##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939100_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2098087 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5193130694771 34.0 31.0 34.0 31.0 34.0 2 32.742588844027914 34.0 31.0 34.0 31.0 34.0 3 32.85293412522932 34.0 31.0 34.0 31.0 34.0 4 36.28651004462637 37.0 37.0 37.0 35.0 37.0 5 36.2465793839817 37.0 37.0 37.0 35.0 37.0 6 36.278891199459316 37.0 37.0 37.0 35.0 37.0 7 36.239194561522 37.0 37.0 37.0 35.0 37.0 8 36.21099887659568 37.0 37.0 37.0 35.0 37.0 9 37.96977580052686 39.0 38.0 39.0 35.0 39.0 10-11 37.8822877697636 39.0 38.0 39.0 35.0 39.0 12-13 37.79766973438184 39.0 38.0 39.0 35.0 39.0 14-15 39.23743128859766 40.5 39.0 41.0 36.0 41.0 16-17 39.331109482113945 41.0 39.0 41.0 36.0 41.0 18-19 39.34975909006633 41.0 39.0 41.0 36.0 41.0 20-21 39.3062067969536 41.0 39.0 41.0 36.0 41.0 22-23 39.24125786966889 41.0 39.0 41.0 36.0 41.0 24-25 39.168790188395434 41.0 39.0 41.0 36.0 41.0 26-27 39.0301953636813 40.0 39.0 41.0 35.0 41.0 28-29 38.88245577995574 40.0 38.0 41.0 35.0 41.0 30-31 38.70529796905467 40.0 38.0 41.0 35.0 41.0 32-33 38.62618185041897 40.0 38.0 41.0 35.0 41.0 34-35 38.531906207893186 40.0 38.0 41.0 34.0 41.0 36-37 38.39273085434493 40.0 38.0 41.0 34.0 41.0 38-39 38.265076948667996 40.0 38.0 41.0 34.0 41.0 40-41 38.085711888973144 40.0 38.0 41.0 33.0 41.0 42-43 37.910095243905516 40.0 38.0 41.0 33.0 41.0 44-45 37.67351997319463 40.0 37.0 41.0 33.0 41.0 46-47 37.408218296000115 40.0 37.0 41.0 32.5 41.0 48-49 37.093224685153665 40.0 37.0 41.0 31.5 41.0 50-51 36.83255675288966 40.0 36.0 41.0 31.0 41.0 52-53 36.55653173581457 39.0 36.0 41.0 30.5 41.0 54-55 36.29173790219376 39.0 35.0 40.0 30.0 41.0 56-57 36.019896696371504 39.0 35.0 40.0 29.5 41.0 58-59 35.65791313706248 38.0 35.0 40.0 28.5 41.0 60-61 35.26672178036468 38.0 34.5 40.0 28.0 41.0 62-63 34.836794661041225 37.5 34.0 40.0 27.0 41.0 64-65 34.35926417731962 37.0 34.0 39.5 26.0 41.0 66-67 33.91618698366655 36.0 33.0 39.0 26.0 40.5 68-69 33.4007665077759 36.0 33.0 39.0 25.5 40.0 70-71 32.87008665512917 35.0 32.0 38.0 23.5 40.0 72-73 32.26484888376888 35.0 31.5 37.5 22.0 39.5 74-75 31.69240860841328 35.0 31.0 37.0 20.5 39.0 76-77 29.687552994704223 32.5 28.5 35.0 18.0 37.0 78-79 30.673408919649184 34.0 30.0 36.0 18.0 38.0 80-81 30.674394102818425 34.0 31.0 35.5 18.0 37.0 82-83 30.257197151500392 34.0 30.0 35.0 14.5 37.0 84-85 29.744758201161343 34.0 30.0 35.0 7.5 36.0 86-87 28.999567939747017 34.0 29.0 35.0 2.0 36.0 88-89 28.279304671350616 33.0 28.0 35.0 2.0 35.5 90-91 27.674707006906768 33.0 26.5 35.0 2.0 35.0 92-93 27.171004824871417 33.0 26.0 34.5 2.0 35.0 94-95 26.575925831483634 32.0 25.0 34.0 2.0 35.0 96-97 25.695951359500345 32.0 23.5 34.0 2.0 35.0 98-99 24.390922778702695 32.0 10.0 34.0 2.0 35.0 100-101 18.441224076980603 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1372617597861776 1101 2 0.17108006458079927 1101 3 0.1553943986549129 1101 4 0.10379223983257191 1101 5 0.16975676445470356 1101 6 0.10539108888964677 1101 7 0.16517407012309349 1101 8 0.15693728487346448 1101 9 0.2297944547662496 1101 10-11 0.21705551386454403 1101 12-13 0.20738104102670718 1101 14-15 0.2076754364483051 1101 16-17 0.19521028992535605 1101 18-19 0.17903780453531226 1101 20-21 0.18869211184469492 1101 22-23 0.18906820019380888 1101 24-25 0.2027972286681461 1101 26-27 0.15493848318792658 1101 28-29 0.213210252928981 1101 30-31 0.27893631747033254 1101 32-33 0.33687480766649003 1101 34-35 0.305318203304374 1101 36-37 0.3200187771517733 1101 38-39 0.21492689173565083 1101 40-41 0.27954930936805766 1101 42-43 0.35278049007803247 1101 44-45 0.2803695582551953 1101 46-47 0.2514536399947005 1101 48-49 0.2823274488549501 1101 50-51 0.20552566235664216 1101 52-53 0.23241415964015033 1101 54-55 0.14799950456127675 1101 56-57 0.14842727206892192 1101 58-59 0.12854105057655119 1101 60-61 0.11890440892687337 1101 62-63 0.1082326474296309 1101 64-65 0.09338598642845852 1101 66-67 0.22281133082256588 1101 68-69 0.2893650810136634 1101 70-71 0.1964731800859454 1101 72-73 0.24272956364109888 1101 74-75 0.11509121730612648 1101 76-77 0.33856694265626075 1101 78-79 0.24440549429372993 1101 80-81 0.36891953230203356 1101 82-83 0.4407263217827797 1101 84-85 0.5065169064660857 1101 86-87 0.6593850641100332 1101 88-89 0.39584059114390513 1101 90-91 0.37329413075154605 1101 92-93 0.38408219522904474 1101 94-95 0.3613094867822504 1101 96-97 0.19789237364193468 1101 98-99 -0.015728250592694337 1101 100-101 -0.6362050080092594 1102 1 0.0823089112163089 1102 2 0.10014638832797118 1102 3 0.07393932890197163 1102 4 0.09058983939613086 1102 5 0.04236217323032321 1102 6 0.1037076659296261 1102 7 0.11916050921591648 1102 8 0.09644706587260288 1102 9 0.1224840346898759 1102 10-11 0.16333990323403924 1102 12-13 0.17587141590006183 1102 14-15 0.20111515276036585 1102 16-17 0.14089067337169325 1102 18-19 0.17139225859189366 1102 20-21 0.20796184919954186 1102 22-23 0.11579838490271754 1102 24-25 0.1343204926382242 1102 26-27 0.13873553719773923 1102 28-29 0.22876048284091155 1102 30-31 0.2467233177041379 1102 32-33 0.16368098021735022 1102 34-35 0.12647399786442293 1102 36-37 0.17318298882662475 1102 38-39 0.1689990607014309 1102 40-41 0.27013461059166133 1102 42-43 0.27644583182848237 1102 44-45 0.376184381729594 1102 46-47 0.33977099639716357 1102 48-49 0.2905243937540263 1102 50-51 0.26304261349061875 1102 52-53 0.29554446904705856 1102 54-55 0.29870482760702544 1102 56-57 0.3416916318226484 1102 58-59 0.06812487101144171 1102 60-61 -0.02240569940450854 1102 62-63 -0.08589290786613901 1102 64-65 -0.03307336981812625 1102 66-67 -0.014329188232842682 1102 68-69 0.07207687276800101 1102 70-71 0.010864108306662956 1102 72-73 0.04140659582877504 1102 74-75 -0.0397856434217978 1102 76-77 0.001804414404368515 1102 78-79 0.08668980572697649 1102 80-81 0.18823018618715182 1102 82-83 -0.022875501925426533 1102 84-85 -0.08634803001587343 1102 86-87 0.05662417251935281 1102 88-89 -0.19581243667933634 1102 90-91 0.20817060179822988 1102 92-93 -0.13592209126460375 1102 94-95 0.02352404527340468 1102 96-97 -0.2427786574546289 1102 98-99 -0.4093959304305841 1102 100-101 -0.5990450048918099 1103 1 0.07816562803589022 1103 2 0.06190438771075435 1103 3 0.08887258266929621 1103 4 0.009422266642225452 1103 5 0.05871766950139801 1103 6 0.0439410108175835 1103 7 0.08984189559520672 1103 8 0.028311353164419018 1103 9 0.10950161088091193 1103 10-11 0.11675721966545183 1103 12-13 0.14443728237249331 1103 14-15 0.06968306688757764 1103 16-17 0.14992039215556474 1103 18-19 0.1904157027764839 1103 20-21 0.1344732743818895 1103 22-23 0.10564230973038491 1103 24-25 0.15648650186144408 1103 26-27 0.16306386986558863 1103 28-29 0.12946765504428726 1103 30-31 0.05257668514554581 1103 32-33 0.15946120250490026 1103 34-35 0.12961334515983225 1103 36-37 0.22328749235078504 1103 38-39 0.3722229468869287 1103 40-41 0.17515370497245186 1103 42-43 0.012258143791612497 1103 44-45 0.0760505528637978 1103 46-47 0.2187193501558511 1103 48-49 0.06412266436330327 1103 50-51 0.008094675113326844 1103 52-53 0.031704882322294736 1103 54-55 -0.009994779974569212 1103 56-57 -0.13307775105808162 1103 58-59 -0.07718914416168587 1103 60-61 -0.03799244756783793 1103 62-63 -0.12604025125345686 1103 64-65 0.006357733180365699 1103 66-67 -0.0024118374300954315 1103 68-69 -0.18846613602666906 1103 70-71 -0.10070002204703599 1103 72-73 -0.2081108158185785 1103 74-75 -0.41074989868244316 1103 76-77 -0.23903079472700028 1103 78-79 -0.0556431166127922 1103 80-81 -0.10033848849754534 1103 82-83 -0.27233601802218743 1103 84-85 -0.37477673287044766 1103 86-87 -0.22983878174920136 1103 88-89 -0.05691255383444371 1103 90-91 0.10972579560144169 1103 92-93 -0.19878246022274126 1103 94-95 -0.44047364965236113 1103 96-97 -0.5417343535183043 1103 98-99 -0.5427308726355307 1103 100-101 -0.8730314523424063 1104 1 0.016175834641209974 1104 2 0.0476199453642181 1104 3 0.09855769292521899 1104 4 0.061198671039740304 1104 5 0.03920244792252703 1104 6 0.030998322983968762 1104 7 0.0778540101093057 1104 8 0.04629092016805458 1104 9 0.05056814601964987 1104 10-11 0.10522002463053326 1104 12-13 0.10905575008149526 1104 14-15 0.048114794138584216 1104 16-17 0.11621004662621459 1104 18-19 0.195882214737658 1104 20-21 0.2643074559102203 1104 22-23 0.1799090014589666 1104 24-25 0.14390363149041718 1104 26-27 0.16627622293894717 1104 28-29 0.17453684146958892 1104 30-31 0.09481665484513258 1104 32-33 0.10398684691875815 1104 34-35 0.18720661010052453 1104 36-37 0.2798723921714412 1104 38-39 0.17322947476147732 1104 40-41 0.2834299305918577 1104 42-43 0.20505939198793044 1104 44-45 0.41097658960023153 1104 46-47 0.3568913756907435 1104 48-49 0.2188436079725875 1104 50-51 0.09516922912198567 1104 52-53 0.04613927216600899 1104 54-55 -0.02441037338365959 1104 56-57 0.026515143606985703 1104 58-59 0.04834560027040169 1104 60-61 0.14014645101762113 1104 62-63 0.05182475642764217 1104 64-65 0.12959295289373074 1104 66-67 0.11611452209623963 1104 68-69 0.10590638104204686 1104 70-71 -0.028479173833197535 1104 72-73 -0.2525838867461445 1104 74-75 -0.41258304570023796 1104 76-77 -0.36341189031888277 1104 78-79 -0.006036499137195506 1104 80-81 0.04491908625361418 1104 82-83 -0.18602137701288868 1104 84-85 -0.3540544410056299 1104 86-87 -0.1869218577503986 1104 88-89 0.04619945736992648 1104 90-91 -0.04628333974843457 1104 92-93 -0.30591111404470794 1104 94-95 -0.20616040494963528 1104 96-97 -0.5346103634733481 1104 98-99 -0.7327166127840101 1104 100-101 -1.3987126111072676 1105 1 0.07634875752555104 1105 2 0.06207709715516785 1105 3 0.11339130221077909 1105 4 0.03610464412047776 1105 5 0.03603525370409244 1105 6 0.0684446764455302 1105 7 0.08802885699012819 1105 8 0.019028562661524973 1105 9 0.10530786427659677 1105 10-11 0.1626305998301305 1105 12-13 0.15697294988355281 1105 14-15 0.16994130993164447 1105 16-17 0.1563783842098303 1105 18-19 0.18082499131484298 1105 20-21 0.19716105484656765 1105 22-23 0.222221047856614 1105 24-25 0.08684395019572833 1105 26-27 0.15066628779781155 1105 28-29 0.170331092783357 1105 30-31 0.14085748716332347 1105 32-33 0.1744867873152245 1105 34-35 0.23816547344107875 1105 36-37 0.24795100316976004 1105 38-39 0.20166210649536254 1105 40-41 0.2661695293726609 1105 42-43 0.253020857163726 1105 44-45 0.14196972429639487 1105 46-47 0.05137566794122961 1105 48-49 -0.07654972552757755 1105 50-51 -0.048152767432036114 1105 52-53 -0.061759392688067294 1105 54-55 -0.12709150958027493 1105 56-57 -0.17718051728311934 1105 58-59 -0.31396325471488495 1105 60-61 -0.3492103502292352 1105 62-63 -0.3140262702733949 1105 64-65 -0.22303133795210783 1105 66-67 -0.1977062577654678 1105 68-69 -0.28149959540231606 1105 70-71 -0.3145323789288028 1105 72-73 -0.5406361645561688 1105 74-75 -0.622750331140324 1105 76-77 -0.5886413773545094 1105 78-79 -0.5290231444488427 1105 80-81 -0.3719528291292882 1105 82-83 -0.32118979935998837 1105 84-85 -0.43051056325663595 1105 86-87 -0.4493886149928983 1105 88-89 -0.5804620750515674 1105 90-91 -0.4018591488427745 1105 92-93 -0.33821851325083685 1105 94-95 -0.4418412292704552 1105 96-97 -0.9154209337755077 1105 98-99 -0.7420174539875646 1105 100-101 -1.2300760680365208 1106 1 0.025690549539469032 1106 2 0.08028245159775338 1106 3 0.07522192212482537 1106 4 0.06854007477908652 1106 5 0.07110310023812616 1106 6 0.09421286314188393 1106 7 0.11944231843107644 1106 8 0.18105125037815384 1106 9 0.12093047106782961 1106 10-11 0.1365318984071564 1106 12-13 0.12978115746656727 1106 14-15 0.19276203858788676 1106 16-17 0.07587945519716754 1106 18-19 -0.04442482554983229 1106 20-21 0.08173119196987244 1106 22-23 0.15807518658490238 1106 24-25 0.22001759108718488 1106 26-27 0.20246777508807412 1106 28-29 0.14394855436264464 1106 30-31 0.15157477173129763 1106 32-33 0.03422216229861874 1106 34-35 0.08293628059131919 1106 36-37 -9.949773056874278E-4 1106 38-39 0.0725209533032185 1106 40-41 0.06720668510490668 1106 42-43 0.01318002431862908 1106 44-45 0.13208340732003165 1106 46-47 0.09467465738790537 1106 48-49 0.06545276724516924 1106 50-51 0.05280751957227636 1106 52-53 0.1500893652292703 1106 54-55 -0.06587139275332987 1106 56-57 -0.10257156353255681 1106 58-59 -0.06334454726930971 1106 60-61 -0.13465428403579693 1106 62-63 -0.09969072059307393 1106 64-65 -0.12811172116195735 1106 66-67 -0.07569314483422573 1106 68-69 -0.2828112525865194 1106 70-71 -0.134110474669896 1106 72-73 -0.2472220410456032 1106 74-75 -0.34964752077698336 1106 76-77 -0.24785325965798322 1106 78-79 -0.2466904958088847 1106 80-81 -0.28377495863749047 1106 82-83 -0.47603555872082026 1106 84-85 -0.5413362337711582 1106 86-87 -0.4131915314798107 1106 88-89 -0.5223968006034241 1106 90-91 -0.4228779091291024 1106 92-93 -0.24548806894386743 1106 94-95 -0.5787148928116643 1106 96-97 -0.8794127715440077 1106 98-99 -0.923533359391179 1106 100-101 -1.1156970391569203 1107 1 0.05041777294218974 1107 2 0.046154589655323264 1107 3 -0.019383126122612282 1107 4 -0.011422975382643585 1107 5 0.058894253592193024 1107 6 0.05252063601919588 1107 7 0.09002089496991772 1107 8 0.05493583287201176 1107 9 0.038118163089961854 1107 10-11 0.022360544169579555 1107 12-13 0.028441662087331565 1107 14-15 0.08855824233111065 1107 16-17 0.10973717945223882 1107 18-19 0.07841630391381216 1107 20-21 0.03431520746779171 1107 22-23 0.01666365278926918 1107 24-25 0.06780921868013934 1107 26-27 0.06085978910922307 1107 28-29 0.05355581827454614 1107 30-31 0.11820773174174093 1107 32-33 -0.10156913077744889 1107 34-35 -0.08558093759477003 1107 36-37 -8.674937344963496E-4 1107 38-39 -0.11542488861613975 1107 40-41 0.05433288415162707 1107 42-43 -0.010429423131881776 1107 44-45 -0.09837897049333577 1107 46-47 0.1549593435004013 1107 48-49 -0.008696267168765814 1107 50-51 -0.005770798939813915 1107 52-53 -0.0038883516623613446 1107 54-55 -0.2347205781588073 1107 56-57 -0.22925288061122728 1107 58-59 -0.18532006328455708 1107 60-61 -0.2400050499825852 1107 62-63 -0.06529170109472204 1107 64-65 0.015677508719988964 1107 66-67 -0.16499130698007036 1107 68-69 -0.24976993312135676 1107 70-71 -0.35969507608231055 1107 72-73 -0.5371445412330011 1107 74-75 -0.572145744930836 1107 76-77 -0.49839914430982546 1107 78-79 -0.17572727583904424 1107 80-81 -0.19062985224735485 1107 82-83 -0.2759604199039636 1107 84-85 -0.28042058047140017 1107 86-87 -0.2711303414053745 1107 88-89 -0.508101812798504 1107 90-91 -0.0776766202192043 1107 92-93 -0.048846062699215764 1107 94-95 -0.17665567618291078 1107 96-97 -0.28720811145855407 1107 98-99 -0.45570779057223376 1107 100-101 -1.249843921648175 1108 1 -0.07918133245556191 1108 2 -0.013567765807593446 1108 3 -0.003759564516471414 1108 4 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2316 92-93 -0.24202712199401688 2316 94-95 0.11198002477506463 2316 96-97 0.3279507039372298 2316 98-99 0.5349123410431602 2316 100-101 1.6779088402278113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 10.0 5 23.0 6 52.0 7 178.0 8 408.0 9 798.0 10 1415.0 11 2350.0 12 3363.0 13 4751.0 14 6194.0 15 8475.0 16 10598.0 17 15001.0 18 15296.0 19 12374.0 20 12282.0 21 13130.0 22 13882.0 23 15680.0 24 17559.0 25 20424.0 26 23739.0 27 28192.0 28 33868.0 29 40773.0 30 50133.0 31 60837.0 32 76844.0 33 100267.0 34 138095.0 35 205374.0 36 331018.0 37 501292.0 38 319240.0 39 14165.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.199043041132384 19.306264648855826 14.932511760964177 23.56218054904761 2 15.536200357754467 20.16293890577464 40.47410808036083 23.826752656110067 3 19.679164877338263 23.665462871654036 29.485526577305897 27.169845673701804 4 13.382619500525955 16.829092406558928 36.56016170921416 33.22812638370096 5 14.180727491281345 36.7749287803604 34.34152158609247 14.702822142265786 6 32.66671019838548 35.85351799043605 17.274498149981387 14.205273661197083 7 28.953470470957594 31.910020890458785 20.639277589537517 18.497231049046107 8 25.73210739116157 36.239345651538756 19.49552139639586 18.533025560903813 9 25.651033536740854 17.561616844296733 20.358688653044414 36.428660965918006 10-11 24.969460275002895 26.698749861183067 27.17740017453995 21.154389689274087 12-13 25.726697701286934 23.629620697330473 27.784572327077 22.859109274305595 14-15 22.477581734797667 25.399677607854095 25.81739563960333 26.305345017744912 16-17 22.36880926086482 28.494642362632046 26.65141587382086 22.485132502682273 18-19 22.326481218367018 27.27537037310655 28.496029954906543 21.90211845361989 20-21 22.855343939502987 26.46284448642978 28.76722938562605 21.914582188441184 22-23 22.541987057734023 26.304891074583658 28.599695818142905 22.553426049539414 24-25 22.507032002732014 26.522119083269445 28.681467613465887 22.28938130053265 26-27 22.292330736040096 26.71684101777035 28.585509892076686 22.40531835411287 28-29 22.226871961666024 27.052078167415882 28.510669126368242 22.210380744549855 30-31 22.463606132634155 26.73840503277509 28.393936953043415 22.404051881547332 32-33 22.252662664924124 26.69897706767025 28.465611037869753 22.582749229535874 34-35 22.635947033372634 26.845000134664147 28.297132511667517 22.2219203202957 36-37 22.65341757637953 26.585284165334727 28.375532329520464 22.38576592876528 38-39 22.364615999229606 26.840556896224587 28.36730666000517 22.42752044454064 40-41 22.706828059651976 26.736329914362063 28.311045987848814 22.245796038137147 42-43 22.517603384730958 26.74141471534283 28.132072575709334 22.60890932421688 44-45 22.53058681741951 26.892114336320223 28.216516321350277 22.360782524909993 46-47 22.73553392838247 26.976760480805805 27.78827995336462 22.499425637447104 48-49 22.66168799119358 27.058821566771822 27.74451749499348 22.53497294704112 50-51 22.64390606605118 27.194683685482374 27.715042872735417 22.44636737573103 52-53 22.78013256838253 27.133765187048965 27.62995528784078 22.456146956727725 54-55 22.633070029984456 27.09327592230446 27.66796610436078 22.6056879433503 56-57 22.604875398279862 27.3217481655891 27.542211111561254 22.531165324569784 58-59 22.638382104216827 27.04822101004333 27.652446297374457 22.660950588365385 60-61 22.501729593360267 27.17559966187433 27.780760725564118 22.541910019201286 62-63 22.519335747312873 27.102123536205163 27.856869474947533 22.52167124153443 64-65 22.34471552698164 27.129707723226833 27.73892251702015 22.786654232771387 66-67 22.37912210736938 27.205491583363006 27.561322506121165 22.85406380314645 68-69 22.551637421270033 26.987064786648578 27.774650451812544 22.68664734026885 70-71 22.47751340687823 26.969922789059638 27.558007105055637 22.9945566990065 72-73 22.468467704151447 27.039631816983757 27.539897058606243 22.95200342025855 74-75 22.396116080982342 27.3141914515461 27.32732246088937 22.962370006582187 76-77 22.45840265726843 27.02818875193063 27.51028975914032 23.00311883166061 78-79 22.391273155088985 27.19795476787826 27.527187372035943 22.883584704996814 80-81 22.468388970683208 27.146711685583156 27.427801881814855 22.95709746191877 82-83 22.504262216009156 27.15533245284871 27.44426232086658 22.89614301027555 84-85 22.4413477610795 27.15051854379728 27.398935315837715 23.00919837928551 86-87 22.40230438152649 27.08707538074947 27.43898787761528 23.071632360108758 88-89 22.406425020843365 27.12944518841348 27.22758369173071 23.236546099012443 90-91 22.318161357228856 27.11818126777022 27.358615413295695 23.205041961705234 92-93 22.346833091287444 26.957580882012994 27.38477956347854 23.31080646322102 94-95 22.184838943684863 26.835649253653095 27.517437321444405 23.46207448121764 96-97 22.32488452576085 26.681639035940837 27.489160363702748 23.50431607459557 98-99 22.060852692672313 26.767029712361236 27.365218630530823 23.80689896443563 100-101 22.489355156127157 25.705364639076222 28.17678912854434 23.62849107625228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 150.0 1 111.0 2 106.5 3 142.0 4 131.5 5 148.0 6 243.5 7 511.0 8 846.0 9 1066.5 10 1075.0 11 982.5 12 1086.0 13 1225.5 14 1305.0 15 1429.5 16 1627.0 17 1793.5 18 1974.5 19 2278.0 20 2632.5 21 3241.5 22 4066.0 23 5156.5 24 6638.0 25 8374.0 26 10951.5 27 14136.5 28 17026.0 29 20112.0 30 23506.0 31 27760.0 32 33065.0 33 37026.5 34 40099.5 35 43978.5 36 48508.5 37 52656.0 38 56245.5 39 59409.0 40 62568.0 41 66167.5 42 68627.5 43 70881.0 44 74389.0 45 77908.5 46 79503.5 47 80995.0 48 83694.5 49 83674.5 50 80656.0 51 78028.0 52 76274.0 53 74789.0 54 72738.0 55 67395.0 56 60753.0 57 54711.5 58 49527.5 59 44156.5 60 36453.5 61 29537.5 62 23822.0 63 17728.5 64 12715.5 65 9380.0 66 6947.5 67 5416.0 68 4230.0 69 3234.5 70 2460.0 71 1709.0 72 1207.0 73 962.0 74 731.5 75 508.5 76 357.5 77 218.0 78 121.5 79 70.0 80 34.0 81 20.5 82 15.0 83 10.5 84 5.5 85 2.5 86 2.0 87 0.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41752320089681694 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010009117829718214 16-17 0.002573773156213255 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.383123292790051E-5 26-27 0.003312541376978171 28-29 2.383123292790051E-5 30-31 0.0 32-33 4.766246585580102E-5 34-35 0.013131009343273181 36-37 0.0278587112927157 38-39 0.02197239675952427 40-41 0.008817556183323189 42-43 0.0092941808418812 44-45 0.0039798158989593855 46-47 0.005195208778282312 48-49 0.003455528774545574 50-51 8.102619195486174E-4 52-53 0.0 54-55 0.0 56-57 9.532493171160205E-5 58-59 0.0013583802768903292 60-61 0.0024069545257179516 62-63 0.0014298739756740306 64-65 0.00278825425256436 66-67 0.001906498634232041 68-69 0.004432609324589495 70-71 1.668186304953036E-4 72-73 0.0 74-75 0.0 76-77 0.0929179771858841 78-79 0.015728613732414336 80-81 2.859747951348061E-4 82-83 0.0 84-85 0.0 86-87 0.10740736680604761 88-89 0.4143298156844783 90-91 0.4166414452784846 92-93 0.0 94-95 4.766246585580102E-5 96-97 0.0 98-99 1.906498634232041E-4 100-101 2.6214356220690565E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2098087.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.751193521818685 #Duplication Level Percentage of deduplicated Percentage of total 1 79.16719730899689 49.678361189168676 2 12.579419482190534 15.787471726381488 3 3.7229431007503373 7.008573689577123 4 1.5998086147690846 4.0155959993299 5 0.8270137253208613 2.5948049161404785 6 0.4816988542686954 1.8136306814071943 7 0.3004457246895751 1.3197329468959007 8 0.21897104362265724 1.099255546723197 9 0.155905768473669 0.8804945743793156 >10 0.8702876000411027 9.876801816077089 >50 0.05411509790776149 2.2988089224842154 >100 0.020295030391220187 2.356028065913468 >500 0.0012910810327214281 0.5163157833710914 >1k 6.07567544809977E-4 0.754124142150914 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4353 0.20747471387030184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3881 0.18497802998636376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2330 0.11105354544401638 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.766246585580102E-5 2 0.0 0.0 0.0 0.0 4.766246585580102E-5 3 0.0 0.0 0.0 0.0 4.766246585580102E-5 4 0.0 0.0 0.0 0.0 4.766246585580102E-5 5 0.0 0.0 0.0 0.0 4.766246585580102E-5 6 0.0 0.0 0.0 0.0 4.766246585580102E-5 7 0.0 0.0 0.0 0.0 4.766246585580102E-5 8 0.0 0.0 0.0 0.0 4.766246585580102E-5 9 0.0 0.0 0.0 0.0 4.766246585580102E-5 10-11 0.0 0.0 0.0 0.0 9.532493171160205E-5 12-13 2.383123292790051E-5 0.0 0.0 0.0 2.144810963511046E-4 14-15 4.766246585580102E-5 0.0 0.0 0.0 2.3831232927900512E-4 16-17 4.766246585580102E-5 0.0 0.0 4.766246585580102E-5 2.859747951348061E-4 18-19 7.149369878370153E-5 0.0 0.0 9.532493171160205E-5 2.859747951348061E-4 20-21 9.532493171160205E-5 0.0 0.0 1.668186304953036E-4 2.859747951348061E-4 22-23 9.532493171160205E-5 0.0 0.0 1.906498634232041E-4 3.5746849391850766E-4 24-25 9.532493171160205E-5 0.0 0.0 3.336372609906072E-4 4.289621927022092E-4 26-27 9.532493171160205E-5 0.0 0.0 6.672745219812144E-4 4.527934256301097E-4 28-29 1.1915616463950256E-4 0.0 0.0 0.0020733172647273445 4.7662465855801024E-4 30-31 1.906498634232041E-4 0.0 0.0 0.006148458095398332 4.7662465855801024E-4 32-33 1.906498634232041E-4 0.0 0.0 0.01298802194570578 5.719495902696122E-4 34-35 2.6214356220690565E-4 0.0 0.0 0.02511811950600714 5.719495902696122E-4 36-37 4.289621927022092E-4 0.0 0.0 0.04561297982400158 5.957808231975127E-4 38-39 4.289621927022092E-4 0.0 0.0 0.07597397057414684 6.196120561254133E-4 40-41 4.289621927022092E-4 0.0 0.0 0.10943302160491915 6.196120561254133E-4 42-43 4.7662465855801024E-4 2.383123292790051E-5 0.0 0.1453228583943373 6.196120561254133E-4 44-45 5.242871244138113E-4 4.766246585580102E-5 0.0 0.19327129904527315 6.196120561254133E-4 46-47 6.196120561254133E-4 4.766246585580102E-5 0.0 0.2507522328673692 6.196120561254133E-4 48-49 7.149369878370153E-4 4.766246585580102E-5 0.0 0.3109975897091017 7.86430686620717E-4 50-51 7.149369878370153E-4 4.766246585580102E-5 0.0 0.37548490601200046 8.102619195486174E-4 52-53 7.149369878370153E-4 4.766246585580102E-5 0.0 0.4414259275235012 8.340931524765179E-4 54-55 7.149369878370153E-4 4.766246585580102E-5 0.0 0.5203311397477798 8.579243854044184E-4 56-57 7.625994536928164E-4 4.766246585580102E-5 0.0 0.6143453536483473 8.579243854044184E-4 58-59 7.625994536928164E-4 4.766246585580102E-5 0.0 0.7163668618126893 8.817556183323188E-4 60-61 7.625994536928164E-4 4.766246585580102E-5 0.0 0.8318530165812952 9.055868512602194E-4 62-63 7.625994536928164E-4 4.766246585580102E-5 0.0 0.9536306168428669 9.532493171160205E-4 64-65 7.625994536928164E-4 4.766246585580102E-5 0.0 1.0837968110950595 0.0010009117829718217 66-67 7.625994536928164E-4 4.766246585580102E-5 0.0 1.2382946941666386 0.001072405481755523 68-69 8.102619195486174E-4 4.766246585580102E-5 0.0 1.4145504929013906 0.0010962367146834236 70-71 8.102619195486174E-4 4.766246585580102E-5 0.0 1.6090610160589147 0.001263055345178727 72-73 8.102619195486174E-4 4.766246585580102E-5 0.0 1.806383624701931 0.0013345490439624287 74-75 8.102619195486174E-4 4.766246585580102E-5 0.0 2.0168372426882204 0.0013345490439624287 76-77 8.102619195486174E-4 4.766246585580102E-5 0.0 2.2526949549756514 0.0013345490439624287 78-79 8.340931524765179E-4 4.766246585580102E-5 0.0 2.5196285950010653 0.0013822115098182297 80-81 9.532493171160205E-4 4.766246585580102E-5 0.0 2.811680354532486 0.0014298739756740306 82-83 9.532493171160205E-4 4.766246585580102E-5 0.0 3.116148186419343 0.0015251989073856327 84-85 9.77080550043921E-4 4.766246585580102E-5 0.0 3.426621489004031 0.0015251989073856327 86-87 0.0010009117829718214 4.766246585580102E-5 0.0 3.760544724789773 0.0015251989073856327 88-89 0.0010009117829718214 4.766246585580102E-5 0.0 4.1331222203845694 0.0016205238390972349 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3855 0.0 39.92251 1 GTATCAA 6070 0.0 33.989037 1 ATCAACG 7435 0.0 27.763517 3 TCAACGC 7520 0.0 27.449703 4 TATCAAC 7580 0.0 27.357628 2 CAACGCA 7590 0.0 27.0715 5 AACGCAG 7920 0.0 25.883606 6 ACGCAGA 9010 0.0 22.64696 7 CGCAGAG 9080 0.0 22.524628 8 TACATGG 5800 0.0 21.27216 2 GTACATG 6135 0.0 20.736546 1 ACATGGG 5665 0.0 20.69013 3 GAGTACT 5660 0.0 19.954803 12-13 GTACTTT 5865 0.0 19.055037 14-15 GGACCGT 200 1.2965153E-5 18.98131 6 GCAGAGT 10535 0.0 18.918701 9 AGAGTAC 8565 0.0 18.810455 10-11 GTATACG 155 9.4297586E-4 18.444174 1 AGTACTT 5975 0.0 18.307116 12-13 CATGGGG 3905 0.0 17.741816 4 >>END_MODULE