FastQCFastQC Report
Fri 27 May 2016
SRR939094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791199
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80030.28672265933027347No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71410.25583987383199835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61940.2219118020606915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40660.1456721645429079No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39310.14083553340338686No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31170.11167243897694146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG102450.023.6356721
TACATGG104100.022.7939932
ACATGGG103650.022.345423
GAGTACT81050.021.81723212-13
GTATCAA175750.021.0991761
CGTACAC2351.1681004E-720.1549223
GTACTTT89050.019.99050514-15
CATGGGG53350.019.7979154
GGTATCA138000.019.7488841
AGTACTT85500.019.62963312-13
AGAGTAC138850.019.05971110-11
CGACGGT1751.0200752E-418.9367947
ATCAACG198500.018.4684073
CAACGCA199000.018.4216755
TCAACGC199500.018.3995764
TATCAAC203150.018.1382832
AACGCAG202950.018.0083836
ACTTTTT108900.016.56480816-17
GAGTACA86000.016.5627561
ACGCAGA220550.016.549847
Produced by FastQC (version 0.11.5)