FastQCFastQC Report
Fri 27 May 2016
SRR939087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731601
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114630.41964401096646253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75650.2769438142686286No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69100.2529652024581921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60690.2221773970649447No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54950.20116407923411947No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43300.15851509792242718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37850.1385634285534381No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35100.12849607244981973No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29840.10923996586617152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG94800.022.7839181
TACATGG95050.022.3309172
GAGTACT80450.021.66482512-13
GTAAGGT10200.021.3667414
ACATGGG99250.020.8130953
GTACTTT86200.020.08267214-15
AGAGTAC134250.019.84420410-11
AGTACTT83900.019.53203812-13
TAAGGTG13450.019.3740795
GTATAGG6900.019.263461
TAAGGTA7750.018.9513724
GTGTAAG9850.018.3135591
GTATCAA225450.017.5606751
GTACTAG4405.456968E-1217.2620621
CATGGGG61300.017.2355484
TACTTTT96100.016.95416514-15
GGTAAGG13150.016.9337353
ACTTTTT103500.016.93349316-17
GTATTAG7650.016.1338211
GTGTAGG10300.016.130811
Produced by FastQC (version 0.11.5)