FastQCFastQC Report
Fri 27 May 2016
SRR939095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836179
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85980.30315434956679393No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75400.265850639187442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69600.24540059001917722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44700.1576064134174888No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38280.13497032451054747No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31450.11088862867964257No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG107700.022.1002251
TACATGG108850.021.2734582
GTATCAA184750.021.2317221
GAGTACT92150.021.06227912-13
ACATGGG109000.020.5476533
GTACTTT95800.020.18549714-15
AGTACTT95200.019.73947312-13
GGTATCA143650.019.7175881
AGAGTAC152150.019.46177110-11
ATCAACG211100.018.3645613
CAACGCA212350.018.301155
TCAACGC212100.018.3003484
TATCAAC215400.018.1301272
AACGCAG216400.017.9147826
CATGGGG52400.017.1150134
ATAGCGT2504.609934E-617.0824156
TACTTTT112750.016.62485714-15
ACTTTTT117100.016.57418816-17
GGACCGT3151.3576027E-716.5702426
GTGTAGG11850.016.4705451
Produced by FastQC (version 0.11.5)