FastQCFastQC Report
Fri 27 May 2016
SRR939090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504865
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74050.2956247143059606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.22619981515969922No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42720.1705481133713793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36560.1459559696829969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128100.026.4673121
GGTATCA96600.025.5615371
GTACATG88750.025.2542441
TACATGG91250.023.6690062
ACATGGG92350.022.5156423
ATCAACG151500.022.1348883
TCAACGC153150.021.9273414
CAACGCA153850.021.8878355
TATCAAC154900.021.8320672
AACGCAG154900.021.7587346
GAGTACT76650.021.3686812-13
AGTACTT77300.020.63783512-13
ATACCGT2351.17492164E-720.1436066
CATGGGG52300.020.1051834
GTACTTT81350.019.7277514-15
ACGCAGA170650.019.695057
CGCAGAG174650.019.2439758
AGAGTAC131050.019.16236110-11
GTATAGA7350.018.0897031
GTCTACG2905.2385076E-818.011731
Produced by FastQC (version 0.11.5)