##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939092_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2345275 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19339139333341 33.0 31.0 34.0 31.0 34.0 2 32.28769419364467 34.0 31.0 34.0 30.0 34.0 3 32.34876890769739 34.0 31.0 34.0 30.0 34.0 4 35.79247124537634 37.0 35.0 37.0 35.0 37.0 5 35.83026766583876 37.0 35.0 37.0 35.0 37.0 6 35.916519384720345 37.0 37.0 37.0 35.0 37.0 7 35.85133001460383 37.0 37.0 37.0 35.0 37.0 8 35.8463331592244 37.0 37.0 37.0 35.0 37.0 9 37.5392084083956 39.0 38.0 39.0 35.0 39.0 10-11 37.44805769046273 39.0 38.0 39.0 35.0 39.0 12-13 37.35673087377813 39.0 37.5 39.0 35.0 39.0 14-15 38.663696581424354 40.0 38.0 41.0 34.5 41.0 16-17 38.57839016746437 40.0 38.0 41.0 34.0 41.0 18-19 38.488607732568674 40.0 38.0 41.0 34.0 41.0 20-21 38.466905160375646 40.0 38.0 41.0 34.0 41.0 22-23 38.50794491051156 40.0 38.0 41.0 34.0 41.0 24-25 38.45354468026137 40.0 38.0 41.0 34.0 41.0 26-27 38.33332402383516 40.0 38.0 41.0 34.0 41.0 28-29 38.19119229088273 40.0 38.0 41.0 34.0 41.0 30-31 37.96728933707135 40.0 38.0 41.0 33.0 41.0 32-33 37.7427736619373 40.0 37.5 41.0 33.0 41.0 34-35 37.433734423468465 40.0 37.0 41.0 32.5 41.0 36-37 37.15650190276194 40.0 37.0 41.0 31.0 41.0 38-39 36.94509449851297 40.0 36.5 41.0 31.0 41.0 40-41 36.73050665700184 40.0 36.0 41.0 30.5 41.0 42-43 36.49367217064097 40.0 36.0 41.0 30.0 41.0 44-45 36.19717026787903 39.0 35.0 41.0 29.5 41.0 46-47 36.026279860570725 39.0 35.0 41.0 28.5 41.0 48-49 35.859976548592385 39.0 35.0 41.0 28.0 41.0 50-51 34.69909797358519 38.0 34.0 40.0 25.5 40.5 52-53 34.80681753738901 38.5 34.5 39.5 26.0 40.5 54-55 35.30093230005011 39.0 35.0 40.5 27.0 41.0 56-57 35.17781603436697 39.0 35.0 41.0 26.0 41.0 58-59 34.940054577821364 38.5 35.0 41.0 26.0 41.0 60-61 34.6204611399516 38.0 35.0 40.0 26.0 41.0 62-63 34.22318491434906 37.0 34.0 40.0 24.5 41.0 64-65 33.82872648196906 37.0 34.0 40.0 23.5 41.0 66-67 33.435331037937985 36.0 34.0 39.0 22.5 41.0 68-69 33.01678971549178 35.5 33.0 39.0 21.5 41.0 70-71 32.582713114666724 35.0 33.0 38.5 20.0 40.0 72-73 32.155560008954176 35.0 33.0 37.5 18.5 40.0 74-75 31.730919188581296 35.0 32.0 37.0 17.5 39.0 76-77 31.316151410815365 35.0 32.0 36.5 14.0 39.0 78-79 30.903870761424567 35.0 31.0 36.0 8.5 38.5 80-81 30.48989137734381 35.0 31.0 35.5 6.5 37.0 82-83 30.118510622421677 34.5 31.0 35.0 2.0 37.0 84-85 29.759536941296865 34.0 30.5 35.0 2.0 36.5 86-87 29.377541652897847 34.0 30.0 35.0 2.0 36.0 88-89 28.961123322424875 34.0 29.5 35.0 2.0 36.0 90-91 28.560047116009848 34.0 29.0 35.0 2.0 35.0 92-93 28.15416891409323 34.0 29.0 35.0 2.0 35.0 94-95 27.678663269765806 34.0 28.0 35.0 2.0 35.0 96-97 27.08978989670721 34.0 26.5 35.0 2.0 35.0 98-99 26.373824178401254 33.5 25.0 35.0 2.0 35.0 100-101 23.431999658888614 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1402172784824316 1101 2 0.19675809211345552 1101 3 0.16441496834680436 1101 4 0.23740210844368903 1101 5 0.0885387046856394 1101 6 0.06876143679394886 1101 7 0.19517709611402978 1101 8 0.25857804542729923 1101 9 0.18658290435045188 1101 10-11 0.2387558683874289 1101 12-13 0.277210429935387 1101 14-15 0.3308324520440067 1101 16-17 0.34377364737971305 1101 18-19 0.38606560919226496 1101 20-21 0.3633778134545409 1101 22-23 0.2994062690454058 1101 24-25 0.3987827447280736 1101 26-27 0.3276799437330169 1101 28-29 0.3355240018234724 1101 30-31 0.47974443253769294 1101 32-33 0.2824057364381005 1101 34-35 0.3396183930587142 1101 36-37 0.3590839880126637 1101 38-39 0.10172432552881361 1101 40-41 0.18451732750505556 1101 42-43 0.180536830422227 1101 44-45 0.10196442088098934 1101 46-47 0.23669238020730177 1101 48-49 0.1886852888364885 1101 50-51 0.19948114128582972 1101 52-53 0.04102969923450672 1101 54-55 0.06968993964127179 1101 56-57 -0.018268749687592845 1101 58-59 0.0072562774733100355 1101 60-61 -0.1505954488777661 1101 62-63 -0.200257506898474 1101 64-65 -0.21945665392809843 1101 66-67 -0.1862716121993202 1101 68-69 -0.2225472645211326 1101 70-71 -0.14979584928625655 1101 72-73 -0.11339261599107431 1101 74-75 -0.180760779421977 1101 76-77 -0.27649675577443844 1101 78-79 -0.40607713494442876 1101 80-81 -0.278586057670811 1101 82-83 -0.35101210220944523 1101 84-85 -0.3707057806582199 1101 86-87 -0.3664351896227416 1101 88-89 -0.4168135766418821 1101 90-91 -0.37338321899010296 1101 92-93 -0.47602254889282136 1101 94-95 -0.44678887731429384 1101 96-97 -0.5391119638783906 1101 98-99 -0.6516768918159492 1101 100-101 -0.94429344692578 1102 1 0.220320633881272 1102 2 0.2121562353386821 1102 3 0.21219079210315783 1102 4 0.12056347490839414 1102 5 0.1862701247658407 1102 6 0.23946630620235254 1102 7 0.28602351197782383 1102 8 0.2935566518140291 1102 9 0.2879552311225524 1102 10-11 0.30993564290244535 1102 12-13 0.28380841696873205 1102 14-15 0.27399306870979245 1102 16-17 0.23601683151279929 1102 18-19 0.32989082035317097 1102 20-21 0.3507434582523956 1102 22-23 0.4904009892925103 1102 24-25 0.414115158827407 1102 26-27 0.3264865949362985 1102 28-29 0.3580082317020796 1102 30-31 0.48450249092852715 1102 32-33 0.41731744518114766 1102 34-35 0.353218966411454 1102 36-37 0.40031805965448797 1102 38-39 0.30963991375093514 1102 40-41 0.16839056474727698 1102 42-43 0.14405619178830875 1102 44-45 0.22996166902159132 1102 46-47 0.2903835818525735 1102 48-49 0.20530988886259394 1102 50-51 0.09279595361213921 1102 52-53 0.14333537959425513 1102 54-55 0.2198408245013681 1102 56-57 0.1496930242003245 1102 58-59 0.10506438153371533 1102 60-61 0.0927150301950519 1102 62-63 -0.09026736139853853 1102 64-65 0.006118889773496505 1102 66-67 -0.16137414963570507 1102 68-69 -0.15045507236228417 1102 70-71 -0.2570545844436509 1102 72-73 -0.38101240367139155 1102 74-75 -0.38655550784870485 1102 76-77 -0.4344550435842649 1102 78-79 -0.4767240166573181 1102 80-81 -0.39427735279751275 1102 82-83 -0.6561749059510404 1102 84-85 -0.6323201172030295 1102 86-87 -0.704725716254238 1102 88-89 -0.8152301863433031 1102 90-91 -0.7110410419245987 1102 92-93 -0.7289435999356257 1102 94-95 -0.9535945196972122 1102 96-97 -1.1886272237470088 1102 98-99 -1.1983842521147814 1102 100-101 -1.244108090790764 1103 1 0.19071769337471522 1103 2 0.2527832283233735 1103 3 0.3150952351303715 1103 4 0.25690498137705475 1103 5 0.3133750962187065 1103 6 0.24369333183403086 1103 7 0.23502730434298513 1103 8 0.344163278393232 1103 9 0.35118456861648184 1103 10-11 0.37799773101894374 1103 12-13 0.3640070700907003 1103 14-15 0.4073766537286261 1103 16-17 0.3832637604182878 1103 18-19 0.4293288935735262 1103 20-21 0.3839873919866008 1103 22-23 0.4500527769048759 1103 24-25 0.5364765894487604 1103 26-27 0.4885945463758006 1103 28-29 0.47218110509250977 1103 30-31 0.5132719367649017 1103 32-33 0.6077326598161079 1103 34-35 0.6580051666058111 1103 36-37 0.5589304582969774 1103 38-39 0.4858622302444999 1103 40-41 0.6290279307427866 1103 42-43 0.5401716273598538 1103 44-45 0.6428127190398101 1103 46-47 0.7768875165737867 1103 48-49 0.6610509185333839 1103 50-51 0.7518373184839788 1103 52-53 0.6617560151475814 1103 54-55 0.739876014916824 1103 56-57 0.7881639399103548 1103 58-59 0.7472861418896386 1103 60-61 0.6768925349022936 1103 62-63 0.5816202133858397 1103 64-65 0.5315135481949014 1103 66-67 0.42987180745248565 1103 68-69 0.2932983916349272 1103 70-71 0.30149100175957955 1103 72-73 0.4179865485880825 1103 74-75 0.3207251024981126 1103 76-77 0.3547716403928973 1103 78-79 0.249973247069601 1103 80-81 0.2817259726398085 1103 82-83 0.14710326915828986 1103 84-85 0.19518919288261216 1103 86-87 0.013609078601206193 1103 88-89 0.05138551685761783 1103 90-91 0.15368654553895666 1103 92-93 0.01287614693633543 1103 94-95 -0.047944313186036425 1103 96-97 -0.1107753101020883 1103 98-99 -0.34186586350933723 1103 100-101 -0.47119588461365325 1104 1 0.1730602110013848 1104 2 0.1960501753238546 1104 3 0.28602242800800326 1104 4 0.2140753401844151 1104 5 0.19389090047300783 1104 6 0.2616299654382388 1104 7 0.21943498090847413 1104 8 0.18524687064944345 1104 9 0.23399800223263867 1104 10-11 0.25647965247568294 1104 12-13 0.1918202422943338 1104 14-15 0.2946065585744648 1104 16-17 0.24982667120939084 1104 18-19 0.31392179958452004 1104 20-21 0.40180265549042105 1104 22-23 0.3703018843678265 1104 24-25 0.31424560160817805 1104 26-27 0.3653058647911891 1104 28-29 0.4135490768300869 1104 30-31 0.4356366184391405 1104 32-33 0.42200232924129466 1104 34-35 0.39533786259051595 1104 36-37 0.3341852421461695 1104 38-39 0.11897488783890964 1104 40-41 0.21910275074159102 1104 42-43 0.31354424103695777 1104 44-45 0.23934769603550876 1104 46-47 0.3585364507575832 1104 48-49 0.30166938322028614 1104 50-51 0.3222716553924414 1104 52-53 0.31968979188635416 1104 54-55 0.26390916202485215 1104 56-57 0.3128380145100138 1104 58-59 0.2842620097792192 1104 60-61 0.20593453269149364 1104 62-63 0.04444221759831635 1104 64-65 0.2403788871349093 1104 66-67 0.12339456527992354 1104 68-69 0.13637852204242762 1104 70-71 0.03378450453801918 1104 72-73 0.017797584677943235 1104 74-75 -0.12624783383673233 1104 76-77 -0.1926019106348278 1104 78-79 -0.13902726081758843 1104 80-81 -0.08058058209250518 1104 82-83 -0.12065224291150045 1104 84-85 -0.14054804645175878 1104 86-87 -0.11180451335625108 1104 88-89 -0.14291587684457951 1104 90-91 -0.05653393725262745 1104 92-93 -0.1132357899063976 1104 94-95 -0.13964782657036068 1104 96-97 -0.2471350846898055 1104 98-99 -0.18648599376746589 1104 100-101 -0.16501739961126205 1105 1 0.09771420902881545 1105 2 0.11027693613296208 1105 3 0.22814298303539715 1105 4 0.24205013820898813 1105 5 0.21010679430995083 1105 6 0.22460944566084606 1105 7 0.2380542843825566 1105 8 0.22056720064649227 1105 9 0.2714546963515829 1105 10-11 0.2852520264391032 1105 12-13 0.26997155740448164 1105 14-15 0.1925097171888197 1105 16-17 0.268346722766978 1105 18-19 0.25733841551253533 1105 20-21 0.20770240304339183 1105 22-23 0.18998118050706125 1105 24-25 0.292203664780466 1105 26-27 0.27598670623380883 1105 28-29 0.3212603721432501 1105 30-31 0.3356298532860862 1105 32-33 0.32689228868598263 1105 34-35 0.19059362098197852 1105 36-37 0.23758820314683504 1105 38-39 0.26069683292723056 1105 40-41 0.27548184499440964 1105 42-43 0.17585334800114794 1105 44-45 0.11093440927491827 1105 46-47 0.06587681985009652 1105 48-49 0.18751721712509806 1105 50-51 0.056506178324525536 1105 52-53 0.14634787749046296 1105 54-55 -0.09011663813252113 1105 56-57 0.027581330360774814 1105 58-59 0.09182812134726959 1105 60-61 0.23607640507222527 1105 62-63 0.04300462257462101 1105 64-65 0.152499548980515 1105 66-67 0.1715655705460648 1105 68-69 0.15063252149409578 1105 70-71 0.131626865790075 1105 72-73 0.05443978997541876 1105 74-75 0.04636059554396965 1105 76-77 0.06692938887986344 1105 78-79 -0.10264765027676859 1105 80-81 -0.045624324147897966 1105 82-83 -0.036772356423643515 1105 84-85 0.01844729271384793 1105 86-87 0.04185392118733233 1105 88-89 0.12263729903871834 1105 90-91 0.07496506043653639 1105 92-93 -0.07169107402502561 1105 94-95 0.03758583113426184 1105 96-97 -0.007280143813680695 1105 98-99 -0.21088282696371508 1105 100-101 -0.3157178599977293 1106 1 0.07145561092092834 1106 2 0.13583752894238188 1106 3 0.13758906642886615 1106 4 0.17500232027767026 1106 5 0.20400811909638605 1106 6 0.21571577449719825 1106 7 0.19303177397728177 1106 8 0.12886311579624277 1106 9 0.14967773847327948 1106 10-11 0.23875701170555885 1106 12-13 0.30606629319088796 1106 14-15 0.2885501466756253 1106 16-17 0.25661439048161583 1106 18-19 0.2762468159691167 1106 20-21 0.2895104301255813 1106 22-23 0.20748029020501946 1106 24-25 0.17998481111552422 1106 26-27 0.2230811980902061 1106 28-29 0.25577801967204294 1106 30-31 0.33619866917162256 1106 32-33 0.2307789776718252 1106 34-35 0.24090110453369817 1106 36-37 0.11955678207178977 1106 38-39 0.14413834114562718 1106 40-41 0.32159694086231383 1106 42-43 0.2263470725404204 1106 44-45 0.1390889895070586 1106 46-47 0.16227368887667382 1106 48-49 0.1695241916535295 1106 50-51 0.12849549130032756 1106 52-53 0.04504754780247566 1106 54-55 0.09246678040632617 1106 56-57 0.09504848267764032 1106 58-59 0.19235639555520123 1106 60-61 0.27671295615685665 1106 62-63 0.26012400464126273 1106 64-65 0.16099800253224572 1106 66-67 0.019276750614849902 1106 68-69 0.05920519521348666 1106 70-71 0.07533332279592031 1106 72-73 0.08343165784184947 1106 74-75 0.055164174090709395 1106 76-77 0.06667589715350886 1106 78-79 0.020596499865128237 1106 80-81 0.02165191256450072 1106 82-83 0.09719695854099086 1106 84-85 0.1038827162808893 1106 86-87 0.021225686671151323 1106 88-89 0.04411478395882895 1106 90-91 0.10317807338012486 1106 92-93 0.007942300019973203 1106 94-95 0.006144145280416069 1106 96-97 -0.1771765458035368 1106 98-99 -0.059607655214037436 1106 100-101 -0.2950400107974751 1107 1 0.15564858953502636 1107 2 0.16573910346350829 1107 3 0.10058955968194283 1107 4 0.09558079766068772 1107 5 0.15651726269852873 1107 6 0.1993319949506258 1107 7 0.15964324814903819 1107 8 0.21496975260848217 1107 9 0.2408290648328517 1107 10-11 0.24032672310134018 1107 12-13 0.30351887642478204 1107 14-15 0.230577457750492 1107 16-17 0.27580991722882686 1107 18-19 0.3651976400701642 1107 20-21 0.26884322858973775 1107 22-23 0.2857776665489311 1107 24-25 0.3479919254212902 1107 26-27 0.37408755716615616 1107 28-29 0.3166593667236768 1107 30-31 0.38713881772061853 1107 32-33 0.26736668973482836 1107 34-35 0.362377363915229 1107 36-37 0.47995173247018386 1107 38-39 0.4522691371251071 1107 40-41 0.6143871568498511 1107 42-43 0.53867328269898 1107 44-45 0.6085598988324108 1107 46-47 0.864019006554166 1107 48-49 0.8699954427766556 1107 50-51 0.6054293605944068 1107 52-53 0.5828968415909657 1107 54-55 0.622911854322652 1107 56-57 0.6451095764714552 1107 58-59 0.6713214032405332 1107 60-61 0.5686329181702305 1107 62-63 0.6176954393527652 1107 64-65 0.5445305132313365 1107 66-67 0.49607267278319966 1107 68-69 0.5311673395749494 1107 70-71 0.6243340248117093 1107 72-73 0.6165133843504194 1107 74-75 0.5935414124056244 1107 76-77 0.6130859971754106 1107 78-79 0.626169645612201 1107 80-81 0.6064299384469827 1107 82-83 0.5559974325551487 1107 84-85 0.6271812490064903 1107 86-87 0.47492713143192233 1107 88-89 0.574743753264567 1107 90-91 0.6227938885882836 1107 92-93 0.6022018370988889 1107 94-95 0.6681221105807786 1107 96-97 0.7386013102034035 1107 98-99 0.5953941561094958 1107 100-101 0.33634203929329587 1108 1 -0.003832372694375863 1108 2 0.08843508166361147 1108 3 0.010072281228886482 1108 4 0.13635825963010717 1108 5 0.128144944191682 1108 6 0.13697982649401297 1108 7 0.08165973881879296 1108 8 0.11406233571668167 1108 9 0.19534366912323975 1108 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12537.0 15 12928.0 16 16225.0 17 22904.0 18 19096.0 19 13577.0 20 13184.0 21 13703.0 22 14669.0 23 15839.0 24 17663.0 25 19958.0 26 22872.0 27 26781.0 28 31649.0 29 37608.0 30 45491.0 31 56127.0 32 70740.0 33 92940.0 34 131393.0 35 200971.0 36 330405.0 37 516308.0 38 451633.0 39 77207.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88075801874072 18.45091584656413 14.375816000239716 23.292510134455434 2 15.625939143501128 19.85430469230244 40.00469571750563 24.5150604466908 3 19.4944416672821 24.16962253027617 29.195110050334705 27.140825752107027 4 13.318421567212674 15.965139085876572 35.96640284311222 34.75003650379853 5 14.32592125155892 36.98784074747872 33.23772386853713 15.448514132425228 6 32.85455097833063 34.84931626090637 17.095568155440553 15.200564605322448 7 29.261587674408286 32.555160300287795 20.07961245258928 18.10363957271464 8 26.92210694490258 34.18867463987632 19.534946836497426 19.354271578723676 9 27.170592949333418 16.68160198819 20.083057456608625 36.06474760586796 10-11 25.541587262031456 26.027658842696468 26.356784477115674 22.073969418156402 12-13 26.547226579060958 23.46114808171194 27.09683902963179 22.894786309595315 14-15 23.430782376604547 24.70467126015961 25.627914270444297 26.236632092791545 16-17 22.681067615445766 27.75392222668373 26.464570346186882 23.10043981168362 18-19 22.764492542565065 26.095954555725758 28.91304890546974 22.226503996239437 20-21 23.3777251643943 25.300847224361895 29.053654697928007 22.2677729133158 22-23 23.052699371421355 25.187980353929728 29.043594199731633 22.71572607491729 24-25 22.822211010403944 25.43636453662039 29.101177838503716 22.640246614471955 26-27 22.359385601210207 25.71660045873527 29.020309717807592 22.903704222246933 28-29 22.43817261153878 26.03742797039618 29.014327918259355 22.510071499805687 30-31 22.557022007303544 25.79780236382566 28.645963257783407 22.99921237108738 32-33 22.196840823134064 25.803002976345436 28.75327231554895 23.24688388497155 34-35 23.029504776773365 25.87764212502506 28.461564296745024 22.631288801456552 36-37 23.16049517137819 25.480509866112026 28.530224405475202 22.828770557034577 38-39 22.718242297031317 25.79023667402757 28.485528372704007 23.005992656237108 40-41 23.03656083149686 25.714863518411523 28.629888393521824 22.618687256569793 42-43 22.782564122784535 25.783818163272127 28.28489176936284 23.148725944580498 44-45 22.642475875575276 26.003071569805346 28.41384238123385 22.94061017338553 46-47 23.13227258777682 26.299336155358848 27.669415827002634 22.898975429861693 48-49 22.949748759873593 26.439741483608042 27.458911603553904 23.15159815296446 50-51 23.114936761816665 26.607101562367724 27.43116004072928 22.84680163508633 52-53 23.45515310448173 26.293259013571475 27.401840219941686 22.849747662005107 54-55 22.849830774140003 26.56337027878281 27.580332725493218 23.006466221583974 56-57 22.838061371769225 26.742894873674146 27.355901287392737 23.063142467163892 58-59 22.974360468391822 26.169463960294276 27.448623574727684 23.407551996586225 60-61 22.61027337558327 26.528194968333963 27.687138378845255 23.174393277237513 62-63 22.774599246909244 26.49994075297505 27.832473840777855 22.892986159337855 64-65 22.575153455428964 26.4665203645251 27.66085680415339 23.297469375892554 66-67 22.45399675127443 26.629089255069545 27.56165097678263 23.355263016873394 68-69 22.91667202628873 26.278784386263244 27.709010253600162 23.095533333847857 70-71 22.773795890612412 26.32949703027011 27.322929881961116 23.573777197156364 72-73 22.69883577741839 26.604141815878567 27.202982844784255 23.494039561918786 74-75 22.682352154375245 26.99241019256607 27.100465488754615 23.22477216430407 76-77 22.85193409616241 26.548020557553563 27.31217830604627 23.287867040237757 78-79 22.866385349813566 26.769806657508443 27.214363270668347 23.149444722009644 80-81 22.926886021315536 26.887285455152398 26.927887905526347 23.25794061800572 82-83 23.01077665180207 26.80501953254746 27.059660521539374 23.124543294111092 84-85 22.845851597907867 26.867004326928328 26.956379942577612 23.330764132586186 86-87 22.934306394165063 26.906997663916883 26.890519513858145 23.26817642805991 88-89 22.932130785054497 26.876958318055387 26.693387263134728 23.49752363375539 90-91 22.781503285653596 26.86056645533942 26.85968765178116 23.498242607225823 92-93 22.939319642265367 26.64230009255897 26.945613995225003 23.47276626995066 94-95 22.750380087182663 26.428710905892494 27.154161389315373 23.666747617609467 96-97 23.09347396787066 26.03069321340758 27.057930080416586 23.817902738305172 98-99 22.78042223533555 26.382795752396465 26.87513142205035 23.961650590217634 100-101 23.11255414224033 25.769541995006012 27.331277221128353 23.7866266416253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2383.0 1 1402.0 2 509.5 3 620.5 4 571.5 5 570.5 6 946.5 7 1875.0 8 2982.0 9 3484.0 10 3146.0 11 2795.5 12 2921.5 13 3165.5 14 3399.0 15 3642.0 16 3957.5 17 4179.0 18 4291.0 19 4547.5 20 4998.0 21 5590.5 22 6475.5 23 7555.0 24 8894.5 25 10825.5 26 13392.0 27 16249.5 28 18517.0 29 20849.5 30 23736.5 31 28089.0 32 35246.0 33 38893.5 34 38927.0 35 41342.5 36 46938.5 37 51889.5 38 55119.5 39 58078.5 40 59996.0 41 62038.0 42 62926.0 43 65720.0 44 70198.0 45 75024.0 46 77815.0 47 80313.0 48 86027.5 49 86028.5 50 82509.5 51 81527.0 52 81984.0 53 84225.0 54 86319.0 55 83378.0 56 77961.0 57 72596.5 58 69429.5 59 63924.5 60 52947.0 61 44473.0 62 37666.0 63 28814.5 64 20826.5 65 15932.5 66 12079.0 67 9337.0 68 7378.0 69 5856.5 70 4461.5 71 3186.0 72 2488.0 73 2108.0 74 1621.5 75 1213.5 76 888.0 77 604.0 78 452.5 79 327.0 80 212.0 81 137.0 82 124.5 83 106.5 84 78.5 85 52.0 86 35.5 87 24.5 88 16.0 89 16.0 90 11.0 91 7.5 92 7.5 93 10.0 94 8.5 95 6.5 96 5.5 97 2.5 98 3.5 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39163850721130783 2 0.1157646757842897 3 0.11751287162486276 4 0.13017663173828228 5 0.13252177250002664 6 0.07210241869290382 7 0.08199464881517092 8 0.07862617390284721 9 0.07832770144226156 10-11 0.07645158883286608 12-13 0.08086471735723955 14-15 0.07429832322435535 16-17 0.07555617145110914 18-19 0.08568291564955069 20-21 0.08425451173103368 22-23 0.09427465862212321 24-25 0.10698105765848355 26-27 0.10785515557877008 28-29 0.10239737344234685 30-31 0.09237722655125732 32-33 0.09094882263274029 34-35 0.3400667299144024 36-37 0.5315581328415644 38-39 0.5117736725970301 40-41 0.4824807325366961 42-43 0.5123919369796719 44-45 0.5230090287919327 46-47 0.5201522209548987 48-49 0.5264841010116085 50-51 0.5248638219398578 52-53 0.5241602797113345 54-55 0.5180842331922696 56-57 0.4881943482107642 58-59 0.47770517316732575 60-61 0.428286661478931 62-63 0.5041839443135666 64-65 0.5300657705386361 66-67 0.540149875814137 68-69 0.5552014156122417 70-71 0.5503832173199305 72-73 0.5373570263615142 74-75 0.5403630704288409 76-77 0.5166984681966934 78-79 0.4898785856669261 80-81 0.4976815085650937 82-83 0.5112406860602701 84-85 0.5058895012311989 86-87 0.506635682382663 88-89 0.5167197876581637 90-91 0.5354595942906483 92-93 0.5418554327317692 94-95 0.5366748035944612 96-97 0.5376554988220997 98-99 0.538039249128567 100-101 0.5146091609725938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2345275.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.905196198501564 #Duplication Level Percentage of deduplicated Percentage of total 1 71.75891648742582 32.9410714034717 2 14.49925523831546 13.311823128940453 3 5.268686576345492 7.255802729866538 4 2.623862095241088 4.817956171194142 5 1.510787994588299 3.4676509652958294 6 0.875159775402882 2.4104628716943513 7 0.59717312317576 1.9189344568698645 8 0.43189922598590985 1.5861134965491326 9 0.3217995228962874 1.3295043211624442 >10 1.8802047473323023 15.92257577721686 >50 0.15029174299024986 4.755016385907507 >100 0.0742383946000978 5.979526063199098 >500 0.004560586859291906 1.4226288299621395 >1k 0.00297834243872111 2.1581044787883346 >5k 1.8614640242006937E-4 0.7228289198816306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9566 0.40788393685175517 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7352 0.3134813614608095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4815 0.20530641395998336 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2627 0.11201245056549872 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2545 0.10851605888435258 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2512 0.10710897442730598 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2368 0.10096896952382983 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.263892294080652E-5 0.0 5 0.0 0.0 0.0 4.263892294080652E-5 0.0 6 0.0 0.0 0.0 4.263892294080652E-5 0.0 7 0.0 0.0 0.0 4.263892294080652E-5 0.0 8 0.0 0.0 0.0 4.263892294080652E-5 0.0 9 0.0 0.0 0.0 4.263892294080652E-5 0.0 10-11 0.0 0.0 0.0 6.395838441120977E-5 0.0 12-13 0.0 0.0 0.0 8.527784588161304E-5 0.0 14-15 0.0 0.0 0.0 8.527784588161304E-5 0.0 16-17 0.0 0.0 0.0 1.7055569176322607E-4 0.0 18-19 0.0 0.0 0.0 1.7055569176322607E-4 0.0 20-21 0.0 0.0 0.0 1.9187515323362933E-4 0.0 22-23 0.0 0.0 0.0 3.411113835264521E-4 0.0 24-25 0.0 0.0 0.0 5.969449211712912E-4 0.0 26-27 0.0 0.0 0.0 0.0013218066111650019 0.0 28-29 0.0 0.0 0.0 0.0036669473729093608 0.0 30-31 0.0 0.0 0.0 0.011150078349020903 0.0 32-33 0.0 0.0 0.0 0.023536685463325196 0.0 34-35 0.0 0.0 0.0 0.042340450480220865 0.0 36-37 0.0 0.0 0.0 0.07564144929699076 0.0 38-39 0.0 0.0 0.0 0.12303461214569719 0.0 40-41 0.0 0.0 0.0 0.17558708467024123 0.0 42-43 0.0 0.0 0.0 0.22958928057477268 0.0 44-45 0.0 0.0 0.0 0.29919732227563933 0.0 46-47 0.0 0.0 0.0 0.38357975077549544 4.263892294080652E-5 48-49 0.0 0.0 0.0 0.47156516826384964 4.263892294080652E-5 50-51 0.0 0.0 0.0 0.5744955282429566 4.263892294080652E-5 52-53 0.0 0.0 0.0 0.6735031073115093 4.263892294080652E-5 54-55 0.0 0.0 0.0 0.7827866668087964 4.263892294080652E-5 56-57 0.0 0.0 0.0 0.9144343413885365 4.263892294080652E-5 58-59 0.0 0.0 0.0 1.0635213354510666 4.263892294080652E-5 60-61 0.0 0.0 0.0 1.2311988999157881 4.263892294080652E-5 62-63 0.0 0.0 0.0 1.4046327189775187 8.527784588161304E-5 64-65 0.0 0.0 0.0 1.5857841830915351 8.527784588161304E-5 66-67 0.0 0.0 0.0 1.8000660903305583 8.527784588161304E-5 68-69 0.0 0.0 0.0 2.0461779535448934 8.527784588161304E-5 70-71 0.0 0.0 0.0 2.3090469134749654 8.527784588161304E-5 72-73 0.0 0.0 0.0 2.5865410239737345 8.527784588161304E-5 74-75 0.0 0.0 0.0 2.883222649795866 8.527784588161304E-5 76-77 0.0 0.0 0.0 3.2041445033098466 8.527784588161304E-5 78-79 0.0 0.0 0.0 3.5666606261525833 8.527784588161304E-5 80-81 0.0 0.0 0.0 3.9635863598085512 8.527784588161304E-5 82-83 0.0 0.0 0.0 4.37537175810939 8.527784588161304E-5 84-85 0.0 0.0 0.0 4.803530502819498 8.527784588161304E-5 86-87 0.0 0.0 0.0 5.253669612305593 8.527784588161304E-5 88-89 0.0 0.0 0.0 5.748835424417178 8.527784588161304E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6845 0.0 39.33812 1 GTATCAA 11230 0.0 36.70658 1 ATCAACG 13960 0.0 29.1127 3 TATCAAC 14100 0.0 28.958014 2 TCAACGC 14125 0.0 28.839693 4 CAACGCA 14235 0.0 28.649498 5 AACGCAG 14675 0.0 27.838415 6 ACGCAGA 16525 0.0 24.549982 7 CGCAGAG 16760 0.0 24.148235 8 TACATGG 11265 0.0 22.748182 2 TAAGGTG 1085 0.0 22.700998 5 GTACATG 11445 0.0 22.697367 1 GCAGAGT 17805 0.0 22.305567 9 GAGTACT 8910 0.0 22.049366 12-13 ACATGGG 11360 0.0 21.557224 3 GTACTTT 9410 0.0 20.803644 14-15 AGTACTT 9230 0.0 20.541235 12-13 GTAAGGT 985 0.0 20.197315 4 GTATAGG 580 0.0 19.651117 1 AGAGTAC 15830 0.0 19.422308 10-11 >>END_MODULE