FastQCFastQC Report
Fri 27 May 2016
SRR939098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975621
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80370.27009488103491675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64050.21524918664036852No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44760.15042238242034184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39680.13335031578282316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141800.026.4895481
GGTATCA104400.024.8806151
GTACATG109450.022.3008611
ATCAACG165950.022.2045783
TCAACGC166700.022.133094
TATCAAC169050.022.0495512
CAACGCA169400.021.807915
TACATGG109850.021.6011982
AACGCAG171950.021.5298146
ACATGGG109550.021.0983093
GAGTACT85400.020.53923412-13
GGACCGA5500.019.7970646
AGAGTAC142300.019.7781810-11
GTACTTT90200.019.28926314-15
ACGCAGA191350.019.1985727
CGCAGAG193200.018.9657278
AGGACCG8100.018.7110375
GTGTAGC17800.018.6746441
AGTACTT91750.018.34372112-13
CATGGGG68200.018.1257654
Produced by FastQC (version 0.11.5)