##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939087_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731601 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53821183986973 34.0 31.0 34.0 31.0 34.0 2 32.74893880914526 34.0 31.0 34.0 31.0 34.0 3 32.83867116756803 34.0 31.0 34.0 31.0 34.0 4 36.27973082452379 37.0 37.0 37.0 35.0 37.0 5 36.24029278068063 37.0 37.0 37.0 35.0 37.0 6 36.27762619796962 37.0 37.0 37.0 35.0 37.0 7 36.23174541230583 37.0 37.0 37.0 35.0 37.0 8 36.216944568405125 37.0 37.0 37.0 35.0 37.0 9 37.97219689112722 39.0 38.0 39.0 35.0 39.0 10-11 37.9152240755513 39.0 38.0 39.0 35.0 39.0 12-13 37.85354834033228 39.0 38.0 39.0 35.0 39.0 14-15 39.23749606915505 41.0 39.0 41.0 36.0 41.0 16-17 39.187012488280686 40.0 39.0 41.0 36.0 41.0 18-19 39.21102386475916 40.5 38.5 41.0 36.0 41.0 20-21 39.265251769932725 41.0 39.0 41.0 36.0 41.0 22-23 39.228279679206445 41.0 39.0 41.0 36.0 41.0 24-25 39.16813088734409 41.0 39.0 41.0 36.0 41.0 26-27 39.035718613369966 40.5 39.0 41.0 35.0 41.0 28-29 38.89502383400797 40.0 38.0 41.0 35.0 41.0 30-31 38.71864027725864 40.0 38.0 41.0 35.0 41.0 32-33 38.57962162116649 40.0 38.0 41.0 34.5 41.0 34-35 38.519424872080506 40.0 38.0 41.0 34.0 41.0 36-37 38.413784626671315 40.0 38.0 41.0 34.0 41.0 38-39 38.2800328452069 40.0 38.0 41.0 34.0 41.0 40-41 38.125536086712515 40.0 38.0 41.0 33.5 41.0 42-43 37.96484021641521 40.0 38.0 41.0 33.0 41.0 44-45 37.748773887548 40.0 37.0 41.0 33.0 41.0 46-47 37.47420121020603 40.0 37.0 41.0 33.0 41.0 48-49 37.159623422308016 40.0 37.0 41.0 32.0 41.0 50-51 36.89906358944809 40.0 36.0 41.0 31.0 41.0 52-53 36.66965197332993 39.5 36.0 41.0 31.0 41.0 54-55 36.41596228731795 39.0 35.0 41.0 31.0 41.0 56-57 36.11495108546234 39.0 35.0 40.5 30.0 41.0 58-59 35.80227053658275 38.5 35.0 40.0 29.5 41.0 60-61 35.411086575235544 38.0 35.0 40.0 28.5 41.0 62-63 35.016527486993894 37.5 34.0 40.0 28.0 41.0 64-65 34.60140829498891 37.0 34.0 39.5 28.0 41.0 66-67 34.21416341552079 36.0 34.0 39.0 27.0 41.0 68-69 33.74032829099126 36.0 33.5 39.0 26.0 40.0 70-71 33.28899022953938 35.0 33.0 38.0 26.0 40.0 72-73 32.82858678848045 35.0 33.0 37.0 25.5 39.5 74-75 32.359425479782736 35.0 32.0 37.0 24.5 39.0 76-77 30.412061644434893 33.5 29.5 35.0 20.5 37.0 78-79 31.386347420432195 34.5 31.0 36.0 22.0 37.5 80-81 31.426400854297533 35.0 32.0 35.5 23.0 37.0 82-83 31.155989655883126 35.0 31.5 35.0 21.0 37.0 84-85 30.81049831216199 34.0 31.0 35.0 20.0 36.0 86-87 30.306764421304578 34.0 31.0 35.0 17.5 36.0 88-89 29.78176735914213 34.0 30.0 35.0 8.5 35.5 90-91 29.353180058141724 34.0 30.0 35.0 4.5 35.0 92-93 29.100064394470493 34.0 29.0 35.0 2.0 35.0 94-95 28.792440953126025 34.0 29.0 35.0 2.0 35.0 96-97 28.261945833231135 34.0 29.0 35.0 2.0 35.0 98-99 27.51804436299445 34.0 28.5 35.0 2.0 35.0 100-101 23.877945205028112 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09611406050595406 1101 2 0.09148166618592057 1101 3 0.13966971444208554 1101 4 0.09207006619667624 1101 5 0.127724693848684 1101 6 0.12226175511028714 1101 7 0.11380514036320477 1101 8 0.10246930700110113 1101 9 0.15802736160313202 1101 10-11 0.12279823671945422 1101 12-13 0.09195788116581838 1101 14-15 0.13952248574940995 1101 16-17 0.14577569898835918 1101 18-19 0.22505948799182107 1101 20-21 0.2282295180254792 1101 22-23 0.168709193127917 1101 24-25 0.14986922623722876 1101 26-27 0.18330852939405418 1101 28-29 0.09488438861747284 1101 30-31 0.034452097173641505 1101 32-33 0.05315315693524525 1101 34-35 0.19660125929721772 1101 36-37 0.25719194289353453 1101 38-39 0.3634303864724373 1101 40-41 0.3234441587122987 1101 42-43 0.35392694163875404 1101 44-45 0.4730550256658148 1101 46-47 0.3948926414655318 1101 48-49 0.3639609833373356 1101 50-51 0.43996640156355227 1101 52-53 0.42458595195643767 1101 54-55 0.3744186945198962 1101 56-57 0.3376554201622284 1101 58-59 0.44064006773513853 1101 60-61 0.44070412449922713 1101 62-63 0.4649067088639569 1101 64-65 0.43273112344714093 1101 66-67 0.36985527059365353 1101 68-69 0.24235677488800889 1101 70-71 0.28616877539015917 1101 72-73 0.26393547831508357 1101 74-75 0.30848479347127267 1101 76-77 0.28569808173849864 1101 78-79 0.4208676046618969 1101 80-81 0.3328769918996777 1101 82-83 0.47080737845467624 1101 84-85 0.4525228272562387 1101 86-87 0.49352978846936324 1101 88-89 0.6018579086308549 1101 90-91 0.5754088249499283 1101 92-93 0.501055237788087 1101 94-95 0.5232226014615939 1101 96-97 0.47058894638078286 1101 98-99 0.5399720414292553 1101 100-101 0.12668609055004865 1102 1 0.24086642555882065 1102 2 0.12922999673683933 1102 3 0.2384315397064185 1102 4 0.13227526931297717 1102 5 0.14417781015364284 1102 6 0.17616687475413784 1102 7 0.2140451236687113 1102 8 0.1444211712192427 1102 9 0.2586207116309538 1102 10-11 0.20792170639112584 1102 12-13 0.24202639813854887 1102 14-15 0.2867804156325491 1102 16-17 0.3128979861335992 1102 18-19 0.23555545348986584 1102 20-21 0.27716593983961246 1102 22-23 0.2574049360327635 1102 24-25 0.2732804671943825 1102 26-27 0.2891853379638931 1102 28-29 0.12406044593522125 1102 30-31 0.16130831587147298 1102 32-33 0.23291247802595905 1102 34-35 0.22184367721596487 1102 36-37 0.29687509536989154 1102 38-39 0.3392132885314183 1102 40-41 0.3349595315559242 1102 42-43 0.34941177747625574 1102 44-45 0.318272054046453 1102 46-47 0.33508462810993933 1102 48-49 0.2865900178392877 1102 50-51 0.36602684953239617 1102 52-53 0.41604011166705845 1102 54-55 0.4705511374803919 1102 56-57 0.5356127796502577 1102 58-59 0.49622889633836564 1102 60-61 0.4279801400807557 1102 62-63 0.38676118855233454 1102 64-65 0.34720489918448294 1102 66-67 0.4902924575719467 1102 68-69 0.4512984137305267 1102 70-71 0.45121468523991126 1102 72-73 0.4649781883874269 1102 74-75 0.5262675564095005 1102 76-77 0.5365571524118415 1102 78-79 0.44552328078878034 1102 80-81 0.40886787949010284 1102 82-83 0.284512512010604 1102 84-85 0.4243366141232414 1102 86-87 0.6125660294265138 1102 88-89 0.5928387099664114 1102 90-91 0.8165183129855471 1102 92-93 0.6247892389010516 1102 94-95 0.5313910766981031 1102 96-97 0.4553527888960822 1102 98-99 0.3768849518354891 1102 100-101 0.1001602836496609 1103 1 0.04745218607661883 1103 2 0.030124009681820496 1103 3 0.10954804456078904 1103 4 0.08897834374663915 1103 5 0.09587279397241133 1103 6 0.14899049934960829 1103 7 0.11175952496321173 1103 8 0.11946162429367746 1103 9 0.15643220030409566 1103 10-11 0.13903214328433222 1103 12-13 0.19192728810618576 1103 14-15 0.2414487004221897 1103 16-17 0.2721010605672589 1103 18-19 0.2574688839105832 1103 20-21 0.3443301793218083 1103 22-23 0.2868426382981326 1103 24-25 0.2384827325665526 1103 26-27 0.24296972307715947 1103 28-29 0.2754783261941185 1103 30-31 0.23758461036985068 1103 32-33 0.21800956540459282 1103 34-35 0.29027207527422405 1103 36-37 0.31485850960937256 1103 38-39 0.3853560878841762 1103 40-41 0.3995367969280963 1103 42-43 0.28702100077723003 1103 44-45 0.2802542546936948 1103 46-47 0.2600846281099365 1103 48-49 0.2581292573214853 1103 50-51 0.3946008786586006 1103 52-53 0.36868372008130024 1103 54-55 0.2883100371567622 1103 56-57 0.33071998029750915 1103 58-59 0.25348415523804135 1103 60-61 0.27617593296101717 1103 62-63 0.32516733741965 1103 64-65 0.362142196919109 1103 66-67 0.2678875222968671 1103 68-69 0.17954881826126723 1103 70-71 0.06654640045026383 1103 72-73 0.1209531074812773 1103 74-75 0.24290792857778598 1103 76-77 0.20425537247657033 1103 78-79 0.19768752027097847 1103 80-81 0.11025744259690029 1103 82-83 0.2578033696157789 1103 84-85 0.14253442965722485 1103 86-87 0.3005898967404299 1103 88-89 0.3855247941088038 1103 90-91 0.5384317434062567 1103 92-93 0.4427099508751624 1103 94-95 0.2768562870540876 1103 96-97 0.3206015753038507 1103 98-99 0.20025873824325657 1103 100-101 -0.05032717589726943 1104 1 0.1569143297504354 1104 2 0.152655645439431 1104 3 0.14117605068445727 1104 4 0.07886459067026408 1104 5 0.15238888799795092 1104 6 0.14647126597170512 1104 7 0.08114841708188436 1104 8 0.07977546263641244 1104 9 0.18130284735949687 1104 10-11 0.18445489002385784 1104 12-13 0.17398622847787237 1104 14-15 0.18479937770839427 1104 16-17 0.1711938943172342 1104 18-19 0.16904597245194708 1104 20-21 0.18913450271386267 1104 22-23 0.20925872844792792 1104 24-25 0.23578545721434097 1104 26-27 0.20649945972038353 1104 28-29 0.1761674818633665 1104 30-31 0.35761445359602106 1104 32-33 0.2694244540738566 1104 34-35 0.2722416313077858 1104 36-37 0.32197364826409824 1104 38-39 0.43411972565717605 1104 40-41 0.4882067870449518 1104 42-43 0.4120440130052003 1104 44-45 0.40431995823806943 1104 46-47 0.3845357259143327 1104 48-49 0.4217709060628465 1104 50-51 0.5189497537240086 1104 52-53 0.4250258901101702 1104 54-55 0.5013894608336855 1104 56-57 0.5544862826442696 1104 58-59 0.5140470101108221 1104 60-61 0.5982296410787526 1104 62-63 0.5442112883527344 1104 64-65 0.4009287015796943 1104 66-67 0.5685447030809243 1104 68-69 0.5628790025528829 1104 70-71 0.6054811523057779 1104 72-73 0.4168070307027918 1104 74-75 0.3666156103017144 1104 76-77 0.4810806055056567 1104 78-79 0.6375766740023501 1104 80-81 0.43963509505896425 1104 82-83 0.42108312079303545 1104 84-85 0.3655953466581714 1104 86-87 0.4409230653546601 1104 88-89 0.5823546780302848 1104 90-91 0.5693950594925319 1104 92-93 0.6726435303182186 1104 94-95 0.715225907037425 1104 96-97 0.7150721002733285 1104 98-99 0.6269398420550516 1104 100-101 0.18320793834027782 1105 1 -0.04033967119399762 1105 2 0.03849192516626232 1105 3 4.519113119982876E-5 1105 4 0.01857165764962332 1105 5 0.04740759809266848 1105 6 0.05529377998939111 1105 7 0.07310327476215406 1105 8 0.07338903072885472 1105 9 0.07288429678668962 1105 10-11 0.06512059638450296 1105 12-13 0.0744199534732104 1105 14-15 0.09696431225282254 1105 16-17 0.06492333404612083 1105 18-19 0.07204236535202568 1105 20-21 0.035957357997332906 1105 22-23 0.07864996585383466 1105 24-25 0.06077881046807221 1105 26-27 0.09299208083997001 1105 28-29 0.07288458443754564 1105 30-31 0.17377973734264174 1105 32-33 0.05606191473901134 1105 34-35 0.27619713977395577 1105 36-37 0.17245122194378126 1105 38-39 0.24375810297807732 1105 40-41 0.1793378450085399 1105 42-43 0.12619027316876696 1105 44-45 0.2151507816806415 1105 46-47 0.17429933983956403 1105 48-49 0.15952283758746688 1105 50-51 0.01897872163311831 1105 52-53 0.14121696720052768 1105 54-55 0.16899381475010244 1105 56-57 4.4089429571414485E-4 1105 58-59 0.10293325352855476 1105 60-61 0.22231744955723087 1105 62-63 0.22635197715406008 1105 64-65 0.17415362350522656 1105 66-67 0.10925493272105058 1105 68-69 0.08422874838923633 1105 70-71 0.11157750830154356 1105 72-73 -0.07336094348799094 1105 74-75 -0.12866658871971737 1105 76-77 -0.021026512266754338 1105 78-79 0.09324942392992952 1105 80-81 0.03054075888043073 1105 82-83 0.08807240266666483 1105 84-85 0.21311105083629656 1105 86-87 0.22630815997655063 1105 88-89 0.3227442765668087 1105 90-91 0.40854697435068843 1105 92-93 0.37440819185002283 1105 94-95 0.12381072547212213 1105 96-97 0.19077318319694214 1105 98-99 0.2850343885485458 1105 100-101 0.02377650300022438 1106 1 0.06738688214468169 1106 2 0.05271547191959769 1106 3 0.08443320701435653 1106 4 0.0410865108161218 1106 5 0.0670242575051816 1106 6 0.11163981306374637 1106 7 0.11754887511157364 1106 8 0.05638891522335143 1106 9 0.1621712759505627 1106 10-11 0.1617222867425454 1106 12-13 0.1675043864673924 1106 14-15 0.13789334368179595 1106 16-17 0.10138153749951329 1106 18-19 0.11161531625759835 1106 20-21 0.16814147530197232 1106 22-23 0.19420610956104412 1106 24-25 0.20656955274010613 1106 26-27 0.31933980442535415 1106 28-29 0.25961843298152587 1106 30-31 0.2468270249903597 1106 32-33 0.23637182136313584 1106 34-35 0.16462800131498057 1106 36-37 0.26677561800679683 1106 38-39 0.3312959004334388 1106 40-41 0.3113038787407376 1106 42-43 0.2689627265592378 1106 44-45 0.3373524695908898 1106 46-47 0.38003460887176743 1106 48-49 0.38967910659137317 1106 50-51 0.40328404781377714 1106 52-53 0.49100251707940146 1106 54-55 0.46000220840510053 1106 56-57 0.43325233653111184 1106 58-59 0.3305378452930938 1106 60-61 0.31237349649988744 1106 62-63 0.4776041409699303 1106 64-65 0.4806941418419157 1106 66-67 0.39375845410907573 1106 68-69 0.39471022275964174 1106 70-71 0.37898017196557277 1106 72-73 0.3840786436907493 1106 74-75 0.39306175610169447 1106 76-77 0.4595668035597207 1106 78-79 0.6085114493151345 1106 80-81 0.6230136246484967 1106 82-83 0.7082680429840522 1106 84-85 0.663293985548151 1106 86-87 0.7662747475232194 1106 88-89 0.8825280135447109 1106 90-91 0.858117806380438 1106 92-93 1.034789880173335 1106 94-95 0.8280207098903567 1106 96-97 0.9384077298990334 1106 98-99 0.852877961967593 1106 100-101 0.29301226197465624 1107 1 0.022288914915193914 1107 2 0.042132889298819975 1107 3 -0.01627043875588896 1107 4 0.01646917114118196 1107 5 0.07003023940482933 1107 6 0.06356795163868867 1107 7 0.015871757778405993 1107 8 0.04180722180777252 1107 9 0.06625375696027902 1107 10-11 0.06549386391679946 1107 12-13 0.0866743758495403 1107 14-15 0.12970490849505723 1107 16-17 0.11045151731316594 1107 18-19 0.07053510538067798 1107 20-21 0.12980837660395395 1107 22-23 0.16310835451510286 1107 24-25 0.09283844365318572 1107 26-27 0.0764109515376461 1107 28-29 0.04510931270206697 1107 30-31 0.15353643382788817 1107 32-33 0.25096970316995737 1107 34-35 0.14548927576343118 1107 36-37 0.21565593058151222 1107 38-39 0.15802135890457691 1107 40-41 0.09733728637686312 1107 42-43 0.14736413159597106 1107 44-45 0.1484448564506593 1107 46-47 0.17161731030377325 1107 48-49 0.14470625249994384 1107 50-51 0.21053270352688003 1107 52-53 0.21068057623004677 1107 54-55 0.212036236403506 1107 56-57 0.30290507241259235 1107 58-59 0.24651048286478527 1107 60-61 0.32190325553279564 1107 62-63 0.1250212686925707 1107 64-65 0.2095961150622614 1107 66-67 0.22580006463430635 1107 68-69 0.28549560256097806 1107 70-71 0.3784216724675602 1107 72-73 0.37772831360656056 1107 74-75 0.327034367078177 1107 76-77 0.3565499523116671 1107 78-79 0.6027969472050039 1107 80-81 0.4780293808675147 1107 82-83 0.46126166678145 1107 84-85 0.48454025173906956 1107 86-87 0.47556737553220074 1107 88-89 0.46306598269368493 1107 90-91 0.5479198281370579 1107 92-93 0.6694235990312798 1107 94-95 0.48046852473216006 1107 96-97 0.47831218659205277 1107 98-99 0.5793411251387717 1107 100-101 0.3303792606093374 1108 1 0.03416490701263797 1108 2 0.057881042293224994 1108 3 0.04183582150875509 1108 4 0.02213741768240851 1108 5 0.031894700868100756 1108 6 0.0439369768203548 1108 7 -0.016663427488865068 1108 8 0.05850195289711735 1108 9 0.038682635952589806 1108 10-11 0.04368735861915951 1108 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78-79 -0.06318187945820952 2316 80-81 0.03021019302533645 2316 82-83 -0.10187108840104386 2316 84-85 -0.20365521426056432 2316 86-87 -0.5182010856376813 2316 88-89 -0.6408874000012474 2316 90-91 -0.6536719496822982 2316 92-93 -0.628986611339073 2316 94-95 -0.3155481813632548 2316 96-97 -0.5508835509059331 2316 98-99 -0.566104120006127 2316 100-101 -0.21062251307679603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 2.0 4 22.0 5 45.0 6 90.0 7 231.0 8 561.0 9 1088.0 10 1959.0 11 2988.0 12 4416.0 13 6314.0 14 7826.0 15 10534.0 16 13721.0 17 19619.0 18 19719.0 19 14209.0 20 13077.0 21 13441.0 22 14321.0 23 15193.0 24 17431.0 25 20585.0 26 24172.0 27 29488.0 28 35593.0 29 44149.0 30 53655.0 31 66729.0 32 83722.0 33 109374.0 34 153257.0 35 231108.0 36 391049.0 37 672246.0 38 581147.0 39 58513.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.975641042595115 18.827793132531358 15.01551638256699 23.181049442306534 2 15.765260006860446 20.34480877697731 39.12738353807895 24.762547678083294 3 19.968289658701984 23.69064881730531 29.397485211053887 26.943576312938823 4 13.704746776707141 16.57789699154452 36.023086827102496 33.69426940464585 5 14.436808303994617 36.845754559322536 33.49134811416455 15.226089022518297 6 32.76591273762164 35.231389943113946 16.76679720061605 15.235900118648368 7 29.142689580213215 32.22945078728555 20.206721259803317 18.421138372697914 8 26.478647503789905 34.28059222412058 19.35527919341075 19.88548107867877 9 26.17885994330797 17.490475365911788 20.277558838205138 36.0531058525751 10-11 25.672252279890074 25.922105754098055 26.459702570031276 21.9459393959806 12-13 26.763150987278156 23.2055486873815 27.062059942136496 22.969240383203843 14-15 23.17899014304934 24.84733166762765 25.751791548831722 26.22188664049129 16-17 22.663202459699214 27.896835994608928 26.37394801163514 23.06601353405672 18-19 22.75281886074096 26.803173963977155 28.23643652072046 22.20757065456143 20-21 23.54088316705112 25.540040437823823 28.178548038311597 22.740528356813456 22-23 23.08307472431003 25.42175815574822 28.418993110633657 23.076174009308094 24-25 23.431612569613826 25.62971166034403 28.182952221007014 22.75572354903513 26-27 22.926327635382915 25.701200080393416 28.616175257645153 22.75629702657852 28-29 22.59028679517982 26.701026247976916 28.13571601416166 22.5729709426816 30-31 22.926607377061934 25.80185117558711 27.927032966228353 23.344508481122602 32-33 22.84971340982816 25.896736016716936 28.08418945519496 23.16936111825995 34-35 23.623664817072914 25.896448256438596 27.724703124367867 22.755183802120623 36-37 23.47810101971072 25.49302435693848 28.23780079525462 22.791073828096174 38-39 23.002335218779148 25.931309232947914 28.17381497487649 22.892540573396445 40-41 23.152245827049516 26.061201199024175 27.86200750506825 22.924545468858067 42-43 23.016519545566048 26.06963325290795 27.296205849940137 23.61764135158587 44-45 23.20361498293716 25.85613031276902 27.613887576534758 23.326367127759063 46-47 23.749098045848374 26.314143995630353 27.228600119564984 22.708157838956293 48-49 23.566958377645214 26.207311872044986 27.11021256889315 23.11551718141665 50-51 22.984101985686774 26.628066564675123 27.46279743110171 22.925034018536394 52-53 23.56902547957241 26.664610484697615 26.634664665397565 23.131699370332406 54-55 23.024501016070793 26.247446094799347 26.972021170002503 23.756031719127353 56-57 23.342977250337807 26.366094462551448 26.896607520644487 23.394320766466258 58-59 23.765066730466174 26.025421809876608 26.873022930663616 23.3364885289936 60-61 23.201578582014797 26.257747192558007 27.56744743917941 22.97322678624778 62-63 22.827833300109628 26.519197351915174 27.379425868763256 23.273543479211938 64-65 22.704385247176965 26.72747474414663 27.062871012558826 23.505268996117582 66-67 22.888722150517403 26.343226768232668 26.896624060517855 23.87142702073208 68-69 22.85812609142928 26.19294822277788 27.242770482260724 23.706155203532113 70-71 23.237805088994527 26.279007490297356 26.87305482628225 23.61013259442587 72-73 23.090725914948777 26.079266335017447 27.232564345964143 23.59744340406963 74-75 22.74248691518271 27.070699564101787 26.619425750686137 23.56738777002937 76-77 23.029736363854823 26.337877721418536 26.72824052952862 23.90414538519802 78-79 23.06423252968691 26.52417583745151 26.873331329617063 23.538260303244517 80-81 23.280801872666583 26.50883798168483 26.71043007044466 23.499930075203935 82-83 23.103371523628784 26.397046548815634 26.965745882242686 23.533836045312903 84-85 22.8693356020883 26.67281934660296 26.814201634865416 23.643643416443325 86-87 23.01643380465245 26.6934888914869 26.563842844436103 23.726234459424546 88-89 22.72402126702288 26.707746789508818 26.276624212999582 24.291607730468716 90-91 22.87255146443069 26.386159692491518 26.536187138105188 24.20510170497261 92-93 23.159110719318086 26.43669408526355 26.67324034513093 23.730954850287432 94-95 22.82703440216928 26.272962266451067 26.780375318357258 24.1196280130224 96-97 22.538741931929295 26.14521666963806 27.214186845004086 24.101854553428556 98-99 22.59142889829539 26.588847957899553 26.330131563894295 24.489591579910766 100-101 22.602911612127706 25.628779978763205 27.05482304285854 24.713485366250552 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 418.0 1 288.0 2 207.0 3 280.0 4 262.5 5 282.5 6 517.0 7 1152.5 8 1940.5 9 2330.5 10 2219.5 11 2070.0 12 2198.0 13 2425.0 14 2649.0 15 2792.5 16 2940.5 17 3094.5 18 3282.0 19 3562.0 20 3894.0 21 4530.0 22 5443.5 23 6796.5 24 8518.5 25 10572.0 26 13366.0 27 16708.0 28 19903.5 29 23364.0 30 27244.5 31 31940.0 32 37750.5 33 40255.0 34 41494.5 35 43982.0 36 47477.5 37 52441.5 38 57067.0 39 60100.5 40 63631.5 41 70190.5 42 75427.5 43 79185.5 44 84461.5 45 91615.5 46 97042.0 47 108623.5 48 129067.0 49 127469.5 50 110560.5 51 103480.5 52 102621.0 53 105250.5 54 106088.5 55 100250.0 56 91410.0 57 83974.5 58 77600.5 59 69413.0 60 57518.0 61 46823.5 62 38618.0 63 29650.0 64 21755.0 65 16450.5 66 12777.5 67 10466.5 68 8284.5 69 6397.0 70 4638.5 71 3252.5 72 2578.0 73 2143.5 74 1674.5 75 1359.0 76 982.0 77 543.0 78 311.0 79 197.0 80 104.0 81 57.0 82 39.0 83 20.0 84 10.5 85 8.5 86 6.0 87 6.0 88 4.5 89 2.5 90 3.5 91 3.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3410820247905898 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.518227588875536E-4 16-17 0.001738906963352261 18-19 3.6608567649521286E-5 20-21 0.0 22-23 0.0 24-25 4.942156632685374E-4 26-27 0.0012446913000837237 28-29 0.0 30-31 0.0034229010752302403 32-33 0.0 34-35 0.010268703225690722 36-37 0.0284631613475028 38-39 0.021727184899990884 40-41 0.013911255706818088 42-43 0.010653093186010695 44-45 0.0029469896957864637 46-47 0.0011714741647846812 48-49 5.857370823923406E-4 50-51 7.321713529904257E-5 52-53 3.6608567649521286E-5 54-55 0.0 56-57 0.0 58-59 0.004667592375313965 60-61 0.010579876050711652 62-63 0.004301506698818751 64-65 0.002050079788373192 66-67 0.004008638157622581 68-69 0.003331379656106437 70-71 1.8304283824760643E-5 72-73 0.0 74-75 0.0 76-77 0.2171437190131355 78-79 3.6608567649521286E-4 80-81 0.003477813926704522 82-83 6.223456500418619E-4 84-85 0.0 86-87 0.11048465716625525 88-89 0.40800248645391474 90-91 0.39410953503092144 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014094298545065697 100-101 7.870842044647077E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731601.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.25871997160434 #Duplication Level Percentage of deduplicated Percentage of total 1 69.95620977638707 33.060409280970866 2 15.393998840427864 14.550013608859647 3 5.803317012617043 8.227720008171492 4 2.832597150337929 5.354596620807386 5 1.6050737635336827 3.79268657623037 6 1.0012543202279756 2.8390798524007774 7 0.6749285883688225 2.2327382810986682 8 0.465356130639183 1.7593708051957182 9 0.35136691724817104 1.4944635674565474 >10 1.7665491295185682 14.770160483377362 >50 0.0980767221628966 3.1874616431282115 >100 0.04452814598273497 3.7971830498297257 >500 0.0037980646535156573 1.2652331461852402 >1k 0.0026353918003812118 2.4302440026083736 >5k 2.3253457062187162E-4 0.7640850664778739 >10k+ 7.751152354062388E-5 0.4745540072017286 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12955 0.47426399389954826 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8057 0.294955229552193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6570 0.24051828945735484 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6232 0.22814459359181669 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4866 0.1781372901825706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4285 0.15686771237819871 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4079 0.14932634744239734 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3705 0.13563474314147636 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3148 0.11524377096069302 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2992 0.10953283440736769 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2959 0.1083247516749335 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2787 0.10202807803921583 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.6608567649521286E-5 2 0.0 0.0 0.0 0.0 3.6608567649521286E-5 3 0.0 0.0 0.0 0.0 3.6608567649521286E-5 4 0.0 0.0 0.0 0.0 3.6608567649521286E-5 5 0.0 0.0 0.0 0.0 7.321713529904257E-5 6 0.0 0.0 0.0 0.0 1.0982570294856386E-4 7 0.0 0.0 0.0 0.0 1.0982570294856386E-4 8 0.0 0.0 0.0 0.0 1.0982570294856386E-4 9 0.0 0.0 0.0 0.0 1.0982570294856386E-4 10-11 0.0 0.0 0.0 0.0 1.0982570294856386E-4 12-13 0.0 0.0 0.0 0.0 3.843899603199735E-4 14-15 0.0 0.0 0.0 0.0 4.942156632685374E-4 16-17 0.0 0.0 0.0 0.0 7.504756368151863E-4 18-19 0.0 0.0 0.0 3.6608567649521286E-5 8.786056235885109E-4 20-21 0.0 0.0 0.0 1.281299867733245E-4 9.518227588875534E-4 22-23 0.0 0.0 0.0 2.3795568972188836E-4 0.0010616484618361174 24-25 0.0 0.0 0.0 3.294771088456916E-4 0.0010616484618361174 26-27 0.0 0.0 0.0 6.589542176913832E-4 0.0011165613133103992 28-29 0.0 0.0 0.0 0.002489382600167448 0.00113486559713516 30-31 0.0 0.0 0.0 0.008072189166719444 0.0011531698809599206 32-33 0.0 0.0 0.0 0.017425678201172135 0.0011714741647846812 34-35 0.0 0.0 0.0 0.03375309937285863 0.0011714741647846812 36-37 0.0 0.0 0.0 0.05842727396863598 0.0011714741647846812 38-39 0.0 0.0 0.0 0.09693948713593237 0.0012996041515580057 40-41 0.0 0.0 0.0 0.14121754970802836 0.0013179084353827663 42-43 0.0 0.0 0.0 0.18820464628618894 0.0013545170030322875 44-45 0.0 0.0 0.0 0.2471078316342687 0.0014094298545065695 46-47 0.0 0.0 0.0 0.31926331847147515 0.0014460384221560909 48-49 0.0 0.0 0.0 0.39367023221912717 0.0014643427059808515 50-51 0.0 0.0 0.0 0.4753256423613844 0.0015009512736303729 52-53 0.0 0.0 0.0 0.5576217024375083 0.001592472692754176 54-55 0.0 0.0 0.0 0.6587711748531355 0.001647385544228458 56-57 0.0 0.0 0.0 0.7795428395289064 0.001647385544228458 58-59 0.0 0.0 0.0 0.9067576121109928 0.001647385544228458 60-61 0.0 0.0 0.0 1.0503730230000647 0.001647385544228458 62-63 0.0 0.0 0.0 1.1985278962776773 0.0017206026795275006 64-65 0.0 0.0 0.0 1.363284754984348 0.0018121240986513037 66-67 0.0 0.0 0.0 1.5584816376915955 0.0018853412339503463 68-69 0.0 0.0 0.0 1.7775839150739805 0.0019402540854246283 70-71 0.0 0.0 0.0 2.0118238351794426 0.001958558369249389 72-73 0.0 0.0 0.0 2.252964470286839 0.002104992639847474 74-75 0.0 0.0 0.0 2.5185047157326417 0.002251426910445559 76-77 0.0 0.0 0.0 2.8033742848973917 0.002306339761919841 78-79 0.0 0.0 0.0 3.139971760150915 0.0023246440457446015 80-81 0.0 0.0 0.0 3.4973629018293666 0.0023978611810436443 82-83 0.0 0.0 0.0 3.8641441411099207 0.0025442954516417295 84-85 0.0 0.0 0.0 4.255013817903859 0.002580904019291251 86-87 0.0 0.0 0.0 4.667336115340417 0.0026358168707655326 88-89 0.0 0.0 0.0 5.121117615640058 0.002690729722239815 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9780 0.0 21.958544 1 TACATGG 9790 0.0 21.3247 2 TAAGGTG 1470 0.0 21.30294 5 GAGTACT 8620 0.0 20.75328 12-13 GTAAGGT 1365 0.0 20.508425 4 ACATGGG 9960 0.0 20.34143 3 CGTTAGG 140 4.890451E-4 20.334625 2 GTACTTT 8890 0.0 19.936161 14-15 AGAGTAC 14385 0.0 19.214577 10-11 AGTACTT 8870 0.0 19.178658 12-13 GTAGGAC 480 0.0 18.781286 3 GGTAAGG 1570 0.0 17.226147 3 TACTTTT 9970 0.0 17.015064 14-15 CATGGGG 6285 0.0 16.759483 4 ACTTTTT 10835 0.0 16.488033 16-17 AGGTAAG 1535 0.0 16.38251 2 ATACAGT 1755 0.0 16.221352 6 TATAGCG 205 3.3623708E-4 16.20157 5 TTACGGT 235 5.1874267E-5 16.152327 4 ACCGTCC 330 2.306515E-7 15.815819 8 >>END_MODULE