FastQCFastQC Report
Fri 27 May 2016
SRR939085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105406
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.2565300944330927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44090.20941329130818473No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32520.15445951992157333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27570.130948615136463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73800.025.9899041
GTATCAA100700.025.7891791
GTACATG75850.024.4111731
TACATGG74800.024.0024492
TATACGG1201.7431729E-423.6857975
ACATGGG78950.022.2607483
ATCAACG118000.021.798963
CAACGCA119050.021.7658625
TCAACGC119250.021.6101844
GAGTACT56950.021.53517512-13
TATCAAC119650.021.4983482
AACGCAG121300.021.392536
GTACCGT2108.8758316E-720.2939036
GTACTTT60750.019.8772814-15
TGTACCG2902.5247573E-919.6020375
AGAGTAC97000.019.23319410-11
ACGCAGA134250.019.187887
CGCAGAG135500.018.9055828
TACCTGG18700.018.4926532
AGTACTT59450.018.47900412-13
Produced by FastQC (version 0.11.5)