##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939088_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 259897 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.009553784768585 33.0 31.0 34.0 31.0 34.0 2 32.050431517101 34.0 31.0 34.0 30.0 34.0 3 32.05804222441967 34.0 31.0 34.0 30.0 34.0 4 35.51571584127558 37.0 35.0 37.0 35.0 37.0 5 35.572195908379086 37.0 35.0 37.0 35.0 37.0 6 35.712178286013305 37.0 37.0 37.0 35.0 37.0 7 35.679476869682986 37.0 36.0 37.0 35.0 37.0 8 35.69254358457389 37.0 37.0 37.0 35.0 37.0 9 37.32214685048307 39.0 38.0 39.0 35.0 39.0 10-11 37.26373524896401 39.0 38.0 39.0 35.0 39.0 12-13 37.18506754598937 39.0 37.5 39.0 34.5 39.0 14-15 38.34230098846851 40.0 38.0 41.0 34.0 41.0 16-17 38.280072490255755 40.0 38.0 41.0 34.0 41.0 18-19 38.28360850644678 40.0 38.0 41.0 34.0 41.0 20-21 38.28629418577359 40.0 38.0 41.0 34.0 41.0 22-23 38.248581168693754 40.0 38.0 41.0 34.0 41.0 24-25 38.14018245689638 40.0 38.0 41.0 34.0 41.0 26-27 37.9335929233504 40.0 37.5 41.0 34.0 41.0 28-29 37.728823341554545 40.0 37.0 41.0 34.0 41.0 30-31 37.35752625078396 40.0 35.5 41.0 33.0 41.0 32-33 36.94239256320773 39.5 35.0 41.0 33.0 41.0 34-35 36.548746234085044 39.0 35.0 41.0 32.5 41.0 36-37 36.19256282296448 39.0 35.0 41.0 31.0 41.0 38-39 35.9132060008388 39.0 35.0 41.0 30.5 41.0 40-41 35.64973624166497 39.0 35.0 41.0 30.0 41.0 42-43 35.31599633701043 38.0 35.0 41.0 29.5 41.0 44-45 34.859400070027746 38.0 35.0 41.0 25.0 41.0 46-47 34.28260426245782 38.5 35.0 41.0 17.5 41.0 48-49 33.91258075314451 39.0 35.0 41.0 5.5 41.0 50-51 32.7401278198671 38.0 33.5 40.0 2.0 40.5 52-53 32.852716653135666 38.0 33.5 39.5 2.0 40.5 54-55 33.27783121775165 38.0 35.0 40.5 2.0 41.0 56-57 33.16145434537528 38.0 35.0 41.0 2.0 41.0 58-59 32.945387980623096 38.0 35.0 41.0 2.0 41.0 60-61 32.61399708345999 38.0 34.0 40.5 2.0 41.0 62-63 32.21783629668676 37.5 33.5 40.0 2.0 41.0 64-65 31.80991892942204 37.0 33.0 40.0 2.0 41.0 66-67 31.36225697103083 36.5 33.0 39.5 2.0 41.0 68-69 30.90621476969723 36.0 32.0 39.0 2.0 41.0 70-71 30.396607117434982 35.0 31.0 39.0 2.0 41.0 72-73 29.859024921411176 35.0 31.0 38.0 2.0 40.0 74-75 29.359396222349623 35.0 30.0 37.0 2.0 40.0 76-77 28.8294382005179 35.0 29.0 37.0 2.0 39.0 78-79 28.313697349334543 35.0 28.5 36.0 2.0 39.0 80-81 27.791752117184885 35.0 26.5 36.0 2.0 38.5 82-83 27.342656898694482 35.0 26.0 35.5 2.0 37.0 84-85 26.872170513703505 34.0 24.5 35.0 2.0 37.0 86-87 26.399323578186745 34.0 21.5 35.0 2.0 36.0 88-89 25.85901337837682 34.0 16.0 35.0 2.0 36.0 90-91 25.344490317318012 34.0 4.5 35.0 2.0 36.0 92-93 24.899625620919057 34.0 2.0 35.0 2.0 35.0 94-95 24.40832329730624 33.5 2.0 35.0 2.0 35.0 96-97 23.797415899375522 33.0 2.0 35.0 2.0 35.0 98-99 23.1956217270688 33.0 2.0 35.0 2.0 35.0 100-101 20.886426161133066 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32241773136981067 1101 2 0.34828797649139887 1101 3 0.28588404270715273 1101 4 0.635344769152347 1101 5 0.15857054455623398 1101 6 0.30914083168719486 1101 7 0.4039839601633872 1101 8 0.12921638579198458 1101 9 -0.10203638890478572 1101 10-11 0.30305483618496254 1101 12-13 0.026120884380958387 1101 14-15 0.025183030365433012 1101 16-17 0.04705094008011912 1101 18-19 0.295661429277537 1101 20-21 0.4074776925560286 1101 22-23 0.052133979679112485 1101 24-25 0.08750257698046937 1101 26-27 0.4005857102499846 1101 28-29 0.38086782634955085 1101 30-31 0.3572979279406425 1101 32-33 0.2942039971807091 1101 34-35 0.3798992704323183 1101 36-37 0.20642991805325295 1101 38-39 0.22903467875053707 1101 40-41 0.46890893296804137 1101 42-43 0.7536320629452149 1101 44-45 0.9565205990929471 1101 46-47 0.2887804806896881 1101 48-49 0.28114764386770474 1101 50-51 0.24083522355656584 1101 52-53 0.0761935329697252 1101 54-55 0.3690746732848851 1101 56-57 0.1510342994467564 1101 58-59 0.13276103855954346 1101 60-61 0.12200956507645344 1101 62-63 0.20701620402055454 1101 64-65 0.2127127426507549 1101 66-67 0.6644019466055191 1101 68-69 0.2238939343429429 1101 70-71 0.024683737382979132 1101 72-73 0.2841530839334503 1101 74-75 0.18820036762472014 1101 76-77 -0.028883064735985897 1101 78-79 -0.1457546093318669 1101 80-81 -0.012885836073458279 1101 82-83 0.1342819100598831 1101 84-85 0.3287515599946609 1101 86-87 0.3376710300262502 1101 88-89 0.3282175854981837 1101 90-91 0.06527158350048978 1101 92-93 -0.29943260496659363 1101 94-95 -0.2189993590911712 1101 96-97 -0.2941511742289684 1101 98-99 -0.12419171874231338 1101 100-101 0.05846450003157955 1102 1 0.08803473085777824 1102 2 0.37354825981619655 1102 3 0.4126548227037361 1102 4 0.4927223079814951 1102 5 0.28246114022102375 1102 6 0.2075449962203919 1102 7 0.33042191885941463 1102 8 0.44326315145165296 1102 9 0.5982110936007601 1102 10-11 0.7410668689550661 1102 12-13 0.6962331048964003 1102 14-15 0.6869747183667982 1102 16-17 0.7201820204692595 1102 18-19 0.6296668056216177 1102 20-21 0.5057773170653306 1102 22-23 0.8916650430005078 1102 24-25 0.9197585080267245 1102 26-27 0.9231471541823879 1102 28-29 0.7219776573787371 1102 30-31 0.8005156272067353 1102 32-33 0.7377203822293552 1102 34-35 0.1390518561978098 1102 36-37 0.26175079192251616 1102 38-39 0.36885162703521956 1102 40-41 0.6038615699368108 1102 42-43 0.6384246896733288 1102 44-45 0.3795130039401826 1102 46-47 0.30545354776598543 1102 48-49 0.46722888640056226 1102 50-51 0.2662768518207699 1102 52-53 0.2518527751612254 1102 54-55 0.351132191632729 1102 56-57 0.10369260291150084 1102 58-59 -0.06620636202075758 1102 60-61 0.0904555456192071 1102 62-63 0.20656817534671745 1102 64-65 0.2079871068767325 1102 66-67 0.4398329075203513 1102 68-69 0.3886404781217436 1102 70-71 0.41302859145364224 1102 72-73 0.4998255536381784 1102 74-75 -0.06635459055926773 1102 76-77 -0.03692624616626361 1102 78-79 0.18116764702587318 1102 80-81 -0.053389761658028334 1102 82-83 0.06470945742291434 1102 84-85 0.1998793121707969 1102 86-87 0.33395879244304183 1102 88-89 0.3775540763669305 1102 90-91 0.12719554663413035 1102 92-93 0.23571631123071413 1102 94-95 0.03865979776153594 1102 96-97 -0.025685992457336937 1102 98-99 0.3901878682179216 1102 100-101 -0.2910512023067042 1103 1 0.16932932820742508 1103 2 0.2957365031053598 1103 3 0.21990932491236492 1103 4 0.4968422536152062 1103 5 0.48994500022782006 1103 6 0.5749422099478849 1103 7 0.3621922212719184 1103 8 0.3318122510099286 1103 9 0.3325807843924693 1103 10-11 0.10071263857789603 1103 12-13 0.3162849227693201 1103 14-15 0.48184814684114485 1103 16-17 0.4511962916211516 1103 18-19 0.24224325822100923 1103 20-21 0.2619147618495603 1103 22-23 0.605626137122151 1103 24-25 0.5060012875034445 1103 26-27 0.5525431786472268 1103 28-29 0.444263590100455 1103 30-31 0.7484216754568109 1103 32-33 0.5749400560315934 1103 34-35 0.7492710203160584 1103 36-37 1.1401486852286595 1103 38-39 1.1753628400831317 1103 40-41 1.261799898173301 1103 42-43 1.2232870749944311 1103 44-45 0.9158492254828303 1103 46-47 0.4213429463388678 1103 48-49 0.7252139356700127 1103 50-51 0.48168205059753433 1103 52-53 0.8470999718992402 1103 54-55 0.6483204349218923 1103 56-57 0.7461068061055229 1103 58-59 0.6716461694097546 1103 60-61 0.5202465751808774 1103 62-63 0.10654269114692028 1103 64-65 0.29996383130758275 1103 66-67 0.11397493946054738 1103 68-69 -0.037965026465411 1103 70-71 -0.14908914554941788 1103 72-73 0.27000054514344285 1103 74-75 0.3968419775684886 1103 76-77 0.5845909131949334 1103 78-79 0.9202306779467015 1103 80-81 0.5501822736800612 1103 82-83 0.1298215879020148 1103 84-85 -0.06190713023491057 1103 86-87 0.1601565498334594 1103 88-89 -0.06011581829837809 1103 90-91 0.28373224388183615 1103 92-93 0.36298865237920097 1103 94-95 0.23442092586211416 1103 96-97 -0.07850199653480772 1103 98-99 -0.25999901591391605 1103 100-101 -0.3314648958167261 1104 1 0.09958831984499739 1104 2 0.12215527390993941 1104 3 -0.15855133921039055 1104 4 -0.027230166616995177 1104 5 0.07961781016530267 1104 6 0.07242536331315108 1104 7 0.06772116500821568 1104 8 0.09599995905571745 1104 9 -0.29797048620913813 1104 10-11 -0.44692477312950274 1104 12-13 -0.29776476465456625 1104 14-15 -0.4019100735047161 1104 16-17 -0.3116518045061625 1104 18-19 -0.17693348936363407 1104 20-21 -0.3584270421611535 1104 22-23 -0.33967304287298106 1104 24-25 -0.3501325113111946 1104 26-27 -0.07224370127482871 1104 28-29 -0.3187671803793677 1104 30-31 -0.3556012197287046 1104 32-33 -0.2398484476624816 1104 34-35 -0.21210350270091283 1104 36-37 -0.0260646784150822 1104 38-39 -0.44045269941511833 1104 40-41 -0.33351551954204695 1104 42-43 0.07205300825739869 1104 44-45 -0.009396370035553048 1104 46-47 0.06129179752016256 1104 48-49 0.22919217712491502 1104 50-51 -0.022255596556803425 1104 52-53 -0.08744171844742965 1104 54-55 0.03668610838133901 1104 56-57 0.1556018130065624 1104 58-59 0.2977726612502991 1104 60-61 0.3093591837706313 1104 62-63 -0.0678910183602639 1104 64-65 -0.41256762927535107 1104 66-67 -0.7114289738300279 1104 68-69 -0.6467577519667529 1104 70-71 -0.3622295206407564 1104 72-73 -0.08428621299571404 1104 74-75 -0.29695521330591035 1104 76-77 -0.4574850793357186 1104 78-79 -0.5994575578249943 1104 80-81 -0.8392591487442331 1104 82-83 -0.9885107982309691 1104 84-85 -0.837055299431956 1104 86-87 -1.0507511387270654 1104 88-89 -1.1271370085232704 1104 90-91 -1.20410976310038 1104 92-93 -1.6460232823451655 1104 94-95 -1.3578905136117854 1104 96-97 -0.9507720298221329 1104 98-99 -1.055403741090501 1104 100-101 -0.8819845028638973 1105 1 -0.04648734580403158 1105 2 0.040538394055239735 1105 3 0.20470560711260077 1105 4 0.35009332528701265 1105 5 0.2890315752849091 1105 6 0.21017317024594462 1105 7 0.2715508206870041 1105 8 0.22524600767032155 1105 9 0.15542442046474036 1105 10-11 0.2295736617740829 1105 12-13 0.38013452815372517 1105 14-15 0.567261391502818 1105 16-17 0.25772447558813383 1105 18-19 0.38194118698989143 1105 20-21 0.17039198631973562 1105 22-23 0.27674307955233957 1105 24-25 0.492710553959121 1105 26-27 0.29150596058705247 1105 28-29 0.12909192229868438 1105 30-31 0.21179453712100837 1105 32-33 0.35056002318034984 1105 34-35 0.22826825600373013 1105 36-37 0.2440207256116551 1105 38-39 -0.23561829048053795 1105 40-41 -0.8249654557788588 1105 42-43 -1.0558984544314711 1105 44-45 -1.0211925597020581 1105 46-47 -1.0789775536411028 1105 48-49 -1.2311780358054918 1105 50-51 -1.3440761321675794 1105 52-53 -1.1696962301759903 1105 54-55 -1.3557859710714624 1105 56-57 -1.808748768591002 1105 58-59 -1.7090577286712545 1105 60-61 -1.9138506164380473 1105 62-63 -1.6809257596363025 1105 64-65 -1.943405825356809 1105 66-67 -1.8461528599064643 1105 68-69 -2.0338486048476874 1105 70-71 -2.097979414368776 1105 72-73 -1.648261944340149 1105 74-75 -2.0702867432359646 1105 76-77 -1.918193430381045 1105 78-79 -2.171442331948736 1105 80-81 -1.9561776477974426 1105 82-83 -2.257282294153992 1105 84-85 -1.6951049187850487 1105 86-87 -1.7665991891193045 1105 88-89 -1.5533552718459127 1105 90-91 -1.2862869861169592 1105 92-93 -1.4745679358985129 1105 94-95 -1.2947746191881642 1105 96-97 -1.2433291268581108 1105 98-99 -1.0686104497507039 1105 100-101 -1.308589209746458 1106 1 0.06778990976550148 1106 2 0.010710846131324558 1106 3 -0.2703332501148452 1106 4 -0.05396632980883709 1106 5 -0.04671337327552294 1106 6 0.19451925861838504 1106 7 0.08999505131389895 1106 8 0.15528449482893336 1106 9 0.23349044074451797 1106 10-11 -0.11686627605435262 1106 12-13 0.10179650728183987 1106 14-15 0.2561445237654212 1106 16-17 0.11472062687227691 1106 18-19 0.32846624064987395 1106 20-21 0.16628422227562822 1106 22-23 0.3807971836156909 1106 24-25 0.3181028269218942 1106 26-27 0.39807653271346766 1106 28-29 0.16512737489286877 1106 30-31 0.3256647169744582 1106 32-33 0.581529233453459 1106 34-35 0.38175430440873015 1106 36-37 0.40118895890305595 1106 38-39 0.5582348662065968 1106 40-41 0.5797325680535721 1106 42-43 0.6320687574699377 1106 44-45 0.634652677512058 1106 46-47 0.5136691786511065 1106 48-49 0.6575145110082161 1106 50-51 0.7793418235721887 1106 52-53 0.55752603316499 1106 54-55 0.7446507101512481 1106 56-57 0.9764170222460891 1106 58-59 0.8914992249221214 1106 60-61 0.859287937332958 1106 62-63 0.7698920924841914 1106 64-65 0.5830263410261303 1106 66-67 0.27089066018827523 1106 68-69 0.4860981052403872 1106 70-71 0.5171952581203065 1106 72-73 0.49339115313598114 1106 74-75 0.20385099638360415 1106 76-77 0.15124073437359797 1106 78-79 0.21361428858268638 1106 80-81 0.23596209019382286 1106 82-83 0.35049100167903546 1106 84-85 0.605835056050271 1106 86-87 0.5801923141002945 1106 88-89 0.49568810022129384 1106 90-91 -0.022168860145530545 1106 92-93 0.2100964116701327 1106 94-95 0.5736566590295844 1106 96-97 0.40592888513211633 1106 98-99 0.10244461649162062 1106 100-101 -0.09444887964506066 1107 1 -0.006499319321427777 1107 2 9.377460766515355E-4 1107 3 -0.10063669462003944 1107 4 -0.03804232762184512 1107 5 -0.005023925489851422 1107 6 -0.004223223612342508 1107 7 -0.15822802140343128 1107 8 -0.03553015798681258 1107 9 -0.17807325526423057 1107 10-11 -0.1301248873173435 1107 12-13 0.0762360917542324 1107 14-15 -0.1234344811553072 1107 16-17 0.22130209215384866 1107 18-19 -0.04304824814181529 1107 20-21 -0.071208943388676 1107 22-23 0.07834365709847191 1107 24-25 -0.028568851590954125 1107 26-27 0.1321525305264828 1107 28-29 0.1386443240454085 1107 30-31 0.11824262840147526 1107 32-33 0.21845514925443865 1107 34-35 0.4513278418608877 1107 36-37 0.5938147106799789 1107 38-39 0.4479286879671278 1107 40-41 0.8997845090049097 1107 42-43 0.7775375928991366 1107 44-45 0.902661152088335 1107 46-47 0.8809463793072041 1107 48-49 0.9847766761257617 1107 50-51 0.5720564217132491 1107 52-53 1.0027488325088996 1107 54-55 0.9361829682157605 1107 56-57 1.032082686543525 1107 58-59 1.17020343487291 1107 60-61 1.183645509776916 1107 62-63 1.1283871717624905 1107 64-65 1.1487726504848546 1107 66-67 1.1511530986792557 1107 68-69 1.0499308007023878 1107 70-71 1.1502597742493386 1107 72-73 0.9126093051316033 1107 74-75 0.901910045044076 1107 76-77 0.33546025323636286 1107 78-79 0.3741186625575317 1107 80-81 0.6371717676131752 1107 82-83 0.897818776419328 1107 84-85 0.6046732189808317 1107 86-87 0.393546606063115 1107 88-89 0.5525839449451873 1107 90-91 0.8308245788921944 1107 92-93 0.8503369802868654 1107 94-95 1.0137195349180494 1107 96-97 0.6427727889046686 1107 98-99 0.3968336268798076 1107 100-101 0.6224045921974799 1108 1 -0.11061511420640713 1108 2 -0.016299755529828985 1108 3 0.02291747579507586 1108 4 0.02346968882847733 1108 5 0.0037795702047915825 1108 6 0.006829340325452904 1108 7 0.1698789057267618 1108 8 0.21543372840310582 1108 9 0.47717671260622296 1108 10-11 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-1.4461707948229616 2316 74-75 -1.13778781956292 2316 76-77 -1.0352178476268818 2316 78-79 -1.4440605741530526 2316 80-81 -1.352770304172374 2316 82-83 -1.2282091654252838 2316 84-85 -1.40808234667114 2316 86-87 -1.3745701590436568 2316 88-89 -1.7017038787713084 2316 90-91 -1.5286143125207978 2316 92-93 -1.8465280810488913 2316 94-95 -1.9550673801205711 2316 96-97 -1.7192501552116397 2316 98-99 -2.3541261641242777 2316 100-101 -2.382763513209156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2295.0 3 342.0 4 310.0 5 286.0 6 324.0 7 348.0 8 422.0 9 491.0 10 667.0 11 958.0 12 1500.0 13 2518.0 14 2413.0 15 2907.0 16 3837.0 17 5528.0 18 4396.0 19 2644.0 20 2637.0 21 2629.0 22 2518.0 23 2747.0 24 2984.0 25 3182.0 26 3466.0 27 3683.0 28 4063.0 29 4605.0 30 5357.0 31 6029.0 32 7173.0 33 8821.0 34 11981.0 35 23317.0 36 25129.0 37 41177.0 38 54231.0 39 11980.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.903041913207375 23.375921148171606 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33.0004775254937 36.46099694999846 15.617779352413812 20-21 16.742593740733753 31.324250324439017 35.663261180149334 16.269894754677892 22-23 16.10437126901598 31.57770075101098 35.56422106682072 16.753706913152318 24-25 16.468870733520536 32.19181775810732 36.127706453116396 15.211605055255747 26-27 16.321051425313787 31.185877250254713 37.41715923338707 15.075912091044433 28-29 14.804151348186723 32.53423668231093 37.43261955154809 15.228992417954254 30-31 15.201651415719258 33.07433681486274 36.427234494870135 15.296777274547862 32-33 14.965011803942863 33.512606919074635 36.060371487219776 15.462009789762726 34-35 15.769774951888463 32.23019416407531 35.95944153941479 16.04058934462145 36-37 15.93015677788285 32.079806459473104 36.47681980889613 15.513216953747913 38-39 15.917185161696978 32.47454572874815 36.392163990085116 15.216105119469761 40-41 14.940040359680525 32.76054029396074 36.853916512676186 15.44550283368255 42-43 14.79717018524498 33.15345693310415 35.58207835547469 16.467294526176175 44-45 15.33306003743638 32.86782791640239 34.9848398125087 16.81427223365252 46-47 15.971776407892396 33.01789015306794 34.10286257918307 16.9074708598566 48-49 15.86025923899066 32.517805035107294 34.49970897010224 17.12222675579981 50-51 15.631580677535439 32.87530045113596 34.89429341047571 16.598825460852883 52-53 15.231776553943257 33.508013365923084 34.585099470932214 16.67511060920145 54-55 14.78577478024464 32.554537614615036 34.892665024382886 17.767022580757434 56-57 16.03913275444796 32.99395340565535 33.44590153793813 17.52101230195857 58-59 16.286540438987807 31.850196763643957 34.13868551061905 17.724577286749184 60-61 15.875827801775767 32.052446120439846 34.58511386379618 17.48661221398821 62-63 16.37335209927907 31.731332491701288 34.58450378831085 17.310811620708787 64-65 16.040904810066852 32.10559598377886 34.68322558561834 17.170273620535948 66-67 15.62850534165719 31.90044636295419 33.78955959370914 18.68148870167947 68-69 15.3109029784633 32.23483397746133 34.20803206325995 18.24623098081543 70-71 16.27385633683494 31.943458523291056 33.508445825056434 18.27423931481757 72-73 16.15708616648488 30.96276871427135 33.93225963658305 18.947885482660727 74-75 15.859333297231226 32.21320099332369 33.43899646457223 18.488469244872856 76-77 16.541369375640507 30.789682309492044 33.82350097646808 18.845447338399364 78-79 16.364659125176875 30.683760022887363 34.29567227767941 18.655908574256355 80-81 16.497059585442145 31.56141619335207 33.28448752498676 18.657036696219027 82-83 16.37377489128943 31.042596756760936 33.52249327628244 19.0611350756672 84-85 16.597418114957108 30.491145916913336 32.990667396209346 19.92076857192021 86-87 16.946291856778284 30.741435310494165 33.08734823292862 19.224924599798932 88-89 17.83810435337452 29.84309289712579 32.90698686156791 19.411815887931784 90-91 17.42989036576333 29.678214135835056 33.3787805826144 19.513114915787213 92-93 17.909272887272866 30.667930257521103 32.78263980891411 18.640157046291908 94-95 17.86444342591035 30.038697849281448 32.53075425028477 19.56610447452343 96-97 17.80312993161494 29.445415725469566 32.3285731967695 20.42288114614599 98-99 18.58279229256236 29.482515534977633 31.76175252727445 20.172939645185558 100-101 19.43746229640056 28.500634193877712 32.46956642794166 19.592337081780073 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5062.0 1 3075.5 2 1260.0 3 1360.5 4 1093.5 5 1056.0 6 1748.5 7 3001.0 8 4066.0 9 4054.5 10 2947.5 11 2087.5 12 1915.5 13 1783.5 14 1659.5 15 1565.5 16 1440.5 17 1387.5 18 1369.0 19 1355.0 20 1468.0 21 1604.0 22 1735.0 23 1913.5 24 2079.0 25 2374.5 26 2973.0 27 3528.5 28 3752.5 29 3995.5 30 4510.0 31 5046.5 32 6001.0 33 7433.0 34 8686.5 35 9320.5 36 10365.0 37 11314.5 38 10787.5 39 9858.5 40 8970.0 41 8200.0 42 7394.5 43 6952.0 44 6868.5 45 6546.5 46 6461.5 47 7732.0 48 14287.0 49 13979.5 50 6627.5 51 3849.5 52 2345.5 53 1668.5 54 1295.5 55 1065.0 56 853.0 57 733.5 58 648.0 59 555.0 60 477.0 61 420.0 62 395.5 63 332.5 64 246.0 65 225.0 66 215.0 67 191.5 68 158.5 69 112.0 70 91.0 71 81.5 72 77.0 73 79.0 74 67.0 75 48.5 76 34.5 77 28.0 78 26.0 79 18.0 80 9.5 81 6.5 82 5.0 83 4.0 84 3.5 85 3.0 86 1.5 87 0.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.377457223438516 2 0.116584646994771 3 0.11889325386595459 4 0.1327448950930561 5 0.13428396634051182 6 0.07041250957109932 7 0.0827250795507451 8 0.07964693705583366 9 0.07695356237278615 10-11 0.07656879456092222 12-13 0.08118600830328938 14-15 0.07291350034821487 16-17 0.07560687503126239 18-19 0.08657275766938441 20-21 0.0838793829863369 22-23 0.09503764953039089 24-25 0.11004359419308418 26-27 0.11062074591088007 28-29 0.10407969310919325 30-31 0.0927290426592073 32-33 0.0915747392236155 34-35 0.3322470055445042 36-37 0.5180898586747827 38-39 0.4986590841756542 40-41 0.4705710339095873 42-43 0.4976971646459944 44-45 0.5098173507197082 46-47 0.5075087438485246 48-49 0.5127031093086877 50-51 0.510779270249368 52-53 0.5113564219671639 54-55 0.5055849047892049 56-57 0.47672731889941017 58-59 0.4659538201672201 60-61 0.41516446900118126 62-63 0.48961704059685185 64-65 0.5177050908629187 66-67 0.5244385275705375 68-69 0.5417530791044145 70-71 0.5373282492679792 72-73 0.5198213138281704 74-75 0.527516670065449 76-77 0.5048153691654771 78-79 0.47672731889941017 80-81 0.4848074429485527 82-83 0.4992362358934501 84-85 0.4953885577748108 86-87 0.49134849575023953 88-89 0.5019296105764977 90-91 0.5215527689815581 92-93 0.5288633574069728 94-95 0.5215527689815581 96-97 0.5236689919468097 98-99 0.5261699827239252 100-101 0.5005829232349738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 259897.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.74953539286717 #Duplication Level Percentage of deduplicated Percentage of total 1 75.35349285263473 26.184988668587938 2 11.007274700209273 7.649953635478671 3 4.189872997242921 4.367884200279342 4 2.2267004750146713 3.0950722786334586 5 1.389611683810747 2.414418019446165 6 0.9522438630097549 1.9854019092178825 7 0.6477472789077984 1.575624189582796 8 0.5480938513835217 1.5236805349811655 9 0.3963991894854561 1.2397218898255848 >10 2.8810913157574216 19.411920876347168 >50 0.2557771306456435 6.010457989126461 >100 0.12401315425243321 7.993166523661296 >500 0.014394383975728852 3.4159686337279767 >1k 0.011072603058252964 8.10898163503234 >5k 0.002214520611650593 5.022759016071752 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6630 2.551010592657861 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6424 2.471748423413891 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4612 1.7745491483164484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3524 1.355921769008492 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2448 0.9419116034429024 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1910 0.7349065206601076 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1885 0.7252873253635094 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1531 0.5890795199636779 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1434 0.5517570422128766 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1273 0.4898094245027838 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1252 0.4817293004536412 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1206 0.4640299811079004 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 910 0.3501387087961769 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 902 0.3470605663012655 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 847 0.32589833664874934 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 806 0.31012285636232817 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 720 0.2770328245420301 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 679 0.2612573442556089 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 678 0.260872576443745 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 632 0.24317325709800422 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 593 0.2281673124353109 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 547 0.21046799308957012 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 526 0.20238786904042758 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 523 0.20123356560483577 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 515 0.1981554231099243 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 456 0.1754541222099524 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 456 0.1754541222099524 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 448 0.17237597971504096 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 445 0.17122167627944918 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 444 0.17083690846758523 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.16583492691335414 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 395 0.15198328568625263 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 373 0.14351839382524617 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 373 0.14351839382524617 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 361 0.138901180082879 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 357 0.13736210883542327 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 351 0.13505350196423968 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 349 0.13428396634051182 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 334 0.12851244916255286 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 308 0.11850848605409066 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 293 0.11273696887613169 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 287 0.11042836200494811 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 284 0.10927405856935632 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 281 0.10811975513376454 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 274 0.10542638045071702 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 264 0.10157870233207772 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.8476781186393074E-4 0.0 16-17 0.0 0.0 0.0 5.771517177958961E-4 0.0 18-19 0.0 0.0 0.0 7.695356237278615E-4 0.0 20-21 0.0 0.0 0.0 0.001539071247455723 0.0 22-23 0.0 0.0 0.0 0.0071182045194827185 0.0 24-25 0.0 0.0 0.0 0.016737399816080984 0.0 26-27 0.0 0.0 0.0 0.038861548998257 0.0 28-29 0.0 0.0 0.0 0.07560687503126239 0.0 30-31 0.0 0.0 0.0 0.150059446626933 0.0 32-33 0.0 0.0 0.0 0.244327560533596 0.0 34-35 0.0 0.0 0.0 0.39784991746730436 0.0 36-37 0.0 0.0 0.0 0.6117808208636499 0.0 38-39 0.0 0.0 0.0 0.8622646663870688 0.0 40-41 0.0 0.0 0.0 1.1115942084748958 0.0 42-43 0.0 0.0 0.0 1.3464949576178253 3.8476781186393074E-4 44-45 0.0 0.0 0.0 1.6115999799920737 3.8476781186393074E-4 46-47 0.0 0.0 0.0 1.9413459947594625 3.8476781186393074E-4 48-49 0.0 0.0 0.0 2.2745549198336263 3.8476781186393074E-4 50-51 0.0 0.0 0.0 2.5902569094679815 3.8476781186393074E-4 52-53 0.0 0.0 0.0 2.8732536350939024 3.8476781186393074E-4 54-55 0.0 0.0 0.0 3.2033844176731554 3.8476781186393074E-4 56-57 0.0 0.0 0.0 3.5854588548540383 3.8476781186393074E-4 58-59 0.0 0.0 0.0 3.9840783079450706 3.8476781186393074E-4 60-61 0.0 0.0 0.0 4.439643397191965 3.8476781186393074E-4 62-63 0.0 0.0 0.0 4.897709477215974 3.8476781186393074E-4 64-65 0.0 0.0 0.0 5.332112336810352 3.8476781186393074E-4 66-67 0.0 0.0 0.0 5.832695260045325 3.8476781186393074E-4 68-69 0.0 0.0 0.0 6.385991373505657 3.8476781186393074E-4 70-71 0.0 0.0 0.0 7.035479439931973 3.8476781186393074E-4 72-73 0.0 0.0 0.0 7.671308249037118 3.8476781186393074E-4 74-75 0.0 0.0 0.0 8.328106903888848 3.8476781186393074E-4 76-77 0.0 0.0 0.0 8.946428777554186 3.8476781186393074E-4 78-79 0.0 0.0 0.0 9.71346341050493 3.8476781186393074E-4 80-81 0.0 0.0 0.0 10.518782440736137 3.8476781186393074E-4 82-83 0.0 0.0 0.0 11.29832202757246 3.8476781186393074E-4 84-85 0.0 0.0 0.0 12.16539629160783 3.8476781186393074E-4 86-87 0.0 0.0 0.0 13.077103621819413 3.8476781186393074E-4 88-89 0.0 0.0 0.0 13.96168482129459 3.8476781186393074E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 20 5.3998217E-4 47.474285 28-29 GTCTTAT 45 9.650643E-4 42.187153 1 GTATGGT 55 0.0025864742 34.516758 1 GTGGTAC 55 0.0025864742 34.516758 1 GGTACAG 55 0.0026159831 34.43703 5 TAAGGGG 55 0.0026159831 34.43703 9 CCTTTAG 60 0.003961439 31.64036 1 CTAGCCA 60 0.004006554 31.567276 4 GGATATG 95 3.500327E-5 29.97508 1 GCTAGAC 40 4.4513287E-4 29.731676 36-37 GAATGCA 65 0.0059261904 29.139025 9 GGGAGCT 65 0.0059261904 29.139025 7 TCTAGGT 65 0.0059261904 29.139025 2 GCCCTGG 65 0.0059261904 29.139025 6 CATGGGT 225 3.6379788E-12 27.358307 4 CGCGCGC 35 0.008330692 27.18062 90-91 CTCGAAC 35 0.008342487 27.172737 46-47 TCGAACT 35 0.008342487 27.172737 48-49 ACTATAT 70 0.008509304 27.057667 8 TATGGGA 70 0.008509304 27.057667 4 >>END_MODULE