##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939086_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2686751 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.106311303131555 33.0 31.0 34.0 30.0 34.0 2 32.216046444199705 34.0 31.0 34.0 30.0 34.0 3 32.282003058712924 34.0 31.0 34.0 30.0 34.0 4 35.71175334074501 37.0 35.0 37.0 35.0 37.0 5 35.74649716330244 37.0 35.0 37.0 35.0 37.0 6 35.83351564770982 37.0 37.0 37.0 35.0 37.0 7 35.76295347056724 37.0 36.0 37.0 35.0 37.0 8 35.75639350278459 37.0 36.0 37.0 35.0 37.0 9 37.433407859529964 39.0 38.0 39.0 35.0 39.0 10-11 37.338128095979116 39.0 37.5 39.0 34.5 39.0 12-13 37.24254331718868 39.0 37.0 39.0 34.0 39.0 14-15 38.5315529797886 40.0 38.0 41.0 34.0 41.0 16-17 38.44285607412075 40.0 38.0 41.0 34.0 41.0 18-19 38.333385006649294 40.0 38.0 41.0 34.0 41.0 20-21 38.304411350363324 40.0 38.0 41.0 34.0 41.0 22-23 38.35819433955733 40.0 38.0 41.0 34.0 41.0 24-25 38.294729210112884 40.0 38.0 41.0 34.0 41.0 26-27 38.1741320650853 40.0 38.0 41.0 33.5 41.0 28-29 38.034590849691696 40.0 38.0 41.0 33.0 41.0 30-31 37.83580782141702 40.0 38.0 41.0 33.0 41.0 32-33 37.63322633917323 40.0 37.5 41.0 32.5 41.0 34-35 37.3186951451772 40.0 37.0 41.0 31.5 41.0 36-37 37.04715881747136 40.0 37.0 41.0 31.0 41.0 38-39 36.850168474860524 40.0 36.5 41.0 30.0 41.0 40-41 36.666401910709254 40.0 36.0 41.0 30.0 41.0 42-43 36.461172620760166 40.0 36.0 41.0 30.0 41.0 44-45 36.18541483747471 39.0 35.5 41.0 29.5 41.0 46-47 36.05919361340146 39.0 36.0 41.0 28.5 41.0 48-49 35.924342263201915 39.0 35.0 41.0 28.0 41.0 50-51 34.78081556497048 38.0 34.0 40.0 25.5 40.5 52-53 34.92262103931477 38.5 34.5 39.5 26.5 40.5 54-55 35.43919644954073 39.0 35.0 40.5 27.0 41.0 56-57 35.31930554785315 39.0 35.0 41.0 26.5 41.0 58-59 35.09118187729343 39.0 35.0 41.0 26.0 41.0 60-61 34.758915880183906 38.0 35.0 40.0 26.0 41.0 62-63 34.37221834103718 37.5 34.0 40.0 25.5 41.0 64-65 33.980979257102724 37.0 34.0 40.0 24.5 41.0 66-67 33.559771634959844 36.5 34.0 39.0 23.0 41.0 68-69 33.134018187766564 36.0 33.0 39.0 22.0 41.0 70-71 32.68135603187642 35.0 33.0 38.5 20.5 40.0 72-73 32.24016721311354 35.0 32.5 37.5 20.0 40.0 74-75 31.81199243993954 35.0 32.0 37.0 18.5 39.0 76-77 31.3851142141568 35.0 32.0 36.5 17.0 39.0 78-79 30.964761714055378 35.0 31.0 36.0 12.0 38.5 80-81 30.525320917345894 35.0 31.0 35.5 7.5 37.0 82-83 30.13262635800638 34.0 31.0 35.0 4.5 37.0 84-85 29.766880518514743 34.0 30.0 35.0 2.0 36.5 86-87 29.380617519077873 34.0 30.0 35.0 2.0 36.0 88-89 28.95507138547636 34.0 29.0 35.0 2.0 36.0 90-91 28.540680546876132 34.0 29.0 35.0 2.0 35.0 92-93 28.134213032767086 34.0 29.0 35.0 2.0 35.0 94-95 27.635565595769762 34.0 27.0 35.0 2.0 35.0 96-97 27.020202095393284 34.0 26.5 35.0 2.0 35.0 98-99 26.286974676849475 33.0 25.0 35.0 2.0 35.0 100-101 23.240528057866175 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20600876390602707 1101 2 0.3536389583636961 1101 3 0.19089882067783037 1101 4 0.33781522406967923 1101 5 0.2289488530488697 1101 6 0.2374233965478112 1101 7 0.24639521366417938 1101 8 0.3097180245440825 1101 9 0.3561260996094404 1101 10-11 0.2912884578901114 1101 12-13 0.3362361512122476 1101 14-15 0.5089404575197847 1101 16-17 0.4402282086934832 1101 18-19 0.5201024206168512 1101 20-21 0.5691629208591067 1101 22-23 0.4609060617969192 1101 24-25 0.49595099968243517 1101 26-27 0.4277887366331754 1101 28-29 0.45137949786676757 1101 30-31 0.5019397058440376 1101 32-33 0.43961632632221637 1101 34-35 0.5482400781307106 1101 36-37 0.35980023390249016 1101 38-39 0.39009822934716 1101 40-41 0.4583976408257868 1101 42-43 0.4127777735857734 1101 44-45 0.28620763564094176 1101 46-47 0.44305109085020433 1101 48-49 0.4715723119929365 1101 50-51 0.5617055897226209 1101 52-53 0.4590500460470679 1101 54-55 0.5675683316439688 1101 56-57 0.5394231767009074 1101 58-59 0.44227736667469486 1101 60-61 0.40562603152195464 1101 62-63 0.26927291130676423 1101 64-65 0.24789386510885691 1101 66-67 0.2493229957338272 1101 68-69 0.24855150505364776 1101 70-71 0.20410533946927245 1101 72-73 0.09250195560968422 1101 74-75 0.20063051404112642 1101 76-77 0.18990094393414836 1101 78-79 -0.035109099019255297 1101 80-81 -0.0783771045206727 1101 82-83 -0.15797840230152715 1101 84-85 -0.22887090898795748 1101 86-87 -0.11536410869572222 1101 88-89 -0.20933004633053187 1101 90-91 -0.2319376520509735 1101 92-93 -0.4951239159813525 1101 94-95 -0.42693773252271683 1101 96-97 -0.5432995887594636 1101 98-99 -0.776011197678713 1101 100-101 -0.7936337018780186 1102 1 0.30727475385206304 1102 2 0.26716265972623887 1102 3 0.31854693768652 1102 4 0.19388590117181792 1102 5 0.3446285376371918 1102 6 0.36192157855807494 1102 7 0.2756107048735643 1102 8 0.39453993784989194 1102 9 0.4498501466688154 1102 10-11 0.4536256605042652 1102 12-13 0.4241294987490889 1102 14-15 0.535094235387561 1102 16-17 0.4829054810339244 1102 18-19 0.4364044622237273 1102 20-21 0.5115627993663239 1102 22-23 0.49093388921848913 1102 24-25 0.485588531559614 1102 26-27 0.4644493286610256 1102 28-29 0.5237680267770628 1102 30-31 0.5852534139855194 1102 32-33 0.5506028388632629 1102 34-35 0.3971570976840013 1102 36-37 0.40465773666835503 1102 38-39 0.30853794956161806 1102 40-41 0.29982829163382263 1102 42-43 0.29870643766412286 1102 44-45 0.27851070876257467 1102 46-47 0.265377075924782 1102 48-49 0.30496672185791596 1102 50-51 0.19987831195315664 1102 52-53 0.19324993247774103 1102 54-55 0.21954251589627205 1102 56-57 0.21511156240232765 1102 58-59 0.27238061352512233 1102 60-61 0.1606968509525828 1102 62-63 0.11275375271760879 1102 64-65 0.26719588323886256 1102 66-67 0.26726904149609965 1102 68-69 0.2737441987597933 1102 70-71 0.2856203162418538 1102 72-73 0.2670284199057775 1102 74-75 0.14221897340120293 1102 76-77 0.0561605804535823 1102 78-79 -0.04069365828513227 1102 80-81 -0.16053842873324342 1102 82-83 -0.3211882840074729 1102 84-85 -0.18424120517795473 1102 86-87 -0.2699941133030599 1102 88-89 -0.3051070351878238 1102 90-91 -0.3203973789563399 1102 92-93 -0.3595041194083848 1102 94-95 -0.45998930679324346 1102 96-97 -0.6734784384517702 1102 98-99 -0.7372912443976745 1102 100-101 -0.964498875709527 1103 1 0.15318775475471114 1103 2 0.2399222028338741 1103 3 0.33918075164397266 1103 4 0.2915098862312604 1103 5 0.2975473932158792 1103 6 0.3049403122329508 1103 7 0.3002964070517251 1103 8 0.2918719407160282 1103 9 0.399028136980931 1103 10-11 0.4354399443676513 1103 12-13 0.42598829933863414 1103 14-15 0.46234797070523825 1103 16-17 0.5196231903015445 1103 18-19 0.46609104843721383 1103 20-21 0.49940570306804233 1103 22-23 0.5835219151568722 1103 24-25 0.5702356296689217 1103 26-27 0.5037026445093105 1103 28-29 0.5232861022572166 1103 30-31 0.5653153751982032 1103 32-33 0.5772933685185393 1103 34-35 0.5186176457286393 1103 36-37 0.5156277918507755 1103 38-39 0.6247461108898378 1103 40-41 0.4825736042781088 1103 42-43 0.4986958077656851 1103 44-45 0.3047826637977451 1103 46-47 0.594304608650198 1103 48-49 0.5923950595207472 1103 50-51 0.5573297633816736 1103 52-53 0.6306994225647315 1103 54-55 0.6847269142118151 1103 56-57 0.7162824618205832 1103 58-59 0.7072427791754521 1103 60-61 0.636370847357739 1103 62-63 0.5160716052920122 1103 64-65 0.3844316729973727 1103 66-67 0.31885715548011007 1103 68-69 0.24211738765058755 1103 70-71 0.22014139457883175 1103 72-73 0.26694144800950426 1103 74-75 0.288302947451367 1103 76-77 0.29714091478357574 1103 78-79 0.24076851466653792 1103 80-81 0.22896735615053743 1103 82-83 0.18569967543141175 1103 84-85 0.07936524377026188 1103 86-87 0.22802826030673273 1103 88-89 0.2651950686730835 1103 90-91 0.12604296512309077 1103 92-93 -0.005540312601958419 1103 94-95 -0.29391754459946284 1103 96-97 -0.3534712516382861 1103 98-99 -0.3568529060404906 1103 100-101 -0.4751581226832293 1104 1 0.1306520425695865 1104 2 0.1979587730194865 1104 3 0.2611185291526965 1104 4 0.2609059770234978 1104 5 0.32800150919333504 1104 6 0.30796231756359305 1104 7 0.3184121010827994 1104 8 0.23073239774532084 1104 9 0.358784147713763 1104 10-11 0.3984054169420759 1104 12-13 0.3746539357397296 1104 14-15 0.42165468773636405 1104 16-17 0.3706335293020331 1104 18-19 0.38818532633581526 1104 20-21 0.4602455219953683 1104 22-23 0.4657112593032906 1104 24-25 0.37320611025582906 1104 26-27 0.4268989603181481 1104 28-29 0.46609896803388295 1104 30-31 0.4599283566131831 1104 32-33 0.35520091359150996 1104 34-35 0.4897911338058947 1104 36-37 0.4095872551541788 1104 38-39 0.14973991106669615 1104 40-41 0.24606789338970714 1104 42-43 0.3235608648745725 1104 44-45 0.4006529108723029 1104 46-47 0.5439284229877686 1104 48-49 0.5987414942870899 1104 50-51 0.471869027953673 1104 52-53 0.4589937299748357 1104 54-55 0.4562096883342832 1104 56-57 0.5215698360914303 1104 58-59 0.47309970823881287 1104 60-61 0.3872330613188808 1104 62-63 0.44510684746003193 1104 64-65 0.43765822949693955 1104 66-67 0.3618803165059461 1104 68-69 0.2501090542627935 1104 70-71 0.33298101332324137 1104 72-73 0.3641698948961931 1104 74-75 0.22536287609407069 1104 76-77 0.24205896877174027 1104 78-79 0.2458828958141197 1104 80-81 0.18284927093109005 1104 82-83 0.216313989335422 1104 84-85 0.20973866745877956 1104 86-87 0.2642994613801548 1104 88-89 0.18124800157637821 1104 90-91 0.27036839856897643 1104 92-93 0.22337512937750148 1104 94-95 0.2179025342973695 1104 96-97 0.16168615507802286 1104 98-99 0.11407181520823784 1104 100-101 0.1618847972907247 1105 1 0.21532973892055196 1105 2 0.2694614927941288 1105 3 0.314134465902697 1105 4 0.33589085330340396 1105 5 0.3546982641760934 1105 6 0.3395106388680915 1105 7 0.31703487367111904 1105 8 0.31199829594791595 1105 9 0.404702706002837 1105 10-11 0.3630768725234148 1105 12-13 0.35207738609691575 1105 14-15 0.43359007647627124 1105 16-17 0.3980449289193686 1105 18-19 0.4905880891737624 1105 20-21 0.4671874633770443 1105 22-23 0.4242710187475751 1105 24-25 0.5576257594320921 1105 26-27 0.5051069313045602 1105 28-29 0.4472743650970301 1105 30-31 0.6578340228560862 1105 32-33 0.5494323932510454 1105 34-35 0.5604222748803807 1105 36-37 0.5330969429011319 1105 38-39 0.3962372541654844 1105 40-41 0.4312290497883495 1105 42-43 0.4786022407447206 1105 44-45 0.5319618141651645 1105 46-47 0.4878237044702658 1105 48-49 0.5520647955943403 1105 50-51 0.4329769907731844 1105 52-53 0.5417115385731606 1105 54-55 0.3769774460433908 1105 56-57 0.44094456486661926 1105 58-59 0.5116401199666072 1105 60-61 0.5006733537701322 1105 62-63 0.3841917297445079 1105 64-65 0.3011262577780016 1105 66-67 0.3024853052294745 1105 68-69 0.4108706643480886 1105 70-71 0.21688032668888724 1105 72-73 0.21462819725716287 1105 74-75 0.34131103645590244 1105 76-77 0.433688353548618 1105 78-79 0.4094306469331599 1105 80-81 0.3161410157667781 1105 82-83 0.33863348979441454 1105 84-85 0.3000295635032231 1105 86-87 0.23196513909330818 1105 88-89 0.3264117627312153 1105 90-91 0.2855357807673826 1105 92-93 0.301343665719088 1105 94-95 0.42822054575397317 1105 96-97 0.4138298810669845 1105 98-99 0.28008111082793974 1105 100-101 -0.05860581835154832 1106 1 0.16791050899616522 1106 2 0.17829140760579065 1106 3 0.28842009607734553 1106 4 0.2509186212772079 1106 5 0.25329283299527816 1106 6 0.20146125208627552 1106 7 0.21513415772420785 1106 8 0.27415252264211887 1106 9 0.2622743678207158 1106 10-11 0.29115972903866805 1106 12-13 0.3605671040045806 1106 14-15 0.2855304216745438 1106 16-17 0.3007951504388018 1106 18-19 0.4242561629754036 1106 20-21 0.3818094763424398 1106 22-23 0.3708046076922713 1106 24-25 0.34246609883964396 1106 26-27 0.3151710465807085 1106 28-29 0.29278983813603787 1106 30-31 0.2984704718681215 1106 32-33 0.21597060183418648 1106 34-35 0.16208489181498464 1106 36-37 0.3971396570875285 1106 38-39 0.41642010838386767 1106 40-41 0.4525614758005361 1106 42-43 0.4332257774143926 1106 44-45 0.5048068806145167 1106 46-47 0.38752627480733537 1106 48-49 0.41608057806501364 1106 50-51 0.3575923686696427 1106 52-53 0.2682312071367505 1106 54-55 0.3803431017489842 1106 56-57 0.36353877006720836 1106 58-59 0.41483240982885405 1106 60-61 0.37880192141371083 1106 62-63 0.35500769764772855 1106 64-65 0.3381785564201607 1106 66-67 0.18759331192342898 1106 68-69 0.22290532392958795 1106 70-71 0.15085136207344618 1106 72-73 0.26993025141109683 1106 74-75 0.15746641029560848 1106 76-77 0.08680130403211095 1106 78-79 0.1551751123442635 1106 80-81 0.1456827463389807 1106 82-83 0.27337826609295135 1106 84-85 0.2743514261362705 1106 86-87 0.4432227741942789 1106 88-89 0.3988352709972318 1106 90-91 0.3417216481028831 1106 92-93 0.3842694917301728 1106 94-95 0.4645966399661283 1106 96-97 0.3416497287547884 1106 98-99 0.41018248730550155 1106 100-101 0.3628880685533602 1107 1 0.01014445349373716 1107 2 0.16629850196584783 1107 3 0.1049682040110298 1107 4 0.09240888198690556 1107 5 0.14203236006679276 1107 6 0.1344573828287281 1107 7 0.13621929313585923 1107 8 0.11551203595269754 1107 9 0.07781018797164307 1107 10-11 0.12616748424492386 1107 12-13 0.17163340313609154 1107 14-15 0.1525734533327281 1107 16-17 0.2290503743074055 1107 18-19 0.27739916972047496 1107 20-21 0.22271307378139937 1107 22-23 0.17163953647357744 1107 24-25 0.1696452376130182 1107 26-27 0.2580203693215495 1107 28-29 0.25484357377159483 1107 30-31 0.2668135283157653 1107 32-33 0.24384684370265575 1107 34-35 0.353473274645836 1107 36-37 0.35015358554922926 1107 38-39 0.33671766844575757 1107 40-41 0.26707774442431287 1107 42-43 0.16251807852533773 1107 44-45 0.18665425790823775 1107 46-47 0.34770457345911865 1107 48-49 0.32812497901628745 1107 50-51 0.19608455751710352 1107 52-53 0.23790414699973894 1107 54-55 0.24009735227583207 1107 56-57 0.21427098641180464 1107 58-59 0.2510042187113939 1107 60-61 0.10404553503690295 1107 62-63 0.15982733114700665 1107 64-65 0.05043212230046379 1107 66-67 0.11486267567505593 1107 68-69 0.09505275877688746 1107 70-71 0.1161793464060068 1107 72-73 0.1742549920954275 1107 74-75 0.1442745231871818 1107 76-77 0.13521292404493224 1107 78-79 0.16459557063398833 1107 80-81 0.20509244810509486 1107 82-83 0.08725408882791541 1107 84-85 -0.04828601781699149 1107 86-87 -0.08347967748946061 1107 88-89 -0.08389200847394562 1107 90-91 -0.18421331943899588 1107 92-93 -0.35575584173751196 1107 94-95 -0.1760891069600703 1107 96-97 0.00922802110102694 1107 98-99 -0.01607257954258401 1107 100-101 0.12389356859879541 1108 1 0.019292529075201514 1108 2 0.08222977224225758 1108 3 0.17032723861181864 1108 4 0.1911848932756257 1108 5 0.17253678228787095 1108 6 0.2434359844267675 1108 7 0.03226630203339198 1108 8 0.1572793793719498 1108 9 0.13050758922143046 1108 10-11 0.1654458090235309 1108 12-13 0.24872124834388387 1108 14-15 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-1.8455389868800083 2316 68-69 -1.8295330240936565 2316 70-71 -1.8888267943814867 2316 72-73 -2.1320748106721297 2316 74-75 -1.85196658082695 2316 76-77 -1.83131338392241 2316 78-79 -1.5535925787449436 2316 80-81 -1.3573790483779753 2316 82-83 -1.463334173919403 2316 84-85 -1.5250552975222114 2316 86-87 -1.6023178259961561 2316 88-89 -1.751267775052881 2316 90-91 -1.9222375377714656 2316 92-93 -1.9434562216031601 2316 94-95 -1.9199140529435716 2316 96-97 -2.062610640100818 2316 98-99 -2.382982649912993 2316 100-101 -2.0807031183891453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8474.0 3 2362.0 4 2371.0 5 2683.0 6 3019.0 7 3671.0 8 4432.0 9 5554.0 10 6866.0 11 8408.0 12 10833.0 13 16507.0 14 14093.0 15 13796.0 16 16340.0 17 22113.0 18 19319.0 19 15062.0 20 14943.0 21 15679.0 22 16997.0 23 18847.0 24 21221.0 25 23537.0 26 26991.0 27 31634.0 28 36787.0 29 44663.0 30 53199.0 31 65652.0 32 82651.0 33 108230.0 34 151490.0 35 228037.0 36 378098.0 37 592021.0 38 517506.0 39 82637.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65809709655022 18.330768615627914 14.630213545446324 23.380920742375537 2 15.368075709403008 19.904796826859013 39.7523927492357 24.974734714502276 3 19.890536721847973 23.62631486176227 29.189400091002014 27.293748325387735 4 13.455721545821413 16.200027132227866 36.262781590038 34.08146973191272 5 14.463052329178424 36.69341192922196 33.53008394792277 15.313451793676848 6 32.74675600163443 34.897291232963354 17.03957236620334 15.316380399198875 7 29.06522319767223 32.170988642404296 20.40656981537062 18.35721834455285 8 26.414198926371395 34.03062199459796 19.582342009304384 19.97283706972626 9 26.28596184011679 17.51599059357128 20.45823746054735 35.73981010576458 10-11 25.590815959168793 25.75908971626642 26.633349642322813 22.016744682241967 12-13 26.77756530235592 23.165859779507755 27.110123380816532 22.946451537319795 14-15 23.275163660405823 24.66684049279708 25.809027163436916 26.248968683360186 16-17 22.722418503818957 27.809311135405746 26.411057741555183 23.05721261922012 18-19 22.731537317450016 26.788880434590272 28.1938482715202 22.285733976439516 20-21 23.588548464214742 25.526629050682857 28.08831070233908 22.796511782763314 22-23 23.15677823629444 25.42758132637343 28.281141230230855 23.13449920710127 24-25 23.42033212707989 25.595444803472102 28.107543356564147 22.87667971288386 26-27 22.91258037207389 25.667960121739284 28.544047026419573 22.875412479767252 28-29 22.64952782745112 26.606645085077872 28.088021137711266 22.655805949759745 30-31 22.929265477199245 25.78475474149918 27.82281191201023 23.463167869291336 32-33 22.84236443842408 25.909471754023418 27.971316094080112 23.27684771347239 34-35 23.656233449885974 25.862772150168407 27.63907324590267 22.84192115404295 36-37 23.491284222911037 25.429165155752347 28.170010511847256 22.909540109489367 38-39 23.086059440991804 25.889312942112277 28.02956054837122 22.9950670685247 40-41 23.117913374193293 26.08516032409683 27.805325708933758 22.99160059277612 42-43 23.018085676160197 26.043601899990293 27.20635540055086 23.73195702329866 44-45 23.148869836321122 25.865176619997314 27.509561311571346 23.476392232110214 46-47 23.7379630611836 26.315553153359456 27.15342194144217 22.79306184401478 48-49 23.56711470360578 26.19254543219546 27.06280568702999 23.17753417716877 50-51 22.947609560858496 26.595197717703513 27.402114057946502 23.05507866349149 52-53 23.51770070452576 26.617793653430805 26.67577446195157 23.18873118009187 54-55 22.988559721486624 26.20214968952733 27.02529373346776 23.783996855518286 56-57 23.26491962271152 26.31275174011144 26.96026246805556 23.462066169121478 58-59 23.730186024757547 25.957553523750963 26.906499898359904 23.40576055313159 60-61 23.18486423805596 26.173255002628903 27.62899342577583 23.012887333539307 62-63 22.81991001823429 26.422865664092516 27.4528560942706 23.30436822340259 64-65 22.709983188434542 26.66662300836275 27.141594170493782 23.481799632708928 66-67 22.869775581480646 26.313206126411504 26.94341482620327 23.873603465904583 68-69 22.836711761451557 26.12810577198773 27.33041917862161 23.704763287939098 70-71 23.239799446458452 26.195626726074412 26.9319915686815 23.632582258785632 72-73 23.146494724773774 25.987368198870875 27.326276245848014 23.539860830507337 74-75 22.81591995672082 27.01743237019942 26.75588225772557 23.41076541535419 76-77 23.036851771441675 26.31615674317468 26.752934692464137 23.89405679291951 78-79 23.123532512070014 26.450917029162913 26.924553025199366 23.500997433567708 80-81 23.373585739945423 26.469288282260766 26.702382018974824 23.454743958818984 82-83 23.221776929470455 26.362110369621668 27.00862979971928 23.407482901188597 84-85 23.08659692840898 26.596412124894357 26.839589411986097 23.477401534710566 86-87 23.210827741429572 26.606394075076174 26.66904110573038 23.513737077763878 88-89 22.92348070138154 26.625794462739925 26.36131809022504 24.089406745653502 90-91 23.08853300370699 26.35135855790719 26.63033217301716 23.92977626536867 92-93 23.43826752389505 26.3317220847963 26.764552931395098 23.46545745991356 94-95 23.177457331309185 26.146783926546846 26.900936932614616 23.77482180952935 96-97 22.96115647363414 26.00523791809597 27.27334477521787 23.760260833052026 98-99 23.048406925807484 26.40625964854502 26.443497184729992 24.101836240917503 100-101 23.286501367618616 25.390039291558402 27.118602213519864 24.204857127303114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2331.0 1 1285.5 2 313.0 3 396.5 4 357.5 5 395.5 6 636.5 7 1247.5 8 2070.5 9 2522.5 10 2524.5 11 2490.5 12 2657.0 13 2940.0 14 3268.5 15 3547.0 16 3741.0 17 3836.0 18 4037.5 19 4346.0 20 4630.5 21 5093.5 22 5796.5 23 6954.5 24 8520.5 25 10639.5 26 13554.0 27 16763.5 28 19675.0 29 22626.5 30 26176.5 31 30876.5 32 36665.5 33 39352.5 34 40698.0 35 43015.0 36 46579.5 37 51742.5 38 55899.5 39 58768.0 40 62382.5 41 68820.0 42 73707.5 43 77260.0 44 82545.5 45 89606.0 46 95476.0 47 105986.0 48 125132.0 49 124368.5 50 107472.0 51 100165.0 52 100117.5 53 102567.5 54 102990.0 55 97348.0 56 88980.5 57 82180.0 58 76089.5 59 67500.0 60 56166.0 61 46580.0 62 38333.0 63 29481.0 64 22009.5 65 16866.0 66 13074.5 67 10707.0 68 8673.0 69 6752.5 70 4999.5 71 3483.0 72 2624.5 73 2213.5 74 1776.5 75 1448.0 76 1063.0 77 649.0 78 421.0 79 278.0 80 163.5 81 122.0 82 101.5 83 72.5 84 47.0 85 29.5 86 15.5 87 15.0 88 16.5 89 14.5 90 13.0 91 8.5 92 6.0 93 6.0 94 4.0 95 6.0 96 5.5 97 2.5 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39798998865172097 2 0.12010789239494096 3 0.12260161064423164 4 0.13380473292835846 5 0.13618679215156151 6 0.07533262293379625 7 0.08530749593095899 8 0.0822554825512301 9 0.08203216449905482 10-11 0.0795570560874454 12-13 0.0840234171309511 14-15 0.07726804605264871 16-17 0.07875683306715062 18-19 0.0887130962266321 20-21 0.08718708953676764 22-23 0.09723640188465549 24-25 0.11013301939777821 26-27 0.11102629160647935 28-29 0.10523863208760321 30-31 0.09500322136290264 32-33 0.09409133931652021 34-35 0.34528692833835367 36-37 0.5407274436670909 38-39 0.5210568452379845 40-41 0.49237908537114156 42-43 0.521094064913347 44-45 0.5308270100206531 46-47 0.5283891212844063 48-49 0.535572518629378 50-51 0.5342884398293701 52-53 0.5327996528148683 54-55 0.5260070620612033 56-57 0.49721764316827277 58-59 0.4876335767624167 60-61 0.4348188574229618 62-63 0.5123288313654671 64-65 0.5390711681134575 66-67 0.5500695821830903 68-69 0.565478527783185 70-71 0.5599886256672092 72-73 0.546589542536692 74-75 0.5507209265019348 76-77 0.5252254488785898 78-79 0.4983714531045117 80-81 0.5053501422349894 82-83 0.5202380123800084 84-85 0.5137803987046065 86-87 0.5150830873422956 88-89 0.5252254488785898 90-91 0.5446913390932021 92-93 0.5510931232555604 94-95 0.5464034441598794 96-97 0.5470175688033614 98-99 0.5477247426352497 100-101 0.5225456322524863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2686751.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.386416820644335 #Duplication Level Percentage of deduplicated Percentage of total 1 72.05029294695575 35.58305799527887 2 14.436698564044395 14.25953625595788 3 5.336867736323777 7.907063236282038 4 2.669745237130445 5.273966043434164 5 1.4821908610533765 3.6600047835865888 6 0.9094684888658545 2.694923392582237 7 0.5891984245643782 2.036887928992235 8 0.4460742099596595 1.7624005492805888 9 0.3179475339024665 1.4132060492762841 >10 1.6260438988966373 14.086664472387772 >50 0.0882959179276914 2.981210012241765 >100 0.04090448235172377 3.608451885683823 >500 0.0035514434593508573 1.241894867367392 >1k 0.002342441430613639 2.121397804163244 >5k 3.022505071759534E-4 0.9457418078510579 >10k+ 7.556262679398835E-5 0.4235929156341932 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11351 0.42248053504027727 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7460 0.2776587782046047 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6725 0.25030231681313225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5780 0.21512972359552487 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5378 0.20016741409978073 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4375 0.16283607971114555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3771 0.14035539579216683 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3326 0.12379264025583316 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2905 0.10812315692820065 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2707 0.10075366120641623 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.7219675362547554E-5 2 0.0 0.0 0.0 0.0 3.7219675362547554E-5 3 0.0 0.0 0.0 0.0 3.7219675362547554E-5 4 0.0 0.0 0.0 0.0 3.7219675362547554E-5 5 0.0 0.0 0.0 0.0 3.7219675362547554E-5 6 0.0 0.0 0.0 0.0 3.7219675362547554E-5 7 0.0 0.0 0.0 0.0 3.7219675362547554E-5 8 0.0 0.0 0.0 0.0 7.443935072509511E-5 9 0.0 0.0 0.0 0.0 7.443935072509511E-5 10-11 0.0 0.0 0.0 0.0 1.4887870145019022E-4 12-13 0.0 0.0 0.0 0.0 5.582951304382133E-4 14-15 0.0 0.0 0.0 1.8609837681273777E-5 6.69954156525856E-4 16-17 0.0 0.0 0.0 5.582951304382133E-5 7.443935072509511E-4 18-19 0.0 0.0 0.0 7.443935072509511E-5 7.443935072509511E-4 20-21 0.0 0.0 0.0 1.1165902608764266E-4 9.491017217449626E-4 22-23 0.0 0.0 0.0 2.6053772753783285E-4 0.0010235410724700575 24-25 0.0 0.0 0.0 3.34977078262928E-4 0.0010421509101513314 26-27 0.0 0.0 0.0 7.816131826134986E-4 0.0011165902608764266 28-29 0.0 0.0 0.0 0.0027170363014659715 0.0011352000985577005 30-31 0.0 0.0 0.0 0.007778912150772439 0.0011538099362389741 32-33 0.0 0.0 0.0 0.0190192541102618 0.0011724197739202478 34-35 0.0 0.0 0.0 0.034372370197312666 0.0011910296116015217 36-37 0.0 0.0 0.0 0.06057502165254615 0.0011910296116015217 38-39 0.0 0.0 0.0 0.10000926769916527 0.0011910296116015217 40-41 0.0 0.0 0.0 0.14260718615160095 0.0011910296116015217 42-43 0.0 0.0 0.0 0.1900250525634865 0.0012096394492827956 44-45 0.0 0.0 0.0 0.24849716255804877 0.001265468962326617 46-47 0.0 0.0 0.0 0.3203497458454468 0.0013026886376891643 48-49 0.0 0.0 0.0 0.3959056868314183 0.0013212984753704382 50-51 0.0 0.0 0.0 0.48097125487252074 0.001414347663776807 52-53 0.0 0.0 0.0 0.5668184360962367 0.0014701771768206285 54-55 0.0 0.0 0.0 0.6663810676910513 0.0015260066898644497 56-57 0.0 0.0 0.0 0.7902295374599284 0.0015260066898644497 58-59 0.0 0.0 0.0 0.9183582698954983 0.0015260066898644497 60-61 0.0 0.0 0.0 1.061895947931163 0.0015260066898644497 62-63 0.0 0.0 0.0 1.2077226360016242 0.0015632263652269973 64-65 0.0 0.0 0.0 1.3634497577185232 0.001581836202908271 66-67 0.0 0.0 0.0 1.5541075447631731 0.0016004460405895447 68-69 0.0 0.0 0.0 1.7678415305326025 0.0016004460405895447 70-71 0.0 0.0 0.0 2.002939609960134 0.0016004460405895447 72-73 0.0 0.0 0.0 2.2473239983906215 0.0016934952289959135 74-75 0.0 0.0 0.0 2.507712847227004 0.001749324742039735 76-77 0.0 0.0 0.0 2.7960908919360223 0.001749324742039735 78-79 0.0 0.0 0.0 3.135459891891731 0.00182376409276483 80-81 0.0 0.0 0.0 3.497439844630187 0.001916813281171199 82-83 0.0 0.0 0.0 3.8670870504933283 0.0021215214956652106 84-85 0.0 0.0 0.0 4.252012933092795 0.002158741171027758 86-87 0.0 0.0 0.0 4.663681152440253 0.002177351008709032 88-89 0.0 0.0 0.0 5.1096286927966155 0.0021959608463903058 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9605 0.0 23.283262 1 TACATGG 9615 0.0 22.956385 2 GAGTACT 7655 0.0 21.55291 12-13 ACATGGG 9850 0.0 21.351566 3 AGTACTT 7960 0.0 19.924164 12-13 AGAGTAC 13300 0.0 19.737043 10-11 GTACTTT 8280 0.0 19.668554 14-15 TAAGGTG 1300 0.0 19.31112 5 CATGGGG 6180 0.0 19.161722 4 GTAAGGT 1290 0.0 17.992413 4 GTAGGAC 505 0.0 17.821539 3 GTATCAA 21985 0.0 17.25602 1 TACTTTT 9185 0.0 17.112097 14-15 GTGTAAG 1045 0.0 16.811472 1 ACTTTTT 9945 0.0 16.423996 16-17 GTATTGG 965 0.0 16.237041 1 TAGGACT 855 0.0 16.066263 4 GAGTACA 7765 0.0 15.898353 1 GGACCGA 390 5.7752914E-9 15.7804365 6 GTATTAG 725 0.0 15.717865 1 >>END_MODULE