##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939095_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2836179 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.232045650151136 33.0 31.0 34.0 31.0 34.0 2 32.33045763331581 34.0 31.0 34.0 31.0 34.0 3 32.395638991756165 34.0 31.0 34.0 30.0 34.0 4 35.82542392423045 37.0 35.0 37.0 35.0 37.0 5 35.874084463639285 37.0 35.0 37.0 35.0 37.0 6 35.96110435906901 37.0 37.0 37.0 35.0 37.0 7 35.8940352495382 37.0 37.0 37.0 35.0 37.0 8 35.886092520958655 37.0 37.0 37.0 35.0 37.0 9 37.59950517932754 39.0 38.0 39.0 35.0 39.0 10-11 37.51918725863212 39.0 38.0 39.0 35.0 39.0 12-13 37.43848924909182 39.0 38.0 39.0 35.0 39.0 14-15 38.784966498941 40.0 38.0 41.0 35.0 41.0 16-17 38.72974925066436 40.0 38.0 41.0 35.0 41.0 18-19 38.66766642726006 40.0 38.0 41.0 34.5 41.0 20-21 38.57674374572268 40.0 38.0 41.0 34.0 41.0 22-23 38.50041993118206 40.0 38.0 41.0 34.0 41.0 24-25 38.53977975297046 40.0 38.0 41.0 34.0 41.0 26-27 38.47255039262332 40.0 38.0 41.0 34.0 41.0 28-29 38.360187068587706 40.0 38.0 41.0 34.0 41.0 30-31 38.21535964408452 40.0 38.0 41.0 34.0 41.0 32-33 38.03402041972669 40.0 38.0 41.0 33.5 41.0 34-35 37.74398742110424 40.0 38.0 41.0 33.0 41.0 36-37 37.506386056733376 40.0 37.5 41.0 32.5 41.0 38-39 37.29439467678168 40.0 37.0 41.0 31.5 41.0 40-41 37.16586188671448 40.0 37.0 41.0 31.0 41.0 42-43 36.98762366550207 40.0 37.0 41.0 31.0 41.0 44-45 36.74964485668923 40.0 37.0 41.0 30.5 41.0 46-47 36.42357675590998 40.0 36.0 41.0 30.0 41.0 48-49 36.43271898564935 40.0 36.5 41.0 30.0 41.0 50-51 35.44646776525741 38.5 35.0 40.0 28.5 40.5 52-53 35.62297672326042 38.5 35.5 40.0 28.5 40.5 54-55 36.148704119168784 39.5 36.0 41.0 29.0 41.0 56-57 36.050847460615145 39.0 36.0 41.0 29.0 41.0 58-59 35.88163017919532 39.0 35.0 41.0 28.5 41.0 60-61 35.60590833653306 39.0 35.0 41.0 28.0 41.0 62-63 35.26627021778245 38.5 35.0 40.5 28.0 41.0 64-65 34.90626191083144 38.0 35.0 40.0 26.5 41.0 66-67 34.52294266335094 37.0 34.0 40.0 26.0 41.0 68-69 34.131330568345646 37.0 34.0 39.5 26.0 41.0 70-71 33.72654106105433 36.0 34.0 39.0 26.0 41.0 72-73 33.272499902157094 36.0 33.5 39.0 24.5 40.5 74-75 32.818482190298994 35.0 33.0 37.5 23.5 40.0 76-77 32.38368593801731 35.0 33.0 37.0 22.5 39.0 78-79 31.958488163123697 35.0 32.5 37.0 21.5 39.0 80-81 31.532319892362224 35.0 32.0 36.0 20.0 38.5 82-83 31.113393583409227 35.0 32.0 36.0 18.5 37.0 84-85 30.741987723623932 35.0 31.0 35.0 17.0 37.0 86-87 30.381945215728628 34.5 31.0 35.0 10.0 36.5 88-89 30.010806793224262 34.0 31.0 35.0 4.5 36.0 90-91 29.65733351103721 34.0 30.5 35.0 2.0 36.0 92-93 29.277347445277606 34.0 30.0 35.0 2.0 35.0 94-95 28.85227254697253 34.0 29.5 35.0 2.0 35.0 96-97 28.336594411001563 34.0 29.0 35.0 2.0 35.0 98-99 27.69936188794854 34.0 29.0 35.0 2.0 35.0 100-101 25.17937584334416 31.5 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07620102031989973 1101 2 0.16803018353233767 1101 3 0.18389608114487288 1101 4 0.10603453173438737 1101 5 0.20398897018282725 1101 6 0.2465903769227964 1101 7 0.11358425334861266 1101 8 0.1813330197099532 1101 9 0.3725447437653173 1101 10-11 0.2809640328728591 1101 12-13 0.2529649697885574 1101 14-15 0.38255191818500833 1101 16-17 0.35950805945150677 1101 18-19 0.36261882893989394 1101 20-21 0.33329415186406663 1101 22-23 0.3474639648288331 1101 24-25 0.3330225098993722 1101 26-27 0.2943305747639542 1101 28-29 0.2768954647345012 1101 30-31 0.34509048005087806 1101 32-33 0.2939268720716157 1101 34-35 0.1811279798584451 1101 36-37 0.2236419866389383 1101 38-39 0.18102195062053283 1101 40-41 0.12953979527046755 1101 42-43 0.1619049115124369 1101 44-45 0.14290158604971737 1101 46-47 0.17306951159010708 1101 48-49 0.27308229079846313 1101 50-51 0.10415647183452847 1101 52-53 0.16877972076255787 1101 54-55 0.2777737558251161 1101 56-57 0.3304630539622977 1101 58-59 0.25512738230045784 1101 60-61 0.12471387801173961 1101 62-63 0.14163051139561134 1101 64-65 0.18396333761356942 1101 66-67 0.1996959812975092 1101 68-69 0.16467277809400116 1101 70-71 0.0900262146726547 1101 72-73 0.01505024921720377 1101 74-75 -0.057153915842015124 1101 76-77 -0.02255927490372045 1101 78-79 0.04573694917983673 1101 80-81 -0.030885419427505667 1101 82-83 -0.048000125820184536 1101 84-85 -0.12707710026450414 1101 86-87 -0.16879256733936288 1101 88-89 -0.33839201214416903 1101 90-91 -0.44872948156777426 1101 92-93 -0.4942975490327832 1101 94-95 -0.624742168220731 1101 96-97 -0.8568965416634313 1101 98-99 -1.0169674814412417 1101 100-101 -1.0993641488650354 1102 1 0.26837068467223446 1102 2 0.2651538920849177 1102 3 0.35317386722799426 1102 4 0.2702629391255442 1102 5 0.292857545009781 1102 6 0.33228587349132965 1102 7 0.34025487390508147 1102 8 0.3982772347362058 1102 9 0.3999520622192847 1102 10-11 0.30825518539089103 1102 12-13 0.2763563125671169 1102 14-15 0.40128322604842737 1102 16-17 0.4737394485984012 1102 18-19 0.5098968944094509 1102 20-21 0.46470960356738544 1102 22-23 0.5008022650320001 1102 24-25 0.4540130900019932 1102 26-27 0.46173034488283804 1102 28-29 0.4250965674122824 1102 30-31 0.4175139775931811 1102 32-33 0.410208973939838 1102 34-35 0.3659135916207745 1102 36-37 0.39246466693508353 1102 38-39 0.47946612766003227 1102 40-41 0.45416493138966274 1102 42-43 0.4002359942328795 1102 44-45 0.37221518587848834 1102 46-47 0.3054490098545628 1102 48-49 0.3199716660266887 1102 50-51 0.32012691895901213 1102 52-53 0.32870051225940244 1102 54-55 0.26077186629058957 1102 56-57 0.23939010387852022 1102 58-59 0.23327781146905124 1102 60-61 0.2040352794206015 1102 62-63 0.22858814840729025 1102 64-65 0.22908898389891874 1102 66-67 0.21033749991796213 1102 68-69 0.1990942480423996 1102 70-71 0.13994415512086533 1102 72-73 0.1106171545060235 1102 74-75 0.13520633580183272 1102 76-77 0.10400408413807583 1102 78-79 0.143722997830654 1102 80-81 0.05064117415755476 1102 82-83 -0.137517906478962 1102 84-85 -0.049041529960831554 1102 86-87 -0.04593663352290278 1102 88-89 -0.0673216598640245 1102 90-91 -0.006848639062479123 1102 92-93 -0.01970823482843187 1102 94-95 -0.19129413940634876 1102 96-97 -0.2018753875360666 1102 98-99 -0.5006219049371516 1102 100-101 -0.47730356775760185 1103 1 0.18503388040715407 1103 2 0.2397091856296214 1103 3 0.2552645963158966 1103 4 0.17110893868980526 1103 5 0.20406283406897785 1103 6 0.1374182601680758 1103 7 0.23157332601904557 1103 8 0.27798205602059767 1103 9 0.24047417579244268 1103 10-11 0.2736013227244456 1103 12-13 0.2595921928167684 1103 14-15 0.33879500051689604 1103 16-17 0.3207465709503552 1103 18-19 0.2920089136587052 1103 20-21 0.2910478880513523 1103 22-23 0.3073245996768179 1103 24-25 0.3214040600259622 1103 26-27 0.26088987210989245 1103 28-29 0.1838277763217917 1103 30-31 0.2666605288753985 1103 32-33 0.2784392152603701 1103 34-35 0.10749813070239611 1103 36-37 -0.03623435842727929 1103 38-39 0.1420825907699026 1103 40-41 0.014582132045831031 1103 42-43 -0.02438481982343177 1103 44-45 -0.09217241360375539 1103 46-47 -0.09694008586625813 1103 48-49 -0.0114341254098278 1103 50-51 0.11902404374595221 1103 52-53 0.1455232640568198 1103 54-55 0.1387097163207045 1103 56-57 0.0298062472354772 1103 58-59 0.01907836318699907 1103 60-61 0.1764215076178104 1103 62-63 0.07731009793887011 1103 64-65 -0.03752399012356733 1103 66-67 -0.07752310319114031 1103 68-69 -0.1784831590754692 1103 70-71 -0.0718376023026579 1103 72-73 -0.19459428931615008 1103 74-75 -0.12560270634916293 1103 76-77 -0.09855834457433588 1103 78-79 -0.10916911599882084 1103 80-81 -0.16858653284881697 1103 82-83 -0.2585283641293259 1103 84-85 -0.11766769779586639 1103 86-87 -0.15927366206357263 1103 88-89 -0.03595495645887681 1103 90-91 -0.16647302550854093 1103 92-93 -0.3393618251602355 1103 94-95 -0.29113802757613527 1103 96-97 -0.3633084765158081 1103 98-99 -0.5156769645944586 1103 100-101 -0.8919016659005976 1104 1 0.2096121892334324 1104 2 0.21426915464187601 1104 3 0.24294251430797686 1104 4 0.15872613913121114 1104 5 0.21914896580675958 1104 6 0.2303357291367405 1104 7 0.20271551967253032 1104 8 0.29314727626027803 1104 9 0.36763976843310076 1104 10-11 0.3243021122394296 1104 12-13 0.27063453567974705 1104 14-15 0.3101916629821133 1104 16-17 0.31013041858728485 1104 18-19 0.3020130642481931 1104 20-21 0.35267651335618666 1104 22-23 0.3040056071024111 1104 24-25 0.397447965976653 1104 26-27 0.4649124409791696 1104 28-29 0.3934790956674661 1104 30-31 0.39000114935272023 1104 32-33 0.3467824232394676 1104 34-35 0.36452953444234737 1104 36-37 0.36578814318562536 1104 38-39 0.36128602632460627 1104 40-41 0.2596849502120193 1104 42-43 0.176121813646823 1104 44-45 0.2433098020699731 1104 46-47 0.317779701080525 1104 48-49 0.3640427408919962 1104 50-51 0.29524575870850356 1104 52-53 0.33696149780506346 1104 54-55 0.22134272200760563 1104 56-57 0.14242788472865442 1104 58-59 0.1459290808373055 1104 60-61 0.19407225528438943 1104 62-63 0.18373301840946255 1104 64-65 0.11425984480938922 1104 66-67 0.15541028311695726 1104 68-69 0.2709498635500722 1104 70-71 0.26509521793104085 1104 72-73 0.19573914967610762 1104 74-75 0.18699638780725536 1104 76-77 0.1805006859625493 1104 78-79 0.0738338152577036 1104 80-81 0.15048474083725694 1104 82-83 0.18397522910418473 1104 84-85 0.11376495305144019 1104 86-87 -0.06250825724253772 1104 88-89 0.08231271265973916 1104 90-91 0.03756386647524579 1104 92-93 0.16902093329725432 1104 94-95 0.11225653499594657 1104 96-97 0.09337173068361437 1104 98-99 -0.02702608677435947 1104 100-101 -0.3656184393886406 1105 1 0.1290031338012554 1105 2 0.1829370435933555 1105 3 0.2179915193179056 1105 4 0.19154783504540518 1105 5 0.2739305272457031 1105 6 0.2859262170819221 1105 7 0.27182902831164313 1105 8 0.30129888143597583 1105 9 0.32848718734311433 1105 10-11 0.2412300990932863 1105 12-13 0.34666293825396366 1105 14-15 0.4051182391853487 1105 16-17 0.34619566910185284 1105 18-19 0.4029364005595042 1105 20-21 0.43857827430741736 1105 22-23 0.3139028101990249 1105 24-25 0.2592583228670762 1105 26-27 0.3313011335290881 1105 28-29 0.3199487077774066 1105 30-31 0.33339281329854487 1105 32-33 0.4044362642874262 1105 34-35 0.3154224182810381 1105 36-37 0.3824501455560494 1105 38-39 0.4296661042781551 1105 40-41 0.5028908495899955 1105 42-43 0.5684271749563621 1105 44-45 0.4616511902841296 1105 46-47 0.3477176061417637 1105 48-49 0.41409519927510985 1105 50-51 0.4861943479980795 1105 52-53 0.42323976613126035 1105 54-55 0.48466138074736165 1105 56-57 0.5220568997608481 1105 58-59 0.5290567665450681 1105 60-61 0.5436983342018848 1105 62-63 0.5531666592275641 1105 64-65 0.5091127042023871 1105 66-67 0.4947501685905493 1105 68-69 0.4617209255385646 1105 70-71 0.43374234179442084 1105 72-73 0.5822158598881586 1105 74-75 0.585965862380263 1105 76-77 0.5607570637896799 1105 78-79 0.4446246616361158 1105 80-81 0.2855035594786557 1105 82-83 0.25185725888631794 1105 84-85 0.48418773861699904 1105 86-87 0.2990268015216131 1105 88-89 0.38177936832344983 1105 90-91 0.3384422192371659 1105 92-93 0.45496972590231266 1105 94-95 0.23174601282200413 1105 96-97 0.18289908498714524 1105 98-99 0.00827351652126751 1105 100-101 -0.12083093994177574 1106 1 0.1371098428056996 1106 2 0.2018086900210747 1106 3 0.2006139815593997 1106 4 0.26990709970272064 1106 5 0.30551365120093266 1106 6 0.2059163033242868 1106 7 0.21597213360037415 1106 8 0.1999402908465484 1106 9 0.2790018122491347 1106 10-11 0.2110754241845072 1106 12-13 0.24951142458830589 1106 14-15 0.33533523437557733 1106 16-17 0.38920096076020627 1106 18-19 0.3339772408448667 1106 20-21 0.39250149312811544 1106 22-23 0.41673345853284616 1106 24-25 0.44638909316434905 1106 26-27 0.41670612659114425 1106 28-29 0.34701132169544024 1106 30-31 0.3712456562756685 1106 32-33 0.22357965137710778 1106 34-35 0.12228773771369816 1106 36-37 0.07623874524200858 1106 38-39 0.3094179729002917 1106 40-41 0.2120945956264606 1106 42-43 0.13066115485380436 1106 44-45 0.13341986326088318 1106 46-47 0.09547616133699677 1106 48-49 -0.035900982321940944 1106 50-51 -0.15101827138689572 1106 52-53 -0.167500282631579 1106 54-55 -0.2553902000996473 1106 56-57 -0.1523481620606475 1106 58-59 -0.0842159457665872 1106 60-61 -0.02286812404479832 1106 62-63 -0.09339224111747768 1106 64-65 -0.13009175479049873 1106 66-67 -0.16489465230240086 1106 68-69 -0.2205542733847281 1106 70-71 -0.22704109586162957 1106 72-73 -0.24360437815473546 1106 74-75 -0.2501840010589902 1106 76-77 -0.22362004619498066 1106 78-79 -0.20075581756077554 1106 80-81 -0.17910449484354984 1106 82-83 -0.07423611473328151 1106 84-85 -0.015952246007945092 1106 86-87 -0.132900584571626 1106 88-89 -0.09655211075959258 1106 90-91 0.0013337393955161758 1106 92-93 -0.11483483668061112 1106 94-95 -0.27094751116658244 1106 96-97 -0.11900336735749661 1106 98-99 -0.180729680228783 1106 100-101 -0.0854557614208673 1107 1 0.0027285509628782734 1107 2 0.06141823748430397 1107 3 0.06671156746011064 1107 4 0.02616749594708523 1107 5 0.0863492928634031 1107 6 0.06072129950755567 1107 7 0.042292262832653194 1107 8 0.1183022862891221 1107 9 0.10089892849374138 1107 10-11 0.21184814034078414 1107 12-13 0.18583969420294721 1107 14-15 0.19259245670669856 1107 16-17 0.16658253491439723 1107 18-19 0.1582845870613383 1107 20-21 0.15558690836176936 1107 22-23 0.19452252367236156 1107 24-25 0.18548296263942632 1107 26-27 0.1938511604860409 1107 28-29 0.07926311755439741 1107 30-31 0.16934927614381223 1107 32-33 0.16699725299235268 1107 34-35 0.3466463421841368 1107 36-37 0.31431169644204715 1107 38-39 0.4613763159293711 1107 40-41 0.30345960212070366 1107 42-43 0.2749925069742858 1107 44-45 0.3503117094235648 1107 46-47 0.358728010853973 1107 48-49 0.38226208741382806 1107 50-51 0.3071567055792457 1107 52-53 0.37156714880141095 1107 54-55 0.46879145419107004 1107 56-57 0.47996574499662614 1107 58-59 0.43919392262023393 1107 60-61 0.4998021262955561 1107 62-63 0.344967551379618 1107 64-65 0.3038374748445918 1107 66-67 0.2167268234229951 1107 68-69 0.3240483618989103 1107 70-71 0.37740049520967744 1107 72-73 0.3973006792094722 1107 74-75 0.38619625483320874 1107 76-77 0.4393712248020947 1107 78-79 0.39752312117495947 1107 80-81 0.4065665582102227 1107 82-83 0.47630813683284856 1107 84-85 0.49967498272170374 1107 86-87 0.3845755544550009 1107 88-89 0.4022521104830048 1107 90-91 0.49158482934121395 1107 92-93 0.49788930358419137 1107 94-95 0.4417201128960535 1107 96-97 0.33079333879411266 1107 98-99 0.3164381731612842 1107 100-101 0.38331802573900475 1108 1 -5.737127270677433E-4 1108 2 0.016167282576908804 1108 3 0.07022150436380059 1108 4 -0.01608310020483117 1108 5 0.06740975536202853 1108 6 0.029615486370879296 1108 7 0.16515459158662082 1108 8 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2316 92-93 -1.2056909489083232 2316 94-95 -0.9600397955477895 2316 96-97 -1.062960743537193 2316 98-99 -1.137622371893034 2316 100-101 -0.8582489738296246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7957.0 3 1662.0 4 1766.0 5 1895.0 6 2274.0 7 2589.0 8 3241.0 9 4057.0 10 4995.0 11 6471.0 12 8960.0 13 14481.0 14 12537.0 15 12828.0 16 15532.0 17 21107.0 18 19578.0 19 12668.0 20 12672.0 21 12928.0 22 14213.0 23 15671.0 24 17741.0 25 20352.0 26 23643.0 27 27858.0 28 33303.0 29 40092.0 30 48746.0 31 60748.0 32 77137.0 33 101885.0 34 142887.0 35 214338.0 36 357820.0 37 631553.0 38 697831.0 39 130108.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89897195035108 19.23179104071057 14.672763529477939 23.19647347946041 2 14.969336544703552 20.521375815388808 40.7831897971337 23.726097842773942 3 19.30306034888987 24.49758086975736 30.00159192783166 26.19776685352111 4 12.822830879367983 16.822117811517103 37.61930648856444 32.73574482055047 5 13.914673082008505 37.32102047149797 34.342215037051915 14.42209140944161 6 31.603879951174186 35.916191530154244 17.999419143628558 14.480509375043008 7 28.094954584804853 32.72272714920613 21.262541366348536 17.919776899640482 8 25.518193827732606 35.09353412266959 20.38543759668322 19.002834452914588 9 25.983663715040308 17.594113968631735 21.16041545799614 35.261806858331816 10-11 24.672947227969484 26.631664347568933 27.683639544809875 21.01174887965171 12-13 25.825237647550402 24.09234093071448 27.865181069505734 22.217240352229382 14-15 22.692144471079402 25.46990509347283 26.623817418087203 25.214133017360563 16-17 22.156511324764157 28.601795843010887 27.101113738841264 22.140579093383693 18-19 21.910075580218596 27.51689221679564 29.209334318458986 21.36369788452678 20-21 22.813212862164423 26.546435508287598 29.049304163582708 21.59104746596527 22-23 22.386790529718397 26.58526792135808 28.88727103293631 22.140670515987214 24-25 22.34494829918356 26.659595413464437 29.023081481277508 21.972374806074498 26-27 22.167679155440325 26.844032247131988 29.039364207886397 21.94892438954129 28-29 21.851217864220796 27.336425503733835 28.959250141029237 21.85310649101614 30-31 22.228562130077258 26.75674473636651 28.80430945872679 22.21038367482944 32-33 21.84477807488896 26.871235715838726 28.884695370054015 22.3992908392183 34-35 22.683246955309517 26.868656176319057 28.567057473943773 21.88103939442765 36-37 22.435730173989583 26.65125894938074 28.67867221542887 22.234338661200805 38-39 22.074657286674935 27.164773885445637 28.819300860743642 21.94126796713579 40-41 22.408649488586576 26.845278471413277 28.786360089514833 21.959711950485314 42-43 22.104112096795475 26.966645170808828 28.348301617644477 22.58094111475122 44-45 22.41983408420842 26.899595882482664 28.27270980146609 22.40786023184283 46-47 22.54167068186585 27.159757926003188 27.978734560378204 22.319836831752763 48-49 22.55045147318727 27.25178541311462 27.858910908629653 22.33885220506846 50-51 22.15644236820937 27.401230873116404 28.138273854831514 22.30405290384271 52-53 22.608635576136134 27.43598782643817 27.60018565036085 22.355190947064845 54-55 22.113232919114942 27.16230113142232 27.958554780586116 22.765911168876624 56-57 22.461530179436107 27.48447677927347 27.706292730471837 22.34770031081858 58-59 22.670955583577047 27.110349313727262 27.777270227615002 22.441424875080692 60-61 22.449488142856662 27.08863429898588 28.28575509259939 22.176122465558066 62-63 22.311830813501256 27.180589854647412 28.18885918268747 22.318720149163855 64-65 22.12064667031963 27.23212142438674 27.960960407147834 22.686271498145793 66-67 22.167405470336234 27.34870470176781 27.652978424098112 22.830911403797842 68-69 22.15925551785074 27.149787181713915 27.91862989711967 22.772327403315682 70-71 22.271659124448288 27.071387402602443 27.884816585096367 22.772136887852902 72-73 22.349775743441143 26.90155672107076 28.055065987200123 22.693601548287976 74-75 22.15174465639293 27.433675984233975 27.67036839253626 22.74421096683684 76-77 22.239843495226047 27.061073387652044 27.756498609556974 22.942584507564934 78-79 22.162732423595152 27.241016744257053 27.74024343787595 22.85600739427185 80-81 22.286573487011285 27.24183733467459 27.72911781582732 22.742471362486803 82-83 22.37798592192941 27.13138942125456 27.809680356238243 22.68094430057779 84-85 22.23271007278832 27.182471713800588 27.698600311813305 22.886217901597785 86-87 22.223269267728455 27.203488078589817 27.777961797993626 22.795280855688098 88-89 22.20039296211269 27.129886715588224 27.570361423457513 23.099358898841572 90-91 22.23296128533297 27.05420017948311 27.716303285710364 22.996535249473553 92-93 22.519764778583458 27.03497848480897 27.754472410098252 22.690784326509323 94-95 22.274323873003553 26.83043074221819 27.82823464169189 23.067010743086357 96-97 22.240329430716095 26.7262751718103 27.991866284466372 23.04152911300723 98-99 22.175389674122943 26.88361685617402 27.544264641790356 23.39672882791268 100-101 22.34234697040823 26.292038298362314 28.049883528338487 23.315731202890976 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2551.0 1 1439.5 2 352.0 3 379.0 4 350.5 5 362.0 6 567.0 7 1082.0 8 1775.5 9 2193.5 10 2261.5 11 2336.5 12 2601.0 13 2926.5 14 3227.0 15 3485.5 16 3750.5 17 3926.0 18 3997.0 19 4240.5 20 4636.5 21 5293.5 22 6260.5 23 7515.5 24 9025.0 25 11363.0 26 14857.5 27 18801.5 28 22309.5 29 26250.5 30 31608.0 31 37372.5 32 43603.0 33 49213.5 34 54452.5 35 59862.5 36 65826.5 37 72991.5 38 77433.0 39 79502.5 40 82015.0 41 87122.5 42 93525.5 43 99076.5 44 104226.5 45 108651.0 46 110709.0 47 116998.5 48 131898.0 49 132818.0 50 120593.5 51 111582.5 52 102524.0 53 97061.0 54 92977.5 55 84988.5 56 75204.0 57 64976.5 58 55991.5 59 47784.5 60 39503.5 61 31930.0 62 25432.5 63 19351.5 64 14243.5 65 10739.0 66 7973.0 67 5962.5 68 4399.0 69 3156.0 70 2495.5 71 2029.0 72 1677.5 73 1466.5 74 1200.5 75 964.0 76 706.5 77 429.0 78 281.5 79 203.0 80 139.0 81 103.0 82 83.5 83 63.0 84 41.5 85 30.5 86 24.0 87 23.0 88 20.0 89 12.5 90 8.0 91 6.0 92 4.5 93 8.5 94 10.0 95 8.5 96 8.5 97 5.0 98 3.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33037407018386356 2 0.11734097177928474 3 0.11057130033047984 4 0.11808140459399775 5 0.1340535981685218 6 0.08574917168486193 7 0.09364712170846762 8 0.099711619048022 9 0.08768840048530083 10-11 0.0868069328487377 12-13 0.09065013174415297 14-15 0.09172552226075999 16-17 0.08113028126927109 18-19 0.09518087539608748 20-21 0.10471835522370063 22-23 0.11323333259290053 24-25 0.12634957102496 26-27 0.12534469791927802 28-29 0.12104313585284991 30-31 0.11124121573426783 32-33 0.10475361392916313 34-35 0.3232341823277022 36-37 0.47837248636281415 38-39 0.45358561642265877 40-41 0.43331186078170664 42-43 0.46381064100679115 44-45 0.47110919303753396 46-47 0.47849589183193303 48-49 0.4887914338269904 50-51 0.47770257095902624 52-53 0.48195124496726055 54-55 0.4679535388986379 56-57 0.44662202209381 58-59 0.42860482360245955 60-61 0.3887448570770745 62-63 0.45748170337626787 64-65 0.48704612790659546 66-67 0.4953495530430202 68-69 0.5079192815404105 70-71 0.4998097792840297 72-73 0.49238782178416807 74-75 0.49933378676028556 76-77 0.47660955108968794 78-79 0.45527803428486 80-81 0.45691756408886747 82-83 0.47066845921925243 84-85 0.4702100960482396 86-87 0.47322471536528543 88-89 0.48549474486624433 90-91 0.5001976250441175 92-93 0.4931635133043436 94-95 0.4982583962436785 96-97 0.49709485896341526 98-99 0.49182368249676767 100-101 0.4796594291121964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2836179.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.72242101722235 #Duplication Level Percentage of deduplicated Percentage of total 1 71.97473890359541 36.50733009272819 2 14.894426277693896 15.109627209343396 3 5.598521902843255 8.519117550904692 4 2.6246212031062437 5.325085666987342 5 1.4226940451042818 3.608124316723725 6 0.8560289063142293 2.605191515338964 7 0.5574424230315687 1.9792380491707455 8 0.3688157717162968 1.496578308062862 9 0.2799350290533288 1.2779084161000183 >10 1.2677399986982716 11.483946993878808 >50 0.09484518215908873 3.2980880524894314 >100 0.054480116754680764 5.220091379486179 >500 0.0041085872662567245 1.4740413868149447 >1k 0.0013927414461552534 1.327527352656411 >5k 2.08911216923288E-4 0.7681037093142379 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8271 0.2916247528805481 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7280 0.25668337576718536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6195 0.21842768034034524 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4097 0.14445491627996682 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4049 0.1427624984177656 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3180 0.11212268337083098 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2860 0.10083989762282282 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.762935273126273E-5 0.0 0.0 0.0 0.0 12-13 3.525870546252546E-5 0.0 0.0 0.0 0.0 14-15 3.525870546252546E-5 0.0 0.0 0.0 0.0 16-17 3.525870546252546E-5 0.0 0.0 0.0 0.0 18-19 3.525870546252546E-5 0.0 0.0 0.0 0.0 20-21 3.525870546252546E-5 0.0 0.0 1.762935273126273E-5 0.0 22-23 3.525870546252546E-5 1.762935273126273E-5 0.0 8.814676365631366E-5 0.0 24-25 3.525870546252546E-5 3.525870546252546E-5 0.0 3.1732834916272913E-4 0.0 26-27 3.525870546252546E-5 3.525870546252546E-5 0.0 7.933208729068229E-4 0.0 28-29 3.525870546252546E-5 3.525870546252546E-5 0.0 0.0026444029096894094 0.0 30-31 3.525870546252546E-5 8.814676365631366E-5 0.0 0.008902823129287678 0.0 32-33 3.525870546252546E-5 1.0577611638757639E-4 0.0 0.021331516804827904 0.0 34-35 3.525870546252546E-5 1.234054691188391E-4 0.0 0.0422399291441055 0.0 36-37 3.525870546252546E-5 1.4103482185010184E-4 0.0 0.07566518192257965 0.0 38-39 3.525870546252546E-5 1.4103482185010184E-4 0.0 0.12527418050835298 0.0 40-41 3.525870546252546E-5 1.4103482185010184E-4 0.0 0.1803130197353552 0.0 42-43 3.525870546252546E-5 1.4103482185010184E-4 0.0 0.23961816232332303 0.0 44-45 3.525870546252546E-5 1.4103482185010184E-4 0.0 0.31505416266039626 0.0 46-47 3.525870546252546E-5 1.5866417458136457E-4 0.0 0.404346834244242 0.0 48-49 3.525870546252546E-5 1.762935273126273E-4 0.0 0.49839943106552864 0.0 50-51 3.525870546252546E-5 1.762935273126273E-4 0.0 0.6005262714377336 0.0 52-53 3.525870546252546E-5 3.1732834916272913E-4 0.0 0.70616135300346 0.0 54-55 3.525870546252546E-5 3.3495770189399186E-4 0.0 0.8255297003468398 0.0 56-57 3.525870546252546E-5 3.525870546252546E-4 0.0 0.9723469498927959 0.0 58-59 3.525870546252546E-5 3.525870546252546E-4 0.0 1.1327564303945556 0.0 60-61 3.525870546252546E-5 3.525870546252546E-4 0.0 1.3060529677428683 0.0 62-63 3.525870546252546E-5 3.525870546252546E-4 0.0 1.4848322337906035 0.0 64-65 3.525870546252546E-5 3.525870546252546E-4 0.0 1.6791077008891189 0.0 66-67 3.525870546252546E-5 3.7021640735651737E-4 0.0 1.9065792391806018 0.0 68-69 3.525870546252546E-5 3.878457600877801E-4 0.0 2.1635975726496812 0.0 70-71 3.525870546252546E-5 3.878457600877801E-4 0.0 2.445385146706185 0.0 72-73 3.525870546252546E-5 3.878457600877801E-4 0.0 2.734612307615281 0.0 74-75 3.525870546252546E-5 3.878457600877801E-4 0.0 3.043073092354185 0.0 76-77 3.525870546252546E-5 4.2310446555030555E-4 0.0 3.38030498075051 0.0 78-79 3.525870546252546E-5 4.2310446555030555E-4 0.0 3.7636376265390865 0.0 80-81 3.525870546252546E-5 4.7599252374409373E-4 0.0 4.1804307838116 0.0 82-83 3.525870546252546E-5 5.288805819378819E-4 0.0 4.6092119009413715 0.0 84-85 3.525870546252546E-5 5.288805819378819E-4 0.0 5.0616339800837675 0.0 86-87 3.525870546252546E-5 5.288805819378819E-4 0.0 5.541117115668651 0.0 88-89 3.525870546252546E-5 5.288805819378819E-4 0.0 6.062928327161297 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10765 0.0 22.710918 1 TACATGG 10595 0.0 22.182022 2 GAGTACT 8070 0.0 22.067379 12-13 GTATCAA 17975 0.0 21.44513 1 GTGTAGC 1770 0.0 20.920057 1 ACATGGG 10730 0.0 20.754799 3 GTACTTT 8705 0.0 20.432238 14-15 AGAGTAC 13760 0.0 19.309597 10-11 AGTACTT 8505 0.0 19.12885 12-13 ATCAACG 20080 0.0 18.8304 3 CAACGCA 20140 0.0 18.821358 5 TCAACGC 20200 0.0 18.788908 4 GGTATCA 14050 0.0 18.71867 1 AACGCAG 20500 0.0 18.483982 6 TATCAAC 20695 0.0 18.316603 2 TACTGCG 185 1.5558116E-4 17.92197 7 GTAGCCC 1605 0.0 17.713182 3 TAGCCCT 2065 0.0 17.66815 4 TAGCGCG 165 0.0014889686 17.230097 4 CATGGGG 5230 0.0 17.213625 4 >>END_MODULE