FastQCFastQC Report
Fri 27 May 2016
SRR939087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731601
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129550.47426399389954826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80570.294955229552193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65700.24051828945735484No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62320.22814459359181669No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48660.1781372901825706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42850.15686771237819871No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40790.14932634744239734No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37050.13563474314147636No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31480.11524377096069302No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29920.10953283440736769No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29590.1083247516749335No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27870.10202807803921583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97800.021.9585441
TACATGG97900.021.32472
TAAGGTG14700.021.302945
GAGTACT86200.020.7532812-13
GTAAGGT13650.020.5084254
ACATGGG99600.020.341433
CGTTAGG1404.890451E-420.3346252
GTACTTT88900.019.93616114-15
AGAGTAC143850.019.21457710-11
AGTACTT88700.019.17865812-13
GTAGGAC4800.018.7812863
GGTAAGG15700.017.2261473
TACTTTT99700.017.01506414-15
CATGGGG62850.016.7594834
ACTTTTT108350.016.48803316-17
AGGTAAG15350.016.382512
ATACAGT17550.016.2213526
TATAGCG2053.3623708E-416.201575
TTACGGT2355.1874267E-516.1523274
ACCGTCC3302.306515E-715.8158198
Produced by FastQC (version 0.11.5)