FastQCFastQC Report
Fri 27 May 2016
SRR939099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022466
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86620.2865871774901686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73210.2422194327413443No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50580.16734679562979368No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45970.1520943494484305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA151500.025.0472741
GGTATCA112000.024.613611
GTACATG105150.023.771951
TACATGG105550.023.0620842
ACATGGG109700.021.4110533
TCAACGC175550.021.2452164
CAACGCA177600.021.1068575
ATCAACG176800.021.095013
TATCAAC178250.021.0032712
GAGTACT95200.020.7100312-13
AACGCAG182600.020.4769336
GTACTTT98300.020.03278214-15
AGAGTAC151650.019.27458210-11
TAAGGTG14500.018.9801435
AGTACTT98850.018.67323512-13
CATGGGG67750.018.489884
ACGCAGA202650.018.4041357
CGCAGAG205750.018.1268448
GCAGAGT221650.016.6341259
GTGTAGC17550.016.5488361
Produced by FastQC (version 0.11.5)