##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939094_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2791199 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48149522839468 34.0 31.0 34.0 31.0 34.0 2 32.70201551376308 34.0 31.0 34.0 31.0 34.0 3 32.80930417358275 34.0 31.0 34.0 31.0 34.0 4 36.26239476296745 37.0 37.0 37.0 35.0 37.0 5 36.222913163841056 37.0 37.0 37.0 35.0 37.0 6 36.26115945154753 37.0 37.0 37.0 35.0 37.0 7 36.21783183499277 37.0 37.0 37.0 35.0 37.0 8 36.18926776628968 37.0 37.0 37.0 35.0 37.0 9 37.93772783667521 39.0 38.0 39.0 35.0 39.0 10-11 37.850755356389854 39.0 38.0 39.0 35.0 39.0 12-13 37.77030534189787 39.0 38.0 39.0 35.0 39.0 14-15 39.18070692917273 40.5 38.5 41.0 36.0 41.0 16-17 39.2820316645284 41.0 39.0 41.0 36.0 41.0 18-19 39.31164993968542 41.0 39.0 41.0 36.0 41.0 20-21 39.28268120617699 41.0 39.0 41.0 36.0 41.0 22-23 39.21293250678293 41.0 39.0 41.0 36.0 41.0 24-25 39.137248902711704 41.0 39.0 41.0 36.0 41.0 26-27 38.99700720013156 40.0 39.0 41.0 35.0 41.0 28-29 38.84827488115323 40.0 38.0 41.0 35.0 41.0 30-31 38.667419270356575 40.0 38.0 41.0 35.0 41.0 32-33 38.5774066986983 40.0 38.0 41.0 34.5 41.0 34-35 38.47393879834436 40.0 38.0 41.0 34.0 41.0 36-37 38.324532038023804 40.0 38.0 41.0 34.0 41.0 38-39 38.19992895526259 40.0 38.0 41.0 34.0 41.0 40-41 38.027469915258635 40.0 38.0 41.0 33.0 41.0 42-43 37.84088576271344 40.0 38.0 41.0 33.0 41.0 44-45 37.591880765219535 40.0 37.0 41.0 33.0 41.0 46-47 37.29409386432139 40.0 37.0 41.0 32.0 41.0 48-49 36.97091106725102 40.0 37.0 41.0 31.0 41.0 50-51 36.71767168876171 40.0 36.0 41.0 31.0 41.0 52-53 36.46037330193941 39.0 36.0 41.0 30.0 41.0 54-55 36.21907646140601 39.0 35.5 40.0 30.0 41.0 56-57 35.9654277606147 39.0 35.0 40.0 29.5 41.0 58-59 35.62581295708404 38.5 35.0 40.0 28.5 41.0 60-61 35.24293860810354 38.0 34.5 40.0 28.0 41.0 62-63 34.84901936407974 38.0 34.0 40.0 27.0 41.0 64-65 34.38886657669339 37.0 34.0 40.0 26.0 41.0 66-67 33.95900435619245 36.5 33.5 39.0 26.0 40.5 68-69 33.44941976548429 36.0 33.0 39.0 25.5 40.0 70-71 32.92477283060076 35.5 32.0 38.5 24.0 40.0 72-73 32.32174918377371 35.0 31.5 37.5 22.0 39.5 74-75 31.76014196766336 35.0 31.0 37.0 20.5 39.0 76-77 29.730459383225632 32.5 28.5 35.0 18.0 37.0 78-79 30.735435560130252 34.0 30.5 36.0 19.0 38.0 80-81 30.742102229185377 34.0 31.0 35.5 18.5 37.0 82-83 30.3318994811907 34.0 30.0 35.0 16.5 37.0 84-85 29.831310845267573 34.0 30.0 35.0 9.0 36.0 86-87 29.110800949699396 34.0 29.0 35.0 4.5 36.0 88-89 28.408912621421834 33.5 28.5 35.0 2.0 35.0 90-91 27.822609745847572 33.0 27.0 35.0 2.0 35.0 92-93 27.334305257346394 33.0 26.0 34.5 2.0 35.0 94-95 26.748701722808015 32.0 25.5 34.0 2.0 35.0 96-97 25.88237402635928 32.0 24.5 34.0 2.0 35.0 98-99 24.58835020362217 32.0 11.0 34.0 2.0 35.0 100-101 18.62159183204064 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19491371233088017 1101 2 0.15837867680765783 1101 3 0.16599430803150028 1101 4 0.13037994414234788 1101 5 0.14994457168526765 1101 6 0.11749918414020044 1101 7 0.1403329397598938 1101 8 0.1750065415738007 1101 9 0.17227719467736335 1101 10-11 0.2168182724272114 1101 12-13 0.21064372579827761 1101 14-15 0.2589108185435123 1101 16-17 0.23822683118632426 1101 18-19 0.2541376874062493 1101 20-21 0.2010617192923334 1101 22-23 0.16770269990237807 1101 24-25 0.1799027694141273 1101 26-27 0.22353522417029836 1101 28-29 0.16426711862748533 1101 30-31 0.1874818599460113 1101 32-33 0.2847386952819022 1101 34-35 0.27741634079365696 1101 36-37 0.26188537373824516 1101 38-39 0.28993248183400055 1101 40-41 0.2872555115293096 1101 42-43 0.29564277386493387 1101 44-45 0.2887541848383961 1101 46-47 0.3265587482583001 1101 48-49 0.25838109201529136 1101 50-51 0.14622423297149112 1101 52-53 0.14143517208002976 1101 54-55 0.10884778751411517 1101 56-57 0.1256923372574832 1101 58-59 0.1367522033946642 1101 60-61 0.1140790708491366 1101 62-63 0.14094110063826548 1101 64-65 0.11113759045166205 1101 66-67 0.05959327425155436 1101 68-69 0.18299862443814163 1101 70-71 0.22562371946796844 1101 72-73 0.09708221357608693 1101 74-75 0.0366942323582613 1101 76-77 0.10248419699646405 1101 78-79 0.07928448785806097 1101 80-81 0.13595228286927963 1101 82-83 0.15396288843867367 1101 84-85 0.009707732921071965 1101 86-87 0.24179349657079996 1101 88-89 0.06580561014415665 1101 90-91 0.25778497656686383 1101 92-93 0.2554612366639084 1101 94-95 0.2295290913794723 1101 96-97 0.07460150986737446 1101 98-99 -0.027926377194454233 1101 100-101 -0.6794714359302034 1102 1 0.028630679780611956 1102 2 0.03565942947517442 1102 3 0.01914734550898345 1102 4 0.01988896902619075 1102 5 0.05796807479888599 1102 6 0.12018514490617349 1102 7 0.06678079753306321 1102 8 0.027236247416034587 1102 9 0.05617193869858994 1102 10-11 0.050821443106649156 1102 12-13 0.1634389831410914 1102 14-15 0.15709736876634395 1102 16-17 0.16692177577597533 1102 18-19 0.08510510303806029 1102 20-21 0.16054160538170947 1102 22-23 0.08739155088601791 1102 24-25 0.1605190684879787 1102 26-27 0.141654480967496 1102 28-29 0.09232510879531475 1102 30-31 0.08571416788885955 1102 32-33 0.047730495080919866 1102 34-35 0.10009969528341145 1102 36-37 0.18437005621098024 1102 38-39 0.08145482899973899 1102 40-41 0.21995286474967202 1102 42-43 0.26395911942723416 1102 44-45 0.20201555502287505 1102 46-47 0.21746381856164732 1102 48-49 0.183521636069365 1102 50-51 0.16816010117812397 1102 52-53 0.26397417227210696 1102 54-55 0.19610184411867948 1102 56-57 0.26257646966327286 1102 58-59 0.04927788339266925 1102 60-61 -0.014122055730290128 1102 62-63 -0.04897187966121663 1102 64-65 0.20215595895754745 1102 66-67 0.07323542521932325 1102 68-69 0.10461617201921314 1102 70-71 0.024812572539410382 1102 72-73 0.08771171105541242 1102 74-75 0.07212749707241528 1102 76-77 0.11986708507064492 1102 78-79 0.10845633923976905 1102 80-81 0.2487027846383434 1102 82-83 0.1300437797364289 1102 84-85 0.1454819361451989 1102 86-87 0.3958684390169829 1102 88-89 0.39600575754316836 1102 90-91 0.5564981581307116 1102 92-93 0.31025845515128125 1102 94-95 0.17398983229960763 1102 96-97 -0.12341163077550377 1102 98-99 -0.06576261814382178 1102 100-101 -0.8389866755127535 1103 1 0.07594780801661472 1103 2 0.10779486853942188 1103 3 0.1621388738481926 1103 4 0.06490273009955416 1103 5 0.11680472655772434 1103 6 0.14536629653600386 1103 7 0.13202226136724704 1103 8 0.1286623461517422 1103 9 0.18227658736118002 1103 10-11 0.2034242211233277 1103 12-13 0.20532425019192146 1103 14-15 0.06383787577588862 1103 16-17 0.20126532957317522 1103 18-19 0.269652653997035 1103 20-21 0.22585429352137965 1103 22-23 0.25738112257260326 1103 24-25 0.3024771724700912 1103 26-27 0.18582430121347215 1103 28-29 0.2388765085544975 1103 30-31 0.28873434721284497 1103 32-33 0.2946544113963938 1103 34-35 0.20867241395546898 1103 36-37 0.15907467692139932 1103 38-39 0.22125738984948384 1103 40-41 0.2926930384832218 1103 42-43 0.2457068832528151 1103 44-45 0.323150628558615 1103 46-47 0.41626966916799546 1103 48-49 0.44886308270220354 1103 50-51 0.5257625566696476 1103 52-53 0.47083374481876206 1103 54-55 0.4373950087355212 1103 56-57 0.34438228801710125 1103 58-59 0.29460664403598713 1103 60-61 0.47873132433335996 1103 62-63 0.393962669233602 1103 64-65 0.5040663829706205 1103 66-67 0.3479516030811851 1103 68-69 0.35124712798377544 1103 70-71 0.3071591580730839 1103 72-73 0.3804126979823934 1103 74-75 0.3684232956446998 1103 76-77 0.35134070251280747 1103 78-79 0.36268141879597593 1103 80-81 0.5561539220770655 1103 82-83 0.4416622109740622 1103 84-85 0.37846201484133957 1103 86-87 0.3468284889008757 1103 88-89 0.37086223384832095 1103 90-91 0.5843495492892217 1103 92-93 0.6939007855030503 1103 94-95 0.49346443881891844 1103 96-97 0.16866983907914346 1103 98-99 0.16589107584430707 1103 100-101 -0.35962291013905556 1104 1 0.10434896659078419 1104 2 0.07445494111543383 1104 3 0.08691702748554064 1104 4 0.033568712103196674 1104 5 0.03539452576927715 1104 6 0.022485525033872023 1104 7 0.10945881563866067 1104 8 0.04158910577026376 1104 9 0.08268677001857583 1104 10-11 0.17810841706300806 1104 12-13 0.12501891033831214 1104 14-15 0.08281355879849173 1104 16-17 0.1388516139509477 1104 18-19 0.20639042136528474 1104 20-21 0.255560321189364 1104 22-23 0.16770202922877075 1104 24-25 0.16578693435758396 1104 26-27 0.21186567109209165 1104 28-29 0.11489807997270418 1104 30-31 0.15448823956102586 1104 32-33 0.18838888668376796 1104 34-35 0.22018935483269786 1104 36-37 0.24381751062339418 1104 38-39 0.24881197402677202 1104 40-41 0.43550841516876915 1104 42-43 0.38134220176358014 1104 44-45 0.3225272117879072 1104 46-47 0.4278708708681691 1104 48-49 0.425667964099226 1104 50-51 0.44372230185067707 1104 52-53 0.31071729814218685 1104 54-55 0.23195328128666404 1104 56-57 0.33020234898923917 1104 58-59 0.43343221814277655 1104 60-61 0.34834267877469216 1104 62-63 0.3487192210184773 1104 64-65 0.32064554573047843 1104 66-67 0.34219450865985834 1104 68-69 0.24083542037546835 1104 70-71 0.2998165679747089 1104 72-73 0.16357916628672342 1104 74-75 0.05340599344151897 1104 76-77 -0.037592524710710506 1104 78-79 0.38024004684522694 1104 80-81 0.506949891414866 1104 82-83 0.44168228463793824 1104 84-85 0.15279250958592172 1104 86-87 0.3349312816007952 1104 88-89 0.45101604991657496 1104 90-91 0.551923054513555 1104 92-93 0.4619364058958162 1104 94-95 0.4575780700022527 1104 96-97 -0.09506038512425619 1104 98-99 -0.2200778863861217 1104 100-101 -0.6524850390352874 1105 1 0.014087012562065127 1105 2 0.056059884773191015 1105 3 0.039066633588454636 1105 4 0.03385057912553435 1105 5 0.025905781752527446 1105 6 0.06516134523729988 1105 7 0.09735302435425552 1105 8 0.06424473251537677 1105 9 0.16522098671183016 1105 10-11 0.10181352712982061 1105 12-13 0.08262487925036055 1105 14-15 0.04562308275640703 1105 16-17 0.12819816236538628 1105 18-19 0.15802521893209587 1105 20-21 0.18741324238501988 1105 22-23 0.1309708141310466 1105 24-25 0.1255012187363178 1105 26-27 0.14204639942775543 1105 28-29 0.10924227559995359 1105 30-31 0.1639271231868733 1105 32-33 0.19841861594184707 1105 34-35 0.2202440454489718 1105 36-37 0.26191558601230014 1105 38-39 0.14294722345337618 1105 40-41 0.23096667890530398 1105 42-43 0.17863859997359555 1105 44-45 0.26233995485731043 1105 46-47 0.12569539968746568 1105 48-49 0.027820063221689395 1105 50-51 0.07534656640991244 1105 52-53 0.15112493800058502 1105 54-55 0.017054606949805873 1105 56-57 0.08394702015996813 1105 58-59 0.011961554750278935 1105 60-61 -0.017179795796515407 1105 62-63 0.07961100527256093 1105 64-65 0.19599262079529467 1105 66-67 0.028197138795476917 1105 68-69 -0.1568697551331084 1105 70-71 -0.001122599232111554 1105 72-73 -0.18176262669292953 1105 74-75 -0.12270900955010333 1105 76-77 -0.19827628206511605 1105 78-79 -0.18617968104168625 1105 80-81 -0.07290705600734881 1105 82-83 0.07085762493510472 1105 84-85 -0.031006836619702938 1105 86-87 -0.03850785444328153 1105 88-89 0.2001616453742976 1105 90-91 0.1689503001009136 1105 92-93 0.05337724654200926 1105 94-95 0.023035258624112487 1105 96-97 -0.1753388865702057 1105 98-99 -0.3052610142530945 1105 100-101 -0.9718159904961965 1106 1 0.04172692269278855 1106 2 0.04152430951367592 1106 3 0.01001831082798077 1106 4 0.02246868874683372 1106 5 0.08562650214852852 1106 6 0.12747061330727405 1106 7 0.09449985490019941 1106 8 0.07693726663686817 1106 9 0.10881629861110298 1106 10-11 0.1077102225114075 1106 12-13 0.0917356329268344 1106 14-15 -0.008250953071154754 1106 16-17 0.035367129829822375 1106 18-19 0.08255124257685509 1106 20-21 0.14843597102304784 1106 22-23 0.13302741495764536 1106 24-25 0.17779353118826435 1106 26-27 0.24066584567439264 1106 28-29 0.18911472549902442 1106 30-31 0.12222831743907392 1106 32-33 0.23949806855623024 1106 34-35 0.13251741987227206 1106 36-37 0.16320715656209472 1106 38-39 0.2979709072528749 1106 40-41 0.14418778076516503 1106 42-43 0.2382470588595993 1106 44-45 0.15981793808492029 1106 46-47 0.13876939766901586 1106 48-49 0.14259382811980714 1106 50-51 0.14045043271133295 1106 52-53 0.22095279867917128 1106 54-55 0.18298650513800396 1106 56-57 0.011225741084608387 1106 58-59 -0.0729854908571852 1106 60-61 -0.05235827282457706 1106 62-63 -0.1299080198620075 1106 64-65 -0.09497457030909118 1106 66-67 0.05296375500078199 1106 68-69 -0.2759525598236152 1106 70-71 -0.33875858959640226 1106 72-73 -0.17159706537036357 1106 74-75 -0.18758850301415464 1106 76-77 -0.281028934290223 1106 78-79 -0.06532329467586706 1106 80-81 0.07287318776908336 1106 82-83 -0.05373853399415296 1106 84-85 -0.23916468155914572 1106 86-87 -0.1474912532562378 1106 88-89 -0.18925731307918525 1106 90-91 0.13481161245673334 1106 92-93 0.1339081721475459 1106 94-95 -0.09538328832898202 1106 96-97 -0.07664742775628497 1106 98-99 -0.06751659203567684 1106 100-101 -1.043882398248023 1107 1 -0.0016955282753343681 1107 2 0.011784455276384165 1107 3 0.079877631523118 1107 4 0.0035597593264355964 1107 5 -0.07803567290388003 1107 6 0.005828297452843856 1107 7 0.025735898462407647 1107 8 0.07378255405123468 1107 9 0.005690792851524407 1107 10-11 0.024216670318892852 1107 12-13 0.04584532787503548 1107 14-15 0.011377679897790927 1107 16-17 0.04272961772588246 1107 18-19 0.10244234717092837 1107 20-21 0.11430087205550876 1107 22-23 0.042138024514066785 1107 24-25 0.0338318872727541 1107 26-27 0.020918984975075716 1107 28-29 0.029679238728945734 1107 30-31 -0.01090536132308273 1107 32-33 0.016937145325996994 1107 34-35 -0.1770726263028095 1107 36-37 -0.0897214305104157 1107 38-39 -0.1139626438250616 1107 40-41 -0.018067760049824244 1107 42-43 0.0024553568033027773 1107 44-45 0.012585360649651989 1107 46-47 0.08806191536528019 1107 48-49 0.04238951027388538 1107 50-51 0.006412035335934263 1107 52-53 0.055080621205611635 1107 54-55 -0.0693768583853469 1107 56-57 -0.026425005046569083 1107 58-59 -0.041815758195646424 1107 60-61 -0.15532785484645473 1107 62-63 -0.04257628936694147 1107 64-65 -0.10370131872311106 1107 66-67 -0.20602384083276348 1107 68-69 -0.19423949790441952 1107 70-71 -0.3283731302356827 1107 72-73 -0.44330623352733056 1107 74-75 -0.3517639992234045 1107 76-77 -0.2857320562155117 1107 78-79 -0.16972759164533002 1107 80-81 -0.07353934630494763 1107 82-83 -0.24454573241562727 1107 84-85 -0.24335318296311215 1107 86-87 -0.38754552049191204 1107 88-89 -0.35739774781477607 1107 90-91 -0.16902224906825936 1107 92-93 0.02687107399225397 1107 94-95 -0.0029503250690900984 1107 96-97 -0.5092302626028768 1107 98-99 -0.4323738070384877 1107 100-101 -0.9562211344832434 1108 1 -0.04068347736467359 1108 2 0.008850063344617354 1108 3 0.04477290648222976 1108 4 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-1.200254213207728 2316 98-99 -0.8723745199254509 2316 100-101 0.35484370498326356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 20.0 5 56.0 6 100.0 7 259.0 8 587.0 9 1168.0 10 2080.0 11 3328.0 12 5194.0 13 7277.0 14 9034.0 15 12151.0 16 16023.0 17 22751.0 18 22419.0 19 16171.0 20 14772.0 21 15563.0 22 16985.0 23 19064.0 24 21719.0 25 25307.0 26 29778.0 27 35424.0 28 43090.0 29 52536.0 30 64596.0 31 80222.0 32 100389.0 33 131244.0 34 183560.0 35 271967.0 36 444511.0 37 687308.0 38 418925.0 39 15613.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.116922948498 19.703392879759587 15.054878470023372 23.124805701719037 2 15.273579562044842 20.92459190476924 40.13726717442934 23.66456135875658 3 19.28203614289056 24.705404379981506 30.14353329877232 25.869026178355607 4 13.011827533615483 17.308224888300693 37.29275483403369 32.38719274405014 5 13.7888771098012 37.47382397313842 34.2813608058759 14.455938111184476 6 31.555865418409795 36.33657077119904 17.660904865615098 14.446658944776061 7 28.022580976849014 32.90213273937114 21.085239712395996 17.99004657138384 8 25.567901106298763 35.39192296930459 20.128374938512085 18.91180098588456 9 25.854516284937045 17.5353315904742 21.01351426394177 35.596637860646986 10-11 24.672479461335435 26.81874348622223 27.477241859143685 21.03153519329865 12-13 25.793395598092435 24.219376690805635 27.835546659338874 22.15168105176306 14-15 22.569746176743877 25.63487162841306 26.5561683800135 25.239213814829565 16-17 22.03804276268353 28.749975144640278 27.080608573854096 22.13137351882209 18-19 21.794146529860463 27.579473910674228 29.293163977201196 21.33321558226411 20-21 22.701247743353303 26.552191370088625 29.152883760706423 21.593677125851652 22-23 22.265897200450414 26.614942180761748 29.017547656043156 22.101612962744685 24-25 22.322830139096162 26.65546717655516 29.156185058139005 21.865517626209673 26-27 22.079274929580293 26.871397953941102 29.179018330262885 21.870308786215723 28-29 21.778286918070112 27.38795803593261 29.104371869063367 21.729383176933915 30-31 22.152845425926277 26.761993680851848 28.93555780150394 22.149603091717932 32-33 21.808091006084126 26.862784367142712 29.0122556694294 22.316868957343765 34-35 22.626204609310744 26.8139659166622 28.74847114428725 21.811358329739804 36-37 22.363576159652364 26.63304202488933 28.839786806225238 22.16359500923307 38-39 22.017881797811913 27.13805233817532 28.985985030775886 21.858080833236883 40-41 22.393866247125118 26.8255843908759 28.851322276058294 21.92922708594069 42-43 22.120262632234255 26.89819865817501 28.446564372665467 22.534974336925266 44-45 22.332791672406625 26.912856961925314 28.37930046739528 22.375050898272782 46-47 22.47374662763743 27.215566073226398 28.03552042764961 22.27516687148656 48-49 22.543725169080446 27.2562481033647 27.91135981988638 22.288666907668482 50-51 22.16034088753359 27.413289949123747 28.12680422953051 22.29956493381215 52-53 22.56893901151441 27.47604165808314 27.620065785348878 22.334953545053576 54-55 22.125724464647632 27.179717390268483 27.926385757518542 22.768172387565343 56-57 22.393946543035188 27.474846378715096 27.726082541245272 22.405124537004443 58-59 22.713013433114952 27.100791713272105 27.74541570858302 22.440779145029918 60-61 22.464164687036984 27.159366802734297 28.204245833193752 22.172222677034963 62-63 22.22364536149549 27.265744209684183 28.123789709616293 22.38682071920403 64-65 22.02132462004772 27.2873449558245 27.935234348689065 22.75609607543871 66-67 22.07423391869226 27.34411233560236 27.642521245378006 22.939132500327375 68-69 22.096441308998095 27.191463999901117 27.849628273332723 22.862466417768072 70-71 22.259370753950588 27.039989108617355 27.78850296333384 22.912137174098216 72-73 22.32356059170271 26.852259548674244 27.950318124934842 22.873861734688212 74-75 22.102974384843215 27.419453073750745 27.523171941520474 22.95440059988557 76-77 22.113929277843752 26.968087042543498 27.706447483580188 23.211536196032565 78-79 22.060486856794356 27.150143232696294 27.66000827379563 23.12936163671372 80-81 22.171606641823036 27.077378961636995 27.65985422354575 23.091160172994222 82-83 22.266147988731724 27.055021874112168 27.71832105127581 22.960509085880297 84-85 22.013299660826764 27.05720731484928 27.63040184522852 23.299091179095434 86-87 22.050366201507128 26.985290610242597 27.73164518282101 23.232698005429274 88-89 21.970806898029206 26.94498812536122 27.486366605166417 23.59783837144316 90-91 21.953816546300565 26.8302357635737 27.626530121028107 23.589417569097627 92-93 22.179214022361 26.80582788973484 27.659779901038945 23.355178186865214 94-95 21.905464623888268 26.529392302267286 27.77787033061246 23.787272743231984 96-97 21.777803254696238 26.389372303464732 27.997266406264693 23.835558035574337 98-99 21.67141910800277 26.508680684308562 27.507248682196206 24.31265152549247 100-101 21.949383596243894 25.373048771312597 28.42067361949563 24.256894012947882 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 321.0 1 223.0 2 174.5 3 233.0 4 207.5 5 209.0 6 393.0 7 872.5 8 1424.0 9 1742.5 10 1777.5 11 1754.5 12 1955.0 13 2180.0 14 2371.5 15 2593.0 16 2782.0 17 3054.5 18 3404.0 19 3675.0 20 4077.0 21 4801.5 22 5855.5 23 7338.5 24 8834.0 25 11089.0 26 14687.5 27 18632.0 28 22246.5 29 26456.0 30 31405.5 31 36904.5 32 43181.0 33 48653.0 34 53708.0 35 58845.0 36 64780.5 37 71448.5 38 75858.5 39 78021.5 40 80315.5 41 85626.5 42 92122.0 43 97484.0 44 102378.0 45 107150.0 46 109135.5 47 115276.0 48 129928.5 49 130729.5 50 119179.0 51 111070.5 52 102675.0 53 96756.0 54 92322.0 55 84572.5 56 75172.0 57 65440.5 58 56592.5 59 48229.0 60 39579.5 61 31453.5 62 24873.0 63 19035.5 64 13957.0 65 10361.5 66 7661.5 67 5857.5 68 4330.0 69 2976.0 70 2280.5 71 1864.0 72 1588.0 73 1438.5 74 1191.0 75 905.0 76 619.5 77 383.5 78 257.0 79 164.5 80 102.5 81 66.0 82 36.0 83 19.0 84 10.5 85 8.0 86 6.0 87 3.0 88 3.0 89 2.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42874048034554324 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.777589845797452E-4 16-17 0.0018092583151541686 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.7913448664892757E-5 26-27 0.0027586710943934846 28-29 5.374034599467827E-5 30-31 0.0 32-33 3.5826897329785515E-5 34-35 0.010909290236919689 36-37 0.024577251568232865 38-39 0.019346524558084176 40-41 0.007738609823233672 42-43 0.008025225001871956 44-45 0.00331398800300516 46-47 0.004621669755542331 48-49 0.0025795366077445573 50-51 5.1949001128189E-4 52-53 0.0 54-55 0.0 56-57 1.7913448664892757E-5 58-59 0.001325595201202064 60-61 0.0022391810831115947 62-63 0.0010748069198935654 64-65 0.0021137869424573455 66-67 0.0022391810831115947 68-69 0.003672256976303015 70-71 5.374034599467827E-5 72-73 0.0 74-75 0.0 76-77 0.09757455487767085 78-79 0.01649828622036623 80-81 2.6870172997339135E-4 82-83 0.0 84-85 0.0 86-87 0.10676415404276082 88-89 0.41328117414774085 90-91 0.4213959663929372 92-93 0.0 94-95 1.7913448664892757E-5 96-97 5.374034599467827E-5 98-99 7.165379465957103E-5 100-101 3.2244207596806966E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2791199.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.8407261955878 #Duplication Level Percentage of deduplicated Percentage of total 1 70.92626962528155 35.35016784468093 2 15.493968387116103 15.444612721306934 3 5.816191723643884 8.69649657597536 4 2.7059832180494876 5.394726746426402 5 1.4887124018270965 3.70992536017201 6 0.8739794755564585 2.613586304506372 7 0.570702625870452 1.9910963320578525 8 0.39300943634525676 1.5670300567332989 9 0.2657699476895357 1.1921550474428857 >10 1.306795988112687 11.71311650519169 >50 0.09617303432437782 3.3148689376225087 >100 0.056610509798519386 5.417192553209076 >500 0.004249184650539451 1.4948251346673274 >1k 0.0013683814976056237 1.3080617311414326 >5k 2.1606023646404584E-4 0.7921381488660886 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7973 0.2856478524103799 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7505 0.2688808644600403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6590 0.23609925340328652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4254 0.1524076212409076 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3711 0.13295361599083405 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3057 0.1095228251371543 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.5826897329785515E-5 0.0 5 0.0 0.0 0.0 3.5826897329785515E-5 3.5826897329785515E-5 6 0.0 0.0 0.0 3.5826897329785515E-5 3.5826897329785515E-5 7 0.0 0.0 0.0 3.5826897329785515E-5 3.5826897329785515E-5 8 0.0 0.0 0.0 3.5826897329785515E-5 3.5826897329785515E-5 9 0.0 0.0 0.0 3.5826897329785515E-5 3.5826897329785515E-5 10-11 0.0 0.0 0.0 5.374034599467827E-5 3.5826897329785515E-5 12-13 0.0 7.165379465957103E-5 0.0 7.165379465957103E-5 3.5826897329785515E-5 14-15 0.0 7.165379465957103E-5 0.0 7.165379465957103E-5 3.5826897329785515E-5 16-17 0.0 8.956724332446379E-5 0.0 7.165379465957103E-5 3.5826897329785515E-5 18-19 0.0 1.4330758931914206E-4 0.0 7.165379465957103E-5 3.5826897329785515E-5 20-21 0.0 1.7913448664892757E-4 0.0 7.165379465957103E-5 3.5826897329785515E-5 22-23 0.0 1.9704793531382032E-4 0.0 1.4330758931914206E-4 3.5826897329785515E-5 24-25 0.0 2.149613839787131E-4 0.0 3.045286273031769E-4 3.5826897329785515E-5 26-27 0.0 2.149613839787131E-4 0.0 7.165379465957103E-4 3.5826897329785515E-5 28-29 0.0 2.149613839787131E-4 0.0 0.0026870172997339138 3.5826897329785515E-5 30-31 0.0 2.149613839787131E-4 0.0 0.009243339511084664 3.5826897329785515E-5 32-33 0.0 2.149613839787131E-4 0.0 0.022911300842397838 3.5826897329785515E-5 34-35 0.0 2.149613839787131E-4 0.0 0.04404917026697129 3.5826897329785515E-5 36-37 0.0 2.149613839787131E-4 0.0 0.07654416614508676 3.5826897329785515E-5 38-39 0.0 2.149613839787131E-4 0.0 0.12851108072194065 3.5826897329785515E-5 40-41 0.0 2.149613839787131E-4 0.0 0.18579828955226768 3.5826897329785515E-5 42-43 0.0 2.5078828130849863E-4 0.0 0.24663236121824347 3.5826897329785515E-5 44-45 0.0 2.5078828130849863E-4 0.0 0.32210172044343666 3.5826897329785515E-5 46-47 0.0 2.687017299733914E-4 0.0 0.4114540023839217 3.5826897329785515E-5 48-49 0.0 3.045286273031769E-4 0.0 0.5079358368930341 3.5826897329785515E-5 50-51 0.0 3.2244207596806966E-4 0.0 0.6101320615262473 3.5826897329785515E-5 52-53 0.0 4.657496652872117E-4 0.0 0.7178635417969124 3.5826897329785515E-5 54-55 0.0 4.836631139521045E-4 0.0 0.8409289341247257 3.5826897329785515E-5 56-57 0.0 5.015765626169973E-4 0.0 0.9877654728308516 3.5826897329785515E-5 58-59 0.0 5.015765626169973E-4 0.0 1.1471951659483972 3.5826897329785515E-5 60-61 0.0 5.015765626169973E-4 0.0 1.320131599359272 3.5826897329785515E-5 62-63 0.0 5.015765626169973E-4 0.0 1.499176518764875 3.5826897329785515E-5 64-65 0.0 5.015765626169973E-4 0.0 1.6959557523487219 3.5826897329785515E-5 66-67 0.0 5.015765626169973E-4 0.0 1.927433335996466 3.5826897329785515E-5 68-69 0.0 5.015765626169973E-4 0.0 2.181804307037943 3.5826897329785515E-5 70-71 0.0 5.1949001128189E-4 0.0 2.4637261621260254 3.5826897329785515E-5 72-73 0.0 5.374034599467827E-4 0.0 2.7510220518135755 3.5826897329785515E-5 74-75 0.0 5.374034599467827E-4 0.0 3.0639699999892516 3.5826897329785515E-5 76-77 0.0 5.374034599467827E-4 0.0 3.3989335765740813 3.5826897329785515E-5 78-79 0.0 5.374034599467827E-4 0.0 3.7866343460283556 3.5826897329785515E-5 80-81 0.0 5.553169086116754E-4 0.0 4.2031578543844414 3.5826897329785515E-5 82-83 0.0 6.269707032712465E-4 0.0 4.6242492921500755 3.5826897329785515E-5 84-85 0.0 6.807110492659247E-4 0.0 5.0752561891860815 3.5826897329785515E-5 86-87 0.0 6.807110492659247E-4 0.0 5.551771837120893 3.5826897329785515E-5 88-89 0.0 6.807110492659247E-4 0.0 6.065081708613395 3.5826897329785515E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10555 0.0 22.48386 1 TACATGG 10670 0.0 21.92514 2 ACATGGG 10510 0.0 21.08502 3 GTATCAA 18385 0.0 20.995256 1 GAGTACT 8740 0.0 20.932047 12-13 AGTACTT 9245 0.0 20.173645 12-13 GTAAGCG 330 0.0 20.131578 94-95 AGAGTAC 14655 0.0 20.125483 10-11 CATGGGG 5370 0.0 19.61727 4 GTACTTT 9590 0.0 19.1015 14-15 GCACCGT 350 6.548362E-11 18.981031 6 GGTATCA 14595 0.0 18.382479 1 TCAACGC 21270 0.0 17.892323 4 CAACGCA 21300 0.0 17.8894 5 ATCAACG 21270 0.0 17.870014 3 TATCAAC 21660 0.0 17.723518 2 AACGCAG 21730 0.0 17.513561 6 ACCGTGC 365 2.3264874E-9 16.90092 8 GTGTAGC 1805 0.0 16.896725 1 TACTTTT 11160 0.0 16.775736 14-15 >>END_MODULE