##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939087_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731601 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06639878957432 33.0 31.0 34.0 30.0 34.0 2 32.16644524584667 34.0 31.0 34.0 30.0 34.0 3 32.227622555417135 34.0 31.0 34.0 30.0 34.0 4 35.66305291292542 37.0 35.0 37.0 35.0 37.0 5 35.70737527186438 37.0 35.0 37.0 35.0 37.0 6 35.79254437232964 37.0 36.0 37.0 35.0 37.0 7 35.717529756359 37.0 36.0 37.0 35.0 37.0 8 35.70385352765649 37.0 36.0 37.0 35.0 37.0 9 37.38090152990865 39.0 38.0 39.0 35.0 39.0 10-11 37.292604044294904 39.0 37.0 39.0 34.0 39.0 12-13 37.19906311353672 39.0 37.0 39.0 34.0 39.0 14-15 38.504430368856944 40.0 38.0 41.0 34.0 41.0 16-17 38.441927280009054 40.0 38.0 41.0 34.0 41.0 18-19 38.36325638334442 40.0 38.0 41.0 34.0 41.0 20-21 38.25024189111075 40.0 38.0 41.0 34.0 41.0 22-23 38.16846823529498 40.0 38.0 41.0 33.5 41.0 24-25 38.2046461031461 40.0 38.0 41.0 33.5 41.0 26-27 38.117995270905226 40.0 38.0 41.0 33.0 41.0 28-29 37.9964590362941 40.0 38.0 41.0 33.0 41.0 30-31 37.83912419859269 40.0 38.0 41.0 33.0 41.0 32-33 37.635999547518104 40.0 37.5 41.0 32.5 41.0 34-35 37.319263867599986 40.0 37.0 41.0 31.5 41.0 36-37 37.057067814808974 40.0 37.0 41.0 31.0 41.0 38-39 36.80924538393418 40.0 36.5 41.0 30.5 41.0 40-41 36.66846823529498 40.0 36.0 41.0 30.0 41.0 42-43 36.472132277005315 40.0 36.0 41.0 30.0 41.0 44-45 36.20693670122394 39.0 35.5 41.0 29.5 41.0 46-47 35.853203853710696 39.0 35.0 41.0 28.0 41.0 48-49 35.82787676531089 39.0 35.0 41.0 28.0 41.0 50-51 34.81333456094063 38.0 34.0 40.0 25.5 40.5 52-53 34.97349686136445 38.5 34.5 39.5 26.5 40.5 54-55 35.46783809202003 39.0 35.0 41.0 27.0 41.0 56-57 35.32284308725909 39.0 35.0 41.0 26.5 41.0 58-59 35.11341077997848 39.0 35.0 41.0 26.0 41.0 60-61 34.787108366119355 38.0 35.0 40.0 26.0 41.0 62-63 34.41247696863488 37.5 34.0 40.0 25.5 41.0 64-65 34.02570196013254 37.0 34.0 40.0 24.5 41.0 66-67 33.60274194510838 36.5 34.0 39.0 23.0 41.0 68-69 33.185208418066914 36.0 33.0 39.0 22.0 41.0 70-71 32.765059941038245 35.0 33.0 38.5 21.5 40.0 72-73 32.31537951552954 35.0 32.5 37.5 20.0 40.0 74-75 31.87708051066023 35.0 32.0 37.0 18.5 39.0 76-77 31.45755858926688 35.0 32.0 36.5 18.0 39.0 78-79 31.053917098434212 35.0 31.0 36.0 14.5 38.5 80-81 30.64628747024181 35.0 31.0 35.5 9.5 37.0 82-83 30.2362391139848 34.5 31.0 35.0 7.0 37.0 84-85 29.881483606134278 34.0 30.5 35.0 2.0 36.5 86-87 29.533980072492284 34.0 30.0 35.0 2.0 36.0 88-89 29.142255768686567 34.0 29.5 35.0 2.0 36.0 90-91 28.76778947584219 34.0 29.0 35.0 2.0 35.0 92-93 28.36946922336022 34.0 29.0 35.0 2.0 35.0 94-95 27.908283457210622 34.0 28.5 35.0 2.0 35.0 96-97 27.346646710116154 34.0 27.0 35.0 2.0 35.0 98-99 26.647388106828195 33.5 25.5 35.0 2.0 35.0 100-101 24.01766399997657 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10907057806323905 1101 2 0.1654651042007771 1101 3 0.09988960526314372 1101 4 0.11430455231743508 1101 5 0.12933775583209695 1101 6 0.20011871941891712 1101 7 0.11761678592545621 1101 8 0.29019203048950715 1101 9 0.2842549098789533 1101 10-11 0.15905807587338217 1101 12-13 0.2313024993023376 1101 14-15 0.20950661619608724 1101 16-17 0.31142175386248283 1101 18-19 0.33189502005347293 1101 20-21 0.25855221772997083 1101 22-23 0.27022432233074056 1101 24-25 0.3071553031471552 1101 26-27 0.33296701009772534 1101 28-29 0.29064423518501314 1101 30-31 0.2850393108603768 1101 32-33 0.22311692972932917 1101 34-35 0.19885231992777364 1101 36-37 0.060057447276953724 1101 38-39 0.1534949344106522 1101 40-41 -0.08991646136625064 1101 42-43 -0.006758893843432645 1101 44-45 0.013810591638467429 1101 46-47 -0.07636652432179858 1101 48-49 0.12688784427488287 1101 50-51 0.11005634515074547 1101 52-53 0.05210900837791854 1101 54-55 -0.017455637562527215 1101 56-57 0.11102407751086218 1101 58-59 0.12427094491140878 1101 60-61 -0.00609542134784391 1101 62-63 -0.03771458214073675 1101 64-65 -0.091485702573749 1101 66-67 -0.1577713531070728 1101 68-69 -0.07745314315320684 1101 70-71 -0.0806036504422849 1101 72-73 -0.17768975605149961 1101 74-75 -0.19463135282048682 1101 76-77 -0.24001738752064483 1101 78-79 -0.14429349953813642 1101 80-81 -0.2142577698207262 1101 82-83 -0.25691836045315597 1101 84-85 -0.33234158036218986 1101 86-87 -0.24649852398551886 1101 88-89 -0.39796968330444216 1101 90-91 -0.4414999926912522 1101 92-93 -0.45369912945027835 1101 94-95 -0.6134044261984108 1101 96-97 -0.4824426521838845 1101 98-99 -0.6345673361425384 1101 100-101 -0.7110075088449292 1102 1 0.2774957310960744 1102 2 0.35279746368881604 1102 3 0.4451414138329852 1102 4 0.42215374541704875 1102 5 0.35291385488607574 1102 6 0.36551590917963495 1102 7 0.4278672032877253 1102 8 0.4468204389480519 1102 9 0.5056009032011559 1102 10-11 0.4111779284053867 1102 12-13 0.46115433569632813 1102 14-15 0.5682493123563503 1102 16-17 0.6609362224211921 1102 18-19 0.600059669469168 1102 20-21 0.6357871926882339 1102 22-23 0.6606865510452664 1102 24-25 0.6187395407654037 1102 26-27 0.6538166901199887 1102 28-29 0.6809485618405517 1102 30-31 0.7064440827023404 1102 32-33 0.7005936270025614 1102 34-35 0.630639673850979 1102 36-37 0.7025271885123416 1102 38-39 0.7372477168257845 1102 40-41 0.5851012765302386 1102 42-43 0.689716133980717 1102 44-45 0.5992199544654042 1102 46-47 0.5370701061734735 1102 48-49 0.5650232783316511 1102 50-51 0.6271956745886982 1102 52-53 0.6357470161686791 1102 54-55 0.717990592264961 1102 56-57 0.6850477975998928 1102 58-59 0.6207014540933855 1102 60-61 0.5494433237829313 1102 62-63 0.6616099865849208 1102 64-65 0.7725345394963696 1102 66-67 0.7227117437209714 1102 68-69 0.6819100315824613 1102 70-71 0.6261468364803591 1102 72-73 0.664766768711992 1102 74-75 0.5475434524104514 1102 76-77 0.6427142485394555 1102 78-79 0.6881192021869609 1102 80-81 0.5106107610640827 1102 82-83 0.42852230454406026 1102 84-85 0.4119301224758729 1102 86-87 0.1096582651074165 1102 88-89 0.15264696945014933 1102 90-91 0.04168204681903731 1102 92-93 0.1514278182403146 1102 94-95 0.1444266812028161 1102 96-97 0.1302413350170042 1102 98-99 -0.06172301226941812 1102 100-101 -0.12199213600130321 1103 1 0.19854751103134305 1103 2 0.1498322533328249 1103 3 0.2954731290433372 1103 4 0.1862799590092763 1103 5 0.17588715585694814 1103 6 0.21958144316021588 1103 7 0.21123289908060627 1103 8 0.11270636127814981 1103 9 0.22049167990018503 1103 10-11 0.2511556792144418 1103 12-13 0.23767132598759133 1103 14-15 0.3068637945569961 1103 16-17 0.38863039717847414 1103 18-19 0.3647866112167364 1103 20-21 0.4085986813613687 1103 22-23 0.3825130073559464 1103 24-25 0.4055538611537486 1103 26-27 0.33427987782225443 1103 28-29 0.32720462980172016 1103 30-31 0.33926972995153193 1103 32-33 0.3743861027307176 1103 34-35 0.30807697158560643 1103 36-37 0.18449927589098536 1103 38-39 0.2643876844633297 1103 40-41 0.24374407588299363 1103 42-43 0.16142932168298074 1103 44-45 0.19247844961103766 1103 46-47 0.096655462160534 1103 48-49 0.04475831069410674 1103 50-51 0.06699543187024659 1103 52-53 0.19102209707482842 1103 54-55 0.15359941103518793 1103 56-57 0.03577393028598408 1103 58-59 -0.043313513544156024 1103 60-61 0.07564680274732893 1103 62-63 0.028507235775855122 1103 64-65 -0.03168673882078821 1103 66-67 -0.13085824009780112 1103 68-69 -0.20894150563436398 1103 70-71 -0.0032079369824202786 1103 72-73 -0.10790111154690862 1103 74-75 -0.21296827898113335 1103 76-77 -0.32514578382300385 1103 78-79 -0.22377602435025423 1103 80-81 -0.28363276644401125 1103 82-83 -0.15878149804493802 1103 84-85 -0.1971111396600449 1103 86-87 -0.18490888699614416 1103 88-89 -0.15318029592201654 1103 90-91 -0.155051367420441 1103 92-93 -0.17668100052991065 1103 94-95 -0.4121105356256649 1103 96-97 -0.29215550964318737 1103 98-99 -0.5165652452791285 1103 100-101 -0.6798177832504919 1104 1 0.37524024207411344 1104 2 0.3078947690780396 1104 3 0.3178410146313908 1104 4 0.2367944639799191 1104 5 0.3588295235487493 1104 6 0.35330283532733375 1104 7 0.2411206963016994 1104 8 0.32951255471651564 1104 9 0.4305734580913736 1104 10-11 0.3506302737147635 1104 12-13 0.3242905632412345 1104 14-15 0.45053349399308473 1104 16-17 0.4496438072515261 1104 18-19 0.538419200407283 1104 20-21 0.4852158354028049 1104 22-23 0.5668230280911786 1104 24-25 0.5950232234001334 1104 26-27 0.5296013707587122 1104 28-29 0.560266176611016 1104 30-31 0.5652277653370703 1104 32-33 0.5441153335893887 1104 34-35 0.5023524982022778 1104 36-37 0.7121117693506633 1104 38-39 0.7300146828936462 1104 40-41 0.6741693903026942 1104 42-43 0.5950741679128484 1104 44-45 0.5350769903935415 1104 46-47 0.4670987987882427 1104 48-49 0.6054037673536001 1104 50-51 0.6384643871635447 1104 52-53 0.5960913275459276 1104 54-55 0.7292243747000882 1104 56-57 0.5263040351248449 1104 58-59 0.5268379311392906 1104 60-61 0.6386586431442041 1104 62-63 0.6121164736108682 1104 64-65 0.6169170245263089 1104 66-67 0.5862783604874338 1104 68-69 0.49743772619324034 1104 70-71 0.457413054045233 1104 72-73 0.5348977567359441 1104 74-75 0.5028665562028678 1104 76-77 0.4656808154057259 1104 78-79 0.46126166725682083 1104 80-81 0.40890666924771324 1104 82-83 0.5706542905720049 1104 84-85 0.5563787252702852 1104 86-87 0.44737533097567805 1104 88-89 0.46039522294316626 1104 90-91 0.410538084743191 1104 92-93 0.3937269699369246 1104 94-95 0.26210632192137595 1104 96-97 0.180644279128785 1104 98-99 0.22542020130343587 1104 100-101 0.13164933106456544 1105 1 0.08752223871701403 1105 2 0.11428437555097304 1105 3 0.20107415074484436 1105 4 0.26576872222288017 1105 5 0.23285007092318466 1105 6 0.23177253608486836 1105 7 0.07303618937122991 1105 8 0.14596473980954983 1105 9 0.2133220761633794 1105 10-11 0.18912068851141584 1105 12-13 0.2055753098513975 1105 14-15 0.20999508770426445 1105 16-17 0.25290607000237486 1105 18-19 0.3484282082365624 1105 20-21 0.28099842529260854 1105 22-23 0.2122732806675387 1105 24-25 0.21952689994367347 1105 26-27 0.08850066957658242 1105 28-29 0.17145096409370097 1105 30-31 0.18237557706947172 1105 32-33 0.27270388213840846 1105 34-35 0.21882928617702646 1105 36-37 0.281315707399024 1105 38-39 0.3122220375679987 1105 40-41 0.1507958590352061 1105 42-43 0.11876475889787486 1105 44-45 0.15744021622816007 1105 46-47 0.17979666349620516 1105 48-49 0.16674668787439373 1105 50-51 0.13629814311089916 1105 52-53 0.006384855797577416 1105 54-55 0.1823896147964419 1105 56-57 0.07669748282189914 1105 58-59 0.27507148391445213 1105 60-61 0.26153535492938573 1105 62-63 0.26736462541857975 1105 64-65 0.2462559609675381 1105 66-67 0.1749296860668963 1105 68-69 0.09482545570439527 1105 70-71 -0.07713058234004677 1105 72-73 -0.06217450034036176 1105 74-75 0.09017226288096225 1105 76-77 0.10269188273428753 1105 78-79 0.05675695897954114 1105 80-81 -0.16615116736533864 1105 82-83 -0.16717144959775965 1105 84-85 -0.10326619959172234 1105 86-87 -0.19218649963744738 1105 88-89 -0.22236172836297285 1105 90-91 -0.11130213144470957 1105 92-93 -0.17574125067949709 1105 94-95 -0.20559485623920182 1105 96-97 -0.13169247876695778 1105 98-99 -0.2618696325477501 1105 100-101 -0.21043191399997596 1106 1 0.24698271326870014 1106 2 0.23965955808409234 1106 3 0.27733103804742854 1106 4 0.30073460985208555 1106 5 0.3261707164995187 1106 6 0.3127111445265598 1106 7 0.31480833449203516 1106 8 0.30994583974322865 1106 9 0.40331685164286313 1106 10-11 0.4735821383579264 1106 12-13 0.4259268443534978 1106 14-15 0.5693990334029095 1106 16-17 0.527221011442613 1106 18-19 0.5208443462679284 1106 20-21 0.5663994474015794 1106 22-23 0.5909436210722063 1106 24-25 0.5422303866035492 1106 26-27 0.551124388593756 1106 28-29 0.5840520792190347 1106 30-31 0.6688797149968124 1106 32-33 0.5597990104833812 1106 34-35 0.5317975711191565 1106 36-37 0.498472023064096 1106 38-39 0.8392736562896843 1106 40-41 0.8141949183155432 1106 42-43 0.6510069349297964 1106 44-45 0.7809750365482486 1106 46-47 0.7127664477150972 1106 48-49 0.7362648616329182 1106 50-51 0.7352276901074859 1106 52-53 0.6836439637126048 1106 54-55 0.6626524012940749 1106 56-57 0.7345193090786708 1106 58-59 0.7354140839510208 1106 60-61 0.7866110645806756 1106 62-63 0.8146257298194968 1106 64-65 0.6515079427283794 1106 66-67 0.6704668899310491 1106 68-69 0.7762154535396206 1106 70-71 0.7279609156133588 1106 72-73 0.7025833167432864 1106 74-75 0.5142533247972665 1106 76-77 0.608996953425951 1106 78-79 0.6218635108954409 1106 80-81 0.5838807687593466 1106 82-83 0.6010743277581163 1106 84-85 0.568550457220006 1106 86-87 0.4350701222319522 1106 88-89 0.5074682949599598 1106 90-91 0.6515444137550439 1106 92-93 0.6011151178427276 1106 94-95 0.47983485101171297 1106 96-97 0.5989000338755446 1106 98-99 0.803365964260017 1106 100-101 0.5946186598175984 1107 1 0.148948273044482 1107 2 0.1807711050612184 1107 3 0.14842027684325387 1107 4 0.19309163672684093 1107 5 0.16004602368146692 1107 6 0.16814707622195613 1107 7 0.126244363317781 1107 8 0.17369841290247479 1107 9 0.258642789001307 1107 10-11 0.20538732610135213 1107 12-13 0.23603694275868747 1107 14-15 0.3198876481940687 1107 16-17 0.3726573594109226 1107 18-19 0.327510364435355 1107 20-21 0.23730954430355666 1107 22-23 0.20759160363730444 1107 24-25 0.30766030835869174 1107 26-27 0.37076149978690154 1107 28-29 0.21572708049821188 1107 30-31 0.18425102610328992 1107 32-33 0.2715883364941689 1107 34-35 0.3393525776826891 1107 36-37 0.41483607280986945 1107 38-39 0.5721440979928332 1107 40-41 0.3434347663874959 1107 42-43 0.3034080636075629 1107 44-45 0.47993918186335094 1107 46-47 0.5612128246808652 1107 48-49 0.689684717099496 1107 50-51 0.5538718892142924 1107 52-53 0.4691360720164184 1107 54-55 0.5874347758362077 1107 56-57 0.6019841549752982 1107 58-59 0.5839100378649817 1107 60-61 0.5586054512560068 1107 62-63 0.5102573068954612 1107 64-65 0.5234206213896826 1107 66-67 0.3495923785819812 1107 68-69 0.5502187906608498 1107 70-71 0.5788169779779793 1107 72-73 0.6372281073045301 1107 74-75 0.6491323266904416 1107 76-77 0.7108538052637243 1107 78-79 0.6561637488936967 1107 80-81 0.6628149308612272 1107 82-83 0.5897496085761134 1107 84-85 0.6550790706351535 1107 86-87 0.5726688461241913 1107 88-89 0.5970219694501502 1107 90-91 0.5071677405330384 1107 92-93 0.4057151648468782 1107 94-95 0.3016386771958004 1107 96-97 0.35710068262532246 1107 98-99 0.3841982875051855 1107 100-101 0.41008697118627424 1108 1 0.03664186004802161 1108 2 0.1266806476569471 1108 3 0.09862224529925356 1108 4 0.06728568467519835 1108 5 0.1297395332530158 1108 6 0.15557223230634065 1108 7 0.22193740493074898 1108 8 0.1981557015818609 1108 9 0.24905777400025642 1108 10-11 0.29840798192791596 1108 12-13 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-1.3731057686142876 2316 52-53 -1.3216138090787979 2316 54-55 -1.1601117428325622 2316 56-57 -1.153184033724223 2316 58-59 -1.0693568008316419 2316 60-61 -0.9211973578793291 2316 62-63 -1.150706366448503 2316 64-65 -1.2028261999920176 2316 66-67 -1.2838938502219932 2316 68-69 -1.1973460083077683 2316 70-71 -1.2385590710958425 2316 72-73 -1.0572371149496718 2316 74-75 -1.0904244859410142 2316 76-77 -1.0342539225866823 2316 78-79 -0.8214519411042289 2316 80-81 -0.7182715529612054 2316 82-83 -0.9306993091969957 2316 84-85 -0.9716500745250087 2316 86-87 -0.8346949888375015 2316 88-89 -0.9681794418994549 2316 90-91 -1.1333495002749494 2316 92-93 -1.256977736498488 2316 94-95 -1.0220613615297154 2316 96-97 -0.9909859388240179 2316 98-99 -1.0452179402317832 2316 100-101 -0.8045035909655311 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9269.0 3 2341.0 4 2469.0 5 2774.0 6 3089.0 7 3785.0 8 4593.0 9 5662.0 10 6746.0 11 8382.0 12 10585.0 13 15555.0 14 13730.0 15 13904.0 16 16513.0 17 21395.0 18 20116.0 19 15006.0 20 15153.0 21 15817.0 22 17177.0 23 18739.0 24 20755.0 25 23742.0 26 27435.0 27 31850.0 28 37505.0 29 44838.0 30 54028.0 31 66358.0 32 83319.0 33 108348.0 34 152764.0 35 229248.0 36 379457.0 37 600386.0 38 537154.0 39 91567.0 40 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.749063334734046 18.30111694578067 14.577890879598476 23.371928839886806 2 15.414737988745525 19.86898979121714 39.79431426542982 24.921957954607514 3 19.876965140123033 23.64384028557868 29.26136936296383 27.21782521133445 4 13.425461486837412 16.1459457735785 36.32322826550297 34.10536447408112 5 14.462517097004673 36.6528253178274 33.57970860948517 15.304948975682752 6 32.74490757924566 34.87098614503941 17.1201262786242 15.263979997090726 7 29.102822347927 32.06221836891515 20.412617365752414 18.42234191740544 8 26.380690271843093 34.097692008883094 19.601250375625735 19.920367343648078 9 26.286975597726897 17.547490391630156 20.441708367975007 35.72382564266794 10-11 25.63601614553257 25.77122179002014 26.58153962954386 22.01122243490343 12-13 26.783578224315484 23.172459937381635 27.110081212602893 22.933880625699988 14-15 23.28354889206136 24.683788977190446 25.846820411046846 26.185841719701347 16-17 22.704225899118992 27.8248702197192 26.405471390448444 23.065432490713363 18-19 22.79368020238069 26.775626883416916 28.170686058001408 22.260006856200988 20-21 23.62063152339627 25.55333426175677 28.033800721238418 22.79223349360854 22-23 23.16952914871755 25.400207188711605 28.31021350919512 23.120050153375722 24-25 23.444103678095484 25.59558709920064 28.067909786706497 22.892399435997376 26-27 22.9316221567577 25.646919356142384 28.563039458392158 22.85841902870776 28-29 22.69747090911357 26.589836278771074 28.114354143100844 22.598338669014513 30-31 22.890132480501027 25.760744913737593 27.89843931248092 23.450683293280463 32-33 22.848788661224788 25.873282967851 27.987273581173433 23.29065478975077 34-35 23.702351767328622 25.858550167406197 27.628752911541838 22.81034515372335 36-37 23.489848567762824 25.433957058177022 28.148994973718942 22.927199400341216 38-39 23.02621718713939 25.89255486318569 28.071138474892166 23.01008947478275 40-41 23.152091498028707 26.049867366982728 27.797243939586707 23.00079719540186 42-43 23.042084970347076 26.062276471988167 27.207140831429488 23.68849772623527 44-45 23.202483344252624 25.821806770155288 27.563583552424177 23.41212633316792 46-47 23.784241954682333 26.258663095200653 27.125758977916174 22.83133597220084 48-49 23.533312900974316 26.12063088158516 27.097704818148937 23.24835139929159 50-51 22.962389883520988 26.57748087194078 27.41446911956113 23.0456601249771 52-53 23.51514480375641 26.63742349051387 26.66623009152994 23.18120161419978 54-55 23.010880050910288 26.20741344285421 27.04430648067 23.7374000255655 56-57 23.29545684400837 26.30135842692125 26.926142133006458 23.477042596063917 58-59 23.690834272628717 25.969392360610005 26.894850332603408 23.44492303415787 60-61 23.158233650872763 26.147172972890527 27.658898813182837 23.035694563053877 62-63 22.830054223243078 26.405772288125227 27.490402660681294 23.273770827950393 64-65 22.710360044312466 26.67557895209331 27.15238683562113 23.461674167973097 66-67 22.873738438561418 26.32418647247755 26.936859468614443 23.865215620346586 68-69 22.835698083323397 26.126310622018302 27.355973256907674 23.682018037750623 70-71 23.22248809901852 26.22925906478174 26.9159595868905 23.63229324930923 72-73 23.131777218427743 26.05123118126657 27.299999963206034 23.51699163709965 74-75 22.81894839408103 27.018170953115835 26.767732520273153 23.39514813252999 76-77 23.06446335048439 26.364875943444726 26.71345822650325 23.857202479567626 78-79 23.127011989410992 26.416739384097916 26.96958189239414 23.48666673409695 80-81 23.4048547674499 26.445980818092607 26.737725889101565 23.411438525355926 82-83 23.213399572181892 26.36724303783965 27.022176280925308 23.397181109053154 84-85 23.10158362302047 26.612334234582207 26.82929610623701 23.456786036160313 86-87 23.130646099356266 26.631250692053555 26.731991617001288 23.506111591588898 88-89 22.95519817042885 26.60760761350015 26.348135072151948 24.08905914391905 90-91 23.118685131038706 26.321223847270787 26.600643804777313 23.959447216913198 92-93 23.444873792137663 26.326873249054938 26.759610098983817 23.46864285982358 94-95 23.147429438126714 26.134307881636094 26.9514867975848 23.766775882652393 96-97 22.955121769394214 26.018633045571466 27.3031390347384 23.723106150295923 98-99 23.03473333701271 26.473204923008996 26.43424030023732 24.057821439740973 100-101 23.198077846693852 25.53511945306483 27.073287245261252 24.193515454980066 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2545.0 1 1453.0 2 397.0 3 429.0 4 400.0 5 399.0 6 633.5 7 1296.5 8 2167.0 9 2610.0 10 2517.0 11 2497.0 12 2665.5 13 2879.5 14 3157.5 15 3459.5 16 3754.0 17 3935.0 18 4104.5 19 4253.5 20 4483.5 21 5037.5 22 5835.0 23 7122.5 24 8796.5 25 10698.0 26 13561.0 27 16916.5 28 19924.5 29 23282.5 30 27049.5 31 31647.0 32 37222.5 33 39860.5 34 41350.0 35 43547.5 36 47154.0 37 52342.5 38 56638.5 39 59916.5 40 63440.5 41 69762.5 42 74858.0 43 78803.5 44 83975.0 45 90645.0 46 96673.5 47 107836.0 48 127659.5 49 126628.0 50 109130.0 51 102167.5 52 102250.5 53 104495.0 54 104808.0 55 99024.0 56 90621.0 57 83530.0 58 77416.5 59 68872.0 60 57277.0 61 47115.0 62 38618.0 63 29921.0 64 22287.0 65 17158.0 66 13462.0 67 11034.0 68 8860.0 69 6794.5 70 5098.5 71 3576.0 72 2677.0 73 2230.0 74 1763.5 75 1438.5 76 1057.0 77 640.5 78 405.5 79 271.0 80 166.5 81 118.5 82 84.5 83 51.5 84 45.0 85 34.0 86 25.0 87 24.5 88 18.5 89 14.0 90 14.5 91 12.5 92 7.0 93 7.0 94 6.5 95 5.5 96 6.0 97 5.5 98 4.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33595682531965687 2 0.1194903648080375 3 0.11370621111941312 4 0.12080827324342025 5 0.13852681998578856 6 0.08778734522355204 7 0.09635375005354004 8 0.10327276933929956 9 0.09042316209431758 10-11 0.08877577655008913 12-13 0.0926013718694641 14-15 0.09404741029162018 16-17 0.08333940425413522 18-19 0.09827569985513988 20-21 0.10839796881023255 22-23 0.11606746373280724 24-25 0.1317725392544519 26-27 0.13089393363086335 28-29 0.12545756133490946 30-31 0.1143651653371045 32-33 0.10786714457931448 34-35 0.3306302787266515 36-37 0.49013380797561573 38-39 0.4641417249444556 40-41 0.4417555858267734 42-43 0.4752524252260854 44-45 0.4821348359441953 46-47 0.49017041654326526 48-49 0.5011712911219465 50-51 0.4884681181475625 52-53 0.49326384050964983 54-55 0.479334280519007 56-57 0.4576070956190161 58-59 0.4363924306661185 60-61 0.39670874333403744 62-63 0.4667043246799222 64-65 0.4989747770629752 66-67 0.5065893591340755 68-69 0.5194389663790575 70-71 0.5113484729285134 72-73 0.5038803251280111 74-75 0.5113118643608638 76-77 0.487058688293056 78-79 0.4665212818416745 80-81 0.4681869716697278 82-83 0.48162231599710204 84-85 0.4814392731588545 86-87 0.48477065281496096 88-89 0.49740060865404573 90-91 0.5127762070668447 92-93 0.504575887913352 94-95 0.508767568909222 96-97 0.5089506117474697 98-99 0.5034044137485673 100-101 0.4905365022197605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731601.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.11436705785157 #Duplication Level Percentage of deduplicated Percentage of total 1 71.89704927608537 35.31178068522098 2 14.376442965859205 14.121797936229544 3 5.494956706006615 8.096439618774356 4 2.6613612336164194 5.228442900054939 5 1.5059601360172445 3.698213944742151 6 0.9208742742255043 2.7136894271066447 7 0.6283168430636735 2.160156884120185 8 0.4299514694207591 1.6893435428955046 9 0.3150468833888689 1.3925995444073762 >10 1.6323516856664222 14.148018457668496 >50 0.0893569682477952 2.9975515563953152 >100 0.0420621652268182 3.695860549365188 >500 0.003582510385917963 1.259418322380545 >1k 0.0023137046242117324 2.1119826132473896 >5k 2.9854253215635257E-4 0.954506903867895 >10k+ 7.463563303908814E-5 0.4201971135234717 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11463 0.41964401096646253 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7565 0.2769438142686286 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6910 0.2529652024581921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6069 0.2221773970649447 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5495 0.20116407923411947 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4330 0.15851509792242718 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3785 0.1385634285534381 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3510 0.12849607244981973 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2984 0.10923996586617152 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.321713529904257E-5 2 0.0 0.0 0.0 0.0 7.321713529904257E-5 3 0.0 0.0 0.0 0.0 7.321713529904257E-5 4 0.0 0.0 0.0 0.0 7.321713529904257E-5 5 0.0 0.0 0.0 0.0 1.4643427059808515E-4 6 0.0 0.0 0.0 0.0 1.4643427059808515E-4 7 0.0 0.0 0.0 0.0 1.4643427059808515E-4 8 0.0 0.0 0.0 0.0 1.4643427059808515E-4 9 0.0 0.0 0.0 0.0 1.4643427059808515E-4 10-11 0.0 0.0 0.0 0.0 1.4643427059808515E-4 12-13 0.0 0.0 0.0 0.0 4.942156632685374E-4 14-15 0.0 0.0 0.0 0.0 5.12519947093298E-4 16-17 0.0 0.0 0.0 3.6608567649521286E-5 5.308242309180586E-4 18-19 0.0 0.0 0.0 1.4643427059808515E-4 5.857370823923406E-4 20-21 0.0 0.0 0.0 2.3795568972188836E-4 7.138670691656651E-4 22-23 0.0 0.0 0.0 3.477813926704522E-4 7.504756368151863E-4 24-25 0.0 0.0 0.0 4.0269424414473415E-4 7.68779920639947E-4 26-27 0.0 0.0 0.0 7.68779920639947E-4 8.053884882894683E-4 28-29 0.0 0.0 0.0 0.0026358168707655326 8.419970559389896E-4 30-31 0.0 0.0 0.0 0.00823692772114229 8.603013397637503E-4 32-33 0.0 0.0 0.0 0.01760872103941974 9.518227588875534E-4 34-35 0.0 0.0 0.0 0.033679882237559584 0.001079952745660878 36-37 0.0 0.0 0.0 0.058042884008316004 0.0010982570294856386 38-39 0.0 0.0 0.0 0.09628053291824099 0.00113486559713516 40-41 0.0 0.0 0.0 0.1401375969623675 0.00113486559713516 42-43 0.0 0.0 0.0 0.18659386930961003 0.0011531698809599206 44-45 0.0 0.0 0.0 0.24472827473704978 0.0011714741647846812 46-47 0.0 0.0 0.0 0.3161149816536163 0.0012080827324342023 48-49 0.0 0.0 0.0 0.38993615831887596 0.0012996041515580057 50-51 0.0 0.0 0.0 0.4709509185272666 0.0013545170030322877 52-53 0.0 0.0 0.0 0.5525331115342248 0.0014826469898056123 54-55 0.0 0.0 0.0 0.653005325448336 0.0016107769765789366 56-57 0.0 0.0 0.0 0.7727153416622705 0.0016290812604036974 58-59 0.0 0.0 0.0 0.8995640285678619 0.001647385544228458 60-61 0.0 0.0 0.0 1.0418249224539016 0.001647385544228458 62-63 0.0 0.0 0.0 1.188295801619636 0.001647385544228458 64-65 0.0 0.0 0.0 1.351515100485027 0.001647385544228458 66-67 0.0 0.0 0.0 1.545522204743665 0.001647385544228458 68-69 0.0 0.0 0.0 1.763105226568595 0.001647385544228458 70-71 0.0 0.0 0.0 1.9961919767930967 0.0017022983957027398 72-73 0.0 0.0 0.0 2.2352642278282957 0.0017938198148265431 74-75 0.0 0.0 0.0 2.4993218262842927 0.0018853412339503463 76-77 0.0 0.0 0.0 2.7825074013371642 0.001903645517775107 78-79 0.0 0.0 0.0 3.1187753994818426 0.002068384072197953 80-81 0.0 0.0 0.0 3.474482547048416 0.002104992639847474 82-83 0.0 0.0 0.0 3.839323532243545 0.0022331226266207984 84-85 0.0 0.0 0.0 4.2274109578961205 0.00226973119427032 86-87 0.0 0.0 0.0 4.637939435517852 0.0023429483295693623 88-89 0.0 0.0 0.0 5.089158336082027 0.002416165464868405 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9480 0.0 22.783918 1 TACATGG 9505 0.0 22.330917 2 GAGTACT 8045 0.0 21.664825 12-13 GTAAGGT 1020 0.0 21.366741 4 ACATGGG 9925 0.0 20.813095 3 GTACTTT 8620 0.0 20.082672 14-15 AGAGTAC 13425 0.0 19.844204 10-11 AGTACTT 8390 0.0 19.532038 12-13 TAAGGTG 1345 0.0 19.374079 5 GTATAGG 690 0.0 19.26346 1 TAAGGTA 775 0.0 18.951372 4 GTGTAAG 985 0.0 18.313559 1 GTATCAA 22545 0.0 17.560675 1 GTACTAG 440 5.456968E-12 17.262062 1 CATGGGG 6130 0.0 17.235548 4 TACTTTT 9610 0.0 16.954165 14-15 GGTAAGG 1315 0.0 16.933735 3 ACTTTTT 10350 0.0 16.933493 16-17 GTATTAG 765 0.0 16.133821 1 GTGTAGG 1030 0.0 16.13081 1 >>END_MODULE