FastQCFastQC Report
Fri 27 May 2016
SRR939091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544681
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77770.3056178750892548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62180.2443528285077776No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46660.18336286552224032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39550.15542223170605668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96300.024.7147351
GGTATCA111150.024.4105551
GTATCAA146350.024.2312491
TACATGG97750.023.7361982
ACATGGG99200.023.0544343
GAGTACT83150.021.8846312-13
GTACTTT87200.020.7865814-15
CATGGGG55250.020.782714
AGTACTT84700.020.50377512-13
ATCAACG170650.020.4640053
TCAACGC172650.020.2269444
CAACGCA172850.020.203545
TATCAAC174900.020.1295072
AACGCAG176450.019.7913426
AGAGTAC143250.018.71500610-11
ATTCGCG4650.017.87860380-81
ACGCAGA195850.017.8309027
CGCAGAG197950.017.6417378
TACTTTT100500.017.25668714-15
ACTTTTT108500.016.81456816-17
Produced by FastQC (version 0.11.5)