FastQCFastQC Report
Fri 27 May 2016
SRR939086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686751
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113510.42248053504027727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74600.2776587782046047No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67250.25030231681313225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57800.21512972359552487No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53780.20016741409978073No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43750.16283607971114555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37710.14035539579216683No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33260.12379264025583316No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29050.10812315692820065No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27070.10075366120641623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96050.023.2832621
TACATGG96150.022.9563852
GAGTACT76550.021.5529112-13
ACATGGG98500.021.3515663
AGTACTT79600.019.92416412-13
AGAGTAC133000.019.73704310-11
GTACTTT82800.019.66855414-15
TAAGGTG13000.019.311125
CATGGGG61800.019.1617224
GTAAGGT12900.017.9924134
GTAGGAC5050.017.8215393
GTATCAA219850.017.256021
TACTTTT91850.017.11209714-15
GTGTAAG10450.016.8114721
ACTTTTT99450.016.42399616-17
GTATTGG9650.016.2370411
TAGGACT8550.016.0662634
GAGTACA77650.015.8983531
GGACCGA3905.7752914E-915.78043656
GTATTAG7250.015.7178651
Produced by FastQC (version 0.11.5)