FastQCFastQC Report
Fri 27 May 2016
SRR939099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022466
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82550.27312135190271786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66010.2183978248225125No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45250.14971218865654734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41320.1367095610008516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143800.027.4036241
GGTATCA103500.025.6883661
ATCAACG168650.023.1188353
TCAACGC169050.023.0080854
CAACGCA169250.022.9529085
TATCAAC171550.022.7832532
GTACATG103900.022.7106531
AACGCAG174100.022.3586586
TACATGG105050.022.3234652
ACATGGG104500.021.3075753
GAGTACT92300.020.26612-13
ACGCAGA193050.020.0906497
AGTACTT95400.019.97975512-13
AGAGTAC147150.019.96807510-11
CGCAGAG194900.019.875658
GTACTTT97250.019.0893314-15
GCAGAGT208800.018.1430639
TAAGGTG13750.017.9164985
GTATTAG9950.017.6549871
TACTTTT108900.017.28635814-15
Produced by FastQC (version 0.11.5)