FastQCFastQC Report
Fri 27 May 2016
SRR939095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836179
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82710.2916247528805481No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72800.25668337576718536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61950.21842768034034524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40970.14445491627996682No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40490.1427624984177656No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31800.11212268337083098No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28600.10083989762282282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG107650.022.7109181
TACATGG105950.022.1820222
GAGTACT80700.022.06737912-13
GTATCAA179750.021.445131
GTGTAGC17700.020.9200571
ACATGGG107300.020.7547993
GTACTTT87050.020.43223814-15
AGAGTAC137600.019.30959710-11
AGTACTT85050.019.1288512-13
ATCAACG200800.018.83043
CAACGCA201400.018.8213585
TCAACGC202000.018.7889084
GGTATCA140500.018.718671
AACGCAG205000.018.4839826
TATCAAC206950.018.3166032
TACTGCG1851.5558116E-417.921977
GTAGCCC16050.017.7131823
TAGCCCT20650.017.668154
TAGCGCG1650.001488968617.2300974
CATGGGG52300.017.2136254
Produced by FastQC (version 0.11.5)