##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939089_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 263998 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.998386351411753 33.0 31.0 34.0 30.0 34.0 2 32.03016689520375 34.0 31.0 34.0 30.0 34.0 3 32.034409351586 34.0 31.0 34.0 30.0 34.0 4 35.49546208683399 37.0 35.0 37.0 33.0 37.0 5 35.55511405389435 37.0 35.0 37.0 35.0 37.0 6 35.70098258320139 37.0 36.0 37.0 35.0 37.0 7 35.65798604534883 37.0 36.0 37.0 35.0 37.0 8 35.665724740338945 37.0 37.0 37.0 35.0 37.0 9 37.28998325744892 39.0 38.0 39.0 35.0 39.0 10-11 37.24028780521064 39.0 38.0 39.0 35.0 39.0 12-13 37.16735732846461 39.0 37.5 39.0 34.5 39.0 14-15 38.32184713520557 40.0 38.0 41.0 34.0 41.0 16-17 38.283242676080874 40.0 38.0 41.0 34.0 41.0 18-19 38.3080288487034 40.0 38.0 41.0 34.0 41.0 20-21 38.266030424472916 40.0 38.0 41.0 34.0 41.0 22-23 38.136288911279635 40.0 38.0 41.0 34.0 41.0 24-25 38.08631125993379 40.0 38.0 41.0 34.0 41.0 26-27 37.88852377669528 40.0 37.5 41.0 34.0 41.0 28-29 37.69707725058523 40.0 37.0 41.0 34.0 41.0 30-31 37.36151599633331 40.0 36.0 41.0 33.0 41.0 32-33 36.93052409487951 39.5 35.0 41.0 33.0 41.0 34-35 36.55649853407981 39.0 35.0 41.0 32.5 41.0 36-37 36.19253554951174 39.0 35.0 41.0 31.0 41.0 38-39 35.86860885309737 38.5 35.0 41.0 30.5 41.0 40-41 35.629588102940176 39.0 35.0 41.0 30.0 41.0 42-43 35.31289820377427 38.0 35.0 41.0 29.0 41.0 44-45 34.86987401419708 38.0 35.0 41.0 25.0 41.0 46-47 34.27759111811453 38.0 35.0 41.0 21.0 41.0 48-49 33.896071182357446 38.5 35.0 41.0 7.0 41.0 50-51 32.818474003590936 38.0 34.0 40.0 2.0 40.5 52-53 32.93098811354631 38.0 34.0 39.5 2.0 40.5 54-55 33.330824475943004 38.0 35.0 40.5 2.0 41.0 56-57 33.18305631103266 38.0 35.0 41.0 2.0 41.0 58-59 32.987448768551275 38.0 35.0 41.0 2.0 41.0 60-61 32.65905044735187 38.0 34.0 40.5 2.0 41.0 62-63 32.27647368540671 37.5 33.5 40.0 2.0 41.0 64-65 31.85139849544315 37.0 33.0 40.0 2.0 41.0 66-67 31.414192531761607 36.5 33.0 40.0 2.0 41.0 68-69 30.966662626232015 36.0 32.0 39.0 2.0 41.0 70-71 30.48008128849461 35.0 31.5 39.0 2.0 41.0 72-73 29.948992037818467 35.0 31.0 38.0 2.0 40.0 74-75 29.43694081015765 35.0 30.0 37.0 2.0 40.0 76-77 28.906931113114492 35.0 29.0 37.0 2.0 39.0 78-79 28.390444245789737 35.0 28.5 36.0 2.0 39.0 80-81 27.889709391737817 35.0 27.5 36.0 2.0 38.5 82-83 27.375358904234126 35.0 26.0 35.5 2.0 37.0 84-85 26.904484124879733 34.0 25.0 35.0 2.0 37.0 86-87 26.461647436723005 34.0 22.5 35.0 2.0 36.0 88-89 25.96872324790339 34.0 19.0 35.0 2.0 36.0 90-91 25.512022818354687 34.0 6.0 35.0 2.0 36.0 92-93 25.078968401275766 34.0 2.0 35.0 2.0 35.0 94-95 24.60163145175342 34.0 2.0 35.0 2.0 35.0 96-97 24.03460442882143 33.0 2.0 35.0 2.0 35.0 98-99 23.454759126963083 33.0 2.0 35.0 2.0 35.0 100-101 21.467437253312525 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19916978737273183 1101 2 0.18293919399413028 1101 3 -0.1460282458472335 1101 4 0.2814977534285248 1101 5 -0.058822583082779545 1101 6 0.20932926291025922 1101 7 -0.10633322753599117 1101 8 -0.002538352233166563 1101 9 0.12404222044553848 1101 10-11 0.25175284698621425 1101 12-13 0.21337855775242787 1101 14-15 0.2108839106406606 1101 16-17 0.15219495700796415 1101 18-19 0.2840887695868446 1101 20-21 0.3192130310737795 1101 22-23 0.07827986064186376 1101 24-25 0.008468543619670754 1101 26-27 0.10065014658727023 1101 28-29 -0.017399780645497742 1101 30-31 0.2826617285400417 1101 32-33 0.3461210785808859 1101 34-35 0.508956763859473 1101 36-37 0.15059411870448258 1101 38-39 -0.12258556173025426 1101 40-41 -0.2142469527053663 1101 42-43 -0.4313333197554243 1101 44-45 0.03672176814647088 1101 46-47 -0.3054872470818424 1101 48-49 0.35486694077228975 1101 50-51 -0.15222742554389868 1101 52-53 0.2545763453133105 1101 54-55 0.10429506773146358 1101 56-57 -0.3698569752682417 1101 58-59 -0.46304397630606076 1101 60-61 -0.7179496845322362 1101 62-63 -0.525428704479701 1101 64-65 -0.46962862621683144 1101 66-67 -0.17782565785375937 1101 68-69 -0.5713890440534044 1101 70-71 -0.4297780778385807 1101 72-73 -0.6786811393810481 1101 74-75 -0.8962014418643172 1101 76-77 -0.42016160416606496 1101 78-79 -0.3186315294739117 1101 80-81 -0.384841090264743 1101 82-83 -0.7002520862746415 1101 84-85 -0.6407015380824781 1101 86-87 -0.47005670114914366 1101 88-89 -0.680430893632014 1101 90-91 -0.5938991471668871 1101 92-93 -0.7486230341635576 1101 94-95 -0.7200804922764981 1101 96-97 -0.720113982216045 1101 98-99 -1.1070875573703347 1101 100-101 -1.290768043751239 1102 1 0.10541978737273183 1102 2 -0.10723937743444267 1102 3 -0.08799253156152176 1102 4 0.3395334677142401 1102 5 0.09742741691722046 1102 6 0.20932926291025922 1102 7 0.29098820103543943 1102 8 0.12692593348111814 1102 9 0.351720791874115 1102 10-11 0.22273498984335305 1102 12-13 0.13971784346671257 1102 14-15 -0.24447323221648531 1102 16-17 -0.015215757277758257 1102 18-19 -0.041804087556009506 1102 20-21 0.13394517393091832 1102 22-23 0.19435128921329436 1102 24-25 0.30311140076251775 1102 26-27 0.37966800373013143 1102 28-29 0.13885021935450226 1102 30-31 0.3451617285400417 1102 32-33 -0.02887892141911408 1102 34-35 0.07368890671661887 1102 36-37 0.032290547275913184 1102 38-39 -0.12704984744453895 1102 40-41 -0.2745148098482204 1102 42-43 -0.28177974832685493 1102 44-45 -0.2958675175678209 1102 46-47 -0.280933675653273 1102 48-49 -0.33486520208484905 1102 50-51 -0.19687028268675277 1102 52-53 -0.15390579754383538 1102 54-55 -0.5608835036971058 1102 56-57 -0.5394998324111029 1102 58-59 -0.5746511191632067 1102 60-61 -0.5393782559608056 1102 62-63 -0.471857275908274 1102 64-65 -0.3245393405025503 1102 66-67 -0.20684351499662057 1102 68-69 -0.1495140440534044 1102 70-71 -0.2958495064100113 1102 72-73 -0.029127567952475175 1102 74-75 -0.16405858472146306 1102 76-77 -0.11212588988034966 1102 78-79 -0.08648867233105406 1102 80-81 -0.14823394740760065 1102 82-83 -0.11096637198892623 1102 84-85 0.22760203334609486 1102 86-87 0.36030044170799513 1102 88-89 0.38206910636798597 1102 90-91 -0.049256290024029425 1102 92-93 -0.14371231987784228 1102 94-95 -0.48347334941935216 1102 96-97 -0.641988982216045 1102 98-99 -0.22762327165605 1102 100-101 -0.5988037580369507 1103 1 -0.0023496361861674586 1103 2 0.22241287820465772 1103 3 0.11446674162143466 1103 4 0.4079075278646158 1103 5 0.33975197832072723 1103 6 0.3286901651659022 1103 7 0.1598791784790521 1103 8 0.11682255002247643 1103 9 0.3135785612976676 1103 10-11 0.3827835487405977 1103 12-13 0.30000136476997596 1103 14-15 0.42419061490130616 1103 16-17 0.38496438056685633 1103 18-19 0.4632084437723094 1103 20-21 0.4568618405975897 1103 22-23 0.24874508620577984 1103 24-25 0.521117353143481 1103 26-27 0.4462015250333877 1103 28-29 0.5418107456702899 1103 30-31 0.46491423480571115 1103 32-33 0.2786868680545709 1103 34-35 0.015339909222880976 1103 36-37 -0.08734447778675047 1103 38-39 0.014632483382520434 1103 40-41 0.23147673150516113 1103 42-43 0.059385665206974636 1103 44-45 -0.19886720428460336 1103 46-47 -0.8353274726457585 1103 48-49 -0.7227489740146709 1103 50-51 -0.6842606335639445 1103 52-53 -0.5495041684711524 1103 54-55 -0.6337218495617662 1103 56-57 -1.0049995191278924 1103 58-59 -1.0817391517446566 1103 60-61 -1.2827993085923843 1103 62-63 -1.710030834805515 1103 64-65 -1.408930004662949 1103 66-67 -1.4591931390567687 1103 68-69 -1.627740861096008 1103 70-71 -1.6557727520240455 1103 72-73 -1.7548481193309229 1103 74-75 -1.73795426141319 1103 76-77 -1.4805469425119284 1103 78-79 -1.446176955539073 1103 80-81 -1.4804707895128644 1103 82-83 -1.7981139283799052 1103 84-85 -1.6561307360774613 1103 86-87 -1.7064680420012728 1103 88-89 -1.4303525728300102 1103 90-91 -1.7099314153373122 1103 92-93 -1.986992395065812 1103 94-95 -2.009084251674995 1103 96-97 -1.9683909245719349 1103 98-99 -1.60090021401194 1103 100-101 -1.3560484322224156 1104 1 0.6793008419404813 1104 2 0.8041838291872665 1104 3 0.8363062115408724 1104 4 0.7057390164573434 1104 5 0.7790261293635723 1104 6 0.7135444683057273 1104 7 0.6096194088833897 1104 8 0.7302176256944861 1104 9 0.8922059236950801 1104 10-11 1.0788719456986584 1104 12-13 1.0248055963790321 1104 14-15 1.1708681994205463 1104 16-17 1.1807817135990106 1104 18-19 1.0373651337805967 1104 20-21 0.9978131996819926 1104 22-23 1.1381453572696998 1104 24-25 1.227841244416723 1104 26-27 1.30244352488279 1104 28-29 1.3527718931742427 1104 30-31 1.247771323880329 1104 32-33 1.3070921086238059 1104 34-35 1.5144269508613135 1104 36-37 1.4663130028246556 1104 38-39 1.9193365719300743 1104 40-41 2.1002648498697454 1104 42-43 1.9715157758914188 1104 44-45 1.5566481936522933 1104 46-47 1.5354653893375314 1104 48-49 1.7875194545675086 1104 50-51 1.6082531078711853 1104 52-53 1.6306780467234958 1104 54-55 1.6000990223605243 1104 56-57 1.5445072951180236 1104 58-59 1.4827044142518773 1104 60-61 1.6274672376014294 1104 62-63 1.6056833433437205 1104 64-65 1.5357936607236944 1104 66-67 1.6071892869653652 1104 68-69 1.4901625347326188 1104 70-71 1.466422489298143 1104 72-73 1.0570542223602146 1104 74-75 1.1147676721761464 1104 76-77 0.8562217496046287 1104 78-79 0.8388542921079392 1104 80-81 0.7538583272705104 1104 82-83 0.45928385793136783 1104 84-85 0.48251657963671235 1104 86-87 0.2991320634124719 1104 88-89 0.12124369251145595 1104 90-91 0.3856092066038066 1104 92-93 0.4980143815323359 1104 94-95 0.5411472820950571 1104 96-97 0.7412977283909434 1104 98-99 0.8587884389509384 1104 100-101 1.108957967898057 1105 1 -0.4440763596337902 1105 2 -0.6064798931428008 1105 3 -0.7200131302574313 1105 4 -0.6002820331749135 1105 5 -0.41994810175497577 1105 6 -0.46324038735648543 1105 7 -0.32123023405643636 1105 8 -0.5952324838277079 1105 9 -0.8161622845929912 1105 10-11 -0.6135220492793465 1105 12-13 -0.6037894920401001 1105 14-15 -0.8453642369586305 1105 16-17 -0.5747611781431985 1105 18-19 -0.6445752790201453 1105 20-21 -0.4717342866499976 1105 22-23 -0.6886047718418311 1105 24-25 -0.9588273218219427 1105 26-27 -0.7081080039758589 1105 28-29 -1.0910512329276614 1105 30-31 -0.8190906425328635 1105 32-33 -0.7839359753610253 1105 34-35 -0.8595690956544573 1105 36-37 -0.8906403211295455 1105 38-39 -0.6605968243265821 1105 40-41 -0.33237454606636163 1105 42-43 -0.2772598905912318 1105 44-45 -0.2072023575203943 1105 46-47 0.4241178210864973 1105 48-49 -0.31768418548733734 1105 50-51 -0.5045725648444304 1105 52-53 -0.1687805752557452 1105 54-55 -0.0804078071944403 1105 56-57 0.04475661394337749 1105 58-59 -0.3189179389616683 1105 60-61 -0.17682638874088497 1105 62-63 -0.214762581183912 1105 64-65 -0.20015989474083895 1105 66-67 -0.0300874391222834 1105 68-69 0.11593497787902152 1105 70-71 0.19371221558169083 1105 72-73 0.3532449868785825 1105 74-75 0.3681824496590984 1105 76-77 0.6664010810740102 1105 78-79 0.5819223383387744 1105 80-81 0.2508843098538094 1105 82-83 0.46799220833116806 1105 84-85 0.02807069072605728 1105 86-87 0.6125881565864795 1105 88-89 0.8458176244474167 1105 90-91 0.9696474592350093 1105 92-93 0.7939914442004046 1105 94-95 0.6557716120507102 1105 96-97 0.3122289342036346 1105 98-99 0.334283960116327 1105 100-101 0.02818757272771677 1106 1 0.37165355360649244 1106 2 0.18500530969660645 1106 3 0.37780587457779546 1106 4 0.557102830169967 1106 5 0.5917455987354003 1106 6 0.48190895594449756 1106 7 0.47479870870958507 1106 8 0.48698348956140336 1106 9 0.23959712009134648 1106 10-11 0.3713292932672019 1106 12-13 0.40810996271110156 1106 14-15 0.7474652270515207 1106 16-17 0.5522835048237766 1106 18-19 0.7222159832822399 1106 20-21 0.7850298846747208 1106 22-23 0.794797717784725 1106 24-25 0.7986655625098678 1106 26-27 0.6317894618174904 1106 28-29 0.5342531119164917 1106 30-31 0.4344843377490193 1106 32-33 0.18625537610154907 1106 34-35 0.21370514048285116 1106 36-37 0.32259825093589 1106 38-39 0.20400829895451267 1106 40-41 -0.15407133287065733 1106 42-43 -0.43039249921233136 1106 44-45 -0.16664304885471637 1106 46-47 -0.5662974595965977 1106 48-49 -0.7748187144815475 1106 50-51 -0.8634508611991514 1106 52-53 -1.033591452797669 1106 54-55 -1.403784920462158 1106 56-57 -1.4645109008880794 1106 58-59 -1.3918921168019267 1106 60-61 -1.5507418923244423 1106 62-63 -1.4010559181750963 1106 64-65 -1.55009645384375 1106 66-67 -1.552548945929324 1106 68-69 -1.2979054253993283 1106 70-71 -1.2825488865753023 1106 72-73 -1.1875543684247312 1106 74-75 -1.4845832305301983 1106 76-77 -1.332167212194399 1106 78-79 -1.277770401965057 1106 80-81 -1.1342876664158652 1106 82-83 -1.3434597309027403 1106 84-85 -1.1926864849537857 1106 86-87 -0.9847593280676783 1106 88-89 -0.7668756988268193 1106 90-91 -0.6498871932117503 1106 92-93 -1.075346027079732 1106 94-95 -1.055898083775908 1106 96-97 -0.8605360601381236 1106 98-99 -0.410400721478954 1106 100-101 -0.7441882031373055 1107 1 -0.21743735548441379 1107 2 -0.17106080600586893 1107 3 -0.13181396013294844 1107 4 0.0028548962856689286 1107 5 0.1099274169172233 1107 6 -0.1250993085183083 1107 7 -0.2302260846788471 1107 8 0.07703307633826739 1107 9 -0.058386350983028024 1107 10-11 0.06650284698621789 1107 12-13 -0.00412144224757327 1107 14-15 0.03936605349780109 1107 16-17 0.04205209986510283 1107 18-19 0.046910198158272465 1107 20-21 0.08719517393091536 1107 22-23 0.08279771778472167 1107 24-25 0.25045068647681035 1107 26-27 0.07107871801584054 1107 28-29 0.0786002193545059 1107 30-31 0.0040545856829012905 1107 32-33 0.3743710785808858 1107 34-35 -0.019203950426238237 1107 36-37 0.26309411870447974 1107 38-39 0.17984300969830969 1107 40-41 0.06900304729462903 1107 42-43 0.3086488231017128 1107 44-45 0.8941503395750416 1107 46-47 1.0616198957752943 1107 48-49 1.1697597979151553 1107 50-51 1.2548797173132513 1107 52-53 1.4329870595990215 1107 54-55 1.5543307820171819 1107 56-57 1.536018024731753 1107 58-59 1.4973310236939383 1107 60-61 1.2876217440391926 1107 62-63 1.1262498669488679 1107 64-65 1.1531928023545923 1107 66-67 1.166227913574815 1107 68-69 1.308557384518025 1107 70-71 1.4045254935899898 1107 72-73 1.037801003476094 1107 74-75 1.0568878438499674 1107 76-77 1.3428741101196486 1107 78-79 0.7262791848118013 1107 80-81 0.6472660525923963 1107 82-83 0.7426229137253593 1107 84-85 0.7988341762032398 1107 86-87 0.7244968702794274 1107 88-89 0.6940333920822681 1107 90-91 1.1782615671188275 1107 92-93 1.0963233944078752 1107 94-95 0.7457052220092173 1107 96-97 0.7371003034982415 1107 98-99 0.35459101405823645 1107 100-101 0.497249813391619 1108 1 -0.049065262461159165 1108 2 0.1771252405057595 1108 3 -0.06719380509418826 1108 4 -0.23407533627246835 1108 5 -0.04743692416805345 1108 6 0.02646658295455495 1108 7 0.05033205485603531 1108 8 0.2041338515320632 1108 9 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-1.4784302377064371 2316 78-79 -1.5071990760577627 2316 80-81 -2.275226701030789 2316 82-83 -2.2830872312021775 2316 84-85 -2.3545861136518376 2316 86-87 -2.4918799413147745 2316 88-89 -2.283937622410484 2316 90-91 -1.8693326357797204 2316 92-93 -2.1682853012443033 2316 94-95 -1.9567005750922313 2316 96-97 -2.420088102298859 2316 98-99 -2.356220580144665 2316 100-101 -2.0751559837098306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2259.0 3 368.0 4 311.0 5 340.0 6 338.0 7 375.0 8 478.0 9 551.0 10 647.0 11 969.0 12 1503.0 13 2389.0 14 2421.0 15 2930.0 16 3699.0 17 5271.0 18 4737.0 19 2681.0 20 2787.0 21 2701.0 22 2583.0 23 2706.0 24 3065.0 25 3156.0 26 3489.0 27 3801.0 28 4109.0 29 4574.0 30 5430.0 31 6065.0 32 7140.0 33 8956.0 34 12061.0 35 23388.0 36 25622.0 37 41619.0 38 55407.0 39 13068.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90731395914397 23.282845938715955 16.014485043774318 16.795355058365757 2 13.906069281962798 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16.827699704983427 31.103771510044975 35.789529566120876 16.278999218850725 22-23 16.347256906323533 31.364540491752614 35.498948573985686 16.78925402793816 24-25 16.446778289175498 32.21805644844713 35.98090697555342 15.354258286823955 26-27 16.41368742841105 31.162718370009635 37.38251826229433 15.041075939284983 28-29 14.790752887589292 32.360721579692914 37.54709932624497 15.301426206472826 30-31 15.143821460399327 33.14567966779802 36.4237092095034 15.286789662299247 32-33 14.910261612143596 33.53500612412831 35.99868795765061 15.556044306077485 34-35 15.733645934068857 32.21149187687736 35.982649899209065 16.07221228984472 36-37 15.96326853698263 32.05535597400027 36.41807346292604 15.56330202609106 38-39 15.94428422502416 32.31982924583578 36.482913169529056 15.252973359611008 40-41 14.947583037901497 32.65998965354594 36.913447347199615 15.478979961352952 42-43 14.788362606922165 33.072813612979814 35.590050531186264 16.54877324891175 44-45 15.340283155465325 32.76322974559389 35.02816904088269 16.868318058058097 46-47 15.99888115720312 32.906156109014866 34.220796395347286 16.874166338434723 48-49 15.984821669844676 32.487640013244885 34.400916484930136 17.1266218319803 50-51 15.742455004024377 32.784888887197525 34.947778219645436 16.524877889132654 52-53 15.381980651692405 33.25062223609198 34.61554562684102 16.751851485374598 54-55 14.86717884907362 32.43492125100363 34.91287952297781 17.785020376944942 56-57 16.110703184742455 32.83773449953775 33.50732555440998 17.54423676130981 58-59 16.27422229068841 31.89173974481131 34.10890307660415 17.72513488789613 60-61 15.889570318886426 32.030272728309676 34.550718796695165 17.529438156108725 62-63 16.551131132197508 31.546717700145336 34.51969684287421 17.38245432478294 64-65 16.09558932669582 31.941701015037165 34.804509246467155 17.158200411799854 66-67 15.705977284491945 31.63404737979964 33.9628817636491 18.697093572059316 68-69 15.446956402574811 32.18036612244432 34.1824673866288 18.190210088352067 70-71 16.359518485035924 31.94347433030404 33.55778655374221 18.13922063091783 72-73 16.074035026956253 30.982280995887578 34.01093474359682 18.93274923355935 74-75 15.883670606863976 32.27567805206694 33.47886243693369 18.3617889041354 76-77 16.69593708793875 30.618826811071642 33.97772657303938 18.70750952795024 78-79 16.31558221425759 30.537213430050393 34.45402610447497 18.69317825121705 80-81 16.486731741281798 31.434425371870795 33.41481928192172 18.664023604925685 82-83 16.435460455841866 31.09991495061618 33.50336867861417 18.961255914927786 84-85 16.547368981840876 30.537163036363914 33.08065817312815 19.83480980866706 86-87 16.901491526326705 30.754858068112846 33.110210077078364 19.233440328482082 88-89 17.68603533743732 29.92435847422955 32.90986765111655 19.47973853721658 90-91 17.297352849935198 29.703692002595954 33.539385611486146 19.459569535982705 92-93 17.856809821321523 30.822179805088407 32.81868197743349 18.502328396156575 94-95 17.752829517188353 30.095176831740073 32.68061426263172 19.47137938843985 96-97 17.872259439707676 29.49356729598051 32.22194732034105 20.412225943970768 98-99 18.607156042216157 29.502974389422366 31.85317972315152 20.036689845209956 100-101 19.231032698045425 28.4884384704378 32.61736588930159 19.663162942215187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5233.0 1 3140.5 2 1220.0 3 1346.5 4 1092.0 5 1014.5 6 1726.5 7 2972.0 8 4150.5 9 4236.5 10 3082.0 11 2128.0 12 1909.0 13 1804.5 14 1687.0 15 1568.5 16 1425.5 17 1396.5 18 1395.5 19 1384.0 20 1491.5 21 1682.0 22 1836.5 23 1942.0 24 2099.5 25 2449.0 26 3001.5 27 3638.0 28 3844.0 29 3996.5 30 4561.5 31 5072.5 32 6117.5 33 7479.5 34 8643.5 35 9381.0 36 10312.5 37 11352.5 38 10975.5 39 10048.5 40 9189.0 41 8450.5 42 7563.5 43 6957.5 44 6934.0 45 6623.0 46 6604.5 47 7979.0 48 14719.5 49 14310.0 50 6710.0 51 3992.5 52 2419.0 53 1695.0 54 1319.0 55 1097.0 56 893.0 57 743.0 58 653.5 59 569.0 60 479.0 61 430.5 62 391.5 63 312.5 64 247.5 65 230.0 66 208.0 67 169.5 68 149.0 69 128.0 70 103.5 71 95.0 72 78.0 73 70.0 74 64.0 75 51.0 76 37.0 77 29.5 78 22.5 79 14.5 80 12.5 81 8.5 82 5.0 83 3.0 84 3.5 85 4.0 86 2.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3143963211842514 2 0.1109856892855249 3 0.10909173554345108 4 0.11553117826650203 5 0.1340919249388253 6 0.08636429063856545 7 0.09621285009734921 8 0.10151592057515586 9 0.088637035129054 10-11 0.08693247676118758 12-13 0.0907203842453352 14-15 0.09223554723899423 16-17 0.08087182478655142 18-19 0.09678103621997136 20-21 0.10757657254979204 22-23 0.11685694588595369 24-25 0.12935704058364078 26-27 0.12878885446101865 28-29 0.12367517935741937 30-31 0.1147735967696725 32-33 0.10871294479503632 34-35 0.314206925810044 36-37 0.4643974575564967 38-39 0.44091243115478146 40-41 0.41894256774672534 42-43 0.4507609906135653 44-45 0.4581474102076531 46-47 0.464586852930704 48-49 0.47538238926052473 50-51 0.4638292714338745 52-53 0.4681853650406443 54-55 0.45492768884612766 56-57 0.4359881514253896 58-59 0.41458647413995564 60-61 0.37462405018219835 62-63 0.44091243115478146 64-65 0.4731096447700362 66-67 0.48106425048674606 68-69 0.49204918219077415 70-71 0.4869355070871749 72-73 0.4763293661315616 74-75 0.4842839718482716 76-77 0.4615565269433859 78-79 0.4429957802710626 80-81 0.44375336176789215 82-83 0.4581474102076531 84-85 0.4583368055818605 86-87 0.4596625732013121 88-89 0.47178387715058445 90-91 0.48542034409351587 92-93 0.47860211062205016 94-95 0.48352639035144207 96-97 0.4840945764740642 98-99 0.47538238926052473 100-101 0.4659126205501557 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 263998.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.4976098303775 #Duplication Level Percentage of deduplicated Percentage of total 1 75.32528850482579 25.985424132001 2 10.80891153250689 7.457632254789809 3 4.363532550810888 4.515943302600777 4 2.219098964566886 3.0621444101849256 5 1.3999758435540721 2.4147910211440995 6 0.951983573616769 1.9704694732535852 7 0.7213993170313924 1.7420586519594845 8 0.5182655671823702 1.4303138660141366 9 0.3645427294587858 1.1318267562633049 >10 2.9163418356702864 19.629315373601315 >50 0.26352486466900177 6.170122500928037 >100 0.11968420937050499 7.9224085030947204 >500 0.014274263502904262 3.3988893855256475 >1k 0.010980202694541741 8.172031606300049 >5k 0.0021960405389083483 4.996628762339109 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6621 2.507973545254131 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6570 2.4886552170849776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4634 1.755316328154001 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3569 1.351904181092281 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2538 0.9613709194766626 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2016 0.7636421488041576 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1984 0.7515208448548852 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1585 0.6003833362373957 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1548 0.5863680785460496 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1300 0.4924279729391889 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1244 0.47121569102796235 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1156 0.4378821051674634 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 902 0.34166925507011414 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 895 0.3390177198312108 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 868 0.3287903696240123 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 826 0.31288115819059237 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 754 0.28560822430472954 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 684 0.25909287191569635 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 667 0.2526534291926454 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 661 0.2503806847021568 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 640 0.24242607898544685 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 544 0.20606216713762981 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 520 0.19697118917567555 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 509 0.1928044909431132 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 503 0.19053174645262463 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 489 0.18522867597481799 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 485 0.18371351298115896 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 482 0.1825771407359147 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 476 0.1803043962454261 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 450 0.17045583678664233 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 412 0.15606178834688142 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 398 0.15075871786907477 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 382 0.1446980658944386 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 382 0.1446980658944386 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 374 0.1416677399071205 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 353 0.13371313419041053 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 351 0.132955552693581 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 351 0.132955552693581 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 341 0.1291676452094334 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 326 0.12348578398321199 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 315 0.11931908575064962 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 307 0.11628875976333154 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 301 0.11401601527284298 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 289 0.10947052629186585 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 279 0.10568261880771823 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 273 0.10340987431722967 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 264 0.10000075758149682 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8939537420738036E-4 0.0 20-21 0.0 0.0 0.0 0.002651535238903325 0.0 22-23 0.0 0.0 0.0 0.008333396465124735 0.0 24-25 0.0 0.0 0.0 0.016477397556042092 0.0 26-27 0.0 0.0 0.0 0.0327653997378768 0.0 28-29 0.0 0.0 0.0 0.07045507920514549 0.0 30-31 0.0 0.0 0.0 0.1494329502496231 0.0 32-33 0.0 0.0 0.0 0.26060803490935536 0.0 34-35 0.0 0.0 0.0 0.4111773574042228 0.0 36-37 0.0 0.0 0.0 0.6202698505291706 0.0 38-39 0.0 0.0 0.0 0.8664838369987651 0.0 40-41 0.0 0.0 0.0 1.121410010681899 0.0 42-43 0.0 0.0 0.0 1.3553132978280138 0.0 44-45 0.0 0.0 0.0 1.6091030992659034 0.0 46-47 0.0 0.0 0.0 1.9346737475283904 0.0 48-49 0.0 0.0 0.0 2.253994348442034 0.0 50-51 0.0 0.0 0.0 2.57236797248464 0.0 52-53 0.0 0.0 0.0 2.8538094985568074 0.0 54-55 0.0 0.0 0.0 3.184304426548686 0.0 56-57 0.0 0.0 0.0 3.545292009787953 0.0 58-59 0.0 0.0 0.0 3.925219130447958 0.0 60-61 0.0 0.0 0.0 4.378821051674635 0.0 62-63 0.0 0.0 0.0 4.814809203100023 0.0 64-65 0.0 0.0 0.0 5.271820241062432 0.0 66-67 0.0 0.0 0.0 5.765195190872658 0.0 68-69 0.0 0.0 0.0 6.342093500708339 0.0 70-71 0.0 0.0 0.0 6.97675739967727 0.0 72-73 0.0 0.0 0.0 7.588883249115524 0.0 74-75 0.0 0.0 0.0 8.268812642520018 0.0 76-77 0.0 0.0 0.0 8.928666126258532 0.0 78-79 0.0 0.0 0.0 9.678293017371344 0.0 80-81 0.0 0.0 0.0 10.485117311494784 0.0 82-83 0.0 0.0 0.0 11.270161137584376 0.0 84-85 0.0 0.0 0.0 12.161266373230099 0.0 86-87 0.0 0.0 0.0 13.080591519632724 0.0 88-89 0.0 0.0 0.0 13.938931355540571 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACTTG 20 5.3555344E-4 47.553776 64-65 TAGGACG 50 2.865866E-5 47.373413 4 TTAGGCA 40 5.451838E-4 47.37341 4 AGGCTTA 65 2.636465E-6 43.712723 6 GGACGTG 55 5.0427516E-5 43.05041 6 ATACCAA 55 5.0427516E-5 43.05041 6 GGCATTC 45 9.756815E-4 42.093735 7 GGTACTC 70 4.3770724E-6 40.60578 3 ACGACTT 25 0.0016060643 38.043022 62-63 ACTCCCG 75 7.0479055E-6 37.88436 6 CCTTTAG 55 0.0025809598 34.53198 1 CATTCCG 55 0.0026172597 34.4338 9 GACGACT 30 0.0039272024 31.70252 62-63 GTGGTAC 90 2.4123718E-5 31.654314 1 CCCGCTG 90 2.4597997E-5 31.564318 9 CTGTAGG 80 4.4945898E-4 29.675915 1 GTAGGAC 80 4.554986E-4 29.608381 3 TGACGAC 35 0.008341329 27.173588 60-61 AATGACG 35 0.008341329 27.173588 74-75 TGTAAAC 35 0.008341329 27.173588 58-59 >>END_MODULE