##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939098_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2975621 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03291682643724 33.0 31.0 34.0 30.0 34.0 2 32.14660603618539 34.0 31.0 34.0 30.0 34.0 3 32.21138075043831 34.0 31.0 34.0 30.0 34.0 4 35.646672072821104 37.0 35.0 37.0 35.0 37.0 5 35.67510949815181 37.0 35.0 37.0 35.0 37.0 6 35.75335635821901 37.0 36.0 37.0 35.0 37.0 7 35.67828160911621 37.0 36.0 37.0 35.0 37.0 8 35.66728088019274 37.0 36.0 37.0 35.0 37.0 9 37.337357479329526 39.0 38.0 39.0 35.0 39.0 10-11 37.23644459425444 39.0 37.0 39.0 34.0 39.0 12-13 37.12999622599787 39.0 37.0 39.0 34.0 39.0 14-15 38.40479449499785 40.0 38.0 41.0 34.0 41.0 16-17 38.30034167657776 40.0 38.0 41.0 33.5 41.0 18-19 38.17014246774035 40.0 38.0 41.0 33.0 41.0 20-21 38.143875681748455 40.0 38.0 41.0 33.5 41.0 22-23 38.19395648841032 40.0 38.0 41.0 34.0 41.0 24-25 38.13314985342556 40.0 38.0 41.0 33.0 41.0 26-27 38.00864071734942 40.0 38.0 41.0 33.0 41.0 28-29 37.85566693473396 40.0 38.0 41.0 33.0 41.0 30-31 37.64157011259162 40.0 38.0 41.0 32.5 41.0 32-33 37.43432396128405 40.0 37.0 41.0 31.5 41.0 34-35 37.11173348353167 40.0 37.0 41.0 31.0 41.0 36-37 36.83886422363601 40.0 37.0 41.0 30.0 41.0 38-39 36.641847197610176 40.0 36.0 41.0 30.0 41.0 40-41 36.443803663168126 40.0 36.0 41.0 30.0 41.0 42-43 36.22727239120842 39.5 36.0 41.0 29.5 41.0 44-45 35.95404253431469 39.0 35.0 41.0 28.5 41.0 46-47 35.82201446353551 39.0 35.0 41.0 27.5 41.0 48-49 35.69071262771703 39.0 35.0 41.0 27.0 41.0 50-51 34.57717380674488 38.0 34.0 40.0 25.0 40.5 52-53 34.726225550901816 38.5 34.5 39.5 25.5 40.5 54-55 35.23848215212892 39.0 35.0 40.5 26.0 41.0 56-57 35.12792573382161 39.0 35.0 41.0 26.0 41.0 58-59 34.90352349980055 39.0 35.0 41.0 26.0 41.0 60-61 34.582977637273025 38.0 34.5 40.0 24.5 41.0 62-63 34.18522705008468 37.5 34.0 40.0 23.0 41.0 64-65 33.79726164723263 37.0 34.0 40.0 22.0 41.0 66-67 33.39072801946216 36.5 33.5 39.5 21.5 41.0 68-69 32.96772841702622 36.0 33.0 39.0 20.0 41.0 70-71 32.52861738776544 35.5 33.0 39.0 18.5 40.5 72-73 32.07791365230989 35.0 32.0 38.0 17.5 40.0 74-75 31.625637976072895 35.0 32.0 37.0 13.0 39.5 76-77 31.181050778980254 35.0 31.0 37.0 9.0 39.0 78-79 30.75091233056898 35.0 31.0 36.0 7.0 39.0 80-81 30.29468084141092 35.0 31.0 36.0 2.0 37.5 82-83 29.87909716996889 34.0 30.0 35.0 2.0 37.0 84-85 29.49229102093311 34.0 30.0 35.0 2.0 36.5 86-87 29.070641220773748 34.0 29.0 35.0 2.0 36.0 88-89 28.630587363108408 34.0 29.0 35.0 2.0 36.0 90-91 28.202715836459014 34.0 29.0 35.0 2.0 35.5 92-93 27.76671121759122 34.0 27.5 35.0 2.0 35.0 94-95 27.262981407914516 34.0 26.5 35.0 2.0 35.0 96-97 26.62305347354384 33.0 25.0 35.0 2.0 35.0 98-99 25.84652581763605 33.0 23.5 35.0 2.0 35.0 100-101 22.807240068543678 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23633523736491213 1101 2 0.34042713167265504 1101 3 0.27323338635795835 1101 4 0.3999963123035428 1101 5 0.2988632605060104 1101 6 0.22446546270056444 1101 7 0.22769954543269932 1101 8 0.3275810895655056 1101 9 0.45786676055434583 1101 10-11 0.4481028060957257 1101 12-13 0.4568716943694824 1101 14-15 0.5507621696175917 1101 16-17 0.5194014408218024 1101 18-19 0.6355810744924355 1101 20-21 0.6658411416293788 1101 22-23 0.746386233620747 1101 24-25 0.6493510799795885 1101 26-27 0.6533306257452267 1101 28-29 0.6669577822413473 1101 30-31 0.6929813922226202 1101 32-33 0.7093408758306126 1101 34-35 0.7104340196262129 1101 36-37 0.7406333859409244 1101 38-39 0.615139513966021 1101 40-41 0.5551255201536733 1101 42-43 0.6280966018258312 1101 44-45 0.5768068890700491 1101 46-47 0.6711361427875246 1101 48-49 0.7597935040511814 1101 50-51 0.7744045532482033 1101 52-53 0.7898908086093286 1101 54-55 0.8769185584317398 1101 56-57 0.8041913477350633 1101 58-59 0.7483297417412516 1101 60-61 0.686179879441859 1101 62-63 0.5674574802486134 1101 64-65 0.5272765701848172 1101 66-67 0.556525168114888 1101 68-69 0.6081427886629953 1101 70-71 0.5433829362296052 1101 72-73 0.5014653450394491 1101 74-75 0.5327623670742767 1101 76-77 0.6300032420978603 1101 78-79 0.5506941195650796 1101 80-81 0.39323335876690635 1101 82-83 0.40323973959882764 1101 84-85 0.27876260993265234 1101 86-87 0.15087100964024458 1101 88-89 0.14252164661742128 1101 90-91 0.2932556554176635 1101 92-93 0.16559525432711553 1101 94-95 -0.06485024720362986 1101 96-97 -0.1337660894173247 1101 98-99 -0.5078788488645074 1101 100-101 -0.380415146062667 1102 1 0.23173022080595018 1102 2 0.2758213796870024 1102 3 0.25164920609775976 1102 4 0.19602660951082385 1102 5 0.2904156394927355 1102 6 0.28555895915628327 1102 7 0.23946488975473557 1102 8 0.3274950920896842 1102 9 0.4016398316013081 1102 10-11 0.366205482337179 1102 12-13 0.4421751705653776 1102 14-15 0.4458384286206396 1102 16-17 0.3603958148076245 1102 18-19 0.3427059950189104 1102 20-21 0.44346427222217955 1102 22-23 0.5605891319136092 1102 24-25 0.6282790295150846 1102 26-27 0.5166655129639821 1102 28-29 0.5337192240572293 1102 30-31 0.6077236429604156 1102 32-33 0.6211334131475894 1102 34-35 0.48489437063825136 1102 36-37 0.5362031518380022 1102 38-39 0.4707415971214317 1102 40-41 0.5825452222346925 1102 42-43 0.5125036877806082 1102 44-45 0.44096349699747606 1102 46-47 0.5969344069432978 1102 48-49 0.6891700045588607 1102 50-51 0.6373186459214537 1102 52-53 0.6981098542627109 1102 54-55 0.6721679095956148 1102 56-57 0.7966874748832211 1102 58-59 0.7288428619906284 1102 60-61 0.5419111070825124 1102 62-63 0.5018391821208681 1102 64-65 0.5269134532567179 1102 66-67 0.6054577318597794 1102 68-69 0.5520599796177237 1102 70-71 0.4052530492006312 1102 72-73 0.2686110176464567 1102 74-75 0.24462856567743785 1102 76-77 0.20583774381069375 1102 78-79 0.1713404232156428 1102 80-81 0.2197196620378925 1102 82-83 0.027204656187208798 1102 84-85 -0.01480919736657782 1102 86-87 -0.035395667939205566 1102 88-89 -0.08068109510072574 1102 90-91 -0.04957001998871746 1102 92-93 -0.025773001098432502 1102 94-95 -0.2556731600152382 1102 96-97 -0.13927111404225556 1102 98-99 -0.1674950569570477 1102 100-101 -0.6059322577810988 1103 1 0.2593751726253757 1103 2 0.3254372942742805 1103 3 0.3984168777762065 1103 4 0.400250377344193 1103 5 0.3975910533394895 1103 6 0.3608560453563925 1103 7 0.350779942903344 1103 8 0.41572283903855833 1103 9 0.5262478957545866 1103 10-11 0.4745091031448112 1103 12-13 0.4629810176154905 1103 14-15 0.5286443963565262 1103 16-17 0.5863052348597435 1103 18-19 0.6064671027971684 1103 20-21 0.5933949657787352 1103 22-23 0.6527044547890668 1103 24-25 0.731799890578813 1103 26-27 0.6938304751881645 1103 28-29 0.6409678695644416 1103 30-31 0.8064379000515842 1103 32-33 0.774908475951058 1103 34-35 0.7630889993010115 1103 36-37 0.7055465333362889 1103 38-39 0.7062924046615962 1103 40-41 0.6758981601717338 1103 42-43 0.733975855062809 1103 44-45 0.6369873693470751 1103 46-47 0.8423124639257296 1103 48-49 0.9141991800523854 1103 50-51 0.7551850034138141 1103 52-53 0.8782654510363059 1103 54-55 0.7880710727346596 1103 56-57 0.8900982658320231 1103 58-59 0.941525033069162 1103 60-61 0.9524376895894022 1103 62-63 0.7395747830188597 1103 64-65 0.7966654967129685 1103 66-67 0.7832523399004927 1103 68-69 0.6595650823998227 1103 70-71 0.6523674288523083 1103 72-73 0.7349111256778045 1103 74-75 0.695446328983337 1103 76-77 0.6468585755817493 1103 78-79 0.6901946465147972 1103 80-81 0.6131478235365542 1103 82-83 0.5470970115048814 1103 84-85 0.42708719213680624 1103 86-87 0.498420222979604 1103 88-89 0.4910447759459373 1103 90-91 0.5479417439452163 1103 92-93 0.45816055845238424 1103 94-95 0.37148058311856147 1103 96-97 0.37193625927283236 1103 98-99 0.3599838054564799 1103 100-101 0.324674153094211 1104 1 0.1686221781661459 1104 2 0.2579985867580348 1104 3 0.2763318355866957 1104 4 0.22574334274155206 1104 5 0.2666423099712105 1104 6 0.24623451739000046 1104 7 0.29275409051230383 1104 8 0.2866447839205932 1104 9 0.2872338039154485 1104 10-11 0.3842988968598462 1104 12-13 0.40410568829287286 1104 14-15 0.46385865976029805 1104 16-17 0.3697826892751621 1104 18-19 0.3522396642254506 1104 20-21 0.3755771977486049 1104 22-23 0.5251800724670161 1104 24-25 0.41792544963506373 1104 26-27 0.5020883246096446 1104 28-29 0.5201483428836511 1104 30-31 0.46741540539317583 1104 32-33 0.47769227056239316 1104 34-35 0.5085734034420923 1104 36-37 0.44940837288034885 1104 38-39 0.3616582696370614 1104 40-41 0.3676087648125872 1104 42-43 0.45416112449623824 1104 44-45 0.44639190652997485 1104 46-47 0.6517063914883394 1104 48-49 0.6832619799509843 1104 50-51 0.5998259285953367 1104 52-53 0.5960143272653227 1104 54-55 0.5920395234019082 1104 56-57 0.6751060518787071 1104 58-59 0.6894991614393433 1104 60-61 0.6560941608353517 1104 62-63 0.7363380000594404 1104 64-65 0.7684654088936043 1104 66-67 0.5885184178605485 1104 68-69 0.6188851104205355 1104 70-71 0.6078140966959396 1104 72-73 0.634669877058478 1104 74-75 0.4894489511156337 1104 76-77 0.4542341289795466 1104 78-79 0.4202194192708717 1104 80-81 0.37659530506732963 1104 82-83 0.4154440722722619 1104 84-85 0.40574360679811505 1104 86-87 0.4405175836455797 1104 88-89 0.43060997390164957 1104 90-91 0.4718263474905804 1104 92-93 0.38037251999293176 1104 94-95 0.3849580545093687 1104 96-97 0.32751618394735615 1104 98-99 0.2450988759835795 1104 100-101 0.007886876264038278 1105 1 0.1861889627514799 1105 2 0.21383776101770025 1105 3 0.22354999000881293 1105 4 0.19645395957760314 1105 5 0.3534816252117281 1105 6 0.33844658844761 1105 7 0.2674541394888905 1105 8 0.3374035950201062 1105 9 0.3864928802708434 1105 10-11 0.3282471919286891 1105 12-13 0.31856616761147194 1105 14-15 0.30280637216261397 1105 16-17 0.3234152687276506 1105 18-19 0.34059040828653053 1105 20-21 0.3582644639179975 1105 22-23 0.3194886021345198 1105 24-25 0.38647306978865004 1105 26-27 0.4963064798466803 1105 28-29 0.35020590870663426 1105 30-31 0.4810701750687656 1105 32-33 0.5348355584477318 1105 34-35 0.47137666507077114 1105 36-37 0.4817137683723871 1105 38-39 0.3875692055950779 1105 40-41 0.39368370306215894 1105 42-43 0.2979663606717651 1105 44-45 0.26429333728189164 1105 46-47 0.2870973118325253 1105 48-49 0.39309058635119243 1105 50-51 0.3517242805862253 1105 52-53 0.45473603024881726 1105 54-55 0.4512571041215949 1105 56-57 0.43834663936954854 1105 58-59 0.3822161150794656 1105 60-61 0.30920894125156906 1105 62-63 0.3676158244233747 1105 64-65 0.3797748846811615 1105 66-67 0.31795864238682015 1105 68-69 0.26415182092979705 1105 70-71 0.2221473265346603 1105 72-73 0.3216058008538383 1105 74-75 0.29150348218316324 1105 76-77 0.3938639686769818 1105 78-79 0.40105845091985515 1105 80-81 0.44061528034270836 1105 82-83 0.3829139630813678 1105 84-85 0.19494585890610594 1105 86-87 0.23737334393575793 1105 88-89 0.20796005543421714 1105 90-91 0.16409102151922283 1105 92-93 0.32232528407329397 1105 94-95 0.41756863687437473 1105 96-97 0.5050668983454152 1105 98-99 0.5651612661224164 1105 100-101 0.14076418136208702 1106 1 0.20770015158419142 1106 2 0.22792563394919796 1106 3 0.22575721680235006 1106 4 0.3098258713737607 1106 5 0.29971391196023944 1106 6 0.3011514000757529 1106 7 0.28580705535095774 1106 8 0.3105963508128582 1106 9 0.32460043647805037 1106 10-11 0.3131855192606068 1106 12-13 0.3488318870987541 1106 14-15 0.3819196435904857 1106 16-17 0.3887687525080423 1106 18-19 0.41949258455269955 1106 20-21 0.4800511812580268 1106 22-23 0.45597030472156064 1106 24-25 0.43646106169627075 1106 26-27 0.4304874029993613 1106 28-29 0.44659551067190506 1106 30-31 0.4615031003374028 1106 32-33 0.2746534063398016 1106 34-35 0.37929485116227823 1106 36-37 0.40054642922624595 1106 38-39 0.4180711412259157 1106 40-41 0.4962021669838279 1106 42-43 0.42799506172234913 1106 44-45 0.39588466862290517 1106 46-47 0.3056129879828191 1106 48-49 0.34355174707734193 1106 50-51 0.427800280631466 1106 52-53 0.3587381051630061 1106 54-55 0.3007278405451501 1106 56-57 0.30276199257712477 1106 58-59 0.42198453645328726 1106 60-61 0.5823482590603959 1106 62-63 0.6289105992854545 1106 64-65 0.5844470321936726 1106 66-67 0.5554928483520385 1106 68-69 0.542678405636309 1106 70-71 0.6002250085019725 1106 72-73 0.6303825852787313 1106 74-75 0.5921588899151118 1106 76-77 0.6158429601708342 1106 78-79 0.5679521928492441 1106 80-81 0.5468386287565217 1106 82-83 0.6766320998778745 1106 84-85 0.7116874807956073 1106 86-87 0.6013914962783318 1106 88-89 0.807080544869855 1106 90-91 0.7768589118012947 1106 92-93 0.6473120725412116 1106 94-95 0.781518133968099 1106 96-97 0.7173635268992093 1106 98-99 0.5143691397483536 1106 100-101 0.40692670543416654 1107 1 -0.022729232116226683 1107 2 0.07756291255844161 1107 3 0.08500171062628681 1107 4 0.09875796731520126 1107 5 0.14135171341112596 1107 6 0.02903519852030456 1107 7 0.08589528487843978 1107 8 0.15205769320878204 1107 9 0.16713033648458975 1107 10-11 0.07338419908546001 1107 12-13 0.10346557117168942 1107 14-15 0.10659776920319075 1107 16-17 0.06834034913571685 1107 18-19 0.12231756331226507 1107 20-21 0.0886944071909781 1107 22-23 0.11404330648615257 1107 24-25 0.1487462064580498 1107 26-27 0.14918716118509678 1107 28-29 0.19076746021768543 1107 30-31 0.14650244678534108 1107 32-33 0.1867800647281328 1107 34-35 0.21662574456792782 1107 36-37 0.24631304578725377 1107 38-39 0.10521413841563998 1107 40-41 0.2463753582785131 1107 42-43 0.1356105770481406 1107 44-45 0.1455660619958863 1107 46-47 0.2682151054914854 1107 48-49 0.22901348160449686 1107 50-51 0.12073538291236474 1107 52-53 0.2590109631461459 1107 54-55 0.15639413686565717 1107 56-57 0.07615915616120361 1107 58-59 0.2136827914693029 1107 60-61 0.05443831853362724 1107 62-63 0.16630358013637903 1107 64-65 0.23226906996897867 1107 66-67 0.1592327999891907 1107 68-69 0.13620007088694308 1107 70-71 0.17453877884377533 1107 72-73 0.2692378888536595 1107 74-75 0.23412346739370804 1107 76-77 0.1297979304342185 1107 78-79 0.1545756161549079 1107 80-81 0.16717226708081157 1107 82-83 0.012582364566451787 1107 84-85 0.13158814088318493 1107 86-87 0.125540244726146 1107 88-89 0.11044797183541277 1107 90-91 -0.0035165538545456343 1107 92-93 -0.05849473747120726 1107 94-95 -0.007356776168492729 1107 96-97 0.07543489478366183 1107 98-99 0.019424364897041357 1107 100-101 -0.14283474806560292 1108 1 0.12250461031845816 1108 2 0.21034636423622288 1108 3 0.19159931176552192 1108 4 0.24835326355150755 1108 5 0.2238153659237625 1108 6 0.168858880786253 1108 7 0.1543325509014295 1108 8 0.21773078848299576 1108 9 0.295569462089567 1108 10-11 0.30035058469425024 1108 12-13 0.3335359906052986 1108 14-15 0.3226978342885687 1108 16-17 0.3111130592807143 1108 18-19 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2316 84-85 -1.232363787691387 2316 86-87 -1.340692291081858 2316 88-89 -1.5118757736015418 2316 90-91 -1.669052171276956 2316 92-93 -1.5516952476528516 2316 94-95 -1.4988379888761187 2316 96-97 -1.76247852827127 2316 98-99 -2.1377887312311508 2316 100-101 -1.6598889988462702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11709.0 3 3575.0 4 3503.0 5 3822.0 6 4335.0 7 5061.0 8 6200.0 9 7298.0 10 8857.0 11 10649.0 12 13257.0 13 19295.0 14 16810.0 15 16319.0 16 19248.0 17 25217.0 18 22095.0 19 17009.0 20 17544.0 21 18486.0 22 19516.0 23 21685.0 24 24191.0 25 27797.0 26 30961.0 27 35551.0 28 42004.0 29 49789.0 30 59518.0 31 73076.0 32 91578.0 33 119999.0 34 166998.0 35 248921.0 36 402736.0 37 637338.0 38 578935.0 39 94717.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49355750840168 18.48128521221204 14.545108574817823 23.480048704568464 2 15.397982900139562 19.67910509025674 40.62115182485091 24.301760184752784 3 19.80996127021319 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22-23 23.052314248106935 25.75092765361409 28.347685011855734 22.849073086423243 24-25 22.965377890712812 25.92173615524388 28.37915534447335 22.733730609569953 26-27 22.635756453809204 26.09477782478475 28.469850262711017 22.799615458695033 28-29 22.572276133888725 26.460442487581403 28.410715619341993 22.556565759187876 30-31 22.76006270759782 26.06595263696011 28.20699898464283 22.96698567079924 32-33 22.594605158506244 26.065161052583246 28.27260494020056 23.067628848709955 34-35 23.173589470265828 26.165705523686068 28.027431777170854 22.63327322887725 36-37 23.118602010189818 25.853597470757233 28.114275442700265 22.91352507635268 38-39 22.753446228897534 26.22120218084516 28.134348412630985 22.89100317762632 40-41 23.040158048706356 26.105087192735837 28.09927012229356 22.755484636264242 42-43 22.823864560546888 26.14119069444086 27.801196562227165 23.233748182785092 44-45 22.899816869710257 26.222395950964124 27.88301824258775 22.99476893673787 46-47 23.22067776448766 26.528588327642755 27.387521091563695 22.86321281630589 48-49 23.160734726312217 26.473598180415138 27.328099639299463 23.03756745397318 50-51 22.9652123322799 26.73945095717947 27.396496164322286 22.898840546218345 52-53 23.236765319234944 26.67171869802868 27.1434462140256 22.948069768710774 54-55 22.926671475168366 26.478386235297897 27.365723716104707 23.22921857342903 56-57 22.9582487029343 26.75591050601267 27.253358253566297 23.032482537486736 58-59 23.15775094884526 26.412022567641635 27.263881135919487 23.16634534759362 60-61 22.934214074693358 26.552235768310258 27.56041821151361 22.95313194548278 62-63 22.81464337267964 26.616712376750396 27.573930339284846 22.994713911285114 64-65 22.641204070127614 26.65755864310834 27.466536291396835 23.23470099536721 66-67 22.65838966020728 26.694765055858305 27.218272986450128 23.42857229748429 68-69 22.70777238880498 26.493119286983813 27.505396389159166 23.293711935052045 70-71 22.853606975072925 26.506821527100826 27.280329524511487 23.35924197331476 72-73 22.89169104322431 26.454357183670453 27.383290718706633 23.27066105439861 74-75 22.7074312538564 26.934400814228454 27.05932622054859 23.298841711366556 76-77 22.869676789031644 26.57381779337646 27.115897920291992 23.440607497299904 78-79 22.933460141216745 26.658751305975407 27.201962775289147 23.205825777518697 80-81 22.94207252713477 26.724170251141206 27.0755247518733 23.258232469850725 82-83 22.9227299928604 26.730483359595198 27.11018108439846 23.236605563145947 84-85 22.950409892687127 26.733265572425974 27.01314244079112 23.30318209409578 86-87 22.952254731061608 26.694694330191254 27.031894415922668 23.321156522824467 88-89 22.87923591176851 26.749033893375074 26.859470209727654 23.512259985128765 90-91 22.876516514466854 26.726251346703535 26.957435805512443 23.43979633331717 92-93 23.015310898074905 26.555825136094995 27.02113316189813 23.40773080393197 94-95 22.852972749779607 26.472369140857964 27.136822820348982 23.537835289013444 96-97 22.919388181961327 26.28955053013372 27.230386674244674 23.56067461366028 98-99 22.85851570791818 26.46263758134587 26.865789403435997 23.81305730729995 100-101 23.15222750557111 25.724353776014198 27.350004062309925 23.773414656104773 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2602.0 1 1453.5 2 375.5 3 465.5 4 428.5 5 422.5 6 682.5 7 1429.5 8 2376.5 9 2849.0 10 2716.0 11 2562.0 12 2748.5 13 3065.0 14 3383.5 15 3755.0 16 4063.5 17 4222.5 18 4377.0 19 4626.5 20 4956.0 21 5465.0 22 6311.5 23 7676.5 24 9537.0 25 11960.5 26 15309.0 27 19187.0 28 22622.0 29 26181.5 30 30545.0 31 35763.0 32 41692.0 33 45862.0 34 48970.5 35 52752.5 36 57738.0 37 63772.5 38 68391.0 39 72018.0 40 76629.5 41 82794.0 42 87897.5 43 93127.0 44 98820.5 45 103866.0 46 107144.5 47 112343.5 48 122484.0 49 123267.5 50 116195.5 51 112308.5 52 110351.0 53 110795.5 54 109844.0 55 102978.5 56 94262.0 57 86429.0 58 79623.0 59 70697.0 60 59099.0 61 49104.0 62 40415.0 63 31410.0 64 23524.0 65 17848.0 66 13612.0 67 10883.5 68 8585.0 69 6363.5 70 4710.5 71 3510.5 72 2672.0 73 2176.0 74 1717.0 75 1283.5 76 920.5 77 582.0 78 374.5 79 247.0 80 174.0 81 130.5 82 93.5 83 71.0 84 57.0 85 36.5 86 27.0 87 24.5 88 19.0 89 18.0 90 11.5 91 7.5 92 9.5 93 8.0 94 4.0 95 4.5 96 4.5 97 2.5 98 1.5 99 2.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3907755725611561 2 0.11537087552480642 3 0.11728644205696896 4 0.12827574479411188 5 0.13062819492132902 6 0.07379972113384063 7 0.08247018017415525 8 0.07964724002149468 9 0.07924396285682889 10-11 0.07779888635010977 12-13 0.08152920012326839 14-15 0.07549684586847585 16-17 0.07702593845116701 18-19 0.08636852609925794 20-21 0.08510828495967732 22-23 0.09403079222790806 24-25 0.10801106726965565 26-27 0.1091704891180698 28-29 0.10293649628094438 30-31 0.09327464754415969 32-33 0.09159432602471887 34-35 0.3437265700168133 36-37 0.5377028862210611 38-39 0.5172869797598552 40-41 0.4875788952961415 42-43 0.5172197668990774 44-45 0.5278393989019435 46-47 0.5246131815846171 48-49 0.5318217609030182 50-51 0.5302758651051327 52-53 0.5294861139909954 54-55 0.5237898240400911 56-57 0.49424977172832163 58-59 0.4835965332950668 60-61 0.43063279900229223 62-63 0.5079443921117642 64-65 0.536341825790314 66-67 0.5463733452613756 68-69 0.5611601746324549 70-71 0.556841748327492 72-73 0.5428110636401612 74-75 0.5462725259702093 76-77 0.5223111411029833 78-79 0.49440100066507126 80-81 0.5019456442873605 82-83 0.51658124472169 84-85 0.5112210190746739 86-87 0.5112378222898682 88-89 0.5223783539637608 90-91 0.540374597436972 92-93 0.5474823574642066 94-95 0.5418028707284966 96-97 0.5437184372606592 98-99 0.5440881079949362 100-101 0.5203115584948487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2975621.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.41717666870931 #Duplication Level Percentage of deduplicated Percentage of total 1 76.72245798368927 43.28464466523425 2 13.13877701988703 14.825054086834891 3 4.2972449422623775 7.273152812890024 4 1.9165058117034648 4.324953878619301 5 1.0271597266105363 2.8974725881584895 6 0.6422183954502525 2.173928920560715 7 0.4171812930297272 1.6475333498217095 8 0.30320491829383706 1.3684772353763983 9 0.21533735124329928 1.0933852849618615 >10 1.2081510724308555 12.371605097341778 >50 0.07513709294256808 2.8809812384938778 >100 0.03268167396067695 3.239648328677089 >500 0.002747955315723525 1.0637883209612446 >1k 0.0010752868622672085 1.0686414925699341 >5k 1.1947631802968984E-4 0.48673269949839126 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8037 0.27009488103491675 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6405 0.21524918664036852 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4476 0.15042238242034184 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3968 0.13335031578282316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.360643038881632E-5 2 0.0 0.0 0.0 0.0 3.360643038881632E-5 3 0.0 0.0 0.0 0.0 3.360643038881632E-5 4 0.0 0.0 0.0 0.0 3.360643038881632E-5 5 0.0 0.0 0.0 0.0 6.721286077763264E-5 6 6.721286077763264E-5 0.0 0.0 0.0 1.0081929116644895E-4 7 1.0081929116644895E-4 0.0 0.0 0.0 1.0081929116644895E-4 8 1.0081929116644895E-4 0.0 0.0 0.0 1.0081929116644895E-4 9 1.0081929116644895E-4 0.0 0.0 0.0 1.0081929116644895E-4 10-11 1.3442572155526527E-4 0.0 0.0 0.0 1.0081929116644895E-4 12-13 1.3442572155526527E-4 0.0 0.0 0.0 1.0081929116644895E-4 14-15 2.3524501272171423E-4 0.0 0.0 3.360643038881632E-5 1.0081929116644895E-4 16-17 2.520482279161224E-4 0.0 0.0 3.360643038881632E-5 1.0081929116644895E-4 18-19 3.19261088693755E-4 0.0 0.0 5.040964558322448E-5 1.0081929116644895E-4 20-21 3.696707342769795E-4 0.0 0.0 1.3442572155526527E-4 1.0081929116644895E-4 22-23 3.696707342769795E-4 0.0 0.0 2.6885144311053055E-4 1.1762250636085711E-4 24-25 5.040964558322448E-4 0.0 0.0 4.536868102490203E-4 1.3442572155526527E-4 26-27 5.377028862210611E-4 0.0 0.0 7.057350381651427E-4 1.3442572155526527E-4 28-29 6.553253925819182E-4 0.0 0.0 0.0020667954689122035 1.3442572155526527E-4 30-31 7.39341468553959E-4 0.0 0.0 0.006032354254792529 1.3442572155526527E-4 32-33 7.39341468553959E-4 0.0 0.0 0.01497166473821767 1.3442572155526527E-4 34-35 7.561446837483672E-4 0.0 0.0 0.027002766817413912 1.3442572155526527E-4 36-37 8.065543293315916E-4 0.0 0.0 0.04758670543056391 1.3442572155526527E-4 38-39 8.40160759720408E-4 0.0 0.0 0.07902552105930158 1.3442572155526527E-4 40-41 8.905704053036325E-4 0.0 0.0 0.11316965433433895 1.3442572155526527E-4 42-43 9.745864812756732E-4 0.0 0.0 0.14744821333093158 1.3442572155526527E-4 44-45 0.0010249961268588978 0.0 0.0 0.19266566541908395 1.3442572155526527E-4 46-47 0.0011426186332197546 0.0 0.0 0.2487884041684072 1.3442572155526527E-4 48-49 0.0012098314939973875 0.0 0.0 0.310573826438246 1.3442572155526527E-4 50-51 0.0012098314939973875 0.0 0.0 0.37803873544379474 1.3442572155526527E-4 52-53 0.0012098314939973875 0.0 0.0 0.44273111394226616 1.3442572155526527E-4 54-55 0.0012098314939973875 0.0 0.0 0.5213029481913187 1.3442572155526527E-4 56-57 0.0012434379243862037 0.0 0.0 0.619131267053163 1.3442572155526527E-4 58-59 0.0012434379243862037 0.0 0.0 0.7271591375380131 1.3442572155526527E-4 60-61 0.0012434379243862037 0.0 0.0 0.8436726316960392 1.3442572155526527E-4 62-63 0.0012434379243862037 0.0 0.0 0.9656975804378314 1.680321519440816E-4 64-65 0.0012434379243862037 0.0 0.0 1.0960401207008554 1.680321519440816E-4 66-67 0.0013442572155526527 1.680321519440816E-5 0.0 1.248680527526859 1.680321519440816E-4 68-69 0.0013442572155526527 3.360643038881632E-5 0.0 1.4289454201324698 1.680321519440816E-4 70-71 0.0013610604307470607 3.360643038881632E-5 0.0 1.6276434398063464 2.016385823328979E-4 72-73 0.001377863645941469 3.360643038881632E-5 0.0 1.8299709539622149 2.016385823328979E-4 74-75 0.001377863645941469 3.360643038881632E-5 0.0 2.0446656345011682 2.016385823328979E-4 76-77 0.0013946668611358772 3.360643038881632E-5 0.0 2.2842458767430394 2.016385823328979E-4 78-79 0.0014114700763302854 3.360643038881632E-5 0.0 2.5563571436012853 2.016385823328979E-4 80-81 0.001478682937107918 3.360643038881632E-5 0.0 2.8617555797596532 2.016385823328979E-4 82-83 0.0015122893674967342 3.360643038881632E-5 0.0 3.172480635134649 2.016385823328979E-4 84-85 0.0015122893674967342 3.360643038881632E-5 0.0 3.4935564710694003 2.016385823328979E-4 86-87 0.0015122893674967342 3.360643038881632E-5 0.0 3.832258879743086 2.016385823328979E-4 88-89 0.0015122893674967342 3.360643038881632E-5 0.0 4.209625486579104 2.016385823328979E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14180 0.0 26.489548 1 GGTATCA 10440 0.0 24.880615 1 GTACATG 10945 0.0 22.300861 1 ATCAACG 16595 0.0 22.204578 3 TCAACGC 16670 0.0 22.13309 4 TATCAAC 16905 0.0 22.049551 2 CAACGCA 16940 0.0 21.80791 5 TACATGG 10985 0.0 21.601198 2 AACGCAG 17195 0.0 21.529814 6 ACATGGG 10955 0.0 21.098309 3 GAGTACT 8540 0.0 20.539234 12-13 GGACCGA 550 0.0 19.797064 6 AGAGTAC 14230 0.0 19.77818 10-11 GTACTTT 9020 0.0 19.289263 14-15 ACGCAGA 19135 0.0 19.198572 7 CGCAGAG 19320 0.0 18.965727 8 AGGACCG 810 0.0 18.711037 5 GTGTAGC 1780 0.0 18.674644 1 AGTACTT 9175 0.0 18.343721 12-13 CATGGGG 6820 0.0 18.125765 4 >>END_MODULE