##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939091_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2544681 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54183687464165 34.0 31.0 34.0 31.0 34.0 2 32.747127439549395 34.0 31.0 34.0 31.0 34.0 3 32.83619400624283 34.0 31.0 34.0 31.0 34.0 4 36.286406429725375 37.0 37.0 37.0 35.0 37.0 5 36.24663955914317 37.0 37.0 37.0 35.0 37.0 6 36.28081948189184 37.0 37.0 37.0 35.0 37.0 7 36.23041119888898 37.0 37.0 37.0 35.0 37.0 8 36.21679377493682 37.0 37.0 37.0 35.0 37.0 9 37.97250264374985 39.0 38.0 39.0 35.0 39.0 10-11 37.91463645148449 39.0 38.0 39.0 35.0 39.0 12-13 37.851880844789584 39.0 38.0 39.0 35.0 39.0 14-15 39.24689479742254 41.0 39.0 41.0 36.0 41.0 16-17 39.192303868343416 40.5 39.0 41.0 36.0 41.0 18-19 39.20877155132608 40.5 38.5 41.0 36.0 41.0 20-21 39.263733253794875 41.0 39.0 41.0 36.0 41.0 22-23 39.22431279205527 41.0 39.0 41.0 36.0 41.0 24-25 39.16920568825719 41.0 39.0 41.0 36.0 41.0 26-27 39.03811381465889 40.5 39.0 41.0 35.0 41.0 28-29 38.89170057071986 40.0 38.0 41.0 35.0 41.0 30-31 38.70919007136847 40.0 38.0 41.0 35.0 41.0 32-33 38.58149115743781 40.0 38.0 41.0 34.5 41.0 34-35 38.52996682098856 40.0 38.0 41.0 34.0 41.0 36-37 38.42575120417844 40.0 38.0 41.0 34.0 41.0 38-39 38.294159464388656 40.0 38.0 41.0 34.0 41.0 40-41 38.1269290728386 40.0 38.0 41.0 33.5 41.0 42-43 37.96286646538407 40.0 38.0 41.0 33.0 41.0 44-45 37.743162306002205 40.0 37.0 41.0 33.0 41.0 46-47 37.46183116862192 40.0 37.0 41.0 33.0 41.0 48-49 37.15193142087358 40.0 37.0 41.0 32.0 41.0 50-51 36.89758244746591 40.0 36.0 41.0 31.0 41.0 52-53 36.654958519358615 39.5 36.0 41.0 31.0 41.0 54-55 36.402081832654076 39.0 35.0 41.0 31.0 41.0 56-57 36.12209447863995 39.0 35.0 40.5 30.0 41.0 58-59 35.82061110999768 38.5 35.0 40.0 29.5 41.0 60-61 35.45760018642808 38.0 35.0 40.0 29.0 41.0 62-63 35.07346814787394 37.5 34.5 40.0 28.0 41.0 64-65 34.675498814979164 37.0 34.0 40.0 28.0 41.0 66-67 34.317111260704195 36.5 34.0 39.0 27.5 41.0 68-69 33.86271953144618 36.0 33.5 39.0 26.0 40.0 70-71 33.43870351529327 35.0 33.0 38.5 26.0 40.0 72-73 32.99649150522207 35.0 33.0 37.5 25.5 40.0 74-75 32.527412276823696 35.0 32.5 37.0 24.5 39.0 76-77 30.54757629738266 33.5 29.5 35.0 20.5 37.5 78-79 31.531520453840777 34.5 31.5 36.0 22.5 38.0 80-81 31.579966211874886 35.0 32.0 36.0 23.0 37.0 82-83 31.315502807621073 35.0 32.0 35.0 22.5 37.0 84-85 30.97479585849857 34.5 31.5 35.0 20.5 36.5 86-87 30.4594613234429 34.0 31.0 35.0 18.5 36.0 88-89 29.952551027024604 34.0 30.5 35.0 12.5 36.0 90-91 29.541476515130974 34.0 30.0 35.0 7.0 35.0 92-93 29.303493443775466 34.0 30.0 35.0 3.5 35.0 94-95 29.010925927454167 34.0 29.5 35.0 2.0 35.0 96-97 28.5025095483481 34.0 29.0 35.0 2.0 35.0 98-99 27.791431814046632 34.0 29.0 35.0 2.0 35.0 100-101 24.184561444047407 30.0 14.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14577748642034294 1101 2 0.1605957233667965 1101 3 0.1434296097090879 1101 4 0.07209139389570396 1101 5 0.125804715323099 1101 6 0.10918434597912352 1101 7 0.09078645878104652 1101 8 0.09502636796160857 1101 9 0.19253786864528877 1101 10-11 0.1809423364934304 1101 12-13 0.14806653556716753 1101 14-15 0.23163486860416782 1101 16-17 0.21114454353214285 1101 18-19 0.1333591373899523 1101 20-21 0.18772281836783122 1101 22-23 0.12101452544398228 1101 24-25 0.11791738428148335 1101 26-27 0.10543281332549981 1101 28-29 0.10439864626474815 1101 30-31 0.1187766373030783 1101 32-33 0.11769983826591357 1101 34-35 0.05235779051617584 1101 36-37 0.16552991661959027 1101 38-39 0.19910852295266324 1101 40-41 0.22648691115510644 1101 42-43 0.21963727104549946 1101 44-45 0.18628707243480136 1101 46-47 0.14013639138056533 1101 48-49 0.04377258051986388 1101 50-51 0.1291785116892541 1101 52-53 0.04937379765737404 1101 54-55 0.1160154867102392 1101 56-57 0.016827365072700218 1101 58-59 0.1545830856185617 1101 60-61 0.15899411642539008 1101 62-63 0.0690914722867575 1101 64-65 0.15025704673092832 1101 66-67 0.2415466474622292 1101 68-69 0.13079390987788742 1101 70-71 0.060857266398315346 1101 72-73 0.17588836014560627 1101 74-75 0.15650268937863387 1101 76-77 0.08492242042943232 1101 78-79 0.29544323024338937 1101 80-81 0.2588242645769583 1101 82-83 0.25908897191796143 1101 84-85 0.26537548893137597 1101 86-87 0.4039846765885713 1101 88-89 0.6378047196400054 1101 90-91 0.5400925110202586 1101 92-93 0.39443129848464764 1101 94-95 0.32103589982473224 1101 96-97 0.22277670104041292 1101 98-99 0.35689166731998867 1101 100-101 0.05721477786029894 1102 1 0.1630221411818411 1102 2 0.15428826791250572 1102 3 0.15910161544620394 1102 4 0.11965945224059027 1102 5 0.12643447397934438 1102 6 0.12607474864635293 1102 7 0.14440599819986488 1102 8 0.12589543901927414 1102 9 0.23651961284031842 1102 10-11 0.22322190245957785 1102 12-13 0.21252766940414602 1102 14-15 0.2233444855102391 1102 16-17 0.2810712957950017 1102 18-19 0.21155879658422094 1102 20-21 0.28917553979697175 1102 22-23 0.2701041818585139 1102 24-25 0.26154810433227027 1102 26-27 0.2510696940319832 1102 28-29 0.13441673100891904 1102 30-31 0.1693925383904613 1102 32-33 0.21394620528013064 1102 34-35 0.23694060778871062 1102 36-37 0.3857535169228967 1102 38-39 0.29624216501782286 1102 40-41 0.21249559824708797 1102 42-43 0.13238321205255232 1102 44-45 0.18203193526542094 1102 46-47 0.21208482724346567 1102 48-49 0.22303729873832623 1102 50-51 0.23974063142851776 1102 52-53 0.31588640681143687 1102 54-55 0.335635542601068 1102 56-57 0.32328215485708256 1102 58-59 0.292671703332239 1102 60-61 0.33256654011007214 1102 62-63 0.2675613622979611 1102 64-65 0.3024757586431761 1102 66-67 0.42090810138025603 1102 68-69 0.312869309033438 1102 70-71 0.40390179483799216 1102 72-73 0.20858749382139052 1102 74-75 0.2675176473289298 1102 76-77 0.20169104508422464 1102 78-79 0.16178070768885888 1102 80-81 0.11149620534386884 1102 82-83 0.15155619074912252 1102 84-85 0.19483881859677155 1102 86-87 0.3768227108040172 1102 88-89 0.3543767271864162 1102 90-91 0.472840228427831 1102 92-93 0.4192120857261443 1102 94-95 0.2953776036231055 1102 96-97 0.2251834770215062 1102 98-99 -0.028181901156138167 1102 100-101 -0.1673991428694741 1103 1 0.13842632789854292 1103 2 0.12932206681173852 1103 3 0.18886648185689836 1103 4 0.1131430932094517 1103 5 0.14233709109680603 1103 6 0.09704600780521844 1103 7 0.1571738647114529 1103 8 0.19621251484789326 1103 9 0.14349283068759888 1103 10-11 0.21756206525634525 1103 12-13 0.21681341228342887 1103 14-15 0.2674754256907832 1103 16-17 0.23516362628040355 1103 18-19 0.21996975399831342 1103 20-21 0.2036186833463134 1103 22-23 0.21793125684405368 1103 24-25 0.1900777111975458 1103 26-27 0.21791968110278503 1103 28-29 0.3184648029806141 1103 30-31 0.24005240490932778 1103 32-33 0.2918368223983947 1103 34-35 0.33120703672246776 1103 36-37 0.44793156307459014 1103 38-39 0.3441114545100774 1103 40-41 0.4746794266540135 1103 42-43 0.433979781679092 1103 44-45 0.50906102668106 1103 46-47 0.5150144493124316 1103 48-49 0.5625283632333193 1103 50-51 0.447134053446014 1103 52-53 0.5106773429039677 1103 54-55 0.5282483274099903 1103 56-57 0.5247807091921857 1103 58-59 0.49484845959622703 1103 60-61 0.5528057677786862 1103 62-63 0.5237287137036049 1103 64-65 0.44305067251082164 1103 66-67 0.4309721570419498 1103 68-69 0.4728756331223778 1103 70-71 0.4574419401666958 1103 72-73 0.49904968403858163 1103 74-75 0.39681473737866213 1103 76-77 0.4974499630503999 1103 78-79 0.5769196425448762 1103 80-81 0.46246514755710066 1103 82-83 0.5201687952163816 1103 84-85 0.47397991917020477 1103 86-87 0.5864070212407704 1103 88-89 0.605269792463023 1103 90-91 0.7706662015500463 1103 92-93 0.7277654564473508 1103 94-95 0.48349448281460994 1103 96-97 0.5231475368682474 1103 98-99 0.5362140240117732 1103 100-101 0.44634954373977465 1104 1 0.08219376813688939 1104 2 0.13354161994193703 1104 3 0.11851185992188107 1104 4 0.09663734402911928 1104 5 0.07532484271528261 1104 6 0.11567230971251519 1104 7 0.12747985676686824 1104 8 0.09642270878353543 1104 9 0.16061928679197734 1104 10-11 0.1829698998786995 1104 12-13 0.12043142046469058 1104 14-15 0.13210194939109243 1104 16-17 0.17360294022682865 1104 18-19 0.19797217883066764 1104 20-21 0.14833178077182652 1104 22-23 0.20527974890632805 1104 24-25 0.18312757661308154 1104 26-27 0.17549373586683714 1104 28-29 0.09081181968836916 1104 30-31 0.20580644822322824 1104 32-33 0.18822461375152244 1104 34-35 0.16271175335055688 1104 36-37 0.24353432083156434 1104 38-39 0.22327609819726746 1104 40-41 0.3102595897384859 1104 42-43 0.3360338748768257 1104 44-45 0.2549181802303977 1104 46-47 0.24930740965733378 1104 48-49 0.08892173076039711 1104 50-51 0.24528341418449884 1104 52-53 0.2928027468630958 1104 54-55 0.28201626139247793 1104 56-57 0.347723079454056 1104 58-59 0.32933729137798906 1104 60-61 0.38610990750984797 1104 62-63 0.45926532150107846 1104 64-65 0.3727253421430916 1104 66-67 0.40157890197881585 1104 68-69 0.28708186691650184 1104 70-71 0.4058383297214121 1104 72-73 0.20896657435958588 1104 74-75 0.12079677214529738 1104 76-77 0.1391352332910678 1104 78-79 0.3523686264311898 1104 80-81 0.34179476448609947 1104 82-83 0.24436223868600848 1104 84-85 0.22468076155257677 1104 86-87 0.28416536404732895 1104 88-89 0.3848685783226635 1104 90-91 0.5476534335615959 1104 92-93 0.4974257800692108 1104 94-95 0.4238343198555299 1104 96-97 0.3404829721409399 1104 98-99 0.30213012120726646 1104 100-101 0.014270124602330014 1105 1 0.08373226812512513 1105 2 0.10681082583190005 1105 3 0.12044766485214353 1105 4 0.08486704729635619 1105 5 0.09370083946922136 1105 6 0.10268437072915049 1105 7 0.09862912287371017 1105 8 0.11318669862193786 1105 9 0.17418572529314247 1105 10-11 0.1394620684506691 1105 12-13 0.16895168926482285 1105 14-15 0.12100148453328785 1105 16-17 0.1989690782316842 1105 18-19 0.1613065559623692 1105 20-21 0.19912561883312918 1105 22-23 0.2219992670537252 1105 24-25 0.135298089162184 1105 26-27 0.1946783088209969 1105 28-29 0.18324990480350323 1105 30-31 0.18977440361334175 1105 32-33 0.24799015605623254 1105 34-35 0.3239993668452499 1105 36-37 0.18081692409409555 1105 38-39 0.1914213199529584 1105 40-41 0.17330993610313072 1105 42-43 0.2274505444837729 1105 44-45 0.1463297043524321 1105 46-47 0.2094635310202051 1105 48-49 0.26485449691427476 1105 50-51 0.1758307614665071 1105 52-53 0.2943193785404574 1105 54-55 0.347402694659948 1105 56-57 0.3137108728937079 1105 58-59 0.429786345196824 1105 60-61 0.36104790441667234 1105 62-63 0.42823676111954256 1105 64-65 0.37760381314019753 1105 66-67 0.4382103441259275 1105 68-69 0.29332113490511347 1105 70-71 0.273539643143188 1105 72-73 0.32810255923480014 1105 74-75 0.24156001662345972 1105 76-77 0.3069726815421916 1105 78-79 0.30924523623289346 1105 80-81 0.1730259834661787 1105 82-83 0.14820654072303086 1105 84-85 0.2910799365108261 1105 86-87 0.4336475812514742 1105 88-89 0.43292942882721164 1105 90-91 0.2931189749842211 1105 92-93 0.1382871048404546 1105 94-95 0.08582171929805327 1105 96-97 0.19296761654382877 1105 98-99 0.10693265954848385 1105 100-101 -0.26957062705010415 1106 1 -0.044686594952828784 1106 2 0.025971256924151476 1106 3 0.07461130679987349 1106 4 -0.027568037581907845 1106 5 -0.012250411368391667 1106 6 0.06686378774947599 1106 7 0.08745115771846912 1106 8 0.004433627368868542 1106 9 0.05444036009323128 1106 10-11 0.06251984988912795 1106 12-13 0.039931488795758696 1106 14-15 -0.002265700523672365 1106 16-17 0.028279302712356014 1106 18-19 -0.010850440910530779 1106 20-21 0.06711653071608481 1106 22-23 0.06246512030366347 1106 24-25 0.17873119168619667 1106 26-27 0.1831876342621399 1106 28-29 0.1436275991300704 1106 30-31 0.13144048860329605 1106 32-33 0.16573362266333191 1106 34-35 0.08114945339875135 1106 36-37 0.21861811629869266 1106 38-39 0.3333072787423319 1106 40-41 0.19149803639350438 1106 42-43 0.19799792554250928 1106 44-45 0.3231131251699395 1106 46-47 0.3953361158984734 1106 48-49 0.39381117828573053 1106 50-51 0.39426456200258286 1106 52-53 0.4346040976604044 1106 54-55 0.3878360903490261 1106 56-57 0.32042679594485435 1106 58-59 0.34586226098864614 1106 60-61 0.27566532400568633 1106 62-63 0.20217543377980718 1106 64-65 0.35579128521970915 1106 66-67 0.4080645992074565 1106 68-69 0.3210517580902845 1106 70-71 0.3296838943158562 1106 72-73 0.27553038478762915 1106 74-75 0.40749109491249413 1106 76-77 0.2934302005281175 1106 78-79 0.4258268617633867 1106 80-81 0.4948371153625395 1106 82-83 0.5509088944197025 1106 84-85 0.6853918362204503 1106 86-87 0.7084381776329813 1106 88-89 0.9443171466069735 1106 90-91 0.9310360371910527 1106 92-93 0.9502845361560688 1106 94-95 0.7979695722129527 1106 96-97 0.8850190041918076 1106 98-99 0.9374856945049252 1106 100-101 0.5051410939684331 1107 1 -0.024844444839885682 1107 2 -0.00353801523502284 1107 3 0.044168055660300354 1107 4 0.047892188845438 1107 5 0.06223796292655237 1107 6 0.07640241160144257 1107 7 0.12834198835785315 1107 8 0.05607772794913757 1107 9 0.07809823026947527 1107 10-11 0.04252755978078682 1107 12-13 0.059189986584236465 1107 14-15 0.1793445171116872 1107 16-17 0.1393311618783315 1107 18-19 0.15122602227810944 1107 20-21 0.15525749999825678 1107 22-23 0.10419490889032801 1107 24-25 0.08275352331974517 1107 26-27 0.07829151490760466 1107 28-29 0.06555716859348593 1107 30-31 0.16649310523231264 1107 32-33 0.10545410527549848 1107 34-35 0.02479758476447813 1107 36-37 0.020631818264043034 1107 38-39 0.09285706164801155 1107 40-41 0.09852046270993498 1107 42-43 0.12170550423074644 1107 44-45 0.11107859297099765 1107 46-47 0.13996590583774093 1107 48-49 0.15152918561688722 1107 50-51 0.1517556685749213 1107 52-53 0.13351084854974715 1107 54-55 0.062375863921680264 1107 56-57 0.10068676808104016 1107 58-59 0.031044390271354416 1107 60-61 0.04909426450426224 1107 62-63 0.025120815284189746 1107 64-65 0.11064107759368369 1107 66-67 0.11137850000684324 1107 68-69 0.031283934038121686 1107 70-71 0.16484370549113692 1107 72-73 0.09239915431597723 1107 74-75 0.026339412938767737 1107 76-77 0.09182464940612789 1107 78-79 0.41992203158389074 1107 80-81 0.3452019826036157 1107 82-83 0.3058848179159348 1107 84-85 0.433688558840192 1107 86-87 0.4120364652254658 1107 88-89 0.436709715353512 1107 90-91 0.4523606506820137 1107 92-93 0.5515800008461191 1107 94-95 0.563193564854739 1107 96-97 0.43127564890106385 1107 98-99 0.45292373808610265 1107 100-101 0.21373999023657575 1108 1 -0.03137362333946214 1108 2 -0.06677038878191865 1108 3 -0.04186372014946471 1108 4 -0.07663379395991399 1108 5 -0.023820659302280944 1108 6 -0.07930940664506636 1108 7 -0.009368820722059468 1108 8 -0.048333171050103374 1108 9 -0.0785276600289393 1108 10-11 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2316 76-77 -0.19819891983643245 2316 78-79 -0.14539267890593166 2316 80-81 -0.06044876680023137 2316 82-83 -0.1741947641638184 2316 84-85 -0.3298196760402554 2316 86-87 -0.3608277579498811 2316 88-89 -0.6751222374968258 2316 90-91 -0.7392397356940172 2316 92-93 -0.7020884500065385 2316 94-95 -0.49530557718113144 2316 96-97 -0.6950539160295683 2316 98-99 -0.627126830223034 2316 100-101 -0.35517819499526126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 16.0 5 42.0 6 108.0 7 245.0 8 561.0 9 1056.0 10 1722.0 11 2858.0 12 4198.0 13 6167.0 14 7403.0 15 9970.0 16 13109.0 17 18726.0 18 18477.0 19 12635.0 20 11367.0 21 12201.0 22 12372.0 23 13555.0 24 15377.0 25 18305.0 26 21673.0 27 26115.0 28 32140.0 29 39594.0 30 48492.0 31 60487.0 32 76488.0 33 99742.0 34 141217.0 35 213664.0 36 358415.0 37 616891.0 38 570432.0 39 58857.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79921335761982 18.93080047211162 14.555762762728419 23.714223407540143 2 15.811176332121788 20.353985430786807 39.25317161561704 24.581666621474362 3 19.31770622722455 23.88421181279697 29.812892067807322 26.985189892171157 4 13.606656394259243 16.617524947134825 35.83447198293224 33.94134667567369 5 14.525985771890465 36.63429718695585 33.53874218418733 15.300974856966354 6 32.82379991833947 35.07091851591614 17.048856025568625 15.056425540175763 7 29.0628962923054 32.51036180959421 20.303330751477297 18.123411146623095 8 26.32923340882413 34.624300649079395 19.692802359116918 19.353663582979554 9 26.512360488406994 17.043708032558893 20.322861686789032 36.12106979224508 10-11 25.614350089461112 25.958676156264776 26.750386394208153 21.67658736006596 12-13 26.302628895331086 23.338681744391536 27.417837442099813 22.940851918177565 14-15 23.073623550859264 24.91469863590519 25.764440712874777 26.247237100360778 16-17 22.831784783617636 28.06598499826498 26.393468778871977 22.70876143924541 18-19 22.67412798465819 26.582870930726067 28.638316016159205 22.104685068456543 20-21 23.39674403196314 25.77674765520708 28.482273416589347 22.344234896240433 22-23 23.017836027384178 25.61486095899643 28.459736210550556 22.907566803068832 24-25 23.044151706144945 25.85538121944716 28.48391510174504 22.616551972662855 26-27 22.777157195334137 26.058587567457643 28.537393612683854 22.62686162452437 28-29 22.508852779582195 26.47675681156106 28.571262959875916 22.443127448980835 30-31 22.732676310064264 25.93829776531395 28.365763999798787 22.963261924822998 32-33 22.606409211999463 26.032496804118082 28.33924959552887 23.02184438835359 34-35 23.339567273992795 26.10923302485906 27.970670543932773 22.580529157215373 36-37 23.32207335358911 25.720179774450557 28.115514931314912 22.842231940645423 38-39 22.834786180412458 26.031246284357106 28.288391864842477 22.84557567038796 40-41 22.92337520285277 26.027076644448666 28.2603731508415 22.789175001857068 42-43 23.0610844874772 26.046776503157794 27.72417351941121 23.167965489953797 44-45 22.94349012824566 26.130895970834022 27.78472601973938 23.140887881180934 46-47 23.179652856264827 26.481518887454946 27.319884998027234 23.018943258252992 48-49 23.246029679770892 26.269023888236653 27.382668625014123 23.102277806978332 50-51 23.00279406447962 26.5771138217772 27.32253171322131 23.097560400521875 52-53 23.40778542205127 26.557711208368524 26.964108243122215 23.070395126457992 54-55 22.888016218928815 26.335756819813565 27.172973744056723 23.603253217200898 56-57 23.126985268487484 26.66222210170941 27.059757195499163 23.151035434303946 58-59 23.281306891946247 26.262869799492762 27.127946689648923 23.327876618912065 60-61 23.09246086628096 26.34880603297337 27.555781933543127 23.002951167202546 62-63 22.805633100962382 26.446587267418636 27.393113915474405 23.354665716144577 64-65 22.639992737653223 26.446928278823815 27.30502878215789 23.608050201365067 66-67 22.719281612442096 26.449002048465616 27.07780730668527 23.75390903240702 68-69 22.699405308698132 26.337780336575822 27.330373467709528 23.63244088701651 70-71 22.785536884794944 26.412535171416447 27.140799629029978 23.661128314758635 72-73 22.86404464842548 26.219416107559258 27.222901416719818 23.693637827295444 74-75 22.709192232739586 26.681261816314105 26.834247593313272 23.77529835763304 76-77 22.735178155154546 26.399045018583305 26.985451971894054 23.88032485436809 78-79 22.754384654984733 26.49897629162131 26.999139377601022 23.747499675792934 80-81 22.729350208794934 26.690932413006042 26.95063903903243 23.629078339166597 82-83 22.87296250274103 26.488545058565645 26.852443503391015 23.786048935302315 84-85 22.677262886782273 26.397611331243482 26.810138481011965 24.11498730096228 86-87 22.692689075580663 26.207142366782126 26.988282795220275 24.111885762416936 88-89 22.567280554560853 26.28663425086046 26.781828602657782 24.364256591920906 90-91 22.470707663706857 26.290882821975032 26.88702694129539 24.351382573022722 92-93 22.563004950325798 26.00940943088741 27.060071576751664 24.367514042035133 94-95 22.338910063776165 25.911911944954984 27.0939854543654 24.65519253690345 96-97 22.3004420397767 25.841240187127617 27.18374664324264 24.67457112985304 98-99 22.205398204266412 25.97889487423086 26.818187623605283 24.997519297897444 100-101 22.25170224016043 25.27112152245004 27.383367100103488 25.093809137286037 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 365.0 1 254.5 2 217.0 3 296.0 4 270.5 5 293.5 6 520.5 7 1057.0 8 1736.5 9 2126.0 10 2063.0 11 1973.5 12 2111.5 13 2310.0 14 2625.0 15 2846.0 16 2920.5 17 3093.0 18 3324.0 19 3625.5 20 3954.5 21 4386.5 22 5200.0 23 6383.0 24 7848.5 25 9683.0 26 12386.5 27 15894.0 28 19240.0 29 22349.5 30 25954.0 31 30473.0 32 35662.5 33 38811.5 34 40211.5 35 42168.5 36 46040.5 37 51070.5 38 55419.0 39 58343.5 40 61045.5 41 67744.0 42 75039.0 43 80020.5 44 84174.5 45 89139.5 46 91339.5 47 95313.0 48 107420.5 49 109111.5 50 102473.5 51 99853.5 52 97178.5 53 97476.5 54 97444.5 55 91473.0 56 82104.0 57 73353.5 58 66493.5 59 58760.0 60 50191.5 61 42339.5 62 36003.0 63 28879.0 64 21913.0 65 17056.5 66 12780.5 67 10505.5 68 8085.5 69 5385.5 70 4093.5 71 3333.0 72 2874.5 73 2397.0 74 1960.5 75 1704.5 76 1212.5 77 666.0 78 375.0 79 237.5 80 151.0 81 92.0 82 62.5 83 33.0 84 17.5 85 15.5 86 8.5 87 4.5 88 5.5 89 5.5 90 3.0 91 2.5 92 1.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3473912840155603 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010413878989154238 16-17 0.0016505015756395401 18-19 3.9297656562846185E-5 20-21 0.0 22-23 0.0 24-25 4.519230504727312E-4 26-27 0.0011199832120411162 28-29 0.0 30-31 0.0028687289290877716 32-33 0.0 34-35 0.010865802039626971 36-37 0.028117473270716446 38-39 0.021161788059092673 40-41 0.013675584483870473 42-43 0.010315634847747125 44-45 0.002849080100806349 46-47 0.0011396320403225394 48-49 7.270066464126545E-4 50-51 3.9297656562846185E-5 52-53 7.859531312569237E-5 54-55 0.0 56-57 0.0 58-59 0.004303093393631658 60-61 0.010374581332591393 62-63 0.004499581676445889 64-65 0.001925585171579463 66-67 0.0036743308886261184 68-69 0.0032813543229976566 70-71 3.9297656562846185E-5 72-73 0.0 74-75 0.0 76-77 0.21904513768130465 78-79 4.322742221913081E-4 80-81 0.0031045148684648487 82-83 5.894648484426928E-4 84-85 0.0 86-87 0.11083904033550766 88-89 0.4096191231828273 90-91 0.39529512736566974 92-93 0.0 94-95 0.0 96-97 1.9648828281423092E-5 98-99 0.0015129597776695782 100-101 7.663043029755007E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2544681.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.29881890768221 #Duplication Level Percentage of deduplicated Percentage of total 1 67.36329766506762 29.841145242891226 2 15.820722717814798 14.016786613302635 3 6.665295066402995 8.857940973385618 4 3.315898360869169 5.875615240176944 5 1.8977595572871049 4.203425347929231 6 1.1785770789022894 3.132574355222256 7 0.7880857193419596 2.443788659540196 8 0.5541092963541996 1.9637109899406324 9 0.3912014781797328 1.559678709447142 >10 1.8155569703566594 13.930359237651396 >50 0.12065847442618545 3.6844923054913963 >100 0.08127274389245692 6.846719367271841 >500 0.005339909015140458 1.5733839574769044 >1k 0.00204696512246864 1.5186222476197668 >5k 1.7799696717118606E-4 0.5517567526529411 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7777 0.3056178750892548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6218 0.2443528285077776 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4666 0.18336286552224032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3955 0.15542223170605668 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.9648828281423092E-5 0.0 16-17 0.0 0.0 0.0 3.9297656562846185E-5 0.0 18-19 0.0 0.0 0.0 1.3754179796996165E-4 0.0 20-21 0.0 0.0 0.0 1.9648828281423094E-4 0.0 22-23 0.0 0.0 0.0 4.5192305047273115E-4 0.0 24-25 0.0 0.0 0.0 5.501671918798466E-4 0.0 26-27 0.0 0.0 0.0 0.0011592808686039626 0.0 28-29 0.0 0.0 0.0 0.004047658625973158 0.0 30-31 0.0 0.0 0.0 0.01294857783745782 0.0 32-33 0.0 0.0 0.0 0.029767974846355988 0.0 34-35 0.0 0.0 0.0 0.053385866440626546 0.0 36-37 0.0 0.0 0.0 0.09185827221565296 0.0 38-39 0.0 0.0 0.0 0.14901671368631275 0.0 40-41 0.0 0.0 0.0 0.21122490402529825 0.0 42-43 0.0 0.0 0.0 0.27755934830338264 0.0 44-45 0.0 0.0 0.0 0.3624422864791304 0.0 46-47 0.0 0.0 0.0 0.4643804076031534 0.0 48-49 0.0 0.0 0.0 0.5713093311106578 0.0 50-51 0.0 0.0 0.0 0.6917173508192186 0.0 52-53 0.0 0.0 0.0 0.8110250361440197 0.0 54-55 0.0 0.0 0.0 0.9520643255480745 0.0 56-57 0.0 0.0 0.0 1.1195312889906437 0.0 58-59 0.0 0.0 0.0 1.3027762615431953 0.0 60-61 0.0 0.0 0.0 1.5017206478926042 0.0 62-63 0.0 0.0 0.0 1.7105876925241317 0.0 64-65 0.0 0.0 0.0 1.9301240509124717 0.0 66-67 0.0 0.0 0.0 2.189960156106011 0.0 68-69 0.0 0.0 0.0 2.479682129115594 0.0 70-71 0.0 0.0 0.0 2.795694234365722 0.0 72-73 0.0 0.0 0.0 3.118406590059815 0.0 74-75 0.0 0.0 0.0 3.463125633429102 3.9297656562846185E-5 76-77 0.0 0.0 0.0 3.841542417301029 3.9297656562846185E-5 78-79 0.0 0.0 0.0 4.273364716441865 3.9297656562846185E-5 80-81 0.0 0.0 0.0 4.7328525658029434 3.9297656562846185E-5 82-83 0.0 0.0 0.0 5.216272688010796 3.9297656562846185E-5 84-85 0.0 0.0 0.0 5.720304431085861 3.9297656562846185E-5 86-87 0.0 0.0 0.0 6.254300637290097 3.9297656562846185E-5 88-89 0.0 0.0 0.0 6.831013396178146 3.9297656562846185E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9630 0.0 24.714735 1 GGTATCA 11115 0.0 24.410555 1 GTATCAA 14635 0.0 24.231249 1 TACATGG 9775 0.0 23.736198 2 ACATGGG 9920 0.0 23.054434 3 GAGTACT 8315 0.0 21.88463 12-13 GTACTTT 8720 0.0 20.78658 14-15 CATGGGG 5525 0.0 20.78271 4 AGTACTT 8470 0.0 20.503775 12-13 ATCAACG 17065 0.0 20.464005 3 TCAACGC 17265 0.0 20.226944 4 CAACGCA 17285 0.0 20.20354 5 TATCAAC 17490 0.0 20.129507 2 AACGCAG 17645 0.0 19.791342 6 AGAGTAC 14325 0.0 18.715006 10-11 ATTCGCG 465 0.0 17.878603 80-81 ACGCAGA 19585 0.0 17.830902 7 CGCAGAG 19795 0.0 17.641737 8 TACTTTT 10050 0.0 17.256687 14-15 ACTTTTT 10850 0.0 16.814568 16-17 >>END_MODULE