##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2686751 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53677099217605 34.0 31.0 34.0 31.0 34.0 2 32.758804221157824 34.0 31.0 34.0 31.0 34.0 3 32.86446194679001 34.0 31.0 34.0 31.0 34.0 4 36.29433728693132 37.0 37.0 37.0 35.0 37.0 5 36.255463941392414 37.0 37.0 37.0 35.0 37.0 6 36.2951873843166 37.0 37.0 37.0 35.0 37.0 7 36.252129802873434 37.0 37.0 37.0 35.0 37.0 8 36.22732772780209 37.0 37.0 37.0 35.0 37.0 9 37.98288118251375 39.0 38.0 39.0 35.0 39.0 10-11 37.90281440297221 39.0 38.0 39.0 35.0 39.0 12-13 37.824620331396545 39.0 38.0 39.0 35.0 39.0 14-15 39.25555568789218 40.5 39.0 41.0 36.0 41.0 16-17 39.35233056580233 41.0 39.0 41.0 36.0 41.0 18-19 39.377370474599246 41.0 39.0 41.0 36.0 41.0 20-21 39.33644092809494 41.0 39.0 41.0 36.0 41.0 22-23 39.26953093159731 41.0 39.0 41.0 36.0 41.0 24-25 39.19274878840652 41.0 39.0 41.0 36.0 41.0 26-27 39.04938194868077 40.5 39.0 41.0 35.0 41.0 28-29 38.9029212234405 40.0 38.0 41.0 35.0 41.0 30-31 38.7259881916858 40.0 38.0 41.0 35.0 41.0 32-33 38.63105568770608 40.0 38.0 41.0 35.0 41.0 34-35 38.518820314945444 40.0 38.0 41.0 34.0 41.0 36-37 38.37370601146142 40.0 38.0 41.0 34.0 41.0 38-39 38.2331367886343 40.0 38.0 41.0 34.0 41.0 40-41 38.05919435779497 40.0 38.0 41.0 33.0 41.0 42-43 37.87235512334414 40.0 38.0 41.0 33.0 41.0 44-45 37.621722481912165 40.0 37.0 41.0 33.0 41.0 46-47 37.32281331615769 40.0 37.0 41.0 32.5 41.0 48-49 36.97117540851386 40.0 36.5 41.0 31.0 41.0 50-51 36.69542190549106 39.5 36.0 41.0 31.0 41.0 52-53 36.42465155870417 39.0 35.5 41.0 30.5 41.0 54-55 36.16006916904469 39.0 35.0 40.0 30.0 41.0 56-57 35.88308853332519 39.0 35.0 40.0 29.5 41.0 58-59 35.51780235682428 38.0 35.0 40.0 28.5 41.0 60-61 35.104312978761335 38.0 34.5 40.0 28.0 41.0 62-63 34.688520633285336 37.0 34.0 40.0 27.0 41.0 64-65 34.22330744456781 36.5 34.0 39.5 26.0 41.0 66-67 33.770903034929546 36.0 33.0 39.0 26.0 40.0 68-69 33.2584809682773 35.5 33.0 38.5 25.5 40.0 70-71 32.72825449771862 35.0 32.0 38.0 24.5 40.0 72-73 32.14954679462295 35.0 31.5 37.0 22.5 39.0 74-75 31.621647484266312 35.0 31.0 36.5 20.5 39.0 76-77 29.661026086898264 32.5 28.5 35.0 18.0 36.5 78-79 30.646560473970233 34.0 30.5 35.0 19.0 37.0 80-81 30.66079588320615 34.0 31.0 35.0 18.5 37.0 82-83 30.294020919690734 34.0 30.5 35.0 17.0 36.5 84-85 29.835983870481485 34.0 30.0 35.0 9.5 36.0 86-87 29.158580754226946 34.0 29.0 35.0 4.5 35.5 88-89 28.488450734735 33.5 29.0 35.0 2.0 35.0 90-91 27.923045901909035 33.0 27.0 35.0 2.0 35.0 92-93 27.476477351269246 33.0 27.0 35.0 2.0 35.0 94-95 26.94030615416166 32.5 25.5 34.0 2.0 35.0 96-97 26.130515444118195 32.0 24.5 34.0 2.0 35.0 98-99 24.902549212785257 32.0 12.5 34.0 2.0 35.0 100-101 18.956021045493237 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18015570409615833 1101 2 0.13707352051915223 1101 3 0.13934510639347053 1101 4 0.11585762465308846 1101 5 0.15781204529596238 1101 6 0.11340521510438606 1101 7 0.1445837497035214 1101 8 0.16946608597154977 1101 9 0.18766284342382278 1101 10-11 0.17436429212874316 1101 12-13 0.22007715577685616 1101 14-15 0.2189927261533242 1101 16-17 0.23880903420002397 1101 18-19 0.27844508749250707 1101 20-21 0.2728647329210716 1101 22-23 0.30009901824514174 1101 24-25 0.25576354149058744 1101 26-27 0.25576084580281844 1101 28-29 0.2698266866252297 1101 30-31 0.13992520088516613 1101 32-33 0.3045878270860385 1101 34-35 0.44257622935683116 1101 36-37 0.41249405506268744 1101 38-39 0.21859191822591129 1101 40-41 0.420061840111309 1101 42-43 0.3857455568724859 1101 44-45 0.45047563526919276 1101 46-47 0.45579320398740464 1101 48-49 0.44747298049625783 1101 50-51 0.488111722140836 1101 52-53 0.3594275313319599 1101 54-55 0.3890494732443628 1101 56-57 0.4377527587945522 1101 58-59 0.24956436850050778 1101 60-61 0.34525113699638865 1101 62-63 0.28619288311271873 1101 64-65 0.3615439307651229 1101 66-67 0.2012832162305429 1101 68-69 0.22091399017934776 1101 70-71 0.1804178041197133 1101 72-73 0.2681639536474236 1101 74-75 -0.007939271816034932 1101 76-77 0.2070114904711282 1101 78-79 0.3646275654360558 1101 80-81 0.4117959209533346 1101 82-83 0.22722197117127862 1101 84-85 0.09549552972178432 1101 86-87 0.3561569953081438 1101 88-89 0.47005577978494273 1101 90-91 0.5480752948491272 1101 92-93 0.48794226282985775 1101 94-95 0.289295398411646 1101 96-97 0.031706382634226316 1101 98-99 0.02379277329228202 1101 100-101 -0.841471300844578 1102 1 0.07415252298095254 1102 2 0.07400218899940825 1102 3 0.0645276390189764 1102 4 0.0667946450280752 1102 5 0.10105819142183492 1102 6 0.08397329654563634 1102 7 0.18311698854247282 1102 8 0.14992416362714778 1102 9 0.14596939845799284 1102 10-11 0.2004808371558937 1102 12-13 0.20451602935687418 1102 14-15 0.26662343359929963 1102 16-17 0.26442027639030385 1102 18-19 0.21442862874283009 1102 20-21 0.32841539746307546 1102 22-23 0.21462690582617228 1102 24-25 0.2292913586410208 1102 26-27 0.23891876293535574 1102 28-29 0.200984998462566 1102 30-31 0.14655393040526832 1102 32-33 0.2083189245414303 1102 34-35 0.1945644263629447 1102 36-37 0.30002263080933034 1102 38-39 0.20887909897722778 1102 40-41 0.32181281073294343 1102 42-43 0.31718008869977865 1102 44-45 0.3913739114803789 1102 46-47 0.3218129362924529 1102 48-49 0.2296300495270387 1102 50-51 0.14486766692324693 1102 52-53 0.09396012895902572 1102 54-55 0.09808399450707128 1102 56-57 0.08044404388879656 1102 58-59 -0.03138272491687388 1102 60-61 -0.05504170021209376 1102 62-63 -0.14314743898987103 1102 64-65 -0.02230849806328905 1102 66-67 0.0540181609571988 1102 68-69 0.09116047525245818 1102 70-71 0.1013165425316842 1102 72-73 0.037753196547875234 1102 74-75 0.07309149461556785 1102 76-77 0.02861904679359384 1102 78-79 0.1296379025931813 1102 80-81 0.25388300691680854 1102 82-83 0.21329395417403063 1102 84-85 0.20250253022947007 1102 86-87 0.27557687463705705 1102 88-89 0.25634184245056346 1102 90-91 0.40647183230499806 1102 92-93 0.47655910131782875 1102 94-95 0.4579152301999052 1102 96-97 0.2200335753000573 1102 98-99 -0.02644258227951113 1102 100-101 -0.7321166138352098 1103 1 0.056306820430563675 1103 2 0.10577914295011226 1103 3 0.12051785521750702 1103 4 0.11440513691677978 1103 5 0.12029508640345199 1103 6 0.11149184876062179 1103 7 0.049092222668669194 1103 8 0.08443146246616351 1103 9 0.2518027431251113 1103 10-11 0.14606130477121582 1103 12-13 0.11868926305787397 1103 14-15 0.12708651062920495 1103 16-17 0.17477265215470794 1103 18-19 0.1887330862135741 1103 20-21 0.1876288350863362 1103 22-23 0.1540928554340084 1103 24-25 0.20319871603395256 1103 26-27 0.1629647384987578 1103 28-29 0.19132771784111213 1103 30-31 0.18678845388159715 1103 32-33 0.15736645700915375 1103 34-35 0.20961217500772023 1103 36-37 0.26800033021297764 1103 38-39 0.39044706984056887 1103 40-41 0.40815442918329836 1103 42-43 0.3036398529236166 1103 44-45 0.32186403927537555 1103 46-47 0.33855144723337816 1103 48-49 0.0712631741516887 1103 50-51 0.24568867446582487 1103 52-53 0.2640787686469892 1103 54-55 0.21683696067822922 1103 56-57 0.2625825217589224 1103 58-59 0.08213743216224145 1103 60-61 0.1940064407009956 1103 62-63 0.1457644316360387 1103 64-65 0.09094823796376517 1103 66-67 -0.0051560140445729985 1103 68-69 -0.1342889332312751 1103 70-71 0.08744094598679197 1103 72-73 -0.03311959327433556 1103 74-75 -0.1347835063686844 1103 76-77 -0.09367453453729624 1103 78-79 -0.05466520913957851 1103 80-81 0.07628943696742496 1103 82-83 -0.0376842112271909 1103 84-85 -0.07947145264672884 1103 86-87 -0.06294494671908879 1103 88-89 0.1605106237631304 1103 90-91 0.2094078317266188 1103 92-93 0.2829295958693798 1103 94-95 0.2515946281682737 1103 96-97 0.04965670424108026 1103 98-99 -0.17656555982501843 1103 100-101 -0.6718717539458581 1104 1 0.045614404233084827 1104 2 0.0656823672364979 1104 3 0.14273764298921776 1104 4 0.08262885061255787 1104 5 0.09705202685178449 1104 6 0.11251649636817973 1104 7 0.10280779989198408 1104 8 0.08620731194512388 1104 9 0.18823675619754 1104 10-11 0.18158861305737162 1104 12-13 0.17418017382478723 1104 14-15 0.19925451568949626 1104 16-17 0.23046993706211794 1104 18-19 0.2680255565498655 1104 20-21 0.2938760038910999 1104 22-23 0.2817758482442798 1104 24-25 0.3123534385106055 1104 26-27 0.19252697423237208 1104 28-29 0.25372760488566826 1104 30-31 0.15361793827988635 1104 32-33 0.16227781759602777 1104 34-35 0.26006385920988606 1104 36-37 0.2771555170314528 1104 38-39 0.3361407466400905 1104 40-41 0.24637593467577545 1104 42-43 0.35547544771625184 1104 44-45 0.3386469863049868 1104 46-47 0.25438295788176646 1104 48-49 0.2883925029782617 1104 50-51 0.17485608207297787 1104 52-53 0.1867184762010794 1104 54-55 0.21800821991475772 1104 56-57 0.20077948592652461 1104 58-59 0.20402793279495057 1104 60-61 0.2420518024249887 1104 62-63 0.3466700873537576 1104 64-65 0.29217936289888513 1104 66-67 0.36883322560773735 1104 68-69 0.30951186275378717 1104 70-71 0.25456306891629765 1104 72-73 0.09411221407128068 1104 74-75 -0.0169412064802259 1104 76-77 0.003389758918476815 1104 78-79 0.238574128798426 1104 80-81 0.3461943947965871 1104 82-83 0.2927440907848542 1104 84-85 0.20126178435574005 1104 86-87 0.209626365005267 1104 88-89 0.2658537018540734 1104 90-91 0.4594197842289205 1104 92-93 0.1310523306982141 1104 94-95 0.17856010968557356 1104 96-97 -0.16848738343215786 1104 98-99 -0.23463330970368546 1104 100-101 -0.6484985067072309 1105 1 0.03793370010394881 1105 2 0.08550379672869468 1105 3 0.06954000204005695 1105 4 0.0985762458480508 1105 5 0.06589774561230399 1105 6 0.11345710277072385 1105 7 0.1214218267535756 1105 8 0.07369799528444787 1105 9 0.15877290517362752 1105 10-11 0.18175985165886743 1105 12-13 0.1520292672914607 1105 14-15 0.08887822156561498 1105 16-17 0.16236953452249736 1105 18-19 0.19976971693099443 1105 20-21 0.17117535408260665 1105 22-23 0.1512652544879458 1105 24-25 0.11611441703401226 1105 26-27 0.19956946319501156 1105 28-29 0.23128694833408048 1105 30-31 0.2589507191698246 1105 32-33 0.2947961195012425 1105 34-35 0.3659862032110226 1105 36-37 0.341855265654182 1105 38-39 0.39674665351522975 1105 40-41 0.3706150705886557 1105 42-43 0.3375040438141994 1105 44-45 0.29378280537855517 1105 46-47 0.41423988923612853 1105 48-49 0.4728319456402872 1105 50-51 0.3539121956070872 1105 52-53 0.2939857792421705 1105 54-55 0.2077181854690977 1105 56-57 0.15691529185988173 1105 58-59 0.13079794030816316 1105 60-61 -0.03999206293340052 1105 62-63 0.151555967928509 1105 64-65 0.15391604333838416 1105 66-67 0.20049071048111244 1105 68-69 0.01506247178603104 1105 70-71 0.038285471765632906 1105 72-73 -0.052177613457601524 1105 74-75 -0.07791745113702575 1105 76-77 -0.08284372204543189 1105 78-79 -0.11832063256449388 1105 80-81 0.20031424252313812 1105 82-83 0.2188515155209565 1105 84-85 0.15530921121090913 1105 86-87 0.2263900336130824 1105 88-89 0.16779427848322115 1105 90-91 0.2700682304652382 1105 92-93 0.19666449248118667 1105 94-95 0.17209587327085174 1105 96-97 -0.2715637069468677 1105 98-99 -0.17261458040887945 1105 100-101 -0.7520015544882064 1106 1 -0.05066695382722486 1106 2 -0.029980532571300955 1106 3 -0.04454517205851971 1106 4 0.02963089183590739 1106 5 0.0994572514403913 1106 6 0.08252331791194933 1106 7 0.09144221721654588 1106 8 0.03166656427379877 1106 9 0.09403051633529458 1106 10-11 0.12010482413028711 1106 12-13 0.11033880959463005 1106 14-15 0.047663881017392384 1106 16-17 0.0327809929080658 1106 18-19 0.03229512575120452 1106 20-21 0.061507385743297505 1106 22-23 0.10698328637962362 1106 24-25 0.021479603011663073 1106 26-27 0.06960589946098139 1106 28-29 0.10375532661777243 1106 30-31 0.12092389679848026 1106 32-33 0.10679980356584906 1106 34-35 0.03455067967949077 1106 36-37 -0.1429909938782714 1106 38-39 0.027734512083121388 1106 40-41 0.08615856878722639 1106 42-43 0.0938946245396366 1106 44-45 0.19628311998628334 1106 46-47 0.07473180632461407 1106 48-49 -0.05858361768871845 1106 50-51 -0.037660959505686264 1106 52-53 -0.009771785496660357 1106 54-55 -0.10694656238695899 1106 56-57 -0.10683816114192979 1106 58-59 -0.07668407852560222 1106 60-61 -0.18725589766680883 1106 62-63 -0.04987700962580277 1106 64-65 0.013041333561986335 1106 66-67 -0.12639513236512556 1106 68-69 -0.332162235127349 1106 70-71 -0.2558043349115593 1106 72-73 -0.33232956980008055 1106 74-75 -0.39458897575681817 1106 76-77 -0.43996514704214107 1106 78-79 -0.10141252940162815 1106 80-81 -0.010963854211698987 1106 82-83 -0.296271815561866 1106 84-85 -0.23899739173407752 1106 86-87 -0.30471173074588975 1106 88-89 -0.25788890554758126 1106 90-91 0.10325471989910895 1106 92-93 -0.15492533603253023 1106 94-95 -0.3934784872181716 1106 96-97 -0.8676754789049497 1106 98-99 -0.7515724669695807 1106 100-101 -1.1327908624191387 1107 1 0.03370499192280363 1107 2 0.04696848459771985 1107 3 -0.025270285388387492 1107 4 0.046476287827431406 1107 5 -0.015449312079049093 1107 6 -0.014239648489990486 1107 7 0.0394656441831458 1107 8 0.057679423883236325 1107 9 -0.02031812979547709 1107 10-11 0.08733034577531384 1107 12-13 0.09047617781948958 1107 14-15 0.07837591960734613 1107 16-17 0.0825156962351059 1107 18-19 0.0896284784545216 1107 20-21 0.062183995806336156 1107 22-23 0.08715156415509995 1107 24-25 0.04685974411994209 1107 26-27 0.027592865521675947 1107 28-29 0.009377495689307125 1107 30-31 0.09389828576302506 1107 32-33 0.07328071413648729 1107 34-35 0.11059976528225945 1107 36-37 0.16114816586414094 1107 38-39 0.09042866269795269 1107 40-41 0.1027495791771571 1107 42-43 0.04607523151666015 1107 44-45 0.1270075533060364 1107 46-47 0.12477699608762549 1107 48-49 0.021729131326345907 1107 50-51 -0.018359026978757242 1107 52-53 0.03675234280764528 1107 54-55 -0.29068529508301566 1107 56-57 -0.1441195610725785 1107 58-59 -0.1044921648349586 1107 60-61 -0.1592691541548774 1107 62-63 -0.29563235558713075 1107 64-65 -0.28345791255406994 1107 66-67 -0.3330276419177238 1107 68-69 -0.24204976763646613 1107 70-71 -0.2353122301380708 1107 72-73 -0.4328703014786015 1107 74-75 -0.49527992785134245 1107 76-77 -0.44619079417994456 1107 78-79 -0.25554037624851134 1107 80-81 -0.30263750128521494 1107 82-83 -0.43185547751523856 1107 84-85 -0.5936701121392645 1107 86-87 -0.633594449401599 1107 88-89 -0.8519145221467497 1107 90-91 -0.47495138869355813 1107 92-93 -0.3536113092166424 1107 94-95 -0.30781807685061224 1107 96-97 -0.5760777147782044 1107 98-99 -0.5419205579648221 1107 100-101 -1.1727315655372301 1108 1 -0.0420730240027396 1108 2 0.041088793230066756 1108 3 0.06684786201818582 1108 4 0.01710139389780352 1108 5 0.010064247480080724 1108 6 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0.5389358060947096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 15.0 5 60.0 6 86.0 7 249.0 8 598.0 9 1166.0 10 1986.0 11 3162.0 12 4715.0 13 6621.0 14 8347.0 15 11159.0 16 14606.0 17 20717.0 18 21509.0 19 15944.0 20 15246.0 21 16051.0 22 17000.0 23 18182.0 24 20951.0 25 24293.0 26 28172.0 27 33647.0 28 40667.0 29 50225.0 30 61009.0 31 75182.0 32 95411.0 33 125435.0 34 176482.0 35 270018.0 36 444963.0 37 657987.0 38 387661.0 39 17214.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02155234447865 18.775342368458926 14.960366189864734 23.24273909719769 2 15.728848709835782 20.330428833933624 39.17177289596245 24.768949560268144 3 19.932997140412343 23.688201846765853 29.405330080829966 26.973470931991837 4 13.69844097945809 16.57574520303519 35.99503638409365 33.730777433413074 5 14.450241202106188 36.83638714566404 33.496665675382644 15.216705976847129 6 32.73351345174897 35.215246965572916 16.795415727024945 15.255823855653167 7 29.148142124074766 32.174362268777415 20.215420037063353 18.462075570084462 8 26.44234616456828 34.292794531387536 19.332867094866625 19.931992209177555 9 26.172094101760823 17.487292272339342 20.31308446521468 36.027529160685155 10-11 25.700036959137634 25.9087648985708 26.44029908242334 21.95089905986822 12-13 26.78722367647765 23.20853328053102 27.066780658125744 22.937462384865587 14-15 23.198586829164437 24.81641210634215 25.754208470985485 26.23079259350793 16-17 22.68869028853709 27.89872374605044 26.398923434068156 23.01366253134432 18-19 22.7600404280189 26.792104439917292 28.223238443614324 22.224616688449487 20-21 23.558081861698383 25.536977561374314 28.15460010994692 22.75034046698038 22-23 23.129441470385608 25.44223487773895 28.362360337820665 23.065963314054784 24-25 23.405880710896064 25.630445705778353 28.21378989485438 22.7498836884712 26-27 22.911422016837594 25.678363875829024 28.649685400104552 22.76052870722883 28-29 22.645660130023217 26.710458096042395 28.161299651512177 22.48258212242221 30-31 22.92186734088868 25.76634381079601 27.964816985273288 23.346971863042015 32-33 22.836894822035983 25.896556845051887 28.07455919807976 23.19198913483237 34-35 23.621961658129646 25.861396686651627 27.748375961985328 22.768265693233396 36-37 23.47300812457256 25.384272587730155 28.30665039989963 22.83606888779765 38-39 23.049177276450003 25.928020996202818 28.15883031792123 22.863971409425954 40-41 23.127285721464425 26.09205606519971 27.874365116144894 22.90629309719097 42-43 23.018359865878136 26.08426837475507 27.305256779050307 23.592114980316484 44-45 23.175452877613896 25.820919188298603 27.647169172944924 23.35645876114258 46-47 23.763478882308934 26.332701806365645 27.209531714688772 22.694287596636652 48-49 23.565077927061626 26.195016226352312 27.126184408334602 23.11372143825146 50-51 22.99679443935256 26.61173635310484 27.449518235406323 22.94195097213628 52-53 23.5746632270724 26.647556844679688 26.642699677044877 23.135080251203032 54-55 23.022434903718285 26.203730825818987 27.02550403814868 23.748330232314046 56-57 23.33058403691005 26.326529320948595 26.906058923278252 23.4368277188631 58-59 23.764474765804835 26.011374914556008 26.87805102278761 23.34609929685155 60-61 23.175600568060535 26.24086444059046 27.608888349604 22.974646641745007 62-63 22.78703980097651 26.451771268886425 27.466894553131365 23.294294377005702 64-65 22.684969788013618 26.72311051326506 27.1142062960931 23.477713402628222 66-67 22.855877893053695 26.347064459145702 26.910656740216787 23.88640090758382 68-69 22.838072231755024 26.1827642809021 27.26220208960668 23.716961397736195 70-71 23.208524960775033 26.28181567625218 26.860768911681436 23.64889045129135 72-73 23.13472666428709 26.05423799972532 27.229188711570217 23.58184662441737 74-75 22.75869628409927 27.061551293737306 26.595188761444582 23.58456366071884 76-77 22.971602802482227 26.283730555315977 26.756026787918053 23.98863985428374 78-79 23.048481092057678 26.461775561598554 26.899533001650966 23.590210344692803 80-81 23.249939285212484 26.44743588192765 26.701554019411983 23.601070813447883 82-83 23.087774043817237 26.37118214527509 26.98610701177742 23.55493679913025 84-85 22.871211362720253 26.626341629723036 26.813072741016935 23.689374266539772 86-87 22.969055179192466 26.617028221146 26.59921814511412 23.81469845454742 88-89 22.68965624219155 26.626732249714202 26.291621789973153 24.391989718121103 90-91 22.871899918979004 26.323151352265644 26.53165659433339 24.27329213442196 92-93 23.103983212437623 26.407843525507204 26.630696331740456 23.857476930314718 94-95 22.7854014011719 26.166213393053546 26.83399019857069 24.21439500720387 96-97 22.54859121667769 26.03086404359764 27.171963460700304 24.248581279024368 98-99 22.55053977954737 26.48948820971101 26.319058935626565 24.640913075115055 100-101 22.829659245540327 25.067702765875723 27.20288944536584 24.899748543218113 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 381.0 1 263.5 2 206.5 3 293.0 4 266.0 5 253.0 6 466.0 7 1054.0 8 1809.0 9 2159.5 10 2048.5 11 1919.0 12 2049.5 13 2320.5 14 2575.0 15 2712.5 16 2861.5 17 3063.5 18 3241.0 19 3589.0 20 3934.5 21 4473.0 22 5455.0 23 6903.0 24 8570.0 25 10547.0 26 13279.0 27 16442.0 28 19472.0 29 22868.0 30 26596.0 31 31006.0 32 36645.5 33 39532.5 34 41126.5 35 43399.0 36 46753.5 37 51487.0 38 55826.5 39 59056.0 40 62659.5 41 68927.5 42 74123.5 43 78094.0 44 83107.5 45 89848.0 46 95516.5 47 106991.0 48 126290.5 49 124961.5 50 109073.0 51 102106.5 52 101367.5 53 103501.0 54 104041.5 55 98476.5 56 89930.0 57 82735.5 58 76446.0 59 68163.0 60 56594.5 61 46429.5 62 38172.5 63 29249.0 64 21662.5 65 16522.0 66 12688.5 67 10342.0 68 8154.0 69 6303.0 70 4646.5 71 3147.0 72 2397.0 73 2061.0 74 1699.5 75 1355.0 76 948.0 77 537.5 78 321.0 79 191.5 80 110.5 81 57.5 82 32.5 83 21.0 84 12.0 85 6.5 86 4.0 87 3.5 88 2.0 89 1.5 90 1.5 91 2.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4330881425186033 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010049312347887838 16-17 0.002233180521752853 18-19 1.8609837681273777E-5 20-21 0.0 22-23 0.0 24-25 3.7219675362547554E-5 26-27 0.003145062568135268 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011705587901521205 36-37 0.026128212104508382 38-39 0.020508041124763704 40-41 0.008783843385561223 42-43 0.008895502411648866 44-45 0.0036475281855296604 46-47 0.005247974226119205 48-49 0.0026798166261034237 50-51 5.024656173943919E-4 52-53 0.0 54-55 0.0 56-57 1.1165902608764265E-4 58-59 0.0018423739304461036 60-61 0.0024378887362468647 62-63 0.0012282492869640693 64-65 0.002568157600015781 66-67 0.00212152149566521 68-69 0.004503580718868254 70-71 2.0470821449401153E-4 72-73 0.0 74-75 0.0 76-77 0.09790635604118134 78-79 0.01659997521169621 80-81 3.1636724058165417E-4 82-83 0.0 84-85 0.0 86-87 0.10708100601804932 88-89 0.4229830006576717 90-91 0.42871483066350397 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.233180521752853E-4 100-101 2.605377275378329E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2686751.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.6921925147358 #Duplication Level Percentage of deduplicated Percentage of total 1 70.36124749076485 33.55682160906529 2 15.1232875692575 14.425254844174793 3 5.7898151744986235 8.28386939780781 4 2.83433723644107 5.40703028528127 5 1.5705205877697377 3.745078511013518 6 0.9881393373249291 2.827591890425039 7 0.6635427147129361 2.215206482428784 8 0.4644865899026498 1.772190709292025 9 0.3352847520947697 1.4391418449743305 >10 1.7234500091432494 14.542228309282567 >50 0.09561705839839295 3.1417244871258974 >100 0.04323835348163814 3.669058543450187 >500 0.004298021572644882 1.4447136453913512 >1k 0.002422521250014907 2.3143594043682203 >5k 2.3443754032402328E-4 0.7506816181323827 >10k+ 7.814584677467442E-5 0.4650484177865093 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12478 0.46442710917386837 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7623 0.2837255852887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6341 0.23600996147391404 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6178 0.2299431543898188 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4795 0.1784683433634155 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4165 0.15501994788501056 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3911 0.14556615034292347 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3670 0.13659620858054952 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3182 0.11843300700362631 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2859 0.10641105186152346 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2838 0.10562943867890996 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2801 0.10425231069049569 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.7219675362547554E-5 2 0.0 0.0 0.0 0.0 3.7219675362547554E-5 3 0.0 0.0 0.0 0.0 3.7219675362547554E-5 4 0.0 0.0 0.0 0.0 3.7219675362547554E-5 5 0.0 0.0 0.0 0.0 3.7219675362547554E-5 6 0.0 0.0 0.0 0.0 1.1165902608764266E-4 7 0.0 0.0 0.0 0.0 1.1165902608764266E-4 8 0.0 0.0 0.0 0.0 1.4887870145019022E-4 9 0.0 0.0 0.0 0.0 1.4887870145019022E-4 10-11 0.0 0.0 0.0 0.0 1.67488539131464E-4 12-13 0.0 0.0 0.0 0.0 6.69954156525856E-4 14-15 0.0 0.0 0.0 1.8609837681273777E-5 8.002230202947725E-4 16-17 0.0 0.0 0.0 7.443935072509511E-5 8.932722087011413E-4 18-19 0.0 0.0 0.0 1.1165902608764266E-4 9.304918840636888E-4 20-21 0.0 0.0 0.0 1.3026886376891643E-4 9.863213971075101E-4 22-23 0.0 0.0 0.0 2.6053772753783285E-4 0.0010049312347887838 24-25 0.0 0.0 0.0 3.163672405816542E-4 0.0010049312347887838 26-27 0.0 0.0 0.0 7.071738318884035E-4 0.0010979804231951527 28-29 0.0 0.0 0.0 0.002679816626103424 0.0011538099362389741 30-31 0.0 0.0 0.0 0.007760302313091164 0.0011538099362389741 32-33 0.0 0.0 0.0 0.019037863947943073 0.0011910296116015217 34-35 0.0 0.0 0.0 0.03463290792485049 0.0012282492869640693 36-37 0.0 0.0 0.0 0.06126358564675327 0.0012282492869640693 38-39 0.0 0.0 0.0 0.10080949071946005 0.0012282492869640693 40-41 0.0 0.0 0.0 0.14374238625015864 0.0012282492869640693 42-43 0.0 0.0 0.0 0.19121608217508806 0.0012282492869640693 44-45 0.0 0.0 0.0 0.2500045594102319 0.001246859124645343 46-47 0.0 0.0 0.0 0.3226015362048809 0.0012840788000078906 48-49 0.0 0.0 0.0 0.39871577232129063 0.0013399083130517118 50-51 0.0 0.0 0.0 0.48404187808993093 0.0013771279884142594 52-53 0.0 0.0 0.0 0.5703170855803161 0.0014515673391393546 54-55 0.0 0.0 0.0 0.670772989383832 0.0016190558782708184 56-57 0.0 0.0 0.0 0.7961660756802547 0.0016748853913146399 58-59 0.0 0.0 0.0 0.9258208148056891 0.0017307149043584613 60-61 0.0 0.0 0.0 1.0703634240761426 0.0017679345797210087 62-63 0.0 0.0 0.0 1.2173997515958868 0.0019726427942150203 64-65 0.0 0.0 0.0 1.3746714898403312 0.0020098624695775676 66-67 0.0 0.0 0.0 1.5673577491922401 0.0020098624695775676 68-69 0.0 0.0 0.0 1.783641282724004 0.0020098624695775676 70-71 0.0 0.0 0.0 2.0208608836472006 0.0020284723072588415 72-73 0.0 0.0 0.0 2.2669759869820463 0.0021959608463903053 74-75 0.0 0.0 0.0 2.5298399442300386 0.0023634493855217696 76-77 0.0 0.0 0.0 2.81972538579124 0.0023820592232030435 78-79 0.0 0.0 0.0 3.1597643399034743 0.0023820592232030435 80-81 0.0 0.0 0.0 3.5220234402071497 0.002400669060884317 82-83 0.0 0.0 0.0 3.8923778199021792 0.0026053772753783285 84-85 0.0 0.0 0.0 4.279090246919049 0.0026425969507408763 86-87 0.0 0.0 0.0 4.690274610486792 0.002754255976828519 88-89 0.0 0.0 0.0 5.135608026199674 0.002847305165234888 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 330 0.0 24.445366 94-95 GTACATG 9295 0.0 23.275469 1 TAGCGCG 145 2.3542176E-5 22.907806 4 TACATGG 9525 0.0 22.76694 2 ACATGGG 9830 0.0 21.191635 3 GTAAGGT 1200 0.0 20.957914 4 GAGTACT 8465 0.0 20.939075 12-13 CGTAAAC 185 6.615219E-6 20.519733 3 AGAGTAC 13565 0.0 19.765985 10-11 AGTACTT 8765 0.0 19.708036 12-13 TAAGGTG 1325 0.0 19.697008 5 GTACTTT 9070 0.0 19.437721 14-15 TACTTTT 10040 0.0 17.276173 14-15 GTATAGG 680 0.0 16.818773 1 CATGGGG 6285 0.0 16.610046 4 CATGGGA 4385 0.0 16.123901 4 ACTTTTT 10985 0.0 16.113224 16-17 TAAGGTA 805 0.0 15.91554 4 AGGTAAG 1350 0.0 15.817295 2 GTATCAA 22685 0.0 15.79687 1 >>END_MODULE