FastQCFastQC Report
Fri 27 May 2016
SRR939100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098087
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43940.20942887497038967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35160.16758122994899638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22260.10609664899501309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40150.035.0042151
GTATCAA62500.033.350221
TCAACGC73000.028.2249074
ATCAACG72700.028.2110733
TATCAAC74150.027.7871632
CAACGCA73900.027.7529755
AACGCAG76850.026.859086
GTACATG57200.024.3212471
TACATGG55250.024.2616462
ACGCAGA86900.023.4259477
CGCAGAG87250.023.1691958
ACATGGG57400.022.9402963
CATGGGG39000.020.1608854
GCAGAGT99100.019.920999
GTACCGT1200.004857379519.725926
GTACTTT52650.019.42360314-15
GAGTACT53650.019.37019712-13
AGAGTAC79450.019.18760710-11
GTATAGC9300.018.8900451
GTGTAGC13800.018.5792791
Produced by FastQC (version 0.11.5)