FastQCFastQC Report
Fri 27 May 2016
SRR939093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382343
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102240.4291573463602848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81270.3411347568339236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.2246527892918862No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26540.11140293400236657No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25460.10686958175208187No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23890.1002794307956495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69700.041.2644541
GTATCAA113700.036.5616461
TATCAAC144150.028.7032182
ATCAACG144450.028.6436083
TCAACGC145550.028.4923324
CAACGCA146800.028.21745
AACGCAG151500.027.3106886
ACGCAGA171300.024.1816357
CGCAGAG172700.023.9581328
GTGTAAG9100.023.0241321
TACATGG109700.022.8378942
GTACATG112850.022.7857931
GAGTACT98300.021.96505412-13
GCAGAGT187850.021.7995059
ACATGGG114200.021.4393883
GTACTTT103150.020.74825914-15
TAAGGTA7800.020.682984
AGTACTT102400.019.90387212-13
TAAGGTG12300.019.6740535
AGAGTAC167450.019.2140510-11
Produced by FastQC (version 0.11.5)