##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939090_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2504865 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.273530110405154 34.0 31.0 34.0 31.0 34.0 2 32.375351965075964 34.0 31.0 34.0 31.0 34.0 3 32.44330093637781 34.0 31.0 34.0 31.0 34.0 4 35.86850548831973 37.0 35.0 37.0 35.0 37.0 5 35.90191806744076 37.0 35.0 37.0 35.0 37.0 6 35.98628189543149 37.0 37.0 37.0 35.0 37.0 7 35.922411786663154 37.0 37.0 37.0 35.0 37.0 8 35.92042684935116 37.0 37.0 37.0 35.0 37.0 9 37.628451034287274 39.0 38.0 39.0 35.0 39.0 10-11 37.54710174001393 39.0 38.0 39.0 35.0 39.0 12-13 37.462097757763395 39.0 38.0 39.0 35.0 39.0 14-15 38.80866553686526 40.5 38.0 41.0 35.0 41.0 16-17 38.73074936174205 40.0 38.0 41.0 35.0 41.0 18-19 38.627846410884416 40.0 38.0 41.0 34.0 41.0 20-21 38.60811201402072 40.0 38.5 41.0 34.0 41.0 22-23 38.649888317334465 40.0 38.5 41.0 34.0 41.0 24-25 38.59659223151746 40.5 38.0 41.0 34.0 41.0 26-27 38.49673974445729 40.0 38.0 41.0 34.0 41.0 28-29 38.36224966215744 40.0 38.0 41.0 34.0 41.0 30-31 38.17420220251391 40.0 38.0 41.0 34.0 41.0 32-33 37.993306226084044 40.0 38.0 41.0 33.5 41.0 34-35 37.6963856335571 40.0 38.0 41.0 33.0 41.0 36-37 37.44609649621836 40.0 37.5 41.0 32.5 41.0 38-39 37.26420425851293 40.0 37.0 41.0 31.5 41.0 40-41 37.075150557016045 40.0 37.0 41.0 31.0 41.0 42-43 36.86710960470924 40.0 36.5 41.0 31.0 41.0 44-45 36.60508630205619 40.0 36.0 41.0 30.0 41.0 46-47 36.48552436957681 40.0 36.0 41.0 30.0 41.0 48-49 36.37591107704407 40.0 36.0 41.0 30.0 41.0 50-51 35.23156457533639 38.5 34.5 40.0 27.5 40.5 52-53 35.35687791557629 38.5 35.0 40.0 28.0 40.5 54-55 35.86630437169269 39.0 35.0 41.0 29.0 41.0 56-57 35.76147556854362 39.0 35.0 41.0 28.0 41.0 58-59 35.555805801909486 39.0 35.0 41.0 28.0 41.0 60-61 35.25183313272372 38.5 35.0 41.0 28.0 41.0 62-63 34.880708740790425 38.0 35.0 40.0 26.5 41.0 64-65 34.51371730612229 37.0 34.0 40.0 26.0 41.0 66-67 34.13136436494581 37.0 34.0 39.5 26.0 41.0 68-69 33.724370774472874 36.0 34.0 39.0 25.5 41.0 70-71 33.2992249083284 35.5 34.0 39.0 24.5 40.5 72-73 32.864669553049765 35.0 33.0 38.0 23.5 40.0 74-75 32.437331552798256 35.0 33.0 37.0 22.5 39.5 76-77 32.019563928594955 35.0 32.5 37.0 21.0 39.0 78-79 31.603071622622377 35.0 32.0 36.0 20.0 39.0 80-81 31.181427342391707 35.0 32.0 36.0 18.0 37.5 82-83 30.819190255762287 35.0 31.5 35.0 16.5 37.0 84-85 30.465272379948622 35.0 31.0 35.0 10.0 37.0 86-87 30.08648909222653 34.0 31.0 35.0 6.0 36.0 88-89 29.690531425845304 34.0 30.5 35.0 2.0 36.0 90-91 29.312788114329514 34.0 30.0 35.0 2.0 35.5 92-93 28.93282612036976 34.0 29.5 35.0 2.0 35.0 94-95 28.47766646106676 34.0 29.0 35.0 2.0 35.0 96-97 27.912253953805894 34.0 29.0 35.0 2.0 35.0 98-99 27.241818221740495 34.0 27.0 35.0 2.0 35.0 100-101 24.352369289362898 31.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16639586366098058 1101 2 0.22671486040249533 1101 3 0.19850891129274828 1101 4 0.2689736596361527 1101 5 0.21935119704375694 1101 6 0.206270671813769 1101 7 0.24941248376003244 1101 8 0.35219764559386846 1101 9 0.4152325291896517 1101 10-11 0.3349181708111004 1101 12-13 0.3185889635532533 1101 14-15 0.44687108320498226 1101 16-17 0.4101587846244925 1101 18-19 0.6010703402131554 1101 20-21 0.5483448038016903 1101 22-23 0.5064625007341661 1101 24-25 0.5966259191055272 1101 26-27 0.5287548155824666 1101 28-29 0.6059529454255639 1101 30-31 0.5120836334849912 1101 32-33 0.512803055474734 1101 34-35 0.48015980142131554 1101 36-37 0.4859885158959969 1101 38-39 0.4028504180221404 1101 40-41 0.48507335351038705 1101 42-43 0.37662508354163293 1101 44-45 0.3616901143449738 1101 46-47 0.4434613489999535 1101 48-49 0.3481328907904455 1101 50-51 0.43041896615224573 1101 52-53 0.436131925078854 1101 54-55 0.4805745378927071 1101 56-57 0.40936924384066486 1101 58-59 0.45278632611368863 1101 60-61 0.34133878670449036 1101 62-63 0.23180930442385517 1101 64-65 0.16592223600672895 1101 66-67 0.34903287615708223 1101 68-69 0.19910779663663902 1101 70-71 0.05451506557478325 1101 72-73 -0.02855999662907749 1101 74-75 0.06520274523659708 1101 76-77 0.015401762677974773 1101 78-79 -0.12593452453010912 1101 80-81 -0.30510841594559324 1101 82-83 -0.19200661936499586 1101 84-85 -0.3684916155388258 1101 86-87 -0.3667984308863055 1101 88-89 -0.4194458072343501 1101 90-91 -0.35169892393784963 1101 92-93 -0.3359235117911439 1101 94-95 -0.38529805634877334 1101 96-97 -0.6301224234762977 1101 98-99 -0.7794511024726027 1101 100-101 -0.9031793591700961 1102 1 0.17266268665785844 1102 2 0.13533804613814482 1102 3 0.2026045882237213 1102 4 0.03446369136538863 1102 5 0.14902613112802499 1102 6 0.15980113156576436 1102 7 0.1542667410575973 1102 8 0.26867167419413107 1102 9 0.29369141281968325 1102 10-11 0.3107676392010674 1102 12-13 0.339897759968963 1102 14-15 0.3626675304366529 1102 16-17 0.35059799497637556 1102 18-19 0.3933639592501592 1102 20-21 0.5051328705560465 1102 22-23 0.39693094530922934 1102 24-25 0.4110065819876567 1102 26-27 0.4650278716620093 1102 28-29 0.39881393480756344 1102 30-31 0.408070842346973 1102 32-33 0.537046594977781 1102 34-35 0.5047485134151586 1102 36-37 0.4347392800479568 1102 38-39 0.3434310270085277 1102 40-41 0.2551753780635835 1102 42-43 0.22069735089647935 1102 44-45 0.32094511002310355 1102 46-47 0.30420822235872436 1102 48-49 0.24642942570258697 1102 50-51 0.067549115493172 1102 52-53 0.044871914521685596 1102 54-55 0.09277180164045262 1102 56-57 0.07834627244491799 1102 58-59 0.034878999267078825 1102 60-61 0.029654242840500444 1102 62-63 -0.07963617141917467 1102 64-65 -0.08982782714540605 1102 66-67 -0.06595691073275134 1102 68-69 -0.23308012636614706 1102 70-71 -0.16859666331986745 1102 72-73 -0.07190722417255557 1102 74-75 -0.23787113233127855 1102 76-77 -0.39043740159171847 1102 78-79 -0.3075525244652937 1102 80-81 -0.2140566853550503 1102 82-83 -0.46093883103941735 1102 84-85 -0.44616719068942245 1102 86-87 -0.5542805895750824 1102 88-89 -0.4880705379221162 1102 90-91 -0.4520302451338374 1102 92-93 -0.5120504944241979 1102 94-95 -0.5505801854703662 1102 96-97 -0.4790478604469115 1102 98-99 -0.5362210337977089 1102 100-101 -0.45924103206022693 1103 1 0.10484475160379247 1103 2 0.16274281804887636 1103 3 0.22674165363469 1103 4 0.17220339781003702 1103 5 0.2712439087603613 1103 6 0.21702087266385917 1103 7 0.22155225825859048 1103 8 0.17704391802212882 1103 9 0.18243868721918943 1103 10-11 0.16788535327911802 1103 12-13 0.23656208569070714 1103 14-15 0.29539191668137477 1103 16-17 0.35009065867603084 1103 18-19 0.3035643576270246 1103 20-21 0.3717846964069693 1103 22-23 0.282135477298219 1103 24-25 0.26370143874077456 1103 26-27 0.2749401658856314 1103 28-29 0.345376771740014 1103 30-31 0.25863635062626145 1103 32-33 0.23611145135198797 1103 34-35 0.11901995997272508 1103 36-37 0.1152840470672416 1103 38-39 0.1583274455780881 1103 40-41 0.08362337033372569 1103 42-43 0.08524846214920245 1103 44-45 0.05625162597367961 1103 46-47 0.2966682554965132 1103 48-49 0.20886910080067622 1103 50-51 0.18731417562000985 1103 52-53 0.17595058491090043 1103 54-55 0.20690169351204446 1103 56-57 0.22929147022103535 1103 58-59 0.24371511188045503 1103 60-61 0.31952766819263445 1103 62-63 0.07310854703016645 1103 64-65 0.11607665024545355 1103 66-67 0.04484111753426134 1103 68-69 0.057632938948366075 1103 70-71 0.07061697725567484 1103 72-73 0.07775407798416012 1103 74-75 0.1016074684754642 1103 76-77 0.18649342585408846 1103 78-79 0.2504669198738938 1103 80-81 0.1726712549349223 1103 82-83 0.07684410609867598 1103 84-85 -0.04528776471035911 1103 86-87 0.03955778791326381 1103 88-89 0.015476187395915275 1103 90-91 0.102435369241487 1103 92-93 -0.05053097860796285 1103 94-95 -0.21250302987775882 1103 96-97 -0.329508629023195 1103 98-99 -0.3855641124086766 1103 100-101 -0.3375869973103285 1104 1 0.18300167778923537 1104 2 0.24069691905621937 1104 3 0.30893376542039874 1104 4 0.2980995643126505 1104 5 0.3106015356367422 1104 6 0.25679795581446996 1104 7 0.17733373006690556 1104 8 0.16006690243931132 1104 9 0.2266013826541453 1104 10-11 0.2181702627610207 1104 12-13 0.27153866841739216 1104 14-15 0.3018654710393278 1104 16-17 0.2328955704968152 1104 18-19 0.31743554922393713 1104 20-21 0.3067844040463541 1104 22-23 0.40221437811975846 1104 24-25 0.3265268929554779 1104 26-27 0.4415289712351793 1104 28-29 0.4069088347664618 1104 30-31 0.35493922729230576 1104 32-33 0.2651700664072507 1104 34-35 0.23929762540823418 1104 36-37 0.2947019002307485 1104 38-39 0.05906330100901158 1104 40-41 0.19129186750270577 1104 42-43 0.18943675131983895 1104 44-45 0.24295900385627078 1104 46-47 0.4589653491071388 1104 48-49 0.2709612171342073 1104 50-51 0.33304893875918395 1104 52-53 0.30759072391212783 1104 54-55 0.28321512358426304 1104 56-57 0.29232167838179635 1104 58-59 0.035628254789571656 1104 60-61 0.11726893879187372 1104 62-63 0.09714140186662945 1104 64-65 0.1621114687707248 1104 66-67 0.11834220089276926 1104 68-69 -0.01972040159652977 1104 70-71 -0.05168213851587922 1104 72-73 0.07946885421458916 1104 74-75 -0.06272406927640617 1104 76-77 -0.030261923533096535 1104 78-79 -0.018510431111522507 1104 80-81 -0.11899185873813423 1104 82-83 -0.19782706941839479 1104 84-85 -0.108434409547435 1104 86-87 -0.06832185859026296 1104 88-89 -0.07299578983893795 1104 90-91 -0.036271978592932186 1104 92-93 -0.33685922161810566 1104 94-95 -0.35648677625167835 1104 96-97 -0.0792842828552267 1104 98-99 -0.10715447701484848 1104 100-101 -0.6172007929957353 1105 1 0.13962823644549616 1105 2 0.18839541821392203 1105 3 0.14712019531602039 1105 4 0.1151958426496833 1105 5 0.14053155821627428 1105 6 0.1352275456424863 1105 7 0.1328954742875581 1105 8 0.19805210970953624 1105 9 0.2263078761109938 1105 10-11 0.22231621332633011 1105 12-13 0.14944404190788418 1105 14-15 0.1974447164408346 1105 16-17 0.1714366655083026 1105 18-19 0.26072052906071974 1105 20-21 0.19122132198192077 1105 22-23 0.2513096953934948 1105 24-25 0.30969531656596416 1105 26-27 0.3386542452883816 1105 28-29 0.22619207813662712 1105 30-31 0.4419311538146502 1105 32-33 0.3676270574600835 1105 34-35 0.24254599201018578 1105 36-37 0.3760694222983503 1105 38-39 0.5168113276894388 1105 40-41 0.4527079068844557 1105 42-43 0.3327785212598613 1105 44-45 0.3844353008139123 1105 46-47 0.3031126921220917 1105 48-49 0.3619994385118659 1105 50-51 0.25242405650308797 1105 52-53 0.5216129648823653 1105 54-55 0.46712291065413325 1105 56-57 0.41667971814032967 1105 58-59 0.5160527335083032 1105 60-61 0.4343768208759613 1105 62-63 0.41330187213236513 1105 64-65 0.4838929306244282 1105 66-67 0.3963399948156834 1105 68-69 0.4041139678831627 1105 70-71 0.39226425490301864 1105 72-73 0.3735016614203772 1105 74-75 0.27143244752445383 1105 76-77 0.2847968733358073 1105 78-79 0.24966786250969264 1105 80-81 0.28210053166106164 1105 82-83 0.15188392902260617 1105 84-85 0.18620657051869216 1105 86-87 0.16521310691247848 1105 88-89 0.23986716854319923 1105 90-91 0.24833380799095295 1105 92-93 0.2670400469362839 1105 94-95 0.19901531105764292 1105 96-97 -0.24795839776235695 1105 98-99 -0.1531899544476083 1105 100-101 -0.36809291637223396 1106 1 0.11390363941981718 1106 2 0.14524075338933073 1106 3 0.15751016493754122 1106 4 0.14625932058866198 1106 5 0.17020696066869334 1106 6 0.1963973687658438 1106 7 0.1964326865279702 1106 8 0.13928490415320738 1106 9 0.150406969080926 1106 10-11 0.23546928634689834 1106 12-13 0.2206670208479835 1106 14-15 0.27604097268176275 1106 16-17 0.23874816813775368 1106 18-19 0.3071048412541231 1106 20-21 0.24779606911062046 1106 22-23 0.2809905032694644 1106 24-25 0.3559781372725368 1106 26-27 0.2976321132476798 1106 28-29 0.29275502280261634 1106 30-31 0.31479363499352075 1106 32-33 0.28610842421956306 1106 34-35 0.21961516018260596 1106 36-37 0.17340751215586891 1106 38-39 0.19735610864117348 1106 40-41 0.21148808545727604 1106 42-43 0.1185856311439153 1106 44-45 0.047064085029774105 1106 46-47 0.13422573220312017 1106 48-49 0.19693094433984726 1106 50-51 0.30845099191498804 1106 52-53 0.19994012422791485 1106 54-55 0.20398400357112934 1106 56-57 0.0964311133919793 1106 58-59 0.0646090007986686 1106 60-61 0.25501675964929404 1106 62-63 0.21132663717352074 1106 64-65 0.12091016572419022 1106 66-67 0.17702434753112328 1106 68-69 0.19691401828407606 1106 70-71 0.04403894864849178 1106 72-73 0.10453682612362769 1106 74-75 0.07617363770924612 1106 76-77 0.11283553217939613 1106 78-79 0.13592498322324076 1106 80-81 0.09899385954175344 1106 82-83 0.009862203693426608 1106 84-85 0.08552627000696589 1106 86-87 0.049426045557936504 1106 88-89 0.1390433447167645 1106 90-91 0.18202324068615994 1106 92-93 0.07101226902388902 1106 94-95 0.14611028733156672 1106 96-97 0.038798109528812574 1106 98-99 0.0810632080047533 1106 100-101 0.10148759666583373 1107 1 0.10813435715950703 1107 2 0.13311960007916213 1107 3 0.10257214990487995 1107 4 0.05395260142790903 1107 5 0.0677750208582708 1107 6 0.05556865867529126 1107 7 0.014632638116673036 1107 8 0.10752141579411045 1107 9 0.08754200874170692 1107 10-11 0.07836492873728673 1107 12-13 0.07201675975223765 1107 14-15 0.10324661124993639 1107 16-17 0.06650162346252131 1107 18-19 0.09963916897774538 1107 20-21 -0.0020851039809670624 1107 22-23 0.09653825623241374 1107 24-25 0.12933142094745875 1107 26-27 0.1872141066896873 1107 28-29 0.18369105897415494 1107 30-31 0.17284458258083646 1107 32-33 0.17511420823726098 1107 34-35 0.2834105194909142 1107 36-37 0.2664465105631919 1107 38-39 0.26543929987639814 1107 40-41 0.2220259881412332 1107 42-43 0.32279072899349615 1107 44-45 0.4413037338807584 1107 46-47 0.46528810136997123 1107 48-49 0.5838637042131651 1107 50-51 0.40893381102655724 1107 52-53 0.4378985332825138 1107 54-55 0.4159184661060138 1107 56-57 0.42234407692698994 1107 58-59 0.3760463760079489 1107 60-61 0.4191498299730725 1107 62-63 0.5044971044144049 1107 64-65 0.38987579388410865 1107 66-67 0.428371965970328 1107 68-69 0.4109972486939881 1107 70-71 0.5068341313112867 1107 72-73 0.3908991629264378 1107 74-75 0.3324768548680481 1107 76-77 0.41416080487030627 1107 78-79 0.5700035881441181 1107 80-81 0.4607672116573731 1107 82-83 0.44572244851369547 1107 84-85 0.3937924717526826 1107 86-87 0.4114441496573171 1107 88-89 0.44968985833976305 1107 90-91 0.5678254153129281 1107 92-93 0.5894703782483255 1107 94-95 0.6113590996465064 1107 96-97 0.7998608722396057 1107 98-99 0.8987438253547921 1107 100-101 0.7029235468507196 1108 1 0.09709077290330725 1108 2 0.14100206179663388 1108 3 0.17315035292869396 1108 4 0.24391274326892898 1108 5 0.20664088135818304 1108 6 0.1293533484326801 1108 7 0.0702404836983419 1108 8 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-0.6176375812691042 2316 82-83 -0.5493393041665051 2316 84-85 -0.5574894964544086 2316 86-87 -0.7009152318876168 2316 88-89 -0.8122884722306374 2316 90-91 -0.9629544342157779 2316 92-93 -1.0247258597893527 2316 94-95 -0.953810156773109 2316 96-97 -0.9201328099100365 2316 98-99 -1.1736010896756461 2316 100-101 -1.0211545599849785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6606.0 3 1528.0 4 1538.0 5 1736.0 6 2058.0 7 2466.0 8 3018.0 9 3840.0 10 4704.0 11 6057.0 12 8223.0 13 14148.0 14 11587.0 15 11224.0 16 13851.0 17 19985.0 18 16394.0 19 12119.0 20 12109.0 21 12736.0 22 13554.0 23 14726.0 24 16664.0 25 19313.0 26 21985.0 27 26026.0 28 31014.0 29 37249.0 30 45009.0 31 55527.0 32 71136.0 33 93883.0 34 133710.0 35 204189.0 36 341662.0 37 560867.0 38 553759.0 39 98646.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.435044254030394 18.489775599479582 14.181811477269356 23.893368669220663 2 15.548191427536795 20.05417443737165 39.85870040504925 24.538933730042313 3 19.314735755337914 23.74942393020892 29.72180375695431 27.21403655749886 4 13.414319224428642 16.193553854591563 36.14132303161032 34.25080388936947 5 14.532770580353002 36.51971287765159 33.54827000630019 15.39924653569521 6 32.6960731631993 34.803618208019394 17.3818531070828 15.1184555216985 7 29.00086544939039 32.34183572763676 20.58998396161656 18.067314861356287 8 26.23446456125995 34.386329692915126 19.930430641203184 19.448775104621735 9 26.619085899493054 17.090280130667978 20.489469528917642 35.801164440921326 10-11 25.582341231195503 25.822003941439753 26.92155176460835 21.67410306275639 12-13 26.29449207819954 23.300238095523493 27.48912507986129 22.916144746415682 14-15 23.09837934847923 24.845861513481722 25.816458538425152 26.239300599613895 16-17 22.843390804023656 27.98335050675237 26.42138176570449 22.751876923519486 18-19 22.693432193992997 26.551327454491187 28.590608263970108 22.16463208754571 20-21 23.467295110084482 25.79434980300209 28.32612409479954 22.41223099211389 22-23 23.095572530065596 25.579882874548694 28.38957499565425 22.934969599731463 24-25 23.076033056199734 25.821584047815787 28.349338206568277 22.753044689416203 26-27 22.788005204493803 26.05801959458744 28.41085286034335 22.743122340575407 28-29 22.54987804990143 26.423107495525034 28.51919849831332 22.50781595626022 30-31 22.817346263729654 25.931802329333493 28.22718943701946 23.023661969917388 32-33 22.62082915587921 26.05872238434762 28.21424875155893 23.106199708214238 34-35 23.36482030474015 26.131228584756744 27.825359974378323 22.678591136124783 36-37 23.371523947383366 25.71949314738329 27.996079459970495 22.912903445262852 38-39 22.890879960834003 26.010716474899752 28.166391280750176 22.93201228351607 40-41 22.997956002912545 26.036952418395572 28.13087599015511 22.834215588536765 42-43 23.09769152301053 26.067499054955444 27.609687368827412 23.225122053206608 44-45 23.016045969925948 26.108894432591832 27.705464828254904 23.169594769227313 46-47 23.20925633260641 26.431202358468987 27.26437984642341 23.095161462501192 48-49 23.27570706726119 26.325256960303093 27.24306979768264 23.155966174753072 50-51 23.025216458273675 26.617981322791977 27.229665203310343 23.127137015624005 52-53 23.452998745627205 26.55457645403198 26.91102220647205 23.081402593868763 54-55 22.90456742929276 26.3399465407928 27.17622736757449 23.579258662339953 56-57 23.084746517515782 26.65071085987837 27.068984090130847 23.195558532475 58-59 23.287488511716145 26.18203459061593 27.159887626431296 23.37058927123663 60-61 23.12357959329656 26.362407598383964 27.51541296645875 22.998599841860717 62-63 22.821422588434036 26.408419126608905 27.468473917941406 23.301684367015657 64-65 22.696180050370756 26.41474598890238 27.3388386630678 23.550235297659064 66-67 22.759503212995362 26.42935042863353 27.108356356889946 23.702790001481162 68-69 22.777511477668526 26.27316208764298 27.389073291678105 23.560253143010385 70-71 22.835819008161447 26.369242089801432 27.24592394222414 23.54901495981298 72-73 22.931988365393124 26.242930318594997 27.275197627962715 23.54988368804916 74-75 22.78752798536482 26.72104348908132 26.911991233343002 23.579437292210862 76-77 22.89107018773488 26.423221248086502 27.015394456743486 23.670314107435132 78-79 22.9220453306276 26.56930607862749 26.98062216573246 23.528026425012445 80-81 22.90774545897363 26.782511213011407 27.00465492693412 23.305088401080848 82-83 23.03868348889568 26.647680429281046 26.873304185165175 23.4403318966581 84-85 22.92297361309709 26.521286138564797 26.838846262418866 23.716893985919256 86-87 22.999893463280046 26.334472734524738 27.021885370903277 23.643748431291943 88-89 22.898370192485196 26.415165894275205 26.82960059843617 23.856863314803427 90-91 22.84137544601008 26.498261717178185 26.92904438496417 23.731318451847567 92-93 23.043769978759567 26.15204198552568 27.070901854277764 23.73328618143699 94-95 22.865074811263444 26.103293790824683 27.097425733115866 23.93420566479601 96-97 22.891178900760853 26.00501214100545 27.16179437618272 23.94201458205098 98-99 22.906466670988337 26.19261552257126 26.775619596113785 24.125298210326612 100-101 23.133753509128702 25.537808899136337 27.209328970118506 24.11910862161645 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2161.0 1 1215.5 2 321.5 3 395.0 4 372.0 5 380.5 6 629.0 7 1248.5 8 2003.5 9 2348.5 10 2300.0 11 2366.5 12 2600.5 13 2901.5 14 3241.0 15 3504.5 16 3734.5 17 3888.5 18 4069.0 19 4286.0 20 4522.5 21 4954.5 22 5545.0 23 6526.5 24 7918.0 25 9776.0 26 12512.5 27 15964.0 28 19149.0 29 22205.5 30 25760.5 31 29971.0 32 34675.0 33 37749.0 34 39658.5 35 41527.5 36 45013.5 37 49993.5 38 54107.5 39 56925.0 40 60246.5 41 66837.5 42 73500.0 43 78443.5 44 82360.0 45 86951.5 46 89653.0 47 93605.0 48 105068.5 49 107413.0 50 100764.5 51 97311.0 52 95016.0 53 94784.5 54 94048.5 55 88439.0 56 79115.0 57 70784.5 58 64534.0 59 57299.0 60 49070.5 61 41401.0 62 35147.0 63 28264.0 64 21687.0 65 16990.5 66 12916.0 67 10874.5 68 8530.5 69 5739.5 70 4343.0 71 3536.5 72 3052.5 73 2495.0 74 1954.0 75 1681.5 76 1277.0 77 792.5 78 458.0 79 316.0 80 217.5 81 157.0 82 117.5 83 80.5 84 55.5 85 43.0 86 36.0 87 28.0 88 20.0 89 14.0 90 12.5 91 10.5 92 9.5 93 7.0 94 6.0 95 4.5 96 2.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39726691857645025 2 0.11765105105464765 3 0.11968708892495204 4 0.13082541374485251 5 0.13385950939471788 6 0.07417565417697161 7 0.08459537739558819 8 0.08140159250099308 9 0.08116205863389843 10-11 0.07874675880736087 12-13 0.08335778574893259 14-15 0.07669075978146526 16-17 0.07848726378467502 18-19 0.08854768620264965 20-21 0.08677114335503111 22-23 0.09661199306150232 24-25 0.11028538464148765 26-27 0.11164274322169059 28-29 0.1042770768085306 30-31 0.09539436257043793 32-33 0.09357789741163695 34-35 0.3409964209648025 36-37 0.5338211839759828 38-39 0.5152972315873311 40-41 0.4870521964257555 42-43 0.5157563381659291 44-45 0.52493846973789 46-47 0.5221439079551193 48-49 0.5283717884995798 50-51 0.5277130703650695 52-53 0.5262359448513193 54-55 0.5213255005758793 56-57 0.4932601158146248 58-59 0.4833793437969711 60-61 0.43359622175246965 62-63 0.5063147115712823 64-65 0.5325636311737358 66-67 0.5419852966127915 68-69 0.5565569401943817 70-71 0.5519059909416275 72-73 0.5391308513632471 74-75 0.5432428494150383 76-77 0.5200479866180413 78-79 0.4941583678162297 80-81 0.5014042672958423 82-83 0.514379018430135 84-85 0.5085503609974988 86-87 0.5096681857106071 88-89 0.5204671708854569 90-91 0.5377335704718618 92-93 0.5439614510163222 94-95 0.5387715505626052 96-97 0.5395300744750715 98-99 0.5407277438105447 100-101 0.5178522595030072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2504865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.83309778868938 #Duplication Level Percentage of deduplicated Percentage of total 1 68.78980772910951 31.528499845134167 2 15.310432164138806 14.034490691321382 3 6.321006420373027 8.69133916163671 4 3.102522866493498 5.687929357265656 5 1.8220455347889013 4.175499558571228 6 1.1462077186965312 3.1520550274301207 7 0.7347795203644697 2.357405512699372 8 0.5201853898270783 1.9073366272153593 9 0.3732477204024246 1.5396389341748749 >10 1.6865330705580914 13.395676892976466 >50 0.11054842988144392 3.4938899469898095 >100 0.07596100870357547 6.666519354166121 >500 0.00488903757288587 1.5193006322555924 >1k 0.0016587806050851817 1.327392343063789 >5k 1.746084847458086E-4 0.5230261150992309 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7405 0.2956247143059606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5666 0.22619981515969922 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4272 0.1705481133713793 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3656 0.1459559696829969 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.9922311182438976E-5 0.0 16-17 0.0 0.0 0.0 3.9922311182438976E-5 0.0 18-19 0.0 0.0 0.0 5.9883466773658465E-5 0.0 20-21 0.0 0.0 0.0 1.1976693354731693E-4 0.0 22-23 0.0 0.0 0.0 2.594950226858533E-4 0.0 24-25 0.0 0.0 0.0 4.391454230068287E-4 0.0 26-27 0.0 0.0 0.0 9.581354683785353E-4 0.0 28-29 0.0 0.0 0.0 0.003313551828142435 0.0 30-31 0.0 0.0 0.0 0.011617392554089742 0.0 32-33 0.0 0.0 0.0 0.028125268228028258 0.0 34-35 0.0 0.0 0.0 0.05153970373652872 0.0 36-37 0.0 0.0 0.0 0.08980523900489647 0.0 38-39 0.0 0.0 0.0 0.1437203202567803 0.0 40-41 0.0 0.0 0.0 0.20526056294451 0.0 42-43 0.0 0.0 0.0 0.2711523375511255 0.0 44-45 0.0 0.0 0.0 0.3570651512157342 0.0 46-47 0.0 0.0 0.0 0.4605637429562072 0.0 48-49 0.0 0.0 0.0 0.56995087559609 0.0 50-51 0.0 0.0 0.0 0.6897178091434069 0.0 52-53 0.0 0.0 0.0 0.8101634219808254 0.0 54-55 0.0 0.0 0.0 0.9470769881809997 0.0 56-57 0.0 0.0 0.0 1.1122954730095236 0.0 58-59 0.0 0.0 0.0 1.2940218335119857 0.0 60-61 0.0 0.0 0.0 1.4910783615085044 0.0 62-63 0.0 0.0 0.0 1.6907697620430642 0.0 64-65 0.0 0.0 0.0 1.9133765691963438 0.0 66-67 0.0 0.0 0.0 2.174767901663363 0.0 68-69 0.0 0.0 0.0 2.459952931595116 0.0 70-71 0.0 0.0 0.0 2.775898102292938 0.0 72-73 0.0 0.0 0.0 3.11214376822703 0.0 74-75 0.0 0.0 0.0 3.4626816215644354 0.0 76-77 0.0 0.0 0.0 3.849409049988722 0.0 78-79 0.0 0.0 0.0 4.280570010759063 0.0 80-81 0.0 0.0 0.0 4.750774991865828 0.0 82-83 0.0 0.0 0.0 5.234593481085807 0.0 84-85 0.0 0.0 0.0 5.740808386879133 0.0 86-87 0.0 0.0 0.0 6.273910969253832 0.0 88-89 0.0 0.0 0.0 6.853063937577474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12810 0.0 26.467312 1 GGTATCA 9660 0.0 25.561537 1 GTACATG 8875 0.0 25.254244 1 TACATGG 9125 0.0 23.669006 2 ACATGGG 9235 0.0 22.515642 3 ATCAACG 15150 0.0 22.134888 3 TCAACGC 15315 0.0 21.927341 4 CAACGCA 15385 0.0 21.887835 5 TATCAAC 15490 0.0 21.832067 2 AACGCAG 15490 0.0 21.758734 6 GAGTACT 7665 0.0 21.36868 12-13 AGTACTT 7730 0.0 20.637835 12-13 ATACCGT 235 1.17492164E-7 20.143606 6 CATGGGG 5230 0.0 20.105183 4 GTACTTT 8135 0.0 19.72775 14-15 ACGCAGA 17065 0.0 19.69505 7 CGCAGAG 17465 0.0 19.243975 8 AGAGTAC 13105 0.0 19.162361 10-11 GTATAGA 735 0.0 18.089703 1 GTCTACG 290 5.2385076E-8 18.01173 1 >>END_MODULE