##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939097_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1062393 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42117747387266 34.0 31.0 34.0 31.0 34.0 2 32.59516393650937 34.0 31.0 34.0 31.0 34.0 3 32.648369294601906 34.0 31.0 34.0 31.0 34.0 4 36.15580110185214 37.0 35.0 37.0 35.0 37.0 5 36.144576442051104 37.0 35.0 37.0 35.0 37.0 6 36.21928796594104 37.0 37.0 37.0 35.0 37.0 7 36.172106743926214 37.0 37.0 37.0 35.0 37.0 8 36.18040687391577 37.0 37.0 37.0 35.0 37.0 9 37.86526360772332 39.0 38.0 39.0 35.0 39.0 10-11 37.82084125177782 39.0 38.0 39.0 35.0 39.0 12-13 37.7520752678152 39.0 38.0 39.0 35.0 39.0 14-15 38.96099513080376 40.0 38.0 41.0 35.5 41.0 16-17 38.92887942597514 40.0 38.0 41.0 35.5 41.0 18-19 39.05826280858402 40.5 38.5 41.0 35.0 41.0 20-21 39.148149037126565 41.0 39.0 41.0 35.0 41.0 22-23 39.06590310741882 41.0 39.0 41.0 35.0 41.0 24-25 38.97822745443541 41.0 38.0 41.0 35.0 41.0 26-27 38.7643353260046 40.0 38.0 41.0 35.0 41.0 28-29 38.558613432129164 40.0 38.0 41.0 34.5 41.0 30-31 38.20953733693652 40.0 37.0 41.0 34.0 41.0 32-33 37.84791221327701 40.0 35.5 41.0 33.5 41.0 34-35 37.66059076066955 40.0 35.0 41.0 33.0 41.0 36-37 37.444147787118325 40.0 35.0 41.0 33.0 41.0 38-39 37.22090224615561 40.0 35.0 41.0 33.0 41.0 40-41 36.93838814826529 40.0 35.0 41.0 33.0 41.0 42-43 36.63111814554501 40.0 35.0 41.0 31.5 41.0 44-45 36.17120500605708 40.0 35.0 41.0 30.5 41.0 46-47 35.535426626493205 39.5 35.0 41.0 27.0 41.0 48-49 34.95790023089384 39.0 35.0 41.0 23.5 41.0 50-51 34.62514201430167 39.0 35.0 41.0 21.5 41.0 52-53 34.34826377809341 39.0 35.0 41.0 20.0 41.0 54-55 34.12046860248515 38.0 35.0 40.0 18.5 41.0 56-57 33.876920781669305 38.0 34.5 40.0 14.5 41.0 58-59 33.609571975718964 38.0 34.0 40.0 10.0 41.0 60-61 33.24702158240877 37.5 34.0 40.0 7.5 41.0 62-63 32.855362845952484 37.0 33.0 40.0 2.0 41.0 64-65 32.4480841835366 36.0 33.0 39.5 2.0 41.0 66-67 32.09507122129005 36.0 33.0 39.0 2.0 41.0 68-69 31.64358434214081 35.5 32.0 39.0 2.0 40.0 70-71 31.232776853763156 35.0 31.5 38.5 2.0 40.0 72-73 30.77733098768535 35.0 31.0 37.5 2.0 40.0 74-75 30.284279922778104 35.0 31.0 37.0 2.0 39.0 76-77 28.3225040074624 33.0 28.0 35.0 2.0 37.5 78-79 29.212402096022846 34.0 29.0 36.0 2.0 38.0 80-81 29.22220684812494 34.0 30.0 36.0 2.0 37.5 82-83 28.905907230186948 34.0 29.5 35.0 2.0 37.0 84-85 28.505738460249646 34.0 29.0 35.0 2.0 36.5 86-87 27.93251696876768 34.0 28.5 35.0 2.0 36.0 88-89 27.359586330105714 34.0 26.5 35.0 2.0 36.0 90-91 26.863621560006514 33.5 25.5 35.0 2.0 35.0 92-93 26.57433878047013 33.0 25.0 35.0 2.0 35.0 94-95 26.2618451928806 33.0 24.5 35.0 2.0 35.0 96-97 25.706656576238736 33.0 22.0 35.0 2.0 35.0 98-99 24.987134704389057 33.0 10.0 35.0 2.0 35.0 100-101 21.6634983475983 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11005102081639961 1101 2 0.16966720751239706 1101 3 0.11744427854152661 1101 4 0.06357258373154195 1101 5 0.046100456936031264 1101 6 0.06264003476720603 1101 7 0.13138113562649778 1101 8 0.07766574100850221 1101 9 0.22265695474054326 1101 10-11 0.199025948475402 1101 12-13 0.21816267727404437 1101 14-15 0.21068634593817848 1101 16-17 0.2664425868902356 1101 18-19 0.1616026305486642 1101 20-21 0.21991865904438868 1101 22-23 0.27258089862155543 1101 24-25 0.19022192979992525 1101 26-27 0.16792722108757374 1101 28-29 0.10431752851972931 1101 30-31 0.23373780031505476 1101 32-33 0.38809015741247777 1101 34-35 0.3489840822686645 1101 36-37 0.5189934858994576 1101 38-39 0.5571051570838748 1101 40-41 0.5763958928222834 1101 42-43 0.5297645944931659 1101 44-45 0.7583555287463639 1101 46-47 0.666469829376183 1101 48-49 0.7009954901221676 1101 50-51 0.7515857070304648 1101 52-53 0.48309873585970564 1101 54-55 0.5381532318928137 1101 56-57 0.43121909703819483 1101 58-59 0.5167611211534862 1101 60-61 0.37499815863092323 1101 62-63 0.3581612133997396 1101 64-65 0.23665614662050416 1101 66-67 0.146840437511635 1101 68-69 0.157450830551376 1101 70-71 0.08908119800985403 1101 72-73 0.07176400259637816 1101 74-75 0.20692003026070793 1101 76-77 0.27317970346364717 1101 78-79 0.14697342644239697 1101 80-81 0.08017211737088559 1101 82-83 0.1424553065973555 1101 84-85 0.18753718153417864 1101 86-87 0.27316783646203646 1101 88-89 0.5598304417729381 1101 90-91 0.39184304394366265 1101 92-93 0.3029396571173564 1101 94-95 0.29038585094536984 1101 96-97 0.3043903696088357 1101 98-99 0.08723341222388825 1101 100-101 -0.35481477411721585 1102 1 0.3052560834913436 1102 2 0.21788702280048255 1102 3 0.2107197372788292 1102 4 0.15690213087634675 1102 5 0.22963594569240087 1102 6 0.15510416739198263 1102 7 0.27606762569450893 1102 8 0.23468438798698799 1102 9 0.29568982697020374 1102 10-11 0.3917294223033778 1102 12-13 0.23041370157326213 1102 14-15 0.2166631149671545 1102 16-17 0.20035331397365042 1102 18-19 0.2627209083737725 1102 20-21 0.3076568170636733 1102 22-23 0.38025739585627605 1102 24-25 0.3997266152082162 1102 26-27 0.28619828514177215 1102 28-29 0.33098852225899833 1102 30-31 0.4378066007693988 1102 32-33 0.37492639123666294 1102 34-35 0.40501967244080106 1102 36-37 0.37321170557953565 1102 38-39 0.5132665028034538 1102 40-41 0.5884508466443066 1102 42-43 0.6928546652137655 1102 44-45 0.6703334065876945 1102 46-47 0.6559293509642146 1102 48-49 0.6465690300732163 1102 50-51 0.6171084038784613 1102 52-53 0.5625478454293003 1102 54-55 0.5864879850468441 1102 56-57 0.5829573632342928 1102 58-59 0.4208556136013897 1102 60-61 0.4890097605943353 1102 62-63 0.6044189717056909 1102 64-65 0.4835697268674224 1102 66-67 0.4421834256257071 1102 68-69 0.429150423536111 1102 70-71 0.38877154187690977 1102 72-73 0.4060709272647465 1102 74-75 0.5425656430147505 1102 76-77 0.6687552717299781 1102 78-79 0.5730210512852025 1102 80-81 0.2395841763813351 1102 82-83 0.473151830064964 1102 84-85 0.4980316847997628 1102 86-87 0.38911986004458043 1102 88-89 0.3788371081691615 1102 90-91 0.6472146989137109 1102 92-93 0.6347179487350019 1102 94-95 0.7862548488224022 1102 96-97 0.5345635877338601 1102 98-99 0.2042942481602239 1102 100-101 0.2599405434493498 1103 1 0.21809693839228572 1103 2 0.2054498229526942 1103 3 0.25609721255616336 1103 4 0.13068261574409945 1103 5 0.24147344322374664 1103 6 0.21122931972600867 1103 7 0.18505858439934997 1103 8 0.23850417864511542 1103 9 0.32159504028600594 1103 10-11 0.3872196127148797 1103 12-13 0.351824519336013 1103 14-15 0.47652809765045134 1103 16-17 0.354977765450748 1103 18-19 0.3355281414099025 1103 20-21 0.3427815503286027 1103 22-23 0.3247728893703439 1103 24-25 0.2223032398234679 1103 26-27 0.40716293883173904 1103 28-29 0.5190993636244841 1103 30-31 0.5551784595575668 1103 32-33 0.5340299446535255 1103 34-35 0.39533472087459387 1103 36-37 0.5113822763323626 1103 38-39 0.4702023370640376 1103 40-41 0.41269230750951635 1103 42-43 0.3964758797434129 1103 44-45 0.6395799974365772 1103 46-47 0.8130236607766932 1103 48-49 0.7064766421982824 1103 50-51 0.8847093055995359 1103 52-53 1.0355034385565673 1103 54-55 0.6406815660644583 1103 56-57 0.8098146340829757 1103 58-59 1.0081815433808288 1103 60-61 0.9720717828194481 1103 62-63 0.9352974994726324 1103 64-65 0.9230100287898395 1103 66-67 1.0194330318452671 1103 68-69 1.054465929574075 1103 70-71 0.9807873123619224 1103 72-73 1.0072165031957319 1103 74-75 1.1437057999489006 1103 76-77 0.9631085566054374 1103 78-79 1.1015159429775174 1103 80-81 1.0410730758673736 1103 82-83 0.782166511933724 1103 84-85 0.8239319608073359 1103 86-87 0.9035837647087703 1103 88-89 0.8578228405526076 1103 90-91 1.1020612088389043 1103 92-93 1.0670991041132964 1103 94-95 1.0592325996256164 1103 96-97 1.1436606383545147 1103 98-99 0.8025219953507765 1103 100-101 0.4981313451236957 1104 1 0.08105410586470896 1104 2 0.09826674792906687 1104 3 0.22071549156682124 1104 4 0.09261566476340022 1104 5 0.1700067536431149 1104 6 0.16332594730326377 1104 7 0.11102651088170745 1104 8 -0.0031145119123721088 1104 9 0.1389994008733879 1104 10-11 0.10877991789268293 1104 12-13 0.0839561510061273 1104 14-15 0.029887857415637598 1104 16-17 0.22160493568189565 1104 18-19 0.2613502175053597 1104 20-21 0.2318312389549888 1104 22-23 0.2646859218373194 1104 24-25 0.2731287077883948 1104 26-27 0.27786770146872186 1104 28-29 0.22287779314370226 1104 30-31 0.30234059544038416 1104 32-33 0.39394517052941325 1104 34-35 0.5196710450830437 1104 36-37 0.6173312761831227 1104 38-39 0.5746436509385902 1104 40-41 0.8556048832047409 1104 42-43 0.743612802729892 1104 44-45 0.9496285237823585 1104 46-47 1.1134042285304204 1104 48-49 0.9155650433614682 1104 50-51 1.0840166858552394 1104 52-53 1.0762872769769487 1104 54-55 1.2073080579217148 1104 56-57 1.1787448836793502 1104 58-59 1.2542486707387397 1104 60-61 1.2141093692555884 1104 62-63 1.1482769402211943 1104 64-65 1.1089619230358707 1104 66-67 1.1169323195526815 1104 68-69 1.0260188857013794 1104 70-71 0.921925940328105 1104 72-73 1.0496244817772045 1104 74-75 0.9211087133386435 1104 76-77 0.8014639295527921 1104 78-79 1.143385744937568 1104 80-81 1.0041446624030108 1104 82-83 0.8618047803617515 1104 84-85 0.967572544751583 1104 86-87 1.0035885133260187 1104 88-89 1.1788333236904904 1104 90-91 1.1173291439140023 1104 92-93 1.0166037781094168 1104 94-95 1.06160464700325 1104 96-97 1.029458089477675 1104 98-99 1.249085918522674 1104 100-101 0.6912197327194036 1105 1 0.02854487199752498 1105 2 0.11286858444871228 1105 3 -0.007002690588521432 1105 4 0.0662405112292177 1105 5 0.08656270116988196 1105 6 0.01980012629083916 1105 7 0.010614613864689204 1105 8 0.0060069998893084176 1105 9 0.01769594752705217 1105 10-11 0.03061656843689775 1105 12-13 0.03410799439799206 1105 14-15 0.11087263598826524 1105 16-17 0.08826800261297763 1105 18-19 0.027257230881112093 1105 20-21 0.16205309174980442 1105 22-23 0.005368060822021903 1105 24-25 0.018852658247304532 1105 26-27 -0.005779463357718839 1105 28-29 -0.06291775932844956 1105 30-31 -0.033213120250323414 1105 32-33 -0.03572254115140083 1105 34-35 0.061991391006728236 1105 36-37 -0.12465167634182706 1105 38-39 -0.14394920151369917 1105 40-41 -0.0017506855862094994 1105 42-43 0.23846954416197264 1105 44-45 0.3367560875031259 1105 46-47 0.07919583220171944 1105 48-49 0.026714515291168084 1105 50-51 0.0625409906153962 1105 52-53 0.15497313350676478 1105 54-55 0.16134107076949533 1105 56-57 0.2096382507847423 1105 58-59 0.33637554723726737 1105 60-61 0.33686198888134555 1105 62-63 0.42001279816908266 1105 64-65 0.29716809843680636 1105 66-67 0.2555134128687371 1105 68-69 0.22631635476001222 1105 70-71 0.37755865385242515 1105 72-73 0.6148669621477403 1105 74-75 0.19702953660908307 1105 76-77 0.11859354403202005 1105 78-79 0.21432017084519472 1105 80-81 0.3251412655616157 1105 82-83 0.36979946584303747 1105 84-85 0.33597112056528644 1105 86-87 0.3831997200664716 1105 88-89 0.5563506842334647 1105 90-91 0.6734675657462894 1105 92-93 0.5232687430828982 1105 94-95 0.16689527656427927 1105 96-97 0.17082788704163576 1105 98-99 0.4956672605477337 1105 100-101 -0.1142063777990252 1106 1 -0.008028585056479187 1106 2 0.004858173806724153 1106 3 0.022986580247426502 1106 4 0.05286468034766756 1106 5 -0.022162288561972332 1106 6 0.040606741491046705 1106 7 0.059947813083468304 1106 8 0.04008886005812684 1106 9 0.016113814938051974 1106 10-11 -0.024539088263374254 1106 12-13 0.05397999882377036 1106 14-15 0.07430441778389252 1106 16-17 -0.007070678538056541 1106 18-19 0.014462912308758291 1106 20-21 0.12014858130982731 1106 22-23 0.09336292255829903 1106 24-25 0.11156667573249024 1106 26-27 0.21278534330777887 1106 28-29 0.013717180864851741 1106 30-31 0.17989175732824236 1106 32-33 0.363116320216065 1106 34-35 0.23545913226622872 1106 36-37 0.30303229110187146 1106 38-39 0.44307856858042527 1106 40-41 0.3960005905975592 1106 42-43 0.3984849673352855 1106 44-45 0.706394648789292 1106 46-47 0.4696933971844004 1106 48-49 0.513382690746532 1106 50-51 0.5134299186210285 1106 52-53 0.5597601847468994 1106 54-55 0.3164256886687582 1106 56-57 0.37631434738074887 1106 58-59 0.21433130619989527 1106 60-61 0.34319261477718754 1106 62-63 0.524996201493444 1106 64-65 0.4248692493072923 1106 66-67 0.4386698826384716 1106 68-69 0.15384734779443576 1106 70-71 0.17323618379768746 1106 72-73 0.19956763523513743 1106 74-75 0.09648029561361682 1106 76-77 0.27339421628120064 1106 78-79 0.31139500676442466 1106 80-81 0.21438674548215175 1106 82-83 0.158106982125382 1106 84-85 0.2722908077430972 1106 86-87 0.41940017102664484 1106 88-89 0.23179766288410164 1106 90-91 0.30025715678073084 1106 92-93 0.4261415186673432 1106 94-95 0.1450304137383185 1106 96-97 0.3149109207127694 1106 98-99 0.16997216997850018 1106 100-101 0.1114612611981336 1107 1 0.02207713878877371 1107 2 0.036962812303784176 1107 3 0.07363583709624777 1107 4 0.01432993944526828 1107 5 0.009906236730934381 1107 6 0.06939872553096649 1107 7 0.09668567807084116 1107 8 0.09861567557626216 1107 9 0.182463921716014 1107 10-11 0.1778700528670214 1107 12-13 0.042150484380442776 1107 14-15 -0.01243705852389354 1107 16-17 0.10105323074557049 1107 18-19 0.11750901227791388 1107 20-21 0.08646277009012948 1107 22-23 0.09731439031604339 1107 24-25 0.04895272393287797 1107 26-27 0.16678492946997636 1107 28-29 0.11363119239764785 1107 30-31 0.10095366121636573 1107 32-33 -0.0757747701684508 1107 34-35 -0.1579869067984987 1107 36-37 0.05263748593748829 1107 38-39 0.05120833405933212 1107 40-41 0.05759693086593387 1107 42-43 0.014999828406814686 1107 44-45 -0.060613694336908 1107 46-47 -0.110090431127567 1107 48-49 -0.04336091331220615 1107 50-51 -0.1295578731638969 1107 52-53 0.0016901524597514594 1107 54-55 -0.198816035551296 1107 56-57 -0.17619961964496866 1107 58-59 -0.1585687597799108 1107 60-61 -0.1452726326080196 1107 62-63 -0.33985501210541713 1107 64-65 -0.2384468603050678 1107 66-67 -0.12556574723436853 1107 68-69 -0.20471528811809492 1107 70-71 -0.32579510390156585 1107 72-73 -0.3636075352777475 1107 74-75 -0.2949853734176493 1107 76-77 -0.21186330014542776 1107 78-79 -0.11564802455841416 1107 80-81 -0.39455751915540915 1107 82-83 -0.3386823588184029 1107 84-85 -0.3629438695219811 1107 86-87 -0.26645008996445796 1107 88-89 -0.2728658206150918 1107 90-91 -0.004645161882184112 1107 92-93 0.03949647437498882 1107 94-95 -0.120308913736217 1107 96-97 0.20241536166085083 1107 98-99 0.07170130923491769 1107 100-101 -0.23456521270860975 1108 1 0.044937795448390716 1108 2 0.015217669398175815 1108 3 -0.03688484047233942 1108 4 0.02898926523243972 1108 5 0.08301571749154846 1108 6 -0.005814558854979168 1108 7 -0.03956372499101235 1108 8 -0.028156470137496115 1108 9 -0.14878466084399378 1108 10-11 -0.07461127380150145 1108 12-13 -0.038998070706576016 1108 14-15 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54-55 -0.19447801715809732 2316 56-57 -0.32764944228402015 2316 58-59 -0.20190840813017985 2316 60-61 -0.1115384948211755 2316 62-63 -0.14082103902104137 2316 64-65 -0.16133929451653017 2316 66-67 -0.07765731213538629 2316 68-69 -0.15913409321448668 2316 70-71 0.004954263885590393 2316 72-73 -0.03742375406179477 2316 74-75 -0.09680371427495871 2316 76-77 0.007229741104151088 2316 78-79 0.0345761300969194 2316 80-81 2.910484451419393E-4 2316 82-83 -0.11998827303089143 2316 84-85 -0.3229956981745197 2316 86-87 -0.33754452222451903 2316 88-89 -0.6327673831550946 2316 90-91 -0.6403710901470028 2316 92-93 -0.720364339238138 2316 94-95 -0.34698139761443514 2316 96-97 -0.37405430207872215 2316 98-99 -0.47100719299667304 2316 100-101 -0.2597797668314321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 7.0 5 61.0 6 166.0 7 293.0 8 543.0 9 923.0 10 1580.0 11 2585.0 12 4129.0 13 6382.0 14 7056.0 15 10140.0 16 14079.0 17 21315.0 18 20082.0 19 10440.0 20 7756.0 21 7456.0 22 7717.0 23 8325.0 24 8997.0 25 10211.0 26 11485.0 27 13355.0 28 15851.0 29 18842.0 30 22425.0 31 26969.0 32 32839.0 33 41853.0 34 58866.0 35 98772.0 36 126866.0 37 207988.0 38 212879.0 39 23148.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.29588754932089 21.033032953624673 15.385189284547288 18.28589021250715 2 14.882722307093516 23.34983381855867 41.08423154143524 20.683212332912586 3 19.368632888206154 30.99690980644639 30.733259725920636 18.901197579426825 4 11.383640517209734 18.956638456766942 38.83873481847113 30.8209862075522 5 10.928065226333382 41.313336966640406 33.075801516011495 14.682796291014718 6 25.823588822592015 39.31266489895924 18.677080891910997 16.18666538653775 7 24.4191179723511 37.93087868613592 21.359986370392125 16.290016971120856 8 25.579705438571228 34.0042714889876 20.43989371164908 19.9761293607921 9 27.986912564371185 17.76009442833302 21.882391920880504 32.37060108641529 10-11 23.406310094287143 29.429975536359898 26.226688240603995 20.937026128748965 12-13 26.796722116956715 27.801199744350725 27.537926172329826 17.86415196636273 14-15 23.43126402506062 27.385578096055664 29.529247428425126 19.653910450458593 16-17 18.233777260278742 31.242513821632578 30.084494686195875 20.4392142318928 18-19 18.048547006616193 28.51727185702466 35.64184816729779 17.792332969061356 20-21 20.045359862122584 26.97674024584123 35.25992735268399 17.717972539352196 22-23 19.166541948224435 27.41612567100875 34.84468553539038 18.57264684537643 24-25 18.69041130962634 27.92711130689664 35.5650530362288 17.817424347248227 26-27 18.809724167532643 27.378094926128405 36.10022364794651 17.711957258392445 28-29 17.917522046926138 27.76138396996215 36.365073941564 17.956020041547713 30-31 18.123517451711287 28.48968334651154 35.79972890545578 17.587070296321397 32-33 17.872293962780255 28.49166927869442 35.36859711989819 18.26743963862714 34-35 18.388795797718117 27.976757540384835 35.41636856572655 18.2180780961705 36-37 18.797070706214768 27.878804141610154 35.438975086126625 17.885150066048457 38-39 18.838089580286294 28.025178774097608 35.343489638375225 17.793242007240874 40-41 18.121756566885622 28.408673160789554 35.55995923717983 17.909611035144987 42-43 17.857759791316887 28.947674366610325 34.65087611040587 18.54368973166692 44-45 18.294700973770787 29.032206445054193 33.86025518536473 18.81283739581029 46-47 18.61646977319246 29.346187533327406 32.73829946351412 19.299043229966024 48-49 18.735601403067527 29.428931937670516 32.50088362380358 19.334583035458376 50-51 19.04028833048442 29.868259550147215 32.120253164556964 18.971198954811406 52-53 18.63369705640806 30.23618860261156 31.92304162538798 19.207072715592403 54-55 18.10601161716992 30.077193656208202 32.01070601933559 19.80608870728629 56-57 18.927929683271632 30.518932259531077 30.85421308310578 19.698924974091508 58-59 18.736262753577215 29.85826691605113 31.428701867277137 19.97676846309452 60-61 18.819228486423416 29.932499504607268 31.657784114202496 19.590487894766817 62-63 19.393224286089463 29.555853845190818 31.7046687871169 19.346253081602818 64-65 18.978671773480368 29.923148352530166 31.83556787809161 19.262611995897856 66-67 18.630439622141555 29.998385662803084 31.372454889159044 19.998719825896323 68-69 18.675137124079523 30.356310813952724 31.33587610214626 19.63267595982149 70-71 18.714881740976146 30.267909459074684 31.06926112524326 19.94794767470591 72-73 18.785515341309665 29.785023056439563 30.786959251425788 20.642502350824977 74-75 18.530713210648038 30.449231122569522 30.68925529441553 20.330800372366912 76-77 18.510465595054885 29.74630618427314 31.552805752952622 20.190422467719348 78-79 18.27651024905143 29.713871823085853 31.64051653299021 20.36910139487251 80-81 18.00730542974819 30.51111181185671 31.087987552248038 20.393595206147065 82-83 18.046479405525965 30.399681094744892 30.66507465046789 20.88876484926126 84-85 18.06661941484931 29.804224990187244 30.548158732220564 21.580996862742882 86-87 18.096463525468543 29.83781715831614 30.78142984554397 21.28428947067135 88-89 18.52827738593545 29.196527383336466 30.932268664700263 21.34292656602782 90-91 18.001529877737347 29.42964097955643 31.222026459608728 21.346802683097497 92-93 17.775154768527276 30.0411900304313 30.983873199465734 21.199782001575688 94-95 17.595795529526267 29.83985210745929 30.771098830658712 21.79325353235573 96-97 17.778213900129238 29.356368123660452 30.475021955152187 22.39039602105812 98-99 17.920133134973767 29.208932204610306 30.327928647033715 22.54300601338221 100-101 18.154354298386558 28.822594779491844 31.036571418889725 21.986479503231873 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7639.0 1 4928.0 2 3051.0 3 3649.0 4 2790.0 5 2542.0 6 4268.0 7 7923.5 8 11308.0 9 11107.0 10 7997.0 11 5518.0 12 4863.5 13 4818.5 14 4761.0 15 4534.5 16 4408.5 17 4459.5 18 4535.0 19 4763.5 20 5095.5 21 5518.0 22 6065.5 23 6836.0 24 7562.0 25 8486.5 26 10116.0 27 11857.5 28 12888.0 29 14021.5 30 15986.0 31 18073.0 32 21233.5 33 24798.5 34 27805.5 35 30123.5 36 33439.5 37 37017.5 38 37154.5 39 35981.5 40 34504.5 41 32999.5 42 32046.5 43 31915.0 44 32459.5 45 32626.0 46 32390.0 47 34144.5 48 49391.5 49 48943.5 50 34225.5 51 28263.0 52 21148.0 53 18232.5 54 16750.5 55 14823.5 56 13199.0 57 12094.0 58 11189.0 59 10188.5 60 8834.0 61 7885.5 62 6764.5 63 4644.0 64 2929.0 65 2334.5 66 1890.5 67 1533.0 68 1318.0 69 1062.5 70 928.0 71 842.0 72 701.5 73 706.5 74 635.0 75 475.0 76 372.0 77 280.0 78 205.0 79 133.0 80 98.5 81 70.0 82 43.0 83 30.5 84 19.0 85 10.5 86 4.5 87 1.0 88 1.0 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33048034013778327 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.471441359271004E-4 16-17 0.001741351834961262 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.647627572847335E-4 26-27 8.471441359271004E-4 28-29 0.0 30-31 0.0031061951650660346 32-33 0.0 34-35 0.010636365262195817 36-37 0.02767337510695195 38-39 0.02150804833992694 40-41 0.014166132495225402 42-43 0.010589301699088755 44-45 0.0026355595339954234 46-47 0.0015530975825330173 48-49 6.118263203917947E-4 50-51 9.412712621412227E-5 52-53 4.7063563107061136E-5 54-55 0.0 56-57 0.0 58-59 0.00414159355342138 60-61 0.009836284689375778 62-63 0.003765085048564891 64-65 0.002023733213603629 66-67 0.0035297672330295853 68-69 0.0032003222912801573 70-71 4.7063563107061136E-5 72-73 0.0 74-75 0.0 76-77 0.21428040282644936 78-79 1.8825425242824454E-4 80-81 0.0030591316019589736 82-83 8.000805728200393E-4 84-85 0.0 86-87 0.10848151296177591 88-89 0.40342886295372804 90-91 0.389545111837145 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0011765890776765284 100-101 5.647627572847335E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1062393.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.10075770215847 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64425654996064 29.85733986913371 2 13.708467131725127 11.268567720980755 3 5.279352190662518 6.509561256383391 4 2.6500432779982663 4.356751466769621 5 1.451823063858029 2.9835513987017097 6 0.9074981493823705 2.2379316931753217 7 0.5984736297363223 1.7218403752846723 8 0.44023457889430534 1.447517980739729 9 0.31772033761975677 1.1752691952201844 >10 1.7105559757052076 13.276909650947358 >50 0.16936353639864601 4.851098048607823 >100 0.10825107948849637 7.977333420997228 >500 0.008238982762812704 2.3066240179432613 >1k 0.0045772126460070575 4.442616930407807 >5k 4.577212646007058E-4 1.399099814162306 >10k+ 6.865818969010586E-4 4.187987160545136 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19610 1.8458329450589377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13986 1.316461987230714 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10927 1.028527108141714 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8796 0.8279422021794195 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6078 0.5721046731294352 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4704 0.4427740017112312 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3865 0.3638013428175826 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3862 0.36351896143894025 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3508 0.33019795875914093 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3206 0.30177156664247595 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3031 0.2852993195550046 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2898 0.27278041176852635 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2261 0.21282143237013046 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2255 0.21225666961284573 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2226 0.20952698295263616 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2112 0.1987964905642262 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1973 0.18571282002046324 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1775 0.16707564903006702 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1772 0.16679326765142466 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1503 0.1414730706998258 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1496 0.1408141808163269 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1460 0.13742560427261852 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1180 0.11107000893266428 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1092 0.10278682182582152 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.412712621412227E-5 0.0 12-13 0.0 0.0 0.0 9.412712621412227E-5 9.412712621412227E-5 14-15 0.0 0.0 0.0 9.412712621412227E-5 9.412712621412227E-5 16-17 0.0 0.0 0.0 1.8825425242824454E-4 9.412712621412227E-5 18-19 0.0 0.0 0.0 3.294449417494279E-4 9.412712621412227E-5 20-21 0.0 0.0 0.0 5.647627572847336E-4 9.412712621412227E-5 22-23 0.0 0.0 0.0 0.00207079677671069 9.412712621412227E-5 24-25 0.0 0.0 0.0 0.0048475470000272965 9.412712621412227E-5 26-27 0.0 0.0 0.0 0.007953742165093331 9.412712621412227E-5 28-29 0.0 0.0 0.0 0.015907484330186662 9.412712621412227E-5 30-31 0.0 0.0 0.0 0.038356803932254824 9.412712621412227E-5 32-33 0.0 0.0 0.0 0.07116010741787643 9.412712621412227E-5 34-35 0.0 0.0 0.0 0.11629406443754807 9.412712621412227E-5 36-37 0.0 0.0 0.0 0.18618345565153385 9.412712621412227E-5 38-39 0.0 0.0 0.0 0.285581700933647 9.412712621412227E-5 40-41 0.0 0.0 0.0 0.40964125328386014 9.412712621412227E-5 42-43 0.0 0.0 0.0 0.5223114233621644 9.412712621412227E-5 44-45 0.0 0.0 0.0 0.6518303490327968 9.412712621412227E-5 46-47 0.0 0.0 0.0 0.8059164546453148 9.412712621412227E-5 48-49 0.0 0.0 0.0 0.9934176900638464 9.412712621412227E-5 50-51 0.0 0.0 0.0 1.1945673587834258 9.412712621412227E-5 52-53 0.0 0.0 0.0 1.3876691582116976 9.412712621412227E-5 54-55 0.0 0.0 0.0 1.5885364455526345 9.412712621412227E-5 56-57 0.0 0.0 0.0 1.813688531456815 9.412712621412227E-5 58-59 0.0 0.0 0.0 2.0925401428661523 9.412712621412227E-5 60-61 0.0 0.0 0.0 2.4372336790622677 9.412712621412227E-5 62-63 0.0 0.0 0.0 2.758207179452425 9.412712621412227E-5 64-65 0.0 0.0 0.0 3.0604023181628643 9.412712621412227E-5 66-67 0.0 0.0 0.0 3.3962479044948526 9.412712621412227E-5 68-69 0.0 0.0 0.0 3.7884285758659932 9.412712621412227E-5 70-71 0.0 0.0 0.0 4.236050124577251 9.412712621412227E-5 72-73 0.0 0.0 0.0 4.704944403812902 9.412712621412227E-5 74-75 0.0 0.0 0.0 5.16169628376693 9.412712621412227E-5 76-77 0.0 0.0 0.0 5.63299080472104 9.412712621412227E-5 78-79 0.0 0.0 0.0 6.166173911160936 9.412712621412227E-5 80-81 0.0 0.0 0.0 6.769858235135208 9.412712621412227E-5 82-83 0.0 0.0 0.0 7.393638700556197 9.412712621412227E-5 84-85 0.0 0.0 0.0 8.033467840996693 9.412712621412227E-5 86-87 0.0 0.0 0.0 8.695981618854793 9.412712621412227E-5 88-89 0.0 0.0 0.0 9.365460804052738 9.412712621412227E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACTCG 70 0.008440901 27.110073 9 GACCGGT 70 0.008440901 27.110073 6 ATGGGTA 435 0.0 26.175243 5 GACATAT 335 0.0 25.585487 1 GATAGTC 120 1.7247723E-4 23.721315 7 GTAATAC 140 1.7960365E-5 23.721313 3 GTAGGGT 285 3.6379788E-12 23.30515 3 GTACATG 13555 0.0 23.150047 1 CCCTATA 205 2.7032002E-8 23.142744 2 GAGTACT 11580 0.0 23.107859 12-13 CATGGGG 2450 0.0 22.849918 4 AGTACTT 11770 0.0 22.734835 12-13 TACATGG 13410 0.0 22.713024 2 CATGGGT 755 0.0 22.62165 4 ACATGGG 13330 0.0 21.78161 3 ATGGGAT 2900 0.0 21.75817 5 CATTCCG 220 5.710899E-8 21.56483 9 GTATCAA 23530 0.0 21.430882 1 GTACTTT 12535 0.0 21.252724 14-15 GGGATCG 115 0.003749254 20.62723 7 >>END_MODULE