##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939100_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2098087 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.039926371022744 33.0 31.0 34.0 30.0 34.0 2 32.15546400125448 34.0 31.0 34.0 30.0 34.0 3 32.219406535572645 34.0 31.0 34.0 30.0 34.0 4 35.64767666927062 37.0 35.0 37.0 35.0 37.0 5 35.68209897873634 37.0 35.0 37.0 35.0 37.0 6 35.75299117720095 37.0 36.0 37.0 35.0 37.0 7 35.68203940065403 37.0 36.0 37.0 35.0 37.0 8 35.67275570555463 37.0 36.0 37.0 35.0 37.0 9 37.350461634813044 39.0 38.0 39.0 35.0 39.0 10-11 37.25110779486265 39.0 37.0 39.0 34.0 39.0 12-13 37.15001451322085 39.0 37.0 39.0 34.0 39.0 14-15 38.44695572681209 40.0 38.0 41.0 34.0 41.0 16-17 38.34113242205876 40.0 38.0 41.0 33.5 41.0 18-19 38.2142384943999 40.0 38.0 41.0 33.5 41.0 20-21 38.18526066840889 40.0 38.0 41.0 33.5 41.0 22-23 38.235801947202376 40.0 38.0 41.0 34.0 41.0 24-25 38.18277411756519 40.0 38.0 41.0 33.0 41.0 26-27 38.07362754738007 40.0 38.0 41.0 33.0 41.0 28-29 37.9249795170553 40.0 38.0 41.0 33.0 41.0 30-31 37.726047346940334 40.0 38.0 41.0 32.5 41.0 32-33 37.53611861662553 40.0 37.5 41.0 32.0 41.0 34-35 37.230554071399325 40.0 37.0 41.0 31.0 41.0 36-37 36.97589637607973 40.0 37.0 41.0 30.5 41.0 38-39 36.79373066989119 40.0 37.0 41.0 30.0 41.0 40-41 36.60910558046449 40.0 36.0 41.0 30.0 41.0 42-43 36.411667390341776 40.0 36.0 41.0 30.0 41.0 44-45 36.165726683402546 39.0 36.0 41.0 29.0 41.0 46-47 36.08001550936639 39.5 36.0 41.0 28.5 41.0 48-49 35.97905329950569 40.0 36.0 41.0 28.0 41.0 50-51 34.882216037752485 38.5 34.5 40.0 25.5 40.5 52-53 35.02734062028887 38.5 34.5 39.5 26.5 40.5 54-55 35.55848232222972 39.0 35.0 41.0 27.0 41.0 56-57 35.46497476034121 39.0 35.0 41.0 27.0 41.0 58-59 35.261859017285744 39.0 35.0 41.0 26.0 41.0 60-61 34.965683024583825 38.5 35.0 40.5 26.0 41.0 62-63 34.584377578241515 38.0 34.0 40.0 25.5 41.0 64-65 34.214216093040946 37.5 34.0 40.0 24.5 41.0 66-67 33.82296206020055 37.0 34.0 40.0 23.0 41.0 68-69 33.4067262225065 36.0 33.5 39.0 22.0 41.0 70-71 32.97547027363498 36.0 33.0 39.0 21.5 41.0 72-73 32.52626892974409 35.0 33.0 38.5 20.0 40.0 74-75 32.07500785239125 35.0 32.0 37.5 18.5 39.5 76-77 31.632041950595948 35.0 32.0 37.0 17.5 39.0 78-79 31.1950841409341 35.0 31.5 36.5 12.0 39.0 80-81 30.731784954580053 35.0 31.0 36.0 7.5 38.0 82-83 30.314126868904864 35.0 31.0 35.5 4.5 37.0 84-85 29.916412188817716 34.0 30.5 35.0 2.0 37.0 86-87 29.49173318360964 34.0 30.0 35.0 2.0 36.0 88-89 29.056010308438115 34.0 29.5 35.0 2.0 36.0 90-91 28.63142591322476 34.0 29.0 35.0 2.0 36.0 92-93 28.21200026500331 34.0 29.0 35.0 2.0 35.0 94-95 27.714508978893633 34.0 28.0 35.0 2.0 35.0 96-97 27.100636198594245 34.0 26.5 35.0 2.0 35.0 98-99 26.35175662401035 33.5 25.0 35.0 2.0 35.0 100-101 23.331501267583278 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25283940952208184 1101 2 0.43946135472928916 1101 3 0.26584202189601314 1101 4 0.273683840611497 1101 5 0.19313438154271978 1101 6 0.292897596775866 1101 7 0.2591083931254232 1101 8 0.31910731787167634 1101 9 0.26277218714714934 1101 10-11 0.29278873439076136 1101 12-13 0.39361724005016185 1101 14-15 0.4207599378481248 1101 16-17 0.48003719688541935 1101 18-19 0.532194199258079 1101 20-21 0.6047155850548762 1101 22-23 0.6241496575042689 1101 24-25 0.6633231891802325 1101 26-27 0.5947543164242717 1101 28-29 0.6030337713651335 1101 30-31 0.5660117920612748 1101 32-33 0.5660828747149509 1101 34-35 0.5758361818651068 1101 36-37 0.49992998602805727 1101 38-39 0.4346290375349824 1101 40-41 0.5761234967568001 1101 42-43 0.5443048325641229 1101 44-45 0.49901425615615125 1101 46-47 0.5978616058597837 1101 48-49 0.59946369097527 1101 50-51 0.5489568730500096 1101 52-53 0.466174259327083 1101 54-55 0.5397223551115431 1101 56-57 0.6444662314539897 1101 58-59 0.4689935444473008 1101 60-61 0.4706804831914866 1101 62-63 0.44944784654007464 1101 64-65 0.3600245285821373 1101 66-67 0.537132498658238 1101 68-69 0.479870204595116 1101 70-71 0.5155429719934403 1101 72-73 0.4725454250930312 1101 74-75 0.42413473180936734 1101 76-77 0.4810760327971728 1101 78-79 0.34218723141361096 1101 80-81 0.28367611691301775 1101 82-83 0.1513299462724227 1101 84-85 0.16959440037258489 1101 86-87 0.24955339390512066 1101 88-89 0.36351264327000266 1101 90-91 0.2798850828155963 1101 92-93 0.3034133053292436 1101 94-95 0.19672185668509812 1101 96-97 0.24604836325364232 1101 98-99 0.1277369001566342 1101 100-101 -0.5309153556526489 1102 1 0.20747271947453783 1102 2 0.23755191664963604 1102 3 0.36642264694472715 1102 4 0.11537583655881178 1102 5 0.1513449652852188 1102 6 0.16313764042016743 1102 7 0.13990879839391113 1102 8 0.29272589787323966 1102 9 0.3181809627686931 1102 10-11 0.26186947634384694 1102 12-13 0.3242715148533719 1102 14-15 0.4115848930347781 1102 16-17 0.41193845165901166 1102 18-19 0.3200166604847894 1102 20-21 0.3256278288394583 1102 22-23 0.3465037218796141 1102 24-25 0.44552449850920794 1102 26-27 0.3757008521580474 1102 28-29 0.38363543920084453 1102 30-31 0.5364622635755794 1102 32-33 0.49340732331600634 1102 34-35 0.4439836372466033 1102 36-37 0.4552861546820992 1102 38-39 0.3199146738844476 1102 40-41 0.3820135287106652 1102 42-43 0.254029327926915 1102 44-45 0.3365386112336992 1102 46-47 0.5659311249931278 1102 48-49 0.5089855520928737 1102 50-51 0.4183532568080963 1102 52-53 0.3242484546353168 1102 54-55 0.36248363638346603 1102 56-57 0.4785964629246422 1102 58-59 0.29630630261112145 1102 60-61 0.3192269721635057 1102 62-63 0.363523132955784 1102 64-65 0.339862421185984 1102 66-67 0.3373059068103714 1102 68-69 0.29410915713194186 1102 70-71 0.29216209754874 1102 72-73 0.14579380791588648 1102 74-75 0.05783241710279441 1102 76-77 0.024476780985153823 1102 78-79 -0.052273449749201006 1102 80-81 0.015144825509384674 1102 82-83 -0.11901141449446939 1102 84-85 -0.11765522943023754 1102 86-87 -0.11168696148417467 1102 88-89 -0.364743143496419 1102 90-91 -0.08933399597794534 1102 92-93 -0.22004745376981205 1102 94-95 -0.3761758130210815 1102 96-97 -0.36238823560848843 1102 98-99 -0.6478359312672666 1102 100-101 -0.8430091129591766 1103 1 0.2440511468464166 1103 2 0.28340856033869244 1103 3 0.31894133545109327 1103 4 0.3052352650583643 1103 5 0.309941970736368 1103 6 0.2993628473429908 1103 7 0.33189954299432145 1103 8 0.39619243114982083 1103 9 0.4337102303372049 1103 10-11 0.44932100908657446 1103 12-13 0.4837808967687778 1103 14-15 0.5765733603505581 1103 16-17 0.5751031898736585 1103 18-19 0.6123152846070425 1103 20-21 0.5147360605579223 1103 22-23 0.5046971930786199 1103 24-25 0.518236573383156 1103 26-27 0.5282818478740552 1103 28-29 0.5443176885203656 1103 30-31 0.600786266916181 1103 32-33 0.4855655368249643 1103 34-35 0.5277290611038339 1103 36-37 0.45695426202016165 1103 38-39 0.5343314798647683 1103 40-41 0.4828605264831083 1103 42-43 0.5516723310174143 1103 44-45 0.5715321538081 1103 46-47 0.8238758061367193 1103 48-49 0.7311572654972665 1103 50-51 0.5486129140599374 1103 52-53 0.5116166185764186 1103 54-55 0.5000425978714631 1103 56-57 0.517356812722582 1103 58-59 0.6388385787695441 1103 60-61 0.672200678813553 1103 62-63 0.37252476961699443 1103 64-65 0.4929768309714362 1103 66-67 0.41258325433688015 1103 68-69 0.4940586970677998 1103 70-71 0.5149625872307553 1103 72-73 0.5736133919786965 1103 74-75 0.4731986035944189 1103 76-77 0.3959620181255197 1103 78-79 0.29976243101293676 1103 80-81 0.2968209479532895 1103 82-83 0.27887759969139125 1103 84-85 0.26273584337997846 1103 86-87 0.14035930381290385 1103 88-89 0.3051221061931422 1103 90-91 0.379883609757826 1103 92-93 0.2754009021827919 1103 94-95 0.4919182152862831 1103 96-97 0.4814415223733448 1103 98-99 0.21731722599701442 1103 100-101 0.2632874254804243 1104 1 0.27886836211929733 1104 2 0.25918885969664984 1104 3 0.3368468473288857 1104 4 0.18663343714042924 1104 5 0.32665694753761443 1104 6 0.3044766081703756 1104 7 0.27703345699144677 1104 8 0.22890416309287787 1104 9 0.3724372453361937 1104 10-11 0.36502931668669447 1104 12-13 0.39779286147670945 1104 14-15 0.5591373353583649 1104 16-17 0.3906440254811798 1104 18-19 0.4572195429181036 1104 20-21 0.5382655222025932 1104 22-23 0.3747386441633722 1104 24-25 0.3631397010816144 1104 26-27 0.40634245153639625 1104 28-29 0.5372173000136087 1104 30-31 0.45735237030222464 1104 32-33 0.39970681534996544 1104 34-35 0.43126378009713306 1104 36-37 0.42377431513069297 1104 38-39 0.18405525707190407 1104 40-41 0.24767473149992014 1104 42-43 0.33373855382441064 1104 44-45 0.2266418868280624 1104 46-47 0.5388534553387174 1104 48-49 0.6055593480856842 1104 50-51 0.4744252238873585 1104 52-53 0.5044715708716296 1104 54-55 0.4331715258669888 1104 56-57 0.512110487566872 1104 58-59 0.5220023437660686 1104 60-61 0.6266027896672881 1104 62-63 0.4898152282358623 1104 64-65 0.4137247029465172 1104 66-67 0.45342847205753856 1104 68-69 0.4352856378703365 1104 70-71 0.42950834241637637 1104 72-73 0.4361296268691177 1104 74-75 0.2630595523762551 1104 76-77 0.25175139064964824 1104 78-79 0.26063842966185646 1104 80-81 0.2516508543527003 1104 82-83 0.20788685799538698 1104 84-85 0.2997716032435136 1104 86-87 0.24526220538883692 1104 88-89 0.16799137487050686 1104 90-91 0.2684317759748396 1104 92-93 0.14554156452392064 1104 94-95 0.01127317252792892 1104 96-97 0.036127840971499126 1104 98-99 0.16398340273154588 1104 100-101 0.10479588882243007 1105 1 0.236275116234971 1105 2 0.2865222570735 1105 3 0.33835294639775526 1105 4 0.2929707048447625 1105 5 0.30439994089404365 1105 6 0.24844202682259464 1105 7 0.3098600661588762 1105 8 0.26458017069521844 1105 9 0.34791907191118554 1105 10-11 0.3943387795472404 1105 12-13 0.3074119828265296 1105 14-15 0.30878435600271104 1105 16-17 0.2599808494554168 1105 18-19 0.29690623556276563 1105 20-21 0.2832041332601136 1105 22-23 0.4097516129760592 1105 24-25 0.5018289024562321 1105 26-27 0.5654821210341652 1105 28-29 0.5086629048656306 1105 30-31 0.6275881177264253 1105 32-33 0.5445536127569 1105 34-35 0.4839009603739939 1105 36-37 0.552481607454105 1105 38-39 0.7361475292525554 1105 40-41 0.6721812802188722 1105 42-43 0.49080716892098053 1105 44-45 0.501755759165647 1105 46-47 0.43278635441393476 1105 48-49 0.6169508872909475 1105 50-51 0.5165972087225015 1105 52-53 0.6423100428901236 1105 54-55 0.593532327681082 1105 56-57 0.5889253073449154 1105 58-59 0.6155964310961295 1105 60-61 0.5434850650312839 1105 62-63 0.4743624418987693 1105 64-65 0.500106090715768 1105 66-67 0.49579595108126284 1105 68-69 0.39574172460140034 1105 70-71 0.44464562023851073 1105 72-73 0.5332096460175961 1105 74-75 0.35209766984122126 1105 76-77 0.500314515090782 1105 78-79 0.46307098013043557 1105 80-81 0.3129382152026814 1105 82-83 0.38033358683808416 1105 84-85 0.3529308189310534 1105 86-87 0.3010770744104221 1105 88-89 0.31731139683904885 1105 90-91 0.34456794971898574 1105 92-93 0.2187352345485678 1105 94-95 0.2793629776876294 1105 96-97 0.15955463747967258 1105 98-99 0.2360388202885666 1105 100-101 0.0097477714193559 1106 1 0.21782740166239023 1106 2 0.3293747525897075 1106 3 0.3412404645129996 1106 4 0.32107849893330354 1106 5 0.3532973973348774 1106 6 0.2759082946872269 1106 7 0.4005136765836852 1106 8 0.30946464266608587 1106 9 0.36527200520988146 1106 10-11 0.3437693762654419 1106 12-13 0.31683789356882386 1106 14-15 0.40573373888152986 1106 16-17 0.3852460608691217 1106 18-19 0.49086169070702823 1106 20-21 0.5350536244988717 1106 22-23 0.5219209259709103 1106 24-25 0.5403099441471184 1106 26-27 0.47235238061173845 1106 28-29 0.3000645551508825 1106 30-31 0.41481100609227184 1106 32-33 0.4331958941458538 1106 34-35 0.41254384506283515 1106 36-37 0.5538507341541035 1106 38-39 0.5174177719793462 1106 40-41 0.7005049100455025 1106 42-43 0.7519844046476649 1106 44-45 0.6801164321258781 1106 46-47 0.4802791882773647 1106 48-49 0.6640022980635436 1106 50-51 0.6921760710705271 1106 52-53 0.5641620331426722 1106 54-55 0.5572138040599484 1106 56-57 0.5815868922501437 1106 58-59 0.6949778250673475 1106 60-61 0.7295917705795887 1106 62-63 0.726829405378588 1106 64-65 0.6305143037076704 1106 66-67 0.6101821311449527 1106 68-69 0.7358559407133072 1106 70-71 0.5853195530282989 1106 72-73 0.5775923649081847 1106 74-75 0.5995022450907896 1106 76-77 0.5608464279649148 1106 78-79 0.694703423830461 1106 80-81 0.49131930154295844 1106 82-83 0.6465449961232324 1106 84-85 0.6207842701054993 1106 86-87 0.5118105076522959 1106 88-89 0.6229006063003517 1106 90-91 0.5737901843365947 1106 92-93 0.5616714379251242 1106 94-95 0.4892151250061829 1106 96-97 0.5127793144216817 1106 98-99 0.5103782654138911 1106 100-101 0.3596875844535994 1107 1 0.15678291346558382 1107 2 0.1692372845052219 1107 3 0.12927633533033145 1107 4 0.168702485630142 1107 5 0.150676539084877 1107 6 0.0898753437536115 1107 7 0.21653780055483196 1107 8 0.1259691247334871 1107 9 0.19695417132913207 1107 10-11 0.21716888377090982 1107 12-13 0.13925876069168197 1107 14-15 0.15508739717558484 1107 16-17 0.15317764502586328 1107 18-19 0.23029559735947913 1107 20-21 0.14923785457713734 1107 22-23 0.17430943266405308 1107 24-25 0.21785656871361425 1107 26-27 0.18238232905228813 1107 28-29 0.23098974932111105 1107 30-31 0.16567904172852366 1107 32-33 0.15976721839929553 1107 34-35 0.0922734983024256 1107 36-37 0.06622237732045022 1107 38-39 0.16303766594361235 1107 40-41 0.26569631132962 1107 42-43 0.253500566759854 1107 44-45 0.2795032616451536 1107 46-47 0.23351319151136352 1107 48-49 0.2424657589773389 1107 50-51 0.14701399610712684 1107 52-53 0.36787596102878695 1107 54-55 0.35893057431976416 1107 56-57 0.275886387874138 1107 58-59 0.34516367061743125 1107 60-61 0.1552557177667211 1107 62-63 0.2138661109583353 1107 64-65 0.29798666654426853 1107 66-67 0.24392693045267322 1107 68-69 0.3570935818184964 1107 70-71 0.27992768637815857 1107 72-73 0.3374273399749512 1107 74-75 0.21947155214618874 1107 76-77 0.25827768499882 1107 78-79 0.30223326645964477 1107 80-81 0.29577365401055644 1107 82-83 0.20614025108272926 1107 84-85 0.23669666747484897 1107 86-87 0.316857786209507 1107 88-89 0.1677234474808067 1107 90-91 0.2175117204422392 1107 92-93 0.27994230685824206 1107 94-95 0.2715615315247746 1107 96-97 0.22741269461797486 1107 98-99 0.24775114517087715 1107 100-101 0.06841171867442242 1108 1 -0.041771400771210665 1108 2 0.02500011413058445 1108 3 0.15569287771976548 1108 4 0.06823561023586677 1108 5 0.08398254596301058 1108 6 0.12151104528282985 1108 7 -0.02908294119369259 1108 8 0.06753024054435741 1108 9 0.25181881331865696 1108 10-11 0.10680876566635789 1108 12-13 0.1719240494073091 1108 14-15 0.19901249361762297 1108 16-17 0.16551452207716721 1108 18-19 0.17367266271137538 1108 20-21 0.14516190059799783 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88-89 0.46926724684648846 1108 90-91 0.4116138574291739 1108 92-93 0.4996722013219248 1108 94-95 0.5881184078589996 1108 96-97 0.751781690913397 1108 98-99 0.7722841486487013 1108 100-101 0.670227958922414 1109 1 0.07358676767950811 1109 2 0.04803910791135735 1109 3 0.1496470657730029 1109 4 0.20065962262324888 1109 5 0.08068280434294195 1109 6 0.21887988786607337 1109 7 0.23624887853929266 1109 8 0.2561431836845216 1109 9 0.10307972133957577 1109 10-11 0.14892690078072945 1109 12-13 0.13335753572571463 1109 14-15 0.09035107812271548 1109 16-17 0.10408718766203151 1109 18-19 0.07155304185432954 1109 20-21 0.13967289393304583 1109 22-23 0.22242949517259802 1109 24-25 0.18466150314444718 1109 26-27 0.22033539002693203 1109 28-29 0.15886690705438866 1109 30-31 0.1717289765554355 1109 32-33 0.345633300308549 1109 34-35 0.19621196914737027 1109 36-37 0.18996806545462874 1109 38-39 0.12197718099823618 1109 40-41 0.1932256392186602 1109 42-43 0.25819987038002523 1109 44-45 0.15275961152021722 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-1.21880308159092 2316 80-81 -1.1636326170070923 2316 82-83 -1.0969513932411417 2316 84-85 -1.0065005946797605 2316 86-87 -0.9955809740965478 2316 88-89 -1.4064081941863797 2316 90-91 -1.4161698101587241 2316 92-93 -1.3796904392673923 2316 94-95 -1.271787595947579 2316 96-97 -1.3358926814836103 2316 98-99 -1.6485573032702732 2316 100-101 -1.4592120589493547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7811.0 3 2351.0 4 2428.0 5 2500.0 6 2848.0 7 3324.0 8 3967.0 9 4958.0 10 5881.0 11 7024.0 12 8665.0 13 12745.0 14 11047.0 15 10624.0 16 12241.0 17 15652.0 18 14076.0 19 11336.0 20 11395.0 21 12170.0 22 13211.0 23 14664.0 24 16290.0 25 18359.0 26 21057.0 27 24411.0 28 28647.0 29 34025.0 30 40429.0 31 49432.0 32 62268.0 33 80488.0 34 112397.0 35 166610.0 36 271972.0 37 448216.0 38 450369.0 39 82170.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96747986928793 18.766129688195246 14.529230798673742 23.73715964384309 2 15.19917963243792 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22.933756170348364 26.425858506064927 28.664213792195454 21.97617153139126 22-23 22.607449863568412 26.25709917749846 28.532867262644938 22.602583696288185 24-25 22.524191008769836 26.412549741867146 28.657065015125344 22.406194234237674 26-27 22.34261685195055 26.610213890398526 28.502791067629296 22.54437819002163 28-29 22.27792260832392 26.96544646401417 28.44907955204739 22.307551375614523 30-31 22.498202651250416 26.64326813580944 28.379099135889106 22.479430077051042 32-33 22.263864257354772 26.56312875013865 28.41020956052043 22.762797431986158 34-35 22.712698099559415 26.776807331551854 28.215642317832103 22.29485225105663 36-37 22.684079677678334 26.44711853256147 28.317090192615325 22.551711597144873 38-39 22.348453635905464 26.78623784821697 28.373730686018817 22.49157782985875 40-41 22.763583611135523 26.63340144585653 28.279764067095904 22.32325087591204 42-43 22.509955101438557 26.727387355967213 28.07776955904362 22.68488798355061 44-45 22.557239345195402 26.81877788781582 28.126489765743546 22.497493001245232 46-47 22.784589372698182 26.92166908057602 27.70146640801185 22.592275138713948 48-49 22.662418322183424 26.964657686848298 27.760311917923904 22.612612073044374 50-51 22.64427710460933 27.13947822903847 27.696326511838055 22.519918154514137 52-53 22.825583615477733 27.057761564207105 27.62308047781163 22.493574342503535 54-55 22.681380448288525 27.03098024457546 27.678986148996565 22.608653158139454 56-57 22.63677308744468 27.225248625545635 27.580546007490558 22.557432279519126 58-59 22.65036756782644 26.967170326381073 27.699671942721675 22.682790163070806 60-61 22.519799126016764 27.038386766422366 27.857683228822154 22.584130878738716 62-63 22.50021016952238 27.067846553974288 27.901602821037834 22.5303404554655 64-65 22.376627542790416 27.040587138449933 27.81152499265095 22.7712603261087 66-67 22.37425001419645 27.13740413204111 27.60213715853947 22.886208695222972 68-69 22.548366232508272 26.931935361035404 27.841182908485006 22.678515497971315 70-71 22.509114296901547 26.856754931688904 27.617218221213225 23.016912550196327 72-73 22.487678768667536 26.991774664625307 27.608614918693053 22.911931648014107 74-75 22.44672044240992 27.207203537554165 27.434758075047235 22.911317944988685 76-77 22.544016825805958 27.050237870519865 27.46731376391171 22.938431539762465 78-79 22.514110384783393 27.11665351102392 27.559794312164243 22.809441792028448 80-81 22.61932960586113 27.055652221813737 27.439288866322343 22.885729306002794 82-83 22.66620771823824 27.142152568733874 27.423869294553626 22.767770418474257 84-85 22.59355538113053 27.14010846021796 27.407247016577536 22.859089142073977 86-87 22.60589221556886 27.091736526946107 27.42768862275449 22.87468263473054 88-89 22.65986995052179 27.09673942075402 27.264349698711122 22.979040930013067 90-91 22.55361296788481 27.14396166331677 27.38364979082395 22.918775577974472 92-93 22.64236659713247 26.918480138665853 27.462437875675157 22.97671538852652 94-95 22.579730847070543 26.828656456371125 27.499130879798823 23.092481816759513 96-97 22.710135003356754 26.63035014234461 27.555673969744642 23.103840884553993 98-99 22.485752792835964 26.766947198179043 27.39922729042499 23.348072718560005 100-101 22.879671425245217 26.19411608580964 27.801082537495486 23.12512995144966 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1654.0 1 895.5 2 166.0 3 206.0 4 201.0 5 204.0 6 343.5 7 697.5 8 1141.5 9 1376.5 10 1384.0 11 1426.5 12 1603.0 13 1816.5 14 1981.0 15 2150.0 16 2383.5 17 2568.5 18 2599.5 19 2760.5 20 3068.5 21 3463.5 22 4144.5 23 5283.0 24 6706.0 25 8404.5 26 10906.0 27 13869.0 28 16608.0 29 19609.5 30 22897.5 31 27174.5 32 32607.0 33 36589.5 34 39779.5 35 43819.5 36 48296.5 37 52450.0 38 56053.0 39 59044.5 40 62185.5 41 65919.5 42 68286.5 43 70331.0 44 73812.5 45 77242.0 46 78616.5 47 80124.5 48 82855.0 49 82564.5 50 79333.0 51 76931.0 52 75905.5 53 74542.0 54 72293.0 55 66821.5 56 60626.5 57 54999.5 58 49876.0 59 44667.5 60 36878.5 61 30060.0 62 24152.0 63 18002.5 64 13149.5 65 9844.5 66 7334.5 67 5734.0 68 4508.0 69 3482.0 70 2662.0 71 1857.0 72 1334.0 73 1008.0 74 767.0 75 592.0 76 417.5 77 260.5 78 167.5 79 118.5 80 87.0 81 55.0 82 43.0 83 29.0 84 18.5 85 15.0 86 11.0 87 13.5 88 12.0 89 6.5 90 4.0 91 4.0 92 3.5 93 2.5 94 4.0 95 3.5 96 2.5 97 3.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38115673944884076 2 0.1163440791540103 3 0.11744031586869372 4 0.12883164520823015 5 0.129832556991202 6 0.07406747193991478 7 0.08283736565738217 8 0.07988229277432252 9 0.0794533305816203 10-11 0.07792813167423467 12-13 0.08164580401098716 14-15 0.07583098317657942 16-17 0.07735618208396505 18-19 0.08598308840386505 20-21 0.0844102270306236 22-23 0.09444317609326973 24-25 0.10805081009510092 26-27 0.10933769667320756 28-29 0.102521964055828 30-31 0.09265583362367719 32-33 0.09124979088093105 34-35 0.3395712379896544 36-37 0.5292201896298867 38-39 0.5118472208254472 40-41 0.4823679856936342 42-43 0.510703321644908 44-45 0.5209507518039053 46-47 0.5184723035794034 48-49 0.5242632931808834 50-51 0.5235006937271905 52-53 0.5217371824905259 54-55 0.5165419737122436 56-57 0.4889454059817348 58-59 0.47838816979467486 60-61 0.42498237680324985 62-63 0.4998124481968574 64-65 0.5282669403127705 66-67 0.5384667080059121 68-69 0.5546242839310286 70-71 0.5495482313173858 72-73 0.535821441150915 74-75 0.5402302192425766 76-77 0.5155648931621997 78-79 0.48915988707808594 80-81 0.4973339999723558 82-83 0.5102981906851336 84-85 0.5049123320434281 86-87 0.5046025260153655 88-89 0.5149691123390022 90-91 0.5342247485447458 92-93 0.5406830126682068 94-95 0.5341294236130342 96-97 0.5362027408777615 98-99 0.5373704712912286 100-101 0.5132770948011212 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2098087.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.15789135200313 #Duplication Level Percentage of deduplicated Percentage of total 1 80.22917680913581 51.473348089811836 2 11.99502561267674 15.39151100045218 3 3.52054498309425 6.776122275756016 4 1.489680587762238 3.822990611953513 5 0.7633843921344157 2.4488566445187394 6 0.4420969979798202 1.701840669806163 7 0.3054612160320114 1.3718423257302776 8 0.20752745058648867 1.065161890182891 9 0.16224522093704083 0.9368380131534367 >10 0.8150670056558241 9.418637951327744 >50 0.04901914831910423 2.1242875088204607 >100 0.01928285191846707 2.3389215015114106 >500 8.926342606610705E-4 0.3888123970764035 >1k 5.950895071073193E-4 0.7408291198989259 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4394 0.20942887497038967 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3516 0.16758122994899638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2226 0.10609664899501309 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.766246585580102E-5 5 0.0 0.0 0.0 0.0 4.766246585580102E-5 6 4.766246585580102E-5 0.0 0.0 0.0 4.766246585580102E-5 7 9.532493171160205E-5 0.0 0.0 0.0 4.766246585580102E-5 8 9.532493171160205E-5 0.0 0.0 0.0 4.766246585580102E-5 9 9.532493171160205E-5 0.0 0.0 0.0 4.766246585580102E-5 10-11 9.532493171160205E-5 0.0 0.0 0.0 4.766246585580102E-5 12-13 9.532493171160205E-5 0.0 0.0 0.0 7.149369878370153E-5 14-15 1.4298739756740306E-4 0.0 0.0 0.0 1.668186304953036E-4 16-17 1.4298739756740306E-4 0.0 0.0 4.766246585580102E-5 1.906498634232041E-4 18-19 1.668186304953036E-4 0.0 0.0 9.532493171160205E-5 2.3831232927900512E-4 20-21 1.906498634232041E-4 0.0 0.0 1.668186304953036E-4 2.3831232927900512E-4 22-23 1.906498634232041E-4 0.0 0.0 1.906498634232041E-4 2.6214356220690565E-4 24-25 3.336372609906072E-4 0.0 0.0 3.336372609906072E-4 2.859747951348061E-4 26-27 3.336372609906072E-4 0.0 0.0 6.196120561254133E-4 3.0980602806270665E-4 28-29 3.336372609906072E-4 0.0 0.0 0.002049486031799444 3.336372609906072E-4 30-31 3.336372609906072E-4 0.0 0.0 0.00605313316368673 3.5746849391850766E-4 32-33 3.336372609906072E-4 0.0 0.0 0.012749709616426772 3.812997268464082E-4 34-35 4.289621927022092E-4 0.0 0.0 0.024832144710872334 4.289621927022092E-4 36-37 5.719495902696122E-4 0.0 0.0 0.04525551133008307 4.289621927022092E-4 38-39 6.672745219812144E-4 0.0 0.0 0.07533052728509351 4.527934256301097E-4 40-41 6.672745219812144E-4 0.0 0.0 0.10826529119145202 4.7662465855801024E-4 42-43 6.911057549091148E-4 0.0 0.0 0.1436785033223122 5.242871244138113E-4 44-45 7.149369878370153E-4 0.0 0.0 0.1910311631500505 5.242871244138113E-4 46-47 7.387682207649158E-4 0.0 0.0 0.24767800381967 5.242871244138113E-4 48-49 7.625994536928164E-4 0.0 0.0 0.307327579838205 5.719495902696122E-4 50-51 7.625994536928164E-4 0.0 0.0 0.3714335964142573 5.719495902696122E-4 52-53 7.86430686620717E-4 0.0 0.0 0.4365881872391374 5.719495902696122E-4 54-55 8.102619195486174E-4 0.0 0.0 0.51499294357193 5.719495902696122E-4 56-57 8.102619195486174E-4 0.0 0.0 0.6082207267858768 5.719495902696122E-4 58-59 8.102619195486174E-4 0.0 0.0 0.7091459982355355 5.719495902696122E-4 60-61 8.340931524765179E-4 0.0 0.0 0.8232261102613952 5.719495902696122E-4 62-63 8.579243854044184E-4 0.0 0.0 0.9434785116155813 6.196120561254133E-4 64-65 8.579243854044184E-4 0.0 0.0 1.0731204187433598 6.196120561254133E-4 66-67 8.579243854044184E-4 0.0 0.0 1.2275229768832274 6.196120561254133E-4 68-69 9.055868512602194E-4 0.0 0.0 1.4025395515057286 6.196120561254133E-4 70-71 9.055868512602194E-4 0.0 0.0 1.5958585130168577 6.196120561254133E-4 72-73 9.2941808418812E-4 0.0 0.0 1.7925615096037486 6.196120561254133E-4 74-75 9.532493171160205E-4 0.0 0.0 2.0009179790923826 6.434432890533139E-4 76-77 9.532493171160205E-4 0.0 0.0 2.2361799105566167 7.149369878370153E-4 78-79 9.77080550043921E-4 0.0 0.0 2.502493938525905 7.149369878370153E-4 80-81 0.0010009117829718214 0.0 0.0 2.7933064739450746 8.102619195486174E-4 82-83 0.0010009117829718214 0.0 0.0 3.097631318434364 9.055868512602194E-4 84-85 0.0010009117829718214 0.0 0.0 3.408867220472745 9.055868512602194E-4 86-87 0.001024743015899722 0.0 0.0 3.743481562013396 9.055868512602194E-4 88-89 0.0010485742488276226 0.0 0.0 4.117107631857021 9.055868512602194E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4015 0.0 35.004215 1 GTATCAA 6250 0.0 33.35022 1 TCAACGC 7300 0.0 28.224907 4 ATCAACG 7270 0.0 28.211073 3 TATCAAC 7415 0.0 27.787163 2 CAACGCA 7390 0.0 27.752975 5 AACGCAG 7685 0.0 26.85908 6 GTACATG 5720 0.0 24.321247 1 TACATGG 5525 0.0 24.261646 2 ACGCAGA 8690 0.0 23.425947 7 CGCAGAG 8725 0.0 23.169195 8 ACATGGG 5740 0.0 22.940296 3 CATGGGG 3900 0.0 20.160885 4 GCAGAGT 9910 0.0 19.92099 9 GTACCGT 120 0.0048573795 19.72592 6 GTACTTT 5265 0.0 19.423603 14-15 GAGTACT 5365 0.0 19.370197 12-13 AGAGTAC 7945 0.0 19.187607 10-11 GTATAGC 930 0.0 18.890045 1 GTGTAGC 1380 0.0 18.579279 1 >>END_MODULE