##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939095_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2836179 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48021933735494 34.0 31.0 34.0 31.0 34.0 2 32.68991484670044 34.0 31.0 34.0 31.0 34.0 3 32.7843584625653 34.0 31.0 34.0 31.0 34.0 4 36.247356742998235 37.0 35.0 37.0 35.0 37.0 5 36.20604693850424 37.0 37.0 37.0 35.0 37.0 6 36.24385943200341 37.0 37.0 37.0 35.0 37.0 7 36.198047443408896 37.0 37.0 37.0 35.0 37.0 8 36.186829886266 37.0 37.0 37.0 35.0 37.0 9 37.93156426304546 39.0 38.0 39.0 35.0 39.0 10-11 37.866105418593115 39.0 38.0 39.0 35.0 39.0 12-13 37.8036901055963 39.0 38.0 39.0 35.0 39.0 14-15 39.166675481343034 40.0 39.0 41.0 36.0 41.0 16-17 39.111332888368466 40.0 38.0 41.0 36.0 41.0 18-19 39.138158240364945 40.5 38.5 41.0 36.0 41.0 20-21 39.21142512514196 41.0 39.0 41.0 36.0 41.0 22-23 39.17214463544085 41.0 39.0 41.0 36.0 41.0 24-25 39.11592445328733 41.0 39.0 41.0 35.5 41.0 26-27 38.98703731323023 40.0 38.5 41.0 35.0 41.0 28-29 38.83786337181115 40.0 38.0 41.0 35.0 41.0 30-31 38.65901288317839 40.0 38.0 41.0 35.0 41.0 32-33 38.524704364569374 40.0 38.0 41.0 34.0 41.0 34-35 38.472974907437084 40.0 38.0 41.0 34.0 41.0 36-37 38.368822101849005 40.0 38.0 41.0 34.0 41.0 38-39 38.244833277448286 40.0 38.0 41.0 34.0 41.0 40-41 38.08985099318484 40.0 38.0 41.0 33.5 41.0 42-43 37.93531861000311 40.0 38.0 41.0 33.0 41.0 44-45 37.72638997750142 40.0 38.0 41.0 33.0 41.0 46-47 37.45147555919425 40.0 37.0 41.0 32.5 41.0 48-49 37.15756392667741 40.0 37.0 41.0 31.5 41.0 50-51 36.91750785123224 40.0 37.0 41.0 31.0 41.0 52-53 36.71000278896361 40.0 36.0 41.0 31.0 41.0 54-55 36.47733164937756 39.0 36.0 41.0 30.5 41.0 56-57 36.2107173066298 39.0 35.0 41.0 30.0 41.0 58-59 35.9201746434199 39.0 35.0 40.0 29.0 41.0 60-61 35.56902914096748 38.0 35.0 40.0 28.5 41.0 62-63 35.193302503121274 38.0 34.5 40.0 28.0 41.0 64-65 34.78819337566493 37.5 34.0 40.0 28.0 41.0 66-67 34.431682379708754 37.0 34.0 39.5 27.0 41.0 68-69 33.96342614482373 36.0 33.5 39.0 26.0 40.5 70-71 33.51311482808384 36.0 33.0 39.0 26.0 40.0 72-73 33.03375668460982 35.0 33.0 38.0 25.5 40.0 74-75 32.53374540182408 35.0 32.0 37.0 24.5 39.0 76-77 30.516342057394823 33.5 29.5 35.5 20.5 37.5 78-79 31.501896036886247 34.5 31.0 36.0 22.0 38.5 80-81 31.525664282825588 35.0 32.0 36.0 22.0 37.5 82-83 31.22013085210771 35.0 31.5 35.5 21.0 37.0 84-85 30.8366088670708 34.0 31.0 35.0 19.5 36.5 86-87 30.291136243516362 34.0 31.0 35.0 16.5 36.0 88-89 29.749271290704854 34.0 30.0 35.0 8.5 36.0 90-91 29.300002221298442 34.0 29.5 35.0 4.5 35.0 92-93 29.015586992217344 34.0 29.0 35.0 2.0 35.0 94-95 28.680140252078587 34.0 29.0 35.0 2.0 35.0 96-97 28.117071947856605 34.0 29.0 35.0 2.0 35.0 98-99 27.346253533363022 33.5 28.0 35.0 2.0 35.0 100-101 23.669324644177962 29.5 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14221596087302402 1101 2 0.14424831428659246 1101 3 0.20152447803411633 1101 4 0.1512733132856141 1101 5 0.16616778824373313 1101 6 0.13290608400036064 1101 7 0.17193229980616564 1101 8 0.17372828821777375 1101 9 0.21172080795966508 1101 10-11 0.23714914597395165 1101 12-13 0.17415379558426025 1101 14-15 0.2518226562082546 1101 16-17 0.2855556957062433 1101 18-19 0.26363106042271767 1101 20-21 0.3607148308650281 1101 22-23 0.29090042741801625 1101 24-25 0.21604343411525662 1101 26-27 0.23763774155664663 1101 28-29 0.18334504937686802 1101 30-31 0.29008247080357563 1101 32-33 0.30814926728589853 1101 34-35 0.45293859273225934 1101 36-37 0.40609669639760426 1101 38-39 0.45094947980673084 1101 40-41 0.43681975435278986 1101 42-43 0.39078883295623257 1101 44-45 0.4995173582916905 1101 46-47 0.4393125725965561 1101 48-49 0.31357875886470055 1101 50-51 0.49044099976017463 1101 52-53 0.4312484683275102 1101 54-55 0.34196356617684387 1101 56-57 0.3037756351934249 1101 58-59 0.28483690048360444 1101 60-61 0.2870421847385174 1101 62-63 0.37153533384503135 1101 64-65 0.2908260536876881 1101 66-67 0.3404753241299616 1101 68-69 0.3541090442017705 1101 70-71 0.28660692904170304 1101 72-73 0.3538064621302297 1101 74-75 0.35022540682766845 1101 76-77 0.16542839009849786 1101 78-79 0.3794773041851265 1101 80-81 0.4391449822230449 1101 82-83 0.42153938247000156 1101 84-85 0.4380349306397875 1101 86-87 0.4133986110685015 1101 88-89 0.5879245601495313 1101 90-91 0.621944036200567 1101 92-93 0.5707782137928028 1101 94-95 0.5680109345829045 1101 96-97 0.4385686479323212 1101 98-99 0.4400019777901889 1101 100-101 -0.1157761025993409 1102 1 0.17594100704938853 1102 2 0.15682789244576156 1102 3 0.20386707605014465 1102 4 0.07317467680574907 1102 5 0.13458045670272156 1102 6 0.0718921726802293 1102 7 0.13509560770879858 1102 8 0.10883041539415217 1102 9 0.2725961697528376 1102 10-11 0.24024943671556542 1102 12-13 0.15723082046191195 1102 14-15 0.21038850522031538 1102 16-17 0.3157549995275488 1102 18-19 0.2995898909536052 1102 20-21 0.29520533050109066 1102 22-23 0.29433353111531346 1102 24-25 0.2792966848417038 1102 26-27 0.2609481276245731 1102 28-29 0.18046687901487246 1102 30-31 0.309342733965984 1102 32-33 0.3572551081777746 1102 34-35 0.40116379453177586 1102 36-37 0.3872395829495616 1102 38-39 0.39819821787052234 1102 40-41 0.4267825109982226 1102 42-43 0.46384013304258076 1102 44-45 0.42115338639698763 1102 46-47 0.4674512789720282 1102 48-49 0.5109397008054231 1102 50-51 0.6064821104110933 1102 52-53 0.6468144586666185 1102 54-55 0.558488211409184 1102 56-57 0.5784394524452878 1102 58-59 0.6164003779786995 1102 60-61 0.48551372857726705 1102 62-63 0.5033652087501679 1102 64-65 0.6203509905602473 1102 66-67 0.5644645702032349 1102 68-69 0.5993560030151528 1102 70-71 0.48457206455637447 1102 72-73 0.5274467610900402 1102 74-75 0.6136257030426364 1102 76-77 0.6624256395309445 1102 78-79 0.6571866785800999 1102 80-81 0.5593033665525731 1102 82-83 0.6724836187361554 1102 84-85 0.6695676102742318 1102 86-87 0.9029905680070485 1102 88-89 0.8401274241054963 1102 90-91 1.0918078802473907 1102 92-93 1.0709321214041232 1102 94-95 1.026440684826138 1102 96-97 0.9384782966488174 1102 98-99 0.705191735908528 1102 100-101 0.07326655774764035 1103 1 0.06315057016053771 1103 2 0.013381064984173463 1103 3 0.08711449047299169 1103 4 0.01305167766440718 1103 5 0.11086539563568465 1103 6 0.04683994850716999 1103 7 0.04652661412949044 1103 8 0.10827357114393266 1103 9 0.0905838730426396 1103 10-11 0.16042663045122652 1103 12-13 0.14703621332866135 1103 14-15 0.22805981973159106 1103 16-17 0.18620925371509855 1103 18-19 0.1800418703458888 1103 20-21 0.11087290281394502 1103 22-23 0.14980553511382766 1103 24-25 0.0576407139847106 1103 26-27 0.22254453729277657 1103 28-29 0.22611320922543143 1103 30-31 0.19545895357664023 1103 32-33 0.16115253733872947 1103 34-35 0.14655725401478747 1103 36-37 0.23620062627960436 1103 38-39 0.19464777508643039 1103 40-41 0.22634485320432418 1103 42-43 0.1998072093859875 1103 44-45 0.2884217257323556 1103 46-47 0.1999308698933575 1103 48-49 0.25043014666529473 1103 50-51 0.2774767886717342 1103 52-53 0.2881334714695569 1103 54-55 0.23639978915591797 1103 56-57 0.3291527289194178 1103 58-59 0.29423410005639283 1103 60-61 0.35109631646125905 1103 62-63 0.32879982280391573 1103 64-65 0.29725164608575483 1103 66-67 0.4036367049223841 1103 68-69 0.25082292556958663 1103 70-71 0.20008993291300925 1103 72-73 0.19211550604384087 1103 74-75 0.23874120497140439 1103 76-77 0.1556547594735278 1103 78-79 0.393044580770308 1103 80-81 0.2974629770819881 1103 82-83 0.23829576870604186 1103 84-85 0.2788368403040913 1103 86-87 0.4553728553617482 1103 88-89 0.48400486069958504 1103 90-91 0.59019589944538 1103 92-93 0.30702134784557344 1103 94-95 0.17956997009187248 1103 96-97 0.001195283281990811 1103 98-99 0.028799945676691863 1103 100-101 -0.17158495579219846 1104 1 0.10793988337342597 1104 2 0.12271142201582563 1104 3 0.1237914718579205 1104 4 0.10443112436872326 1104 5 0.14571617991961716 1104 6 0.12202259935447302 1104 7 0.10408498393486809 1104 8 0.06252741160685815 1104 9 0.16795282277224288 1104 10-11 0.1128059333016509 1104 12-13 0.08509279087649446 1104 14-15 0.100640458866323 1104 16-17 0.20073071223161776 1104 18-19 0.19628692887137333 1104 20-21 0.22665566724977992 1104 22-23 0.23639654667444177 1104 24-25 0.28569762470063864 1104 26-27 0.23868785499938383 1104 28-29 0.2213101395951611 1104 30-31 0.19741359165021777 1104 32-33 0.24985356162824246 1104 34-35 0.353833193797918 1104 36-37 0.2556036980272012 1104 38-39 0.2341974750483118 1104 40-41 0.34406902240141335 1104 42-43 0.28263746926955946 1104 44-45 0.29791320223373674 1104 46-47 0.1605043175791181 1104 48-49 0.16956308758680194 1104 50-51 0.265888844125854 1104 52-53 0.18982669713458478 1104 54-55 0.2935046455635799 1104 56-57 0.4092742854558651 1104 58-59 0.3572746419433983 1104 60-61 0.3824303869160346 1104 62-63 0.3413177578342612 1104 64-65 0.34646174188871015 1104 66-67 0.34656641081279105 1104 68-69 0.24306337827964342 1104 70-71 0.36568887885633217 1104 72-73 0.245825472938769 1104 74-75 0.27694484354069004 1104 76-77 0.27113798399270905 1104 78-79 0.4871697840962632 1104 80-81 0.40581041037986765 1104 82-83 0.45661358429407173 1104 84-85 0.4057655566541136 1104 86-87 0.3918674349970175 1104 88-89 0.41193826569000436 1104 90-91 0.5276839894642826 1104 92-93 0.6392347018457052 1104 94-95 0.5466187733022458 1104 96-97 0.5663443588806345 1104 98-99 0.5822260969437245 1104 100-101 0.17742038174756658 1105 1 0.0502023241876941 1105 2 0.11277796597562428 1105 3 0.09057555321281541 1105 4 0.07477741248588643 1105 5 0.07363127537622915 1105 6 0.07291174577630244 1105 7 0.06742690435719112 1105 8 0.14485881822704982 1105 9 0.11924994561903901 1105 10-11 0.12344755508942029 1105 12-13 0.06391957685346483 1105 14-15 0.16723008989657018 1105 16-17 0.12643744281110258 1105 18-19 0.07610894411047298 1105 20-21 0.07996803516946471 1105 22-23 0.14304775837489103 1105 24-25 0.02401191813749648 1105 26-27 0.13489978866113006 1105 28-29 0.15830170140486644 1105 30-31 0.16948454815386782 1105 32-33 0.1548841546277302 1105 34-35 0.2701554201263221 1105 36-37 0.21048978754102166 1105 38-39 0.2728619494957485 1105 40-41 0.19139457174189545 1105 42-43 0.21995149540636305 1105 44-45 0.22872965440257786 1105 46-47 0.21680035508006057 1105 48-49 0.34266752955159774 1105 50-51 0.3143986463238093 1105 52-53 0.3249579372752791 1105 54-55 0.29219557793248185 1105 56-57 0.30715617172118925 1105 58-59 0.3055936415355731 1105 60-61 0.34469219386440386 1105 62-63 0.2628923255795783 1105 64-65 0.24617997055643315 1105 66-67 0.11842103067862553 1105 68-69 0.06566763427057509 1105 70-71 0.2749276537490246 1105 72-73 0.28706525185112497 1105 74-75 0.18939884341391178 1105 76-77 0.20097605517465666 1105 78-79 0.4107011138153638 1105 80-81 0.25381377764296786 1105 82-83 0.39626229999803897 1105 84-85 0.34164179852298915 1105 86-87 0.4402405972343999 1105 88-89 0.3671553408413857 1105 90-91 0.4244197594738175 1105 92-93 0.5336381742348699 1105 94-95 0.3459992441330506 1105 96-97 0.34303153350112225 1105 98-99 0.42962826332993487 1105 100-101 0.0923828610721742 1106 1 0.0499612434697525 1106 2 0.04179653214981727 1106 3 0.06255284343736633 1106 4 0.0458084150998701 1106 5 0.04433637518449274 1106 6 0.1089659237009144 1106 7 0.08972215060050104 1106 8 0.0915347505091546 1106 9 0.15999709576597354 1106 10-11 0.0975360980770219 1106 12-13 0.12693337044666464 1106 14-15 0.027633293522562496 1106 16-17 0.031615366298765935 1106 18-19 -0.02869170425717016 1106 20-21 0.11134685343152029 1106 22-23 0.12811209635117393 1106 24-25 0.12801746510751855 1106 26-27 0.11163796513842072 1106 28-29 0.13152279059937655 1106 30-31 0.15613880712443517 1106 32-33 0.1798445334133092 1106 34-35 0.02851589089172535 1106 36-37 0.12340089286581701 1106 38-39 0.273910953554811 1106 40-41 0.09074802171827656 1106 42-43 0.11377446071891484 1106 44-45 0.13141399167999168 1106 46-47 0.2678011852656752 1106 48-49 0.1974122404294576 1106 50-51 0.22357004161052174 1106 52-53 0.21602834129894433 1106 54-55 0.1386149727142083 1106 56-57 0.2173047118231537 1106 58-59 0.184123339411137 1106 60-61 0.13610974848588597 1106 62-63 0.19763039440890395 1106 64-65 0.14471753631467976 1106 66-67 0.10153016317129726 1106 68-69 0.07614893903154751 1106 70-71 0.02329292640271774 1106 72-73 -0.04188037014558432 1106 74-75 -0.10144778247051534 1106 76-77 -0.04204838485866702 1106 78-79 0.16044685890272703 1106 80-81 0.12063471624304967 1106 82-83 0.045556853574602485 1106 84-85 0.13204486970252916 1106 86-87 0.26849031177003013 1106 88-89 0.4536724613766694 1106 90-91 0.5579074633708991 1106 92-93 0.35990154428123233 1106 94-95 0.17159439366227147 1106 96-97 0.2549555605269589 1106 98-99 0.2702157168750503 1106 100-101 -0.030333071728442462 1107 1 -0.060357250675451724 1107 2 -0.08831921551288957 1107 3 -0.061899285101397084 1107 4 0.0010213471717648304 1107 5 -0.05462117591669369 1107 6 0.06334594285063133 1107 7 -0.030038135496596396 1107 8 -0.008015061269787793 1107 9 0.013519691865759853 1107 10-11 0.04477939628277028 1107 12-13 0.004929153841850109 1107 14-15 0.07781508371101609 1107 16-17 0.09463942532152458 1107 18-19 0.05467872682177699 1107 20-21 0.11081640734989406 1107 22-23 -0.015469324055217726 1107 24-25 -0.007356298162399355 1107 26-27 0.029460077156443276 1107 28-29 -0.02045658422248664 1107 30-31 -8.991857306952511E-4 1107 32-33 0.09300648549803014 1107 34-35 -0.001764160911200463 1107 36-37 0.05971709616343901 1107 38-39 0.12334846095301089 1107 40-41 0.10387550053751227 1107 42-43 0.03338380325698154 1107 44-45 0.0830439691031657 1107 46-47 0.08947699581883484 1107 48-49 0.07209551646013779 1107 50-51 0.05875823975481609 1107 52-53 0.1345999131639033 1107 54-55 0.16831314491659555 1107 56-57 0.12583501823210952 1107 58-59 0.05402208481076798 1107 60-61 0.07366325484975533 1107 62-63 -0.07875641639206066 1107 64-65 0.06280726441404738 1107 66-67 0.2039237834196186 1107 68-69 0.12868817748111638 1107 70-71 0.09948767379336232 1107 72-73 -0.011252147798550993 1107 74-75 0.08965113139916525 1107 76-77 0.12862488327431265 1107 78-79 0.4273211650894986 1107 80-81 0.37623119541308014 1107 82-83 0.2934325750931741 1107 84-85 0.4292683899339025 1107 86-87 0.2765172437896126 1107 88-89 0.4278649686117433 1107 90-91 0.4132298281748952 1107 92-93 0.36592762478284513 1107 94-95 0.22384552351445208 1107 96-97 0.30333812128794335 1107 98-99 0.4812429959192137 1107 100-101 -0.04345542741866382 1108 1 -0.022804482209799914 1108 2 3.561286337117053E-5 1108 3 0.002488756451931806 1108 4 -0.013183500647897972 1108 5 0.052232143973256484 1108 6 0.03883635016529752 1108 7 0.03964297709188003 1108 8 0.02274627392360884 1108 9 -0.0629788131466924 1108 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-0.6041399602783777 2316 94-95 -0.2575256236509631 2316 96-97 -0.6407010583134891 2316 98-99 -0.5017749722463236 2316 100-101 -0.112222460236719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 19.0 5 47.0 6 124.0 7 224.0 8 629.0 9 1154.0 10 2013.0 11 3177.0 12 5030.0 13 6955.0 14 8613.0 15 11548.0 16 15173.0 17 21855.0 18 21193.0 19 14031.0 20 12439.0 21 13185.0 22 14110.0 23 15832.0 24 18133.0 25 21084.0 26 25169.0 27 30437.0 28 37520.0 29 45848.0 30 56332.0 31 69592.0 32 87475.0 33 114687.0 34 158549.0 35 231340.0 36 379932.0 37 681449.0 38 652135.0 39 59130.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.10028596425995 19.73772517076308 15.039179898513291 23.122808966463676 2 15.234616714953464 20.997334794454087 40.07585557893208 23.69219291166037 3 19.261337172301186 24.70704423098824 30.18240386096928 25.849214735741295 4 13.02269708646739 17.347283087562527 37.33121216961271 32.298807656357376 5 13.838407237342917 37.484834349312926 34.257569779622514 14.419188633721639 6 31.5416622152551 36.3370929690968 17.683016480976693 14.438228334671402 7 27.97330492892021 32.92570743948108 21.114111626945974 17.98687600465274 8 25.486684726175607 35.39596760289107 20.152218883222815 18.96512878771051 9 25.782752075944433 17.59077970748673 21.004562829073905 35.621905387494934 10-11 24.679278000436504 26.862179714326917 27.440792700319687 21.01774958491689 12-13 25.809566321448678 24.222201772173054 27.83412118910689 22.134110717271373 14-15 22.580586016977612 25.60654042377571 26.576500896551007 25.236372662695665 16-17 22.020285467570297 28.76025300771935 27.10512147057299 22.11434005413736 18-19 21.786048299163223 27.60126966665521 29.272427413305408 21.340254620876163 20-21 22.708739469546877 26.549135297877886 29.19843916762658 21.54368606494865 22-23 22.25458971383682 26.645920444372518 29.016892093200042 22.082597748590622 24-25 22.29206612024825 26.664817926736305 29.14912244205734 21.893993510958104 26-27 22.078323894468056 26.872699783438513 29.19451255330297 21.854463768790463 28-29 21.76190219305622 27.364263680113275 29.12210407030022 21.751730056530285 30-31 22.19559911942192 26.735509062383915 28.944253032738892 22.124638785455264 32-33 21.806257644528078 26.902022051499568 29.00331749159697 22.28840281237538 34-35 22.583545790180793 26.803591137816614 28.797335561926324 21.815527510076276 36-37 22.338433214829863 26.669347102307995 28.880829947519892 22.11138973534225 38-39 22.032909947819757 27.169980067401244 28.952240759836872 21.84486922494213 40-41 22.344295347572228 26.826716835153675 28.878411652536208 21.95057616473789 42-43 22.073961704743823 26.951131506849794 28.455017505108195 22.519889283298188 44-45 22.31081836069759 26.94936921089084 28.38738749697868 22.3524249314329 46-47 22.494376159145883 27.202306656229947 28.05288173362387 22.250435451000303 48-49 22.5389298450951 27.247621299154716 27.95851220638331 22.25493664936686 50-51 22.1308923487877 27.42458336536 28.141886722201498 22.3026375636508 52-53 22.579876862453627 27.468039735164716 27.656620987822343 22.29546241455931 54-55 22.12908635174296 27.17568249394696 27.96055890689551 22.734672247414565 56-57 22.424060681642448 27.47187324918491 27.71928358541545 22.384782483757196 58-59 22.715511724315938 27.120420841958946 27.75553423466403 22.408533199061083 60-61 22.474875122735078 27.210311280307785 28.191804003301264 22.123009593655876 62-63 22.244722233192103 27.259270647041234 28.13383271369173 22.362174406074935 64-65 22.023695770063163 27.298123702892475 27.893162414257798 22.785018112786563 66-67 22.055582441470246 27.426065938366907 27.5944863600262 22.92386526013665 68-69 22.07548936141705 27.187812988297004 27.86969003040653 22.86700761987942 70-71 22.253712169385672 27.078091170918896 27.80124734744305 22.866949312252384 72-73 22.268111427381697 26.91824105601233 27.966676292293258 22.84697122431271 74-75 22.09587265119726 27.463005684761082 27.546815627645504 22.894306036396152 76-77 22.099887830204363 26.98965348914494 27.761167555244377 23.149291125406325 78-79 22.049242464309263 27.201631072890553 27.695307403999053 23.053819058801132 80-81 22.101501654400877 27.132826670126853 27.76518015057446 23.000491524897818 82-83 22.237038813042986 27.112028235360487 27.728724754597767 22.92220819699876 84-85 22.0169460390194 27.069571419857493 27.671173081811833 23.24230945931128 86-87 22.036968829202753 27.024832900447265 27.76530721556985 23.172891054780134 88-89 21.963653047179672 27.010637303630446 27.515987108358324 23.509722540831557 90-91 21.992188716752135 26.875692000124594 27.648024313096958 23.484094970026305 92-93 22.21746229698478 26.83920866771808 27.687533121146444 23.255795914150692 94-95 21.900909639342228 26.621503790839718 27.79847463788428 23.679111931933775 96-97 21.800016853661212 26.51334771183342 27.947759996812614 23.738875437692755 98-99 21.724448760185318 26.653560777108382 27.498938253701038 24.123052209005266 100-101 21.741974917092847 25.92561512428184 28.25510739982607 24.077302558799246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 334.0 1 232.5 2 174.0 3 239.5 4 241.5 5 233.0 6 402.0 7 900.5 8 1505.0 9 1871.0 10 1908.5 11 1890.0 12 2083.0 13 2304.0 14 2511.0 15 2707.5 16 2904.0 17 3110.0 18 3398.0 19 3758.5 20 4191.0 21 4916.5 22 5999.0 23 7463.0 24 9142.0 25 11416.0 26 14887.0 27 19009.5 28 22705.5 29 26794.5 30 31916.5 31 37736.5 32 44111.0 33 49563.5 34 54985.5 35 60305.0 36 66136.5 37 72960.0 38 77306.5 39 79197.5 40 81669.5 41 87215.5 42 93636.5 43 98963.0 44 104103.5 45 108800.5 46 110587.5 47 117206.5 48 132175.0 49 132763.0 50 120708.5 51 112364.5 52 103712.5 53 98288.5 54 94500.5 55 85950.5 56 76113.0 57 66292.0 58 57259.0 59 48764.5 60 40014.0 61 31834.0 62 25036.0 63 19124.0 64 14029.0 65 10453.5 66 7673.0 67 5730.0 68 4111.5 69 2870.5 70 2281.5 71 1867.5 72 1558.0 73 1419.5 74 1220.0 75 947.0 76 642.5 77 374.0 78 215.0 79 145.0 80 104.5 81 65.0 82 32.5 83 20.0 84 15.0 85 11.0 86 9.5 87 6.0 88 3.0 89 2.0 90 2.0 91 1.5 92 1.0 93 0.5 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3385540898511695 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011459079275320774 16-17 0.0021155223277515276 18-19 5.288805819378819E-5 20-21 0.0 22-23 0.0 24-25 4.58363171012831E-4 26-27 0.0011635372802633402 28-29 0.0 30-31 0.003085136727970978 32-33 0.0 34-35 0.010894939987920368 36-37 0.028876879773808352 38-39 0.02196617350315336 40-41 0.014156370243203974 42-43 0.010313171347788697 44-45 0.0031203954334335033 46-47 0.0013750895130384931 48-49 6.875447565192466E-4 50-51 3.525870546252546E-5 52-53 3.525870546252546E-5 54-55 0.0 56-57 0.0 58-59 0.00528880581937882 60-61 0.010383688758713748 62-63 0.004618890415590835 64-65 0.002150781033214053 66-67 0.004283932713696844 68-69 0.0038960869536090632 70-71 1.762935273126273E-5 72-73 0.0 74-75 0.0 76-77 0.21488418044136143 78-79 3.1732834916272913E-4 80-81 0.0037726814844902244 82-83 6.699154037879838E-4 84-85 0.0 86-87 0.10797978547898422 88-89 0.4020550183891778 90-91 0.38907981477896847 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0017453059203950102 100-101 8.638382838318737E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2836179.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.558406661334864 #Duplication Level Percentage of deduplicated Percentage of total 1 70.67049012761638 35.02316888700264 2 15.52338415615964 15.38628369542164 3 5.901381184171964 8.773891457662323 4 2.775523939006553 5.50202176270227 5 1.5052851343481224 3.7299766414643165 6 0.869515729157144 2.5855088462398115 7 0.5796914951760929 2.0110010799237834 8 0.4094637546806391 1.6233897014032133 9 0.27963139450574653 1.2472277727772751 >10 1.325691292590954 11.68595890056518 >50 0.09693768151949146 3.30729463762526 >100 0.05730372477490656 5.510651491136116 >500 0.004061525233310112 1.4508599649288645 >1k 0.0014250965730664457 1.3471110826413089 >5k 2.1376448595996682E-4 0.8156540785060541 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8598 0.30315434956679393 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7540 0.265850639187442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6960 0.24540059001917722 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4470 0.1576064134174888 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3828 0.13497032451054747 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3145 0.11088862867964257 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.525870546252546E-5 0.0 0.0 0.0 6 0.0 3.525870546252546E-5 0.0 0.0 0.0 7 0.0 3.525870546252546E-5 0.0 0.0 0.0 8 0.0 3.525870546252546E-5 0.0 0.0 0.0 9 0.0 3.525870546252546E-5 0.0 0.0 0.0 10-11 0.0 3.525870546252546E-5 0.0 0.0 0.0 12-13 0.0 3.525870546252546E-5 0.0 0.0 0.0 14-15 0.0 3.525870546252546E-5 0.0 1.762935273126273E-5 0.0 16-17 0.0 3.525870546252546E-5 0.0 3.525870546252546E-5 0.0 18-19 0.0 7.051741092505092E-5 0.0 3.525870546252546E-5 0.0 20-21 0.0 1.0577611638757639E-4 0.0 5.2888058193788193E-5 0.0 22-23 0.0 1.234054691188391E-4 0.0 1.234054691188391E-4 0.0 24-25 0.0 1.4103482185010184E-4 0.0 3.3495770189399186E-4 0.0 26-27 0.0 1.4103482185010184E-4 0.0 7.933208729068229E-4 0.0 28-29 0.0 1.4103482185010184E-4 0.0 0.0026796616151519353 0.0 30-31 0.0 1.4103482185010184E-4 0.0 0.008990969892943993 0.0 32-33 0.0 1.5866417458136457E-4 0.0 0.021525439684871795 0.0 34-35 0.0 1.762935273126273E-4 0.0 0.04262777490419328 0.0 36-37 0.0 1.762935273126273E-4 0.0 0.07622932120998005 0.0 38-39 0.0 2.1155223277515277E-4 0.0 0.12619090685037865 0.0 40-41 0.0 2.1155223277515277E-4 0.0 0.18165285054293118 0.0 42-43 0.0 2.1155223277515277E-4 0.0 0.24138109759644932 0.0 44-45 0.0 2.1155223277515277E-4 0.0 0.31739886657365424 0.0 46-47 0.0 2.291815855064155E-4 0.0 0.4078021873795695 0.0 48-49 0.0 2.468109382376782E-4 0.0 0.5021721125500189 0.0 50-51 0.0 2.468109382376782E-4 0.0 0.6052333086169808 0.0 52-53 0.0 2.820696437002037E-4 0.0 0.711732228466539 0.0 54-55 0.0 2.996989964314664E-4 0.0 0.8318938966828258 0.0 56-57 0.0 3.3495770189399186E-4 0.0 0.9791518800470633 0.0 58-59 0.0 3.525870546252546E-4 0.0 1.140037353072567 0.0 60-61 0.0 3.525870546252546E-4 0.0 1.3136688481227736 0.0 62-63 0.0 3.525870546252546E-4 0.0 1.493594022098041 0.0 64-65 0.0 3.525870546252546E-4 0.0 1.6888919916549696 0.0 66-67 0.0 3.525870546252546E-4 0.0 1.9163282712409901 0.0 68-69 0.0 3.525870546252546E-4 0.0 2.17447488328487 0.0 70-71 0.0 3.7021640735651737E-4 0.0 2.457126295625206 0.0 72-73 0.0 3.878457600877801E-4 0.0 2.74716440675994 0.0 74-75 0.0 3.878457600877801E-4 0.0 3.056365624313557 0.0 76-77 0.0 3.878457600877801E-4 0.0 3.3940558758808947 0.0 78-79 0.0 3.878457600877801E-4 0.0 3.779169086295329 0.0 80-81 0.0 3.878457600877801E-4 0.0 4.1973902211390755 0.0 82-83 0.0 4.054751128190428E-4 0.0 4.627211470079992 0.0 84-85 0.0 4.2310446555030555E-4 0.0 5.079827472102431 0.0 86-87 0.0 4.58363171012831E-4 0.0 5.559768970858327 0.0 88-89 0.0 4.58363171012831E-4 0.0 6.080663456008947 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10770 0.0 22.100225 1 TACATGG 10885 0.0 21.273458 2 GTATCAA 18475 0.0 21.231722 1 GAGTACT 9215 0.0 21.062279 12-13 ACATGGG 10900 0.0 20.547653 3 GTACTTT 9580 0.0 20.185497 14-15 AGTACTT 9520 0.0 19.739473 12-13 GGTATCA 14365 0.0 19.717588 1 AGAGTAC 15215 0.0 19.461771 10-11 ATCAACG 21110 0.0 18.364561 3 CAACGCA 21235 0.0 18.30115 5 TCAACGC 21210 0.0 18.300348 4 TATCAAC 21540 0.0 18.130127 2 AACGCAG 21640 0.0 17.914782 6 CATGGGG 5240 0.0 17.115013 4 ATAGCGT 250 4.609934E-6 17.082415 6 TACTTTT 11275 0.0 16.624857 14-15 ACTTTTT 11710 0.0 16.574188 16-17 GGACCGT 315 1.3576027E-7 16.570242 6 GTGTAGG 1185 0.0 16.470545 1 >>END_MODULE