FastQCFastQC Report
Fri 27 May 2016
SRR939092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345275
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95660.40788393685175517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73520.3134813614608095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48150.20530641395998336No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26270.11201245056549872No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25450.10851605888435258No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25120.10710897442730598No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23680.10096896952382983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68450.039.338121
GTATCAA112300.036.706581
ATCAACG139600.029.11273
TATCAAC141000.028.9580142
TCAACGC141250.028.8396934
CAACGCA142350.028.6494985
AACGCAG146750.027.8384156
ACGCAGA165250.024.5499827
CGCAGAG167600.024.1482358
TACATGG112650.022.7481822
TAAGGTG10850.022.7009985
GTACATG114450.022.6973671
GCAGAGT178050.022.3055679
GAGTACT89100.022.04936612-13
ACATGGG113600.021.5572243
GTACTTT94100.020.80364414-15
AGTACTT92300.020.54123512-13
GTAAGGT9850.020.1973154
GTATAGG5800.019.6511171
AGAGTAC158300.019.42230810-11
Produced by FastQC (version 0.11.5)