FastQCFastQC Report
Fri 27 May 2016
SRR939091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544681
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75350.296107842201046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58020.2280050033776336No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44740.17581771546217384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36870.1448904597472139No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137700.025.5138841
GTACATG93750.025.0169561
CCGTATA950.001247859624.9276779
TACATGG95400.024.0387482
GGTATCA103950.023.1102581
GAGTACT73000.023.03271912-13
ACATGGG96150.023.0134873
ATCAACG158400.021.6869093
GTACTTT77500.021.66669814-15
TCAACGC159800.021.4672624
CAACGCA159350.021.4089455
TATCAAC161600.021.3454282
AACGCAG160450.021.2833276
AGTACTT74400.020.46669612-13
CATGGGG54450.020.44964
ACGCAGA174900.019.4711517
AGAGTAC129850.019.11282210-11
CGCAGAG178500.018.9723178
TACCGTA1300.007691285618.2170967
ACTTTTT97550.017.67679416-17
Produced by FastQC (version 0.11.5)