FastQCFastQC Report
Fri 27 May 2016
SRR939093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382343
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100050.41996471540831865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75660.3175865104227225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.21323545769857655No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25930.10884242949063169No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25230.10590414562470643No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24830.10422512627274913No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23910.10036338176324736No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.038.5639271
GTATCAA112300.036.05781
ATCAACG139700.028.6249313
TATCAAC140900.028.4483992
TCAACGC142100.028.1081284
CAACGCA143350.027.8299755
AACGCAG146300.027.2902986
TAAGGTA6900.025.4068644
GTACATG111700.024.3518181
TACATGG111150.024.1271152
ACGCAGA165100.024.039317
CGCAGAG165450.023.9025768
GAGTACT88400.022.90435212-13
GGACCGA6250.022.73356
ACATGGG114350.022.2917693
GCAGAGT177750.021.8488859
AGTACTT91900.021.28475812-13
GTACTTT93900.021.2608114-15
CATGGGG57800.020.3292924
TAAGGTG12800.019.6184335
Produced by FastQC (version 0.11.5)