##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939097_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1062393 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25046569395695 33.0 31.0 34.0 31.0 34.0 2 32.28858906261619 34.0 31.0 34.0 31.0 34.0 3 32.30978460889708 34.0 31.0 34.0 30.0 34.0 4 35.79050596154154 37.0 35.0 37.0 35.0 37.0 5 35.87007632768665 37.0 35.0 37.0 35.0 37.0 6 36.00437879391148 37.0 37.0 37.0 35.0 37.0 7 35.95286678281954 37.0 37.0 37.0 35.0 37.0 8 35.956337249963056 37.0 37.0 37.0 35.0 37.0 9 37.62053966846543 39.0 38.0 39.0 35.0 39.0 10-11 37.55510343159264 39.0 38.0 39.0 35.0 39.0 12-13 37.46339113680154 39.0 38.0 39.0 35.0 39.0 14-15 38.665192635870156 40.0 38.0 41.0 34.5 41.0 16-17 38.644633859598095 40.0 38.0 41.0 34.5 41.0 18-19 38.70533314884416 40.0 38.0 41.0 35.0 41.0 20-21 38.66415770811743 40.0 38.0 41.0 35.0 41.0 22-23 38.55324677402807 40.0 38.0 41.0 34.5 41.0 24-25 38.54441200196161 40.0 38.0 41.0 35.0 41.0 26-27 38.40275679527256 40.0 38.0 41.0 34.5 41.0 28-29 38.24180176262456 40.0 38.0 41.0 34.0 41.0 30-31 37.932557443431946 40.0 37.0 41.0 34.0 41.0 32-33 37.56524468817095 40.0 35.5 41.0 33.5 41.0 34-35 37.192971433358466 40.0 35.0 41.0 33.0 41.0 36-37 36.86930683842985 40.0 35.0 41.0 32.5 41.0 38-39 36.574653635707314 39.0 35.0 41.0 31.5 41.0 40-41 36.34279452142475 39.0 35.0 41.0 31.0 41.0 42-43 36.032172651739984 39.0 35.0 41.0 30.0 41.0 44-45 35.55545546704468 39.0 35.0 41.0 28.5 41.0 46-47 34.89230727235589 39.0 35.0 41.0 24.0 41.0 48-49 34.52775102998608 39.0 35.0 41.0 20.0 41.0 50-51 33.45903634530725 38.0 34.0 40.0 15.0 40.5 52-53 33.59015260830973 38.0 34.0 39.5 13.5 40.5 54-55 34.050129754243486 39.0 35.0 40.5 9.5 41.0 56-57 33.95747289374083 39.0 35.0 41.0 7.5 41.0 58-59 33.787691560467735 38.5 35.0 41.0 3.5 41.0 60-61 33.497025112176004 38.0 34.5 40.5 2.0 41.0 62-63 33.141660854316626 37.5 34.0 40.0 2.0 41.0 64-65 32.77013308634375 37.0 34.0 40.0 2.0 41.0 66-67 32.39069346277695 36.5 33.0 40.0 2.0 41.0 68-69 31.993854439929482 36.0 33.0 39.0 2.0 41.0 70-71 31.584686175454845 35.5 33.0 39.0 2.0 41.0 72-73 31.119735352171936 35.0 32.0 38.5 2.0 40.0 74-75 30.66315525422325 35.0 31.5 37.0 2.0 40.0 76-77 30.198644475255392 35.0 31.0 37.0 2.0 39.0 78-79 29.75741368777844 35.0 31.0 36.0 2.0 39.0 80-81 29.32207102268181 35.0 30.0 36.0 2.0 38.5 82-83 28.865946970659635 35.0 29.5 35.5 2.0 37.0 84-85 28.454275395263334 34.5 29.0 35.0 2.0 37.0 86-87 28.03673169909817 34.0 29.0 35.0 2.0 36.0 88-89 27.584396734541738 34.0 27.5 35.0 2.0 36.0 90-91 27.163478110266162 34.0 26.5 35.0 2.0 36.0 92-93 26.750543348836068 34.0 25.0 35.0 2.0 35.0 94-95 26.301990412210927 34.0 24.5 35.0 2.0 35.0 96-97 25.74703381893518 34.0 21.0 35.0 2.0 35.0 98-99 25.10988400714237 33.5 8.5 35.0 2.0 35.0 100-101 22.816510933336346 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12417821440521948 1101 2 0.17878989710789028 1101 3 0.24934072099075166 1101 4 -0.015789333369056635 1101 5 0.14255974265887517 1101 6 0.01858311372626531 1101 7 0.1344252630566487 1101 8 0.13515911516050494 1101 9 0.2065781665871782 1101 10-11 0.21738685300650928 1101 12-13 0.2103232133332682 1101 14-15 0.2069979422953665 1101 16-17 0.17642568162347771 1101 18-19 0.16801358504346098 1101 20-21 0.19577380907934838 1101 22-23 0.2014562069429502 1101 24-25 0.13620874508136893 1101 26-27 0.16070394060513848 1101 28-29 -0.017546299737276172 1101 30-31 0.02042816678106618 1101 32-33 -0.09353888170932834 1101 34-35 -0.055285453611666924 1101 36-37 0.021048803736007926 1101 38-39 0.005841476560782155 1101 40-41 -0.06416524801655044 1101 42-43 -0.17405848845155703 1101 44-45 -0.1291556858206988 1101 46-47 0.01636959050961906 1101 48-49 0.37169185917458947 1101 50-51 0.4136964756994459 1101 52-53 0.4064986647598303 1101 54-55 0.47895594755282644 1101 56-57 0.2191919993077036 1101 58-59 0.18438541911513084 1101 60-61 0.18042369100301414 1101 62-63 0.21786878669009724 1101 64-65 0.3085647253120598 1101 66-67 -0.11492164611225775 1101 68-69 -0.16953860421847367 1101 70-71 -0.16351256265216918 1101 72-73 -0.27340270450894266 1101 74-75 -0.2962442399876508 1101 76-77 -0.32344549939815437 1101 78-79 -0.26160662433261805 1101 80-81 -0.48620898805695845 1101 82-83 -0.41919450699380434 1101 84-85 -0.6228276195162223 1101 86-87 -0.6898117485305839 1101 88-89 -0.6040492739488563 1101 90-91 -0.8627984999176235 1101 92-93 -1.0143187448380004 1101 94-95 -1.0680663309292022 1101 96-97 -0.9658859991667157 1101 98-99 -0.7824593074099084 1101 100-101 -0.9155081755639749 1102 1 0.15307494611319328 1102 2 0.22224993253580294 1102 3 0.22337828538976368 1102 4 0.2553288092350243 1102 5 0.29018052883387924 1102 6 0.28563110366582123 1102 7 0.2885366581318962 1102 8 0.24913691186922193 1102 9 0.5138949759120237 1102 10-11 0.33047219099145053 1102 12-13 0.4162377806936135 1102 14-15 0.6251216154391201 1102 16-17 0.607253369479281 1102 18-19 0.6394887111370622 1102 20-21 0.6523746231098855 1102 22-23 0.7163169969041476 1102 24-25 0.6719476603383541 1102 26-27 0.6199009981957815 1102 28-29 0.6595932348319735 1102 30-31 0.7326237504626008 1102 32-33 0.6750573888949702 1102 34-35 0.6400751132348503 1102 36-37 0.777279112175151 1102 38-39 0.7893052981772186 1102 40-41 0.7316577747141011 1102 42-43 0.8608893838457092 1102 44-45 0.7647228586602495 1102 46-47 0.6621919371351837 1102 48-49 0.6157252317220241 1102 50-51 0.8570997182994873 1102 52-53 0.6274362873038797 1102 54-55 0.3712591671652845 1102 56-57 0.5425257383041 1102 58-59 0.6350703135751274 1102 60-61 0.5486372861242401 1102 62-63 0.5040302676306894 1102 64-65 0.7116315380174285 1102 66-67 0.5266615215753276 1102 68-69 0.4332834584429506 1102 70-71 0.4653125019158679 1102 72-73 0.5357733938237779 1102 74-75 0.5140339096479281 1102 76-77 0.40639642723091995 1102 78-79 0.25637777116181937 1102 80-81 0.2669788474601944 1102 82-83 0.019922229311688966 1102 84-85 -0.007288020040878251 1102 86-87 0.04182185659700011 1102 88-89 0.1574073268651759 1102 90-91 0.3692002019605951 1102 92-93 0.1845643295470829 1102 94-95 0.38538383336433313 1102 96-97 0.3062665842308476 1102 98-99 0.20876484847288168 1102 100-101 -0.014212758204301679 1103 1 0.3306651061783157 1103 2 0.28044152899634867 1103 3 0.3650995032912405 1103 4 0.29733205581624134 1103 5 0.29550198372615455 1103 6 0.1958238754465711 1103 7 0.2857151671129827 1103 8 0.34309268322127906 1103 9 0.4198260077371856 1103 10-11 0.47292617768720646 1103 12-13 0.484614682761503 1103 14-15 0.5097722466920445 1103 16-17 0.44756877917951954 1103 18-19 0.5378144819592592 1103 20-21 0.49767611200936557 1103 22-23 0.6044713106664688 1103 24-25 0.7669137735448146 1103 26-27 0.6238454257811199 1103 28-29 0.7050544035048745 1103 30-31 0.7354223612726543 1103 32-33 0.7408560526907735 1103 34-35 0.6570751084179562 1103 36-37 0.592901959629188 1103 38-39 0.5635492879076125 1103 40-41 0.5751252342402182 1103 42-43 0.5989061755105709 1103 44-45 0.6914905354279313 1103 46-47 0.8230109041057929 1103 48-49 0.7658470989397372 1103 50-51 0.6799677645223241 1103 52-53 0.5870364616751544 1103 54-55 0.6062822641340162 1103 56-57 0.629637918763919 1103 58-59 0.7395871076484255 1103 60-61 0.8215674701774631 1103 62-63 0.7062648210812696 1103 64-65 0.6917817526096997 1103 66-67 0.544151634916652 1103 68-69 0.48327671480244305 1103 70-71 0.7211987367005293 1103 72-73 0.7873394839091823 1103 74-75 0.702356091889996 1103 76-77 0.7117918213244572 1103 78-79 0.8553238364884272 1103 80-81 0.8275728658510673 1103 82-83 0.9350384607726951 1103 84-85 0.9062169630277666 1103 86-87 0.7148251368964154 1103 88-89 0.720487460581932 1103 90-91 0.6470773280136868 1103 92-93 0.8660513504475524 1103 94-95 0.6230935485418634 1103 96-97 0.658930081386476 1103 98-99 0.8755862576047377 1103 100-101 0.46068524423304424 1104 1 0.0384951237708151 1104 2 0.16873030823992252 1104 3 0.19132611635129138 1104 4 0.14886314569173464 1104 5 0.17958032234326282 1104 6 0.24878565586146806 1104 7 0.12622155061131224 1104 8 0.0685841651064294 1104 9 0.2773946252564201 1104 10-11 0.2679029320751525 1104 12-13 0.1878634153329486 1104 14-15 0.4785404779903999 1104 16-17 0.3736079349205994 1104 18-19 0.4705791495334424 1104 20-21 0.4387124185801028 1104 22-23 0.4161954205268614 1104 24-25 0.5361818533396701 1104 26-27 0.5405676814195388 1104 28-29 0.6573302348520755 1104 30-31 0.5195954461100953 1104 32-33 0.4304819068765653 1104 34-35 0.36427649953669317 1104 36-37 0.3785618139144731 1104 38-39 0.5293405884408173 1104 40-41 0.2716993330205 1104 42-43 0.45976716765500925 1104 44-45 0.579003707187681 1104 46-47 0.5963390516475897 1104 48-49 0.8649089078463348 1104 50-51 0.640413478782186 1104 52-53 0.5904820357377503 1104 54-55 0.6865180668990689 1104 56-57 0.6095908396157697 1104 58-59 0.6015628942229583 1104 60-61 0.7826452027805644 1104 62-63 0.6921998690423052 1104 64-65 0.5309149942871869 1104 66-67 0.5764557996801045 1104 68-69 0.4553718047322093 1104 70-71 0.5445920081360143 1104 72-73 0.42306570577362734 1104 74-75 0.30402964028293056 1104 76-77 0.4741687529941139 1104 78-79 0.4411969263006057 1104 80-81 0.42377985129316187 1104 82-83 0.5957921567797868 1104 84-85 0.557168156287446 1104 86-87 0.3469791549049823 1104 88-89 0.43007842373718574 1104 90-91 0.4475477803081702 1104 92-93 0.5868224806636846 1104 94-95 0.5528807200393011 1104 96-97 0.5037678821887646 1104 98-99 0.3189629541047765 1104 100-101 0.1492991906957748 1105 1 0.17896880534038218 1105 2 0.23796958593661088 1105 3 0.25762342833055385 1105 4 0.09464676449501042 1105 5 0.30272836746867426 1105 6 0.36303035497811464 1105 7 0.18662729535297018 1105 8 0.24266083292938134 1105 9 0.16171548605137076 1105 10-11 0.2409032789587684 1105 12-13 0.24110132294845954 1105 14-15 0.4204576535287785 1105 16-17 0.30794384915526507 1105 18-19 0.27889868388036376 1105 20-21 0.31246587378084456 1105 22-23 0.3178795513652517 1105 24-25 0.3948338723549938 1105 26-27 0.3774053028015274 1105 28-29 0.3395466684085875 1105 30-31 0.43972931264062964 1105 32-33 0.22885798979702088 1105 34-35 0.2991747361025858 1105 36-37 0.19557525510897733 1105 38-39 0.4337065222885812 1105 40-41 0.4494499127960978 1105 42-43 0.5325647294341067 1105 44-45 0.38093152921675255 1105 46-47 0.6183285273297372 1105 48-49 0.698579307329716 1105 50-51 0.5292700064117994 1105 52-53 0.6114046806925586 1105 54-55 0.6226254873721828 1105 56-57 0.5845557694645649 1105 58-59 0.6480033011457991 1105 60-61 0.6979914209856233 1105 62-63 0.6517148428514972 1105 64-65 0.6847164241457904 1105 66-67 0.42698114290944034 1105 68-69 0.607020483629249 1105 70-71 0.5124000091483012 1105 72-73 0.5897290999533986 1105 74-75 0.5605097097469169 1105 76-77 0.46281813220335266 1105 78-79 0.5975465326204343 1105 80-81 0.32664207741328255 1105 82-83 0.5034758469232727 1105 84-85 0.6034274856021646 1105 86-87 0.5847302364618976 1105 88-89 0.5401599741105763 1105 90-91 0.641169376103683 1105 92-93 0.609864606296636 1105 94-95 0.19631927473455946 1105 96-97 0.416696277993875 1105 98-99 0.49207587453753376 1105 100-101 0.3053946338668574 1106 1 -0.023412147040566822 1106 2 0.04260030990371888 1106 3 0.20696584792469253 1106 4 0.16246472075690122 1106 5 0.18121639355396013 1106 6 0.22586679390150977 1106 7 0.21146363369044963 1106 8 0.23363324724275003 1106 9 0.2347597187065915 1106 10-11 0.29072473896329853 1106 12-13 0.4104869525743666 1106 14-15 0.314441149056897 1106 16-17 0.3360198921054476 1106 18-19 0.4261417916497905 1106 20-21 0.561556409484659 1106 22-23 0.4805290681337553 1106 24-25 0.4287072263084468 1106 26-27 0.5121097478606629 1106 28-29 0.4086890058575676 1106 30-31 0.40654015244917474 1106 32-33 0.2765179004432099 1106 34-35 0.3311945850560747 1106 36-37 0.13166757621273462 1106 38-39 0.17259387444824625 1106 40-41 0.4232577601737333 1106 42-43 0.16598146525358715 1106 44-45 0.09837312652104657 1106 46-47 0.20013945095988817 1106 48-49 0.11140799275565882 1106 50-51 -0.07661552681927475 1106 52-53 -0.04584567764474912 1106 54-55 -0.11492805367106484 1106 56-57 0.20891977311918453 1106 58-59 0.22921804368777288 1106 60-61 0.09428210589187103 1106 62-63 -0.0750204737007536 1106 64-65 -0.05152635671941397 1106 66-67 0.009903750431590197 1106 68-69 0.17210271261307497 1106 70-71 -0.03836234825021023 1106 72-73 0.0012019710944635165 1106 74-75 -0.17208787954403348 1106 76-77 -0.15872737034901618 1106 78-79 -0.22930110486793964 1106 80-81 -0.09893796801802068 1106 82-83 -0.16003808003458886 1106 84-85 -0.15206178104271117 1106 86-87 -0.16873683422485186 1106 88-89 -0.08244168052769041 1106 90-91 -0.21388946665539166 1106 92-93 -0.0565694554463505 1106 94-95 -0.007972809992306651 1106 96-97 0.025092267968812365 1106 98-99 -0.3679651142219953 1106 100-101 -0.018911113779850552 1107 1 0.09881543251584901 1107 2 0.2072970175507436 1107 3 0.2362975779618992 1107 4 0.18767271329220847 1107 5 0.3489358739923105 1107 6 0.1909101883084503 1107 7 0.25685695528536456 1107 8 0.27749614980737647 1107 9 0.22386621947374863 1107 10-11 0.3479592873920794 1107 12-13 0.33646220429316287 1107 14-15 0.373826385198214 1107 16-17 0.36124350116146786 1107 18-19 0.3900939816716189 1107 20-21 0.40428242934105896 1107 22-23 0.3941501879163738 1107 24-25 0.3876079598945097 1107 26-27 0.375662838174911 1107 28-29 0.2950212317193035 1107 30-31 0.48228418205810186 1107 32-33 0.2782244933478353 1107 34-35 0.19148333452413624 1107 36-37 0.1773410191480167 1107 38-39 0.4668208371520919 1107 40-41 0.3412563859199125 1107 42-43 0.17015205231763986 1107 44-45 -0.1853175648613643 1107 46-47 -0.14064084801840693 1107 48-49 0.0489609425212123 1107 50-51 -0.06473623471615753 1107 52-53 0.05276533044069964 1107 54-55 0.018789869019350647 1107 56-57 -0.011640157231134651 1107 58-59 -0.0015736358299918152 1107 60-61 0.10201960389100151 1107 62-63 0.08841910402852449 1107 64-65 0.11568168266545342 1107 66-67 0.08518904474444611 1107 68-69 0.19216066942558996 1107 70-71 -0.03442991312709154 1107 72-73 0.028472808413258832 1107 74-75 0.1360880522742356 1107 76-77 0.016264528580862248 1107 78-79 -0.1585553379210829 1107 80-81 -0.08580593814361848 1107 82-83 -0.06556837803684346 1107 84-85 -0.11652785219938622 1107 86-87 -0.20247095905550694 1107 88-89 -0.18451614009847006 1107 90-91 -0.3081454175258145 1107 92-93 -0.4148358654815283 1107 94-95 -0.18216241017033852 1107 96-97 0.06424874160226679 1107 98-99 0.12692047524310368 1107 100-101 0.28438123920036773 1108 1 -0.008593791400031137 1108 2 0.15696890900110816 1108 3 0.15011538229894228 1108 4 0.14367906342339154 1108 5 0.0056526496597300024 1108 6 -0.027001274932139552 1108 7 0.03596955948449221 1108 8 0.17489008841909026 1108 9 0.0777339176987013 1108 10-11 0.08750019355800731 1108 12-13 0.06594228269257485 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4 566.0 5 689.0 6 761.0 7 995.0 8 1272.0 9 1534.0 10 1958.0 11 3005.0 12 5056.0 13 8475.0 14 8044.0 15 9837.0 16 13642.0 17 21101.0 18 18776.0 19 8784.0 20 7711.0 21 7400.0 22 7488.0 23 8183.0 24 8850.0 25 9736.0 26 11022.0 27 12733.0 28 14543.0 29 17175.0 30 20305.0 31 24373.0 32 29902.0 33 38709.0 34 54400.0 35 94539.0 36 122294.0 37 192211.0 38 224294.0 39 48283.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.14487492693172 20.43909906217627 14.980060267758072 18.43596574313394 2 14.547625307314002 22.980067112006942 41.82881632399212 20.643491256686936 3 19.362194300359466 31.13809072877946 30.409169929190284 19.09054504167079 4 11.283452695062193 18.287410478703357 39.314172634753106 31.11496419148134 5 11.06311709952385 41.28379320874787 32.98624332241294 14.666846369315333 6 25.848638739657844 38.78741805637589 19.250098676184795 16.113844527781467 7 24.685551922205484 37.77955519881522 21.536407769276934 15.998485109702361 8 25.552072763656614 33.592046044456005 20.816292305793528 20.039588886093863 9 28.36399330781057 17.610142284387905 22.080362795044163 31.945501612757365 10-11 23.412495990527045 29.219814261901362 26.44773178050744 20.91995796706415 12-13 26.803764853952227 27.71582154343562 27.620767553900038 17.859646048712108 14-15 23.626060721679348 27.107842098197565 29.6367580247437 19.629339155379384 16-17 18.355304989190792 31.16136568088584 30.08058628761439 20.40274304230898 18-19 18.210411278964052 28.6155665154577 35.349782607671614 17.824239597906626 20-21 20.2272614849729 27.07134663265282 34.88605126103271 17.81534062134157 22-23 19.29308124778858 27.55877836916439 34.479886285416256 18.668254097630772 24-25 18.78301630300337 28.029677978105283 35.18167300978632 18.00563270910502 26-27 18.97804543957419 27.462496118500816 35.680483939899545 17.878974502025446 28-29 18.11479584745088 27.881239696017495 35.90222012392814 18.101744332603484 30-31 18.279621565365535 28.681909774783243 35.334197395281954 17.704271264569265 32-33 17.921147459969067 28.649658849628608 34.93735015930401 18.491843531098322 34-35 18.492356446713256 28.238654122721783 35.02365917939333 18.245330251171627 36-37 18.984617610799877 28.002247136815733 34.949881566621315 18.063253685763073 38-39 18.971251410577096 28.244776432690124 34.872113192222834 17.911858964509953 40-41 18.24805167613278 28.573589193271744 35.173667745379625 18.00469138521585 42-43 17.91019865495616 29.15153681471227 34.25691623357465 18.681348296756916 44-45 18.340129634077837 29.214667904879533 33.573599586926136 18.871602874116498 46-47 18.63006987683009 29.43532503814924 32.49237133409088 19.44223375092979 48-49 18.73112411147947 29.58268502272437 32.274801723363296 19.411389142432856 50-51 19.122202650462828 29.99326153518779 31.948693092482472 18.935842721866912 52-53 18.635140566996903 30.41666469628544 31.782706358026154 19.16548837869151 54-55 18.156346130023348 30.164200600101942 31.930132474441763 19.749320795432943 56-57 18.864177618610473 30.705654672708555 30.76285279661009 19.667314912070886 58-59 18.80143259812957 29.92524136575111 31.419799647994495 19.853526388124823 60-61 18.855527928419942 29.884503035179165 31.787389585289034 19.47257945111186 62-63 19.426619850218252 29.61236491300395 31.82013520164182 19.140880035135975 64-65 19.053305311425294 29.913749399395424 31.93561140600113 19.097333883178152 66-67 18.761501843369192 29.977463363185112 31.4655908358893 19.795443957556394 68-69 18.81129436383942 30.39240218326545 31.47774375721649 19.31855969567864 70-71 18.800458796958697 30.29781598458064 31.114379973749244 19.787345244711418 72-73 18.90043823182492 29.88371202124852 30.926132445708156 20.289717301218406 74-75 18.684507877618916 30.448365188922384 30.969204188090423 19.897922745368284 76-77 18.854122187687224 29.793693193989114 31.49095194118679 19.86123267713687 78-79 18.571764903883885 29.850694053763753 31.682861727244106 19.894679315108256 80-81 18.399560518319852 30.66561902100917 31.025014431129826 19.909806029541148 82-83 18.438026177111837 30.467714564416966 30.7459308970018 20.348328361469402 84-85 18.58817566145872 29.92379341144229 30.629024700353536 20.859006226745453 86-87 18.778554412617233 29.910507834701928 30.79469572380087 20.516242028879972 88-89 19.243939937792646 29.25594727177198 31.002614751853947 20.497498038581423 90-91 18.74295541650723 29.461317284493095 31.322009655602344 20.47371764339733 92-93 18.675155223101285 30.03978478756853 31.07498382501504 20.210076164315147 94-95 18.582573185601543 29.82776185958165 30.90046644676538 20.689198508051433 96-97 18.875914197079204 29.292617322057033 30.504467936472203 21.32700054439156 98-99 19.23686469435097 29.07835899424669 30.400934528609298 21.28384178279304 100-101 19.792691721145776 28.64731820922342 31.04370544633463 20.516284623296173 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8589.0 1 5395.0 2 3191.0 3 3757.5 4 2744.0 5 2556.5 6 4380.5 7 8054.0 8 11492.0 9 11391.5 10 8205.5 11 5801.5 12 5320.5 13 5206.5 14 4917.5 15 4739.0 16 4692.5 17 4711.5 18 4686.5 19 4794.0 20 5128.5 21 5538.0 22 6104.5 23 6738.5 24 7419.0 25 8457.0 26 10142.5 27 11878.0 28 12774.0 29 13833.5 30 15694.5 31 17743.0 32 20927.5 33 24731.5 34 28146.0 35 30169.0 36 33302.0 37 36811.5 38 36672.5 39 35452.5 40 34358.0 41 33090.0 42 31670.5 43 31253.5 44 31906.5 45 32084.5 46 32105.5 47 33826.5 48 49651.5 49 49264.5 50 33190.0 51 27452.5 52 21091.5 53 18425.0 54 16951.0 55 15066.0 56 13346.0 57 12064.0 58 11054.5 59 10078.0 60 8974.0 61 8097.5 62 6941.5 63 4734.0 64 3042.0 65 2477.0 66 2037.0 67 1621.0 68 1387.5 69 1124.5 70 932.0 71 840.5 72 752.0 73 735.5 74 639.5 75 500.5 76 406.5 77 311.5 78 240.5 79 180.5 80 126.5 81 91.0 82 72.5 83 58.5 84 37.5 85 21.5 86 16.0 87 14.5 88 11.5 89 10.0 90 8.0 91 6.5 92 4.5 93 4.0 94 4.0 95 1.0 96 2.5 97 2.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32492683969115005 2 0.11125826318509253 3 0.10335158458310625 4 0.11229366157344788 5 0.12998956130170286 6 0.07906678601986271 7 0.0875382273791337 8 0.09262109219469632 9 0.08066694716550278 10-11 0.07949035808782626 12-13 0.08386726945678294 14-15 0.08570274841795833 16-17 0.07511344671886958 18-19 0.08923251565098791 20-21 0.09982181735007667 22-23 0.10725786032099233 24-25 0.11949438672882821 26-27 0.11949438672882821 28-29 0.11422326766083737 30-31 0.10603420768020874 32-33 0.0995394359714343 34-35 0.3196557206231592 36-37 0.4751066695657821 38-39 0.44790393008990087 40-41 0.42324262302180077 42-43 0.45943450305113076 44-45 0.4663999103909759 46-47 0.4736947626725703 48-49 0.48550771701244266 50-51 0.47350650842014214 52-53 0.4770362756531717 54-55 0.4617876812064839 56-57 0.4391971709150945 58-59 0.4193363472839147 60-61 0.38154430610894463 62-63 0.4506336167501104 64-65 0.4824015218473766 66-67 0.4918612980318959 68-69 0.5038154430610895 70-71 0.49703828997367266 72-73 0.48851978505129456 74-75 0.4944027304396772 76-77 0.4717180930220738 78-79 0.4486098835365067 80-81 0.4502571082452539 82-83 0.46522332131329935 84-85 0.46484681280844287 86-87 0.46894134279875715 88-89 0.48216620403184135 90-91 0.4976030527309574 92-93 0.4899316919445064 94-95 0.4943556668765702 96-97 0.4941203490610348 98-99 0.4871549417211898 100-101 0.47350650842014214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1062393.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.08615122795884 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48846854528763 30.928468007080717 2 13.383135268810618 11.264893096547864 3 5.167714531665505 6.524676458477847 4 2.4672414414939388 4.153467856904043 5 1.3903279309021819 2.925677577820217 6 0.8672538877693734 2.1899626964218255 7 0.5783665201275517 1.7038854581893512 8 0.4183380490799428 1.4084990718390196 9 0.31558764998882355 1.1953682606795195 >10 1.6468664974211276 13.117727385359265 >50 0.1590772709684562 4.645500270454879 >100 0.10485197383520367 7.985405465766742 >500 0.007169365698048138 2.0766636692814124 >1k 0.004480853561280086 4.396409382669594 >5k 4.480853561280086E-4 1.400126172591704 >10k+ 6.721280341920129E-4 4.083269169916071 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19394 1.8255014857966874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13244 1.2466196595798353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10667 1.0040540553260422 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8654 0.8145761502570141 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6195 0.5831175468964875 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4704 0.4427740017112312 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3874 0.3646484869535097 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3719 0.35005878239032073 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3701 0.3483644941184665 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3401 0.32012635625422986 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2741 0.25800245295290913 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2712 0.2552727662926996 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2205 0.20755031330213963 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2197 0.20679729629242663 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2175 0.20472649951571595 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2103 0.19794934642829914 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1832 0.172440895224272 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1724 0.1622751655931468 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1651 0.15540388537951588 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1566 0.14740307965131547 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1505 0.141661324952254 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1467 0.13808449415611737 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1176 0.1106935004278078 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1146 0.10786968664138412 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.412712621412227E-5 0.0 12-13 0.0 0.0 0.0 9.412712621412227E-5 0.0 14-15 0.0 0.0 0.0 9.412712621412227E-5 0.0 16-17 0.0 0.0 0.0 1.8825425242824454E-4 0.0 18-19 0.0 0.0 0.0 3.294449417494279E-4 0.0 20-21 0.0 0.0 0.0 5.647627572847336E-4 0.0 22-23 0.0 0.0 0.0 0.002023733213603629 0.0 24-25 0.0 0.0 0.0 0.004753419873813175 0.0 26-27 0.0 0.0 0.0 0.007812551475772148 0.0 28-29 0.0 0.0 0.0 0.015907484330186662 0.0 30-31 0.0 0.0 0.0 0.0382626768060407 0.0 32-33 0.0 0.0 0.0 0.07068947178680582 0.0 34-35 0.0 0.0 0.0 0.11535279317540684 0.0 36-37 0.0 0.0 0.0 0.18448916737967963 0.0 38-39 0.0 0.0 0.0 0.28313439565207976 0.0 40-41 0.0 0.0 0.0 0.4053114054780105 0.0 42-43 0.0 0.0 0.0 0.5167579229155312 0.0 44-45 0.0 0.0 0.0 0.6450531959453799 0.0 46-47 0.0 0.0 0.0 0.7977744582277932 0.0 48-49 0.0 0.0 0.0 0.9842402952579695 0.0 50-51 0.0 0.0 0.0 1.1843545655891936 0.0 52-53 0.0 0.0 0.0 1.3753385046776476 0.0 54-55 0.0 0.0 0.0 1.5731937239797325 0.0 56-57 0.0 0.0 0.0 1.7962279495440954 0.0 58-59 0.0 0.0 0.0 2.0725381285456512 0.0 60-61 0.0 0.0 0.0 2.414596105207772 0.0 62-63 0.0 0.0 0.0 2.73458127077268 0.0 64-65 0.0 0.0 0.0 3.0351291847743727 0.0 66-67 0.0 0.0 0.0 3.3692804828345064 0.0 68-69 0.0 0.0 0.0 3.760049247312435 0.0 70-71 0.0 0.0 0.0 4.208659130848942 0.0 72-73 0.0 0.0 0.0 4.676282693880701 0.0 74-75 0.0 0.0 0.0 5.129787187980343 0.0 76-77 0.0 0.0 0.0 5.599716865604348 0.0 78-79 0.0 0.0 0.0 6.134358942500562 0.0 80-81 0.0 0.0 0.0 6.737760885096193 0.0 82-83 0.0 0.0 0.0 7.361682541206503 0.0 84-85 0.0 0.0 0.0 8.003347160608175 0.0 86-87 0.0 0.0 0.0 8.668025862369198 0.0 88-89 0.0 0.0 0.0 9.338305128139963 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTATG 220 0.0 30.13466 7 CATTCCG 170 3.76167E-9 27.855568 9 GACATAT 280 0.0 27.129995 1 CGACCGT 35 0.008506013 27.06862 18-19 GTACATG 12880 0.0 24.807726 1 GTGTACG 115 1.2857902E-4 24.770866 1 GGGCAAC 115 1.3086418E-4 24.706678 7 ATGGGTA 405 0.0 24.56574 5 CATATAG 410 0.0 24.266157 3 TACATGG 12885 0.0 24.120428 2 GAGTACT 11010 0.0 24.021317 12-13 AGTACTT 11210 0.0 23.782839 12-13 ACATGGG 12900 0.0 23.39458 3 CATGGGG 2585 0.0 22.909472 4 CATGGGT 755 0.0 21.96275 4 GTACTTT 12065 0.0 21.922884 14-15 GTATCAA 22690 0.0 21.740463 1 ATGGGGA 1160 0.0 21.64629 5 ATGGGAT 2960 0.0 21.607656 5 CGGTATA 155 3.9850733E-5 21.395967 2 >>END_MODULE