FastQCFastQC Report
Fri 27 May 2016
SRR939092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345275
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99420.4239161718774984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78480.33463026723944955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50460.2151560051593097No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26500.11299314579313728No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25440.10847341996141177No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24760.10557397320143692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.042.0722541
GTATCAA117150.034.937871
TATACCG804.5205912E-429.658795
ATCAACG146050.027.8778443
TCAACGC148700.027.4129414
TATCAAC148950.027.366932
CAACGCA150900.026.950395
AACGCAG154750.026.3105586
TAAGGTG12150.023.4341075
TACATGG109500.023.1419832
ACGCAGA176600.023.055267
GTAAGGT12150.023.043544
GTACATG111650.023.0025271
CGCAGAG177700.022.8591338
GAGTACT101200.022.11022412-13
ACATGGG110650.021.4862083
GTACTTT103650.021.38156714-15
GCAGAGT189950.021.3099869
GGACCGT1856.611992E-620.520686
TATAGGG4400.020.491532
Produced by FastQC (version 0.11.5)