FastQCFastQC Report
Fri 27 May 2016
SRR939098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975621
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82750.278093211467455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68840.23134666679661153No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48270.16221823948681635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.1479019001411806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140600.027.0101551
GGTATCA101950.026.126341
ATCAACG164950.022.6968273
TCAACGC165900.022.6240624
CAACGCA167350.022.3996835
TATCAAC168650.022.395832
AACGCAG170800.021.9472316
GTACATG98250.021.7754841
TACATGG97050.021.659512
GAGTACT89400.021.17969312-13
GTATAGG7050.020.9520551
ACATGGG99300.020.213043
ACGCAGA189800.019.6751947
GTACTTT95100.019.63579614-15
CGCAGAG193000.019.3243878
AGAGTAC144950.019.2011310-11
AGTACTT96000.018.66078812-13
CATGGGG61450.018.3778844
GCAGAGT208600.017.7660889
CTATCGT1650.001474822917.2547154
Produced by FastQC (version 0.11.5)