FastQCFastQC Report
Fri 27 May 2016
SRR939086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686751
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124780.46442710917386837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76230.2837255852887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63410.23600996147391404No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61780.2299431543898188No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47950.1784683433634155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41650.15501994788501056No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39110.14556615034292347No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36700.13659620858054952No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31820.11843300700362631No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28590.10641105186152346No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28380.10562943867890996No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28010.10425231069049569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG3300.024.44536694-95
GTACATG92950.023.2754691
TAGCGCG1452.3542176E-522.9078064
TACATGG95250.022.766942
ACATGGG98300.021.1916353
GTAAGGT12000.020.9579144
GAGTACT84650.020.93907512-13
CGTAAAC1856.615219E-620.5197333
AGAGTAC135650.019.76598510-11
AGTACTT87650.019.70803612-13
TAAGGTG13250.019.6970085
GTACTTT90700.019.43772114-15
TACTTTT100400.017.27617314-15
GTATAGG6800.016.8187731
CATGGGG62850.016.6100464
CATGGGA43850.016.1239014
ACTTTTT109850.016.11322416-17
TAAGGTA8050.015.915544
AGGTAAG13500.015.8172952
GTATCAA226850.015.796871
Produced by FastQC (version 0.11.5)