FastQCFastQC Report
Fri 27 May 2016
SRR939094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791199
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79730.2856478524103799No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75050.2688808644600403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65900.23609925340328652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42540.1524076212409076No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37110.13295361599083405No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30570.1095228251371543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG105550.022.483861
TACATGG106700.021.925142
ACATGGG105100.021.085023
GTATCAA183850.020.9952561
GAGTACT87400.020.93204712-13
AGTACTT92450.020.17364512-13
GTAAGCG3300.020.13157894-95
AGAGTAC146550.020.12548310-11
CATGGGG53700.019.617274
GTACTTT95900.019.101514-15
GCACCGT3506.548362E-1118.9810316
GGTATCA145950.018.3824791
TCAACGC212700.017.8923234
CAACGCA213000.017.88945
ATCAACG212700.017.8700143
TATCAAC216600.017.7235182
AACGCAG217300.017.5135616
ACCGTGC3652.3264874E-916.900928
GTGTAGC18050.016.8967251
TACTTTT111600.016.77573614-15
Produced by FastQC (version 0.11.5)