##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939090_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2504865 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54298654817725 34.0 31.0 34.0 31.0 34.0 2 32.758855666872265 34.0 31.0 34.0 31.0 34.0 3 32.86358466424338 34.0 31.0 34.0 31.0 34.0 4 36.30225940320137 37.0 37.0 37.0 35.0 37.0 5 36.261096705810495 37.0 37.0 37.0 35.0 37.0 6 36.29670620971589 37.0 37.0 37.0 35.0 37.0 7 36.24900902843068 37.0 37.0 37.0 35.0 37.0 8 36.226654131060954 37.0 37.0 37.0 35.0 37.0 9 37.98314360254944 39.0 38.0 39.0 35.0 39.0 10-11 37.908215013583565 39.0 38.0 39.0 35.0 39.0 12-13 37.825640902803144 39.0 38.0 39.0 35.0 39.0 14-15 39.265673998399116 40.5 39.0 41.0 36.0 41.0 16-17 39.3522511193218 41.0 39.0 41.0 36.0 41.0 18-19 39.37526473482603 41.0 39.0 41.0 36.0 41.0 20-21 39.337731973579416 41.0 39.0 41.0 36.0 41.0 22-23 39.27081459479852 41.0 39.0 41.0 36.0 41.0 24-25 39.19315332363222 41.0 39.0 41.0 36.0 41.0 26-27 39.054930305625255 40.5 39.0 41.0 35.0 41.0 28-29 38.90931167947175 40.0 38.0 41.0 35.0 41.0 30-31 38.72406337267677 40.0 38.0 41.0 35.0 41.0 32-33 38.64142698309091 40.0 38.0 41.0 35.0 41.0 34-35 38.53797050938873 40.0 38.0 41.0 34.5 41.0 36-37 38.389873106933905 40.0 38.0 41.0 34.0 41.0 38-39 38.2581917588373 40.0 38.0 41.0 34.0 41.0 40-41 38.07243703752498 40.0 38.0 41.0 33.5 41.0 42-43 37.881403389005 40.0 38.0 41.0 33.0 41.0 44-45 37.62284953480527 40.0 37.0 41.0 33.0 41.0 46-47 37.31694901721251 40.0 37.0 41.0 32.5 41.0 48-49 36.97452138139181 40.0 36.5 41.0 31.5 41.0 50-51 36.70483179732241 39.5 36.0 41.0 31.0 41.0 52-53 36.43149511051494 39.0 35.5 41.0 30.5 41.0 54-55 36.170922784261826 39.0 35.0 40.0 30.0 41.0 56-57 35.92336592990041 39.0 35.0 40.0 29.5 41.0 58-59 35.57867709437434 38.0 35.0 40.0 29.0 41.0 60-61 35.195508540380416 38.0 34.5 40.0 28.0 41.0 62-63 34.796984468224835 37.0 34.0 40.0 28.0 41.0 64-65 34.35102450631072 37.0 34.0 39.5 26.5 41.0 66-67 33.928819517219495 36.0 33.5 39.0 26.0 40.5 68-69 33.44846648422171 35.5 33.0 39.0 26.0 40.0 70-71 32.94058542077118 35.0 33.0 38.0 25.0 40.0 72-73 32.382829214348874 35.0 32.0 37.0 23.5 39.5 74-75 31.85951957490723 35.0 31.5 37.0 22.0 39.0 76-77 29.86094540025111 32.5 29.0 35.0 19.5 37.0 78-79 30.86215804045328 34.0 30.5 35.0 20.0 37.0 80-81 30.89557640830943 34.0 31.0 35.0 20.0 37.0 82-83 30.53636683014853 34.0 31.0 35.0 18.0 37.0 84-85 30.098296714593403 34.0 30.5 35.0 14.5 36.0 86-87 29.428377976457814 34.0 29.5 35.0 7.0 36.0 88-89 28.77834194657197 34.0 29.0 35.0 2.0 35.0 90-91 28.244497607655504 33.0 28.5 35.0 2.0 35.0 92-93 27.817850462999004 33.0 27.0 35.0 2.0 35.0 94-95 27.303753296085816 33.0 26.5 34.5 2.0 35.0 96-97 26.53729662077597 32.5 25.0 34.0 2.0 35.0 98-99 25.345763943366208 32.0 21.5 34.0 2.0 35.0 100-101 19.371469919536583 25.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.056278801288257796 1101 2 0.1117537387414913 1101 3 0.17641193177487224 1101 4 0.14672296410999763 1101 5 0.07756567085502297 1101 6 0.1739283393686506 1101 7 0.2107788311977572 1101 8 0.2145668641252385 1101 9 0.2049615117695751 1101 10-11 0.2469646176878797 1101 12-13 0.2447915506849725 1101 14-15 0.2840360287886341 1101 16-17 0.22025792406759592 1101 18-19 0.17918761100601444 1101 20-21 0.24331249198476712 1101 22-23 0.2785406928802061 1101 24-25 0.2698008422267506 1101 26-27 0.2644326341864769 1101 28-29 0.22963846985830116 1101 30-31 0.2319201917584408 1101 32-33 0.3683511091805869 1101 34-35 0.3068247768121921 1101 36-37 0.1940089973660193 1101 38-39 0.16341317976615244 1101 40-41 0.2629716416210073 1101 42-43 0.2872854931243083 1101 44-45 0.38171577098760423 1101 46-47 0.42012578580497717 1101 48-49 0.27778425191424816 1101 50-51 0.29825847859688537 1101 52-53 0.3114293428200199 1101 54-55 0.2372615895119452 1101 56-57 0.14544748084347248 1101 58-59 0.2020159739922036 1101 60-61 0.2370495981410059 1101 62-63 0.1350025216687598 1101 64-65 0.219637033490649 1101 66-67 0.19602716246437524 1101 68-69 0.280279637540481 1101 70-71 0.287993640106869 1101 72-73 0.1876735554446256 1101 74-75 0.1460477021145543 1101 76-77 0.3643513176259461 1101 78-79 0.4322973443734419 1101 80-81 0.48494557315408926 1101 82-83 0.3555483888693267 1101 84-85 0.26575506356277856 1101 86-87 0.43895858807408317 1101 88-89 0.5116177925629692 1101 90-91 0.7392902637818111 1101 92-93 0.5905156102161264 1101 94-95 0.30515805847198507 1101 96-97 0.3710386778998256 1101 98-99 0.25886255305601225 1101 100-101 -0.5516649270122933 1102 1 0.1531905840786436 1102 2 0.16708766371453265 1102 3 0.12611219774636595 1102 4 0.12229940216962376 1102 5 0.17453094983208217 1102 6 0.15572054597313922 1102 7 0.1481825214135526 1102 8 0.18115816258202244 1102 9 0.2566281732568072 1102 10-11 0.18951504061852376 1102 12-13 0.21725899459278253 1102 14-15 0.22837446741918654 1102 16-17 0.197013054041129 1102 18-19 0.24270467673350993 1102 20-21 0.24366754240215016 1102 22-23 0.18159073023874583 1102 24-25 0.20842784150799076 1102 26-27 0.2049258956647364 1102 28-29 0.19763998107008263 1102 30-31 0.3281531723129092 1102 32-33 0.21537132718753327 1102 34-35 0.23235486630842672 1102 36-37 0.27986482758466025 1102 38-39 0.3813581981168994 1102 40-41 0.3031963344840847 1102 42-43 0.28873222091129236 1102 44-45 0.28824072973339554 1102 46-47 0.31845339734008604 1102 48-49 0.35103151928019116 1102 50-51 0.331026113803496 1102 52-53 0.30157583358116113 1102 54-55 0.27751680405829404 1102 56-57 0.19711392035483044 1102 58-59 0.04907903334366637 1102 60-61 -0.0999282091546263 1102 62-63 0.03941604585990888 1102 64-65 0.10893166962113554 1102 66-67 -0.01478810166250355 1102 68-69 0.009686047404734666 1102 70-71 0.05275991948482073 1102 72-73 -0.040750389182157676 1102 74-75 0.05427022138982451 1102 76-77 0.14095523763116802 1102 78-79 0.25485704145627963 1102 80-81 0.3460509375563525 1102 82-83 0.39392524353328184 1102 84-85 0.3147206115746073 1102 86-87 0.4300509180527712 1102 88-89 0.37554125172851016 1102 90-91 0.5856865075947901 1102 92-93 0.5176147362083015 1102 94-95 0.6135262827137957 1102 96-97 0.4536527723914041 1102 98-99 0.546262158915642 1102 100-101 -0.1631169067485594 1103 1 0.05852720650418064 1103 2 0.07573505109943568 1103 3 0.08566760192751843 1103 4 0.06843391507510432 1103 5 0.10207152812441933 1103 6 0.1336802122528553 1103 7 0.07270168759509232 1103 8 0.10405725422075562 1103 9 0.12605188744507956 1103 10-11 0.024144828288648057 1103 12-13 0.14205029023905524 1103 14-15 0.0731713262184428 1103 16-17 0.09091668662501462 1103 18-19 0.1485817904733011 1103 20-21 0.2323208847720224 1103 22-23 0.11644737140332495 1103 24-25 0.17877240391347726 1103 26-27 0.16467086471431713 1103 28-29 0.06505404930224046 1103 30-31 0.12255710113899454 1103 32-33 0.17142295686411302 1103 34-35 0.20101239826641404 1103 36-37 0.13846407561172214 1103 38-39 0.20613459553614177 1103 40-41 0.2904361752711182 1103 42-43 0.3074672796258042 1103 44-45 0.28248209134522284 1103 46-47 0.28605319823777364 1103 48-49 0.2119328878502742 1103 50-51 0.2763160350237399 1103 52-53 0.14126854257830956 1103 54-55 0.10969688831508506 1103 56-57 0.09857194438489358 1103 58-59 -0.02671558899919546 1103 60-61 0.11023744104533506 1103 62-63 0.07681371743064602 1103 64-65 0.17623817782118323 1103 66-67 0.16587582964375258 1103 68-69 -0.019179698040545645 1103 70-71 0.06275098916029975 1103 72-73 0.053712551262819375 1103 74-75 -0.02522785906404934 1103 76-77 0.09951394611850617 1103 78-79 0.046982962476729284 1103 80-81 0.1519144448228964 1103 82-83 0.0798603914649263 1103 84-85 -0.14857807979268856 1103 86-87 -0.037757178143273507 1103 88-89 0.07775755676152585 1103 90-91 0.20012835800625695 1103 92-93 0.3166938449326331 1103 94-95 0.11628751408239779 1103 96-97 -0.09157938590527337 1103 98-99 -0.30392970958038035 1103 100-101 -0.9299557785480239 1104 1 0.10020503972219075 1104 2 0.10637748919207013 1104 3 0.11981593108659183 1104 4 0.09905158372657752 1104 5 0.06835970960889881 1104 6 0.04616815237740468 1104 7 0.08391960507793073 1104 8 0.09999872037676738 1104 9 0.14104897196003918 1104 10-11 0.10506202445287016 1104 12-13 0.09953882320596819 1104 14-15 0.09731523748374826 1104 16-17 0.07167641411243864 1104 18-19 0.18597684519345847 1104 20-21 0.21482787300288209 1104 22-23 0.19833047425903771 1104 24-25 0.15736368864347838 1104 26-27 0.18073171551619538 1104 28-29 0.2333501188385867 1104 30-31 0.2917434493599842 1104 32-33 0.2709744878592133 1104 34-35 0.29541340820991735 1104 36-37 0.3866881878911954 1104 38-39 0.36324249122187524 1104 40-41 0.25215906450868175 1104 42-43 0.28903769874099083 1104 44-45 0.26962390898366095 1104 46-47 0.32865135831495706 1104 48-49 0.28770057810394434 1104 50-51 0.28512421072597505 1104 52-53 0.20699993950982787 1104 54-55 0.28151762902695054 1104 56-57 0.3170271474293358 1104 58-59 0.32976590376163273 1104 60-61 0.37455184321660084 1104 62-63 0.3545509234052062 1104 64-65 0.351447357782142 1104 66-67 0.303428962125345 1104 68-69 0.21262917770294365 1104 70-71 0.039770023460697246 1104 72-73 -0.040302096004090515 1104 74-75 -0.03599474938658531 1104 76-77 -0.016694424978069833 1104 78-79 0.23168168080026774 1104 80-81 0.3354094089112678 1104 82-83 0.30018649943045617 1104 84-85 0.24425226334122385 1104 86-87 0.28519278514922775 1104 88-89 0.44046825943402723 1104 90-91 0.5366295404425294 1104 92-93 0.4468136072109665 1104 94-95 0.47282260628968587 1104 96-97 0.14361346246677087 1104 98-99 -0.18013366684375143 1104 100-101 -0.6582402876583053 1105 1 -7.229870128426796E-4 1105 2 0.020893367213481895 1105 3 0.0189861949148451 1105 4 0.019926315133957928 1105 5 -0.03901317475498445 1105 6 0.06605560968243651 1105 7 0.02767419538375293 1105 8 0.03513240919430416 1105 9 0.06384856023178997 1105 10-11 0.07423155110191004 1105 12-13 0.08630099553662518 1105 14-15 0.09568047429574733 1105 16-17 0.0749541313687132 1105 18-19 0.11205005528134393 1105 20-21 0.10077669092234487 1105 22-23 0.07015002370601309 1105 24-25 -0.037560832394795796 1105 26-27 0.009348466958478241 1105 28-29 0.0970197424130319 1105 30-31 0.08395105541140424 1105 32-33 0.17313442298743809 1105 34-35 0.19261240018558823 1105 36-37 0.20246982994426332 1105 38-39 0.19338206382174405 1105 40-41 0.28774093099187326 1105 42-43 0.21721937792605672 1105 44-45 0.10235453004350603 1105 46-47 0.2455753551369 1105 48-49 0.2820468229948858 1105 50-51 0.22170905049870981 1105 52-53 0.22038861589087588 1105 54-55 0.20195892635317136 1105 56-57 0.20576018206856617 1105 58-59 0.22060535977797002 1105 60-61 0.16792050654684942 1105 62-63 -0.010017649279305374 1105 64-65 0.17673656411314198 1105 66-67 0.20519436985371442 1105 68-69 0.2055331450923248 1105 70-71 0.2198704163499201 1105 72-73 -0.01833415510948555 1105 74-75 -0.12374501490762313 1105 76-77 -0.41602918898515995 1105 78-79 -0.31893113355889113 1105 80-81 -0.09345893907009284 1105 82-83 -0.06929864913846373 1105 84-85 8.09063000481558E-4 1105 86-87 0.04954798540260086 1105 88-89 0.02755028563938211 1105 90-91 -0.060204411302265726 1105 92-93 -0.19761481951205084 1105 94-95 -0.13937265883545535 1105 96-97 -0.26604317583214865 1105 98-99 -0.3410162068903304 1105 100-101 -0.8803858341401849 1106 1 -0.035603998729186515 1106 2 -0.005207559126993999 1106 3 -0.004930008345482406 1106 4 0.011312120342623189 1106 5 0.04720649942099442 1106 6 0.018737277579923273 1106 7 0.050017793181467596 1106 8 0.04098927879796577 1106 9 0.08134405229495201 1106 10-11 0.05443390779200996 1106 12-13 0.0724536051819129 1106 14-15 0.05295143997652474 1106 16-17 -0.0025911427813198884 1106 18-19 0.010940116795069343 1106 20-21 0.10712247176559231 1106 22-23 0.15706159209524628 1106 24-25 0.11968918260991046 1106 26-27 0.07289257577375707 1106 28-29 0.18054565172642612 1106 30-31 0.12989799428277138 1106 32-33 0.0011478254555257195 1106 34-35 -0.14052944446552118 1106 36-37 -0.11749452408339067 1106 38-39 0.0096952406413493 1106 40-41 0.08881305015275842 1106 42-43 0.04576130034070758 1106 44-45 0.20978667054276912 1106 46-47 0.09788315411925907 1106 48-49 0.08571000397107298 1106 50-51 0.04157200623648549 1106 52-53 0.021060920103643355 1106 54-55 -0.023519835737729977 1106 56-57 -0.008758431414193524 1106 58-59 -0.12210082264724775 1106 60-61 0.15168130947218827 1106 62-63 0.06313292233491552 1106 64-65 0.09100179370757644 1106 66-67 0.06322334366946336 1106 68-69 -0.04272826566357679 1106 70-71 -0.06613790576560774 1106 72-73 -0.1148222700742707 1106 74-75 -0.07016981185941162 1106 76-77 -0.182336047051038 1106 78-79 -0.03707128761719147 1106 80-81 0.028847249468846314 1106 82-83 0.0017703684276710874 1106 84-85 -0.15543647519517378 1106 86-87 -0.05229410838262183 1106 88-89 -0.10420570161276643 1106 90-91 0.1431567556131128 1106 92-93 0.10211032402058251 1106 94-95 -0.08788199213796943 1106 96-97 -0.3775385172890289 1106 98-99 -0.20166774309306135 1106 100-101 -0.9744930464290604 1107 1 0.0892432334439448 1107 2 0.046156933182452065 1107 3 0.0022323116373641483 1107 4 -0.023438168840286266 1107 5 -0.0956573180234912 1107 6 -0.02914138581547121 1107 7 -0.006637978248207332 1107 8 -0.03486748342799473 1107 9 0.024137139650747486 1107 10-11 0.020927594826098073 1107 12-13 0.03517106740932974 1107 14-15 0.06665359765754886 1107 16-17 0.015306930057370494 1107 18-19 0.021644511926119492 1107 20-21 0.04901490057322633 1107 22-23 0.08361703857264757 1107 24-25 0.055267836858561736 1107 26-27 0.017787798743349015 1107 28-29 0.12902625764751718 1107 30-31 0.059902431086356955 1107 32-33 0.04365209828245753 1107 34-35 0.017472337084534217 1107 36-37 0.08354551011719735 1107 38-39 0.014493871036563633 1107 40-41 0.18240516647931315 1107 42-43 0.106296085222219 1107 44-45 0.05950203404888299 1107 46-47 0.15996848414898324 1107 48-49 0.08170475096815011 1107 50-51 0.10879362376676482 1107 52-53 0.13588949398127426 1107 54-55 -0.01565003516746799 1107 56-57 -0.02915362140875999 1107 58-59 -0.051911003287514745 1107 60-61 -0.11088994848651623 1107 62-63 -0.0394192370622406 1107 64-65 -0.006181995085476899 1107 66-67 -0.08456139031190446 1107 68-69 -0.20946240984118702 1107 70-71 -0.33947807130923735 1107 72-73 -0.2704523653676034 1107 74-75 -0.3514275782873071 1107 76-77 -0.31799603371367624 1107 78-79 -0.08194392224291036 1107 80-81 -0.1825917789843814 1107 82-83 -0.459543274051498 1107 84-85 -0.36190364204001213 1107 86-87 -0.23133171195520674 1107 88-89 -0.31806825989635357 1107 90-91 0.02945337596917952 1107 92-93 0.13454032126936966 1107 94-95 0.08998657029633605 1107 96-97 -0.2364984794944327 1107 98-99 0.09741989986087418 1107 100-101 -0.44205145850262895 1108 1 -0.03118052354248846 1108 2 0.06934669403349858 1108 3 0.0691948844451673 1108 4 0.08621990286976455 1108 5 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-1.434688096148971 2316 94-95 -1.053742468235864 2316 96-97 -0.9035569583093199 2316 98-99 -0.6649148222681376 2316 100-101 0.6528357368835138 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 18.0 5 66.0 6 87.0 7 205.0 8 582.0 9 1041.0 10 1839.0 11 2940.0 12 4589.0 13 6254.0 14 7893.0 15 10304.0 16 13842.0 17 19627.0 18 19395.0 19 14202.0 20 13308.0 21 14095.0 22 14685.0 23 15631.0 24 18295.0 25 21072.0 26 25153.0 27 29795.0 28 36394.0 29 44232.0 30 54643.0 31 67557.0 32 85871.0 33 113450.0 34 162321.0 35 248654.0 36 409995.0 37 619596.0 38 389934.0 39 17291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.82583984715654 18.86878998257857 14.587930097772503 23.717440072492387 2 15.831352188640905 20.40525138081294 39.194168148782474 24.56922828176369 3 19.31788739113685 23.853501086884922 29.83809506699962 26.990516454978614 4 13.597539188738713 16.609797334387284 35.89526780884399 33.897395668030015 5 14.52952554329275 36.64983941250327 33.5143810145457 15.306254029658284 6 32.794302287748046 35.067997676521486 17.073574823393674 15.064125212336792 7 28.98599325712164 32.484784609150594 20.34245358532296 18.186768548404807 8 26.373676824898745 34.601824848844146 19.658344860900687 19.36615346535642 9 26.500669696770085 16.98822092208562 20.342573352256508 36.16853602888778 10-11 25.603016529832946 25.93924223461145 26.745393464318433 21.712347771237173 12-13 26.294850221469023 23.352975110435093 27.391595954272987 22.9605787138229 14-15 23.035661495154688 24.921626871448506 25.752716386186847 26.28999524720996 16-17 22.796563242698127 28.076140119602222 26.41786599584878 22.709430641850876 18-19 22.65409513087532 26.550931886548778 28.64228211899643 22.152690863579476 20-21 23.42168140798007 25.810173402558622 28.376778788477626 22.391366400983685 22-23 23.040523141965735 25.592337311591645 28.488900599433503 22.87823894700912 24-25 23.06862690694062 25.846762876851948 28.45409110198841 22.630519114219023 26-27 22.760598153287958 26.05103210826038 28.558022753332036 22.63034698511963 28-29 22.505393506393958 26.47666699669124 28.571631034658967 22.446308462255836 30-31 22.716633431342608 25.98171957370956 28.372187722691645 22.92945927225619 32-33 22.623439311787124 26.009450810612712 28.350834945363314 23.016274932236854 34-35 23.317537512993773 26.11516847932306 27.942870729473835 22.624423278209335 36-37 23.3090142380516 25.717083864939173 28.10918035863677 22.864721538372457 38-39 22.861409731296735 26.004929437566947 28.294093160180765 22.83956767095555 40-41 22.94434317698307 25.99202241756162 28.255418385890046 22.808216019565258 42-43 23.06613238054571 26.009157764726055 27.768185621107616 23.15652423362062 44-45 22.96031467990542 26.05950452487107 27.798847597631315 23.181333197592192 46-47 23.201438561693823 26.417561376023862 27.337742601580057 23.043257460702257 48-49 23.246624557895835 26.284292890841243 27.377261822680847 23.09182072858208 50-51 22.99925823783179 26.593610944545095 27.313894746423532 23.093236071199584 52-53 23.37179848015761 26.587121461635654 26.942549798092912 23.09853026011382 54-55 22.905465963235542 26.295528900759123 27.189748748934573 23.609256387070758 56-57 23.07138308850976 26.62516742419252 27.0810203344292 23.222429152868518 58-59 23.261477913695646 26.204581843456708 27.220103308758723 23.313836934088915 60-61 23.07826050851944 26.366166414018522 27.548954986843267 23.006618090618776 62-63 22.783465128836216 26.44605267228981 27.409911460968868 23.360570737905107 64-65 22.63852835802066 26.39240991474748 27.331748638810215 23.637313088421642 66-67 22.73403697881578 26.435391870649 27.091129575567034 23.739441574968186 68-69 22.713480728185612 26.322150030382325 27.322311324182174 23.642057917249893 70-71 22.763225526351246 26.40079046255034 27.157997694484227 23.677986316614184 72-73 22.87153199873047 26.200673489389647 27.204859343717125 23.722935168162756 74-75 22.667528988588206 26.683933066253076 26.817792575647793 23.83074536951093 76-77 22.69902807479766 26.324201383109724 26.998472868794792 23.97829767329782 78-79 22.74136311501838 26.43048017576731 26.985173376563093 23.84298333265121 80-81 22.730237878040846 26.66617428952973 26.95351627140094 23.650071561028483 82-83 22.813784375605074 26.461146608699472 26.889852347332095 23.835216668363365 84-85 22.624592542911497 26.316907298397318 26.843901767161103 24.21459839153008 86-87 22.69299340452751 26.11849869573263 26.99425818619111 24.194249713548754 88-89 22.514968907607997 26.26255276526151 26.775437421236713 24.447040905893775 90-91 22.486360382576812 26.21719190160432 26.905562349945168 24.390885365873704 92-93 22.55658488581221 25.955969682996887 27.01766362658267 24.469781804608232 94-95 22.292359069251237 25.855724759617782 27.09549217223283 24.756423998898143 96-97 22.255949921452853 25.684637695045442 27.17463815415202 24.884774229349684 98-99 22.159493081652034 25.860456927550075 26.85035217523048 25.129697815567408 100-101 22.46440065528704 24.753653552608704 27.56355503798321 25.218390754121046 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 365.0 1 263.0 2 220.0 3 289.5 4 284.5 5 290.0 6 456.5 7 996.0 8 1652.0 9 1988.0 10 1968.5 11 1884.5 12 2028.0 13 2339.5 14 2564.5 15 2729.0 16 2957.0 17 3069.0 18 3276.5 19 3574.0 20 3894.5 21 4430.0 22 5238.5 23 6381.0 24 7740.5 25 9519.5 26 12328.0 27 15767.5 28 18831.0 29 21926.0 30 25538.5 31 29762.0 32 34748.0 33 37797.0 34 39461.0 35 41566.5 36 45105.0 37 50222.5 38 54588.5 39 57066.5 40 59919.0 41 66777.5 42 73622.5 43 78471.0 44 82794.5 45 87346.0 46 89998.0 47 94443.0 48 105487.0 49 107028.5 50 101092.0 51 98576.0 52 96319.0 53 96107.0 54 95487.0 55 89974.5 56 80646.0 57 72176.5 58 65532.5 59 57950.0 60 49842.5 61 41897.0 62 35211.5 63 28418.0 64 21856.5 65 16994.0 66 12714.5 67 10552.0 68 8133.5 69 5362.0 70 3989.0 71 3306.0 72 2923.5 73 2436.0 74 2005.5 75 1729.0 76 1177.0 77 622.0 78 375.5 79 266.5 80 146.0 81 85.0 82 57.0 83 23.5 84 19.0 85 15.5 86 11.0 87 10.5 88 7.0 89 2.0 90 1.5 91 1.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4315601838821653 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.184073792399989E-4 16-17 0.002475183293311216 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.984462236487795E-5 26-27 0.0035530856952370685 28-29 3.9922311182438976E-5 30-31 0.0 32-33 1.9961155591219488E-5 34-35 0.011956732199140473 36-37 0.02634872538040972 38-39 0.020859407592824362 40-41 0.008842791926910232 42-43 0.00856333574863316 44-45 0.0038924253402878002 46-47 0.005629045876723895 48-49 0.0032736295169599958 50-51 7.585239124663405E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0017366205364360953 60-61 0.0025550279156760945 62-63 0.0014970866693414615 64-65 0.0028344840939531673 66-67 0.0020959213370780458 68-69 0.004750755030710238 70-71 9.980577795609743E-5 72-73 0.0 74-75 0.0 76-77 0.0982288466643911 78-79 0.01666756491866827 80-81 3.9922311182438974E-4 82-83 0.0 84-85 0.0 86-87 0.10755070632549059 88-89 0.4169685791449838 90-91 0.42191894573160627 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.596892447297559E-4 100-101 3.393396450507313E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2504865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.68899002903463 #Duplication Level Percentage of deduplicated Percentage of total 1 67.52115247176826 30.174521095597783 2 16.017070208328583 14.31573381668687 3 6.52046349726976 8.741787846425177 4 3.2749147391303848 5.854105284917452 5 1.8541488495683691 4.143001972535344 6 1.1219633377522544 3.0083645048251744 7 0.783713260892039 2.451634787113858 8 0.5254849469917832 1.878671324521887 9 0.3883852278864833 1.5620889218799054 >10 1.7889074220015917 13.854293771694376 >50 0.11630595122186527 3.6163595277888607 >100 0.07988679695943747 6.768751994784174 >500 0.005635379817079833 1.6878838406335548 >1k 0.0017890094657386092 1.396069895089525 >5k 1.7890094657386092E-4 0.546731415506226 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7530 0.30061500320376544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6147 0.2454024468384524 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4504 0.17981008956570513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3862 0.15417996578657933 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.9961155591219488E-5 0.0 20-21 0.0 0.0 0.0 7.984462236487795E-5 0.0 22-23 0.0 0.0 0.0 2.1957271150341436E-4 0.0 24-25 0.0 0.0 0.0 3.9922311182438974E-4 3.9922311182438976E-5 26-27 0.0 0.0 0.0 9.381743127873159E-4 3.9922311182438976E-5 28-29 0.0 0.0 0.0 0.003313551828142435 3.9922311182438976E-5 30-31 0.0 0.0 0.0 0.011657314865272179 3.9922311182438976E-5 32-33 0.0 0.0 0.0 0.028484569028670206 3.9922311182438976E-5 34-35 0.0 0.0 0.0 0.05207865493749164 3.9922311182438976E-5 36-37 0.0 0.0 0.0 0.09070349100650135 3.9922311182438976E-5 38-39 0.0 0.0 0.0 0.14525732923730422 3.9922311182438976E-5 40-41 0.0 0.0 0.0 0.20739640659277048 3.9922311182438976E-5 42-43 0.0 0.0 0.0 0.2738670547115314 3.9922311182438976E-5 44-45 0.0 0.0 0.0 0.3606381980665625 3.9922311182438976E-5 46-47 0.0 0.0 0.0 0.46475558563036334 3.9922311182438976E-5 48-49 0.0 0.0 0.0 0.5748812810271212 3.9922311182438976E-5 50-51 0.0 0.0 0.0 0.6961053789325972 3.9922311182438976E-5 52-53 0.0 0.0 0.0 0.817229671060117 3.9922311182438976E-5 54-55 0.0 0.0 0.0 0.9548418777059842 3.9922311182438976E-5 56-57 0.0 0.0 0.0 1.1212181095587987 3.9922311182438976E-5 58-59 0.0 0.0 0.0 1.3048008575312442 3.9922311182438976E-5 60-61 0.0 0.0 0.0 1.5030151325520538 3.9922311182438976E-5 62-63 0.0 0.0 0.0 1.7039241635776778 3.9922311182438976E-5 64-65 0.0 0.0 0.0 1.928307513578576 3.9922311182438976E-5 66-67 0.0 0.0 0.0 2.191056204625798 3.9922311182438976E-5 68-69 0.0 0.0 0.0 2.4787363790064534 3.9922311182438976E-5 70-71 0.0 0.0 0.0 2.7971966553087695 3.9922311182438976E-5 72-73 0.0 0.0 0.0 3.13669598960423 3.9922311182438976E-5 74-75 0.0 0.0 0.0 3.490607278236552 3.9922311182438976E-5 76-77 0.0 0.0 0.0 3.8790713271972743 3.9922311182438976E-5 78-79 0.0 0.0 0.0 4.312348170460284 3.9922311182438976E-5 80-81 0.0 0.0 0.0 4.783491325879838 3.9922311182438976E-5 82-83 0.0 0.0 0.0 5.268447600968516 3.9922311182438976E-5 84-85 0.0 0.0 0.0 5.774742351384206 3.9922311182438976E-5 86-87 0.0 0.0 0.0 6.308064506470409 3.9922311182438976E-5 88-89 0.0 0.0 0.0 6.88753685328351 3.9922311182438976E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13625 0.0 25.986675 1 GTACATG 9280 0.0 24.545864 1 TACATGG 9435 0.0 23.939863 2 GGTATCA 10080 0.0 23.77967 1 ACCGTAT 100 0.0016639036 23.726116 8 ACATGGG 9545 0.0 22.769117 3 TAAGGTG 1120 0.0 22.031393 5 GAGTACT 7995 0.0 21.96126 12-13 ATCAACG 16160 0.0 21.670637 3 TCAACGC 16155 0.0 21.64797 4 GTGTAAG 815 0.0 21.634283 1 CAACGCA 16245 0.0 21.557245 5 AACGCAG 16415 0.0 21.3629 6 TATCAAC 16485 0.0 21.272186 2 AGTACTT 8155 0.0 21.035765 12-13 GTACTTT 8610 0.0 20.502827 14-15 AGAGTAC 13960 0.0 19.545738 10-11 ACGCAGA 18130 0.0 19.289738 7 CATGGGG 5225 0.0 19.162529 4 TACCGTA 125 0.0060682455 18.980894 7 >>END_MODULE