##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939098_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2975621 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50457938023693 34.0 31.0 34.0 31.0 34.0 2 32.72535077551879 34.0 31.0 34.0 31.0 34.0 3 32.836346093806974 34.0 31.0 34.0 31.0 34.0 4 36.279254985765995 37.0 37.0 37.0 35.0 37.0 5 36.23661279443854 37.0 37.0 37.0 35.0 37.0 6 36.275846957660264 37.0 37.0 37.0 35.0 37.0 7 36.23420859040852 37.0 37.0 37.0 35.0 37.0 8 36.20512928225738 37.0 37.0 37.0 35.0 37.0 9 37.95773520888581 39.0 38.0 39.0 35.0 39.0 10-11 37.867330718529004 39.0 38.0 39.0 35.0 39.0 12-13 37.7787238361337 39.0 38.0 39.0 35.0 39.0 14-15 39.20464770210991 40.5 38.5 41.0 36.0 41.0 16-17 39.306780500608106 41.0 39.0 41.0 36.0 41.0 18-19 39.32463374872002 41.0 39.0 41.0 36.0 41.0 20-21 39.28330388849924 41.0 39.0 41.0 36.0 41.0 22-23 39.21166170019636 41.0 39.0 41.0 36.0 41.0 24-25 39.131896669636355 41.0 39.0 41.0 35.5 41.0 26-27 38.98101169470171 40.0 38.5 41.0 35.0 41.0 28-29 38.82727034121617 40.0 38.0 41.0 35.0 41.0 30-31 38.63533527959374 40.0 38.0 41.0 34.5 41.0 32-33 38.54746202557382 40.0 38.0 41.0 34.0 41.0 34-35 38.43541768256105 40.0 38.0 41.0 34.0 41.0 36-37 38.284856001486745 40.0 38.0 41.0 34.0 41.0 38-39 38.146123111780696 40.0 38.0 41.0 34.0 41.0 40-41 37.95285723551487 40.0 38.0 41.0 33.0 41.0 42-43 37.75584978732171 40.0 37.0 41.0 33.0 41.0 44-45 37.49662322587453 40.0 37.0 41.0 32.5 41.0 46-47 37.190838819863146 40.0 37.0 41.0 31.5 41.0 48-49 36.84435685861875 40.0 36.0 41.0 31.0 41.0 50-51 36.56949372920812 39.0 36.0 41.0 30.5 41.0 52-53 36.27027887624129 39.0 35.0 40.5 30.0 41.0 54-55 35.988326806404444 39.0 35.0 40.0 29.0 41.0 56-57 35.69415157373872 38.5 35.0 40.0 28.5 41.0 58-59 35.31005175054216 38.0 34.5 40.0 28.0 41.0 60-61 34.895544997161934 38.0 34.0 40.0 27.0 41.0 62-63 34.45801834306184 37.0 34.0 40.0 26.0 41.0 64-65 33.96356205981878 36.5 33.5 39.0 26.0 41.0 66-67 33.50272951427618 36.0 33.0 39.0 25.0 40.0 68-69 32.97014875214283 35.5 32.5 38.5 23.5 40.0 70-71 32.426213553406164 35.0 32.0 38.0 22.0 40.0 72-73 31.81697198668782 35.0 31.0 37.0 20.5 39.0 74-75 31.24290677475391 34.5 31.0 36.5 18.5 39.0 76-77 29.271391249087166 32.5 28.5 34.5 13.5 36.5 78-79 30.252441927248128 34.0 30.0 35.0 15.0 37.0 80-81 30.259561113461693 34.0 30.0 35.0 12.0 37.0 82-83 29.851269701349736 34.0 30.0 35.0 7.5 36.5 84-85 29.34259924230942 34.0 29.0 35.0 4.0 36.0 86-87 28.59635669327512 33.5 29.0 35.0 2.0 36.0 88-89 27.877276205538273 33.0 27.0 35.0 2.0 35.0 90-91 27.26858897688919 33.0 26.0 35.0 2.0 35.0 92-93 26.759869788524817 32.0 25.0 34.0 2.0 35.0 94-95 26.148290558508627 32.0 24.5 34.0 2.0 35.0 96-97 25.25044100038278 32.0 21.5 34.0 2.0 35.0 98-99 23.92230378129473 31.0 4.5 34.0 2.0 35.0 100-101 18.002246085775035 24.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20851854525893998 1101 2 0.18716445498511547 1101 3 0.2596179808160457 1101 4 0.17649049176172582 1101 5 0.21909911755795264 1101 6 0.1817724243191421 1101 7 0.2580773467561386 1101 8 0.21397312110155298 1101 9 0.24490415036407143 1101 10-11 0.2559033595122173 1101 12-13 0.2655738804787333 1101 14-15 0.35934241712669746 1101 16-17 0.34935308234112483 1101 18-19 0.3250074371027907 1101 20-21 0.33699653739070357 1101 22-23 0.351346897514361 1101 24-25 0.36165757267354337 1101 26-27 0.33870343339559383 1101 28-29 0.40742537777471455 1101 30-31 0.40687448087349054 1101 32-33 0.42203566884767696 1101 34-35 0.4157550764334488 1101 36-37 0.402871130978923 1101 38-39 0.4201772574404643 1101 40-41 0.4267124607908954 1101 42-43 0.4555512819503136 1101 44-45 0.4514318924391034 1101 46-47 0.40083326730223945 1101 48-49 0.4738241191121588 1101 50-51 0.44329964411529943 1101 52-53 0.5818038513636736 1101 54-55 0.46786207450584527 1101 56-57 0.4419826935982414 1101 58-59 0.3775761411647025 1101 60-61 0.3813607439708804 1101 62-63 0.2611732740170183 1101 64-65 0.2919602896405138 1101 66-67 0.36201364279007464 1101 68-69 0.36575300590241255 1101 70-71 0.5215281119861146 1101 72-73 0.5813279047382558 1101 74-75 0.3956997319631057 1101 76-77 0.515212860708214 1101 78-79 0.6421079368553535 1101 80-81 0.6424442324463548 1101 82-83 0.614554205324982 1101 84-85 0.60689901630872 1101 86-87 0.6983055194310985 1101 88-89 0.7446001277944596 1101 90-91 0.8549889950450051 1101 92-93 0.849719406913021 1101 94-95 0.8109590795880308 1101 96-97 0.7111532907154547 1101 98-99 0.5313568797186257 1101 100-101 -0.29605837113865974 1102 1 0.12845756415228493 1102 2 0.0902014113624503 1102 3 0.10930625479332434 1102 4 0.12474373475619416 1102 5 0.1869289445666169 1102 6 0.12847504386156317 1102 7 0.1719630329804147 1102 8 0.19042997765064484 1102 9 0.1683643210779593 1102 10-11 0.20501702207947403 1102 12-13 0.2105718224701647 1102 14-15 0.2465937954586579 1102 16-17 0.26026859367618016 1102 18-19 0.16045675315596952 1102 20-21 0.1710093990545758 1102 22-23 0.14291896695762318 1102 24-25 0.17769607226075834 1102 26-27 0.21518718639661216 1102 28-29 0.19201119020770108 1102 30-31 0.13604055445411944 1102 32-33 0.12514865885536608 1102 34-35 0.18614967436855778 1102 36-37 0.2263659082632543 1102 38-39 0.1924643929297858 1102 40-41 0.1972384272839065 1102 42-43 0.1278875546180629 1102 44-45 0.2346696203027534 1102 46-47 0.20353074123017478 1102 48-49 0.11618448886571997 1102 50-51 -0.015401402562943645 1102 52-53 -0.07382592890981243 1102 54-55 -0.0974322503724494 1102 56-57 -0.07046188251130303 1102 58-59 -0.28280025495983097 1102 60-61 -0.2931981186324393 1102 62-63 -0.33361195766611473 1102 64-65 -0.01605701779630664 1102 66-67 0.029389047274761992 1102 68-69 -0.06840554734722559 1102 70-71 -0.13636503288819313 1102 72-73 -0.07850848654670628 1102 74-75 -0.14055413495787406 1102 76-77 -0.07159195348978997 1102 78-79 -0.11692520835109121 1102 80-81 0.2240964633988014 1102 82-83 0.12696972021855757 1102 84-85 0.03350283957857059 1102 86-87 0.05651184338892534 1102 88-89 -0.09890626211453579 1102 90-91 0.1818943136033795 1102 92-93 -0.1392490444681549 1102 94-95 -0.2291465846208851 1102 96-97 -0.18053526082609395 1102 98-99 -0.2367239413022446 1102 100-101 -0.8571652120302815 1103 1 0.12489821252783173 1103 2 0.08626769948978108 1103 3 0.09134223555859222 1103 4 0.1064202945319721 1103 5 0.11084388570007775 1103 6 0.14786415120863694 1103 7 0.07163595109218335 1103 8 0.09559292688294363 1103 9 0.19044483840984583 1103 10-11 0.15072043268294522 1103 12-13 0.15716338665156826 1103 14-15 0.07378977861420566 1103 16-17 0.18405083904091413 1103 18-19 0.22484935218860613 1103 20-21 0.27260045940214894 1103 22-23 0.23410058616236284 1103 24-25 0.22101845794906438 1103 26-27 0.24844212279636935 1103 28-29 0.22963395576326917 1103 30-31 0.23680146069884245 1103 32-33 0.3250463395793801 1103 34-35 0.28424348353974693 1103 36-37 0.20612833889821758 1103 38-39 0.353969789810229 1103 40-41 0.32644851544310427 1103 42-43 0.3030134544887133 1103 44-45 0.2913520971967074 1103 46-47 0.31340195896137146 1103 48-49 0.18268440215943116 1103 50-51 0.19359370087531147 1103 52-53 0.18655392664220471 1103 54-55 0.0998488631237393 1103 56-57 0.11708996550429163 1103 58-59 0.04378878419391441 1103 60-61 0.025702621417430294 1103 62-63 0.022446233968835827 1103 64-65 0.23423341896903338 1103 66-67 0.09952681653292927 1103 68-69 -0.032790354531194055 1103 70-71 -0.08269501357847275 1103 72-73 -0.02406909541230462 1103 74-75 -0.006115892095913011 1103 76-77 0.11038374297259423 1103 78-79 0.06706465170027798 1103 80-81 0.11899497017595095 1103 82-83 0.09392788795069507 1103 84-85 -0.2381565957057319 1103 86-87 -0.2712184379612559 1103 88-89 -0.02360551433140401 1103 90-91 0.12821477417779548 1103 92-93 0.12363445509128113 1103 94-95 0.05595305730558664 1103 96-97 -0.20750355210297045 1103 98-99 -0.35341756698417726 1103 100-101 -1.457453188211833 1104 1 0.08021473569584714 1104 2 0.12115184809463386 1104 3 0.12697754528028327 1104 4 0.05274155722956664 1104 5 0.08435180528651642 1104 6 0.041559433860236084 1104 7 0.1033971933287674 1104 8 0.08500339413627955 1104 9 0.12241057754106777 1104 10-11 0.13402371957160852 1104 12-13 0.17824031686686226 1104 14-15 0.133324036087906 1104 16-17 0.18831449178323112 1104 18-19 0.24967671244717593 1104 20-21 0.24558412228443416 1104 22-23 0.1562984015425215 1104 24-25 0.1709701912498076 1104 26-27 0.20077819143128295 1104 28-29 0.19767084781788213 1104 30-31 0.1720041242510959 1104 32-33 0.06531703484619555 1104 34-35 0.08980139678869392 1104 36-37 0.2278083199310501 1104 38-39 0.17118300752982663 1104 40-41 0.18776196380170518 1104 42-43 0.191881501367952 1104 44-45 0.13604309978602203 1104 46-47 0.03925644363372527 1104 48-49 0.17278520203992542 1104 50-51 0.21148477430859458 1104 52-53 -0.030399793911144002 1104 54-55 0.012985496926042117 1104 56-57 5.632326562334811E-4 1104 58-59 -0.06431461744840306 1104 60-61 -0.027199774602635785 1104 62-63 -0.0023152051823061015 1104 64-65 -0.13276225909604733 1104 66-67 -0.1746195475643475 1104 68-69 -0.2805133885544038 1104 70-71 -0.34218652135545824 1104 72-73 -0.3547603503691228 1104 74-75 -0.5394124171199053 1104 76-77 -0.44073906607008695 1104 78-79 -0.18804918528163483 1104 80-81 -0.16390956474356244 1104 82-83 -0.25125398640424024 1104 84-85 -0.364060110970005 1104 86-87 -0.2614795138389958 1104 88-89 -0.16423320210187953 1104 90-91 -0.16479648503418431 1104 92-93 -0.2316904991883284 1104 94-95 -0.1252400185449929 1104 96-97 -0.45190195551072776 1104 98-99 -0.6367842569637148 1104 100-101 -1.025457034835764 1105 1 -0.02682477235432401 1105 2 0.05467285709377023 1105 3 0.07349772224792162 1105 4 0.08410286996502947 1105 5 0.017355341216664044 1105 6 0.09798722354940281 1105 7 0.07934365972113255 1105 8 0.06100731226307943 1105 9 0.06290321190274284 1105 10-11 0.06405260027328552 1105 12-13 0.07579103941409926 1105 14-15 0.09871527425019622 1105 16-17 0.10004845847100086 1105 18-19 0.20008303067111655 1105 20-21 0.21470625978232505 1105 22-23 0.17637594916022437 1105 24-25 0.055266676426036554 1105 26-27 0.10881365178266122 1105 28-29 0.10561096273765003 1105 30-31 0.21550629234101137 1105 32-33 0.16791931319455244 1105 34-35 0.2600803921901118 1105 36-37 0.22268126195695714 1105 38-39 0.2843466259948926 1105 40-41 0.3380958357959756 1105 42-43 0.28800925446729053 1105 44-45 0.35054729946705976 1105 46-47 0.3708389251737927 1105 48-49 0.28686682587856893 1105 50-51 0.31080211731664065 1105 52-53 0.32872060781093637 1105 54-55 0.33868854724643427 1105 56-57 0.30897168107638606 1105 58-59 0.08854529729485705 1105 60-61 0.0984663191765236 1105 62-63 0.11056971125190529 1105 64-65 0.21063918510201773 1105 66-67 0.2420913284156967 1105 68-69 0.20790601236661388 1105 70-71 -0.03014231530175948 1105 72-73 -0.12575569963084732 1105 74-75 -0.1000335722338832 1105 76-77 -0.12820550162594557 1105 78-79 -0.3562491205771252 1105 80-81 0.01107700947878243 1105 82-83 -0.12337009269239374 1105 84-85 -0.13685276236347121 1105 86-87 -0.07304638154598919 1105 88-89 -0.12383424258946363 1105 90-91 -0.24569973223880837 1105 92-93 -0.16375382322344478 1105 94-95 -0.29844620018863566 1105 96-97 -0.46976435167995945 1105 98-99 -0.5676745062227511 1105 100-101 -1.053376336744222 1106 1 -0.002500428123745735 1106 2 0.04925685231894761 1106 3 0.03743569788609591 1106 4 0.05885707233702675 1106 5 0.08666597149552757 1106 6 0.05707224636740449 1106 7 0.10591363559710487 1106 8 0.06698321242940608 1106 9 0.014143517636931335 1106 10-11 0.06333730990761666 1106 12-13 0.06313256350275509 1106 14-15 0.08451626399057233 1106 16-17 0.043760361055255714 1106 18-19 0.048387482766713674 1106 20-21 0.15095335081208106 1106 22-23 0.1632591797342755 1106 24-25 0.18042512932077415 1106 26-27 0.1405020605933629 1106 28-29 0.10579108412328964 1106 30-31 0.0869651874439441 1106 32-33 0.09834767459893357 1106 34-35 0.09953226288781991 1106 36-37 0.11672260451692296 1106 38-39 0.26824749623305166 1106 40-41 0.20646182509049282 1106 42-43 0.11998717272967241 1106 44-45 0.21085201829225042 1106 46-47 0.22659089348821482 1106 48-49 0.08597795084348547 1106 50-51 0.20578622007539593 1106 52-53 0.24624487323265498 1106 54-55 0.14794085475986662 1106 56-57 0.038052084790294316 1106 58-59 -0.004231289318738618 1106 60-61 0.06150414821863848 1106 62-63 0.07408043291327004 1106 64-65 0.08086767818770113 1106 66-67 0.1002254497324131 1106 68-69 -0.0064362664036821116 1106 70-71 0.06073684804064783 1106 72-73 -0.15195445052619405 1106 74-75 -0.2557447945787743 1106 76-77 -0.21769742121881208 1106 78-79 -0.03081824312884862 1106 80-81 0.14000821297372923 1106 82-83 -0.10945793511044855 1106 84-85 -0.3117063701704943 1106 86-87 -0.20482020169501425 1106 88-89 -0.22548215269475236 1106 90-91 0.09368243102231943 1106 92-93 0.14155179390701633 1106 94-95 -0.04121339602681218 1106 96-97 -0.56321716619005 1106 98-99 -0.5367163156910166 1106 100-101 -0.9109038060775525 1107 1 -0.08453754988049411 1107 2 -0.047514600527271966 1107 3 -0.07002459882653511 1107 4 -0.06354015660225798 1107 5 -0.049132342340172386 1107 6 0.021486336949720908 1107 7 0.01826576944456093 1107 8 -0.003910846782410715 1107 9 -0.03299719212060381 1107 10-11 0.019823814057673417 1107 12-13 -0.048234340621156946 1107 14-15 -0.023478311527362905 1107 16-17 -0.10210918995448282 1107 18-19 0.01766073288109027 1107 20-21 0.00903125421708495 1107 22-23 -0.05345378020297886 1107 24-25 -0.11334161795064546 1107 26-27 -0.06630795182412186 1107 28-29 -0.056458115275447085 1107 30-31 -0.06988240421672742 1107 32-33 -0.09929300248099793 1107 34-35 -0.1074656457456058 1107 36-37 -0.028508099358639072 1107 38-39 -0.12354225607071356 1107 40-41 -0.12229119758776363 1107 42-43 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-0.07264370406239706 2316 84-85 -0.01120021499805901 2316 86-87 0.042532313048614157 2316 88-89 -0.21930550346572986 2316 90-91 -0.35429428587742606 2316 92-93 -0.32672698838164393 2316 94-95 -0.04804103688064032 2316 96-97 0.05398609993977388 2316 98-99 0.4178436022902545 2316 100-101 1.4505349202659517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 24.0 5 54.0 6 116.0 7 252.0 8 698.0 9 1382.0 10 2259.0 11 3539.0 12 5393.0 13 7851.0 14 10044.0 15 13600.0 16 17444.0 17 24172.0 18 24706.0 19 19435.0 20 18640.0 21 19902.0 22 21300.0 23 23127.0 24 26237.0 25 30075.0 26 34641.0 27 41544.0 28 50240.0 29 60801.0 30 73184.0 31 89851.0 32 112558.0 33 146404.0 34 204193.0 35 303762.0 36 483765.0 37 695746.0 38 393221.0 39 15446.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79566357724164 18.990530570862425 14.915000592324487 23.298805259571438 2 15.76064290445591 20.167689366354118 39.83353390771204 24.238133821477938 3 19.874943751237137 23.678015446187537 29.204391285046043 27.242649517529284 4 13.67472537665247 16.621068341700774 35.943287132333055 33.76091914931371 5 14.45778881114228 36.62311161266841 33.631500785886374 15.287598790302932 6 32.893638000269526 35.27136016313905 17.02236272697363 14.81263910961779 7 29.32974327039633 31.901172898027003 20.18378684651036 18.58529698506631 8 26.26120732445429 35.2601020089588 19.241227293395227 19.23746337319168 9 26.06941542622532 17.45050865012715 20.17209180873505 36.307984114912486 10-11 25.481101255838695 26.23781724890367 26.592247466999325 21.688834028258302 12-13 26.31818702717853 23.393016113275177 27.332261064161063 22.956535795385232 14-15 22.863971735434067 25.03077952217008 25.668399147263987 26.43684959513187 16-17 22.669879077533075 28.15595338397059 26.345359485646817 22.828808052849514 18-19 22.62779769332183 26.751105063447262 28.476190348165982 22.144906895064928 20-21 23.242341682626922 25.859509662016766 28.58818377743671 22.3099648779196 22-23 22.9779598947581 25.782500526780794 28.498589034020128 22.740950544440974 24-25 22.93720300307163 25.93894717739496 28.476636801742156 22.647213017791252 26-27 22.559329755122608 26.160544941967085 28.59481712291626 22.68530817999405 28-29 22.529452260461305 26.515982128769444 28.490125000819155 22.464440609950092 30-31 22.692086794655637 26.09215017638335 28.32605362040394 22.88970940855707 32-33 22.550351674490805 26.127621763658748 28.383352584216876 22.938673977633574 34-35 23.11319803471713 26.194371826732983 28.142546868393403 22.549883270156485 36-37 23.10575870330262 25.879423252305433 28.252307115155578 22.762510929236367 38-39 22.694122297568477 26.253308396688947 28.30688408695646 22.745685218786114 40-41 22.97201510408002 26.180018854892946 28.18188081758311 22.666085223443925 42-43 22.783392147112703 26.18841841039622 27.890671068611557 23.137518373879523 44-45 22.905965757426507 26.231097125817065 27.969002924376067 22.893934192380357 46-47 23.20019183614209 26.577422082531214 27.44972535302152 22.772660728305176 48-49 23.157908356766235 26.510519012326263 27.376755031142487 22.954817599765015 50-51 22.96385602903616 26.787084957655598 27.410034950934264 22.839024062373976 52-53 23.291272645273036 26.70178762685167 27.109652069265543 22.89728765860975 54-55 22.964181258298687 26.548844762152168 27.302250521823844 23.184723457725294 56-57 22.975408305869855 26.806023688856616 27.257408837860098 22.96115916741343 58-59 23.179798018496964 26.431646110097578 27.294707786190177 23.093848085215285 60-61 22.990689257736786 26.545369977195747 27.590305831728447 22.873634933339023 62-63 22.836085211808747 26.636848325205207 27.551898818832736 22.975167644153306 64-65 22.640005027665605 26.707692328373778 27.412607342004936 23.23969530195568 66-67 22.6688000505452 26.6860344853371 27.279134023591233 23.366031440526463 68-69 22.691965213685656 26.4923598054163 27.522428730418497 23.293246250479545 70-71 22.838619438566575 26.53051108249705 27.227879408478685 23.40299007045769 72-73 22.890213505012902 26.477884784386184 27.27091252548628 23.360989185114637 74-75 22.696506040251766 26.95958255436428 26.971428821076337 23.372482584307612 76-77 22.80119565977922 26.544713450701572 27.15024664015027 23.503844249368935 78-79 22.827250401367465 26.65878244782643 27.191395770587718 23.32257138021839 80-81 22.879312624858876 26.770817877816295 27.042711753676013 23.307157743648823 82-83 22.854422656648815 26.71378512250048 27.12882453780236 23.302967683048344 84-85 22.762761117763315 26.795566370851663 27.00485041609802 23.436822095287 86-87 22.81626807490051 26.686999124956305 27.040498610056506 23.456234190086672 88-89 22.714663257671504 26.70347108018147 26.843002071113055 23.738863591033972 90-91 22.716763581278254 26.662776469889423 26.938645195660417 23.68181475317191 92-93 22.847634157710274 26.487496223477386 27.009656135643617 23.655213483168723 94-95 22.633447376774022 26.39111741567851 27.109001299056786 23.866433908490684 96-97 22.644998797729347 26.23562925300093 27.231707548629792 23.887664400639935 98-99 22.479349230436526 26.42891865565292 26.896317753419417 24.19541436049113 100-101 22.92378705542729 25.143936873447796 27.692456336683907 24.239819734441006 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 474.0 1 352.0 2 287.0 3 369.5 4 340.0 5 333.5 6 569.0 7 1241.0 8 2073.0 9 2462.5 10 2286.0 11 2087.5 12 2168.0 13 2387.0 14 2685.5 15 2901.0 16 3076.5 17 3291.5 18 3565.5 19 3858.5 20 4323.0 21 5060.0 22 6118.5 23 7612.5 24 9586.0 25 11932.0 26 15089.0 27 18990.5 28 22454.0 29 26079.0 30 30449.0 31 35797.5 32 42168.5 33 46319.0 34 49402.0 35 53100.0 36 58158.0 37 64232.0 38 68719.0 39 71933.0 40 76227.0 41 82972.0 42 88689.0 43 93895.0 44 99426.5 45 104806.5 46 108013.0 47 113678.0 48 124049.5 49 124718.5 50 117956.0 51 113427.5 52 111276.5 53 112017.5 54 110865.0 55 103777.0 56 94974.5 57 87102.0 58 80055.0 59 70873.0 60 58878.5 61 48481.0 62 39754.5 63 30819.0 64 22907.0 65 17171.0 66 12957.0 67 10498.0 68 8314.0 69 6010.5 70 4392.5 71 3261.5 72 2462.5 73 2015.5 74 1585.0 75 1191.5 76 820.0 77 473.0 78 288.5 79 185.0 80 102.5 81 60.5 82 35.0 83 19.5 84 16.0 85 11.0 86 5.5 87 3.5 88 2.5 89 1.0 90 2.0 91 2.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.427406581684966 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.561446837483672E-4 16-17 0.0026213015703276726 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.360643038881632E-5 26-27 0.0037471169883530196 28-29 1.680321519440816E-5 30-31 0.0 32-33 0.0 34-35 0.01260241139580612 36-37 0.02695235717183069 38-39 0.020667954689122036 40-41 0.00893931048342514 42-43 0.009056932989785996 44-45 0.0038479362795194685 46-47 0.005545061014154693 48-49 0.0034950687604368975 50-51 7.057350381651427E-4 52-53 0.0 54-55 0.0 56-57 8.40160759720408E-5 58-59 0.0017307311650240403 60-61 0.002671711215910897 62-63 0.001596305443468775 64-65 0.002856546583049387 66-67 0.002234827620856285 68-69 0.0047217034696286925 70-71 1.8483536713848975E-4 72-73 0.0 74-75 0.0 76-77 0.09641684878551401 78-79 0.016114283371437426 80-81 3.8647394947138766E-4 82-83 0.0 84-85 0.0 86-87 0.10747336438343459 88-89 0.41940825125242764 90-91 0.42402913543088994 92-93 0.0 94-95 1.680321519440816E-5 96-97 5.040964558322447E-5 98-99 1.8483536713848975E-4 100-101 3.696707342769795E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2975621.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.73374706740742 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2972100411126 41.212984492717084 2 13.902959728528092 15.21922162539216 3 4.580232355047589 7.520798376933918 4 2.0191747174197157 4.420679930726183 5 1.0917167527631406 2.9876874307494554 6 0.6671944643709519 2.1910831834592437 7 0.4510693770486885 1.7282102035265166 8 0.31436338493527055 1.376502879864091 9 0.2451420206846558 1.2075787220172398 >10 1.3083876708798605 12.936943857706792 >50 0.0839935272911286 3.1284013816370067 >100 0.03444236732059325 3.349261171423232 >500 0.0027014637956962557 1.0133199348306943 >1k 0.0012893349930746545 1.1979112459357038 >5k 1.2279380886425281E-4 0.5094155630807454 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8275 0.278093211467455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6884 0.23134666679661153 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4827 0.16221823948681635 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4401 0.1479019001411806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.360643038881632E-5 0.0 0.0 0.0 0.0 12-13 3.360643038881632E-5 0.0 0.0 0.0 0.0 14-15 1.0081929116644895E-4 0.0 0.0 3.360643038881632E-5 0.0 16-17 1.0081929116644895E-4 0.0 0.0 3.360643038881632E-5 0.0 18-19 1.1762250636085711E-4 0.0 0.0 5.040964558322448E-5 0.0 20-21 1.3442572155526527E-4 0.0 0.0 1.3442572155526527E-4 0.0 22-23 1.3442572155526527E-4 0.0 0.0 2.856546583049387E-4 1.680321519440816E-5 24-25 2.016385823328979E-4 0.0 0.0 4.872932406378366E-4 3.360643038881632E-5 26-27 2.016385823328979E-4 0.0 0.0 7.39341468553959E-4 3.360643038881632E-5 28-29 2.520482279161224E-4 0.0 0.0 0.0020835986841066115 3.360643038881632E-5 30-31 3.0245787349934686E-4 0.0 0.0 0.006049157469986937 3.360643038881632E-5 32-33 3.19261088693755E-4 0.0 0.0 0.015038877598995302 3.360643038881632E-5 34-35 3.696707342769795E-4 0.0 0.0 0.02727161826052444 3.360643038881632E-5 36-37 4.3688359505461214E-4 0.0 0.0 0.04822522760795142 3.360643038881632E-5 38-39 4.7049002544342845E-4 0.0 0.0 0.0801345332621325 3.360643038881632E-5 40-41 4.7049002544342845E-4 0.0 0.0 0.11461473084105805 3.360643038881632E-5 42-43 5.040964558322448E-4 0.0 0.0 0.1490781252047892 3.360643038881632E-5 44-45 6.3852217738751E-4 0.0 0.0 0.1944636094448856 3.360643038881632E-5 46-47 7.39341468553959E-4 0.0 0.0 0.25073757713095857 3.360643038881632E-5 48-49 8.065543293315916E-4 0.0 0.0 0.3132287344389625 3.360643038881632E-5 50-51 8.065543293315916E-4 0.0 0.0 0.3811641334699547 3.360643038881632E-5 52-53 8.065543293315916E-4 0.0 0.0 0.4461085601963422 5.040964558322448E-5 54-55 8.40160759720408E-4 0.0 0.0 0.5251508844708381 6.721286077763264E-5 56-57 8.40160759720408E-4 0.0 0.0 0.6239201833835694 6.721286077763264E-5 58-59 8.40160759720408E-4 0.0 0.0 0.7329562467800839 6.721286077763264E-5 60-61 8.40160759720408E-4 0.0 0.0 0.8502090824066639 6.721286077763264E-5 62-63 8.40160759720408E-4 0.0 0.0 0.9733262401360926 6.721286077763264E-5 64-65 8.40160759720408E-4 0.0 0.0 1.1048450054627252 6.721286077763264E-5 66-67 9.409800508868569E-4 0.0 0.0 1.258476801985199 6.721286077763264E-5 68-69 9.745864812756732E-4 0.0 0.0 1.4398002971480575 6.721286077763264E-5 70-71 0.0010754057724421222 0.0 0.0 1.6398257708222923 6.721286077763264E-5 72-73 0.0011426186332197548 0.0 0.0 1.8432622971809918 6.721286077763264E-5 74-75 0.0011426186332197548 0.0 0.0 2.0595364799482194 6.721286077763264E-5 76-77 0.0011426186332197548 0.0 0.0 2.300595405127199 6.721286077763264E-5 78-79 0.0011426186332197548 0.0 0.0 2.5732107684412764 6.721286077763264E-5 80-81 0.001176225063608571 0.0 0.0 2.8790460881946993 6.721286077763264E-5 82-83 0.001176225063608571 0.0 0.0 3.1897039307089177 8.40160759720408E-5 84-85 0.001176225063608571 3.360643038881632E-5 0.0 3.510914192365224 1.0081929116644895E-4 86-87 0.001176225063608571 3.360643038881632E-5 0.0 3.8488436531399666 1.0081929116644895E-4 88-89 0.001176225063608571 3.360643038881632E-5 0.0 4.224681167393294 1.0081929116644895E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14060 0.0 27.010155 1 GGTATCA 10195 0.0 26.12634 1 ATCAACG 16495 0.0 22.696827 3 TCAACGC 16590 0.0 22.624062 4 CAACGCA 16735 0.0 22.399683 5 TATCAAC 16865 0.0 22.39583 2 AACGCAG 17080 0.0 21.947231 6 GTACATG 9825 0.0 21.775484 1 TACATGG 9705 0.0 21.65951 2 GAGTACT 8940 0.0 21.179693 12-13 GTATAGG 705 0.0 20.952055 1 ACATGGG 9930 0.0 20.21304 3 ACGCAGA 18980 0.0 19.675194 7 GTACTTT 9510 0.0 19.635796 14-15 CGCAGAG 19300 0.0 19.324387 8 AGAGTAC 14495 0.0 19.20113 10-11 AGTACTT 9600 0.0 18.660788 12-13 CATGGGG 6145 0.0 18.377884 4 GCAGAGT 20860 0.0 17.766088 9 CTATCGT 165 0.0014748229 17.254715 4 >>END_MODULE