FastQCFastQC Report
Fri 27 May 2016
SRR939100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098087
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43530.20747471387030184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38810.18497802998636376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.11105354544401638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38550.039.922511
GTATCAA60700.033.9890371
ATCAACG74350.027.7635173
TCAACGC75200.027.4497034
TATCAAC75800.027.3576282
CAACGCA75900.027.07155
AACGCAG79200.025.8836066
ACGCAGA90100.022.646967
CGCAGAG90800.022.5246288
TACATGG58000.021.272162
GTACATG61350.020.7365461
ACATGGG56650.020.690133
GAGTACT56600.019.95480312-13
GTACTTT58650.019.05503714-15
GGACCGT2001.2965153E-518.981316
GCAGAGT105350.018.9187019
AGAGTAC85650.018.81045510-11
GTATACG1559.4297586E-418.4441741
AGTACTT59750.018.30711612-13
CATGGGG39050.017.7418164
Produced by FastQC (version 0.11.5)