##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939099_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3022466 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.499403136379364 34.0 31.0 34.0 31.0 34.0 2 32.709715841303094 34.0 31.0 34.0 31.0 34.0 3 32.80361400260582 34.0 31.0 34.0 31.0 34.0 4 36.261909645964586 37.0 37.0 37.0 35.0 37.0 5 36.219264004954894 37.0 37.0 37.0 35.0 37.0 6 36.25392609875512 37.0 37.0 37.0 35.0 37.0 7 36.21214233675416 37.0 37.0 37.0 35.0 37.0 8 36.193703750513656 37.0 37.0 37.0 35.0 37.0 9 37.94330887427683 39.0 38.0 39.0 35.0 39.0 10-11 37.87852038699525 39.0 38.0 39.0 35.0 39.0 12-13 37.81085891454197 39.0 38.0 39.0 35.0 39.0 14-15 39.184633673298556 40.5 39.0 41.0 36.0 41.0 16-17 39.121407155613994 40.0 38.0 41.0 36.0 41.0 18-19 39.13952348843627 40.5 38.5 41.0 36.0 41.0 20-21 39.20910160776002 41.0 39.0 41.0 36.0 41.0 22-23 39.16999810750559 41.0 39.0 41.0 36.0 41.0 24-25 39.11147106369435 41.0 39.0 41.0 35.0 41.0 26-27 38.973219549864254 40.0 38.5 41.0 35.0 41.0 28-29 38.821593030326895 40.0 38.0 41.0 35.0 41.0 30-31 38.63477140851212 40.0 38.0 41.0 34.5 41.0 32-33 38.4982679705909 40.0 38.0 41.0 34.0 41.0 34-35 38.451865463499004 40.0 38.0 41.0 34.0 41.0 36-37 38.347150472494974 40.0 38.0 41.0 34.0 41.0 38-39 38.21033404511415 40.0 38.0 41.0 34.0 41.0 40-41 38.04505294683216 40.0 38.0 41.0 33.0 41.0 42-43 37.88026019151249 40.0 38.0 41.0 33.0 41.0 44-45 37.66569814184841 40.0 37.0 41.0 33.0 41.0 46-47 37.390562209798226 40.0 37.0 41.0 32.5 41.0 48-49 37.08205617532174 40.0 37.0 41.0 31.5 41.0 50-51 36.81750878256364 40.0 36.0 41.0 31.0 41.0 52-53 36.57052850222302 39.0 36.0 41.0 31.0 41.0 54-55 36.31181128257522 39.0 35.0 41.0 30.0 41.0 56-57 36.01286118685867 39.0 35.0 40.5 29.5 41.0 58-59 35.692454439520574 38.0 35.0 40.0 29.0 41.0 60-61 35.31061093822065 38.0 35.0 40.0 28.0 41.0 62-63 34.89888736548236 37.5 34.0 40.0 28.0 41.0 64-65 34.475018577545626 37.0 34.0 39.5 27.0 41.0 66-67 34.099840494483644 36.0 34.0 39.0 26.0 41.0 68-69 33.6190946399397 36.0 33.0 39.0 26.0 40.0 70-71 33.15507999097426 35.0 33.0 38.5 25.5 40.0 72-73 32.673002773232184 35.0 32.0 37.5 24.5 39.5 74-75 32.170632523244265 35.0 32.0 37.0 22.5 39.0 76-77 30.208532370587463 33.5 29.5 35.0 20.0 37.5 78-79 31.181343148276937 34.0 31.0 36.0 20.0 38.0 80-81 31.213772297190438 35.0 31.0 35.5 20.0 37.0 82-83 30.92099050907438 34.5 31.0 35.0 19.5 37.0 84-85 30.53882640863454 34.0 31.0 35.0 18.0 36.5 86-87 29.982540746529487 34.0 30.5 35.0 10.5 36.0 88-89 29.431474001692656 34.0 30.0 35.0 6.0 36.0 90-91 28.97303427069155 34.0 29.0 35.0 2.0 35.0 92-93 28.672854549894026 34.0 29.0 35.0 2.0 35.0 94-95 28.319613686307804 34.0 29.0 35.0 2.0 35.0 96-97 27.737164123599737 34.0 28.5 35.0 2.0 35.0 98-99 26.920109936720543 33.0 26.5 35.0 2.0 35.0 100-101 23.19864673415681 29.0 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16872283579809277 1101 2 0.1530344464349298 1101 3 0.147421522593568 1101 4 0.13725345118113097 1101 5 0.14350994713213794 1101 6 0.17288006488119123 1101 7 0.18762622619408376 1101 8 0.22507565807624275 1101 9 0.24345439296634908 1101 10-11 0.22223559265929538 1101 12-13 0.14901202851466877 1101 14-15 0.2880195617249868 1101 16-17 0.21304723867641684 1101 18-19 0.2968109546708817 1101 20-21 0.276217218773148 1101 22-23 0.2216081355920565 1101 24-25 0.2408655485236011 1101 26-27 0.24670981360178956 1101 28-29 0.2519209521838306 1101 30-31 0.294302314814999 1101 32-33 0.2807919612617411 1101 34-35 0.3701376183867282 1101 36-37 0.383246956059331 1101 38-39 0.3306726449109121 1101 40-41 0.4002733047341209 1101 42-43 0.4624696752347788 1101 44-45 0.4749031245429265 1101 46-47 0.5250087514498603 1101 48-49 0.42266656700296323 1101 50-51 0.3811561314451595 1101 52-53 0.35450146187586284 1101 54-55 0.3039544413421851 1101 56-57 0.28275424141528305 1101 58-59 0.43421314868415806 1101 60-61 0.4343181194403414 1101 62-63 0.24023745408877772 1101 64-65 0.30700175280546915 1101 66-67 0.3166693076877962 1101 68-69 0.23754232533242003 1101 70-71 0.2515540821306459 1101 72-73 0.19524030123123737 1101 74-75 0.3339861418992811 1101 76-77 0.26425403792668334 1101 78-79 0.33700843726108687 1101 80-81 0.4384306157988682 1101 82-83 0.49402960099927284 1101 84-85 0.5285031608227726 1101 86-87 0.5327957812028465 1101 88-89 0.6965629323106164 1101 90-91 0.6226124370070529 1101 92-93 0.39800940025509135 1101 94-95 0.22550815408522595 1101 96-97 0.14438677268221056 1101 98-99 0.08003353906157429 1101 100-101 -0.3093799432267623 1102 1 0.15511532610951662 1102 2 0.10889158499778517 1102 3 0.1710871464085102 1102 4 0.1572134540021466 1102 5 0.08703308063565629 1102 6 0.12863184882821344 1102 7 0.11504368829533007 1102 8 0.1414018371230128 1102 9 0.26633440952248577 1102 10-11 0.21251998942391737 1102 12-13 0.19309346922241843 1102 14-15 0.2345638002582504 1102 16-17 0.2181231200133027 1102 18-19 0.19956495901339366 1102 20-21 0.2517219092936003 1102 22-23 0.23872305796781035 1102 24-25 0.2236584950537548 1102 26-27 0.22806898352881433 1102 28-29 0.19762285960819526 1102 30-31 0.2714198414296831 1102 32-33 0.2760721756359814 1102 34-35 0.29543021228963084 1102 36-37 0.3779810317597381 1102 38-39 0.4544891758449907 1102 40-41 0.4566925080046431 1102 42-43 0.4446853391683021 1102 44-45 0.42511779360514623 1102 46-47 0.5041687702258173 1102 48-49 0.3613342834582838 1102 50-51 0.38278284139826013 1102 52-53 0.3911862570514515 1102 54-55 0.33574718381099444 1102 56-57 0.3333467129969918 1102 58-59 0.24254362053410006 1102 60-61 0.35887308651305716 1102 62-63 0.37409157739963916 1102 64-65 0.2996545328955591 1102 66-67 0.3477550614314282 1102 68-69 0.3085185305083016 1102 70-71 0.18934355441529505 1102 72-73 0.11806486570768726 1102 74-75 0.2053002477984478 1102 76-77 0.36001603650229796 1102 78-79 0.4268529459877435 1102 80-81 0.3414239442069835 1102 82-83 0.33598084074417756 1102 84-85 0.4232093876418652 1102 86-87 0.41027399924441 1102 88-89 0.2699288374180462 1102 90-91 0.41001368692612417 1102 92-93 0.4617993078320701 1102 94-95 0.2039805497306837 1102 96-97 0.17694727426844992 1102 98-99 0.19926577127297307 1102 100-101 -0.3270725094975653 1103 1 0.03286818855842455 1103 2 0.06724657854800853 1103 3 0.01157734890357176 1103 4 0.023535419247693312 1103 5 0.06919145402736149 1103 6 0.08790480480200813 1103 7 0.10898327425064025 1103 8 0.16402128722656073 1103 9 0.12783394546927696 1103 10-11 0.13554267043280532 1103 12-13 0.07131351145758202 1103 14-15 0.1287304867265604 1103 16-17 0.124086374002502 1103 18-19 0.14383980413329311 1103 20-21 0.17077189461259934 1103 22-23 0.17860464831895229 1103 24-25 0.14252366504980074 1103 26-27 0.22087994538418343 1103 28-29 0.22331573294869145 1103 30-31 0.18503959126755376 1103 32-33 0.18666512294430504 1103 34-35 0.13676500691465066 1103 36-37 0.2524846184971068 1103 38-39 0.19617536970960003 1103 40-41 0.1687749000652019 1103 42-43 0.21803762371497726 1103 44-45 0.2658716011134601 1103 46-47 0.12244432375813119 1103 48-49 0.05259611771840156 1103 50-51 0.07026207572922516 1103 52-53 0.13406200848901761 1103 54-55 0.19495236953441974 1103 56-57 0.10043320226039754 1103 58-59 0.15896672700731784 1103 60-61 0.14194283567697852 1103 62-63 0.1774277163129483 1103 64-65 0.1502287735581831 1103 66-67 0.19641333692659657 1103 68-69 0.22468178385827997 1103 70-71 0.16311193746047792 1103 72-73 0.1179163881343257 1103 74-75 0.09916657462725453 1103 76-77 -0.02498797871481173 1103 78-79 0.0853692569127027 1103 80-81 0.11590638140138765 1103 82-83 0.14621746966821902 1103 84-85 -0.03229020916891656 1103 86-87 0.2602405032041126 1103 88-89 0.287450511183426 1103 90-91 0.4026979877537755 1103 92-93 0.31540570094148634 1103 94-95 0.14901716748243032 1103 96-97 0.35214150058217797 1103 98-99 0.3685195050635919 1103 100-101 9.251498789026869E-4 1104 1 0.005867794286579908 1104 2 0.07450607874621795 1104 3 0.064719732966104 1104 4 0.025474390891439214 1104 5 0.1518270047137804 1104 6 0.09186050502799503 1104 7 0.10130220028947434 1104 8 0.04080705361152326 1104 9 0.037693444971552026 1104 10-11 0.1558708903663657 1104 12-13 0.1156405204706985 1104 14-15 0.13583761812186168 1104 16-17 0.09198473644941174 1104 18-19 0.1583635555990952 1104 20-21 0.09786606562593647 1104 22-23 0.1333784826942619 1104 24-25 0.11586554852360109 1104 26-27 0.17524358179648658 1104 28-29 0.1252287008520696 1104 30-31 0.23779325755404557 1104 32-33 0.13356623458747663 1104 34-35 0.160498606022351 1104 36-37 0.33259146268692774 1104 38-39 0.2688638540006849 1104 40-41 0.30236714214436944 1104 42-43 0.25832595607890596 1104 44-45 0.254659077348073 1104 46-47 0.23548317198114432 1104 48-49 0.2313116078909232 1104 50-51 0.35242989367145583 1104 52-53 0.14233395488303557 1104 54-55 0.09688220038830764 1104 56-57 0.17346637329006143 1104 58-59 0.14026444346938405 1104 60-61 0.2699186445699624 1104 62-63 0.2482151705989537 1104 64-65 0.1273589322492512 1104 66-67 0.18779750679164664 1104 68-69 0.1510593463910581 1104 70-71 0.15675989151616676 1104 72-73 0.0996111200147567 1104 74-75 0.07050645114227905 1104 76-77 0.09125523160552973 1104 78-79 0.16554348598812396 1104 80-81 0.09361262398536851 1104 82-83 0.1881409059842163 1104 84-85 0.18388880148980746 1104 86-87 0.37280384298908587 1104 88-89 0.3979061829030037 1104 90-91 0.4211455872057037 1104 92-93 0.45675229051681043 1104 94-95 0.3523810718282796 1104 96-97 0.20540191191504675 1104 98-99 0.17476453818858317 1104 100-101 -0.2730733715347675 1105 1 0.023767181696101147 1105 2 0.09321945197817172 1105 3 0.06681072713902125 1105 4 0.06259297446272427 1105 5 0.10664741941594968 1105 6 0.08767877607853336 1105 7 0.09502818018521708 1105 8 0.0693886431094981 1105 9 0.07251481425237927 1105 10-11 0.13153004249300437 1105 12-13 0.11264319536610401 1105 14-15 0.1369360320686681 1105 16-17 0.16505059926399923 1105 18-19 0.1660598460234297 1105 20-21 0.18392664920551738 1105 22-23 0.20428551918407578 1105 24-25 0.09154026443269458 1105 26-27 0.1365856104421468 1105 28-29 0.14610141920156394 1105 30-31 0.16370277906111141 1105 32-33 0.20753894712648702 1105 34-35 0.2089116968235416 1105 36-37 0.15632501176887104 1105 38-39 0.20879504651845338 1105 40-41 0.1771190295123901 1105 42-43 0.2665075771194836 1105 44-45 0.17812339893317386 1105 46-47 0.1825189717935416 1105 48-49 0.20397719616038756 1105 50-51 0.22919780966023495 1105 52-53 0.1050384629845027 1105 54-55 0.22194484461269326 1105 56-57 0.18340903631550276 1105 58-59 0.22205873488149308 1105 60-61 0.1856259769791464 1105 62-63 0.30031661844576263 1105 64-65 0.06246703828218614 1105 66-67 0.09898031794278239 1105 68-69 0.10337866125259865 1105 70-71 0.2088191874521499 1105 72-73 -0.029334428535939594 1105 74-75 -0.050415708156151595 1105 76-77 -0.021976214290614138 1105 78-79 0.1283964985138546 1105 80-81 0.08155873386982293 1105 82-83 0.08470050593275147 1105 84-85 0.1508354778959422 1105 86-87 0.3001617041518472 1105 88-89 0.48014715487159165 1105 90-91 0.35189025712900346 1105 92-93 0.3158438999779314 1105 94-95 0.2682170648391029 1105 96-97 0.15334689601923657 1105 98-99 0.034886470048270724 1105 100-101 -0.054239456807696484 1106 1 0.043578889347244854 1106 2 0.08724050297517749 1106 3 0.07110319743589599 1106 4 0.06755212270761035 1106 5 0.025705400240312315 1106 6 0.05951729418708851 1106 7 0.08512406833263242 1106 8 0.03554384991653592 1106 9 0.07766707264570272 1106 10-11 0.10725228937934617 1106 12-13 0.10514201082447272 1106 14-15 0.06855824192565052 1106 16-17 0.019582541076132998 1106 18-19 5.42052018204231E-4 1106 20-21 0.13092450807186395 1106 22-23 0.1278900495083377 1106 24-25 0.09782069918932734 1106 26-27 0.16004737363734023 1106 28-29 0.08883443048556927 1106 30-31 0.17908079217667705 1106 32-33 0.13351965303366597 1106 34-35 0.023719935459901365 1106 36-37 0.07371281377721317 1106 38-39 0.1859220701933566 1106 40-41 -0.0035779438344363257 1106 42-43 0.014801687857200818 1106 44-45 0.009273210601008941 1106 46-47 0.06219927993363683 1106 48-49 0.11889201865832177 1106 50-51 0.10599888530366997 1106 52-53 0.10177101936471189 1106 54-55 0.01899988996372315 1106 56-57 0.15235264417049876 1106 58-59 0.1990914431617412 1106 60-61 0.1265539309671766 1106 62-63 0.15432198670880837 1106 64-65 0.18895791830703246 1106 66-67 0.08939909322628381 1106 68-69 0.005972011096517349 1106 70-71 0.017488339997221658 1106 72-73 0.08714172435154666 1106 74-75 0.1183432754474083 1106 76-77 0.19595879120406678 1106 78-79 0.2584841794799644 1106 80-81 0.1437021271137553 1106 82-83 0.16588951199282675 1106 84-85 0.1821949733409305 1106 86-87 0.16023672843992998 1106 88-89 0.2963056714273016 1106 90-91 0.4572098195852057 1106 92-93 0.40235463351777767 1106 94-95 0.15790513222459523 1106 96-97 0.17986383098292436 1106 98-99 0.19010957697156528 1106 100-101 -0.11073076983725727 1107 1 -0.0032866087377456665 1107 2 0.05180182396958344 1107 3 0.04342700320064807 1107 4 0.03919595621958649 1107 5 0.0793263056646083 1107 6 0.06729573242530051 1107 7 0.044742532285475534 1107 8 0.09618936267871447 1107 9 0.16591125760666614 1107 10-11 0.12493540860138097 1107 12-13 0.10107535369049714 1107 14-15 0.09441910877800552 1107 16-17 0.1462504761419794 1107 18-19 0.1304533861889965 1107 20-21 0.09292240221409998 1107 22-23 0.10194356571174268 1107 24-25 0.10414548106997756 1107 26-27 0.12587309559084758 1107 28-29 0.09625178856765615 1107 30-31 0.198108020610249 1107 32-33 0.1701651232532697 1107 34-35 0.2270926167415226 1107 36-37 0.2841495724579701 1107 38-39 0.2931951380215949 1107 40-41 0.2104849199194092 1107 42-43 0.2758053264296194 1107 44-45 0.17746335466673457 1107 46-47 0.29441683560237664 1107 48-49 0.18498250909156155 1107 50-51 0.14617793048270755 1107 52-53 0.1893202830826155 1107 54-55 0.06745358531865975 1107 56-57 0.15633344801256754 1107 58-59 0.1932999798033066 1107 60-61 0.24778403787903613 1107 62-63 0.09613064908733548 1107 64-65 0.19325820511492964 1107 66-67 0.2279376492129117 1107 68-69 0.07330598571986968 1107 70-71 0.16144475275952885 1107 72-73 0.1002743236677972 1107 74-75 0.11335976660623004 1107 76-77 0.11164843598533025 1107 78-79 0.35156829207126705 1107 80-81 0.251881174656269 1107 82-83 0.22401709482972976 1107 84-85 0.26660276288751206 1107 86-87 0.27424942740035263 1107 88-89 0.3920193882189764 1107 90-91 0.45627671897554833 1107 92-93 0.4403876418336701 1107 94-95 0.24177517173010799 1107 96-97 0.23396924913513573 1107 98-99 0.32089876548365126 1107 100-101 -0.011856434712800734 1108 1 0.03700088559654091 1108 2 0.06940395455822568 1108 3 0.06047452760191874 1108 4 0.03537621167626526 1108 5 0.03577029466256931 1108 6 0.03926700551395612 1108 7 0.03566894983918445 1108 8 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92-93 -0.2176949722590038 2316 94-95 -0.057873518518409384 2316 96-97 -0.20244404473809752 2316 98-99 -0.062036265764568554 2316 100-101 0.2642431073867222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 13.0 5 60.0 6 109.0 7 281.0 8 658.0 9 1215.0 10 2187.0 11 3388.0 12 5150.0 13 7169.0 14 9291.0 15 12347.0 16 15698.0 17 22307.0 18 22253.0 19 16423.0 20 15282.0 21 15980.0 22 16857.0 23 18611.0 24 21388.0 25 24885.0 26 29084.0 27 34908.0 28 42613.0 29 51936.0 30 63277.0 31 77738.0 32 97313.0 33 126901.0 34 176904.0 35 260353.0 36 428208.0 37 722340.0 38 621212.0 39 58118.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70440834157551 19.016988667470798 14.965791253437558 23.31281173751614 2 15.759912601167391 20.22206370559669 39.80580757566835 24.212216117567575 3 19.87628644954153 23.67437714766684 29.22130472269994 27.228031680091686 4 13.637969790230892 16.661990573260375 35.936252053786546 33.76378758272219 5 14.484033898148068 36.652719997511966 33.6246627753629 15.238583328977068 6 32.896714140043265 35.3180482427263 16.9889752275129 14.796262389717535 7 29.332207541788723 31.870002838741613 20.20515698108763 18.592632638382035 8 26.195861260308632 35.30868502739154 19.25232574990091 19.243127962398916 9 26.003270177398193 17.44307462846563 20.20214619453122 36.35150899960496 10-11 25.461675995693582 26.238243871064228 26.61297099785407 21.687109135388123 12-13 26.358179049822233 23.395829762849278 27.312863072735972 22.93312811459252 14-15 22.90156158703669 25.05042287054358 25.605785792793768 26.442229749625962 16-17 22.679562535257404 28.16219810978969 26.370634079039444 22.787605275913467 18-19 22.61467047591949 26.798540264320682 28.428501239882014 22.158288019877816 20-21 23.248532820551166 25.91063059104718 28.558021827209966 22.282814761191688 22-23 22.989323949384378 25.767998713633173 28.490990469371695 22.75168686761075 24-25 22.955312816898847 25.98912142612545 28.448711395206878 22.60685436176883 26-27 22.596352441286943 26.156233431184983 28.57029892313559 22.67711520439249 28-29 22.50505051173446 26.549893365218995 28.509898870657267 22.435157252389274 30-31 22.70787118918743 26.078834477771668 28.29120387457461 22.922090458466293 32-33 22.5384173056041 26.14272584042302 28.378201772989343 22.94065508098354 34-35 23.10415272795135 26.210376823448673 28.127384471625906 22.55808597697407 36-37 23.115596681867576 25.898704991114922 28.25740794029202 22.728290386725487 38-39 22.684555052221338 26.232250519466728 28.29060949107649 22.792584937235446 40-41 22.99235805638616 26.14114192864067 28.18775134870237 22.678748666270803 42-43 22.78120219186767 26.19705731458324 27.889863298624846 23.131877194924247 44-45 22.89705425222925 26.25719488342523 27.94421338064273 22.901537483702793 46-47 23.172406582648204 26.584229714425245 27.454257847408265 22.78910585551829 48-49 23.122366373218 26.551606512337017 27.4289913341768 22.89703578026818 50-51 22.9068288786092 26.801430818647887 27.435929376199663 22.855810926543246 52-53 23.269879565109648 26.710235989654795 27.140431495770173 22.87945294946539 54-55 22.934087596022586 26.59171352134317 27.295129209063063 23.179069673571185 56-57 22.983583603587267 26.83881307515122 27.23031458418391 22.947288737077603 58-59 23.19158503260234 26.48571680978477 27.23599912517797 23.08669903243492 60-61 23.01406061315967 26.588798661092518 27.545943463387427 22.851197262360383 62-63 22.85774220412879 26.69786658469919 27.51016845573475 22.93422275543727 64-65 22.63207454465867 26.713346962665135 27.398695673608053 23.255882819068137 66-67 22.65887821632157 26.69978892172402 27.247179625975054 23.39415323597936 68-69 22.7154592110039 26.499058513135655 27.50225238862381 23.283229887236644 70-71 22.85766371857677 26.55598550256405 27.254918211638806 23.33143256722037 72-73 22.88427727557564 26.483606432628193 27.349505337694453 23.282610954101717 74-75 22.69421723850657 26.989368945754887 26.967168530597203 23.349245285141336 76-77 22.794457749591423 26.644394038513123 27.137205777664747 23.423942434230703 78-79 22.833742327025778 26.708980998584263 27.18137781190746 23.275898862482503 80-81 22.86875201931996 26.811374464347722 27.041905738433776 23.277967777898546 82-83 22.850521463684807 26.7481324777813 27.170570668734744 23.23077538979915 84-85 22.840405814325123 26.757588009261312 27.01990692368417 23.382099252729393 86-87 22.867517458759174 26.753216470728127 27.00296917729329 23.376296893219415 88-89 22.795497933629665 26.763440542315497 26.829728493454752 23.611333030600086 90-91 22.719504164760256 26.722440380944157 26.96554180911107 23.592513645184514 92-93 22.803598121533874 26.610009839647493 27.022553769008482 23.563838269810148 94-95 22.665234282205322 26.462828696832318 27.166145127852552 23.705791893109797 96-97 22.642769182515206 26.300775591851156 27.30186212185679 23.75459310377685 98-99 22.573304877187333 26.51422523311966 26.873505857954953 24.038964031738054 100-101 22.723052024697267 25.742550016303056 27.504322253596804 24.03007570540287 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 503.0 1 377.0 2 315.5 3 414.0 4 376.5 5 347.0 6 587.0 7 1316.0 8 2212.5 9 2664.5 10 2488.5 11 2183.0 12 2280.5 13 2525.0 14 2771.0 15 2924.0 16 2990.0 17 3216.0 18 3507.0 19 3897.5 20 4395.5 21 5125.5 22 6242.0 23 7799.0 24 9647.0 25 12054.5 26 15314.0 27 19152.0 28 22762.5 29 26674.5 30 31111.5 31 36136.0 32 42797.0 33 47314.0 34 50274.0 35 53977.5 36 58952.0 37 64981.0 38 69853.0 39 73474.5 40 78007.0 41 84637.5 42 90201.5 43 95303.5 44 101398.0 45 106690.0 46 109800.5 47 116125.0 48 126656.5 49 126846.5 50 119408.0 51 115289.5 52 112981.0 53 113422.0 54 112765.5 55 105412.5 56 96104.5 57 87899.0 58 80947.0 59 72089.0 60 59769.5 61 48845.5 62 39865.0 63 30875.5 64 23077.0 65 17460.0 66 13009.5 67 10429.0 68 8251.5 69 6069.5 70 4426.0 71 3178.5 72 2493.5 73 2092.5 74 1608.5 75 1229.0 76 853.5 77 483.0 78 293.0 79 187.5 80 121.5 81 76.0 82 44.0 83 25.0 84 13.5 85 8.0 86 4.5 87 4.0 88 3.5 89 2.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3479609034477145 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.263958635101272E-4 16-17 0.0016873638942505888 18-19 3.3085566553933114E-5 20-21 0.0 22-23 0.0 24-25 3.6394123209326426E-4 26-27 0.0011083664795567593 28-29 0.0 30-31 0.0031596716059006124 32-33 0.0 34-35 0.010190354498611399 36-37 0.027345220756825717 38-39 0.02041379456377673 40-41 0.013052256005526612 42-43 0.009743699350133302 44-45 0.003060414906238813 46-47 0.001157994829387659 48-49 5.955401979707961E-4 50-51 1.6542783276966557E-5 52-53 9.925669966179932E-5 54-55 0.0 56-57 0.0 58-59 0.004532722617888837 60-61 0.0099422127494569 62-63 0.004268038085457371 64-65 0.0020843906928977862 66-67 0.004218409735626472 68-69 0.0039371824199180405 70-71 1.6542783276966557E-5 72-73 0.0 74-75 0.0 76-77 0.21528778156644277 78-79 2.315989658775318E-4 80-81 0.0032920138721163447 82-83 3.970267986471973E-4 84-85 0.0 86-87 0.10850411551362364 88-89 0.40083163880089967 90-91 0.3900292013210405 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012407087457724916 100-101 8.767675136792274E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3022466.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.44849540674763 #Duplication Level Percentage of deduplicated Percentage of total 1 75.09064843319979 40.8857282630478 2 13.952082872810182 15.19339840429535 3 4.616590777705689 7.540992652642251 4 2.063720010651275 4.494657980830365 5 1.1404596865267018 3.1048157001714976 6 0.6879871631939382 2.2475919537039886 7 0.46742135576341093 1.781527267960988 8 0.329057185127069 1.4333334906358817 9 0.22910277530330583 1.1226871267897665 >10 1.2978281544711086 12.821397214417091 >50 0.08608708267505043 3.2091823127274406 >100 0.034755451500301904 3.3558616241292567 >500 0.0029204921639389334 1.1037394869735349 >1k 0.001156028147890269 1.0080314884983324 >5k 1.8253076019320036E-4 0.6970550331764628 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8662 0.2865871774901686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7321 0.2422194327413443 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5058 0.16734679562979368 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4597 0.1520943494484305 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.3085566553933114E-5 0.0 0.0 0.0 0.0 9 3.3085566553933114E-5 0.0 0.0 0.0 0.0 10-11 3.3085566553933114E-5 0.0 0.0 0.0 0.0 12-13 6.617113310786623E-5 0.0 0.0 3.3085566553933114E-5 0.0 14-15 6.617113310786623E-5 0.0 0.0 3.3085566553933114E-5 0.0 16-17 6.617113310786623E-5 0.0 0.0 3.3085566553933114E-5 0.0 18-19 9.925669966179935E-5 0.0 0.0 6.617113310786623E-5 0.0 20-21 1.3234226621573246E-4 0.0 0.0 6.617113310786623E-5 0.0 22-23 1.3234226621573246E-4 0.0 0.0 9.925669966179934E-5 0.0 24-25 1.3234226621573246E-4 0.0 0.0 1.4888504949269902E-4 0.0 26-27 1.3234226621573246E-4 0.0 0.0 4.301123652011305E-4 0.0 28-29 2.481417491544983E-4 0.0 0.0 0.0014723077116500236 0.0 30-31 2.646845324314649E-4 0.0 0.0 0.005062091682751766 1.6542783276966557E-5 32-33 2.646845324314649E-4 0.0 0.0 0.013234226621573246 3.3085566553933114E-5 34-35 2.646845324314649E-4 0.0 0.0 0.026385739326761658 3.3085566553933114E-5 36-37 2.646845324314649E-4 0.0 0.0 0.047345445738678285 3.3085566553933114E-5 38-39 2.97770098985398E-4 0.0 0.0 0.07801576593417428 3.3085566553933114E-5 40-41 3.143128822623646E-4 0.0 0.0 0.11207735670144842 3.3085566553933114E-5 42-43 3.639412320932642E-4 0.0 0.0 0.1491497340251305 3.3085566553933114E-5 44-45 4.6319793175506356E-4 0.0 0.0 0.19485744421938905 4.9628349830899675E-5 46-47 5.459118481398964E-4 0.0 0.0 0.2513179635436759 6.617113310786623E-5 48-49 5.95540197970796E-4 0.0 0.0 0.31391585546371736 6.617113310786623E-5 50-51 5.95540197970796E-4 0.0 0.0 0.38258494884640554 6.617113310786623E-5 52-53 5.95540197970796E-4 0.0 0.0 0.44981482008399765 6.617113310786623E-5 54-55 6.286257645247292E-4 0.0 0.0 0.5280621849840494 6.617113310786623E-5 56-57 6.617113310786623E-4 0.0 0.0 0.6235140444921465 6.617113310786623E-5 58-59 6.617113310786623E-4 0.0 0.0 0.7280313492360211 6.617113310786623E-5 60-61 6.617113310786623E-4 0.0 0.0 0.8439466316577258 6.617113310786623E-5 62-63 6.617113310786623E-4 0.0 0.0 0.9643946366973193 9.925669966179934E-5 64-65 6.617113310786623E-4 0.0 0.0 1.0931636617252272 9.925669966179934E-5 66-67 6.947968976325953E-4 0.0 0.0 1.2499065332744852 9.925669966179934E-5 68-69 6.947968976325953E-4 0.0 0.0 1.4259548329079632 9.925669966179934E-5 70-71 7.278824641865284E-4 0.0 0.0 1.6181819745863146 9.925669966179934E-5 72-73 7.775108140174281E-4 0.0 0.0 1.8241230836012712 1.3234226621573246E-4 74-75 7.940535972943947E-4 0.0 0.0 2.034977399249487 1.3234226621573246E-4 76-77 7.940535972943947E-4 0.0 0.0 2.2685945846868085 1.3234226621573246E-4 78-79 7.940535972943947E-4 0.0 0.0 2.541385080923987 1.3234226621573246E-4 80-81 8.93310296956194E-4 0.0 0.0 2.8394364072250937 1.3234226621573246E-4 82-83 8.93310296956194E-4 0.0 0.0 3.1476450024582574 1.3234226621573246E-4 84-85 9.263958635101271E-4 0.0 0.0 3.468442655765193 1.3234226621573246E-4 86-87 9.594814300640603E-4 0.0 0.0 3.8075862557262843 1.3234226621573246E-4 88-89 9.594814300640603E-4 0.0 0.0 4.186829562350743 1.3234226621573246E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15150 0.0 25.047274 1 GGTATCA 11200 0.0 24.61361 1 GTACATG 10515 0.0 23.77195 1 TACATGG 10555 0.0 23.062084 2 ACATGGG 10970 0.0 21.411053 3 TCAACGC 17555 0.0 21.245216 4 CAACGCA 17760 0.0 21.106857 5 ATCAACG 17680 0.0 21.09501 3 TATCAAC 17825 0.0 21.003271 2 GAGTACT 9520 0.0 20.71003 12-13 AACGCAG 18260 0.0 20.476933 6 GTACTTT 9830 0.0 20.032782 14-15 AGAGTAC 15165 0.0 19.274582 10-11 TAAGGTG 1450 0.0 18.980143 5 AGTACTT 9885 0.0 18.673235 12-13 CATGGGG 6775 0.0 18.48988 4 ACGCAGA 20265 0.0 18.404135 7 CGCAGAG 20575 0.0 18.126844 8 GCAGAGT 22165 0.0 16.634125 9 GTGTAGC 1755 0.0 16.548836 1 >>END_MODULE