##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939093_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2382343 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51292949839716 34.0 31.0 34.0 31.0 34.0 2 32.71909460560465 34.0 31.0 34.0 31.0 34.0 3 32.805185063611745 34.0 31.0 34.0 31.0 34.0 4 36.268375712481365 37.0 37.0 37.0 35.0 37.0 5 36.23055454231402 37.0 37.0 37.0 35.0 37.0 6 36.26669837214876 37.0 37.0 37.0 35.0 37.0 7 36.21960187932636 37.0 37.0 37.0 35.0 37.0 8 36.20615293431718 37.0 37.0 37.0 35.0 37.0 9 37.95225876374645 39.0 38.0 39.0 35.0 39.0 10-11 37.896964458938115 39.0 38.0 39.0 35.0 39.0 12-13 37.83147829678598 39.0 38.0 39.0 35.0 39.0 14-15 39.19863323627202 40.5 39.0 41.0 36.0 41.0 16-17 39.14136713311224 40.0 39.0 41.0 36.0 41.0 18-19 39.173961096282106 40.5 38.5 41.0 36.0 41.0 20-21 39.230250220056476 41.0 39.0 41.0 36.0 41.0 22-23 39.18989813809347 41.0 39.0 41.0 36.0 41.0 24-25 39.12815996688974 41.0 39.0 41.0 35.0 41.0 26-27 38.987726368537196 40.0 38.5 41.0 35.0 41.0 28-29 38.83086503496767 40.0 38.0 41.0 35.0 41.0 30-31 38.61806276426191 40.0 38.0 41.0 34.5 41.0 32-33 38.44916747924207 40.0 38.0 41.0 34.0 41.0 34-35 38.39187514140491 40.0 38.0 41.0 34.0 41.0 36-37 38.268775948719394 40.0 38.0 41.0 34.0 41.0 38-39 38.11469758972575 40.0 38.0 41.0 33.5 41.0 40-41 37.91718719764535 40.0 37.5 41.0 33.0 41.0 42-43 37.7251921742587 40.0 37.0 41.0 33.0 41.0 44-45 37.479463074796534 40.0 37.0 41.0 33.0 41.0 46-47 37.153332034891704 40.0 37.0 41.0 32.0 41.0 48-49 36.79233720753057 40.0 36.0 41.0 31.0 41.0 50-51 36.50871767835278 39.5 36.0 41.0 30.5 41.0 52-53 36.2386379711066 39.0 35.0 41.0 30.0 41.0 54-55 35.967000763534045 39.0 35.0 41.0 29.5 41.0 56-57 35.66434199441474 38.5 35.0 40.0 29.0 41.0 58-59 35.33035188467824 38.0 35.0 40.0 28.0 41.0 60-61 34.951876576966455 37.5 34.5 40.0 28.0 41.0 62-63 34.536604510769436 37.0 34.0 40.0 26.5 41.0 64-65 34.11367884473395 36.5 34.0 39.0 26.0 41.0 66-67 33.74870705016028 36.0 33.5 39.0 26.0 40.5 68-69 33.281487384478226 35.0 33.0 38.5 25.5 40.0 70-71 32.84322114825615 35.0 33.0 38.0 24.5 40.0 72-73 32.38852402865582 35.0 32.0 37.0 23.0 39.5 74-75 31.9176065327285 35.0 32.0 37.0 21.5 39.0 76-77 29.9846768496392 33.5 29.5 35.0 19.0 37.0 78-79 30.957953577633447 34.0 31.0 35.5 19.5 37.5 80-81 31.014881148516395 35.0 31.0 35.0 19.5 37.0 82-83 30.751542284213482 34.5 31.0 35.0 18.0 37.0 84-85 30.40597743481942 34.0 31.0 35.0 15.5 36.0 86-87 29.887318282883697 34.0 30.5 35.0 7.5 36.0 88-89 29.358687015261864 34.0 30.0 35.0 2.0 35.5 90-91 28.92400884339493 34.0 29.0 35.0 2.0 35.0 92-93 28.657206372046346 34.0 29.0 35.0 2.0 35.0 94-95 28.352335075175993 34.0 29.0 35.0 2.0 35.0 96-97 27.818015709744564 34.0 29.0 35.0 2.0 35.0 98-99 27.060779241276343 33.5 27.0 35.0 2.0 35.0 100-101 23.436497389334786 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2009928562076766 1101 2 0.15384892119989502 1101 3 0.12483900956688387 1101 4 0.09773974443034916 1101 5 0.16336176650668222 1101 6 0.16829263761055557 1101 7 0.131627258982995 1101 8 0.19144186558811072 1101 9 0.12349834821929306 1101 10-11 0.18286498066358092 1101 12-13 0.1948721857662079 1101 14-15 0.20445074544075936 1101 16-17 0.24358668268026662 1101 18-19 0.2453961050380613 1101 20-21 0.2080755673360173 1101 22-23 0.2267512659831894 1101 24-25 0.16477526279717836 1101 26-27 0.2578586158893401 1101 28-29 0.1738925265454938 1101 30-31 0.30379781028486263 1101 32-33 0.3047040849584306 1101 34-35 0.5111754127597266 1101 36-37 0.4055013029870196 1101 38-39 0.4119919880401781 1101 40-41 0.38539276014262924 1101 42-43 0.298487290396821 1101 44-45 0.3425459547649794 1101 46-47 0.41873704273671564 1101 48-49 0.26158666255263086 1101 50-51 0.26293465677240846 1101 52-53 0.3288684815905967 1101 54-55 0.28013626606773556 1101 56-57 0.3125758168198942 1101 58-59 0.3422072844723587 1101 60-61 0.30116502199538786 1101 62-63 0.18182256720462675 1101 64-65 0.11971110434739529 1101 66-67 0.13424096943477082 1101 68-69 0.10171881244518488 1101 70-71 0.16562168131245159 1101 72-73 0.07179704081058702 1101 74-75 0.22073624134571546 1101 76-77 0.1999156447192192 1101 78-79 0.19071928394107118 1101 80-81 0.2096506474696902 1101 82-83 0.23879183574140583 1101 84-85 0.2980364057953828 1101 86-87 0.26390341619793034 1101 88-89 0.28045787107703646 1101 90-91 0.2720877313789565 1101 92-93 0.21450699558287312 1101 94-95 0.14772425112927579 1101 96-97 0.17357033670571553 1101 98-99 0.21899550752084096 1101 100-101 -0.07149327500522062 1102 1 0.15653856081564754 1102 2 0.11766942177958839 1102 3 0.18886495923612046 1102 4 0.10009118456311228 1102 5 0.1297667200177841 1102 6 0.07939683411422749 1102 7 0.09574897122923431 1102 8 0.060647803436211234 1102 9 0.19757084361587118 1102 10-11 0.22207009444203152 1102 12-13 0.16887136737978636 1102 14-15 0.22119214670016873 1102 16-17 0.2348060784382895 1102 18-19 0.1715516496060232 1102 20-21 0.20699930395198152 1102 22-23 0.20981095135908134 1102 24-25 0.18709618393655347 1102 26-27 0.08768823238437307 1102 28-29 0.08986086522106973 1102 30-31 0.1869767015986028 1102 32-33 0.16818415952253218 1102 34-35 0.21981635140347322 1102 36-37 0.2856673672303529 1102 38-39 0.18750688949281624 1102 40-41 0.2483918906070599 1102 42-43 0.11650500505275829 1102 44-45 0.2067271989396673 1102 46-47 0.21446012123360703 1102 48-49 0.35901371818017935 1102 50-51 0.432121129526962 1102 52-53 0.47281497957872887 1102 54-55 0.4782333755723869 1102 56-57 0.4662505991518415 1102 58-59 0.48418880257914765 1102 60-61 0.40129374735854384 1102 62-63 0.4527141537376238 1102 64-65 0.47331157832952186 1102 66-67 0.41781257256283055 1102 68-69 0.4732221996556234 1102 70-71 0.4123801038726427 1102 72-73 0.32986155693961905 1102 74-75 0.2868038946226683 1102 76-77 0.49393517232839557 1102 78-79 0.45478743165981683 1102 80-81 0.3715235020469727 1102 82-83 0.34340179504941304 1102 84-85 0.26325143114262417 1102 86-87 0.4609967236156898 1102 88-89 0.4671124892770422 1102 90-91 0.49482949636566076 1102 92-93 0.6199748398166598 1102 94-95 0.44192848400567897 1102 96-97 0.5190771679589865 1102 98-99 0.42165625793571593 1102 100-101 0.5342341511695032 1103 1 0.1265196708323586 1103 2 0.12495801626624115 1103 3 0.053058442517418314 1103 4 0.08899622589134282 1103 5 0.13085166169693707 1103 6 0.07355405346404353 1103 7 0.1340446687588468 1103 8 0.10266336744312099 1103 9 0.2037661256774541 1103 10-11 0.20635797183477678 1103 12-13 0.20738702951043564 1103 14-15 0.2906223686272398 1103 16-17 0.25814092780672127 1103 18-19 0.2398262435550862 1103 20-21 0.23161604004933878 1103 22-23 0.24004748073621585 1103 24-25 0.15789274411802978 1103 26-27 0.23873415708673207 1103 28-29 0.3224625023529555 1103 30-31 0.34168785040906613 1103 32-33 0.24241291154662292 1103 34-35 0.3517395395787801 1103 36-37 0.48491902254536257 1103 38-39 0.4331429406213303 1103 40-41 0.4900164942755012 1103 42-43 0.5380411206360165 1103 44-45 0.49194432213751327 1103 46-47 0.5708176562535598 1103 48-49 0.6845764415744569 1103 50-51 0.5172451049244131 1103 52-53 0.6529631975864376 1103 54-55 0.4827038189335937 1103 56-57 0.41705549287592447 1103 58-59 0.5675012090476912 1103 60-61 0.6944463376858678 1103 62-63 0.6066486833435576 1103 64-65 0.5909361373539852 1103 66-67 0.6010681974715624 1103 68-69 0.4392374362733591 1103 70-71 0.48682440260410687 1103 72-73 0.5665639294880336 1103 74-75 0.43733873878263196 1103 76-77 0.33260857651544384 1103 78-79 0.48404388620248184 1103 80-81 0.4171173274127504 1103 82-83 0.5279816254581 1103 84-85 0.614572567369077 1103 86-87 0.8334242306308433 1103 88-89 0.826788792532426 1103 90-91 0.8748806602248678 1103 92-93 0.763737518008913 1103 94-95 0.5504414860992917 1103 96-97 0.4718982488455019 1103 98-99 0.4611599006053133 1103 100-101 0.10809505541468312 1104 1 0.13838628497152428 1104 2 0.15349786301020885 1104 3 0.17038970523525876 1104 4 0.09781407562867628 1104 5 0.13731808483765207 1104 6 0.10447258717280761 1104 7 0.13826870677562653 1104 8 0.15720652764087362 1104 9 0.08439297344676078 1104 10-11 0.1579017880401068 1104 12-13 0.13805270132022684 1104 14-15 0.13926049339530522 1104 16-17 0.17086927735850566 1104 18-19 0.15784156564073726 1104 20-21 0.15049709866617178 1104 22-23 0.23642954622793155 1104 24-25 0.10978808716777877 1104 26-27 0.1521095006992752 1104 28-29 0.10472734650279136 1104 30-31 0.10654475106350247 1104 32-33 0.20060100281082072 1104 34-35 0.2421022959361565 1104 36-37 0.2408890431753008 1104 38-39 0.2081167354932134 1104 40-41 0.3394484673457683 1104 42-43 0.3923098304819348 1104 44-45 0.18600758553774455 1104 46-47 0.3550190857520832 1104 48-49 0.19402721550510194 1104 50-51 0.2160679406710173 1104 52-53 0.1127447013386913 1104 54-55 0.22261242635087797 1104 56-57 0.30433848715476586 1104 58-59 0.3213246955256679 1104 60-61 0.3980705156978317 1104 62-63 0.3101235888556104 1104 64-65 0.22872586670249717 1104 66-67 0.27323896697020444 1104 68-69 0.18919588619606031 1104 70-71 0.3332183835148115 1104 72-73 0.2843663568919155 1104 74-75 0.2645737372261543 1104 76-77 0.27682067273223154 1104 78-79 0.4611715579384068 1104 80-81 0.38337250908111997 1104 82-83 0.3712061488853102 1104 84-85 0.37795542956703443 1104 86-87 0.30800151905081563 1104 88-89 0.3917863039246221 1104 90-91 0.46270785059545005 1104 92-93 0.4374038704485379 1104 94-95 0.45260754219233945 1104 96-97 0.5668056603240537 1104 98-99 0.4876495038526407 1104 100-101 0.32178996089155376 1105 1 -0.010130079304126127 1105 2 0.11147409465342406 1105 3 0.09307288751820408 1105 4 0.11459277582413563 1105 5 0.09041803848180763 1105 6 0.05639229122517264 1105 7 0.05381835520927325 1105 8 0.11078206402937951 1105 9 -0.0390093463355754 1105 10-11 0.07397822625290473 1105 12-13 0.09107916146705719 1105 14-15 0.10011323357288404 1105 16-17 0.15607220648382025 1105 18-19 0.1697804754408878 1105 20-21 0.20131707463410464 1105 22-23 0.13394384895396882 1105 24-25 0.0512884035318919 1105 26-27 0.16957338004471723 1105 28-29 0.1013325481424161 1105 30-31 0.22475484336884222 1105 32-33 0.1687752087286114 1105 34-35 0.3466498537959595 1105 36-37 0.2778590710966071 1105 38-39 0.4012104820984135 1105 40-41 0.276241260878173 1105 42-43 0.1952247339266151 1105 44-45 0.3234181390795712 1105 46-47 0.23377233335613568 1105 48-49 0.3181915249896434 1105 50-51 0.31975731423690945 1105 52-53 0.3302293744719549 1105 54-55 0.3285236047429194 1105 56-57 0.3285752742885748 1105 58-59 0.42649917675901605 1105 60-61 0.40427662173246404 1105 62-63 0.3372786586837577 1105 64-65 0.27470128400872795 1105 66-67 0.42541694520866713 1105 68-69 0.22211838155011066 1105 70-71 0.40408995627150546 1105 72-73 0.3351438330941221 1105 74-75 0.2969592843204545 1105 76-77 0.3579448475111704 1105 78-79 0.34006961571984107 1105 80-81 0.26976337921925264 1105 82-83 0.3323869665227548 1105 84-85 0.27978107438177346 1105 86-87 0.38489116229326825 1105 88-89 0.5108119771001789 1105 90-91 0.48147678372279756 1105 92-93 0.41210707696257387 1105 94-95 0.1420563637671286 1105 96-97 0.13731463751846817 1105 98-99 0.11701298589783704 1105 100-101 -0.20638240820709441 1106 1 0.04595928060468424 1106 2 0.01212808082484429 1106 3 0.06062739686656471 1106 4 0.018055360282595245 1106 5 0.004537975349350631 1106 6 0.0792419044078656 1106 7 0.0783218851584877 1106 8 0.007517373542228256 1106 9 0.028341408627731823 1106 10-11 0.037951282750697146 1106 12-13 0.09390086067902814 1106 14-15 0.1509071196212446 1106 16-17 0.05993521921252665 1106 18-19 0.07009393757131477 1106 20-21 0.1969214643040189 1106 22-23 0.113971238887693 1106 24-25 0.07321435150382172 1106 26-27 0.14593461114763073 1106 28-29 0.16690167732293304 1106 30-31 0.22225676700465158 1106 32-33 0.29288307074366315 1106 34-35 0.0952789070071205 1106 36-37 0.30675611122764224 1106 38-39 0.29051716715038367 1106 40-41 0.13901827293522473 1106 42-43 0.22310710242182807 1106 44-45 0.29984387473650287 1106 46-47 0.31981580802544585 1106 48-49 0.3080621098529335 1106 50-51 0.3270269274716071 1106 52-53 0.3923845626282443 1106 54-55 0.4088602765119518 1106 56-57 0.29221790745847187 1106 58-59 0.3612799722831781 1106 60-61 0.2711446226133276 1106 62-63 0.3380070101053647 1106 64-65 0.29898449840366226 1106 66-67 0.2907510377956015 1106 68-69 0.19220596973383408 1106 70-71 0.17024534684207993 1106 72-73 0.1721091228435796 1106 74-75 0.17656868474412235 1106 76-77 0.2550142806131461 1106 78-79 0.4472275810721982 1106 80-81 0.29874652542937596 1106 82-83 0.10669633276401314 1106 84-85 0.2935518465982945 1106 86-87 0.41711413723529844 1106 88-89 0.5837157241047173 1106 90-91 0.6048734955306507 1106 92-93 0.5714171242519122 1106 94-95 0.45496175488773716 1106 96-97 0.458448009534532 1106 98-99 0.5947879168079595 1106 100-101 0.31069629754953 1107 1 -0.10620218579606444 1107 2 -0.07463725439805557 1107 3 -0.0513532648530699 1107 4 -0.002098982143188266 1107 5 -0.01921217089658711 1107 6 -0.0024768284045961764 1107 7 -0.06202757364408029 1107 8 0.03385855084050604 1107 9 0.06864426307638638 1107 10-11 0.04537727258791335 1107 12-13 0.0557039477607546 1107 14-15 0.10525069204903303 1107 16-17 0.02223573683568958 1107 18-19 -0.08922745794727405 1107 20-21 -0.016218048297623966 1107 22-23 0.01873938125525143 1107 24-25 -0.050539545634656236 1107 26-27 0.05570757137146387 1107 28-29 0.02838296503349369 1107 30-31 0.04485364012069937 1107 32-33 0.09450711003902512 1107 34-35 -0.012660993977092971 1107 36-37 0.06578546186457856 1107 38-39 0.1308769457660972 1107 40-41 0.1200597461458841 1107 42-43 0.18629180064601059 1107 44-45 0.14528232297865884 1107 46-47 0.23883860528951573 1107 48-49 0.12756012382335769 1107 50-51 0.11351660124500995 1107 52-53 0.08334046107194837 1107 54-55 0.047438944777958625 1107 56-57 0.09228425744357338 1107 58-59 0.05165069262168487 1107 60-61 0.23750679324901114 1107 62-63 0.13142145341266342 1107 64-65 -0.02238206252540209 1107 66-67 0.10013932747023091 1107 68-69 0.029548320497866598 1107 70-71 0.09779792638723706 1107 72-73 0.14306992659960116 1107 74-75 0.16011635664481005 1107 76-77 0.07418404289984792 1107 78-79 0.2827180015258719 1107 80-81 0.3591922558447571 1107 82-83 0.279202698592254 1107 84-85 0.18302897995243583 1107 86-87 0.2819657865202423 1107 88-89 0.3615406417697109 1107 90-91 0.48065549832069365 1107 92-93 0.32612527177354167 1107 94-95 0.19478838202686788 1107 96-97 0.1990327833984722 1107 98-99 0.5167264013929547 1107 100-101 -0.11068275987643617 1108 1 0.016488921659806977 1108 2 0.0021882100523384906 1108 3 -0.01843637949448862 1108 4 -0.0027340122329988503 1108 5 0.030557351612024775 1108 6 0.057277123971445576 1108 7 0.09196447804846741 1108 8 0.06312483670872382 1108 9 0.007870968452436955 1108 10-11 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-0.5659990008151574 2316 96-97 -0.770660854203495 2316 98-99 -0.8088544744213984 2316 100-101 -0.43546135469153313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 10.0 5 34.0 6 82.0 7 268.0 8 645.0 9 1141.0 10 1850.0 11 3052.0 12 4546.0 13 6689.0 14 8004.0 15 11084.0 16 14836.0 17 21453.0 18 21224.0 19 14424.0 20 12503.0 21 12756.0 22 13453.0 23 14444.0 24 16044.0 25 18997.0 26 22290.0 27 26613.0 28 32431.0 29 39797.0 30 48584.0 31 59831.0 32 75140.0 33 98391.0 34 138339.0 35 208870.0 36 347233.0 37 570617.0 38 470219.0 39 46441.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24277420368496 18.86969367265522 14.771175920765922 23.116356202893897 2 15.94871099585576 20.261482078777068 39.45666094260986 24.333145982757312 3 19.490266514939282 24.261325929977335 29.494409495190236 26.753998059893142 4 13.580748028306589 16.45707608014463 35.69855390260764 34.26362198894114 5 14.290217655476143 37.0565867299545 33.262590651304194 15.390604963265156 6 32.89089774226465 35.215206206662934 16.75249953512152 15.141396515950895 7 29.23575656402122 32.67535363295713 19.98221918506277 18.106670617958876 8 26.954263093097847 34.458514160219586 19.31157688040723 19.275645866275344 9 26.993971900771633 16.74960322673939 19.993132810850494 36.26329206163848 10-11 25.566385696769945 26.195598198915942 26.221266207258985 22.016749897055128 12-13 26.566178757634816 23.575005782122894 27.033218978123635 22.825596482118655 14-15 23.311044728254128 24.851672233046223 25.621849563144323 26.21543347555533 16-17 22.58222197782732 27.890913029042093 26.47960941963253 23.04725557349806 18-19 22.649838404779498 26.180734374144095 29.02079739617515 22.14862982490126 20-21 23.27909960908232 25.292012946918224 29.288834563284965 22.14005288071449 22-23 22.97981440959593 25.18157544904323 29.283986394906194 22.554623746454645 24-25 22.80536281707404 25.438415332888393 29.289204266411456 22.467017583626113 26-27 22.337593268056448 25.7100293475229 29.216788638439063 22.735588745981588 28-29 22.45180479888916 26.021735745020763 29.16423033962784 22.362229116462238 30-31 22.540025882865553 25.74979924618148 28.918456380487356 22.79171849046561 32-33 22.162614703256416 25.75569512870313 28.94979858064099 23.131891587399465 34-35 22.949048944414358 25.864220075647854 28.716043474427916 22.47068750550987 36-37 23.09592388566054 25.48398609384972 28.71848862987471 22.70160139061503 38-39 22.72763150547417 25.76845615182669 28.674279049517153 22.82963329318198 40-41 22.978914731350745 25.7248211968127 28.757228375850147 22.53903569598641 42-43 22.782104830325597 25.811713288217803 28.401039164285898 23.005142717170706 44-45 22.657247607525957 25.985743801479433 28.54134012127969 22.815668469714918 46-47 23.117247545449583 26.29588508632378 27.79686522744731 22.79000214077933 48-49 22.981006797558354 26.44486942593079 27.545285573846733 23.02883820266412 50-51 23.089054001924996 26.61510253989668 27.521941653189025 22.773901804989293 52-53 23.4580817775457 26.240659922320102 27.536507158295137 22.764751141839056 54-55 22.886901676206996 26.56208614796442 27.59843985521816 22.95257232061042 56-57 22.883627588470677 26.730953519287525 27.354037600798875 23.03138129144292 58-59 22.937697885063212 26.207228753821276 27.48227757902547 23.372795782090044 60-61 22.62276232387936 26.538923799684568 27.709905910869516 23.12840796556656 62-63 22.790673659519058 26.5678717779302 27.80057609596338 22.84087846658736 64-65 22.52686329371255 26.500219221519096 27.656227076974353 23.316690407794002 66-67 22.443484424228192 26.617389546777687 27.597202046895745 23.341923982098372 68-69 22.858565277144624 26.35354776854564 27.726992626323426 23.060894327986308 70-71 22.758550199761412 26.390879227247808 27.329304524707197 23.52126604828358 72-73 22.696144090082747 26.62036910721924 27.21868765328922 23.464799149408798 74-75 22.605812848947444 26.991600285936997 27.080966090944926 23.321620774170636 76-77 22.759631178100207 26.49319885500896 27.440725427420766 23.30644453947007 78-79 22.703939054763698 26.710679499172134 27.30167518386894 23.283706262195228 80-81 22.807066148754995 26.85139885054504 26.975440752781132 23.366094247918834 82-83 22.82170391002241 26.734643635118864 27.134399923771994 23.309252531086734 84-85 22.655826637893874 26.857635529392702 26.996973147863258 23.489564684850166 86-87 22.750990245046253 26.790215126691695 26.95956320801575 23.4992314202463 88-89 22.661586156769598 26.802534691423507 26.81589558106488 23.719983570742013 90-91 22.525310183683864 26.736850578098565 26.962249071129374 23.775590167088197 92-93 22.57132998900662 26.57545533955438 27.027048582005193 23.826166089433805 94-95 22.375829173213095 26.29510108326131 27.249434695171935 24.07963504835366 96-97 22.58354485479211 25.928067452923447 27.20250610428473 24.285881587999715 98-99 22.24898664467679 26.250021775097558 26.991823267221687 24.50916831300396 100-101 22.409458007320595 25.651570737768225 27.63209812283824 24.306873132072937 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 759.0 1 536.0 2 435.0 3 582.0 4 498.0 5 459.0 6 856.5 7 1811.0 8 2986.5 9 3476.0 10 2994.5 11 2527.5 12 2562.0 13 2698.5 14 2882.0 15 3105.0 16 3275.0 17 3454.5 18 3668.5 19 4042.0 20 4566.0 21 5210.0 22 6145.5 23 7462.0 24 8957.5 25 10799.0 26 13358.0 27 16277.0 28 18803.5 29 21359.0 30 24355.5 31 28872.5 32 36267.0 33 39971.0 34 39850.0 35 42409.0 36 48169.5 37 53072.5 38 56272.0 39 59024.5 40 61184.5 41 63641.5 42 64536.0 43 67508.5 44 72171.5 45 76976.0 46 79602.5 47 82475.5 48 88214.0 49 87822.0 50 84724.0 51 83702.5 52 84028.0 53 87017.0 54 89347.0 55 85283.5 56 79371.5 57 74275.5 58 70711.0 59 65066.5 60 53658.5 61 44552.0 62 37880.5 63 28890.5 64 20476.0 65 15363.0 66 11523.5 67 8966.5 68 7059.0 69 5518.5 70 4175.5 71 2946.0 72 2251.0 73 1997.5 74 1565.5 75 1108.5 76 824.0 77 551.0 78 370.5 79 230.5 80 128.0 81 74.5 82 51.0 83 28.0 84 18.5 85 16.0 86 10.5 87 7.0 88 4.5 89 3.5 90 1.5 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34898417230432394 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014481541910631676 16-17 0.0017629703195551605 18-19 6.29632256983986E-5 20-21 0.0 22-23 0.0 24-25 5.456812893861212E-4 26-27 0.0012382767720685057 28-29 0.0 30-31 0.0030222348335231324 32-33 0.0 34-35 0.010619797401129895 36-37 0.02782974575869218 38-39 0.021911202543042713 40-41 0.013621044492753562 42-43 0.00992720191844751 44-45 0.0034000141877135242 46-47 0.001385190965364769 48-49 7.135832245818507E-4 50-51 8.39509675978648E-5 52-53 8.39509675978648E-5 54-55 0.0 56-57 0.0 58-59 0.005058045797771354 60-61 0.010074116111743775 62-63 0.004239523863692172 64-65 0.0020777864480471535 66-67 0.004260511605591638 68-69 0.003567916122909254 70-71 4.19754837989324E-5 72-73 0.0 74-75 0.0 76-77 0.21854535639914152 78-79 3.1481612849199297E-4 80-81 0.003504952897210855 82-83 6.925954826823845E-4 84-85 0.0 86-87 0.11035354690739325 88-89 0.4091140528462946 90-91 0.39601770190102764 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014481541910631676 100-101 9.65436127375445E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2382343.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.18646522980149 #Duplication Level Percentage of deduplicated Percentage of total 1 70.27213638818803 31.050773111405388 2 14.933274488700587 13.196972279241004 3 5.770892946673314 7.6498608159926125 4 2.7997114651269976 4.948374132292427 5 1.52722947411685 3.37414360279961 6 0.9756791730908614 2.586708831433249 7 0.6416962197875562 1.984800139261653 8 0.482722386289779 1.70638367499521 9 0.3503785649071172 1.3933791247982474 >10 1.994599287757537 16.373658801008972 >50 0.1619750081378786 4.94451246041242 >100 0.08181488615293823 6.3898182088641455 >500 0.0047528379943329565 1.4799902998850916 >1k 0.0028517027965997113 1.9250458458489157 >5k 1.901135197733141E-4 0.56614795243929 >10k+ 9.505675988665705E-5 0.4294307193218562 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10224 0.4291573463602848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8127 0.3411347568339236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5352 0.2246527892918862 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2654 0.11140293400236657 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2546 0.10686958175208187 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2389 0.1002794307956495 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.19754837989324E-5 0.0 12-13 0.0 0.0 0.0 4.19754837989324E-5 0.0 14-15 0.0 0.0 0.0 4.19754837989324E-5 0.0 16-17 0.0 0.0 0.0 4.19754837989324E-5 0.0 18-19 0.0 0.0 0.0 8.39509675978648E-5 0.0 20-21 0.0 0.0 0.0 1.259264513967972E-4 0.0 22-23 0.0 0.0 0.0 3.1481612849199297E-4 0.0 24-25 0.0 0.0 0.0 6.716077407829184E-4 0.0 26-27 0.0 0.0 0.0 0.0010913625787722424 0.0 28-29 0.0 0.0 0.0 0.003735818058104983 0.0 30-31 0.0 0.0 0.0 0.01148029481900801 0.0 32-33 0.0 0.0 0.0 0.02400997673298933 0.0 34-35 0.0 0.0 0.0 0.04268906702351424 0.0 36-37 0.0 0.0 0.0 0.07331018245483542 0.0 38-39 0.0 0.0 0.0 0.1193363004403648 0.0 40-41 0.0 0.0 0.0 0.17407233131417266 0.0 42-43 0.0 0.0 0.0 0.22685650219133013 0.0 44-45 4.19754837989324E-5 0.0 0.0 0.2952975285254894 0.0 46-47 4.19754837989324E-5 0.0 0.0 0.37490403355016466 0.0 48-49 4.19754837989324E-5 0.0 0.0 0.46395502242959974 0.0 50-51 4.19754837989324E-5 0.0 0.0 0.5642344532252492 0.0 52-53 4.19754837989324E-5 0.0 0.0 0.6609039924141906 0.0 54-55 4.19754837989324E-5 0.0 0.0 0.7753921244757787 0.0 56-57 4.19754837989324E-5 0.0 0.0 0.9081605797318018 0.0 58-59 4.19754837989324E-5 0.0 0.0 1.0582439220548845 0.0 60-61 4.19754837989324E-5 0.0 0.0 1.2238581933835724 0.0 62-63 4.19754837989324E-5 0.0 0.0 1.399420654372607 0.0 64-65 4.19754837989324E-5 0.0 0.0 1.5796423940633235 0.0 66-67 4.19754837989324E-5 0.0 0.0 1.79445193240436 0.0 68-69 4.19754837989324E-5 0.0 0.0 2.0354121971521315 0.0 70-71 4.19754837989324E-5 0.0 0.0 2.3046429502384838 0.0 72-73 4.19754837989324E-5 0.0 0.0 2.5854799245952407 0.0 74-75 4.19754837989324E-5 0.0 0.0 2.8764959537732393 0.0 76-77 4.19754837989324E-5 0.0 0.0 3.1940195009702634 0.0 78-79 4.19754837989324E-5 0.0 0.0 3.564558084205339 0.0 80-81 4.19754837989324E-5 0.0 0.0 3.962485670619218 0.0 82-83 4.19754837989324E-5 0.0 0.0 4.3782528376476435 0.0 84-85 4.19754837989324E-5 0.0 0.0 4.8090262401341874 0.0 86-87 4.19754837989324E-5 0.0 0.0 5.2617108451637735 0.0 88-89 4.19754837989324E-5 0.0 0.0 5.750872145614633 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6970 0.0 41.264454 1 GTATCAA 11370 0.0 36.561646 1 TATCAAC 14415 0.0 28.703218 2 ATCAACG 14445 0.0 28.643608 3 TCAACGC 14555 0.0 28.492332 4 CAACGCA 14680 0.0 28.2174 5 AACGCAG 15150 0.0 27.310688 6 ACGCAGA 17130 0.0 24.181635 7 CGCAGAG 17270 0.0 23.958132 8 GTGTAAG 910 0.0 23.024132 1 TACATGG 10970 0.0 22.837894 2 GTACATG 11285 0.0 22.785793 1 GAGTACT 9830 0.0 21.965054 12-13 GCAGAGT 18785 0.0 21.799505 9 ACATGGG 11420 0.0 21.439388 3 GTACTTT 10315 0.0 20.748259 14-15 TAAGGTA 780 0.0 20.68298 4 AGTACTT 10240 0.0 19.903872 12-13 TAAGGTG 1230 0.0 19.674053 5 AGAGTAC 16745 0.0 19.21405 10-11 >>END_MODULE