28-29 0.11027547657921133 1102 30-31 0.1595560838504042 1102 32-33 0.09427181799328821 1102 34-35 0.2852212764438491 1102 36-37 0.35881700666745786 1102 38-39 0.25367079692233574 1102 40-41 0.22843986634803315 1102 42-43 0.28335904063400363 1102 44-45 0.22686023864756777 1102 46-47 0.22209861896980243 1102 48-49 0.23871045983852923 1102 50-51 0.10880039162938715 1102 52-53 0.06610391511797076 1102 54-55 0.09384881735952177 1102 56-57 0.12046485816131991 1102 58-59 0.07383814933560728 1102 60-61 0.09795468394070639 1102 62-63 0.11755855867962595 1102 64-65 0.10156402705919021 1102 66-67 0.14458200183130288 1102 68-69 0.10710700382917793 1102 70-71 -0.06693651965122172 1102 72-73 -0.14637967739977853 1102 74-75 0.012931529508193762 1102 76-77 0.12372816330665515 1102 78-79 0.12666422236600994 1102 80-81 -0.08287586200410857 1102 82-83 0.07485023337401842 1102 84-85 0.062368564271306326 1102 86-87 0.09503953868513904 1102 88-89 0.033124328367343026 1102 90-91 0.032597011527716546 1102 92-93 0.10236210655606826 1102 94-95 0.006336647463104583 1102 96-97 -0.10393273558032234 1102 98-99 -0.3338750166394213 1102 100-101 -0.7170844280989392 1103 1 0.12203809568423907 1103 2 0.15686508697700674 1103 3 0.14406969595601282 1103 4 0.11873912096627492 1103 5 0.1826115902823986 1103 6 0.14866656240579346 1103 7 0.21298329825761897 1103 8 0.20349111001267772 1103 9 0.24940894189873575 1103 10-11 0.24905700247464324 1103 12-13 0.2514887671178556 1103 14-15 0.2709357098454461 1103 16-17 0.2163759124773108 1103 18-19 0.2640596781348208 1103 20-21 0.2565232845048868 1103 22-23 0.3240888784628382 1103 24-25 0.23020059069098409 1103 26-27 0.26136793027463057 1103 28-29 0.2996469846704528 1103 30-31 0.3047891762951451 1103 32-33 0.2370558380543244 1103 34-35 0.3373482837531796 1103 36-37 0.3797364249524904 1103 38-39 0.33742769462620714 1103 40-41 0.34456929861792673 1103 42-43 0.27766313895512695 1103 44-45 0.3748579239124936 1103 46-47 0.40481526370619036 1103 48-49 0.38225090225605385 1103 50-51 0.3840230815444414 1103 52-53 0.40576442063957785 1103 54-55 0.32777139773800457 1103 56-57 0.3903319675670005 1103 58-59 0.4512508817563585 1103 60-61 0.45039652277727527 1103 62-63 0.4239971045299811 1103 64-65 0.3798764749604331 1103 66-67 0.4291063444396528 1103 68-69 0.33674939103751456 1103 70-71 0.18552391973508975 1103 72-73 0.11830683444601675 1103 74-75 0.1948242387815995 1103 76-77 0.11795234815478395 1103 78-79 0.2537900240841502 1103 80-81 0.17003443512244942 1103 82-83 0.1890534753809483 1103 84-85 0.3030390107533911 1103 86-87 0.44252674219582033 1103 88-89 0.328901314664936 1103 90-91 0.3841570465243045 1103 92-93 0.3270471028015116 1103 94-95 0.1980718031910449 1103 96-97 -0.03787873887331017 1103 98-99 0.15575001005581157 1103 100-101 -0.0822573098752244 1104 1 0.11244872943690609 1104 2 0.11590041078640922 1104 3 0.1083744239904405 1104 4 0.05773075434211705 1104 5 0.058556506542402076 1104 6 0.07863500820377567 1104 7 0.10959145621905009 1104 8 0.04258479501510948 1104 9 0.07888939049676225 1104 10-11 0.07969971315895208 1104 12-13 -0.012832321361301524 1104 14-15 0.06859388651410825 1104 16-17 0.134891778756149 1104 18-19 0.07185113878727378 1104 20-21 0.08971500611984595 1104 22-23 0.10507482323158257 1104 24-25 0.11904652223728363 1104 26-27 0.041824574425575634 1104 28-29 0.07205294899127779 1104 30-31 0.184474197503377 1104 32-33 0.22725673626955967 1104 34-35 0.14107149184864198 1104 36-37 0.2126730845519873 1104 38-39 0.3430522009631929 1104 40-41 0.2608530370063633 1104 42-43 0.2656600707012373 1104 44-45 0.27055847949710454 1104 46-47 0.2311105214569551 1104 48-49 0.13947464206597004 1104 50-51 0.23460099413943425 1104 52-53 0.19834308152376678 1104 54-55 0.30812746538442326 1104 56-57 0.3941003680385009 1104 58-59 0.3341555580274118 1104 60-61 0.3322679430918569 1104 62-63 0.24927788060511347 1104 64-65 0.18549709233924716 1104 66-67 0.247841735702373 1104 68-69 0.08558129551397542 1104 70-71 0.012981573674139213 1104 72-73 0.03773647179396278 1104 74-75 0.151115299139569 1104 76-77 -0.0065531842234705096 1104 78-79 0.22792737940484642 1104 80-81 0.17775093275353981 1104 82-83 0.11050402995979525 1104 84-85 0.07633782623036822 1104 86-87 0.35525463179232375 1104 88-89 0.41477487524753087 1104 90-91 0.41694082983692127 1104 92-93 0.36553960851090395 1104 94-95 0.37343954441708505 1104 96-97 0.29703431609107867 1104 98-99 0.1930089114117166 1104 100-101 -0.19145308673484962 1105 1 0.02946855171988716 1105 2 0.048145507349083516 1105 3 0.052467321874594575 1105 4 0.08266983548102047 1105 5 0.12655238220999365 1105 6 0.03569483805575402 1105 7 0.10483302701815944 1105 8 0.09010189284443015 1105 9 0.1057979562099689 1105 10-11 0.111915846713174 1105 12-13 0.09392841715679623 1105 14-15 0.11722702560219034 1105 16-17 0.0358626187992499 1105 18-19 0.07041302887459011 1105 20-21 0.10479932709176865 1105 22-23 0.11654456074949593 1105 24-25 0.02905150387472588 1105 26-27 0.0661913465175914 1105 28-29 0.0033743186712484885 1105 30-31 0.08116353255131514 1105 32-33 0.02041567367471231 1105 34-35 0.05044237668786167 1105 36-37 -0.0014319005610374802 1105 38-39 0.06393259918525018 1105 40-41 0.08755731696666658 1105 42-43 0.007650640345680415 1105 44-45 0.14795188597168618 1105 46-47 -0.010476883109937773 1105 48-49 0.1524446634213632 1105 50-51 0.221460670294384 1105 52-53 0.11459982507756905 1105 54-55 0.07871533722118329 1105 56-57 0.0011553707224081222 1105 58-59 0.02482908313448462 1105 60-61 0.07546376006109057 1105 62-63 0.030164612071686747 1105 64-65 -0.04036996359025835 1105 66-67 -0.035207180888029654 1105 68-69 -0.07493517285263351 1105 70-71 -0.0028720048021000366 1105 72-73 -0.008849175683742772 1105 74-75 -0.1631974472873523 1105 76-77 -0.04835375653251717 1105 78-79 0.17439833336911192 1105 80-81 0.028961779593288384 1105 82-83 0.023720429973700163 1105 84-85 0.12573674726176165 1105 86-87 0.18811721969505513 1105 88-89 0.31761788415654735 1105 90-91 0.15098665317801263 1105 92-93 0.1950231787616481 1105 94-95 0.021055502648561486 1105 96-97 -0.08139287669828832 1105 98-99 0.04565362025624964 1105 100-101 -0.33765101607282944 1106 1 0.006217381167346048 1106 2 -0.0073855299452816325 1106 3 0.0029496030306930265 1106 4 -0.004403555983920171 1106 5 0.022741409408077118 1106 6 0.005809492649120784 1106 7 0.009983949400059089 1106 8 0.007847751857468666 1106 9 0.01591130916609984 1106 10-11 0.04664291425955014 1106 12-13 0.0864535811201037 1106 14-15 0.018946783104723863 1106 16-17 0.051354926245963384 1106 18-19 0.018347048146189593 1106 20-21 0.05388455854374996 1106 22-23 0.08984454269848641 1106 24-25 0.03575640920517742 1106 26-27 0.041288693019566836 1106 28-29 0.09602706764314206 1106 30-31 0.061803029382566876 1106 32-33 0.034643796652410686 1106 34-35 -0.009188572316197963 1106 36-37 0.0564372947201619 1106 38-39 0.04672790199396104 1106 40-41 -0.07232867925858244 1106 42-43 -0.028706543648105765 1106 44-45 -0.06550446436029489 1106 46-47 -0.05610454854986813 1106 48-49 -0.0632622913737535 1106 50-51 -0.12710083636299885 1106 52-53 -0.009871568988195634 1106 54-55 -0.09680737450669596 1106 56-57 -0.07348063133566285 1106 58-59 -0.005098012900560889 1106 60-61 -0.11803714064732418 1106 62-63 -0.17631324351780364 1106 64-65 -0.06905387326645496 1106 66-67 -0.020308714732436783 1106 68-69 -0.15238062381467898 1106 70-71 -0.05342808633371021 1106 72-73 -0.03210476689106656 1106 74-75 -0.1730218613357053 1106 76-77 0.08480459254939987 1106 78-79 0.18901363202731858 1106 80-81 0.0434884195641132 1106 82-83 0.08092775499865823 1106 84-85 0.032310997684486864 1106 86-87 0.09562619301026132 1106 88-89 0.09719310835128425 1106 90-91 0.19812053393576434 1106 92-93 0.09137920847335934 1106 94-95 -0.17327549878937631 1106 96-97 -0.3764453044359328 1106 98-99 -0.14550507019845327 1106 100-101 0.06481939316928376 1107 1 -0.09401749608159093 1107 2 -0.06620656726833829 1107 3 0.01855327442083876 1107 4 -0.006190566623409666 1107 5 0.012688337633484537 1107 6 0.08414547123371818 1107 7 -0.03420949869034473 1107 8 -0.03725092985717282 1107 9 0.05161633704236124 1107 10-11 0.0147583217331686 1107 12-13 0.025341096387847983 1107 14-15 0.051885606593018 1107 16-17 0.14618556833233498 1107 18-19 0.03677029967739287 1107 20-21 0.01463889915333283 1107 22-23 0.112384429106271 1107 24-25 0.09156722160089714 1107 26-27 0.09646928856371773 1107 28-29 0.14291491417564117 1107 30-31 0.16327979046527474 1107 32-33 0.14413924738671113 1107 34-35 0.09394451258452108 1107 36-37 0.06632126349223455 1107 38-39 0.08293210087058611 1107 40-41 -0.008957418822483021 1107 42-43 0.08917332835540037 1107 44-45 -0.08180692728030436 1107 46-47 -0.0990747823524245 1107 48-49 0.08980357119397553 1107 50-51 0.018273292959989362 1107 52-53 -0.14238704855497275 1107 54-55 -0.1362394900032058 1107 56-57 -0.022307046924055385 1107 58-59 -0.11274469988192237 1107 60-61 -0.1720313822891697 1107 62-63 -0.21604838010588168 1107 64-65 -0.05952079621652473 1107 66-67 -0.0955903077329836 1107 68-69 -0.04437550548685465 1107 70-71 0.12818279389469467 1107 72-73 0.08754679715014646 1107 74-75 0.09606122699972985 1107 76-77 0.0458282054559902 1107 78-79 0.2296876313576135 1107 80-81 0.08105010984735728 1107 82-83 5.53205610888341E-4 1107 84-85 0.10101911537060104 1107 86-87 0.11205407698238545 1107 88-89 0.2508274346571291 1107 90-91 0.23435800290063824 1107 92-93 0.28568669637346034 1107 94-95 0.20741796473930663 1107 96-97 0.05197828937027893 1107 98-99 0.06256007790011253 1107 100-101 -0.2655094996045726 1108 1 9.654758483961245E-4 1108 2 0.01750071354639715 1108 3 0.06260630569779124 1108 4 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84-85 0.1716757552294439 2316 86-87 -0.06981445651038598 2316 88-89 -0.18447550229465293 2316 90-91 -0.30534307038462316 2316 92-93 -0.2721092044351501 2316 94-95 0.11273900244282586 2316 96-97 -0.08280732700315596 2316 98-99 -0.023547367287239496 2316 100-101 0.08682855973234993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 16.0 5 28.0 6 64.0 7 171.0 8 374.0 9 770.0 10 1279.0 11 2180.0 12 3176.0 13 4573.0 14 5869.0 15 7723.0 16 9784.0 17 13468.0 18 13589.0 19 10615.0 20 9890.0 21 10472.0 22 11263.0 23 12323.0 24 14085.0 25 16551.0 26 19592.0 27 23781.0 28 28865.0 29 35098.0 30 42954.0 31 53428.0 32 66756.0 33 85912.0 34 119660.0 35 176630.0 36 289914.0 37 505198.0 38 485951.0 39 51654.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.212667572174084 19.326861212803024 14.90489350254533 23.555577712477564 2 15.53135826446901 20.200837620168873 40.49855084961831 23.76925326574381 3 19.65735935929931 23.72093263044228 29.47066642170463 27.151041588553777 4 13.38200391439327 16.849091515815047 36.55500584909339 33.213898720698296 5 14.20209555018972 36.758458688903225 34.34987889377146 14.689566867135595 6 32.67238890471977 35.84097589873569 17.307083027130794 14.179552169413743 7 28.9849292109723 31.931925551539514 20.63487971864361 18.44826551884458 8 25.69917287820388 36.31902680084587 19.46810744334628 18.513692877603972 9 25.640963150711638 17.611160895061264 20.384606011068286 36.36326994315881 10-11 24.920535754458058 26.692136156395758 27.216140873164658 21.171187215981526 12-13 25.704351762976742 23.641187178487336 27.81159545270483 22.842865605831097 14-15 22.499059706581583 25.424010817182353 25.836919727648844 26.240009748587216 16-17 22.373016321242126 28.45670794451054 26.65574006370831 22.51453567053902 18-19 22.32951033035676 27.27715201510267 28.497611853418274 21.895725801122296 20-21 22.826274427463744 26.453696552034433 28.812479378196382 21.907549642305444 22-23 22.53895177497488 26.361507716303972 28.603753918142687 22.495786590578458 24-25 22.471258172615002 26.543833337239004 28.637920933611415 22.34698755653458 26-27 22.314820977129205 26.75713968348895 28.57907480088 22.348964538501846 28-29 22.216056511240758 27.08734833600792 28.524078767790996 22.172516384960332 30-31 22.40624635298981 26.722806603121395 28.501563322408117 22.369383721480673 32-33 22.232835160550117 26.683114117311817 28.491083881998293 22.592966840139777 34-35 22.66633574354114 26.834162516065707 28.296669300927242 22.20283243946591 36-37 22.649836104308168 26.569104349424116 28.381356583768703 22.399702962499017 38-39 22.321012111833706 26.85949250962051 28.38970318342886 22.42979219511692 40-41 22.686088488490178 26.74266836069727 28.345622555075327 22.225620595737222 42-43 22.464007574639023 26.759498180164854 28.205546454426784 22.570947790769345 44-45 22.56576943086535 26.896474955356936 28.137130376501577 22.400625237276138 46-47 22.761141565180875 27.009015612814206 27.782555754694343 22.447287067310576 48-49 22.658764398455418 27.043928031274124 27.754119820082874 22.54318775018759 50-51 22.69661267810928 27.207730184068517 27.682430488052933 22.413226649769268 52-53 22.82341549559818 27.15282256250281 27.57699900265459 22.446762939244415 54-55 22.633577732951395 27.05507990011548 27.710361144022677 22.60098122291045 56-57 22.622891889257165 27.29593319285511 27.545761656943174 22.535413260944555 58-59 22.640581766301246 27.076688079119915 27.620283641771554 22.66244651280728 60-61 22.536465280028647 27.15392816321671 27.77309440392682 22.536512152827825 62-63 22.47686674283036 27.172299850601988 27.82719043852711 22.52364296804054 64-65 22.388117063348854 27.1320163815948 27.72523521124252 22.75463134381383 66-67 22.329931677859964 27.204252592721396 27.588116437762267 22.877699291656377 68-69 22.505390599020284 27.007785756819946 27.77522988991953 22.71159375424024 70-71 22.50764412766021 27.00057272372782 27.518578370352596 22.973204778259372 72-73 22.506425566537185 27.086715621578655 27.525561662942245 22.881297148941915 74-75 22.381757390104536 27.28674711669691 27.365531780074086 22.965963713124466 76-77 22.426269318524763 27.118893255939845 27.520124380709248 22.93471304482614 78-79 22.44980456117657 27.190953572734177 27.51145918281263 22.847782683276623 80-81 22.483349108745827 27.15157449084163 27.407832181794618 22.957244218617923 82-83 22.51638620114311 27.217802315744578 27.437847262092514 22.8279642210198 84-85 22.459698434242693 27.203955261934397 27.34181670591058 22.994529597912326 86-87 22.450767825329297 27.158903175041843 27.392612604919393 22.99771639470947 88-89 22.440383301300574 27.193059652464953 27.20988391026812 23.156673135966358 90-91 22.356551692821895 27.166179371055748 27.3370602605116 23.140208675610758 92-93 22.346091043388274 27.06478152136419 27.408814133809262 23.180313301438275 94-95 22.263791299848524 26.92063980083087 27.49425401562758 23.321314883693027 96-97 22.303722610495374 26.746619898915668 27.543441704035875 23.406215786553084 98-99 22.103868944536334 26.88403008767683 27.34193494563962 23.67016602214722 100-101 22.310081679377685 26.270529691525894 27.94353331560157 23.475855313494847 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 145.5 2 117.5 3 166.0 4 137.0 5 128.5 6 265.0 7 577.5 8 958.5 9 1206.5 10 1154.0 11 1058.0 12 1138.5 13 1234.0 14 1318.5 15 1463.0 16 1649.0 17 1822.0 18 2022.5 19 2281.0 20 2724.0 21 3305.5 22 4053.5 23 5198.5 24 6626.5 25 8490.5 26 11026.0 27 14274.0 28 17331.0 29 20385.5 30 24058.0 31 28187.0 32 33506.5 33 37686.0 34 40709.0 35 44930.0 36 49608.0 37 53945.5 38 57569.0 39 60382.0 40 63466.5 41 67444.0 42 70243.0 43 72550.0 44 76200.0 45 79573.5 46 80908.0 47 82147.5 48 85098.5 49 85032.5 50 81758.0 51 79217.5 52 77385.5 53 75846.5 54 73884.0 55 68272.0 56 61592.0 57 55645.0 58 50098.5 59 44576.0 60 36753.0 61 29777.5 62 24131.5 63 18064.5 64 12998.5 65 9536.0 66 7047.5 67 5561.5 68 4256.0 69 3205.0 70 2403.0 71 1673.5 72 1215.0 73 982.5 74 739.0 75 503.5 76 368.5 77 234.5 78 142.0 79 86.0 80 40.5 81 19.0 82 15.0 83 10.0 84 5.0 85 4.5 86 1.5 87 0.5 88 2.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3475711264753963 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001007656313271443 16-17 0.001570069139283411 18-19 4.686773550099735E-5 20-21 0.0 22-23 0.0 24-25 6.561482970139629E-4 26-27 0.001218561123025931 28-29 0.0 30-31 0.003140138278566822 32-33 0.0 34-35 0.011154521049237368 36-37 0.028120641300598406 38-39 0.021606026065959776 40-41 0.013615077163039727 42-43 0.01073271142972839 44-45 0.003468212427073804 46-47 0.0014997675360319152 48-49 7.030160325149602E-4 50-51 9.37354710019947E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.004335265533842255 60-61 0.010803013032979888 62-63 0.0047570751533512305 64-65 0.002343386775049867 66-67 0.004452434872594748 68-69 0.004147794591838266 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.221051674490454 78-79 1.874709420039894E-4 80-81 0.0032573076173193155 82-83 4.218096195089761E-4 84-85 0.0 86-87 0.11177954916987867 88-89 0.41037389204673275 90-91 0.39835231789072695 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0015466352715329122 100-101 7.733176357664561E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2133664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.42593358515015 #Duplication Level Percentage of deduplicated Percentage of total 1 78.94255108476102 49.28062451059614 2 12.721683255320816 15.883259079759476 3 3.765004852697742 7.051018286468319 4 1.5847844983292756 3.957266073579154 5 0.8173350263163897 2.5511451034821944 6 0.4800890341158036 1.7982003695203224 7 0.32618451010149474 1.4253660794870453 8 0.23096572380086233 1.1534600747551012 9 0.1698054280109268 0.9540236134267299 >10 0.8858801997277536 10.010234864286485 >50 0.0526228505548147 2.213572554764097 >100 0.021365012891621935 2.4741697385812786 >500 0.0010521446609285936 0.4212505079704619 >1k 6.763787105968632E-4 0.8264091433231858 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4713 0.2208876374162005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4021 0.18845516444951033 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2452 0.1149196874484455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.686773550099734E-5 2 4.686773550099734E-5 0.0 0.0 0.0 4.686773550099734E-5 3 4.686773550099734E-5 0.0 0.0 0.0 4.686773550099734E-5 4 4.686773550099734E-5 0.0 0.0 0.0 4.686773550099734E-5 5 4.686773550099734E-5 0.0 0.0 0.0 4.686773550099734E-5 6 4.686773550099734E-5 0.0 0.0 0.0 9.373547100199469E-5 7 9.373547100199469E-5 0.0 0.0 0.0 1.4060320650299203E-4 8 9.373547100199469E-5 0.0 0.0 0.0 1.4060320650299203E-4 9 9.373547100199469E-5 0.0 0.0 0.0 1.4060320650299203E-4 10-11 9.373547100199469E-5 0.0 0.0 0.0 1.4060320650299203E-4 12-13 9.373547100199469E-5 0.0 0.0 0.0 1.4060320650299203E-4 14-15 1.4060320650299203E-4 0.0 0.0 0.0 1.4060320650299203E-4 16-17 1.4060320650299203E-4 0.0 0.0 7.030160325149601E-5 1.4060320650299203E-4 18-19 1.640370742534907E-4 0.0 0.0 1.4060320650299203E-4 1.4060320650299203E-4 20-21 1.8747094200398937E-4 0.0 0.0 1.4060320650299203E-4 1.640370742534907E-4 22-23 1.8747094200398937E-4 0.0 0.0 1.8747094200398937E-4 2.3433867750498671E-4 24-25 1.8747094200398937E-4 0.0 0.0 3.515080162574801E-4 3.2807414850698143E-4 26-27 1.8747094200398937E-4 0.0 0.0 4.921112227604721E-4 3.2807414850698143E-4 28-29 1.8747094200398937E-4 0.0 0.0 0.0016872384780359045 3.2807414850698143E-4 30-31 1.8747094200398937E-4 0.0 0.0 0.005389789582614695 3.2807414850698143E-4 32-33 1.8747094200398937E-4 0.0 0.0 0.012302780569011802 3.2807414850698143E-4 34-35 1.8747094200398937E-4 0.0 0.0 0.02364477256025316 4.218096195089761E-4 36-37 3.2807414850698143E-4 0.0 0.0 0.0459538146587279 4.218096195089761E-4 38-39 4.218096195089761E-4 0.0 0.0 0.07557422349535822 4.218096195089761E-4 40-41 4.218096195089761E-4 0.0 0.0 0.1077020561812919 4.218096195089761E-4 42-43 4.6867735500997343E-4 0.0 0.0 0.14247791592303194 4.218096195089761E-4 44-45 5.624128260119681E-4 0.0 0.0 0.18615864540996147 4.4524348725947474E-4 46-47 5.858466937624669E-4 0.0 0.0 0.24155630877214035 4.6867735500997343E-4 48-49 6.092805615129655E-4 0.0 0.0 0.3021094230394289 4.6867735500997343E-4 50-51 6.092805615129655E-4 0.0 0.0 0.3673024431213162 4.6867735500997343E-4 52-53 6.092805615129655E-4 0.0 0.0 0.4334796856487244 5.155450905109707E-4 54-55 6.092805615129655E-4 0.0 0.0 0.510389639605861 5.624128260119681E-4 56-57 6.092805615129655E-4 0.0 0.0 0.6051327669211273 5.624128260119681E-4 58-59 6.092805615129655E-4 0.0 0.0 0.7104211347241177 5.624128260119681E-4 60-61 6.092805615129655E-4 0.0 0.0 0.8234426788847728 5.624128260119681E-4 62-63 6.092805615129655E-4 0.0 0.0 0.9407057531082682 5.624128260119681E-4 64-65 6.092805615129655E-4 0.0 0.0 1.0687718403647435 5.624128260119681E-4 66-67 6.561482970139629E-4 0.0 0.0 1.2217949967755 5.858466937624669E-4 68-69 6.561482970139629E-4 0.0 0.0 1.3961898405747108 6.092805615129655E-4 70-71 7.264499002654589E-4 0.0 0.0 1.5912064879943608 6.092805615129655E-4 72-73 8.436192390179522E-4 0.0 0.0 1.7900662897250927 6.561482970139629E-4 74-75 9.139208422694482E-4 0.0 0.0 2.0092901225310076 6.561482970139629E-4 76-77 9.373547100199469E-4 0.0 0.0 2.2469329753888148 6.561482970139629E-4 78-79 9.607885777704456E-4 0.0 0.0 2.5147117821737632 6.561482970139629E-4 80-81 0.001077957916522939 0.0 0.0 2.8081975418810083 6.561482970139629E-4 82-83 0.0011248256520239362 0.0 0.0 3.11279095490199 6.561482970139629E-4 84-85 0.0011248256520239362 0.0 0.0 3.4341864511000795 7.030160325149602E-4 86-87 0.0011482595197744349 0.0 0.0 3.7753132639440885 7.498837680159575E-4 88-89 0.001218561123025931 0.0 0.0 4.142732876404157 7.967515035169549E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4420 0.0 35.862476 1 GTATCAA 6725 0.0 32.206047 1 ATCAACG 7815 0.0 27.078056 3 TCAACGC 7990 0.0 26.425596 4 CAACGCA 8060 0.0 26.254963 5 TATCAAC 8170 0.0 25.959543 2 AACGCAG 8395 0.0 25.320303 6 ACGCAGA 9510 0.0 22.152056 7 CGCAGAG 9535 0.0 22.044214 8 GAGTACT 5955 0.0 20.0794 12-13 TACATGG 6325 0.0 19.87911 2 GTACATG 6595 0.0 19.704533 1 GTACTTT 6060 0.0 19.45744 14-15 GCAGAGT 10850 0.0 19.110561 9 GTGCGAA 125 0.0059590577 19.040497 1 ACATGGG 6265 0.0 19.009216 3 AGAGTAC 8865 0.0 18.492739 10-11 GTACCTG 1610 0.0 17.739595 1 GAGTACA 5160 0.0 17.527592 1 AGTACTT 6405 0.0 17.335192 12-13 >>END_MODULE