##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939089_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 263998 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37489299161357 33.0 31.0 34.0 31.0 34.0 2 32.54259501965924 34.0 31.0 34.0 31.0 34.0 3 32.590754475412695 34.0 31.0 34.0 31.0 34.0 4 36.10084924885795 37.0 35.0 37.0 35.0 37.0 5 36.088190062045925 37.0 35.0 37.0 35.0 37.0 6 36.17823998666657 37.0 36.0 37.0 35.0 37.0 7 36.12953886014288 37.0 36.0 37.0 35.0 37.0 8 36.14908446276108 37.0 37.0 37.0 35.0 37.0 9 37.80615762240623 39.0 38.0 39.0 35.0 39.0 10-11 37.7746952628429 39.0 38.0 39.0 35.0 39.0 12-13 37.72385586254441 39.0 38.0 39.0 35.0 39.0 14-15 38.90684020333487 40.0 38.0 41.0 35.0 41.0 16-17 38.85665042916992 40.0 38.0 41.0 35.5 41.0 18-19 38.94892006757627 40.0 38.5 41.0 35.0 41.0 20-21 39.02228425972925 41.0 38.5 41.0 35.0 41.0 22-23 38.93471541451072 40.5 38.0 41.0 35.0 41.0 24-25 38.82116152395094 40.0 38.0 41.0 35.0 41.0 26-27 38.549850756445124 40.0 38.0 41.0 34.5 41.0 28-29 38.32028083546088 40.0 37.0 41.0 34.0 41.0 30-31 37.94155637542708 40.0 36.5 41.0 34.0 41.0 32-33 37.483441162433046 40.0 35.0 41.0 33.0 41.0 34-35 37.23591087811271 40.0 35.0 41.0 33.0 41.0 36-37 36.990916597853015 40.0 35.0 41.0 33.0 41.0 38-39 36.71346184440791 39.0 35.0 41.0 33.0 41.0 40-41 36.45019280449094 39.0 35.0 41.0 31.5 41.0 42-43 36.13790634777536 39.0 35.0 41.0 31.0 41.0 44-45 35.715355798149986 39.0 35.0 41.0 30.0 41.0 46-47 35.15226819900151 39.0 35.0 41.0 25.5 41.0 48-49 34.60426215350116 39.0 35.0 41.0 22.0 41.0 50-51 34.267839150296595 39.0 35.0 41.0 19.5 41.0 52-53 34.000625004734886 38.0 35.0 41.0 14.0 41.0 54-55 33.749954545110185 38.0 35.0 40.0 5.0 41.0 56-57 33.43859233782074 38.0 34.0 40.0 2.0 41.0 58-59 33.114370184622615 38.0 34.0 40.0 2.0 41.0 60-61 32.67009030371442 37.5 33.0 40.0 2.0 41.0 62-63 32.226297168917945 37.0 33.0 40.0 2.0 41.0 64-65 31.738846506412926 36.0 32.5 39.5 2.0 41.0 66-67 31.28326350957204 36.0 32.0 39.0 2.0 41.0 68-69 30.762142137440435 35.5 31.0 39.0 2.0 40.0 70-71 30.303284494579504 35.0 31.0 38.5 2.0 40.0 72-73 29.8167618694081 35.0 30.5 37.5 2.0 40.0 74-75 29.2988999916666 35.0 29.5 37.0 2.0 39.0 76-77 27.340068864158063 32.5 26.5 35.0 2.0 37.5 78-79 28.14032303275025 34.0 28.0 36.0 2.0 38.0 80-81 28.069934241926077 34.0 29.0 36.0 2.0 37.5 82-83 27.71316638762415 34.0 27.5 35.0 2.0 37.0 84-85 27.29547572330093 34.0 26.5 35.0 2.0 36.5 86-87 26.745267009598557 34.0 25.5 35.0 2.0 36.0 88-89 26.17099182569565 34.0 23.0 35.0 2.0 36.0 90-91 25.672311911453875 33.5 19.5 35.0 2.0 35.0 92-93 25.396798460594397 33.0 17.5 35.0 2.0 35.0 94-95 25.083559345146554 33.0 6.5 35.0 2.0 35.0 96-97 24.555000416669824 33.0 2.0 35.0 2.0 35.0 98-99 23.926641489708253 33.0 2.0 35.0 2.0 35.0 100-101 20.930630156289062 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28585905334108475 1101 2 0.1798518732066441 1101 3 0.32958304233432756 1101 4 0.0913081270786904 1101 5 0.009804321560892504 1101 6 0.11662944656222862 1101 7 -0.0017827771585245955 1101 8 0.0627835914249033 1101 9 0.5594584925383685 1101 10-11 0.1746729250385073 1101 12-13 0.2697100414926794 1101 14-15 0.3130371577795259 1101 16-17 0.33696700257558376 1101 18-19 0.18646639896732609 1101 20-21 0.2428677398056891 1101 22-23 0.17680429159560873 1101 24-25 0.20170204608140807 1101 26-27 0.1600511870398762 1101 28-29 0.17888979078858824 1101 30-31 0.5456770612104265 1101 32-33 0.5787066423962415 1101 34-35 0.3963850874650703 1101 36-37 0.6932928533903322 1101 38-39 0.9882959929701158 1101 40-41 0.599571905147279 1101 42-43 0.4218413377034267 1101 44-45 0.6662932481961761 1101 46-47 0.7540493385094038 1101 48-49 0.6932619656519492 1101 50-51 0.7493405062550167 1101 52-53 0.5618741726917236 1101 54-55 0.3720774173311838 1101 56-57 -7.44114627956094E-4 1101 58-59 0.2563221760725085 1101 60-61 0.6418611193599162 1101 62-63 0.32783287379313464 1101 64-65 0.33397663280132406 1101 66-67 0.5291413892556456 1101 68-69 1.0557310967429707 1101 70-71 0.9024853657833383 1101 72-73 0.6865192740891075 1101 74-75 1.0781577956722188 1101 76-77 1.168430584842259 1101 78-79 1.27564249635574 1101 80-81 1.1799376996798294 1101 82-83 0.7991383638491314 1101 84-85 1.0921787339068771 1101 86-87 0.9050828911001325 1101 88-89 1.0485592600984184 1101 90-91 0.984890289902161 1101 92-93 0.7868738879918773 1101 94-95 0.7760222383425024 1101 96-97 0.47888960326701024 1101 98-99 0.6270662531565883 1101 100-101 0.9772300074565727 1102 1 1.447676268000464E-4 1102 2 -0.007648126793355914 1102 3 -0.05880981480852654 1102 4 -0.08726330149273309 1102 5 0.08123289298946901 1102 6 0.07198658941937452 1102 7 0.0026815085557601037 1102 8 2.835914249033067E-4 1102 9 0.09963706396693794 1102 10-11 0.09208363932422259 1102 12-13 0.05096004149267941 1102 14-15 0.0764300149223871 1102 16-17 0.05125271686129196 1102 18-19 -0.2242478867469586 1102 20-21 -0.1209715459085956 1102 22-23 0.08975072016703223 1102 24-25 -0.03267295391859193 1102 26-27 0.1265690441827374 1102 28-29 0.007014790788588243 1102 30-31 0.3537127754961418 1102 32-33 0.2684387852533874 1102 34-35 0.3160279446079315 1102 36-37 0.2803464248189016 1102 38-39 0.15793885011297704 1102 40-41 -0.009803094852721017 1102 42-43 -0.1428908051537121 1102 44-45 0.2533468196247526 1102 46-47 0.6446743385093967 1102 48-49 0.8562083942233727 1102 50-51 0.5975547919693014 1102 52-53 0.6399991726917236 1102 54-55 0.16002384590261443 1102 56-57 0.7358630282291898 1102 58-59 0.7518578903582167 1102 60-61 0.4320396907884856 1102 62-63 0.47515430236456524 1102 64-65 0.47683377565846996 1102 66-67 0.7233378178270762 1102 68-69 0.16287395388582482 1102 70-71 0.2596282229261959 1102 72-73 0.2490192740891075 1102 74-75 0.4710149385293647 1102 76-77 0.5411984419851201 1102 78-79 0.5859103534985941 1102 80-81 0.9143126996798294 1102 82-83 1.1227990781348467 1102 84-85 0.9560180196211654 1102 86-87 1.1216007482429866 1102 88-89 1.3789164029555643 1102 90-91 1.408997432759307 1102 92-93 0.9252667451347314 1102 94-95 0.2804865240567871 1102 96-97 0.2043360318384373 1102 98-99 0.4909055388708765 1102 100-101 0.6066942931708574 1103 1 0.2205395044689027 1103 2 0.16199473034949818 1103 3 0.10644707742204673 1103 4 0.10477930502355548 1103 5 0.15219153960600806 1103 6 0.09775413327902527 1103 7 0.1271332629417259 1103 8 0.24268647363041396 1103 9 0.29363769053335176 1103 10-11 0.3633477370685867 1103 12-13 0.2317636129212488 1103 14-15 0.36100864900759433 1103 16-17 0.37001838102170126 1103 18-19 0.12396639896732609 1103 20-21 0.3863514490788731 1103 22-23 0.24854614623219362 1103 24-25 0.2705068706428122 1103 26-27 0.3175543198719524 1103 28-29 0.28274317424723705 1103 30-31 0.09059404116030123 1103 32-33 -0.04018433504736407 1103 34-35 0.2061438593948921 1103 36-37 0.2604921015106285 1103 38-39 -0.01722562357123536 1103 40-41 0.40811670464602656 1103 42-43 0.09821978381872043 1103 44-45 -0.047052616465478536 1103 46-47 0.09744689490037217 1103 48-49 0.31054736665445404 1103 50-51 0.6547681378339618 1103 52-53 0.10698695464660801 1103 54-55 0.13590103888507343 1103 56-57 0.2266211735925978 1103 58-59 0.3229340181777687 1103 60-61 0.39605128226718733 1103 62-63 0.4351715329410055 1103 64-65 0.2896862192674874 1103 66-67 0.3092165772255733 1103 68-69 0.2132342295750469 1103 70-71 0.16587822292619592 1103 72-73 0.3036088730865991 1103 74-75 -0.054595963726278285 1103 76-77 -0.006263964029919578 1103 78-79 0.07439719560385427 1103 80-81 -0.04464720006954437 1103 82-83 -0.08619998201552903 1103 84-85 -0.3412485843888611 1103 86-87 -0.3113174848397193 1103 88-89 -0.24408641659330854 1103 90-91 -0.2450595847845527 1103 92-93 -0.09043657566727248 1103 94-95 0.08977537117458212 1103 96-97 -0.20367618620667471 1103 98-99 -0.4283457142619582 1103 100-101 -0.2540121604632226 1104 1 0.17208682771263994 1104 2 0.18881876468426384 1104 3 0.1934510680854018 1104 4 0.18105368195300287 1104 5 0.27072553554005196 1104 6 0.32292543062109047 1104 7 0.33141004932829077 1104 8 0.19493028057266315 1104 9 0.2778444520723937 1104 10-11 0.3508110304952865 1104 12-13 0.19957477171953286 1104 14-15 0.20835636378810563 1104 16-17 0.3813032993260421 1104 18-19 0.5054041672076721 1104 20-21 0.3871044044286265 1104 22-23 0.5289655423620019 1104 24-25 0.441173612605013 1104 26-27 0.5937383421594333 1104 28-29 0.5556141929958258 1104 30-31 0.508841914061442 1104 32-33 0.5921569796126747 1104 34-35 0.3514548299543421 1104 36-37 0.5917830434577667 1104 38-39 0.7615466827310016 1104 40-41 0.668385133228206 1104 42-43 1.0558848692791543 1104 44-45 0.6434544989625763 1104 46-47 0.3572164905633528 1104 48-49 0.2706972047690499 1104 50-51 0.0014193689159611722 1104 52-53 0.14081347373402764 1104 54-55 0.32955174289831746 1104 56-57 0.6157296744584997 1104 58-59 0.4598495365875337 1104 60-61 0.5668592033574598 1104 62-63 0.30557850530754394 1104 64-65 0.027770700857733033 1104 66-67 0.6699292494641078 1104 68-69 0.748538423536349 1104 70-71 0.854719424642937 1104 72-73 0.5097680478475368 1104 74-75 0.9366226945072924 1104 76-77 0.6609485952653138 1104 78-79 0.8990809543569647 1104 80-81 0.8635769547380754 1104 82-83 0.679809731108481 1104 84-85 0.4157915481312777 1104 86-87 0.6866777715415786 1104 88-89 1.0131419547642686 1104 90-91 1.2355800507850603 1104 92-93 1.103138832811002 1104 94-95 1.2763671187839485 1104 96-97 1.1767168564858927 1104 98-99 1.0737343248917206 1104 100-101 0.691812702122423 1105 1 0.05227132364339582 1105 2 0.030251991760287922 1105 3 -0.025059073848247237 1105 4 -0.03710029023013561 1105 5 -0.03283794281373531 1105 6 0.11023866410817362 1105 7 0.14933607287111528 1105 8 0.020567379213879633 1105 9 -0.008302325481786 1105 10-11 -0.15073830791940424 1105 12-13 -0.010974976290370364 1105 14-15 0.05156153537289043 1105 16-17 0.05855117566390078 1105 18-19 0.02593735332417424 1105 20-21 0.12890804804516875 1105 22-23 0.12397382330870244 1105 24-25 -0.005053659312778791 1105 26-27 0.41470071282528664 1105 28-29 0.15454924544182802 1105 30-31 -0.22029989492472168 1105 32-33 0.13231511898190007 1105 34-35 0.17556039866838802 1105 36-37 0.11649231989892428 1105 38-39 0.1030244310258368 1105 40-41 -0.09242016657767493 1105 42-43 0.017943886606808235 1105 44-45 0.2322387135192372 1105 46-47 0.04918308184075215 1105 48-49 0.30954642030518187 1105 50-51 0.6460599045952691 1105 52-53 0.9164977648671808 1105 54-55 0.8923889911308294 1105 56-57 0.9072100940264605 1105 58-59 1.2098361802218918 1105 60-61 1.2204306955306308 1105 62-63 1.5177132087071854 1105 64-65 1.661601484609271 1105 66-67 1.0292154852841016 1105 68-69 0.9447167221134514 1105 70-71 1.153430090146113 1105 72-73 1.56913264101264 1105 74-75 1.324990190455857 1105 76-77 1.6211110086715443 1105 78-79 1.4768595236230766 1105 80-81 1.432540322679241 1105 82-83 1.442217794791631 1105 84-85 1.8982323794314766 1105 86-87 1.7948465247693655 1105 88-89 1.8008469749769027 1105 90-91 1.279718387116155 1105 92-93 1.1650322312046804 1105 94-95 1.1272744612233545 1105 96-97 1.1255441675823654 1105 98-99 0.5474222845733046 1105 100-101 0.651716892459536 1106 1 0.09570261886646847 1106 2 0.13100299481230593 1106 3 0.10050246382193251 1106 4 -0.08298349039474573 1106 5 0.04116500633067233 1106 6 0.11718287041111353 1106 7 0.23899349202683595 1106 8 -0.009198403852550996 1106 9 0.14527608403777492 1106 10-11 0.1440132438106403 1106 12-13 -0.10914547798784469 1106 14-15 -0.11581098271634716 1106 16-17 0.190936896318199 1106 18-19 0.3646688783061691 1106 20-21 0.15491024275728194 1106 22-23 0.08220570836065377 1106 24-25 0.07399026922190188 1106 26-27 0.23277108078249142 1106 28-29 0.04274752396450765 1106 30-31 -0.10169970974588693 1106 32-33 -0.10407080742665897 1106 34-35 -0.2831087141878257 1106 36-37 -0.2397797558186454 1106 38-39 -0.4545353234407443 1106 40-41 -0.7059365068952275 1106 42-43 -0.7663227709152238 1106 44-45 -0.7309912854519922 1106 46-47 -0.479108129023075 1106 48-49 -0.4758090201804066 1106 50-51 -0.6888051549020062 1106 52-53 -0.6394245286069804 1106 54-55 -0.3523728483122568 1106 56-57 -0.25166648770942146 1106 58-59 -0.508786295001876 1106 60-61 -0.4798367850084446 1106 62-63 -1.0311362820510226 1106 64-65 -0.7288000791231113 1106 66-67 -0.9580191774503746 1106 68-69 -0.9283671913325939 1106 70-71 -1.22668376054488 1106 72-73 -1.2323487896653198 1106 74-75 -0.8762031842569442 1106 76-77 -0.8377124198802903 1106 78-79 -0.5217859274931413 1106 80-81 -0.40859166867908314 1106 82-83 -0.3479985901060054 1106 84-85 -0.38383956597505886 1106 86-87 0.0031127021981234293 1106 88-89 -0.03616993116486 1106 90-91 0.20935900300723986 1106 92-93 0.4743923354535049 1106 94-95 0.02462023125867674 1106 96-97 -0.08231907441893682 1106 98-99 -0.05001093102286802 1106 100-101 -0.11576364661662453 1107 1 -0.06535523237320007 1107 2 -0.04800526965050267 1107 3 -0.16316695766567335 1107 4 -0.09683473006416676 1107 5 -0.08812424986767553 1107 6 0.050736589419372535 1107 7 0.030431508555757603 1107 8 -0.01075212286081495 1107 9 0.0376727782526558 1107 10-11 -0.2588627892472104 1107 12-13 -0.2722363870787561 1107 14-15 -0.10658784222047046 1107 16-17 -0.13610442599584616 1107 18-19 -0.019033601032674596 1107 20-21 -0.07056083162288473 1107 22-23 0.04409000588131562 1107 24-25 -0.026422953918590508 1107 26-27 -0.2862702415315539 1107 28-29 -0.1914673520685497 1107 30-31 0.10894491835328779 1107 32-33 -0.08707907188946962 1107 34-35 0.1976350874650734 1107 36-37 0.3846499962474752 1107 38-39 0.2795638501129787 1107 40-41 0.6066254765758501 1107 42-43 0.7934127662748551 1107 44-45 1.0805789624818942 1107 46-47 0.5967100527951104 1107 48-49 1.2135298227948041 1107 50-51 1.4667155062550137 1107 52-53 1.2753027441202889 1107 54-55 1.300023845902615 1107 56-57 1.4490773139434765 1107 58-59 1.4731971760725102 1107 60-61 1.7477004050741982 1107 62-63 1.524136445221707 1107 64-65 1.0944230613727512 1107 66-67 1.0904271035413622 1107 68-69 1.12640966817154 1107 70-71 0.9738782229261957 1107 72-73 1.0206264169462465 1107 74-75 1.170193509957933 1107 76-77 1.329841299127974 1107 78-79 1.750660353498592 1107 80-81 1.5165984139655428 1107 82-83 1.5978526495634142 1107 84-85 1.7708215910497351 1107 86-87 1.4912614625287013 1107 88-89 1.36275568866985 1107 90-91 1.3547474327593036 1107 92-93 0.9681774594204455 1107 94-95 1.2689508097710736 1107 96-97 1.1806396032670072 1107 98-99 0.9485841102994463 1107 100-101 0.42142643602800334 1108 1 -0.19316918586157072 1108 2 -0.022113796782285533 1108 3 0.09726715086146243 1108 4 0.10335131644746554 1108 5 0.19370520749666298 1108 6 0.11631798476820876 1108 7 0.16813693491234716 1108 8 0.022674233728338322 1108 9 0.18092859220614343 1108 10-11 0.04735426501635942 1108 12-13 -0.008546464598133241 1108 14-15 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-1.9096634381262128 2316 74-75 -2.2739514175782958 2316 76-77 -2.06923116464014 2316 78-79 -2.079397617515898 2316 80-81 -1.664879846904018 2316 82-83 -1.7336256113061488 2316 84-85 -1.853033481414034 2316 86-87 -1.6656081026886902 2316 88-89 -1.6210124272721806 2316 90-91 -1.518267059994315 2316 92-93 -1.4161993521737557 2316 94-95 -1.0802665815332766 2316 96-97 -1.2817082228199475 2316 98-99 -1.5430100925991042 2316 100-101 -1.2162547233922858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 23.0 6 71.0 7 113.0 8 179.0 9 341.0 10 568.0 11 862.0 12 1333.0 13 1794.0 14 2174.0 15 2883.0 16 3878.0 17 5422.0 18 5108.0 19 3128.0 20 2767.0 21 2834.0 22 2988.0 23 3034.0 24 3342.0 25 3531.0 26 3583.0 27 3750.0 28 4268.0 29 4803.0 30 5580.0 31 6468.0 32 7641.0 33 9343.0 34 13071.0 35 23965.0 36 26079.0 37 47308.0 38 55055.0 39 6706.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.251973201697886 23.672720225269902 16.09912179699108 16.97618477604113 2 13.942529867650514 26.02519716058455 40.08439457874681 19.94787839301813 3 19.079689997651496 32.49456435276025 32.16880430912356 16.2569413404647 4 10.514473594496929 21.945242009409164 39.35863150478413 28.18165289130978 5 9.098932567670968 42.83933969196737 34.56541337434375 13.496314366017925 6 22.24827460814097 42.27721422131986 18.47513996318154 16.99937120735763 7 20.892203728816128 40.58174683141539 22.630474473291464 15.89557496647702 8 22.989946893537073 34.66465655042841 21.591072659641362 20.754323896393153 9 24.818369836135123 20.630838112409943 23.5706331108569 30.980158940598034 10-11 21.414366775505876 30.81917287252176 27.072364184577157 20.694096167395205 12-13 25.34924507003841 30.75951332964644 27.626156258759536 16.26508534155562 14-15 22.10957342237492 31.038858469714558 30.173316691698567 16.678251416211957 16-17 15.353189845223755 34.69472791048281 31.297870406145595 18.654211838147834 18-19 14.776844477693917 32.61053608462202 36.63292266025246 15.979696777431604 20-21 16.592928734308593 30.77599072720248 36.1065235342692 16.524557004219727 22-23 16.096144667762637 31.141145008674307 35.90841597284828 16.854294350714778 24-25 16.359783028659308 32.07581118038773 36.39099538632868 15.173410404624278 26-27 16.185533816928352 30.965548589935416 37.86056554101403 14.9883520521222 28-29 14.46393533284343 31.953840559398177 38.18854688293093 15.39367722482746 30-31 14.978133765670485 32.64517986374561 36.79398768520956 15.582698685374341 32-33 15.037424525943377 32.90820385002917 36.69554314805415 15.358828475973302 34-35 15.63039821801982 31.881496802739644 36.66374973482438 15.824355244416157 36-37 15.856255209517315 31.555846025611885 36.97355459574146 15.61434416912935 38-39 15.713592950260082 31.631957204502314 37.257011664955506 15.397438180282098 40-41 14.830445068672446 32.0931695018951 37.51006267745828 15.566322751974178 42-43 14.698144335364457 32.54620220437124 36.346365477099205 16.409287983165104 44-45 15.04341979412294 32.389745920812146 35.675066526511166 16.891767758553748 46-47 15.938908049569026 32.39392338126976 34.6189613080526 17.048207261108615 48-49 15.742283973999607 32.0959408475886 34.784617948756804 17.377157229654994 50-51 15.553905711406903 32.31520693338586 35.40689702194714 16.7239903332601 52-53 15.175361841233045 33.01704186032417 35.10267162126842 16.70492467717436 54-55 14.760717884226397 32.12429639618482 35.05253827680513 18.062447442783657 56-57 15.999363631542662 32.82960476973311 33.440973037674524 17.730058561049706 58-59 15.951603494124688 31.937110301303857 34.279469365799706 17.83181683877175 60-61 15.611794365722318 32.50610383239227 34.201539169651504 17.68056263223392 62-63 16.063676654418728 32.27849539755294 34.08102579643169 17.576802151596652 64-65 15.745882513371415 32.50503795512053 34.226654949317414 17.52242458219064 66-67 15.263078146899502 32.01428084397136 33.50846622978143 19.214174779347704 68-69 14.970557764170437 32.295736794555886 33.88025750829098 18.853447932982693 70-71 15.762051227660814 31.80838491200691 33.26748687490056 19.16207698543171 72-73 15.577580133182828 30.669361131523726 33.56938310138713 20.18367563390632 74-75 15.172652823127446 31.71520238789688 33.097599224236546 20.014545564739127 76-77 15.812717662576745 30.068228618062083 34.05357702052552 20.065476698835656 78-79 15.42609305012358 29.959185219557007 34.37115881779184 20.24356291252758 80-81 15.31210403404668 30.659815410024226 33.354609351472874 20.67347120445622 82-83 15.108287010293658 30.08210305021828 33.646530743662346 21.163079195825716 84-85 15.211478874839962 29.330904022000166 33.11653876165728 22.341078341502588 86-87 15.292750347902913 29.45950865112258 33.26539588886824 21.982345112106263 88-89 15.944966478683636 28.49235835675199 33.16804009628584 22.39463506827853 90-91 15.280946297394715 28.01421271159374 33.886556858450824 22.81828413256072 92-93 15.395192387821119 28.978439230600234 33.33585860498943 22.290509776589218 94-95 15.09083402146986 28.272562670929325 33.2246835203297 23.411919787271117 96-97 14.909772043727603 27.769528557034523 32.850817051644334 24.46988234759354 98-99 15.31300841494804 27.89546751920968 32.396129464818394 24.395394601023884 100-101 15.371954984336945 26.945192559006355 33.32405292484385 24.35879953181286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4318.0 1 2675.5 2 1138.0 3 1220.5 4 1012.5 5 971.0 6 1698.0 7 3020.0 8 4197.0 9 4250.5 10 3040.5 11 2055.5 12 1790.5 13 1641.0 14 1539.5 15 1425.5 16 1316.5 17 1256.0 18 1253.0 19 1291.5 20 1378.0 21 1499.5 22 1604.5 23 1698.5 24 1816.5 25 2107.5 26 2664.0 27 3178.0 28 3385.5 29 3632.5 30 4250.5 31 4956.5 32 6075.5 33 7493.0 34 8837.5 35 9698.5 36 10870.5 37 11996.0 38 11410.5 39 10373.0 40 9522.0 41 8778.0 42 7926.5 43 7401.5 44 7418.0 45 7186.5 46 6990.0 47 8186.5 48 14473.0 49 13933.5 50 7360.5 51 5215.5 52 3210.5 53 1890.0 54 1231.0 55 953.0 56 785.0 57 677.0 58 537.5 59 426.5 60 364.5 61 318.5 62 268.5 63 203.0 64 159.0 65 132.5 66 114.5 67 96.5 68 85.5 69 61.0 70 33.0 71 30.5 72 26.5 73 28.0 74 32.0 75 29.0 76 15.5 77 6.0 78 4.5 79 4.5 80 3.0 81 0.5 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32083576390730234 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.681861226221411E-4 16-17 0.0015151629936590429 18-19 1.8939537420738036E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0011363722452442822 28-29 0.0 30-31 0.002462139864695945 32-33 0.0 34-35 0.008333396465124735 36-37 0.021969863408056123 38-39 0.017045583678664232 40-41 0.01041674558140592 42-43 0.008522791839332116 44-45 0.002083349116281184 46-47 9.469768710369018E-4 48-49 7.575814968295214E-4 50-51 0.0 52-53 3.787907484147607E-4 54-55 0.0 56-57 0.0 58-59 0.004545488980977129 60-61 0.009280373336161638 62-63 0.0030303259873180857 64-65 7.575814968295214E-4 66-67 0.0030303259873180857 68-69 0.002462139864695945 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.20663035326025198 78-79 1.8939537420738036E-4 80-81 0.0028409306131107056 82-83 1.8939537420738036E-4 84-85 0.0 86-87 0.10416745581405919 88-89 0.3905332616156183 90-91 0.37651800392427215 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0013257676194516625 100-101 3.787907484147607E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 263998.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.485094584049875 #Duplication Level Percentage of deduplicated Percentage of total 1 73.62406716417911 23.916847854907992 2 11.56133395522388 7.511420541064704 3 4.520755597014926 4.405715194812082 4 2.419542910447761 3.143963211842514 5 1.5555037313432836 2.526534291926454 6 1.0272854477611941 2.0022878961204253 7 0.7054570895522388 1.6041788195365119 8 0.5655317164179104 1.4697081038492716 9 0.4640858208955224 1.356828460821673 >10 3.091184701492537 19.62022439563936 >50 0.29617537313432835 6.619747119296358 >100 0.13759328358208955 8.547792028727489 >500 0.017490671641791043 4.0220001666679295 >1k 0.010494402985074626 6.503079568784612 >5k 0.003498134328358209 6.749672346002621 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6684 2.5318373624042607 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5994 2.270471745998076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5141 1.9473632376002847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3813 1.4443291237054825 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2349 0.8897794680262728 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2069 0.78371805847014 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2069 0.78371805847014 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1707 0.6465958075439966 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1646 0.6234895718906962 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1226 0.4643974575564967 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1149 0.4352305699285601 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1140 0.4318214531928272 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 988 0.37424525943378356 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 889 0.3367449753407223 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 887 0.33598739384389276 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 878 0.3325782771081599 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 810 0.3068205062159562 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 766 0.2901537132857067 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 761 0.28825975954363287 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 675 0.2556837551799635 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 624 0.2363654270108107 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 605 0.22916840279093026 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 594 0.22500170455836788 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 564 0.21363798210592505 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 537 0.2034106318987265 RNA PCR Primer, Index 26 (100% over 22bp) TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 520 0.19697118917567555 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 520 0.19697118917567555 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 496 0.1878802112137213 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 495 0.18750142046530655 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 464 0.17575890726444898 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 463 0.17538011651603422 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 439 0.16628913855407995 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 435 0.1647739755604209 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 431 0.16325881256676186 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 425 0.1609860680762733 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 405 0.1534102531079781 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 399 0.15113750861748954 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 387 0.1465920196365124 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 368 0.13939499541663194 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 339 0.12841006371260388 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 335 0.12689490071894483 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 334 0.1265161099705301 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 317 0.12007666724747913 RNA PCR Primer, Index 26 (100% over 24bp) AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 305 0.11553117826650203 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 296 0.11212206153076917 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 293 0.1109856892855249 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 292 0.11060689853711013 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 290 0.10984931704028061 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 280 0.106061409556133 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 264 0.10000075758149682 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8939537420738036E-4 0.0 20-21 0.0 0.0 0.0 0.002651535238903325 0.0 22-23 0.0 0.0 0.0 0.008333396465124735 0.0 24-25 0.0 0.0 0.0 0.01628800218183471 0.0 26-27 0.0 0.0 0.0 0.03314419048629156 0.0 28-29 0.0 0.0 0.0 0.07121266070197502 0.0 30-31 0.0 0.0 0.0 0.15037992712066 0.0 32-33 0.0 0.0 0.0 0.26250198865142915 0.0 34-35 0.0 0.0 0.0 0.4155334510109925 0.0 36-37 0.0 0.0 0.0 0.6276562701232585 0.0 38-39 0.0 0.0 0.0 0.8797415131932818 0.0 40-41 0.0 0.0 0.0 1.1371298267411118 0.0 42-43 0.0 0.0 0.0 1.3750104167455814 0.0 44-45 0.0 0.0 0.0 1.633724497912863 0.0 46-47 0.0 0.0 0.0 1.964408821278949 0.0 48-49 0.0 0.0 0.0 2.289790074167229 0.0 50-51 0.0 0.0 0.0 2.6119516056939824 0.0 52-53 0.0 0.0 0.0 2.8950976901340164 0.0 54-55 0.0 0.0 0.0 3.230706293229494 0.0 56-57 0.0 0.0 0.0 3.598890900688642 0.0 58-59 0.0 0.0 0.0 3.9833635103296237 0.0 60-61 0.0 0.0 0.0 4.441889711285691 0.0 62-63 0.0 0.0 0.0 4.882233956317851 0.0 64-65 0.0 0.0 0.0 5.343411692512822 0.0 66-67 0.0 0.0 0.0 5.83886999143933 0.0 68-69 0.0 0.0 0.0 6.4169046735202535 0.0 70-71 0.0 0.0 0.0 7.049864014121319 0.0 72-73 0.0 0.0 0.0 7.668808097031038 0.0 74-75 0.0 0.0 0.0 8.351010234926022 0.0 76-77 0.0 0.0 0.0 9.011810695535573 0.0 78-79 0.0 0.0 0.0 9.768634610868265 0.0 80-81 0.0 0.0 0.0 10.5779210448564 0.0 82-83 0.0 0.0 0.0 11.362207289449163 0.0 84-85 0.0 0.0 0.0 12.249714012984947 0.0 86-87 0.0 0.0 0.0 13.168849764013363 0.0 88-89 0.0 0.0 0.0 14.026810809172797 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGAGC 25 0.005036173 56.945103 3 GTATGGT 65 1.3122766E-4 36.600334 1 GTGGTAC 65 1.3329489E-4 36.50327 6 TCGAAAT 35 2.0634798E-4 33.895897 24-25 AGGACAG 85 1.638231E-5 33.49712 5 TGGTACT 75 3.0894842E-4 31.63617 7 TAATACC 110 2.7434708E-6 30.19816 4 GATCAGT 40 4.515794E-4 29.658909 14-15 TACAGAG 65 0.0058635697 29.202614 5 CTACACG 115 3.878742E-6 28.885197 4 CGGAGGT 115 3.878742E-6 28.885197 9 CATATAG 100 4.9714057E-5 28.472551 3 TTAGGCA 85 6.4301357E-4 27.914265 4 GGCCGAC 120 5.4014236E-6 27.681648 7 GCCGACT 120 5.4014236E-6 27.681648 8 ACGGAGG 120 5.4014236E-6 27.681648 8 TACACGG 120 5.4014236E-6 27.681648 5 TAGGCAT 70 0.008419546 27.116716 5 AGGCCGA 125 7.41803E-6 26.574383 6 CCGACTA 125 7.41803E-6 26.574383 9 >>END_MODULE