##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939085_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2105406 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01035952210643 33.0 31.0 34.0 30.0 34.0 2 32.11102134220193 34.0 31.0 34.0 30.0 34.0 3 32.173880002241845 34.0 31.0 34.0 30.0 34.0 4 35.60396427102421 37.0 35.0 37.0 33.0 37.0 5 35.64768125482686 37.0 35.0 37.0 35.0 37.0 6 35.72671256755229 37.0 36.0 37.0 35.0 37.0 7 35.648382307260455 37.0 36.0 37.0 33.0 37.0 8 35.63224907690013 37.0 36.0 37.0 33.0 37.0 9 37.30667529208143 39.0 38.0 39.0 34.0 39.0 10-11 37.21203938812752 39.0 37.0 39.0 34.0 39.0 12-13 37.11646399791774 39.0 37.0 39.0 34.0 39.0 14-15 38.42549655505874 40.0 38.0 41.0 34.0 41.0 16-17 38.34901463185723 40.0 38.0 41.0 33.5 41.0 18-19 38.26272082439206 40.0 38.0 41.0 33.5 41.0 20-21 38.152965983758 40.0 38.0 41.0 33.0 41.0 22-23 38.068757759785996 40.0 38.0 41.0 33.0 41.0 24-25 38.1051248547786 40.0 38.0 41.0 33.0 41.0 26-27 38.0169936344819 40.0 38.0 41.0 33.0 41.0 28-29 37.890114543228236 40.0 38.0 41.0 33.0 41.0 30-31 37.729168863392616 40.0 38.0 41.0 33.0 41.0 32-33 37.53008160896283 40.0 37.5 41.0 32.0 41.0 34-35 37.216631851528874 40.0 37.0 41.0 31.0 41.0 36-37 36.95964768790438 40.0 37.0 41.0 30.5 41.0 38-39 36.719439623521545 40.0 36.5 41.0 30.0 41.0 40-41 36.5655947118988 40.0 36.0 41.0 30.0 41.0 42-43 36.364678594057395 40.0 36.0 41.0 30.0 41.0 44-45 36.11218548821462 39.0 35.5 41.0 29.0 41.0 46-47 35.76396049028074 39.0 35.0 41.0 27.5 41.0 48-49 35.76729666392135 39.0 35.0 41.0 27.0 41.0 50-51 34.76632274250193 38.0 34.0 40.0 25.5 40.5 52-53 34.9267649564977 38.5 34.5 39.5 26.0 40.5 54-55 35.4183216918732 39.0 35.0 41.0 27.0 41.0 56-57 35.28245834770111 39.0 35.0 41.0 26.0 41.0 58-59 35.075473566618506 39.0 35.0 41.0 26.0 41.0 60-61 34.76317845584177 38.0 35.0 40.5 26.0 41.0 62-63 34.38511883218723 37.5 34.0 40.0 25.0 41.0 64-65 34.00314642401513 37.0 34.0 40.0 23.5 41.0 66-67 33.59459695659649 36.5 34.0 39.5 22.5 41.0 68-69 33.18386192496839 36.0 33.0 39.0 22.0 41.0 70-71 32.774650827441356 35.5 33.0 39.0 21.0 40.5 72-73 32.32764773160141 35.0 32.5 38.0 20.0 40.0 74-75 31.868830762332777 35.0 32.0 37.0 18.0 39.0 76-77 31.439783348199825 35.0 32.0 36.5 16.0 39.0 78-79 31.027118047540476 35.0 31.0 36.0 12.0 39.0 80-81 30.619430884114514 35.0 31.0 36.0 7.5 37.0 82-83 30.211748945334058 34.5 31.0 35.0 4.5 37.0 84-85 29.841824094735173 34.0 30.0 35.0 2.0 36.5 86-87 29.467574900043033 34.0 30.0 35.0 2.0 36.0 88-89 29.08042534314047 34.0 29.5 35.0 2.0 36.0 90-91 28.719445798102598 34.0 29.0 35.0 2.0 35.5 92-93 28.31886130276061 34.0 29.0 35.0 2.0 35.0 94-95 27.869679767227794 34.0 28.5 35.0 2.0 35.0 96-97 27.305104098686904 34.0 27.0 35.0 2.0 35.0 98-99 26.603690452102825 33.5 25.5 35.0 2.0 35.0 100-101 24.0645039484071 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16467683642114395 1101 2 0.1872666283324378 1101 3 0.17991612908218713 1101 4 0.0269160499048553 1101 5 0.12559097530109398 1101 6 0.2386037516940931 1101 7 0.07653176733618494 1101 8 0.22559940666406675 1101 9 0.25984371234694237 1101 10-11 0.325405420857372 1101 12-13 0.17981281073850397 1101 14-15 0.2791199645011275 1101 16-17 0.359897527225435 1101 18-19 0.4165805170844692 1101 20-21 0.36203190768946314 1101 22-23 0.3871189637835357 1101 24-25 0.3407015832312439 1101 26-27 0.2458999378219744 1101 28-29 0.3257042247299111 1101 30-31 0.39348398963336706 1101 32-33 0.2726722809054678 1101 34-35 0.14834220465677106 1101 36-37 0.22082007902545087 1101 38-39 0.3776757605840899 1101 40-41 0.07074368445888979 1101 42-43 0.00397910809866886 1101 44-45 -0.027425202503856383 1101 46-47 -0.031728800000820456 1101 48-49 0.06978309084899337 1101 50-51 -0.040730339898580326 1101 52-53 -0.049898986507763254 1101 54-55 -0.09119867332145049 1101 56-57 -0.08486841862539762 1101 58-59 -0.018954228375868354 1101 60-61 -0.11817210630214703 1101 62-63 -0.2015308385428085 1101 64-65 -0.30753419116528136 1101 66-67 -0.31788642506563747 1101 68-69 -0.2811780282969707 1101 70-71 -0.0829621012701196 1101 72-73 -0.26905964027378815 1101 74-75 -0.23448554335099203 1101 76-77 -0.5117237844244045 1101 78-79 -0.45271074205432527 1101 80-81 -0.452512656698417 1101 82-83 -0.3393516042210223 1101 84-85 -0.4783714766585838 1101 86-87 -0.497947676803971 1101 88-89 -0.5517177983787889 1101 90-91 -0.7563574607065426 1101 92-93 -0.8934592876019032 1101 94-95 -0.9506301951461609 1101 96-97 -0.9062779830309289 1101 98-99 -1.196842065094092 1101 100-101 -1.0634883265172128 1102 1 0.2431725338112969 1102 2 0.35270317959712827 1102 3 0.42395887674647526 1102 4 0.33189214975630676 1102 5 0.3963928511602717 1102 6 0.4678504195950026 1102 7 0.4413266079945686 1102 8 0.5383427996833845 1102 9 0.5846609443613318 1102 10-11 0.44405424246853187 1102 12-13 0.4050297634440554 1102 14-15 0.4707625658052521 1102 16-17 0.7165466374751688 1102 18-19 0.7734117028787821 1102 20-21 0.7529472272546442 1102 22-23 0.7927086392692289 1102 24-25 0.5740193670117932 1102 26-27 0.7503626108775663 1102 28-29 0.7770034627532354 1102 30-31 0.8764255957175138 1102 32-33 0.7197269283509513 1102 34-35 0.7313514512955237 1102 36-37 0.7999746887146628 1102 38-39 0.7624133400143691 1102 40-41 0.7086977617702956 1102 42-43 0.6835488908471135 1102 44-45 0.7761699512836486 1102 46-47 0.7850386229086297 1102 48-49 0.7660076108415836 1102 50-51 0.7227230602783905 1102 52-53 0.8178867620355632 1102 54-55 0.7794199607766785 1102 56-57 0.7412358790280322 1102 58-59 0.6638812520105546 1102 60-61 0.6253073588126696 1102 62-63 0.5168969850494562 1102 64-65 0.6045345456327951 1102 66-67 0.6212124394768637 1102 68-69 0.6669747577212561 1102 70-71 0.5780980769569126 1102 72-73 0.47757925100422227 1102 74-75 0.4255902024079887 1102 76-77 0.5454260700172 1102 78-79 0.40774985513597173 1102 80-81 0.4063541599242484 1102 82-83 0.26030275837608485 1102 84-85 0.27436483159575076 1102 86-87 0.27813876315993724 1102 88-89 0.4121872191217477 1102 90-91 0.40822731521225464 1102 92-93 0.10393465594447449 1102 94-95 0.06696888587526928 1102 96-97 -0.006095615932228071 1102 98-99 -0.054062038300266124 1102 100-101 -0.15322372658048167 1103 1 0.31447166282819694 1103 2 0.37350189405236023 1103 3 0.38470150141191795 1103 4 0.3890414069471362 1103 5 0.3460097708114134 1103 6 0.2985345558941148 1103 7 0.36183570586456426 1103 8 0.4002162148066404 1103 9 0.45624311694122355 1103 10-11 0.5027317273303993 1103 12-13 0.49898044963298815 1103 14-15 0.48929315515164973 1103 16-17 0.46327987425042494 1103 18-19 0.5554488439139362 1103 20-21 0.7322032879847526 1103 22-23 0.5905978630875524 1103 24-25 0.47307576354261727 1103 26-27 0.4811716922573481 1103 28-29 0.4949030419644913 1103 30-31 0.5649356933787857 1103 32-33 0.5707191087607981 1103 34-35 0.5131106803376682 1103 36-37 0.4714868932025382 1103 38-39 0.49117296459101567 1103 40-41 0.3416552988934569 1103 42-43 0.14079368749279553 1103 44-45 0.12424535307405193 1103 46-47 0.3563520223027581 1103 48-49 0.24269514781483537 1103 50-51 0.24781514024785878 1103 52-53 0.40938914158493134 1103 54-55 0.3437914703000686 1103 56-57 0.3630878914683464 1103 58-59 0.4032367037559226 1103 60-61 0.2708591524981898 1103 62-63 0.18183501413450642 1103 64-65 0.15277879913926284 1103 66-67 0.13460381913116493 1103 68-69 0.09743564189653853 1103 70-71 0.12036895235662115 1103 72-73 0.06149815028822303 1103 74-75 0.08911243709717098 1103 76-77 0.06831604339069486 1103 78-79 0.07355751236093155 1103 80-81 0.12576207476555368 1103 82-83 0.12924476702249166 1103 84-85 -0.032520207398416545 1103 86-87 -0.07227784536815918 1103 88-89 -0.08098822522032023 1103 90-91 -0.16193335482087434 1103 92-93 -0.2745280006404833 1103 94-95 -0.35249184377632403 1103 96-97 -0.570206655034788 1103 98-99 -0.637807386733872 1103 100-101 -0.8541478992733822 1104 1 0.2005103798290051 1104 2 0.2393230963655597 1104 3 0.26475779752640705 1104 4 0.21799402310580973 1104 5 0.19060871851892358 1104 6 0.16504987242554137 1104 7 0.15446812444994862 1104 8 0.16711271892530988 1104 9 0.2092765111214021 1104 10-11 0.25768256410234613 1104 12-13 0.2316045848663748 1104 14-15 0.2701620634365369 1104 16-17 0.2880045294942377 1104 18-19 0.3115309718150243 1104 20-21 0.35504999995419695 1104 22-23 0.34368686310289576 1104 24-25 0.42597762116100313 1104 26-27 0.3892379897524023 1104 28-29 0.33237102067320734 1104 30-31 0.22105578664319836 1104 32-33 0.28428002974683153 1104 34-35 0.13797454191364977 1104 36-37 0.1627115254370466 1104 38-39 0.2647038200772016 1104 40-41 0.21985167569393838 1104 42-43 0.1253621095142492 1104 44-45 0.05898135957801287 1104 46-47 0.1793049605917858 1104 48-49 0.29682646962306336 1104 50-51 0.30348808589986476 1104 52-53 0.16594871269136746 1104 54-55 0.33609485573670383 1104 56-57 0.2171628404643684 1104 58-59 0.18760494477078993 1104 60-61 0.24868201148179736 1104 62-63 0.2967607707573521 1104 64-65 0.21226937299147863 1104 66-67 0.1527083120826518 1104 68-69 0.11606934900333954 1104 70-71 0.09652363628539007 1104 72-73 0.045113603341562225 1104 74-75 0.06620091556812469 1104 76-77 0.03529768196043648 1104 78-79 0.1845160802614103 1104 80-81 0.22665215161665486 1104 82-83 0.09241069483461217 1104 84-85 0.17808976943888055 1104 86-87 -0.02839717165552713 1104 88-89 0.18648926400365085 1104 90-91 -0.0594038645538113 1104 92-93 -0.1499690124365891 1104 94-95 -0.2795274008317463 1104 96-97 -0.3255812466195174 1104 98-99 -0.36047040770302274 1104 100-101 -0.5034687411073762 1105 1 0.14924989395653654 1105 2 0.13617763323703969 1105 3 0.22378129554024184 1105 4 0.2757201370244644 1105 5 0.3376197028160135 1105 6 0.2722268753550665 1105 7 0.23614926044781726 1105 8 0.23683789597705385 1105 9 0.2486599758359489 1105 10-11 0.2600249910342569 1105 12-13 0.24736864293343075 1105 14-15 0.3987048510933633 1105 16-17 0.30338064987852675 1105 18-19 0.35216861257226384 1105 20-21 0.29975291083706423 1105 22-23 0.3135145813801614 1105 24-25 0.16787286552278857 1105 26-27 0.2667704256530925 1105 28-29 0.38810239745729547 1105 30-31 0.1932212579043906 1105 32-33 0.39016416673089793 1105 34-35 0.30254230299389917 1105 36-37 0.21801965026470072 1105 38-39 0.3010298736499948 1105 40-41 0.26793426643214246 1105 42-43 0.19919205155209596 1105 44-45 0.18656481941088998 1105 46-47 0.27489342881958123 1105 48-49 0.24844461638191007 1105 50-51 0.22829932331720926 1105 52-53 0.13483696742361673 1105 54-55 0.13214999170445196 1105 56-57 0.18177391346261373 1105 58-59 0.17931193289428649 1105 60-61 0.1538509824913703 1105 62-63 0.13936037908051446 1105 64-65 0.052119531412351705 1105 66-67 -0.0023930165130323644 1105 68-69 0.059425124211372804 1105 70-71 -0.10285711753933668 1105 72-73 -0.1356908038797897 1105 74-75 -0.0969183111323737 1105 76-77 -0.035817605121238216 1105 78-79 -0.00256453028083925 1105 80-81 -0.0900019819450435 1105 82-83 -0.19368490713613085 1105 84-85 -0.13967936038106288 1105 86-87 -0.023044073061708303 1105 88-89 -0.025634489675137928 1105 90-91 0.029532796699729857 1105 92-93 -0.029091239811695146 1105 94-95 -0.06458058256990284 1105 96-97 0.09201977687122564 1105 98-99 0.03222704193129644 1105 100-101 0.23760427790468697 1106 1 0.17551822641743797 1106 2 0.3155155213819185 1106 3 0.2629982128930024 1106 4 0.2665018378976072 1106 5 0.2947370497290578 1106 6 0.16078553368267734 1106 7 0.22379196392413547 1106 8 0.24322103671931927 1106 9 0.32966471723742785 1106 10-11 0.24607499814484157 1106 12-13 0.28668276355524114 1106 14-15 0.3557393672051532 1106 16-17 0.4502845429514011 1106 18-19 0.4004587175568375 1106 20-21 0.4306395710689017 1106 22-23 0.4335792776571381 1106 24-25 0.32716343042633866 1106 26-27 0.38587241873615596 1106 28-29 0.32766768325759443 1106 30-31 0.3840948164971536 1106 32-33 0.41276688953188057 1106 34-35 0.4399148545053251 1106 36-37 0.318027819877976 1106 38-39 0.4553464300268857 1106 40-41 0.5119873426949795 1106 42-43 0.466956926047672 1106 44-45 0.4416143464306259 1106 46-47 0.5125375191129748 1106 48-49 0.6473320360852028 1106 50-51 0.47854983290944375 1106 52-53 0.4892858513636682 1106 54-55 0.4447457451192989 1106 56-57 0.5656172676839262 1106 58-59 0.5716264700655174 1106 60-61 0.5818581566221397 1106 62-63 0.6749176122644727 1106 64-65 0.5584131594165811 1106 66-67 0.5154322475621527 1106 68-69 0.49954879299306043 1106 70-71 0.5566137335830845 1106 72-73 0.4526002743982147 1106 74-75 0.3459346136396064 1106 76-77 0.36387152092177644 1106 78-79 0.5663964613286687 1106 80-81 0.5679741325263663 1106 82-83 0.6338788889704929 1106 84-85 0.7415132821257373 1106 86-87 0.602302631205351 1106 88-89 0.7801354762041193 1106 90-91 0.8055378860662223 1106 92-93 0.7751594049321646 1106 94-95 0.8095524046138891 1106 96-97 0.9340295420805766 1106 98-99 0.848771693910809 1106 100-101 0.5547911522610782 1107 1 0.030419919562362452 1107 2 0.13652118102397992 1107 3 0.11167103573097137 1107 4 0.00809941578445006 1107 5 0.0898984612964071 1107 6 0.10200270291210245 1107 7 0.0688985792366239 1107 8 0.09974930822388473 1107 9 0.07280282160352414 1107 10-11 0.08711566210928368 1107 12-13 0.2069054994511461 1107 14-15 0.1703002850127504 1107 16-17 0.18237808629088903 1107 18-19 0.07698846282349336 1107 20-21 0.07228170800414802 1107 22-23 0.18258844465515267 1107 24-25 0.1519464708865499 1107 26-27 0.06424387206340043 1107 28-29 0.007590295911377609 1107 30-31 0.03617296940837633 1107 32-33 0.17497166729053504 1107 34-35 0.2633151302669887 1107 36-37 0.27515024499799523 1107 38-39 0.42058913589364266 1107 40-41 0.2854310400376576 1107 42-43 0.33297662104815373 1107 44-45 0.3593329231523015 1107 46-47 0.4495576809696402 1107 48-49 0.5764712798968858 1107 50-51 0.36652685211186764 1107 52-53 0.4216616844348877 1107 54-55 0.5543548223068484 1107 56-57 0.601281568418436 1107 58-59 0.5386327449290746 1107 60-61 0.43687038192376804 1107 62-63 0.45664503947151047 1107 64-65 0.3845156620426451 1107 66-67 0.41677747583701574 1107 68-69 0.363484697200505 1107 70-71 0.4401677355889788 1107 72-73 0.33027505368009 1107 74-75 0.3491988977572049 1107 76-77 0.3812211131149894 1107 78-79 0.3434456830042443 1107 80-81 0.21999368113974427 1107 82-83 0.3076149154309533 1107 84-85 0.3778031404665363 1107 86-87 0.23715606606011974 1107 88-89 0.30379578690086717 1107 90-91 0.23321869887073987 1107 92-93 0.27485352511598293 1107 94-95 0.17473037374994504 1107 96-97 0.19371680778702327 1107 98-99 0.24298747444093038 1107 100-101 0.20947855644181246 1108 1 0.08388760145713547 1108 2 0.08212454056631202 1108 3 0.037399747336174016 1108 4 -0.014250543549721328 1108 5 0.08411923040146263 1108 6 0.12697706575834644 1108 7 0.2079413122862377 1108 8 0.1325027433157686 1108 9 0.3495603165883878 1108 10-11 0.2646865214819698 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-1.2593097731693632 2316 60-61 -1.1347566723419291 2316 62-63 -1.3112244925628005 2316 64-65 -1.3833147285456064 2316 66-67 -1.4079774043939253 2316 68-69 -1.3306536684715198 2316 70-71 -1.132753249998288 2316 72-73 -0.9770446230926808 2316 74-75 -0.952262577816839 2316 76-77 -1.1750471206682818 2316 78-79 -0.9337759194522128 2316 80-81 -0.9168916896929176 2316 82-83 -0.9965288338404505 2316 84-85 -0.9776661739601877 2316 86-87 -0.8022564463000883 2316 88-89 -0.9748247214059909 2316 90-91 -1.1250237162715457 2316 92-93 -1.2788825872160068 2316 94-95 -0.9671522751016717 2316 96-97 -0.8966038238483982 2316 98-99 -0.9504398373471901 2316 100-101 -0.7868214922633463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8641.0 3 2381.0 4 2359.0 5 2591.0 6 2865.0 7 3301.0 8 4168.0 9 4845.0 10 5776.0 11 6987.0 12 8698.0 13 12371.0 14 10851.0 15 10648.0 16 12570.0 17 16033.0 18 15287.0 19 11502.0 20 11593.0 21 12212.0 22 13263.0 23 14070.0 24 16119.0 25 18311.0 26 20711.0 27 24474.0 28 28721.0 29 34237.0 30 41189.0 31 50246.0 32 63401.0 33 83053.0 34 115111.0 35 172422.0 36 283139.0 37 457413.0 38 429570.0 39 74237.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.859027855558125 18.1388798200315 14.24968245759945 23.752409866810922 2 15.60279876715762 19.520321402636412 40.39812952644186 24.47875030376411 3 19.894995675145662 23.308325736901445 28.813658455862946 27.98302013208994 4 13.404319064852551 15.88847092168901 35.769389899989065 34.93782011346938 5 14.744592553836641 36.33489898569509 33.456651247963656 15.463857212504609 6 33.52601265988272 34.361907206815324 17.180739673733477 14.93134045956848 7 29.75685269196428 31.628833081193953 20.025492860382613 18.588821366459154 8 26.375748204760267 35.03764024456055 19.355591786044222 19.231019764634954 9 26.472979256063113 17.252780896980116 20.10090051714843 36.17333932980834 10-11 25.72136240572193 25.812045248718196 26.545851289645515 21.920741055914352 12-13 26.495623284922914 23.185322458262892 27.163825874629886 23.155228382184305 14-15 23.30010426081082 24.651334828388034 25.409876203955633 26.63868470684551 16-17 23.104878915474362 27.794927011766774 26.049627007131846 23.050567065627018 18-19 23.039721312999173 26.500487450265453 28.01604057259776 22.443750664137614 20-21 23.67829815642171 25.61295697176147 28.04350658366475 22.665238288152068 22-23 23.38390362518305 25.474121748649313 28.00025868859623 23.1417159375714 24-25 23.340315072376093 25.684666997681 28.000150288115634 22.974867641827277 26-27 23.044661551686623 25.896946587575904 28.045618678356693 23.012773182380784 28-29 22.90349841374617 26.300259707155334 27.995513500634644 22.80072837846385 30-31 23.03287302538351 25.779646590012078 27.87257363500623 23.314906749598183 32-33 22.910373865215476 25.93153669765667 27.968983844068095 23.18910559305976 34-35 23.576033106493078 25.964778004493596 27.613382995296952 22.845805893716374 36-37 23.438295089479638 25.638219667858454 27.797617058652246 23.12586818400966 38-39 23.087045616365923 25.968992248062015 27.85018773777414 23.093774397797922 40-41 23.30077099972328 25.91343905953301 27.757540625387644 23.02824931535606 42-43 23.13189161507588 25.97882500669966 27.44062833417134 23.448655044053123 44-45 23.247123714236007 26.053329508547655 27.499699895924145 23.199846881292192 46-47 23.48874608814864 26.346235362181584 27.07473712549811 23.09028142417166 48-49 23.47715287717064 26.26583668391368 27.091172704713216 23.165837734202462 50-51 23.207599497452897 26.51554134557369 27.150097759923014 23.126761397050398 52-53 23.513651819295806 26.48229060901287 26.77037819909693 23.233679372594395 54-55 23.208182736973736 26.281124076285984 27.030190070603787 23.480503116136493 56-57 23.263727923578713 26.50745247349684 26.94366631013389 23.285153292790554 58-59 23.503904786145004 26.172193209090388 27.001097047037533 23.322804957727072 60-61 23.322553285649327 26.205721270076083 27.343265474590346 23.128459969684243 62-63 23.06674684305472 26.35452757972301 27.378542316906717 23.20018326031555 64-65 22.97921257557067 26.333705378454862 27.212912607269296 23.474169438705168 66-67 23.002470727253197 26.386268963130068 27.000919062794665 23.610341246822074 68-69 22.998424950202214 26.24543415816728 27.28262247581975 23.47351841581076 70-71 23.12806097175222 26.2318908047006 27.047752054573778 23.5922961689734 72-73 23.120920766369572 26.2157077021278 27.150276209859335 23.51309532164329 74-75 22.95795057390042 26.718788793185766 26.768396325509443 23.554864307404365 76-77 23.151982769978062 26.37447325661544 26.82713365677812 23.646410316628383 78-79 23.198516779292177 26.412233230252358 26.895592333829647 23.493657656625818 80-81 23.190275660708544 26.575091557610424 26.8505518272866 23.38408095439443 82-83 23.248294700888316 26.44486839925979 26.836451183172823 23.47038571667907 84-85 23.227579570681314 26.521237542880083 26.725525002064355 23.52565788437425 86-87 23.243944256805133 26.475177514849385 26.837199933557226 23.44367829478825 88-89 23.137108597196505 26.429950051104168 26.680792518024905 23.752148833674426 90-91 23.120067494367113 26.43811082617097 26.758608802438122 23.68321287702379 92-93 23.247419038197854 26.240709301392933 26.88532909888817 23.62654256152104 94-95 23.165345894593507 26.185639217715885 26.924891214152236 23.724123673538376 96-97 23.168864428814995 26.053137797036875 27.022376447794414 23.75562132635371 98-99 23.02305585433845 26.249648502417394 26.724176206633317 24.00311943661084 100-101 23.357377474883013 25.567939617079027 27.13360131786909 23.94108159016887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2037.0 1 1144.5 2 290.0 3 339.5 4 333.0 5 319.0 6 467.5 7 955.0 8 1582.5 9 1890.0 10 1837.5 11 1808.5 12 1940.0 13 2104.5 14 2288.0 15 2491.0 16 2626.5 17 2753.0 18 2924.5 19 3067.0 20 3239.0 21 3642.5 22 4281.5 23 5076.5 24 6092.0 25 7506.5 26 9497.5 27 11865.0 28 14181.5 29 16632.5 30 19304.5 31 22549.5 32 26530.5 33 29477.5 34 31933.5 35 34474.0 36 37796.0 37 42036.0 38 45734.0 39 48361.5 40 51582.5 41 56545.0 42 60719.0 43 64564.5 44 68701.0 45 72943.0 46 75945.5 47 80759.0 48 88690.0 49 89545.0 50 84597.0 51 81999.0 52 80973.0 53 81568.5 54 81409.5 55 76611.0 56 70016.0 57 63813.0 58 58447.5 59 52190.5 60 43957.0 61 36481.5 62 30254.5 63 23873.5 64 18241.0 65 13901.0 66 10452.5 67 8432.0 68 6512.0 69 4698.5 70 3607.0 71 2732.0 72 2076.0 73 1671.5 74 1299.5 75 995.5 76 726.0 77 440.5 78 283.5 79 209.5 80 139.5 81 99.5 82 66.0 83 44.5 84 38.0 85 30.0 86 19.5 87 20.5 88 18.0 89 11.0 90 8.5 91 9.5 92 10.0 93 8.0 94 6.5 95 5.0 96 3.5 97 6.0 98 5.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34487410029229515 2 0.12458404697241292 3 0.11612962060524193 4 0.12520150507788047 5 0.14158789326144222 6 0.09076634150372898 7 0.09841332265605779 8 0.10473039404276421 9 0.0925237222654443 10-11 0.09124130927716555 12-13 0.09508854824200179 14-15 0.09627596767559321 16-17 0.0861354057127224 18-19 0.10043193569316321 20-21 0.1102875169919721 22-23 0.118338220751722 24-25 0.1319460474606798 26-27 0.13101986030247847 28-29 0.12600895029272263 30-31 0.11712705292945874 32-33 0.10964631049783273 34-35 0.336585912645827 36-37 0.4994761105458994 38-39 0.47164299902251633 40-41 0.44770462324131305 42-43 0.4821635352041364 44-45 0.4891930582509977 46-47 0.4982411943349644 48-49 0.5103053757802534 50-51 0.4976712330068405 52-53 0.5014234784169894 54-55 0.48748317426662596 56-57 0.4635447984854228 58-59 0.44288370034093183 60-61 0.4026064331535105 62-63 0.4762026896475074 64-65 0.507170588475572 66-67 0.5168124342763344 68-69 0.5313226997548217 70-71 0.5221558217274958 72-73 0.513107685643529 74-75 0.5208496603505453 76-77 0.4967212974599673 78-79 0.473352883006888 80-81 0.475157760545947 82-83 0.490119245409199 84-85 0.490119245409199 86-87 0.49254158105372553 88-89 0.5065531303701044 90-91 0.5228920217763224 92-93 0.5147700728505571 94-95 0.5188547957021116 96-97 0.5189972860341426 98-99 0.5128227049794671 100-101 0.4991436331044939 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2105406.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.83949864821001 #Duplication Level Percentage of deduplicated Percentage of total 1 79.36081294692156 48.28272072005082 2 12.043466727165258 14.654369553342661 3 3.6975847477901747 6.748776067944669 4 1.630003399176231 3.9667435840304015 5 0.8753990480911683 2.6629419601493485 6 0.5351099190091748 1.953349151652148 7 0.3519109966719517 1.498706202441941 8 0.2489925538659182 1.2118865715471911 9 0.18745193697418716 1.0264033679530868 >10 0.9821234399621175 10.640600279396946 >50 0.057265375760405 2.364430876549116 >100 0.027143047644723226 3.072251779874615 >500 0.002110521317480916 0.8955400003895417 >1k 5.471721934209529E-4 0.7644262103054011 >5k 7.816745620299328E-5 0.2568536743722453 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5401 0.2565300944330927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4409 0.20941329130818473 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3252 0.15445951992157333 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2757 0.130948615136463 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.749677734365723E-5 0.0 2 0.0 0.0 0.0 4.749677734365723E-5 0.0 3 0.0 0.0 0.0 4.749677734365723E-5 0.0 4 0.0 0.0 0.0 4.749677734365723E-5 0.0 5 0.0 0.0 0.0 4.749677734365723E-5 4.749677734365723E-5 6 0.0 0.0 0.0 4.749677734365723E-5 4.749677734365723E-5 7 0.0 0.0 0.0 4.749677734365723E-5 4.749677734365723E-5 8 0.0 0.0 0.0 4.749677734365723E-5 4.749677734365723E-5 9 0.0 0.0 0.0 4.749677734365723E-5 4.749677734365723E-5 10-11 0.0 0.0 0.0 7.124516601548585E-5 4.749677734365723E-5 12-13 0.0 0.0 0.0 9.499355468731446E-5 1.424903320309717E-4 14-15 0.0 0.0 0.0 9.499355468731446E-5 2.1373549804645754E-4 16-17 0.0 0.0 0.0 9.499355468731446E-5 2.3748388671828618E-4 18-19 0.0 0.0 0.0 1.424903320309717E-4 2.3748388671828618E-4 20-21 0.0 0.0 0.0 1.8998710937462892E-4 2.3748388671828618E-4 22-23 0.0 0.0 0.0 3.324774414056006E-4 2.3748388671828618E-4 24-25 0.0 0.0 0.0 3.7997421874925785E-4 2.3748388671828618E-4 26-27 0.0 0.0 0.0 5.699613281238868E-4 2.849806640619434E-4 28-29 0.0 0.0 0.0 0.00223234853515189 2.849806640619434E-4 30-31 0.0 0.0 0.0 0.0073857488769387 2.849806640619434E-4 32-33 0.0 0.0 0.0 0.016148904296843458 3.324774414056006E-4 34-35 0.0 0.0 0.0 0.02902053095697457 3.7997421874925785E-4 36-37 0.0 0.0 0.0 0.05162899697255541 3.7997421874925785E-4 38-39 0.0 0.0 0.0 0.08566043793928582 3.7997421874925785E-4 40-41 0.0 0.0 0.0 0.12121177578101325 3.7997421874925785E-4 42-43 0.0 0.0 0.0 0.15930419121062636 3.7997421874925785E-4 44-45 0.0 0.0 0.0 0.2071334459956892 4.0372260742108643E-4 46-47 0.0 0.0 0.0 0.2688555081537718 4.2747099609291507E-4 48-49 0.0 0.0 0.0 0.3328336672356781 4.2747099609291507E-4 50-51 0.0 0.0 0.0 0.4010627878898417 4.7496777343657235E-4 52-53 0.0 0.0 0.0 0.4709305473623615 4.7496777343657235E-4 54-55 0.0 0.0 0.0 0.552625004393452 4.7496777343657235E-4 56-57 0.0 0.0 0.0 0.6542681079088783 4.7496777343657235E-4 58-59 0.0 0.0 0.0 0.7667404766586587 4.987161621084009E-4 60-61 0.0 0.0 0.0 0.8909445494123224 5.224645507802295E-4 62-63 0.0 0.0 0.0 1.0188296224101194 5.224645507802295E-4 64-65 0.0 0.0 0.0 1.1560240637672734 5.699613281238868E-4 66-67 0.0 0.0 0.0 1.316824403464225 6.174581054675441E-4 68-69 0.0 0.0 0.0 1.5033917448701106 6.174581054675441E-4 70-71 0.0 0.0 0.0 1.7070104293423691 6.174581054675441E-4 72-73 0.0 0.0 0.0 1.9214583790489814 6.174581054675441E-4 74-75 0.0 2.3748388671828615E-5 0.0 2.1416771872028484 6.174581054675441E-4 76-77 0.0 4.749677734365723E-5 0.0 2.387520506733618 6.174581054675441E-4 78-79 0.0 4.749677734365723E-5 0.0 2.675493467768212 6.174581054675441E-4 80-81 0.0 4.749677734365723E-5 0.0 2.989423417621114 6.174581054675441E-4 82-83 0.0 4.749677734365723E-5 0.0 3.310287896966191 6.174581054675441E-4 84-85 0.0 4.749677734365723E-5 0.0 3.6399867768971874 6.174581054675441E-4 86-87 0.0 4.749677734365723E-5 0.0 3.9923653680097804 6.412064941393727E-4 88-89 0.0 4.749677734365723E-5 0.0 4.383192600382064 6.649548828112012E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7380 0.0 25.989904 1 GTATCAA 10070 0.0 25.789179 1 GTACATG 7585 0.0 24.411173 1 TACATGG 7480 0.0 24.002449 2 TATACGG 120 1.7431729E-4 23.685797 5 ACATGGG 7895 0.0 22.260748 3 ATCAACG 11800 0.0 21.79896 3 CAACGCA 11905 0.0 21.765862 5 TCAACGC 11925 0.0 21.610184 4 GAGTACT 5695 0.0 21.535175 12-13 TATCAAC 11965 0.0 21.498348 2 AACGCAG 12130 0.0 21.39253 6 GTACCGT 210 8.8758316E-7 20.293903 6 GTACTTT 6075 0.0 19.87728 14-15 TGTACCG 290 2.5247573E-9 19.602037 5 AGAGTAC 9700 0.0 19.233194 10-11 ACGCAGA 13425 0.0 19.18788 7 CGCAGAG 13550 0.0 18.905582 8 TACCTGG 1870 0.0 18.492653 2 AGTACTT 5945 0.0 18.479004 12-13 >>END_MODULE