FastQCFastQC Report
Fri 27 May 2016
SRR939090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504865
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75300.30061500320376544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61470.2454024468384524No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45040.17981008956570513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38620.15417996578657933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136250.025.9866751
GTACATG92800.024.5458641
TACATGG94350.023.9398632
GGTATCA100800.023.779671
ACCGTAT1000.001663903623.7261168
ACATGGG95450.022.7691173
TAAGGTG11200.022.0313935
GAGTACT79950.021.9612612-13
ATCAACG161600.021.6706373
TCAACGC161550.021.647974
GTGTAAG8150.021.6342831
CAACGCA162450.021.5572455
AACGCAG164150.021.36296
TATCAAC164850.021.2721862
AGTACTT81550.021.03576512-13
GTACTTT86100.020.50282714-15
AGAGTAC139600.019.54573810-11
ACGCAGA181300.019.2897387
CATGGGG52250.019.1625294
TACCGTA1250.006068245518.9808947
Produced by FastQC (version 0.11.5)