##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939092_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2345275 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.516865186385395 34.0 31.0 34.0 31.0 34.0 2 32.733684535928624 34.0 31.0 34.0 31.0 34.0 3 32.8356184242786 34.0 31.0 34.0 31.0 34.0 4 36.2840118962595 37.0 37.0 37.0 35.0 37.0 5 36.24676082762149 37.0 37.0 37.0 35.0 37.0 6 36.28654891217448 37.0 37.0 37.0 35.0 37.0 7 36.239537794075325 37.0 37.0 37.0 35.0 37.0 8 36.215627591647035 37.0 37.0 37.0 35.0 37.0 9 37.96289091897539 39.0 38.0 39.0 35.0 39.0 10-11 37.88349020903732 39.0 38.0 39.0 35.0 39.0 12-13 37.797545064011686 39.0 38.0 39.0 35.0 39.0 14-15 39.21931500570295 40.5 39.0 41.0 36.0 41.0 16-17 39.30566202257731 41.0 39.0 41.0 36.0 41.0 18-19 39.34313587958768 41.0 39.0 41.0 36.0 41.0 20-21 39.304898359467444 41.0 39.0 41.0 36.0 41.0 22-23 39.2329592478494 41.0 39.0 41.0 36.0 41.0 24-25 39.15307927641748 41.0 39.0 41.0 35.5 41.0 26-27 39.00168381106693 40.5 39.0 41.0 35.0 41.0 28-29 38.841778043086634 40.0 38.0 41.0 35.0 41.0 30-31 38.631140484591356 40.0 38.0 41.0 35.0 41.0 32-33 38.5086921576361 40.0 38.0 41.0 34.0 41.0 34-35 38.39232627303834 40.0 38.0 41.0 34.0 41.0 36-37 38.22929315325494 40.0 38.0 41.0 34.0 41.0 38-39 38.07263071494814 40.0 38.0 41.0 33.5 41.0 40-41 37.853241517519265 40.0 37.5 41.0 33.0 41.0 42-43 37.634430717080086 40.0 37.0 41.0 33.0 41.0 44-45 37.34950229717197 40.0 37.0 41.0 32.5 41.0 46-47 36.99948300305934 40.0 36.5 41.0 31.5 41.0 48-49 36.59938535992581 40.0 36.0 41.0 30.5 41.0 50-51 36.30011896259501 39.0 35.0 41.0 30.0 41.0 52-53 35.99407638763044 39.0 35.0 40.5 29.5 41.0 54-55 35.708124420377146 39.0 35.0 40.0 29.0 41.0 56-57 35.42465467802283 38.0 35.0 40.0 28.0 41.0 58-59 35.052925563100274 38.0 34.5 40.0 27.5 41.0 60-61 34.644610759932206 37.5 34.0 40.0 26.5 41.0 62-63 34.214796345844306 37.0 34.0 40.0 26.0 41.0 64-65 33.740351344725035 36.0 33.5 39.0 25.5 41.0 66-67 33.29917451045186 35.5 33.0 39.0 24.5 40.0 68-69 32.797661468271315 35.0 32.5 38.5 23.0 40.0 70-71 32.28327850678492 35.0 32.0 37.5 22.0 40.0 72-73 31.717520546630993 35.0 31.0 37.0 20.0 39.0 74-75 31.196066772553326 35.0 31.0 36.0 18.0 39.0 76-77 29.237312255492427 32.5 28.5 34.5 13.0 36.5 78-79 30.224200999882743 34.0 30.0 35.0 14.0 37.0 80-81 30.263516858364156 34.0 30.0 35.0 12.0 37.0 82-83 29.89885962200595 34.0 30.0 35.0 7.0 36.5 84-85 29.43920052019486 34.0 29.5 35.0 3.5 36.0 86-87 28.760335994712776 34.0 29.0 35.0 2.0 35.5 88-89 28.073736555414612 33.0 27.5 35.0 2.0 35.0 90-91 27.50703563547985 33.0 26.5 35.0 2.0 35.0 92-93 27.048596433254097 33.0 26.0 34.5 2.0 35.0 94-95 26.51147093624415 32.0 25.0 34.0 2.0 35.0 96-97 25.70777627357133 32.0 23.5 34.0 2.0 35.0 98-99 24.4666608393472 32.0 10.5 34.0 2.0 35.0 100-101 18.55203782072465 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17204292292998957 1101 2 0.18069476185488753 1101 3 0.15427382447457205 1101 4 0.14188877707567826 1101 5 0.09997588674465163 1101 6 0.06855604891440947 1101 7 0.13641958479354344 1101 8 0.20370994652772367 1101 9 0.18387953073903418 1101 10-11 0.21340596312730753 1101 12-13 0.2478729455978126 1101 14-15 0.3310687583730143 1101 16-17 0.3145792509467995 1101 18-19 0.30275951963850645 1101 20-21 0.29171678298560266 1101 22-23 0.3203351288422027 1101 24-25 0.3296276069888364 1101 26-27 0.40228817868665345 1101 28-29 0.2958921489765416 1101 30-31 0.2905416992739944 1101 32-33 0.4320464855742401 1101 34-35 0.38973165158923706 1101 36-37 0.48815162819054336 1101 38-39 0.5070404434582727 1101 40-41 0.5953376382897844 1101 42-43 0.6473897821932297 1101 44-45 0.6427225147174198 1101 46-47 0.6296466990163978 1101 48-49 0.6929161753338917 1101 50-51 0.5939176389055163 1101 52-53 0.7206923219877126 1101 54-55 0.7138540172885683 1101 56-57 0.5459200402123514 1101 58-59 0.41422310832535203 1101 60-61 0.6054424078880345 1101 62-63 0.4024496569347704 1101 64-65 0.5423088844400397 1101 66-67 0.42681848548473766 1101 68-69 0.5787421228267036 1101 70-71 0.4260041007048869 1101 72-73 0.40419134975239857 1101 74-75 0.19912261059458913 1101 76-77 0.4663109779236265 1101 78-79 0.40590796476322666 1101 80-81 0.45135493754921185 1101 82-83 0.5252330166075119 1101 84-85 0.5467707690671055 1101 86-87 0.6884219898470967 1101 88-89 0.6482843099044366 1101 90-91 0.7526343104780331 1101 92-93 0.5800236801061693 1101 94-95 0.2686330801117265 1101 96-97 0.15023298565579424 1101 98-99 -0.006037482321907817 1101 100-101 -0.830204725629315 1102 1 0.05213693018648513 1102 2 0.060404132004322264 1102 3 0.1190469080567027 1102 4 0.07561239535208131 1102 5 0.10857520394075948 1102 6 0.0736030720179528 1102 7 0.14420386082861114 1102 8 0.1288683293769921 1102 9 0.15584971016493654 1102 10-11 0.15144433335658647 1102 12-13 0.20511103694084198 1102 14-15 0.1804955030187685 1102 16-17 0.2166783957865306 1102 18-19 0.15784602890582278 1102 20-21 0.2194137707414825 1102 22-23 0.17807514726582951 1102 24-25 0.17123648071295605 1102 26-27 0.21611919348592323 1102 28-29 0.2213268375957611 1102 30-31 0.18911386790668416 1102 32-33 0.2822359315215337 1102 34-35 0.33444639692051936 1102 36-37 0.3817814534386983 1102 38-39 0.4514304382194183 1102 40-41 0.3882426903764653 1102 42-43 0.44286265213135323 1102 44-45 0.5493706019511677 1102 46-47 0.5065720755225627 1102 48-49 0.31965799716998333 1102 50-51 0.19510787165479115 1102 52-53 0.38803818962190917 1102 54-55 0.28180916957564506 1102 56-57 0.2847185799546139 1102 58-59 0.0770146778589762 1102 60-61 0.07490246502855058 1102 62-63 0.15066060750452692 1102 64-65 0.22810077921111116 1102 66-67 0.322725459783193 1102 68-69 0.3261986490959501 1102 70-71 0.1463261831594025 1102 72-73 0.2623430272570637 1102 74-75 0.2078665318676478 1102 76-77 0.06731230029626545 1102 78-79 0.27096485210447696 1102 80-81 0.42221405130636214 1102 82-83 0.27119067536525776 1102 84-85 0.2135822832519345 1102 86-87 0.38195263714753125 1102 88-89 0.27001805923010735 1102 90-91 0.44742914796642097 1102 92-93 0.3760301731700473 1102 94-95 0.3668014771584929 1102 96-97 0.551404132614433 1102 98-99 0.48355637984362687 1102 100-101 -0.21886810681344926 1103 1 0.18585766178269836 1103 2 0.14979898592803664 1103 3 0.15846482366044512 1103 4 0.10527844704061096 1103 5 0.18459238536689782 1103 6 0.13253752749657366 1103 7 0.13978275644935678 1103 8 0.15992804679074624 1103 9 0.18340984136027316 1103 10-11 0.22204629065736725 1103 12-13 0.1398272643916485 1103 14-15 0.03606224143288017 1103 16-17 0.18537406322761285 1103 18-19 0.21950707725373775 1103 20-21 0.2760320932937219 1103 22-23 0.20091024391609835 1103 24-25 0.2014664996178439 1103 26-27 0.18898755399977318 1103 28-29 0.21710811606697433 1103 30-31 0.24671136281983053 1103 32-33 0.09969134560680715 1103 34-35 0.25045015891638656 1103 36-37 0.13756324439169276 1103 38-39 0.17944971512660857 1103 40-41 0.1729391303363812 1103 42-43 0.20360768675234908 1103 44-45 0.2306434738231289 1103 46-47 0.20470061544299512 1103 48-49 0.14624911073594404 1103 50-51 0.10705377445787434 1103 52-53 0.04816905279307093 1103 54-55 0.11289232828556095 1103 56-57 0.2111029450845905 1103 58-59 0.07821711195762049 1103 60-61 0.21353163321368385 1103 62-63 0.20236100306453153 1103 64-65 0.10494832347435334 1103 66-67 0.022595488709939104 1103 68-69 -0.057481985546296244 1103 70-71 -0.2248591492324863 1103 72-73 -0.30929812920551925 1103 74-75 -0.24642033075686598 1103 76-77 -0.16089607289676167 1103 78-79 -0.11938872236102327 1103 80-81 6.434559923462757E-4 1103 82-83 -0.042595511542540265 1103 84-85 -0.1017494158339467 1103 86-87 -0.10943055117244072 1103 88-89 0.06842243772257461 1103 90-91 0.2383415648304883 1103 92-93 0.17850237577876626 1103 94-95 0.12650849891808846 1103 96-97 -0.13034183586224302 1103 98-99 -0.23463677074249745 1103 100-101 -0.8529743860438934 1104 1 0.06507947404369219 1104 2 0.09985238877220581 1104 3 0.18497672166474644 1104 4 0.04751934988478013 1104 5 0.08155663907148636 1104 6 0.030308425560626517 1104 7 0.06417758645653038 1104 8 0.07567759118606432 1104 9 0.15732834040424848 1104 10-11 0.13064340167553468 1104 12-13 0.1037649399114855 1104 14-15 0.12060912016734449 1104 16-17 0.10275790270539176 1104 18-19 0.1566709754372937 1104 20-21 0.2389908722731633 1104 22-23 0.16468827107312478 1104 24-25 0.15828348470986242 1104 26-27 0.10676117822300313 1104 28-29 0.11094242160593382 1104 30-31 0.17010675673116538 1104 32-33 0.2692313715986785 1104 34-35 0.2532239325182033 1104 36-37 0.28089246358982933 1104 38-39 0.4171838660587355 1104 40-41 0.33030853610836886 1104 42-43 0.387212640146231 1104 44-45 0.3990650276591694 1104 46-47 0.29700771167267703 1104 48-49 0.24899870367929822 1104 50-51 0.3167322930704515 1104 52-53 0.25686073912791585 1104 54-55 0.2625939445618499 1104 56-57 0.2968602571663297 1104 58-59 0.18290722608286103 1104 60-61 0.23685149757792345 1104 62-63 0.39363374595411216 1104 64-65 0.3015879588412247 1104 66-67 0.17070108816619722 1104 68-69 0.24438459323597073 1104 70-71 0.18616256294485822 1104 72-73 0.08129699507746224 1104 74-75 -0.11264480569114 1104 76-77 -0.03552682770675375 1104 78-79 0.29740367128142253 1104 80-81 0.4797094477167718 1104 82-83 0.2829765150767223 1104 84-85 0.0019272579530991152 1104 86-87 0.045862492266852684 1104 88-89 0.20842658297831207 1104 90-91 0.3650766904578795 1104 92-93 0.05878617641810635 1104 94-95 0.22115523723668318 1104 96-97 -0.2274995348345179 1104 98-99 -0.4204959403469424 1104 100-101 -0.6382711452754926 1105 1 -0.013850278743369415 1105 2 0.10894884917291847 1105 3 0.0973751108462011 1105 4 0.0989808009745019 1105 5 0.006085125706746908 1105 6 0.06276513748260726 1105 7 0.05737415687998748 1105 8 -0.04175211353280872 1105 9 0.0017583050474243578 1105 10-11 0.08611133663036696 1105 12-13 0.09492039244681649 1105 14-15 0.07909600467193911 1105 16-17 0.11495654184143689 1105 18-19 0.1664417257639812 1105 20-21 0.1290557999394366 1105 22-23 0.1338961428627954 1105 24-25 0.0638343818047602 1105 26-27 0.16355419302444574 1105 28-29 0.13997650017428498 1105 30-31 0.13621022440619157 1105 32-33 0.12262783449181569 1105 34-35 0.2197902790863111 1105 36-37 0.2427823981102648 1105 38-39 0.18124826319457554 1105 40-41 0.26532612550630574 1105 42-43 0.19702816510429955 1105 44-45 0.23013336994743128 1105 46-47 0.21751004208987723 1105 48-49 0.21992456828366613 1105 50-51 0.1578700315865973 1105 52-53 0.29212009373231496 1105 54-55 0.21475852649319194 1105 56-57 0.23694548029175877 1105 58-59 0.11232042026767886 1105 60-61 -0.06579252006844172 1105 62-63 -0.10559716296200605 1105 64-65 0.014155743894512796 1105 66-67 -0.05274212549513635 1105 68-69 -0.07091426630164932 1105 70-71 -0.08822314335492365 1105 72-73 -0.20181442343270461 1105 74-75 -0.09617655384229451 1105 76-77 -0.3429126835520222 1105 78-79 -0.4534312910295526 1105 80-81 -0.07318203210704866 1105 82-83 -0.08369433530301507 1105 84-85 -0.25164403073734576 1105 86-87 -0.09026573942896121 1105 88-89 -0.004278730515320461 1105 90-91 0.07331477755744586 1105 92-93 0.11827056493728705 1105 94-95 -0.07659695164639047 1105 96-97 -0.3394516909600078 1105 98-99 -0.2602815384042785 1105 100-101 -0.7575799602699185 1106 1 -0.03683558259772468 1106 2 0.029608701098048584 1106 3 0.06273428716170315 1106 4 0.06513508713075566 1106 5 0.05426831459441672 1106 6 0.03700778579757724 1106 7 0.038098798995569894 1106 8 0.08687060833513272 1106 9 0.15849969759030813 1106 10-11 0.01890925634084084 1106 12-13 0.11498119221428027 1106 14-15 0.10730104951851871 1106 16-17 0.0032278855048275545 1106 18-19 9.549749032089494E-4 1106 20-21 0.03294981957452592 1106 22-23 0.0065819950072096844 1106 24-25 0.10808388055833973 1106 26-27 0.049356276543697675 1106 28-29 0.0168819377736682 1106 30-31 0.11676626158894265 1106 32-33 0.145139833383503 1106 34-35 -0.04274523737811364 1106 36-37 -0.0022152305840634767 1106 38-39 0.008970250127745771 1106 40-41 0.05025751912859988 1106 42-43 -0.13512801469676106 1106 44-45 0.06831948684514799 1106 46-47 0.05059105235108774 1106 48-49 0.07549122959576948 1106 50-51 0.2109922719689976 1106 52-53 0.2495130853766483 1106 54-55 0.20888561402836814 1106 56-57 0.19584231744595115 1106 58-59 0.01633353639280699 1106 60-61 0.042001171238560175 1106 62-63 0.07734428873602184 1106 64-65 0.09933576339191319 1106 66-67 -0.05050128413208199 1106 68-69 -0.1908042725656358 1106 70-71 -0.21577123453884894 1106 72-73 -0.24544315430820873 1106 74-75 -0.2388713600029284 1106 76-77 -0.2830387027247525 1106 78-79 -0.11892250402464555 1106 80-81 0.04807370598521388 1106 82-83 0.04649130223398856 1106 84-85 -0.22736662027113042 1106 86-87 -0.1499543291990264 1106 88-89 -0.003969993316331966 1106 90-91 0.2328290678613527 1106 92-93 0.2250220291547933 1106 94-95 -0.02827097446022009 1106 96-97 -0.3698786662193889 1106 98-99 -0.6420564394511352 1106 100-101 -1.3964397744581447 1107 1 0.1082835656504102 1107 2 0.11960262241906605 1107 3 0.1251815592265899 1107 4 0.06368213958545255 1107 5 0.03128783800142543 1107 6 0.08005977385307261 1107 7 0.10300926639890662 1107 8 0.13465434653850394 1107 9 0.12229717319883804 1107 10-11 0.1982172898165615 1107 12-13 0.13307518864893808 1107 14-15 0.1131180587675189 1107 16-17 0.09215547963736981 1107 18-19 0.213519829495894 1107 20-21 0.18608893536210758 1107 22-23 0.21153703938975355 1107 24-25 0.22654116504665467 1107 26-27 0.1874771665278061 1107 28-29 0.19316854032425823 1107 30-31 0.203685026286756 1107 32-33 0.16333125241734336 1107 34-35 0.11602392222916791 1107 36-37 0.24767066626669276 1107 38-39 0.16788073390463865 1107 40-41 0.22456425295409588 1107 42-43 0.3090957052501011 1107 44-45 0.27222007486012245 1107 46-47 0.2092192971274116 1107 48-49 0.2564436787712552 1107 50-51 0.20190536323310226 1107 52-53 0.20377552876000493 1107 54-55 0.2293414944851122 1107 56-57 0.15559522016867078 1107 58-59 0.17044227311232873 1107 60-61 0.1724380583818501 1107 62-63 0.1422402246172183 1107 64-65 0.11900622531010896 1107 66-67 0.1700610243741778 1107 68-69 -0.004079125050218124 1107 70-71 0.0975934677798449 1107 72-73 -0.14312660454184467 1107 74-75 -0.25395978738523084 1107 76-77 -0.1359716141663796 1107 78-79 0.14161008649547924 1107 80-81 0.20516345143797565 1107 82-83 0.14177664197483608 1107 84-85 0.021316946624011734 1107 86-87 -0.04151478954127441 1107 88-89 -0.029745741229220357 1107 90-91 0.20757962712028544 1107 92-93 0.32949886365713255 1107 94-95 0.3135567840217881 1107 96-97 0.18060179502340645 1107 98-99 0.263011377730038 1107 100-101 -0.49493351055342316 1108 1 -0.02427866532061529 1108 2 -0.020983660377545732 1108 3 0.04207296241916225 1108 4 -0.02492960249077214 1108 5 -0.012577030773030629 1108 6 0.027279074739382736 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0.6506507251095606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 16.0 5 53.0 6 100.0 7 242.0 8 561.0 9 1125.0 10 2015.0 11 3201.0 12 4992.0 13 6749.0 14 8535.0 15 11791.0 16 15473.0 17 22648.0 18 22620.0 19 15830.0 20 14649.0 21 15066.0 22 15625.0 23 16872.0 24 19218.0 25 21794.0 26 25654.0 27 30388.0 28 36676.0 29 45160.0 30 54666.0 31 67635.0 32 85009.0 33 112642.0 34 159309.0 35 243034.0 36 392633.0 37 551590.0 38 308364.0 39 13332.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.326006497802595 18.847010449840297 14.779225016925377 23.047758035431738 2 15.948705375702211 20.207353082261143 39.50479154896547 24.339149993071175 3 19.51984308876358 24.271993689439405 29.42563238852587 26.78253083327115 4 13.588299879545044 16.40118962595005 35.680293355789836 34.33021713871508 5 14.24681540544286 37.05036722772383 33.280660050527125 15.42215731630619 6 32.84429331315091 35.20124505654987 16.819562737845242 15.134898892453977 7 29.240536824039825 32.65075524192259 19.96767969641087 18.141028237626717 8 26.895822451524875 34.4659794693586 19.304516527912504 19.333681551204016 9 26.98809308076878 16.77274520045624 19.994456940017695 36.24470477875729 10-11 25.525066356823828 26.206585581648206 26.218737674686338 22.04961038684163 12-13 26.53622709490358 23.572459519672535 27.083348434618543 22.807964950805342 14-15 23.308687773158514 24.833749067263618 25.646476921436946 26.211086238140922 16-17 22.606050876449032 27.888324836300736 26.510826974150305 22.994797313099927 18-19 22.652801910223747 26.176418543667584 29.04196735990449 22.12881218620418 20-21 23.24200786688128 25.33278613382226 29.295818187632584 22.12938781166388 22-23 22.999925381884854 25.1831448337615 29.27191907132426 22.54501071302939 24-25 22.78289326411615 25.45776081696176 29.267719137414588 22.4916267815075 26-27 22.31212707000333 25.75505965280933 29.219546941822124 22.71326633536522 28-29 22.4139269015049 26.07394701002953 29.11288830217706 22.399237786288513 30-31 22.549061410708767 25.73881527752609 28.85864131072049 22.853482001044654 32-33 22.1683313268154 25.76053496532494 28.94890510637672 23.122228601482938 34-35 22.94275367031308 25.863652306665575 28.728026362501453 22.465567660519895 36-37 23.12445674287123 25.436908814690785 28.735924362400596 22.702710080037395 38-39 22.69451658377781 25.741804281078206 28.747340132330752 22.816339002813237 40-41 22.96185330431069 25.73723420518531 28.765529150465092 22.5353833400389 42-43 22.771588189164746 25.79118208562911 28.4200408772969 23.01718884790925 44-45 22.662603597705004 25.969372624259147 28.5593339045736 22.80868987346225 46-47 23.146893790053287 26.28150268830616 27.77287405491402 22.798729466726535 48-49 22.99597562005214 26.396849573129185 27.52255025784515 23.084624548973522 50-51 23.085852704666987 26.654474704521157 27.46617540648994 22.793497184321915 52-53 23.49605056976261 26.269605909754716 27.45383803605121 22.780505484431462 54-55 22.887337305859653 26.54989286970611 27.597637803669077 22.965132020765154 56-57 22.902216501021417 26.690027131169803 27.359842542851087 23.047913824957693 58-59 22.9437530314094 26.23118127694956 27.463743011102288 23.361322680538752 60-61 22.621414386055054 26.53464649942596 27.773100420109394 23.07083869440959 62-63 22.790479816855267 26.504499095831612 27.83331322175172 22.871707865561397 64-65 22.517371534221155 26.460355890459557 27.713268202161334 23.30900437315795 66-67 22.444074284351036 26.628394680866258 27.594653235725342 23.332877799057364 68-69 22.85914574982427 26.31762302203307 27.72753642098611 23.09569480715655 70-71 22.725786177466837 26.38406120910496 27.31073410674753 23.579418506680675 72-73 22.607796527059726 26.608990416902067 27.233629318523416 23.54958373751479 74-75 22.58148831160525 26.966325910607498 27.071430855656587 23.380754922130667 76-77 22.747221018276008 26.449179563117976 27.425560379165166 23.37803903944085 78-79 22.68515902514247 26.68763845227284 27.287515674998875 23.33968684758581 80-81 22.8328556696789 26.806258299952308 26.98777262058795 23.37311340978084 82-83 22.86009103410048 26.6686849090192 27.139034867979234 23.33218918890109 84-85 22.64116148426091 26.782680069501446 27.010862265619167 23.56529618061848 86-87 22.663351845283294 26.762658191377792 26.999211630814436 23.574778332524478 88-89 22.63466511163017 26.721688070896306 26.796250187047516 23.84739663042601 90-91 22.517312264743524 26.697817967418302 26.940230350638387 23.844639417199783 92-93 22.541727515962947 26.479559966315254 27.006896845785676 23.971815671936128 94-95 22.32096925114736 26.19629386666678 27.231886928374482 24.250849953811375 96-97 22.521559305411945 25.81381714297897 27.22171173956146 24.442911812047626 98-99 22.204498624360284 26.191434044444726 26.988953309263636 24.615114021931355 100-101 22.57741697599488 25.108127186181722 27.803296037706527 24.511159800116875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 736.0 1 528.5 2 392.0 3 523.0 4 483.0 5 461.0 6 811.0 7 1687.5 8 2763.0 9 3203.0 10 2820.0 11 2416.5 12 2447.0 13 2666.5 14 2904.5 15 3077.0 16 3262.5 17 3487.0 18 3714.5 19 3952.5 20 4434.0 21 5139.0 22 6020.5 23 7238.0 24 8732.0 25 10786.0 26 13458.5 27 16107.0 28 18392.0 29 21038.5 30 24016.5 31 28429.0 32 35683.5 33 39149.0 34 39129.5 35 41824.5 36 47521.0 37 52378.0 38 55534.0 39 58169.5 40 60421.0 41 62807.5 42 63387.0 43 66085.5 44 70673.5 45 75373.5 46 78142.0 47 81377.5 48 86927.0 49 86550.5 50 83564.0 51 82665.0 52 82615.5 53 84954.0 54 87350.5 55 83867.5 56 78387.0 57 73146.5 58 69533.5 59 64413.5 60 53398.5 61 44034.0 62 37093.5 63 28410.0 64 20337.5 65 15347.0 66 11537.5 67 8897.0 68 6943.5 69 5449.0 70 4200.0 71 2955.0 72 2264.0 73 2021.5 74 1563.5 75 1108.5 76 833.0 77 554.0 78 362.5 79 225.0 80 136.0 81 84.0 82 46.0 83 25.0 84 16.0 85 12.5 86 10.5 87 10.0 88 5.0 89 1.0 90 1.5 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4274125635586445 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010659730735201628 16-17 0.00189743207086589 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.003389794373794118 28-29 4.263892294080652E-5 30-31 0.0 32-33 6.395838441120977E-5 34-35 0.011832301116073808 36-37 0.026478771146240847 38-39 0.01997633539776785 40-41 0.008932854356098965 42-43 0.008868895971687756 44-45 0.003645627911438957 46-47 0.005031392907015169 48-49 0.0029420856829156495 50-51 5.969449211712912E-4 52-53 0.0 54-55 0.0 56-57 8.527784588161304E-5 58-59 0.0015989596102802444 60-61 0.002238543454392342 62-63 0.0011086119964609695 64-65 0.0025156964535075843 66-67 0.0018547931479250836 68-69 0.004072017140847022 70-71 1.2791676882241955E-4 72-73 0.0 74-75 0.0 76-77 0.0974512583812133 78-79 0.016138832333095265 80-81 2.3451407617443583E-4 82-83 0.0 84-85 0.0 86-87 0.10506230612614725 88-89 0.4109752587649636 90-91 0.4175203334363774 92-93 0.0 94-95 2.131946147040326E-5 96-97 0.0 98-99 1.0659730735201628E-4 100-101 1.9187515323362933E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2345275.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.60420272744065 #Duplication Level Percentage of deduplicated Percentage of total 1 70.65898513638314 31.516876975384488 2 14.830056011999107 13.229656496370165 3 5.643382892957584 7.551557838781518 4 2.7162145092207766 4.84618330481997 5 1.5488105271544423 3.4541729369795493 6 0.9506574556922264 2.5441990726829395 7 0.6690900372697518 2.089095936570358 8 0.4414657397888034 1.5752981883807482 9 0.3496511150948115 1.403631829940819 >10 1.9417456113487357 16.141660965364135 >50 0.16074453098231153 4.896132523080709 >100 0.08115390484640869 6.352003089671969 >500 0.004978707352657465 1.5375873230606616 >1k 0.0027765867928281206 1.8867089863763884 >5k 2.872331164994607E-4 0.9752345325356212 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9942 0.4239161718774984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7848 0.33463026723944955 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5046 0.2151560051593097 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2650 0.11299314579313728 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2544 0.10847341996141177 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2476 0.10557397320143692 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.263892294080652E-5 7 0.0 0.0 0.0 0.0 4.263892294080652E-5 8 0.0 0.0 0.0 0.0 4.263892294080652E-5 9 0.0 0.0 0.0 0.0 4.263892294080652E-5 10-11 0.0 0.0 0.0 0.0 4.263892294080652E-5 12-13 0.0 0.0 0.0 0.0 4.263892294080652E-5 14-15 0.0 0.0 0.0 0.0 4.263892294080652E-5 16-17 0.0 0.0 0.0 0.0 4.263892294080652E-5 18-19 0.0 0.0 0.0 0.0 4.263892294080652E-5 20-21 0.0 0.0 0.0 2.131946147040326E-5 4.263892294080652E-5 22-23 0.0 0.0 0.0 1.7055569176322604E-4 4.263892294080652E-5 24-25 0.0 0.0 0.0 3.8375030646725867E-4 4.263892294080652E-5 26-27 0.0 0.0 0.0 0.0011086119964609695 4.263892294080652E-5 28-29 0.0 0.0 0.0 0.003496391681146134 4.263892294080652E-5 30-31 0.0 0.0 0.0 0.011022161580198486 4.263892294080652E-5 32-33 0.0 0.0 0.0 0.023579324386266004 4.263892294080652E-5 34-35 0.0 0.0 0.0 0.042617603479336116 4.263892294080652E-5 36-37 0.0 0.0 0.0 0.07596124121904681 4.263892294080652E-5 38-39 0.0 0.0 0.0 0.1238021127586317 4.263892294080652E-5 40-41 0.0 0.0 0.0 0.17673833558964303 4.263892294080652E-5 42-43 0.0 0.0 0.0 0.23110296233917133 4.263892294080652E-5 44-45 0.0 0.0 0.0 0.30141454626856123 4.263892294080652E-5 46-47 0.0 0.0 0.0 0.3864578780739999 4.263892294080652E-5 48-49 0.0 0.0 0.0 0.47497628209911413 4.263892294080652E-5 50-51 0.0 0.0 0.0 0.5782051145388067 4.263892294080652E-5 52-53 0.0 0.0 0.0 0.6778096385285308 4.263892294080652E-5 54-55 0.0 0.0 0.0 0.7877967402543411 4.263892294080652E-5 56-57 0.0 0.0 0.0 0.9206596241378943 4.263892294080652E-5 58-59 0.0 0.0 0.0 1.0705354382748293 4.263892294080652E-5 60-61 0.0 0.0 0.0 1.239961198580124 4.263892294080652E-5 62-63 0.0 0.0 0.0 1.414546268561256 8.527784588161304E-5 64-65 0.0 0.0 0.0 1.5972327339011416 8.527784588161304E-5 66-67 0.0 0.0 0.0 1.8131136007504451 8.527784588161304E-5 68-69 0.0 0.0 0.0 2.061634563110936 8.527784588161304E-5 70-71 0.0 0.0 0.0 2.3275948449542163 8.527784588161304E-5 72-73 0.0 0.0 0.0 2.6071782626770847 8.527784588161304E-5 74-75 0.0 0.0 0.0 2.906098431953609 8.527784588161304E-5 76-77 0.0 0.0 0.0 3.22919487053757 8.527784588161304E-5 78-79 0.0 0.0 0.0 3.592755646992357 8.527784588161304E-5 80-81 4.263892294080652E-5 0.0 0.0 3.990619436953023 8.527784588161304E-5 82-83 4.263892294080652E-5 0.0 0.0 4.401722612486808 8.527784588161304E-5 84-85 4.263892294080652E-5 0.0 0.0 4.830520941041029 8.527784588161304E-5 86-87 4.263892294080652E-5 0.0 0.0 5.280425536450949 8.527784588161304E-5 88-89 4.263892294080652E-5 0.0 0.0 5.775889821023121 8.527784588161304E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7520 0.0 42.072254 1 GTATCAA 11715 0.0 34.93787 1 TATACCG 80 4.5205912E-4 29.65879 5 ATCAACG 14605 0.0 27.877844 3 TCAACGC 14870 0.0 27.412941 4 TATCAAC 14895 0.0 27.36693 2 CAACGCA 15090 0.0 26.95039 5 AACGCAG 15475 0.0 26.310558 6 TAAGGTG 1215 0.0 23.434107 5 TACATGG 10950 0.0 23.141983 2 ACGCAGA 17660 0.0 23.05526 7 GTAAGGT 1215 0.0 23.04354 4 GTACATG 11165 0.0 23.002527 1 CGCAGAG 17770 0.0 22.859133 8 GAGTACT 10120 0.0 22.110224 12-13 ACATGGG 11065 0.0 21.486208 3 GTACTTT 10365 0.0 21.381567 14-15 GCAGAGT 18995 0.0 21.309986 9 GGACCGT 185 6.611992E-6 20.52068 6 TATAGGG 440 0.0 20.49153 2 >>END_MODULE