##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939093_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2382343 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14472139402261 33.0 31.0 34.0 30.0 34.0 2 32.23718876752844 34.0 31.0 34.0 30.0 34.0 3 32.295747085956975 34.0 31.0 34.0 30.0 34.0 4 35.742539172570865 37.0 35.0 37.0 35.0 37.0 5 35.78634142942473 37.0 35.0 37.0 35.0 37.0 6 35.87505829345312 37.0 37.0 37.0 35.0 37.0 7 35.80032052479429 37.0 36.0 37.0 35.0 37.0 8 35.78886751403975 37.0 36.0 37.0 35.0 37.0 9 37.48054583240113 39.0 38.0 39.0 35.0 39.0 10-11 37.39720225005384 39.0 38.0 39.0 35.0 39.0 12-13 37.30977487288774 39.0 37.0 39.0 34.0 39.0 14-15 38.626587775144046 40.0 38.0 41.0 34.0 41.0 16-17 38.56365582118109 40.0 38.0 41.0 34.0 41.0 18-19 38.508166330373086 40.0 38.0 41.0 34.0 41.0 20-21 38.40626979406408 40.0 38.0 41.0 34.0 41.0 22-23 38.32543634564796 40.0 38.0 41.0 34.0 41.0 24-25 38.35180576432529 40.0 38.0 41.0 34.0 41.0 26-27 38.262737355620075 40.0 38.0 41.0 34.0 41.0 28-29 38.133516878132156 40.0 38.0 41.0 33.5 41.0 30-31 37.95032831124654 40.0 38.0 41.0 33.0 41.0 32-33 37.72118330567848 40.0 37.5 41.0 33.0 41.0 34-35 37.40639656002516 40.0 37.0 41.0 32.0 41.0 36-37 37.138954592180895 40.0 37.0 41.0 31.0 41.0 38-39 36.88032558703763 40.0 36.5 41.0 30.5 41.0 40-41 36.70619889747194 40.0 36.0 41.0 30.0 41.0 42-43 36.480990982406816 40.0 36.0 41.0 30.0 41.0 44-45 36.19461744173698 39.0 35.0 41.0 29.5 41.0 46-47 35.80250304007441 39.0 35.0 41.0 27.5 41.0 48-49 35.735823514917875 39.0 35.0 41.0 27.5 41.0 50-51 34.701188703725705 38.0 34.0 40.0 25.5 40.5 52-53 34.8335613301695 38.5 34.5 39.5 26.0 40.5 54-55 35.305020099960416 39.0 35.0 41.0 27.0 41.0 56-57 35.1527110076089 39.0 35.0 41.0 26.0 41.0 58-59 34.92748504308574 38.5 35.0 41.0 26.0 41.0 60-61 34.61778656557851 38.0 35.0 40.0 26.0 41.0 62-63 34.23114114970011 37.0 34.0 40.0 24.5 41.0 64-65 33.84151001765909 37.0 34.0 40.0 23.5 41.0 66-67 33.44684371645897 36.0 34.0 39.0 22.5 41.0 68-69 33.039827178538104 35.5 33.0 39.0 22.0 41.0 70-71 32.63300288833304 35.0 33.0 38.5 20.5 40.5 72-73 32.204037369933715 35.0 33.0 37.5 19.5 40.0 74-75 31.773092707473275 35.0 32.0 37.0 18.0 39.0 76-77 31.36446871840033 35.0 32.0 36.5 14.5 39.0 78-79 30.96699047954052 35.0 31.5 36.0 9.5 38.5 80-81 30.584994268247687 35.0 31.0 35.5 7.0 37.0 82-83 30.18684484140193 35.0 31.0 35.0 3.5 37.0 84-85 29.836118056887692 34.0 30.5 35.0 2.0 36.5 86-87 29.48513144412874 34.0 30.0 35.0 2.0 36.0 88-89 29.100025479118667 34.0 30.0 35.0 2.0 36.0 90-91 28.742865531957406 34.0 29.0 35.0 2.0 35.0 92-93 28.35452997322384 34.0 29.0 35.0 2.0 35.0 94-95 27.918569240449422 34.0 29.0 35.0 2.0 35.0 96-97 27.379850004806194 34.0 27.0 35.0 2.0 35.0 98-99 26.6960441884313 34.0 25.5 35.0 2.0 35.0 100-101 24.158224067651048 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15230996922463902 1101 2 0.18173367421096742 1101 3 0.0742486479109914 1101 4 -0.04595038942880336 1101 5 0.13643858662094743 1101 6 0.22818433258934334 1101 7 0.03719690303431378 1101 8 0.20973354534955746 1101 9 0.22273569690852923 1101 10-11 0.2652634634685924 1101 12-13 0.17186296197851902 1101 14-15 0.2404372328394686 1101 16-17 0.2657358877938094 1101 18-19 0.29086068511685426 1101 20-21 0.30564135607625786 1101 22-23 0.2611676682224413 1101 24-25 0.36139411100776186 1101 26-27 0.25487343101561777 1101 28-29 0.1806368227900279 1101 30-31 0.26449384607328597 1101 32-33 0.31839869359244943 1101 34-35 0.21585906037284275 1101 36-37 0.15602818616577707 1101 38-39 0.18893422692705997 1101 40-41 0.13609506273696326 1101 42-43 0.06334636019562367 1101 44-45 0.0949870483644446 1101 46-47 0.02231068369462008 1101 48-49 0.22809088862192795 1101 50-51 0.07798571576878999 1101 52-53 0.12760243287567619 1101 54-55 0.15659310659109593 1101 56-57 0.08779031235018664 1101 58-59 0.14416601725101685 1101 60-61 0.10993427143816348 1101 62-63 0.10928002409205817 1101 64-65 0.10146288196251874 1101 66-67 0.05287967902906843 1101 68-69 -0.03969383116444902 1101 70-71 -0.007277367519016309 1101 72-73 -0.02705778754921795 1101 74-75 -0.19369145723726078 1101 76-77 -0.22180726724922906 1101 78-79 -0.2537669564467038 1101 80-81 -0.2431135387900234 1101 82-83 -0.2715571665257137 1101 84-85 -0.33646956529464234 1101 86-87 -0.4072662229213755 1101 88-89 -0.39687370985392434 1101 90-91 -0.5308567497508605 1101 92-93 -0.5751240444100212 1101 94-95 -0.6831258279078121 1101 96-97 -0.6999965974541382 1101 98-99 -0.8227243244222997 1101 100-101 -1.1224019041943798 1102 1 0.06908461743214644 1102 2 0.18756751903580948 1102 3 0.34212811777845786 1102 4 0.20609273498865832 1102 5 0.2610578096085163 1102 6 0.21796586888250857 1102 7 0.2421614964553953 1102 8 0.22845129296828048 1102 9 0.20373520814997903 1102 10-11 0.239060179693098 1102 12-13 0.24375096238771476 1102 14-15 0.258414738579539 1102 16-17 0.3564695086990355 1102 18-19 0.43892895218362327 1102 20-21 0.4550082432525997 1102 22-23 0.43094874985648346 1102 24-25 0.38791494576191354 1102 26-27 0.38054547402636985 1102 28-29 0.33438832884835534 1102 30-31 0.3342975185823178 1102 32-33 0.3797549417454036 1102 34-35 0.3463725125995367 1102 36-37 0.25660872902876264 1102 38-39 0.3266526053853127 1102 40-41 0.09366400042593881 1102 42-43 0.07943560978075226 1102 44-45 0.016811254415955545 1102 46-47 -0.05708203380293497 1102 48-49 -0.03344963855531802 1102 50-51 -0.06366049123750628 1102 52-53 -0.1375078787931514 1102 54-55 0.007947280361811693 1102 56-57 -0.07790167882942711 1102 58-59 -0.17695119386993952 1102 60-61 -0.23824581335576056 1102 62-63 -0.28283647094078646 1102 64-65 -0.22169319114666308 1102 66-67 -0.24350194478597587 1102 68-69 -0.18953100636733922 1102 70-71 -0.29929781581291337 1102 72-73 -0.31707702531320336 1102 74-75 -0.2751748962788376 1102 76-77 -0.21226724019701138 1102 78-79 -0.2738039259163436 1102 80-81 -0.23182875736739916 1102 82-83 -0.2556952465002489 1102 84-85 -0.3089221954976473 1102 86-87 -0.45205298779440994 1102 88-89 -0.3589849763069033 1102 90-91 -0.4202914663845654 1102 92-93 -0.40089298664558015 1102 94-95 -0.4415682794297844 1102 96-97 -0.49340375606197284 1102 98-99 -0.6966970789745766 1102 100-101 -0.7196520690083155 1103 1 0.41202570691262963 1103 2 0.34217426604303824 1103 3 0.39444388463643776 1103 4 0.32333154213876725 1103 5 0.2730572738807737 1103 6 0.2888795730026814 1103 7 0.32814765868231177 1103 8 0.3550113907385892 1103 9 0.3674986211077069 1103 10-11 0.4193297735166084 1103 12-13 0.3987064904748223 1103 14-15 0.5399743108530188 1103 16-17 0.39084537193221536 1103 18-19 0.4930223399454121 1103 20-21 0.4706171266601942 1103 22-23 0.5128290612110362 1103 24-25 0.4650799694371628 1103 26-27 0.5781964567054345 1103 28-29 0.46447041796331945 1103 30-31 0.5274947993920094 1103 32-33 0.45386952609601394 1103 34-35 0.3669543166440974 1103 36-37 0.37497479662904 1103 38-39 0.5011149942287787 1103 40-41 0.2561376209502626 1103 42-43 0.22531097123420807 1103 44-45 0.26945082962850364 1103 46-47 0.3734742580682351 1103 48-49 0.44011787104545874 1103 50-51 0.47852248768774786 1103 52-53 0.5642182620232532 1103 54-55 0.38243399087257757 1103 56-57 0.39673472852083336 1103 58-59 0.3119750068438023 1103 60-61 0.49941268439297204 1103 62-63 0.3408392072988917 1103 64-65 0.21222349016881026 1103 66-67 0.12360804588529106 1103 68-69 0.15418344702759867 1103 70-71 0.2546690806772389 1103 72-73 0.21166765112491248 1103 74-75 0.12442288937984003 1103 76-77 0.0022989272941735805 1103 78-79 0.04784617132382252 1103 80-81 0.11301900201643988 1103 82-83 -0.001714491775199889 1103 84-85 0.10084534888823171 1103 86-87 -0.050910334472572316 1103 88-89 0.10598670731982551 1103 90-91 -0.18875477414991693 1103 92-93 -0.2575988377039664 1103 94-95 -0.3541432789879977 1103 96-97 -0.42943893365664465 1103 98-99 -0.7247321021754907 1103 100-101 -0.718368554634349 1104 1 0.31276147304329527 1104 2 0.3194004268510682 1104 3 0.36521857153792325 1104 4 0.26853864179388154 1104 5 0.3474908298111359 1104 6 0.3619052628218995 1104 7 0.3022986740870621 1104 8 0.3776414534726129 1104 9 0.38497383615683844 1104 10-11 0.39242488933800246 1104 12-13 0.3883785482456972 1104 14-15 0.5426759452440066 1104 16-17 0.548825361063237 1104 18-19 0.5865895965498922 1104 20-21 0.5449752768947249 1104 22-23 0.5718523749813897 1104 24-25 0.5721364916121559 1104 26-27 0.5359501408122895 1104 28-29 0.48284060848691723 1104 30-31 0.4540648207254492 1104 32-33 0.4209739561696537 1104 34-35 0.3108977663293686 1104 36-37 0.42731646225512776 1104 38-39 0.5227507130495113 1104 40-41 0.3574802380511315 1104 42-43 0.3294466768644426 1104 44-45 0.298719011854395 1104 46-47 0.20463123206184974 1104 48-49 0.2864753582374817 1104 50-51 0.33034282075667676 1104 52-53 0.3613149448761348 1104 54-55 0.29750849082069664 1104 56-57 0.3102841867429973 1104 58-59 0.3979291461108758 1104 60-61 0.3645719113241057 1104 62-63 0.4516253435550084 1104 64-65 0.37142931650091526 1104 66-67 0.3162212335720511 1104 68-69 0.2767645290355816 1104 70-71 0.22917733881005375 1104 72-73 0.081356056323024 1104 74-75 0.10774394089138539 1104 76-77 0.0173103766488083 1104 78-79 -0.021569278445873863 1104 80-81 -0.07264389099452728 1104 82-83 0.04258976207335152 1104 84-85 0.13233327254513227 1104 86-87 -0.13710366148619002 1104 88-89 -0.19855359493609726 1104 90-91 -0.21497199355718877 1104 92-93 -0.21071847906304697 1104 94-95 -0.20265433526570575 1104 96-97 -0.3023506575495709 1104 98-99 -0.31109910411537456 1104 100-101 -0.26056210852457795 1105 1 0.14358773483024123 1105 2 0.16076144764154066 1105 3 0.23175749012827396 1105 4 0.23254424264105467 1105 5 0.14425964518303402 1105 6 0.2249604445177269 1105 7 0.17323206845420458 1105 8 0.20313057394721312 1105 9 0.2777771953383237 1105 10-11 0.22553380317667404 1105 12-13 0.3144114376741598 1105 14-15 0.4143935121179041 1105 16-17 0.23304152855460813 1105 18-19 0.23454828097275282 1105 20-21 0.37957168253404916 1105 22-23 0.2417669566717393 1105 24-25 0.2534316813020894 1105 26-27 0.37877989131171574 1105 28-29 0.3897996030544064 1105 30-31 0.3956432951953204 1105 32-33 0.3338451864404348 1105 34-35 0.37567072636134924 1105 36-37 0.31814658841101817 1105 38-39 0.356128101018065 1105 40-41 0.26446110444927484 1105 42-43 0.048502390118315475 1105 44-45 0.21007020172344681 1105 46-47 0.37643529114190954 1105 48-49 0.35031726242601025 1105 50-51 0.27190284410875165 1105 52-53 0.322821766814144 1105 54-55 0.3347458526342635 1105 56-57 0.27134196237575026 1105 58-59 0.2593013370836701 1105 60-61 0.2952344764524071 1105 62-63 0.3117152854982592 1105 64-65 0.3177222588861639 1105 66-67 0.22577665285193405 1105 68-69 0.30303664031613664 1105 70-71 0.15344949404158825 1105 72-73 0.2763192743306533 1105 74-75 0.35658006925809005 1105 76-77 0.4851211577406005 1105 78-79 0.3697009794302666 1105 80-81 0.3334247853098411 1105 82-83 0.29555500182593164 1105 84-85 0.2899020869219129 1105 86-87 0.31430956974819324 1105 88-89 0.3438830430960884 1105 90-91 0.47109395032321544 1105 92-93 0.5017249962485302 1105 94-95 0.24831047161121234 1105 96-97 0.23727778254732357 1105 98-99 0.2840989199150279 1105 100-101 0.13534155673814396 1106 1 0.14121256714340547 1106 2 0.20896525083004747 1106 3 0.23456048801992324 1106 4 0.19609246914584588 1106 5 0.21099655680899332 1106 6 0.1288468588985836 1106 7 0.226353218807418 1106 8 0.3042727145823463 1106 9 0.2871490569917512 1106 10-11 0.16464260425802735 1106 12-13 0.2669035836196656 1106 14-15 0.3913889879985035 1106 16-17 0.375392178510495 1106 18-19 0.3188828349922659 1106 20-21 0.3420699349106684 1106 22-23 0.3284484763870381 1106 24-25 0.36034562942682413 1106 26-27 0.4088246124690258 1106 28-29 0.3099767675622189 1106 30-31 0.2950709947909118 1106 32-33 0.34034984566937254 1106 34-35 0.29627062071389076 1106 36-37 0.33301053780584056 1106 38-39 0.30213448906905427 1106 40-41 0.3654926372755014 1106 42-43 0.3446305967697043 1106 44-45 0.4239273000345847 1106 46-47 0.4381553485779719 1106 48-49 0.4102757954984426 1106 50-51 0.43662387341341713 1106 52-53 0.44311265935115074 1106 54-55 0.34086158440492653 1106 56-57 0.45375704088028357 1106 58-59 0.3705131886403876 1106 60-61 0.4106695261669131 1106 62-63 0.5092377305828748 1106 64-65 0.4196934580528193 1106 66-67 0.4630577048942044 1106 68-69 0.40623592618572246 1106 70-71 0.22094665934145752 1106 72-73 0.2625198000877731 1106 74-75 0.17803306383951067 1106 76-77 0.16205654937924763 1106 78-79 0.2293835730213729 1106 80-81 0.29165213616080976 1106 82-83 0.2531450076803452 1106 84-85 0.34895215585677875 1106 86-87 0.30269404969248015 1106 88-89 0.3068801306904767 1106 90-91 0.307336989081751 1106 92-93 0.35913222919917587 1106 94-95 0.1603851640845022 1106 96-97 0.2671524917017898 1106 98-99 0.27374958925228654 1106 100-101 0.23641359242386528 1107 1 -0.005536924931334397 1107 2 -0.021145592903110355 1107 3 0.05572881525012008 1107 4 0.08481456532339848 1107 5 0.004419940067421635 1107 6 0.08226271705031962 1107 7 0.021401146662789472 1107 8 0.08166521069832555 1107 9 0.08424034671963199 1107 10-11 0.08292163645778317 1107 12-13 0.11996295150292724 1107 14-15 0.10419160048542864 1107 16-17 0.11017482692048475 1107 18-19 0.07529919339258129 1107 20-21 0.10998433371256056 1107 22-23 0.1014858558774634 1107 24-25 0.2006081374873574 1107 26-27 0.05832809535737482 1107 28-29 -0.03733236545297558 1107 30-31 0.12104687791232749 1107 32-33 0.14441769834364493 1107 34-35 0.06384364942551457 1107 36-37 0.07741267080044167 1107 38-39 0.3000813126849877 1107 40-41 0.07318237510560266 1107 42-43 0.11172669852344796 1107 44-45 0.2622340966129926 1107 46-47 0.3824440024973015 1107 48-49 0.3083180822446536 1107 50-51 0.2989455165297912 1107 52-53 0.35146202953159644 1107 54-55 0.37183462812012635 1107 56-57 0.3686519128854471 1107 58-59 0.4114414509743156 1107 60-61 0.2991376111244364 1107 62-63 0.19978618103669987 1107 64-65 0.22324881831147536 1107 66-67 0.17903642936529707 1107 68-69 0.29730553692080974 1107 70-71 0.3189355201623627 1107 72-73 0.354959380594174 1107 74-75 0.46860333943488186 1107 76-77 0.5192722171488988 1107 78-79 0.5716228791878812 1107 80-81 0.5619481573772589 1107 82-83 0.49604182781746786 1107 84-85 0.5688815613383689 1107 86-87 0.4888336466406109 1107 88-89 0.5491284156774618 1107 90-91 0.600408118831151 1107 92-93 0.5922776032992054 1107 94-95 0.5265963661542159 1107 96-97 0.4825942360650508 1107 98-99 0.44909827649820144 1107 100-101 0.5101172897933033 1108 1 0.17159218272512078 1108 2 0.08760962367692571 1108 3 0.15900740057144702 1108 4 0.18116235538207803 1108 5 0.21837823085190422 1108 6 0.22567520404776076 1108 7 0.28139637387180017 1108 8 0.23692146638522615 1108 9 0.33571848448200114 1108 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-1.4699998767191786 2316 52-53 -1.4512814363861466 2316 54-55 -1.3634988106269788 2316 56-57 -1.2250961559390987 2316 58-59 -1.2145329857027107 2316 60-61 -1.2014958869541772 2316 62-63 -1.5061775788694192 2316 64-65 -1.4695416776216348 2316 66-67 -1.390439030744048 2316 68-69 -1.244280340728615 2316 70-71 -1.1995239784646046 2316 72-73 -1.1924214740407209 2316 74-75 -1.151411471683467 2316 76-77 -1.3379337328298497 2316 78-79 -1.1697329981859248 2316 80-81 -0.9865214087483629 2316 82-83 -1.1119201274822572 2316 84-85 -0.9416011128445909 2316 86-87 -0.933910123913261 2316 88-89 -1.02588366096899 2316 90-91 -1.2000523894469737 2316 92-93 -1.4263094073064835 2316 94-95 -1.18083088021082 2316 96-97 -1.1543560951904688 2316 98-99 -1.140971528694248 2316 100-101 -0.9304832350512235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7325.0 3 1780.0 4 1826.0 5 2106.0 6 2455.0 7 2878.0 8 3652.0 9 4394.0 10 5373.0 11 6907.0 12 9049.0 13 14044.0 14 12556.0 15 13104.0 16 16254.0 17 21798.0 18 20231.0 19 13961.0 20 13320.0 21 13721.0 22 14739.0 23 16111.0 24 17923.0 25 20056.0 26 23000.0 27 27145.0 28 31935.0 29 38368.0 30 46029.0 31 56419.0 32 71213.0 33 93342.0 34 132283.0 35 202193.0 36 333481.0 37 521628.0 38 464839.0 39 84882.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92696214165311 18.400145566906442 14.334254639313343 23.338637652127105 2 15.629607153243688 19.85562221889746 40.09923293262482 24.41553769523403 3 19.486128883817976 24.21144863495105 29.215472445825636 27.086950035405334 4 13.385917775449471 15.971221449341874 35.9646147723113 34.67824600289735 5 14.331921146775628 36.89270478631624 33.28521895056563 15.49015511634251 6 32.817286213101454 34.87622305436203 17.104128250922503 15.20236248161401 7 29.28587725110578 32.53819227503228 20.07754631623485 18.09838415762709 8 26.853898732826213 34.23600434142637 19.59129580148101 19.318801124266397 9 27.190334103308935 16.72583630862101 20.090649291938952 35.99318029613111 10-11 25.550256019746342 26.00952656040753 26.369708268781345 22.07050915106478 12-13 26.529336360095645 23.48763770949216 27.104795132643535 22.878230797768666 14-15 23.412216399313802 24.691699337370483 25.702891057925775 26.193193205389942 16-17 22.69673362722797 27.751134663386175 26.49000779933383 23.062123910052023 18-19 22.76755232767006 26.134968938488335 28.913025666451674 22.18445306738993 20-21 23.400053157346797 25.31859214973143 29.072677438908446 22.208677254013327 22-23 23.06273322478315 25.17593178022096 29.072223696594037 22.68911129840186 24-25 22.850587776164012 25.46197123840383 29.09893936123777 22.58850162419439 26-27 22.35422682318921 25.731061991165134 29.025180627182973 22.889530558462685 28-29 22.441199617121114 26.012648452250993 29.01324630649013 22.53290562413776 30-31 22.570043578935866 25.741871498871653 28.707466412281107 22.98061850991137 32-33 22.166273313105723 25.836595054817725 28.755892687069505 23.24123894500705 34-35 22.985455937593578 25.879906720558832 28.50546523689853 22.629172104949056 36-37 23.1260251019196 25.478064506331165 28.550332926104915 22.845577465644322 38-39 22.736870295569254 25.77444458854056 28.499822340029585 22.988862775860596 40-41 22.982267766861053 25.697475777852596 28.644232275409248 22.676024179877103 42-43 22.782188630986315 25.788929022064387 28.29973049823977 23.12915184870953 44-45 22.661595033539637 26.02601971356592 28.37384699261084 22.938538260283607 46-47 23.159499756172053 26.235507423675102 27.715201364761548 22.889791455391297 48-49 22.936540381955037 26.40712338083407 27.50699390240558 23.14934233480531 50-51 23.126949194083522 26.61616743252883 27.39365146458747 22.86323190880018 52-53 23.414487627738705 26.262790744765578 27.41587722909945 22.906844398396263 54-55 22.882061920201995 26.565393981088775 27.53542006132208 23.017124037387152 56-57 22.882585674090965 26.74409778215604 27.342964569824634 23.030351973928358 58-59 22.937240226092367 26.193031039213686 27.442306897706104 23.42742183698784 60-61 22.605953785314593 26.492734675849423 27.708876225445263 23.192435313390728 62-63 22.78850101716775 26.445960841308548 27.87121878142734 22.894319360096365 64-65 22.582876366155375 26.47487404749871 27.657237069143726 23.28501251720219 66-67 22.478908454507405 26.59815184225093 27.568810165120773 23.35412953812089 68-69 22.867419644705148 26.338632507881883 27.721556083346748 23.072391764066225 70-71 22.78562729176613 26.329627830155143 27.34233503450479 23.542409843573942 72-73 22.680931915837053 26.601955797219308 27.261573797202853 23.45553848974079 74-75 22.670425489901554 26.977263943481788 27.16392525446527 23.18838531215138 76-77 22.843122780079135 26.55769895974825 27.346303436289855 23.252874823882763 78-79 22.84049337979329 26.742143011985288 27.220639254616263 23.19672435360516 80-81 22.972120619101293 26.85764655891405 26.94866233589587 23.221570486088776 82-83 22.98221637308497 26.842984409799552 27.021811938989 23.152987278126478 84-85 22.837156781773654 26.91642039851151 26.966088811762827 23.280334007952007 86-87 22.901041569998466 26.92223489571645 26.906458645132226 23.27026488915286 88-89 22.891893853037537 26.85633153249234 26.750820756555715 23.500953857914407 90-91 22.77368360649374 26.902966874764772 26.869275403411592 23.45407411532989 92-93 22.940030246564984 26.617188141101977 26.96652773948379 23.476253872849245 94-95 22.74529758525589 26.42579771911182 27.176171492547418 23.652733203084868 96-97 23.073150088224647 26.04747253897116 27.12279216872299 23.7565852040812 98-99 22.79091323510271 26.37310549128284 26.881746288400937 23.954234985213514 100-101 23.075688347211575 25.896953254950333 27.309357489167883 23.718000908670206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2697.0 1 1587.5 2 545.0 3 634.5 4 616.5 5 629.0 6 980.5 7 1967.5 8 3190.0 9 3697.0 10 3270.5 11 2850.0 12 2970.5 13 3229.5 14 3459.5 15 3647.5 16 3839.0 17 4135.0 18 4341.0 19 4517.5 20 4924.5 21 5505.0 22 6429.0 23 7757.5 24 9230.5 25 11110.0 26 13769.5 27 16511.0 28 18607.0 29 21167.0 30 24147.5 31 28319.5 32 35487.0 33 39148.0 34 39233.0 35 41833.0 36 47626.5 37 52895.0 38 56176.5 39 59009.5 40 61089.0 41 63263.0 42 63926.0 43 66581.5 44 71243.0 45 76449.0 46 79499.5 47 81887.0 48 87329.5 49 87128.5 50 83606.5 51 82710.5 52 83180.0 53 86000.5 54 88178.0 55 84751.5 56 79207.0 57 73877.0 58 70696.5 59 65154.5 60 54108.0 61 45004.5 62 38011.5 63 29249.0 64 21031.0 65 16005.5 66 12126.0 67 9305.0 68 7317.0 69 5823.5 70 4481.5 71 3197.0 72 2470.5 73 2126.0 74 1634.0 75 1213.5 76 921.0 77 610.0 78 434.5 79 312.5 80 196.5 81 152.0 82 129.0 83 89.0 84 61.5 85 48.0 86 36.0 87 27.0 88 22.0 89 20.0 90 17.0 91 14.0 92 11.0 93 7.0 94 4.5 95 7.0 96 6.5 97 3.0 98 3.0 99 3.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34323353102387016 2 0.12135112366271357 3 0.11576838431745554 4 0.12307211849846979 5 0.13809934169848756 6 0.08957568242692172 7 0.09847448499229539 8 0.10409919982135234 9 0.09154853016547156 10-11 0.09047815532859878 12-13 0.09410903467720642 14-15 0.09570410306156586 16-17 0.08443368566155252 18-19 0.10023745531185055 20-21 0.10978687787610768 22-23 0.1174683914113123 24-25 0.13278944299792264 26-27 0.1315091907420552 28-29 0.12594743913869666 30-31 0.11555850689846088 32-33 0.10882144174873223 34-35 0.3346285568450891 36-37 0.49728355656595213 38-39 0.4718254256418996 40-41 0.44920063987427505 42-43 0.48215139465643697 44-45 0.48861561916147256 46-47 0.49610824301958195 48-49 0.5063922365503204 50-51 0.4958144146329895 52-53 0.49862677204751793 54-55 0.48616005335923496 56-57 0.4630315617860233 58-59 0.44363888827091646 60-61 0.4043918109189147 62-63 0.4742600037022377 64-65 0.5039156830061834 66-67 0.5120589268631762 68-69 0.5250503390989458 70-71 0.5166972178229584 72-73 0.5082181700955739 74-75 0.5152490636318952 76-77 0.4937576159268418 78-79 0.47302172693016914 80-81 0.4753933417648088 82-83 0.48788104819499123 84-85 0.4877970972273934 86-87 0.49073538109331866 88-89 0.5024045655894218 90-91 0.516592279113461 92-93 0.5102749688017216 94-95 0.5145144926654138 96-97 0.5144935049235143 98-99 0.5080922436441772 100-101 0.49724158108215316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2382343.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.70129043588925 #Duplication Level Percentage of deduplicated Percentage of total 1 71.6327647069937 32.73709784600036 2 14.330550355586723 13.098492878136096 3 5.4990566254477 7.539419518889591 4 2.6417822244932094 4.829314268397349 5 1.5071364330048147 3.443903992563159 6 0.9082489313566706 2.4904888920010344 7 0.5952095811707495 1.9041292157515894 8 0.4417542088226518 1.6150989918944383 9 0.33611993382506644 1.3825003245328111 >10 1.8726470780226452 15.819332654229932 >50 0.15175236528581135 4.761064789200662 >100 0.07534800246921802 6.087614559862229 >500 0.004504194247512064 1.4015273355521456 >1k 0.0028495922790381027 1.9384532055746104 >5k 1.8384466316374856E-4 0.5312545616387432 >10k+ 9.192233158187428E-5 0.4203069657753934 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10005 0.41996471540831865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7566 0.3175865104227225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5080 0.21323545769857655 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2593 0.10884242949063169 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2523 0.10590414562470643 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2483 0.10422512627274913 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2391 0.10036338176324736 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.19754837989324E-5 0.0 20-21 0.0 0.0 0.0 1.259264513967972E-4 0.0 22-23 0.0 0.0 0.0 2.9382838659252676E-4 0.0 24-25 0.0 0.0 0.0 6.296322569839859E-4 0.0 26-27 0.0 0.0 0.0 0.00104938709497331 0.0 28-29 0.0 0.0 0.0 0.003798781283803382 0.0 30-31 0.0 0.0 0.0 0.011522270302806944 0.0 32-33 0.0 0.0 0.0 0.023926025765391466 0.0 34-35 2.09877418994662E-5 0.0 0.0 0.042416226378821184 0.0 36-37 4.19754837989324E-5 0.0 0.0 0.07274351342354984 0.0 38-39 4.19754837989324E-5 0.0 0.0 0.11834987657108989 0.0 40-41 4.19754837989324E-5 0.0 0.0 0.17230936099461747 0.0 42-43 4.19754837989324E-5 0.0 0.0 0.22450587509858994 0.0 44-45 4.19754837989324E-5 0.0 0.0 0.2921703549824689 0.0 46-47 4.19754837989324E-5 0.0 0.0 0.37091636258926614 0.0 48-49 4.19754837989324E-5 0.0 0.0 0.45925376824411934 0.0 50-51 4.19754837989324E-5 0.0 0.0 0.5589245545246844 0.0 52-53 4.19754837989324E-5 0.0 0.0 0.6550064369404406 0.0 54-55 4.19754837989324E-5 0.0 0.0 0.769326667066833 0.0 56-57 4.19754837989324E-5 0.0 0.0 0.9012766003887769 0.0 58-59 4.19754837989324E-5 0.0 0.0 1.0499117885207965 0.0 60-61 4.19754837989324E-5 0.0 0.0 1.2146655624316063 0.0 62-63 4.19754837989324E-5 0.0 0.0 1.3886959182619798 0.0 64-65 4.19754837989324E-5 0.0 0.0 1.5676793811806276 0.0 66-67 4.19754837989324E-5 0.0 0.0 1.7813765692009924 0.0 68-69 4.19754837989324E-5 0.0 0.0 2.022336833948764 0.0 70-71 4.19754837989324E-5 0.0 0.0 2.291252770906624 0.0 72-73 4.19754837989324E-5 0.0 0.0 2.5715440639739953 0.0 74-75 4.19754837989324E-5 0.0 0.0 2.861552681540819 0.0 76-77 4.19754837989324E-5 0.0 0.0 3.177355233902087 0.0 78-79 4.19754837989324E-5 0.0 0.0 3.5467394913326924 0.0 80-81 4.19754837989324E-5 0.0 0.0 3.943806580328693 0.0 82-83 4.19754837989324E-5 0.0 0.0 4.356677438974993 0.0 84-85 4.19754837989324E-5 0.0 0.0 4.784407618886114 0.0 86-87 4.19754837989324E-5 0.0 0.0 5.234888511016256 0.0 88-89 4.19754837989324E-5 0.0 0.0 5.721426343729681 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6955 0.0 38.563927 1 GTATCAA 11230 0.0 36.0578 1 ATCAACG 13970 0.0 28.624931 3 TATCAAC 14090 0.0 28.448399 2 TCAACGC 14210 0.0 28.108128 4 CAACGCA 14335 0.0 27.829975 5 AACGCAG 14630 0.0 27.290298 6 TAAGGTA 690 0.0 25.406864 4 GTACATG 11170 0.0 24.351818 1 TACATGG 11115 0.0 24.127115 2 ACGCAGA 16510 0.0 24.03931 7 CGCAGAG 16545 0.0 23.902576 8 GAGTACT 8840 0.0 22.904352 12-13 GGACCGA 625 0.0 22.7335 6 ACATGGG 11435 0.0 22.291769 3 GCAGAGT 17775 0.0 21.848885 9 AGTACTT 9190 0.0 21.284758 12-13 GTACTTT 9390 0.0 21.26081 14-15 CATGGGG 5780 0.0 20.329292 4 TAAGGTG 1280 0.0 19.618433 5 >>END_MODULE