##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939094_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2791199 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26261617319295 33.0 31.0 34.0 31.0 34.0 2 32.366981716459485 34.0 31.0 34.0 31.0 34.0 3 32.434389665516505 34.0 31.0 34.0 31.0 34.0 4 35.86353355672598 37.0 35.0 37.0 35.0 37.0 5 35.90160285955964 37.0 35.0 37.0 35.0 37.0 6 35.98769668518798 37.0 37.0 37.0 35.0 37.0 7 35.92715281139037 37.0 37.0 37.0 35.0 37.0 8 35.92553271909312 37.0 37.0 37.0 35.0 37.0 9 37.63692520669433 39.0 38.0 39.0 35.0 39.0 10-11 37.548242887733906 39.0 38.0 39.0 35.0 39.0 12-13 37.46637090368691 39.0 38.0 39.0 35.0 39.0 14-15 38.7982209437593 40.0 38.0 41.0 35.0 41.0 16-17 38.71855374697397 40.0 38.0 41.0 35.0 41.0 18-19 38.621798911507206 40.0 38.0 41.0 34.0 41.0 20-21 38.61252422346095 40.0 38.5 41.0 34.0 41.0 22-23 38.65549267536998 40.0 39.0 41.0 34.0 41.0 24-25 38.611034541069984 40.5 38.0 41.0 34.0 41.0 26-27 38.51602196762036 40.0 38.0 41.0 34.0 41.0 28-29 38.387460550107676 40.0 38.0 41.0 34.0 41.0 30-31 38.19800648395188 40.0 38.0 41.0 34.0 41.0 32-33 38.0227636940254 40.0 38.0 41.0 33.5 41.0 34-35 37.73356736656899 40.0 38.0 41.0 33.0 41.0 36-37 37.483442241130064 40.0 37.5 41.0 32.5 41.0 38-39 37.31994314987932 40.0 37.0 41.0 32.0 41.0 40-41 37.15767095072763 40.0 37.0 41.0 31.0 41.0 42-43 36.967630577397024 40.0 37.0 41.0 31.0 41.0 44-45 36.72077555201188 40.0 37.0 41.0 30.5 41.0 46-47 36.609076959399886 40.0 37.0 41.0 30.0 41.0 48-49 36.513356983862494 40.0 37.0 41.0 30.0 41.0 50-51 35.402240220063135 38.5 35.0 40.0 28.5 40.5 52-53 35.55600102321618 38.5 35.5 40.0 28.5 40.5 54-55 36.10875039723072 39.5 36.0 41.0 29.0 41.0 56-57 36.03308291526329 39.0 36.0 41.0 29.0 41.0 58-59 35.848787564054014 39.0 35.0 41.0 28.0 41.0 60-61 35.566958500630015 39.0 35.0 41.0 28.0 41.0 62-63 35.21256420627838 38.5 35.0 40.5 27.5 41.0 64-65 34.85202327028635 38.0 35.0 40.0 26.5 41.0 66-67 34.47232820017491 37.0 34.0 40.0 26.0 41.0 68-69 34.0702875359299 37.0 34.0 39.5 26.0 41.0 70-71 33.63672851702799 36.0 34.0 39.0 25.0 41.0 72-73 33.18880846546592 36.0 33.5 39.0 24.5 40.0 74-75 32.74309893346909 35.0 33.0 37.5 23.5 40.0 76-77 32.3024929429969 35.0 33.0 37.0 22.0 39.0 78-79 31.867836546229775 35.0 32.0 36.5 20.5 39.0 80-81 31.414244738551425 35.0 32.0 36.0 19.5 38.5 82-83 31.024620781248487 35.0 31.5 36.0 18.0 37.0 84-85 30.647271477239713 35.0 31.0 35.0 13.0 37.0 86-87 30.258202299441926 34.0 31.0 35.0 7.0 36.0 88-89 29.855106353936065 34.0 31.0 35.0 3.5 36.0 90-91 29.462588120732345 34.0 30.0 35.0 2.0 36.0 92-93 29.072730751193305 34.0 30.0 35.0 2.0 35.0 94-95 28.609877869689694 34.0 29.0 35.0 2.0 35.0 96-97 28.049028929861326 34.0 29.0 35.0 2.0 35.0 98-99 27.382296640261046 34.0 28.0 35.0 2.0 35.0 100-101 24.447080089954177 31.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2433640732040132 1101 2 0.2652263554875063 1101 3 0.2131687796366606 1101 4 0.23307451595572815 1101 5 0.19604045611460919 1101 6 0.18339110684495097 1101 7 0.10360126190139596 1101 8 0.23487439239698915 1101 9 0.22128036218969527 1101 10-11 0.21692835164277113 1101 12-13 0.2685375792575897 1101 14-15 0.3270088936698201 1101 16-17 0.3777695056320809 1101 18-19 0.4316471548537777 1101 20-21 0.4694565617951838 1101 22-23 0.3800220980739155 1101 24-25 0.35381590628868764 1101 26-27 0.3822920475664091 1101 28-29 0.3506444639328592 1101 30-31 0.24451202255105642 1101 32-33 0.22129266106973944 1101 34-35 0.23792630186336083 1101 36-37 0.2595425240461253 1101 38-39 0.15366962542408658 1101 40-41 0.2723415640076965 1101 42-43 0.2054173219878095 1101 44-45 0.10350953338011948 1101 46-47 0.22925513669007813 1101 48-49 0.36040275125601795 1101 50-51 0.3581745835500172 1101 52-53 0.30482559636261897 1101 54-55 0.37080166045851826 1101 56-57 0.3342280019767969 1101 58-59 0.28927654592385466 1101 60-61 0.18916186970169235 1101 62-63 0.07469761169007683 1101 64-65 -0.0033194939305332127 1101 66-67 0.06406543542509269 1101 68-69 0.10909339367089643 1101 70-71 -0.04991155975462647 1101 72-73 -0.015480626615193671 1101 74-75 -0.01844118799544958 1101 76-77 -0.05292411819836218 1101 78-79 -0.1031257668130614 1101 80-81 -0.25171766079513347 1101 82-83 -0.29520719488688485 1101 84-85 -0.23984729874848654 1101 86-87 -0.23302607266778708 1101 88-89 -0.28390902388442996 1101 90-91 -0.1736565941413808 1101 92-93 -0.405184765956502 1101 94-95 -0.3246141572924799 1101 96-97 -0.354836404444022 1101 98-99 -0.4913583457135289 1101 100-101 -0.8230227052957595 1102 1 0.07661702605581411 1102 2 0.16157015707367606 1102 3 0.2068857053303006 1102 4 0.09873158985566022 1102 5 0.17171961898842625 1102 6 0.18154287772091493 1102 7 0.18589539043779268 1102 8 0.17943708778071255 1102 9 0.30164153152736617 1102 10-11 0.2595271361228697 1102 12-13 0.15348727360297687 1102 14-15 0.31687964811892755 1102 16-17 0.26401250800660847 1102 18-19 0.30154856175578715 1102 20-21 0.31329016097565443 1102 22-23 0.37823284943308266 1102 24-25 0.4057065526451922 1102 26-27 0.31181358883952726 1102 28-29 0.2778329704287188 1102 30-31 0.3273408030782363 1102 32-33 0.3278777885232529 1102 34-35 0.2747286445194419 1102 36-37 0.2900523972746285 1102 38-39 0.17233503453638122 1102 40-41 0.13353813012569304 1102 42-43 0.025360432788993137 1102 44-45 -0.012708203077686164 1102 46-47 0.07871397770792754 1102 48-49 0.1846473487133551 1102 50-51 0.04690517044584652 1102 52-53 0.06833847742178278 1102 54-55 0.1036618276499297 1102 56-57 0.1243670915269135 1102 58-59 0.05134701490047178 1102 60-61 -0.04443877222866632 1102 62-63 -0.046641161766402206 1102 64-65 0.048055290677616824 1102 66-67 0.09063849188768103 1102 68-69 -0.010332515707240475 1102 70-71 -0.14433496376710764 1102 72-73 -0.09915923065329224 1102 74-75 -0.18936123481794453 1102 76-77 -0.26505499432472845 1102 78-79 -0.24210770799820835 1102 80-81 -0.1146600666142632 1102 82-83 -0.2915140423296023 1102 84-85 -0.3614152957643171 1102 86-87 -0.38926354583949774 1102 88-89 -0.4513126856155232 1102 90-91 -0.40433961597424783 1102 92-93 -0.5366577435000721 1102 94-95 -0.6527874812724512 1102 96-97 -0.6220304331833475 1102 98-99 -0.5995396868636007 1102 100-101 -0.9921448042911081 1103 1 0.1830449524340736 1103 2 0.16574148243705622 1103 3 0.21931905013119746 1103 4 0.27954188055834095 1103 5 0.2890058923177463 1103 6 0.2592230617752662 1103 7 0.2756783310071569 1103 8 0.27020910549809685 1103 9 0.36719745738957954 1103 10-11 0.39693763539181504 1103 12-13 0.44269155028655405 1103 14-15 0.4622686134582281 1103 16-17 0.4917663353051367 1103 18-19 0.4416830547462425 1103 20-21 0.41952860382419743 1103 22-23 0.4626175251877882 1103 24-25 0.5353079115511861 1103 26-27 0.5306724410800001 1103 28-29 0.5481198028016436 1103 30-31 0.6042568712815566 1103 32-33 0.48728315964220315 1103 34-35 0.48074427623871685 1103 36-37 0.4404942567396617 1103 38-39 0.4233818974416863 1103 40-41 0.43940486273083224 1103 42-43 0.40756080691817687 1103 44-45 0.4711991354447207 1103 46-47 0.5445397496181315 1103 48-49 0.5524698201161229 1103 50-51 0.4753287749869983 1103 52-53 0.5273818140520348 1103 54-55 0.4563981848357841 1103 56-57 0.596194519066259 1103 58-59 0.5435136851507423 1103 60-61 0.5528012187406262 1103 62-63 0.43166902142788643 1103 64-65 0.4504013924235508 1103 66-67 0.4425430029395443 1103 68-69 0.4914171832692773 1103 70-71 0.42730862443277573 1103 72-73 0.37935925003819904 1103 74-75 0.3555262039022722 1103 76-77 0.3245142733281483 1103 78-79 0.374911138221794 1103 80-81 0.37951969815738806 1103 82-83 0.21160907627365333 1103 84-85 0.2485510003567839 1103 86-87 0.20614745871885987 1103 88-89 0.10985286654754489 1103 90-91 0.2684989279321748 1103 92-93 0.2590198818547016 1103 94-95 0.17730895374948474 1103 96-97 0.2275613037221298 1103 98-99 0.17024857537085225 1103 100-101 0.16668405310976908 1104 1 0.18484478303825114 1104 2 0.2626231358853275 1104 3 0.23945163991874807 1104 4 0.2431190268862835 1104 5 0.2505023386317049 1104 6 0.25366395195808167 1104 7 0.21986269919182178 1104 8 0.2819439427589643 1104 9 0.32473696365515536 1104 10-11 0.28101473574459135 1104 12-13 0.2928847916781976 1104 14-15 0.4125051913000064 1104 16-17 0.3700977540857977 1104 18-19 0.34181512002837877 1104 20-21 0.4161663460679961 1104 22-23 0.3478061237352321 1104 24-25 0.3460547036501893 1104 26-27 0.3616275039170418 1104 28-29 0.41665090273482264 1104 30-31 0.4244582781216266 1104 32-33 0.3924969376870564 1104 34-35 0.4773375812185421 1104 36-37 0.5329404398943609 1104 38-39 0.22650612039663542 1104 40-41 0.25956992652623256 1104 42-43 0.4705804557974176 1104 44-45 0.5425008704568199 1104 46-47 0.4703201294895578 1104 48-49 0.4570024696066355 1104 50-51 0.3846416275711917 1104 52-53 0.40140200880542665 1104 54-55 0.34122545743355204 1104 56-57 0.3388252180441924 1104 58-59 0.33039135300543165 1104 60-61 0.3213373856550845 1104 62-63 0.39199002521468884 1104 64-65 0.3817056722581995 1104 66-67 0.2012075409344547 1104 68-69 0.12094428012535019 1104 70-71 0.1920083742242653 1104 72-73 0.156028974161309 1104 74-75 0.11461131753216591 1104 76-77 0.04654817904149411 1104 78-79 0.09788559221813742 1104 80-81 0.05939810031964754 1104 82-83 0.023630869797237608 1104 84-85 0.02487461566249749 1104 86-87 0.05892267512590621 1104 88-89 0.015574071200486372 1104 90-91 0.07217655266085998 1104 92-93 -0.04068380496505952 1104 94-95 -0.07493427818810616 1104 96-97 -0.10586022962087327 1104 98-99 0.00957565111720271 1104 100-101 0.010495650118663491 1105 1 0.09095891347303109 1105 2 0.1263492340604344 1105 3 0.15255261344288584 1105 4 0.15168067925963413 1105 5 0.15452435822683697 1105 6 0.20584440917786395 1105 7 0.14123738133183394 1105 8 0.2013069139396535 1105 9 0.2573405174545158 1105 10-11 0.26040151526193966 1105 12-13 0.18524431002681752 1105 14-15 0.2663777855361431 1105 16-17 0.3019251218311112 1105 18-19 0.2595072227657198 1105 20-21 0.22708491470081782 1105 22-23 0.3072451631747981 1105 24-25 0.4078601639862498 1105 26-27 0.4271669621838967 1105 28-29 0.36160651140554023 1105 30-31 0.38142813751532145 1105 32-33 0.38816079762921163 1105 34-35 0.37467690610884574 1105 36-37 0.4451563914799266 1105 38-39 0.28180316367545544 1105 40-41 0.35149394552303903 1105 42-43 0.20630776308701115 1105 44-45 0.3146523619057646 1105 46-47 0.31343433159865697 1105 48-49 0.40220348488885094 1105 50-51 0.2859314535584261 1105 52-53 0.3133573619416481 1105 54-55 0.35753962152409713 1105 56-57 0.4178234760467774 1105 58-59 0.48165796274815165 1105 60-61 0.4211073266951715 1105 62-63 0.3487373047071074 1105 64-65 0.44713434696900123 1105 66-67 0.4635987050499324 1105 68-69 0.4119072400874515 1105 70-71 0.311364022160042 1105 72-73 0.41654725734340303 1105 74-75 0.45665749448669146 1105 76-77 0.35119345352295284 1105 78-79 0.33094046046205605 1105 80-81 0.262284004163881 1105 82-83 0.2780508376372879 1105 84-85 0.203963946785354 1105 86-87 0.17022010482275363 1105 88-89 0.2938575337553324 1105 90-91 0.27949730455555155 1105 92-93 0.2327363997118468 1105 94-95 0.2400144123533785 1105 96-97 0.23331414463121902 1105 98-99 0.1935364203383827 1105 100-101 -0.06165147854607511 1106 1 0.1737119411546928 1106 2 0.08544924967942791 1106 3 0.2373596888465741 1106 4 0.15447587822157516 1106 5 0.15738038115071618 1106 6 0.1769351555254275 1106 7 0.2089206341672778 1106 8 0.2446324978496861 1106 9 0.2193255412434425 1106 10-11 0.2763331881762028 1106 12-13 0.24421261548625495 1106 14-15 0.28401252938466115 1106 16-17 0.3406601486236909 1106 18-19 0.35478855987163627 1106 20-21 0.3663084855629819 1106 22-23 0.3071897457872552 1106 24-25 0.30679814757429114 1106 26-27 0.30649207151684266 1106 28-29 0.38871620178904465 1106 30-31 0.42220752525080485 1106 32-33 0.32877363283682826 1106 34-35 0.1977443561280694 1106 36-37 0.22254564681441025 1106 38-39 0.32171029074196866 1106 40-41 0.43897186867759785 1106 42-43 0.3491679310512552 1106 44-45 0.16879507444421193 1106 46-47 0.17918030370072557 1106 48-49 0.1751854059048057 1106 50-51 0.2600733100559296 1106 52-53 0.22558019991262057 1106 54-55 0.29027147345605187 1106 56-57 0.3433999520415938 1106 58-59 0.2698507551837537 1106 60-61 0.3132391840336979 1106 62-63 0.2064058546638492 1106 64-65 0.25359280044843757 1106 66-67 0.2311946266906446 1106 68-69 0.2395439963831123 1106 70-71 0.14087595867484737 1106 72-73 0.17463661900718108 1106 74-75 0.20179833574967176 1106 76-77 0.20215116810388167 1106 78-79 0.16477803194898044 1106 80-81 0.12459409823827627 1106 82-83 0.15674818360999154 1106 84-85 0.2306449408741642 1106 86-87 0.390073346364467 1106 88-89 0.3723064776711347 1106 90-91 0.30039912116454914 1106 92-93 0.14697506392753468 1106 94-95 0.2685421270544559 1106 96-97 0.3274993738699763 1106 98-99 0.3711188856161627 1106 100-101 0.44799077142989674 1107 1 0.10388019586131492 1107 2 0.18653966191435956 1107 3 0.06323531974216934 1107 4 0.12714244700278243 1107 5 0.17744126937028426 1107 6 0.11005224573438 1107 7 0.12173385499874456 1107 8 0.20549304095805354 1107 9 0.24226192305909677 1107 10-11 0.15156720010985936 1107 12-13 0.20246114724048425 1107 14-15 0.15996379051173193 1107 16-17 0.12274640245916402 1107 18-19 0.07460349283237377 1107 20-21 0.11803783140660329 1107 22-23 0.25284707478764545 1107 24-25 0.3301528525658597 1107 26-27 0.2681617577621864 1107 28-29 0.22487521974669988 1107 30-31 0.23520501040304254 1107 32-33 0.2806165864482182 1107 34-35 0.2891254838407278 1107 36-37 0.2653748657101147 1107 38-39 0.20413758444151142 1107 40-41 0.25444218881069247 1107 42-43 0.3411517184475059 1107 44-45 0.3631983362533049 1107 46-47 0.3985935886630543 1107 48-49 0.27606366079478306 1107 50-51 0.17562147655880977 1107 52-53 0.2030584597401841 1107 54-55 0.146624564492555 1107 56-57 0.10729633032298125 1107 58-59 0.0654138008536691 1107 60-61 0.05434969021845859 1107 62-63 0.15457225647673312 1107 64-65 0.12609663104711188 1107 66-67 -0.012159881952506169 1107 68-69 -0.04549116514160545 1107 70-71 -0.11751010359098757 1107 72-73 -0.01167037490876055 1107 74-75 0.03501645876569626 1107 76-77 0.003095531771165838 1107 78-79 -0.09501791296547069 1107 80-81 -0.1059742307919933 1107 82-83 -0.12653911612726532 1107 84-85 0.059975581924295085 1107 86-87 -0.0823618633522365 1107 88-89 -0.08587001154132068 1107 90-91 -0.12144735372458015 1107 92-93 -0.10638433465728525 1107 94-95 -0.03624965484057441 1107 96-97 -0.18950346004395868 1107 98-99 -0.4019649379746113 1107 100-101 -0.30367461680649654 1108 1 0.05530186219097999 1108 2 0.112923467119046 1108 3 0.10735601316815746 1108 4 0.1838521536186093 1108 5 0.16485088066274045 1108 6 0.1768637544159617 1108 7 -0.0596425980637747 1108 8 0.08952520606419512 1108 9 0.12984938879151287 1108 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-1.5804810000398888 2316 98-99 -1.9945023559417976 2316 100-101 -1.8347409070605067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7451.0 3 1738.0 4 1696.0 5 1876.0 6 2220.0 7 2644.0 8 3134.0 9 4176.0 10 5155.0 11 6556.0 12 9117.0 13 15847.0 14 12913.0 15 12570.0 16 15767.0 17 21937.0 18 18529.0 19 12755.0 20 12491.0 21 12804.0 22 14128.0 23 15575.0 24 17794.0 25 20346.0 26 23553.0 27 27519.0 28 32970.0 29 39859.0 30 48230.0 31 60044.0 32 75986.0 33 100765.0 34 141509.0 35 213604.0 36 355180.0 37 625599.0 38 677171.0 39 119940.0 40 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.925051713961274 19.22392549880458 14.61986326868204 23.231159518552104 2 15.003504377063798 20.50566331724261 40.740051263620266 23.750781042073328 3 19.3158348201187 24.500788050047813 29.973234276482536 26.210142853350956 4 12.813813647911687 16.81498025873505 37.65053476837466 32.7206713249786 5 13.904010934509534 37.28778961831459 34.32983976710356 14.478359680072323 6 31.57104637229924 35.99856872521173 17.962247617709085 14.468137284779942 7 28.07206580513762 32.78567433054603 21.26661981325569 17.875640051060657 8 25.54702780810596 35.04133298194472 20.423566701984807 18.98807250796451 9 26.04456839494185 17.571273826815833 21.117834923610406 35.2663228546319 10-11 24.749824623112247 26.635666235866097 27.619103450761003 20.995405690260664 12-13 25.84650127031441 24.108758491098474 27.897990463184723 22.146749775402387 14-15 22.700943417366947 25.495681792717086 26.595979831932777 25.207394957983194 16-17 22.20814312206078 28.574788705932296 27.079631294899542 22.137436877107383 18-19 21.92294852178391 27.560992751905616 29.169052756215812 21.347005970094663 20-21 22.829706458451927 26.514390214385287 29.058470751177705 21.597432575985085 22-23 22.387110436352884 26.55485220756693 28.867251918019388 22.190785438060797 24-25 22.4039798946528 26.676940039037138 28.99517379848887 21.923906267821184 26-27 22.157825069971977 26.84768248262559 29.028609468055173 21.96588297934726 28-29 21.867892659625383 27.359791670141043 28.94416090547886 21.82815476475471 30-31 22.26110736962598 26.731847401915115 28.80408627553922 22.202958952919687 32-33 21.836828882225237 26.887331034922973 28.893915715231405 22.381924367620385 34-35 22.69549197691497 26.834522714114158 28.598039386625718 21.871945922345155 36-37 22.432386531354183 26.685584336083274 28.684114362570533 22.197914769992007 38-39 22.13231353861756 27.117641805149006 28.77988006200014 21.970164594233296 40-41 22.45569416539561 26.83849254403311 28.724459643342737 21.981353647228545 42-43 22.106323652677627 26.906184641618868 28.383663319537266 22.60382838616624 44-45 22.382600746915077 26.944534586331574 28.252248254719003 22.420616412034345 46-47 22.52325532816052 27.175077289661193 27.94840608982024 22.35326129235805 48-49 22.555452884205508 27.238064845751953 27.853540669830608 22.35294160021193 50-51 22.227310742464528 27.339934922968652 28.11539899481415 22.317355339752673 52-53 22.62955780847732 27.42255921603317 27.617340742330835 22.330542233158674 54-55 22.153114821169517 27.127803252615795 27.974109305062743 22.744972621151945 56-57 22.458988681212663 27.487282499094917 27.709632859966437 22.34409595972598 58-59 22.71346531620068 27.05476573164052 27.760954055367137 22.47081489679167 60-61 22.525190154087962 27.048029155160986 28.269448468584002 22.15733222216705 62-63 22.356571622408246 27.166286550850028 28.158992900443625 22.318148926298104 64-65 22.064875908328272 27.263949912363927 27.97705109114656 22.69412308816124 66-67 22.135082750977617 27.347276729420418 27.655633022869697 22.86200749673227 68-69 22.157961124367652 27.17032075224936 27.88671439982216 22.785003723560827 70-71 22.284652830417077 27.03443883065341 27.885412133282223 22.79549620564729 72-73 22.334913931059475 26.90459264291706 28.05586479684603 22.704628629177435 74-75 22.205999614908205 27.445661620307053 27.6164132679794 22.731925496805342 76-77 22.27835746782743 27.03050733308663 27.74318845433894 22.947946744747 78-79 22.196794402455136 27.206082897772905 27.724956843016958 22.872165856755004 80-81 22.291647912428207 27.233620798300418 27.71652593486128 22.758205354410098 82-83 22.400316482193105 27.164475486044875 27.792091942614732 22.643116089147284 84-85 22.23234127048432 27.17686475873992 27.678711935183355 22.9120820355924 86-87 22.25356804286783 27.168693951815104 27.80710196504565 22.770636040271416 88-89 22.20330241818145 27.117763535102956 27.597373364565414 23.081560682150172 90-91 22.249572426671353 27.063374508128575 27.699853609266416 22.987199455933652 92-93 22.567810241197144 27.011594249886073 27.75053180088222 22.67006370803456 94-95 22.290085272581383 26.790035292348342 27.840340622042277 23.079538813028 96-97 22.25158439564584 26.67764386089126 27.97505888294501 23.09571286051789 98-99 22.223304898375133 26.788652048977628 27.543683581270173 23.444359471377062 100-101 22.38077488223346 26.166879150640334 28.11786017834248 23.334485788783727 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2327.0 1 1278.5 2 261.5 3 321.0 4 323.5 5 357.0 6 551.5 7 1077.5 8 1833.0 9 2256.5 10 2259.5 11 2354.5 12 2755.5 13 3051.5 14 3253.5 15 3474.0 16 3705.0 17 3947.5 18 4111.0 19 4312.0 20 4709.5 21 5273.0 22 6038.5 23 7401.0 24 8939.5 25 11108.0 26 14514.0 27 18534.0 28 22065.0 29 25854.0 30 30894.5 31 36722.5 32 42751.0 33 48157.5 34 53580.0 35 58920.5 36 65086.5 37 71621.5 38 76172.0 39 78463.0 40 80303.0 41 85568.0 42 92029.0 43 97401.5 44 102468.5 45 106696.5 46 108669.5 47 114619.5 48 129063.5 49 130525.5 50 118937.5 51 110076.0 52 100962.0 53 95285.0 54 91324.5 55 83490.5 56 74184.5 57 64623.0 58 55802.5 59 47378.5 60 38860.0 61 31153.0 62 24865.0 63 19198.5 64 14171.5 65 10570.5 66 7836.5 67 5943.0 68 4359.5 69 3139.0 70 2451.0 71 2015.0 72 1709.0 73 1460.0 74 1208.5 75 991.5 76 736.5 77 482.0 78 331.0 79 227.5 80 160.0 81 126.0 82 94.5 83 61.0 84 38.0 85 28.5 86 24.5 87 21.5 88 17.5 89 15.5 90 11.0 91 8.0 92 6.0 93 4.0 94 3.5 95 3.5 96 2.5 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39416752442230024 2 0.11668820460311143 3 0.11840789567494113 4 0.13030242558842992 5 0.1323445587362277 6 0.07441246575396451 7 0.08451565080096403 8 0.0811120955546344 9 0.08139871073327269 10-11 0.07901622206084195 12-13 0.08320796904842687 14-15 0.07654416614508676 16-17 0.07864003963887921 18-19 0.08834912881525107 20-21 0.08639656291077777 22-23 0.09710880521238364 24-25 0.11052597826238832 26-27 0.11172617932293613 28-29 0.10491906883027687 30-31 0.09476214343728268 32-33 0.09332906754409126 34-35 0.3428454939973825 36-37 0.5359166437075966 38-39 0.5170179553661348 40-41 0.48934167717887544 42-43 0.5173941377880975 44-45 0.5270853135158046 46-47 0.5241833348320919 48-49 0.5306142629027883 50-51 0.5301305997888363 52-53 0.5286258701009853 54-55 0.5231801817068579 56-57 0.4953247690329497 58-59 0.48511410329396076 60-61 0.43483105289160684 62-63 0.5085448941476405 64-65 0.5354329805936445 66-67 0.5441926569907771 68-69 0.5599744052645476 70-71 0.5551377741250265 72-73 0.5424013121242878 74-75 0.5459123480626068 76-77 0.5220516344409697 78-79 0.4957905186982368 80-81 0.5033320805861566 82-83 0.5167492536361614 84-85 0.5108199021280819 86-87 0.5120201031886297 88-89 0.5218904134029856 90-91 0.5398038620678783 92-93 0.5465034918685483 94-95 0.541039890025756 96-97 0.5424371390216176 98-99 0.5429924559302293 100-101 0.5201707223311559 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2791199.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.02971673585576 #Duplication Level Percentage of deduplicated Percentage of total 1 71.96193348567655 36.721970815385674 2 14.929241018064754 15.236698804663263 3 5.655989862537796 8.658706816385262 4 2.6370272923250715 5.382670230082763 5 1.4172654285814499 3.6161326680016286 6 0.8406542625803688 2.5739009335359557 7 0.5447313119093108 1.945823918071825 8 0.37293254966982525 1.522451389698531 9 0.25383059868190433 1.1657613194667233 >10 1.2366670940826565 11.29209118373524 >50 0.08975863695098348 3.1470513374849665 >100 0.05419812136910969 5.179535949643134 >500 0.004222198222179863 1.509495800426822 >1k 0.0013370294369822713 1.2814698623275211 >5k 2.111099111024639E-4 0.7662389710907541 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8003 0.28672265933027347 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7141 0.25583987383199835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6194 0.2219118020606915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4066 0.1456721645429079 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3931 0.14083553340338686 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3117 0.11167243897694146 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.5826897329785515E-5 0.0 5 0.0 0.0 0.0 3.5826897329785515E-5 0.0 6 0.0 0.0 0.0 3.5826897329785515E-5 0.0 7 0.0 0.0 0.0 3.5826897329785515E-5 0.0 8 0.0 0.0 0.0 3.5826897329785515E-5 0.0 9 0.0 0.0 0.0 3.5826897329785515E-5 0.0 10-11 0.0 0.0 0.0 5.374034599467827E-5 0.0 12-13 0.0 0.0 0.0 7.165379465957103E-5 0.0 14-15 0.0 0.0 0.0 7.165379465957103E-5 0.0 16-17 0.0 0.0 0.0 7.165379465957103E-5 0.0 18-19 0.0 3.5826897329785515E-5 0.0 1.0748069198935654E-4 0.0 20-21 0.0 7.165379465957103E-5 0.0 1.0748069198935654E-4 0.0 22-23 0.0 7.165379465957103E-5 0.0 1.7913448664892757E-4 0.0 24-25 0.0 7.165379465957103E-5 0.0 3.403555246329624E-4 0.0 26-27 0.0 7.165379465957103E-5 0.0 7.34451395260603E-4 0.0 28-29 0.0 7.165379465957103E-5 0.0 0.0026870172997339138 0.0 30-31 0.0 1.0748069198935654E-4 0.0 0.009117945370430413 0.0 32-33 0.0 1.0748069198935654E-4 0.0 0.02278590670174359 0.0 34-35 0.0 1.0748069198935654E-4 0.0 0.04351176680702451 0.0 36-37 0.0 1.0748069198935654E-4 0.0 0.07580971474982615 0.0 38-39 0.0 1.4330758931914206E-4 0.0 0.12729296621272795 0.0 40-41 0.0 1.4330758931914206E-4 0.0 0.18425773296708692 0.0 42-43 0.0 1.7913448664892757E-4 0.0 0.24475144910842972 0.0 44-45 0.0 1.7913448664892757E-4 0.0 0.3193967896950378 0.0 46-47 0.0 1.9704793531382032E-4 0.0 0.40801462024026236 3.5826897329785515E-5 48-49 0.0 2.149613839787131E-4 0.0 0.5040127916354227 3.5826897329785515E-5 50-51 0.0 2.5078828130849863E-4 0.0 0.605510391770705 3.5826897329785515E-5 52-53 0.0 3.2244207596806966E-4 0.0 0.7125432475434392 3.5826897329785515E-5 54-55 0.0 3.403555246329624E-4 0.0 0.8349995826166461 3.5826897329785515E-5 56-57 0.0 3.5826897329785515E-4 0.0 0.9809941892355221 3.5826897329785515E-5 58-59 0.0 3.5826897329785515E-4 0.0 1.1397431713038015 3.5826897329785515E-5 60-61 0.0 3.5826897329785515E-4 0.0 1.3114973171027935 3.5826897329785515E-5 62-63 0.0 3.5826897329785515E-4 0.0 1.4897361313184763 3.5826897329785515E-5 64-65 0.0 3.5826897329785515E-4 0.0 1.6851360293551265 3.5826897329785515E-5 66-67 0.0 3.5826897329785515E-4 0.0 1.9153059312503338 3.5826897329785515E-5 68-69 0.0 3.5826897329785515E-4 0.0 2.168028865014641 3.5826897329785515E-5 70-71 0.0 3.5826897329785515E-4 0.0 2.4485534711068615 3.5826897329785515E-5 72-73 0.0 3.5826897329785515E-4 0.0 2.7353119573344644 3.5826897329785515E-5 74-75 0.0 3.5826897329785515E-4 0.0 3.046880569962944 3.5826897329785515E-5 76-77 0.0 3.940958706276407E-4 0.0 3.379802013399976 3.5826897329785515E-5 78-79 0.0 3.940958706276407E-4 0.0 3.7669295524969737 3.5826897329785515E-5 80-81 0.0 4.1200931929253346E-4 0.0 4.183435147404396 3.5826897329785515E-5 82-83 0.0 4.299227679574262E-4 0.0 4.605726786230576 3.5826897329785515E-5 84-85 0.0 4.657496652872117E-4 0.0 5.057181519483205 3.5826897329785515E-5 86-87 0.0 4.657496652872117E-4 0.0 5.534861541581234 3.5826897329785515E-5 88-89 0.0 5.015765626169973E-4 0.0 6.050213546221534 3.5826897329785515E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10245 0.0 23.635672 1 TACATGG 10410 0.0 22.793993 2 ACATGGG 10365 0.0 22.34542 3 GAGTACT 8105 0.0 21.817232 12-13 GTATCAA 17575 0.0 21.099176 1 CGTACAC 235 1.1681004E-7 20.154922 3 GTACTTT 8905 0.0 19.990505 14-15 CATGGGG 5335 0.0 19.797915 4 GGTATCA 13800 0.0 19.748884 1 AGTACTT 8550 0.0 19.629633 12-13 AGAGTAC 13885 0.0 19.059711 10-11 CGACGGT 175 1.0200752E-4 18.936794 7 ATCAACG 19850 0.0 18.468407 3 CAACGCA 19900 0.0 18.421675 5 TCAACGC 19950 0.0 18.399576 4 TATCAAC 20315 0.0 18.138283 2 AACGCAG 20295 0.0 18.008383 6 ACTTTTT 10890 0.0 16.564808 16-17 GAGTACA 8600 0.0 16.562756 1 ACGCAGA 22055 0.0 16.54984 7 >>END_MODULE